Liquid storage module for biochemical reaction and biochemical reaction system

Abstract

The invention relates to a biochemical reaction liquid storage module and a biochemical reaction system, the biochemical reaction liquid storage module comprises a housing, a lifting assembly, a cleaning assembly, a purging assembly and a liquid discharge assembly, the housing defines a storage bin, the lifting assembly is arranged on the outer side of the housing, and the lifting assembly is used for driving a reagent needle to extend into or leave the storage bin; the cleaning assembly is used for cleaning the outer wall of the reagent needle; the purging assembly is used for purging residual liquid on the reagent needle and in the storage bin; the liquid discharging assembly is used for collecting and discharging waste liquid. According to the liquid storage module for the biochemical reaction, through the cleaning assembly, the purging assembly and the liquid discharging assembly, automatic cleaning of the outer wall of the reagent needle and automatic discharging of residual waste liquid in the reagent needle and the storage bin can be achieved, the self-cleaning function can be achieved, the cleanliness of the storage bin can be improved, cross contamination of reagents can be reduced, and then the quality of the biochemical reaction is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biochemical substance analysis, and in particular to a biochemical reaction liquid storage module and a biochemical reaction system. BACKGROUND

[0002] With the continuous maturity of sequencing technology, genetic sequencing has broad application prospects in the fields of disease early screening, microorganisms, and genetic testing, and the entire industry has begun to develop rapidly, and the market demand for sequencers is also increasing.

[0003] As the core module of a genetic sequencer, the reagent storage module is mainly used to store reagents and cleaning solutions for sequencing. During the use of the sequencer, the reagent needle needs to enter the reagent solution to extract the reagent. After extraction is completed, the reagent needle surface will be left with reagent, which is easy to cause cross contamination and affect sequencing quality. In addition, during the reagent storage and sequencing work, the reagent bin will continuously perform refrigeration work. When the reagent bin door is frequently opened and closed, the cold air in the reagent bin and the hot air outside the reagent bin will contact, causing condensate water to be generated at the reagent bin, reagent needle, and other parts. On the one hand, this will affect the refrigeration effect, and on the other hand, if condensate water is generated on the surface of the reagent needle or the reagent box, it will cause reagent contamination, thereby affecting sequencing quality. SUMMARY

[0004] In order to solve at least one of the above defects, it is necessary to provide a biochemical reaction liquid storage module, which can realize automatic cleaning of the reagent needle and the storage bin, improve the cleanliness of the storage bin and the reagent needle, reduce cross contamination, and improve the quality of biochemical reactions.

[0005] In addition, the present application also provides a biochemical reaction system using the aforementioned biochemical reaction liquid storage module.

[0006] In a first aspect, the embodiments of the present application provide a biochemical reaction liquid storage module, which comprises: a shell, a lifting assembly, a cleaning assembly, a purging assembly, and a liquid discharge assembly. The shell encloses a storage bin for accommodating liquid required for biochemical reactions. The lifting assembly is arranged outside the shell and is used to drive a reagent needle to extend into or leave the storage bin to extract the liquid. The cleaning assembly is used to provide cleaning liquid to the reagent needle located on the lifting assembly to clean the outer wall of the reagent needle. The purging assembly is used to blow gas to the reagent needle and the storage bin to purge waste liquid on the reagent needle and in the storage bin to the bottom of the storage bin. The liquid discharge assembly is used to collect the waste liquid at the bottom of the storage bin and discharge the liquid out of the storage bin.

[0007] In some possible embodiments, the lifting assembly comprises a lifting driving mechanism arranged outside the shell, a mounting plate arranged on the lifting driving mechanism, and a plurality of mounting blocks arranged on the mounting plate, the mounting blocks comprising first mounting holes for mounting the reagent needles, and the lifting driving mechanism being configured to drive the mounting plate to move towards or away from the storage bin, so that the reagent needles mounted in the first mounting holes extend into or out of the storage bin.

[0008] In some possible embodiments, the cleaning assembly comprises a cleaning pipeline, and the mounting blocks further comprise second mounting holes arranged around the first mounting holes and in communication with the first mounting holes, one end of the cleaning pipeline being mounted in the second mounting holes, and the cleaning pipeline being configured to supply cleaning liquid to the reagent needles located in the first mounting holes.

[0009] In some possible embodiments, the biochemical reaction reagent storage module further comprises a reagent needle fixing assembly, the reagent needle fixing assembly comprising a fixing plate arranged on a side of the storage bin close to the lifting assembly, and a plurality of hollow fixing sleeves arranged between the fixing plate and the shell, and the reagent needles located on the lifting assembly being capable of extending into the storage bin through the shell, the fixing sleeves and the fixing plate.

[0010] In some possible embodiments, the blowing assembly comprises a blowing pipeline, and the blowing pipeline comprises a plurality of blowing branches, one end of each of the blowing branches being in communication with a corresponding fixing sleeve.

[0011] In some possible embodiments, the liquid discharging assembly comprises a flow collecting plate arranged at the bottom of the storage bin, a liquid discharging hole arranged at one end of the flow collecting plate, and a liquid discharging pipeline in communication with the liquid discharging hole, the flow collecting plate comprising a bottom plate close to the bottom wall of the shell, the bottom plate comprising oppositely arranged first and second ends, and the distance between the bottom plate and the bottom wall of the shell decreasing from the first end to the second end, so that the bottom plate is arranged in an inclined manner, and the liquid discharging hole is arranged at the second end.

[0012] In some possible embodiments, the liquid discharging assembly further comprises a baffle plate arranged at the second end, the baffle plate and the bottom plate surrounding a flow collecting groove, and an obtuse angle being formed between the baffle plate and the bottom plate.

[0013] In some possible embodiments, the flow collecting plate further comprises a top plate arranged opposite to the bottom plate, and a side plate connecting the top plate and the bottom plate, the top plate, the bottom plate and the side plate surrounding a flow collecting cavity, and an opening being arranged on the top plate, and the storage bin being in communication with the flow collecting cavity through the opening.

[0014] In some possible embodiments, the biochemical reaction liquid storage module further comprises a temperature control assembly for adjusting the temperature in the storage chamber.

[0015] In a second aspect, the embodiments of the present application provide a biochemical reaction system, comprising: a biochemical reaction platform and a biochemical reaction liquid storage module as described above, the biochemical reaction liquid storage module being configured to provide liquid required by the biochemical reaction to the biochemical reaction platform through the reagent needle.

[0016] The biochemical reaction liquid storage module provided by the embodiments of the present application can realize automatic cleaning of the outer wall of the reagent needle after extracting the liquid, can realize blowing of waste liquid such as residual cleaning liquid on the outer wall of the reagent needle and residual liquid in the storage chamber (for example, condensate and dripping cleaning liquid, etc.) to facilitate the discharge of the waste liquid, and can realize automatic discharge of the waste liquid in the storage chamber through the liquid discharge assembly. Therefore, the biochemical reaction liquid storage module can realize self-cleaning function, can effectively remove residual waste liquid (for example, biochemical reaction liquid, cleaning liquid, condensate generated by condensation, etc.) on the surface of the reagent needle and in the storage chamber, improve the cleanliness of the storage chamber, reduce cross contamination of the biochemical reaction liquid, and further improve the quality of the biochemical reaction. In addition, the biochemical reaction liquid storage module has simple structure, occupies small space, has high space utilization rate, is conducive to miniaturization of the biochemical reaction liquid storage module, and has low cost. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0018] Figure 1 is a system framework diagram of a biochemical reaction system in an embodiment of the present application.

[0019] Figure 2 is a framework diagram of a biochemical reaction liquid storage module in an embodiment of the present application.

[0020] Figure 3 is a structure schematic diagram of a biochemical reaction liquid storage module in an embodiment of the present application.

[0021] Figure 4 is a structure schematic diagram of a cleaning assembly cleaning a reagent needle in an embodiment of the present application.

[0022] Figure 5 is a structure schematic diagram of a blowing assembly blowing a reagent needle and a storage chamber in an embodiment of the present application.

[0023] Figure 6 is a structural schematic diagram of a liquid discharge assembly in an embodiment of the present application.

[0024] Figure 7 is a working process schematic diagram of liquid extraction in an embodiment of the present application.

[0025] Figure 8 is a working process schematic diagram of cleaning a reagent needle in an embodiment of the present application.

[0026] Figure 9 is a schematic diagram of a purging process in an embodiment of the present application.

[0027] Main component symbol description

[0028]

[0029]

[0030]

[0031] The following detailed description will further describe the present application with reference to the above-mentioned drawings. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0033] It should be noted that when a component is referred to as being "fixed" or "mounted" to another component, it can be directly on the other component or there can be an intermediate component between them. When a component is referred to as being "disposed" on another component, it can be directly disposed on the other component or there can be an intermediate component between them. The term "and / or" as used herein includes all possible combinations of one or more of the associated listed items.

[0034] Please refer to Figure 1This application also provides a biochemical reaction system 1000, which may include a biochemical reaction platform 200 and a biochemical reaction liquid storage module 100. The biochemical reaction liquid storage module 100 is used to provide the biochemical reaction platform 200 with the liquid required for the biochemical reaction to realize the biochemical reaction process. The liquid may be, for example, a biological sample (e.g., human blood sample, tissue sample, or saliva sample), reagents, buffer solutions, cleaning solutions, etc., required for the biochemical reaction, but is not limited to these. The biochemical reaction system 1000 may be, for example, a gene sequencing system, but is not limited to these. The biochemical reaction liquid storage module 100 can be used to store biological samples, various reagents, buffer solutions, and cleaning solutions required for gene sequencing, and provide these liquids to the gene sequencing reaction platform to realize the sequencing process.

[0035] Please refer to both together. Figure 2 and Figure 3 This application provides a biochemical reaction liquid storage module 100, which can be used for the aforementioned liquid. The biochemical reaction liquid storage module 100 includes: a shell 1, a lifting assembly 2, a cleaning assembly 3, a purging assembly 4, and a draining assembly 5. The shell 1 forms a storage chamber 6 for storing the aforementioned liquid. The lifting assembly 2 is located on the outside of the shell 1 and is used to drive a reagent needle 10 into or out of the storage chamber 6 to extract the liquid and provide it to the biochemical reaction platform 200 through a pipeline to complete the biochemical reaction. The cleaning assembly 3 is used to provide cleaning fluid to the reagent needle 10 located on the lifting assembly 2 to clean the outer wall of the reagent needle 10. The purging assembly 4 is used to blow air into the reagent needle 10 and the storage chamber 6 to purge the waste liquid on the reagent needle 10 and in the storage chamber 6 to the bottom of the storage chamber 6. Specifically, the waste liquid may include residual biochemical reaction liquid, cleaning liquid, and condensate on the outer wall of the reagent needle 10, as well as residual biochemical reaction liquid, cleaning liquid, and condensate in the storage chamber 6. The draining assembly 5 is used to collect the waste liquid at the bottom of the storage chamber 6 and drain it from the storage chamber 6. This biochemical reaction liquid storage module 100 can provide the aforementioned liquid to the biochemical reaction platform 200 in the biochemical reaction system 1000 to complete the biochemical reaction process. During liquid transfer, the biochemical reaction liquid storage module 100 can achieve self-cleaning of the waste liquid on the surface of the reagent needle 10 and the waste liquid in the storage chamber 6, thereby reducing the risk of cross-contamination of the biochemical reaction liquid and improving the quality of the biochemical reaction.

[0036] Please see Figure 2 and Figure 3The housing 1 includes a top wall 11, a bottom wall 12 opposite to the top wall 11, and a side wall 13 connecting the top wall 11 and the bottom wall 12. The top wall 11, the bottom wall 12, and the side wall 13 together form the storage compartment 6. The housing 1 also includes an openable and closable door 14, which is located on the side wall 13 and is used to open the storage compartment 6.

[0037] In some embodiments, the liquid can be contained in a storage box 20, which is detachably disposed within a storage chamber 6. Specifically, the storage box 20 can be placed into or removed from the storage chamber 6 by opening the chamber door 14. After the storage box 20 is placed into the storage chamber 6, the reagent needle 10 can be driven into the storage box 20 by the lifting assembly 2 to extract the liquid for the biochemical reaction. After the extraction of the liquid for the biochemical reaction is completed, the chamber door 14 can be opened to remove the storage box 20.

[0038] In some embodiments, the storage compartment 6 is also used to contain a waste liquid container 30, which is used to collect waste liquid from cleaning the reagent needle 10. When the reagent needle 10 needs to be cleaned, the waste liquid container 30 is placed in the storage compartment 6, the lifting assembly 2 drives the reagent needle 10 to extend into the waste liquid container 30, and the cleaning assembly 3 provides cleaning fluid to the reagent needle 10 to clean its surface. The cleaning waste liquid flows down the reagent needle 10 into the waste liquid container 30 for collection. After cleaning, the waste liquid container 30 can be removed for further processing. Of course, the waste liquid container 30 can be a used reagent storage box that is to be discarded.

[0039] Please see Figures 2 to 4 The lifting assembly 2 includes: a lifting drive mechanism 21 disposed on the outside of the housing 1, a mounting plate 22 disposed on the lifting drive mechanism 21, and a plurality of mounting blocks 23 disposed on the mounting plate 22. Each mounting block 23 has a first mounting hole 24 for mounting the reagent needle 10. Specifically, the lifting drive mechanism 21 is mounted on the top wall 11. The lifting drive mechanism 21 drives the mounting plate 22 to move toward or away from the storage chamber 6, so that the reagent needle 10 located in the first mounting hole 24 can extend into or out of the storage chamber 6. Specifically, the lifting drive mechanism 21 can drive the reagent needle 10 to reciprocate up and down, thereby enabling it to extend into or out of the storage chamber 6.

[0040] In some embodiments, multiple mounting blocks 23 can be arranged in an array to mount multiple reagent needles 10 for transferring various liquids. Each mounting block 23 can mount one or more reagent needles 10. In this embodiment, a first mounting hole 24 is formed on the mounting block 23 to mount one reagent needle 10.

[0041] In some embodiments, the reagent needle 10 is detachably disposed within the first mounting hole 24 to facilitate the replacement and maintenance of the reagent needle 10.

[0042] Please refer to it again. Figures 2 to 4 The cleaning assembly 3 includes a cleaning conduit 31. The mounting block 23 also has a second mounting hole 25, which is located around and communicates with the first mounting hole 24. One end of the cleaning conduit 31 extends into the second mounting hole 25 and is used to provide cleaning fluid to the reagent needle 10 located within the first mounting hole 24. The first mounting hole 24 and the second mounting hole 25 on the mounting block 23 allow the reagent needle 10 and the cleaning conduit 31 to form an integrated structure, facilitating direct cleaning of the outer surface of the reagent needle 10. It is understood that a driving mechanism such as an injection pump can inject the cleaning fluid from the cleaning fluid container 32 into the cleaning conduit 31 to clean the surface of the reagent needle 10.

[0043] In some embodiments, the first mounting hole 24 extends vertically, while the second mounting hole 25 is inclined, such that the central axis of the second mounting hole 25 forms a certain angle with the central axis of the first mounting hole 24. The second mounting hole 25 is inclined relative to the vertical direction on the mounting block 23, which facilitates the access of the cleaning pipe 31 and the communication with the first mounting hole 24.

[0044] In some embodiments, the cleaning pipe 31 is detachably disposed within the second mounting hole 25 to facilitate the replacement and maintenance of the cleaning pipe 31.

[0045] In some embodiments, the cleaning assembly 3 may further include a cleaning fluid container 32 connected to the cleaning pipeline 31, the cleaning fluid container 32 being used to supply fluid to the cleaning pipeline 31. It is understood that the cleaning fluid container 32 may be part of the biochemical reaction liquid storage module 100, or it may not be part of the biochemical reaction liquid storage module 100, but rather used as a consumable. It is also understood that the cleaning fluid container 32 may not be provided, and the cleaning pipeline 31 may be directly connected to the pure water interface during the cleaning process.

[0046] In some embodiments, the cleaning pipeline 31 may include a cleaning needle 33 installed in the second mounting hole 25 and a hose 34 connecting the cleaning needle 33 and the cleaning liquid box 32.

[0047] Please see Figure 3 and Figure 5The biochemical reaction liquid storage module 100 further includes a reagent needle fixing assembly 7 located within the storage chamber 6 and near the lifting assembly 2. The reagent needle fixing assembly 7 is used to fix the reagent needle 10. In some embodiments, the reagent needle fixing assembly 7 includes a fixing plate 71 located within the storage chamber 6 and near the lifting assembly 2, and a plurality of hollow fixing sleeves 72 located between the fixing plate 71 and the housing 1. The reagent needle 10 located on the lifting assembly 2 can pass through the housing 1, the fixing sleeves 72, and the fixing plate 71 to extend into the storage chamber 6. The housing 1 and the fixing plate 71 each have through holes (not shown) corresponding to the fixing sleeves 72 to allow the reagent needle 10 to pass through. Specifically, the fixing plate 71 is located near the top wall 11, and the fixing sleeves 72 are located between the fixing plate 71 and the top wall 11, communicating with the through holes in the top wall 11 and the fixing plate 71. The inner diameter of the through holes on the fixing sleeve 72, housing 1, and fixing plate 71 can be appropriately large in order to flush the outer wall of the reagent needle 10.

[0048] Please see Figure 2 , Figure 3 and Figure 5 The purging assembly 4 includes an air blowing pipe 41. Specifically, the air blowing pipe 41 may include multiple air blowing branches 42. One end of each air blowing branch 42 is connected to a corresponding fixed sleeve 72, so that air can be blown into the fixed sleeve 72. The gas can blow away the residual liquid on the reagent needle 10. At the same time, the gas can further enter the storage chamber 6 through the through holes of the fixed sleeve 72 and the fixed plate 71 to purge the residual liquid in the storage chamber 6.

[0049] In some embodiments, the purging assembly 4 may further include an air source 43 connected to the air blowing line 41, which can supply clean air to the air blowing line 41 and control the purging force by controlling the gas pressure. It is understood that the air source 43 may be part of the biochemical reaction liquid storage module 100 or may be independent of the biochemical reaction liquid storage module 100.

[0050] In some embodiments, the air source 43 may be an air pump.

[0051] Please see Figures 2 to 4 and Figure 6The drainage assembly 5 includes: a collection plate 51 disposed at the bottom of the storage compartment 6, a drainage hole 52 disposed at one end of the collection plate 51, a drainage pipe 53 communicating with the drainage hole 52, and a drainage drive mechanism 54 communicating with the drainage pipe 53. The collection plate 51 includes a top plate 511 near the storage compartment 6, a bottom plate 512 disposed opposite to the top plate 511, and a side plate 513 connecting the top plate 511 and the bottom plate 512. The top plate 511, the bottom plate 512, and the side plate 513 form a collection cavity 55. The top plate 511 has an opening (not shown in the figure), and the storage compartment 6 communicates with the collection cavity 55 through the opening. The base plate 512 includes a first end 514 and a second end 515 disposed opposite to each other. The distance between the base plate 512 and the top plate 511 increases sequentially from the first end 514 to the second end 515, causing the base plate 512 to be inclined. The drain hole 52 is located at the second end 515. Specifically, the waste liquid blown to the bottom of the storage chamber 6 by the purging assembly 4 enters the collecting cavity 55 of the collecting plate 51 through the opening, and is guided along the inclined base plate 512 to the second end 515 of the base plate 512, and is automatically discharged from the storage chamber 6 through the drain hole 52 and the drain pipe 53.

[0052] In some embodiments, a drainage drive mechanism 54, such as a water pump, can be connected to the drainage pipe 53 to extract waste liquid through the drainage hole 52 and the drainage pipe 53, thereby improving drainage efficiency. It is understood that the drainage drive mechanism 54 may be part of the biochemical reaction liquid storage module 100 or may not be part of the biochemical reaction liquid storage module 100. When needed, the drainage pipe 53 can be connected to an existing drainage drive mechanism 54 (such as a water pump).

[0053] In some embodiments, the drainage assembly 5 further includes a baffle 56 disposed at the second end 515, the baffle 56 and the bottom plate 512 forming a collection trough 57 for collecting waste liquid. Specifically, the baffle 56 and the bottom plate 512 may form an obtuse angle to increase the volume of the collection trough 57, thereby accommodating more waste liquid.

[0054] Please refer to it again. Figure 3 The biochemical reaction liquid storage module 100 further includes a temperature control component 8, which can be used to regulate the temperature inside the storage chamber 6. For example, the temperature control component 8 can be a cooling component to cool the liquid inside the storage chamber 6. It is understood that the temperature control component 8 can also be a heating component to keep the liquid inside the storage chamber 6 warm.

[0055] like Figures 1 to 6 As shown, the use of the biochemical reaction liquid storage module 100 in the sequencing system specifically includes the following steps:

[0056] Step 1: After the biochemical reaction liquid storage module 100 starts working, the temperature control component 8 (specifically, the refrigeration component) cools the storage chamber 6. The chamber door 14 is opened, the storage box 20 is placed into the storage chamber 6, and the door 14 is closed. During the opening and closing of the door 14, condensate will be generated inside the storage chamber 6. The condensate is collected by the manifold 51 in the drainage component 5, passes through the drainage hole 52 and the drainage pipe 53, and is discharged from the storage chamber 6 under the action of the drainage drive mechanism 54 (specifically, a water pump).

[0057] Step 2: The reagent needle 10, driven by the lifting drive mechanism 21, extends into the storage box 20. Under the action of external force (e.g., suction force), the biological sample and various reagents in the storage box 20 are extracted through the reagent needle 10 and enter the biochemical reaction platform 200 (e.g., ...). Figure 1 As shown in the figure, sequencing is thus achieved.

[0058] Step 3: After sequencing is completed, replace storage box 20 with waste liquid box 30 (or continue to use the original storage box 20), and use external force to deliver the cleaning solution in cleaning solution box 32 to the surface of reagent needle 10 through cleaning tubing 31 to rinse the residual biochemical reaction solution on reagent needle 10.

[0059] Step 4: After the reagent needle 10 is rinsed, the reagent needle 10 is moved out of the waste liquid box 30 under the drive of the lifting drive mechanism 21, and the waste liquid box 30 is taken out for disposal.

[0060] Step 5: Excess cleaning fluid and condensate remaining on the surface of reagent needle 10 are collected by manifold 51, and discharged from storage chamber 6 through drain hole 52 and drain pipe 53 under the action of drain drive mechanism 54 (e.g., water pump).

[0061] Step 6: After cleaning, the reagent needle 10 moves up and down at a constant speed under the drive of the lifting mechanism 21.

[0062] Step 7: The air source 43 starts working, and delivers compressed air through the air blowing pipe 41 to the storage chamber 6 to purge the liquid remaining on the surface of the reagent needle 10 and the condensate and other waste liquids inside the storage chamber 6.

[0063] Step 8: The residual liquid on the surface of the reagent needle 10 and the condensate and other waste liquid inside the storage chamber 6 drip down to the bottom of the storage chamber 6 under the action of compressed air. The liquid is collected by the manifold 51 and discharged from the storage chamber 6 through the drain pipe 53 under the action of the drain drive mechanism 54.

[0064] The following describes the different control logics in detail, thereby further explaining the use of the aforementioned biochemical reaction liquid storage module 100.

[0065] Please see Figure 7 Please refer to both together. Figure 3 and Figure 6 The reagent extraction process specifically includes the following steps:

[0066] Reagent extraction process: When the biochemical reaction liquid storage module 100 is powered on, the temperature control component 8 (cooling component) and the drainage pipeline 53 start working simultaneously and continue throughout the entire process. Meanwhile, under external force, the reagent extraction pipeline extracts the reagent from the storage box 20 within the storage chamber 6 via the reagent needle 10, thus realizing the pumping function during the sequencing process.

[0067] During continuous operation of the refrigeration unit, the condensate generated in the storage compartment 6 is eventually collected at the manifold 51 and discharged from the storage compartment 6 by a water pump through the drain pipe 53.

[0068] Please see Figure 8 Please refer to both together. Figure 3 and Figure 4 The pipeline cleaning process specifically includes the following steps:

[0069] Reagent needle cleaning process: After completing the reagent extraction process, the waste liquid box 30 is replaced and the cleaning liquid box 32 is switched. The water washing solution in the cleaning liquid box 32 is injected into the surface of the reagent needle 10 through the cleaning pipeline 31 by the injection pump to rinse the residual biochemical reaction liquid on the surface of the reagent needle 10. Most of the waste liquid after rinsing flows into the waste liquid box 30. A small amount of cleaning liquid dripping from the surface of the reagent needle 10 can be collected by the manifold 51 and discharged into the storage chamber 6.

[0070] Meanwhile, the refrigeration components continue to work, and the condensate generated in the storage compartment 6 is eventually collected at the manifold 51 and discharged from the storage compartment 6 by the water pump through the drain pipe 53.

[0071] Please see Figure 9 Please refer to both together. Figure 3 and Figure 5 The specific steps of the air blowing pipeline process include:

[0072] Purging process of storage chamber 6: After the cleaning process of reagent needle 10 is completed, there is residual cleaning fluid and condensate on the surface of reagent needle 10 and inside storage chamber 6. Compressed air is introduced into the storage chamber 6 through the air blowing pipe 41 to purge the surface of reagent needle 10 and the inside of storage chamber 6, blowing away the residual liquid, which is then discharged and recycled through the drain assembly 5.

[0073] Meanwhile, the refrigeration components continue to work, and the condensate generated in the storage compartment 6 is eventually collected at the manifold 51 and discharged from the storage compartment 6 by the water pump through the drain pipe 53.

[0074] After the air blowing line 41 starts working, the air pump blows gas into the storage chamber 6 through the air blowing branch 42 to purge the condensate inside the storage chamber 6; at the same time, the reagent needle 10 moves upward under the drive of the lifting drive mechanism 21. During the movement, the purging gas purges the residual liquid on the surface of the reagent needle 10.

[0075] The biochemical reaction liquid storage module 100 of this application embodiment is a self-cleaning biochemical reaction liquid storage module. Its main functions consist of three major cleaning components: a cleaning component 3, a purging component 4, and a draining component 5. The cleaning component 3 is mainly responsible for rinsing the outer wall of the reagent needle 10 with residual biochemical reaction liquid (such as reagents required for biochemical reactions) to ensure that there is no liquid residue on the outer surface of the reagent needle 10 and reduce cross-contamination. The purging component 4 is mainly responsible for purging the inner cavity of the storage chamber 6 and the surface of the reagent needle 10 to remove residual condensate, cleaning fluid, and other waste liquids from the surface of the reagent needle 10 and the inner surface of the storage chamber 6, thereby reducing contamination of the biochemical reaction liquid. The draining component 5 is mainly responsible for collecting and draining the condensate, dripping cleaning fluid, and other waste liquids collected at the bottom of the storage chamber 6, thereby reducing the accumulation of waste liquid, especially condensate, inside the storage chamber 6 and reducing contamination of the biochemical reaction liquid. Through the coordinated operation of the three main cleaning components and the orderly interaction of control logic, the biochemical reaction liquid storage module 100 can autonomously and efficiently perform automatic cleaning, improving the cleanliness of the liquid usage environment, reducing cross-contamination, and thus improving the quality of the biochemical reaction. Furthermore, the biochemical reaction liquid storage module 100 has a simple structure, occupies little space, and has high space utilization, which is conducive to its miniaturization and low cost.

[0076] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A liquid storage module for biochemical reactions, characterized in that, include: The shell surrounds a storage chamber for containing liquids required for biochemical reactions; A lifting assembly is located on the outside of the housing. The lifting assembly is used to drive the reagent needle into or out of the storage chamber to extract the liquid. A cleaning assembly is used to provide cleaning fluid to the reagent needle located on the lifting assembly to clean the outer wall of the reagent needle; A purging assembly is used to purge air onto the reagent needle and the storage chamber to purge waste liquid on the reagent needle and in the storage chamber to the bottom of the storage chamber; as well as A drainage assembly is used to collect the waste liquid at the bottom of the storage chamber and discharge the liquid out of the storage chamber.

2. The biochemical reaction liquid storage module as described in claim 1, characterized in that, The lifting assembly includes: a lifting drive mechanism disposed on the outside of the housing, a mounting plate disposed on the lifting drive mechanism, and a plurality of mounting blocks disposed on the mounting plate. The mounting block includes a first mounting hole for mounting the reagent needle. The lifting drive mechanism is used to drive the mounting plate to move in a direction toward or away from the storage chamber, so that the reagent needle mounted in the first mounting hole extends into or out of the storage chamber.

3. The biochemical reaction liquid storage module as described in claim 2, characterized in that, The cleaning assembly includes a cleaning pipeline, and the mounting block further includes a second mounting hole disposed around and communicating with the first mounting hole. One end of the cleaning pipeline is installed in the second mounting hole, and the cleaning pipeline is used to provide cleaning fluid to the reagent needle located in the first mounting hole.

4. The biochemical reaction liquid storage module as described in claim 1, characterized in that, It also includes a reagent needle fixing assembly, which includes: a fixing plate disposed on the side of the storage compartment near the lifting assembly, and a plurality of hollow fixing sleeves disposed between the fixing plate and the housing, wherein the reagent needle located on the lifting assembly can pass through the housing, the fixing sleeves and the fixing plate to extend into the storage compartment.

5. The biochemical reaction liquid storage module as described in claim 4, characterized in that, The purging assembly includes an air blowing pipe, which includes multiple air blowing branches, one end of which is connected to a corresponding fixed sleeve.

6. The biochemical reaction liquid storage module as described in claim 1, characterized in that, The drainage assembly includes: a collection plate disposed at the bottom of the storage compartment, a drainage hole disposed at one end of the collection plate, and a drainage pipe communicating with the drainage hole. The collection plate includes a bottom plate near the bottom wall of the housing. The bottom plate includes a first end and a second end disposed opposite to each other. The distance between the bottom plate and the bottom wall of the housing decreases sequentially from the first end to the second end, so that the bottom plate is inclined. The drainage hole is disposed at the second end.

7. The biochemical reaction liquid storage module as described in claim 6, characterized in that, The drainage assembly also includes a baffle at the second end, the baffle and the bottom plate forming a collection groove, and an obtuse angle between the baffle and the bottom plate.

8. The biochemical reaction liquid storage module as described in claim 6, characterized in that, The flow collecting plate also includes a top plate disposed opposite to the bottom plate and a side plate connecting the top plate and the bottom plate. The top plate, the bottom plate and the side plate form a flow collecting cavity. The top plate is provided with an opening, and the storage compartment communicates with the flow collecting cavity through the opening.

9. The biochemical reaction liquid storage module as described in claim 1, characterized in that, It also includes a temperature control component, which is used to regulate the temperature inside the storage compartment.

10. A biochemical reaction system, characterized in that, include: A biochemical reaction platform and a biochemical reaction solution storage module as described in any one of claims 1 to 9, wherein the reagent storage module is used to provide biochemical reaction solution to the biochemical reaction platform through the reagent needle.

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