A method for preparing a soothing composition for repairing and uses thereof

Abstract

The application discloses a preparation method of a soothing repair composition and application thereof. The application adopts a step-by-step combined extraction process for extraction in view of the poor solubility of different components in three extracts, that is, the radix Stephaniae tetrandrae is subjected to composite enzymolysis and then subjected to microwave-assisted extraction, the citrus peel is subjected to low-temperature fermentation and then subjected to subcritical water extraction, and the leaf of agastache rugosus is subjected to supercritical CO2 extraction and then subjected to ethanol gradient elution. After the three extracts obtained through the process are compounded, the three extracts have excellent skin barrier repair, moisturizing and skin soothing effects and are safe and non-irritating, and can be used for skin after medical beauty, and skin with seasonal discomfort.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a preparation method of a soothing and repairing composition and application thereof. BACKGROUND

[0002] With the improvement of people's living standards and the enhancement of skin care consciousness, there is an increasing demand for skin care products with skin barrier repair, moisturizing and soothing effects. Especially after medical beauty, the skin and the skin prone to discomfort during the transition season need effective repair to restore the skin barrier function.

[0003] In the prior art, there are many compositions for skin repair, among which there are products containing plant extracts. Radix Stephaniae Tetrandrae, citrus fruit peel and Herba Pogostemonis are common plant raw materials, and their extracts have certain biological activity. However, the current extraction process of these extracts mostly adopts the conventional solvent extraction method, which is difficult to fully extract the effective components therein, and the synergistic effect between different extracts is not fully exerted, resulting in unsatisfactory skin barrier repair, moisturizing and soothing effects of the composition.

[0004] CN111870568B discloses an anti-allergic and itching-relieving plant composition, which is characterized by containing the following ingredients prepared from the following raw materials by weight: Radix Stephaniae Tetrandrae 1-8 parts, Ilex latifolia 0-6 parts, Scutellaria baicalensis 1-6 parts, Polygonum cuspidatum 1-6 parts, Glycyrrhiza glabra 1-6 parts, Camellia sinensis 0.5-6 parts, Sevenstar sword 0-6 parts, Mutisya white layer 0-6 parts, Mother chrysanthemum 0.5-6 parts, Centella asiatica 0.5-6 parts, Rosemary 0.5-6 parts; the powder of the raw materials is mixed to extract the bioactive substances, including: grinding and mixing Radix Stephaniae Tetrandrae, optional Ilex latifolia, Scutellaria baicalensis, Polygonum cuspidatum and Glycyrrhiza glabra, and then performing 40-80% ethanol immersion extraction; grinding and mixing Camellia sinensis, optional Sevenstar sword, optional Mutisya white layer, mother chrysanthemum, Centella asiatica and Rosemary, and then performing volatile oil extraction. The composition contains multiple plant raw materials, the synergistic effect between the components is not clear, and a 40-80% ethanol immersion extraction process is adopted, which does not have the function of repairing the skin barrier.

[0005] CN118649114A discloses an olive soothing compound with soothing synergistic effect, a preparation method and application thereof. The olive soothing compound comprises: olive leaf extract powder 5-10 parts, citrus fruit extract liquid 45-55 parts, polyhydric alcohol 25-45 parts, and water 0-20 parts. It has good soothing effect and can be used for the preparation of sensitive skin repair products. The preparation method of the citrus fruit extract liquid adopts water extraction of citrus fruit peel, and the yield of effective components is not high, and it does not have the function of repairing the skin barrier.

[0006] Therefore, an optimized extraction process for three plant raw materials, i.e., Stephania tetrandra S. Moore, citrus peel and pachystachys juttah, is developed to improve the efficacy of effective components and enhance the synergistic effect among the extracts, so as to obtain a composition with excellent skin barrier repairing, moisturizing and skin soothing effects and safety and no irritation, which is suitable for various damaged skin such as medical beauty after, seasonal sensitivity and the like. SUMMARY

[0007] The present application aims to overcome the shortcomings of the prior art and provide a preparation method of a soothing and repairing composition and application thereof.

[0008] To achieve the above-mentioned object, the technical scheme adopted by the present application is as follows:

[0009] In a first aspect, the present application provides a preparation method of a soothing and repairing composition, which comprises the following steps:

[0010] (1) Preparation of Stephania tetrandra S. Moore extract: take Stephania tetrandra S. Moore powder and deionized water at a mass ratio of 1:(8-12), mix and stir uniformly, add a composite enzyme solution, and carry out enzymolysis at 35-40℃ and pH 4.5-5.0 for 1-2h; after the enzymolysis is completed, microwave-assisted extraction is carried out at a microwave power of 240-360W for 20-25min; after the extraction, filtration, concentration and drying are carried out, the Stephania tetrandra S. Moore extract is obtained;

[0011] (2) Preparation of citrus peel extract: take citrus peel powder and deionized water at a mass ratio of 1:(6-10), mix and stir uniformly, inoculate with Lactobacillus plantarum, and carry out fermentation at 30-35℃ for 8-12h; after the fermentation is completed, the fermentation mixture is transferred to an extraction kettle, deionized water is added at a mass ratio of 1:(10-20) to the citrus peel powder, and subcritical water extraction is carried out at an extraction temperature of 120-130℃ and a pressure of 3-4MPa; after the extraction, filtration, concentration and drying are carried out, the citrus peel extract is obtained;

[0012] (3) Preparation of pachystachys juttah leaf extract: take pachystachys juttah leaf raw material, crush it, and add it to an extraction kettle; add 65-75% ethanol aqueous solution as a entraining agent at a speed of 0.05-0.15L / h, and carry out supercritical CO2 extraction to obtain volatile oil; take the residue after the extraction, use 50%-70% ethanol aqueous solution to carry out reflux extraction at 60-70℃, and carry out filtration, concentration and drying to obtain non-volatile component extract; mix the volatile oil and the non-volatile component extract to obtain the pachystachys juttah leaf extract;

[0013] (4) Mix the Stephania tetrandra S. Moore extract obtained in step (1), the citrus peel extract obtained in step (2) and the pachystachys juttah leaf extract obtained in step (3) uniformly at a mass ratio of 1:(0.5-1.5):(0.5-1.5) to obtain the soothing and repairing composition.

[0014] Preferably, in step (1), the complex enzyme is xylanase and acid protease, and the mass ratio of xylanase and acid protease is 1:(0.5-1), and the amount of the complex enzyme solution is 0.5-1wt% of the mass of the powder of Stephania tetrandra S. Moore.

[0015] Preferably, in step (2), the inoculation amount of the Lactobacillus plantarum is 0.4-0.8wt% of the mass of the citrus peel powder.

[0016] Preferably, in step (3), the supercritical CO2 extraction pressure is 25-30MPa, the extraction temperature is 40-50℃, and the extraction time is 1-1.5h.

[0017] Preferably, in step (4), the mass ratio of the Stephania tetrandra S. Moore extract, the citrus peel extract, and the Pogostemonis Herba leaf extract is 1:1:1.

[0018] In a second aspect, the present application provides a soothing and repairing composition prepared by the above method.

[0019] In a third aspect, the present application provides an application of the soothing and repairing composition in the preparation of a skin barrier repairing skin care product, and the amount of the soothing and repairing composition in the skin care product is 0.5-5wt%, and the skin care product includes any one of cream, emulsion, and essence.

[0020] Compared with the prior art, the present application has the following beneficial effects:

[0021] (1) A step-by-step combination extraction process is adopted, and extraction methods are designed according to the characteristics of Stephania tetrandra S. Moore, citrus peel, and Pogostemonis Herba leaf. The combination of complex enzyme hydrolysis (xylanase and acid protease) and microwave-assisted extraction of Stephania tetrandra S. Moore improves the yield of effective components; the combination of low-temperature fermentation (Lactobacillus plantarum) and subcritical water extraction of citrus peel can decompose allergens and optimize the dissolution of components; and the combination of supercritical CO2 extraction and ethanol gradient elution of Pogostemonis Herba leaf realizes the effective extraction of volatile oil and non-volatile components.

[0022] (2) After the three extracts of Stephania tetrandra S. Moore extract, citrus peel extract, and Pogostemonis Herba leaf extract are compounded in a specific ratio, they exhibit a synergistic effect in repairing skin barrier, moisturizing, and soothing skin, and are safe and non-irritating. The anti-inflammatory effect of the Stephania tetrandra S. Moore extract, the promotion of the repair of stratum corneum by the citrus peel extract, and the regulation of the microecology by the Pogostemonis Herba leaf extract form a multi-dimensional synergistic mechanism, and the effect is better than that of a traditional solvent extraction process mixed extract.

[0023] (3) Through the innovative process, the irritating impurities are effectively removed, the skin patch test proves that there is no irritation, and the soothing composition is suitable for various damaged skin such as post-medical beauty, seasonal sensitivity, etc. The soothing composition can be applied to various skin care products such as creams, lotions, serums, etc., to meet the needs of different groups of people, and has a wide market application prospect. DETAILED DESCRIPTION

[0024] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples. The materials, reagents and the like used in the examples can be obtained from commercial channels unless otherwise specified.

[0025] The microbial sources of the present application are xylanase (enzyme activity >100000U / g) and acid protease (enzyme activity 50000U / g), which are purchased from Shanghai Yuan Ye Biological Technology Co., Ltd. Lactobacillus plantarum is preserved in China Industrial Microbial Culture Collection Center with the preservation number CICC20242.

[0026] Example 1

[0027] A preparation method of a soothing composition, comprising the following steps:

[0028] (1) Preparation of Stephania tetrandra extract: take Stephania tetrandra powder and deionized water in a mass ratio of 1:8, mix and stir uniformly, add a composite enzyme solution (the composite enzyme is xylanase and acid protease, the mass ratio is 1:0.5, and the amount of the composite enzyme solution is 0.5wt% of the mass of the Stephania tetrandra powder), and perform enzymolysis at 35℃ and pH 4.5 for 2h. After the enzymolysis is completed, microwave-assisted extraction is performed, the microwave power is 240W, and the extraction time is 20min. After extraction, filtration, concentration and drying are performed to obtain the Stephania tetrandra extract.

[0029] (2) Preparation of citrus peel extract: take citrus peel powder and deionized water in a mass ratio of 1:6, mix and stir uniformly, inoculate Lactobacillus plantarum, the inoculation amount is 0.4wt% of the mass of the citrus peel powder, and perform fermentation at 30℃ for 12h. After the fermentation is completed, the fermentation mixture is transferred to an extraction kettle, deionized water is added according to the mass ratio of 1:10 of the citrus peel powder and deionized water, and then subcritical water extraction is performed, the extraction temperature is 120℃, and the pressure is 3MPa. After extraction, filtration, concentration and drying are performed to obtain the citrus peel extract.

[0030] (3) Preparation of Pogostemonis Folium extract: Take Pogostemonis Folium raw material, crush it and add it into an extraction kettle. Add 65% ethanol water solution as an entraining agent at a speed of 0.05 L / h. Perform supercritical CO2 extraction at an extraction pressure of 25 MPa and a temperature of 40°C for 1.5 h to obtain volatile oil. Take the residue after extraction, use 50% ethanol water solution to perform reflux extraction at 60°C, filter, concentrate and dry to obtain non-volatile component extract. Mix the volatile oil and the non-volatile component extract to obtain Pogostemonis Folium extract.

[0031] (4) Mix the powder of Stephania tetrandra S. Moore extract obtained in step (1), the citrus peel extract obtained in step (2) and the Pogostemonis Folium extract obtained in step (3) in a mass ratio of 1:0.5:0.5 to obtain a soothing composition.

[0032] Example 2

[0033] A method for preparing a soothing composition comprises the following steps:

[0034] (1) Preparation of Stephania tetrandra S. Moore extract: Take powder of Stephania tetrandra S. Moore and deionized water in a mass ratio of 1:10, mix and stir them uniformly, add a composite enzyme solution (the composite enzyme is xylanase and acid protease, the mass ratio of which is 1:0.8, and the amount of the composite enzyme solution is 0.8wt% of the mass of the powder of Stephania tetrandra S. Moore), and perform enzymolysis at 37°C and pH 4.7 for 1.5 h. After the enzymolysis is completed, perform microwave-assisted extraction, the microwave power is 300 W, and the extraction time is 22 min. After the extraction, filter, concentrate and dry to obtain the Stephania tetrandra S. Moore extract.

[0035] (2) Preparation of citrus peel extract: Take citrus peel powder and deionized water in a mass ratio of 1:8, mix and stir them uniformly, inoculate Lactobacillus plantarum, the inoculation amount is 0.6wt% of the mass of the citrus peel powder, and perform fermentation at 33°C for 10 h. After the fermentation is completed, transfer the fermentation mixture to an extraction kettle, add deionized water in a mass ratio of 1:15 of the citrus peel powder to the deionized water, and perform subcritical water extraction, the extraction temperature is 125°C and the pressure is 3.5 MPa. After the extraction, filter, concentrate and dry to obtain the citrus peel extract.

[0036] (3) Preparation of Pogostemonis Folium extract: Take Pogostemonis Folium raw material, crush it and add it into an extraction kettle. Add 70% ethanol water solution as an entraining agent at a speed of 0.1 L / h. Perform supercritical CO2 extraction at an extraction pressure of 27 MPa and a temperature of 45°C for 1.2 h to obtain volatile oil. Take the residue after extraction, use 60% ethanol water solution to perform reflux extraction at 65°C, filter, concentrate and dry to obtain non-volatile component extract. Mix the volatile oil and the non-volatile component extract to obtain Pogostemonis Folium extract.

[0037] (4) The powder aconite extract obtained in step (1), the citrus peel extract obtained in step (2), and the pachouli leaf extract obtained in step (3) are mixed uniformly at a mass ratio of 1:1:1 to obtain a soothing repair composition.

[0038] Example 3

[0039] A method for preparing a soothing repair composition comprises the following steps:

[0040] (1) Preparation of powder aconite extract: powder aconite powder and deionized water are taken at a mass ratio of 1:12, mixed and stirred uniformly, a composite enzyme solution (the composite enzyme is xylanase and acid protease, the mass ratio of which is 1:1, and the amount of the composite enzyme solution is 1.0wt% of the mass of the powder aconite powder) is added, and enzymolysis is carried out at 40℃ and pH 5.0 for 1h. After the enzymolysis is completed, microwave-assisted extraction is carried out at a microwave power of 360W for 25min. After the extraction, filtration, concentration, and drying are carried out to obtain the powder aconite extract.

[0041] (2) Preparation of citrus peel extract: citrus peel powder and deionized water are taken at a mass ratio of 1:10, mixed and stirred uniformly, and Lactobacillus plantarum is inoculated at an inoculation amount of 0.8wt% of the mass of the citrus peel powder, and fermentation is carried out at 35℃ for 8h. After the fermentation is completed, the fermentation mixture is transferred to an extraction kettle, deionized water is added to make the mass ratio of the citrus peel powder to the deionized water 1:20, and subcritical water extraction is carried out at an extraction temperature of 130℃ and a pressure of 4MPa. After the extraction, filtration, concentration, and drying are carried out to obtain the citrus peel extract.

[0042] (3) Preparation of pachouli leaf extract: pachouli leaf raw materials are taken, crushed, and added to an extraction kettle. A 75wt% ethanol aqueous solution is added as a carrier at a speed of 0.15L / h to carry out supercritical CO2 extraction at an extraction pressure of 30MPa and a temperature of 50℃ for 1h to obtain volatile oil. The residue after the extraction is taken, a 70wt% ethanol aqueous solution is used to carry out reflux extraction at 70℃, and filtration, concentration, and drying are carried out to obtain a non-volatile component extract. The volatile oil and the non-volatile component extract are mixed to obtain the pachouli leaf extract.

[0043] (4) The powder aconite extract obtained in step (1), the citrus peel extract obtained in step (2), and the pachouli leaf extract obtained in step (3) are mixed uniformly at a mass ratio of 1:1.5:1.5 to obtain a soothing repair composition.

[0044] Comparative Example 1

[0045] A method for preparing a soothing repair composition, which is different from example 2 in that only contains the powder aconite extract, the preparation method of the powder aconite extract is the same as example 2, the mass of the citrus peel extract and the pachouli leaf extract in the composition is replaced by an equal amount of the powder aconite extract.

[0046] Comparative example 2

[0047] A method for preparing a soothing repair composition, which is different from example 2 in that only contains the citrus peel extract, the preparation method of the citrus peel extract is the same as example 2, the mass of the powder aconite extract and the pachouli leaf extract in the composition is replaced by an equal amount of the citrus peel extract.

[0048] Comparative example 3

[0049] A method for preparing a soothing repair composition, which is different from example 2 in that only contains the pachouli leaf extract, the preparation method of the pachouli leaf extract is the same as example 2, the mass of the powder aconite extract and the citrus peel extract in the composition is replaced by an equal amount of the pachouli leaf extract.

[0050] Comparative example 4

[0051] A method for preparing a soothing repair composition, which is different from example 2 in that only contains the powder aconite extract and the citrus peel extract, the preparation method of the powder aconite extract and the citrus peel extract is the same as example 2, the mass of the pachouli leaf extract in the composition is replaced by an equal amount of the powder aconite extract and the citrus peel extract, so that the mass ratio of the powder aconite extract and the citrus peel extract in the composition is 1:1.

[0052] Comparative example 5

[0053] A method for preparing a soothing repair composition, which is different from example 2 in that only contains the powder aconite extract and the pachouli leaf extract, the preparation method of the powder aconite extract and the pachouli leaf extract is the same as example 2, the mass of the citrus peel extract in the composition is replaced by an equal amount of the powder aconite extract and the pachouli leaf extract, so that the mass ratio of the powder aconite extract and the pachouli leaf extract in the composition is 1:1.

[0054] Comparative example 6

[0055] A method for preparing a soothing repair composition, which is different from example 2 in that only contains the citrus peel extract and the pachouli leaf extract, the preparation method of the citrus peel extract and the pachouli leaf extract is the same as example 2, the mass of the powder aconite extract in the composition is replaced by an equal amount of the citrus peel extract and the pachouli leaf extract, so that the mass ratio of the citrus peel extract and the pachouli leaf extract in the composition is 1:1.

[0056] Comparative example 7

[0057] A method for preparing a soothing composition, wherein the ethanol reflux extraction is used for extracting the extract of radix Stephaniae tetrandrae (taking the powder of radix Stephaniae tetrandrae and 70% ethanol aqueous solution with a mass concentration of 1:10, refluxing at 80°C for 2h, filtering, concentrating and drying); the water extraction is used for extracting the extract of citrus peel (taking the powder of citrus peel and deionized water with a mass ratio of 1:8, extracting at 90°C for 1.5h, filtering, concentrating and drying); and the ethanol extraction is used for extracting the extract of leaves of pachystachys juttah (taking the leaves of pachystachys juttah, crushing, and then refluxing at 65°C for 1.5h with 60% ethanol aqueous solution, filtering, concentrating and drying). The three kinds of extracts are mixed uniformly with a mass ratio of 1:1:1 to obtain the composition.

[0058] Test Example 1: Skin irritation test

[0059] According to the Human Patch Test in the Cosmetic Safety Technical Specification (2015 Edition), the compositions prepared in Examples 1-3 are selected as experimental products, 30 healthy subjects are selected, half of them are male and half of them are female, and the closed patch test is carried out on the inner side of the forearm and behind the ear of the subjects, and after 24 hours, the patch tester is removed, and 30 minutes, 24 hours and 48 hours after removal, it is observed whether there are erythema, edema, papules, blisters and other irritating reactions on the skin.

[0060] The experimental results show that the patch test results of the 30 subjects are all negative, and there are no erythema, edema, papules, blisters and other irritating reactions. It is thus shown that the compositions prepared in Examples 1-3 have no obvious irritation and sensitization to the human body, and have good safety performance.

[0061] Test Example 2: TEWL test

[0062] A trans-epidermal water loss tester is used, 30 subjects are selected and randomly divided into 10 groups, 3 people in each group, and the compositions prepared in Examples 1-3 and Comparative Examples 1-7 (prepared into a solution with a concentration of 5wt% by using deionized water) are respectively used on the skin surface under the same environment, once a day in the morning and evening. The TEWL values of the same skin parts of the subjects are measured before use, after 14 days and 28 days of use, each subject is tested 3 times, the average value is taken, the average reduction rate of TEWL after 14 days and 28 days of use is calculated, the TEWL reduction rate (%) = (TEWL value before use-TEWL value after n days of use) / TEWL value before use x 100%, and the average reduction rate of TEWL (%) = the sum of the TEWL value reduction rates of each group / the number of subjects in the group, and the results are shown in Table 1.

[0063] Table 1: TEWL test results

[0064]

[0065] The TEWL value reflects the skin barrier integrity, the higher the TEWL reduction rate, the better the skin barrier repair effect. When the subjects continuously use the compositions of Examples 1-3 above, the skin TEWL value gradually decreases, the average TEWL reduction rate increases, and the effect of Examples 1-3 is obviously better than that of Comparative Examples 1-7, indicating that the composition prepared by the application has good water retention effect, which is closely related to the synergistic repair of skin barrier by the three extracts of tetrandrine extract, citrus peel extract and pachouli leaf extract. Tetrandrine in tetrandrine extract can inhibit the release of inflammatory factors, reduce the damage of skin inflammation to the barrier, and also can improve the skin microcirculation and promote water transport; hesperidin in citrus peel extract can promote the proliferation of keratinocytes and enhance the stability of stratum corneum structure, and the polysaccharide component in citrus peel extract has strong water absorption and can lock water; pachouli ketone in pachouli leaf extract can regulate skin microecology and reduce the invasion of harmful bacteria to the barrier, and pachouli leaf extract can also enhance the synthesis of ceramide in stratum corneum, which is an important lipid to maintain the hydration state of stratum corneum. The three of them synergistically repair the skin barrier, thereby effectively reducing the TEWL value and improving the water retention of the skin. The skin barrier repair effect of Comparative Examples 1-6 lacking one or two of the extracts is obviously decreased, and the TEWL value of Comparative Example 7 using traditional solvent extraction process is reduced to a smaller extent due to the low yield of effective components.

[0066] Test Example 3: Stratum Corneum Water Content Test

[0067] A skin moisture tester was used to select 30 subjects, which were randomly divided into 10 groups, 3 people in each group, and the compositions prepared by Examples 1-3 and Comparative Examples 1-7 (prepared into a solution with a concentration of 5wt% by using deionized water) were respectively used on the skin surface under the same environment, once a day in the morning and evening. The stratum corneum water content of the same skin part of the subjects was measured before use, after 14 days and 28 days of use, each subject was tested 3 times, and the average value was taken to calculate the average improvement rate of stratum corneum water content after 14 days and 28 days of use, the water content improvement rate (%) = (water content after n days of use - water content before use) / water content before use x 100%, and the average water content improvement rate (%) = the sum of water content improvement rate in each group / the number of subjects in the group. The results are shown in Table 2.

[0068] Table 2: Results of Stratum Corneum Water Content Test

[0069]

[0070]

[0071] The stratum corneum water content reflects the skin moisturizing capacity, and the higher the water content improvement rate, the better the moisturizing effect. When the subjects continuously use the compositions of Examples 1-3 above, the skin water content improvement rate gradually increases, and the effect of Examples 1-3 is obviously better than that of Comparative Examples 1-7, indicating that the composition prepared by the application has good moisturizing and water supplementing effect, which benefits from the synergistic moisturizing and skin barrier repair mechanism of the three extracts. The extract of radix Stephaniae tetrandrae can improve the skin microcirculation and promote water transport; the polysaccharide component in the citrus peel extract has strong water absorption and can lock water; the pachouli leaf extract can enhance the synthesis of ceramide in the stratum corneum, and ceramide is an important lipid for maintaining the hydration state of the stratum corneum, and the three of them synergistically improve the water content of the stratum corneum. The traditional solvent extraction process group of Comparative Example 7 has low yield of effective components, insufficient water retention related components, and low improvement rate compared with Examples.

[0072] Test Example 4: Skin Redness Improvement Test

[0073] A skin colorimeter was used to select 30 subjects, who were randomly divided into 10 groups, 3 people in each group, and used the compositions prepared by Examples 1-3 and Comparative Examples 1-7 (respectively prepared into a solution with a concentration of 5wt% by using deionized water) on the skin surface in the same environment, once a day in the morning and evening. The a value of the redness part of the face of the subjects was measured before use, after 14 days and 28 days of use, each subject was tested 3 times, and the average value was taken to calculate the average improvement rate of skin redness after 14 days and 28 days of use, the redness improvement rate (%) = (a value before use-a value after n days of use) / a value before use x 100%, the average improvement rate of redness (%) = the sum of the redness improvement rate of each group / the number of subjects in the group, and the results are shown in Table 3.

[0074] Table 3: Results of Skin Redness Improvement Test

[0075]

[0076] The decrease in the a value and the increase in the redness improvement rate mean that the inflammation and sensitive state of the skin are improved. When the subjects continuously use the compositions of Examples 1-3 described above, the a value improvement rate is significantly improved, the skin redness is significantly improved, and a significant anti-inflammatory soothing effect is exhibited. This can be because the tetrandrine in the Stephania tetrandra extract can inhibit the release of inflammatory factors, thereby reducing skin redness from the source; the hesperidin in the citrus peel extract has strong antioxidant effects and can scavenge free radicals, thereby reducing inflammation caused by oxidative stress; and the volatile oil components in the Agastache rugosa leaf extract can soothe skin nerve endings and reduce skin sensitivity. The three extracts synergistically work together to reduce skin redness from multiple pathways, such as inhibiting the release of inflammatory mediators, antioxidant effects, and soothing nerves. The comparative examples 1-6, which lack one or two of the extracts, can only play an anti-inflammatory soothing role in some aspects and are less effective than Examples 1-3. Comparative example 7 has a low yield of effective components due to the use of a traditional extraction process, and the skin redness improvement effect is not good.

[0077] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A method of making a healing balm composition, characterized in that, The method comprises the following steps: (1) Preparation of the powder of Stephania tetrandra S. Moore extract: take the powder of Stephania tetrandra S. Moore and deionized water at a mass ratio of 1:(8-12), mix and stir uniformly, add a complex enzyme solution, and carry out enzymolysis at 35-40 DEG C and pH 4.5-5.0 for 1-2 h; after the enzymolysis is completed, microwave-assisted extraction is carried out at a microwave power of 240-360 W for 20-25 min; after the extraction, filtration, concentration and drying are carried out, the powder of Stephania tetrandra S. Moore extract is obtained; the complex enzyme is xylanase and acid protease, and the mass ratio of xylanase to acid protease is 1:(0.5-1); the amount of the complex enzyme solution is 0.5-1 wt% of the mass of the powder of Stephania tetrandra S. Moore; (2) Preparation of the citrus peel extract: take the citrus peel powder and deionized water at a mass ratio of 1:(6-10), mix and stir uniformly, inoculate lactobacillus plantarum, and carry out fermentation at 30-35 DEG C for 8-12 h; after the fermentation is completed, the fermented mixture is transferred to an extraction kettle, deionized water is added at a mass ratio of 1:(10-20) of the citrus peel powder to the deionized water, and subcritical water extraction is carried out at an extraction temperature of 120-130 DEG C and a pressure of 3-4 MPa; after the extraction, filtration, concentration and drying are carried out, the citrus peel extract is obtained; (3) Preparation of the extract of the leaves of Pogostemonis Herba: take the leaves of Pogostemonis Herba as raw materials, crush them, and add them to an extraction kettle; add an ethanol aqueous solution with a mass concentration of 65-75% as a carrier at a speed of 0.05-0.15 L / h, and carry out supercritical CO2 extraction to obtain volatile oil; take the residue after the extraction, use an ethanol aqueous solution with a mass concentration of 50-70% to carry out reflux extraction at 60-70 DEG C, and carry out filtration, concentration and drying to obtain a non-volatile component extract; mix the volatile oil and the non-volatile component extract to obtain the extract of the leaves of Pogostemonis Herba; (4) Mix the powder of Stephania tetrandra S. Moore extract obtained in step (1), the citrus peel extract obtained in step (2) and the extract of the leaves of Pogostemonis Herba obtained in step (3) uniformly at a mass ratio of 1:(0.5-1.5):(0.5-1.5) to obtain a soothing and repairing composition.

2. The production method according to claim 1, wherein In step (2), the inoculation amount of the lactobacillus plantarum is 0.4-0.8 wt% of the mass of the citrus peel powder.

3. The production method according to claim 1, wherein In step (3), the supercritical CO2 extraction pressure is 25-30 MPa, the extraction temperature is 40-50 DEG C, and the extraction time is 1-1.5 h.

4. The production method according to claim 1, wherein In step (4), the mass ratio of the powder of Stephania tetrandra S. Moore extract, the citrus peel extract and the extract of the leaves of Pogostemonis Herba is 1:1:

1.

5. A soothing and repairing composition prepared by the method of any one of claims 1-4.

6. Use of a repair soothing composition according to claim 5, characterized in that, The amount of the soothing and repairing composition in the skin care product is 0.5-5 wt%.

7. Use according to claim 6, wherein The skin care product comprises any one of a cream, a lotion and an essence.

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