Device for detecting five-classification blood cells and MxA to identify viral infection

Abstract

The invention discloses a device for detecting five-classification blood cells and MxA to identify viral infection. The device comprises a DIFF reaction detection system, an MxA immunofluorescence detection system, a sample collection system, a first DIFF reagent pipeline and a second DIFF reagent pipeline, a collected sample is conveyed to the DIFF reaction detection system through the sample collection system, a first DIFF reagent in a first DIFF reagent pool is guided to the DIFF reaction detection system through the first DIFF reagent pipeline, and a second DIFF reagent in a second DIFF reagent pool is guided to the second DIFF reaction detection system through the second DIFF reagent pipeline. A second DIFF reagent pipeline is used for guiding a second DIFF reagent in a second DIFF reagent pool to a DIFF reaction detection system, so that DIFF reaction detection is completed in the DIFF reaction detection system, a collected sample is conveyed to an MxA immunofluorescence detection system through a sample collection system, a first pushing unit is used for pushing an MxA reagent card strip on the uppermost layer of an MxA reagent card strip stacking unit to a conveying unit, and a second pushing unit is used for pushing a second DIFF reagent card strip on the uppermost layer of the MxA reagent card strip stacking unit to the conveying unit. The conveying unit is used for conveying the MxA reagent card strip to sequentially pass through the photometry and incubation unit and the reading unit, so that the device can quickly complete the detection of the individual viral infection and the degree thereof.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a device for detecting five-classified blood cells and MxA to identify viral infection. BACKGROUND

[0002] In the related art, the five-classified blood cell detection and the immunofluorescence detection of MxA are usually performed by two independent devices, and the user needs to manually move the MxA reagent card strip. Not only the number of patient detections is large, but also the detection efficiency is relatively low. SUMMARY

[0003] The embodiments of the present application provide a device for detecting five-classified blood cells and MxA to identify viral infection, which can simultaneously perform the five-classified blood cell detection and the immunofluorescence detection of MxA, and can take into account the detection efficiency of immunofluorescence.

[0004] The embodiments of the present application provide a device for detecting five-classified blood cells and MxA to identify viral infection, comprising: a DIFF reaction detection system; an MxA immunofluorescence detection system, the MxA immunofluorescence detection system comprising a first pushing unit, an MxA reagent card strip stacking unit, a conveying unit, a light measurement and incubation unit, and a reading unit, the MxA reagent card strip stacking unit being used to stack MxA reagent card strips, the first pushing unit being used to push the MxA reagent card strip on the uppermost layer of the MxA reagent card strip stacking unit to the conveying unit, the conveying unit being used to convey the MxA reagent card strip to sequentially pass through the light measurement and incubation unit and the reading unit; a sample collection system, used to convey the collected sample to the DIFF reaction detection system or the MxA immunofluorescence detection system; a first DIFF reagent pipeline, used to communicate with a first DIFF reagent pool and the DIFF reaction detection system, so as to guide the first DIFF reagent in the first DIFF reagent pool to the DIFF reaction detection system; a second DIFF reagent pipeline, used to communicate with a second DIFF reagent pool and the DIFF reaction detection system, so as to guide the second DIFF reagent in the second DIFF reagent pool to the DIFF reaction detection system.

[0005] In some embodiments, the MxA reagent card strip stacking unit for detecting MxA comprises: a stand, formed with a stock bin for accommodating MxA reagent card strips, the stock bin forming a discharge port on the upper surface of the stand; a bottom plate, provided at the bottom of the stand, the bottom plate being provided with a through hole in communication with the stock bin; A top mechanism is arranged below the bottom plate, and the top mechanism comprises a top member movably arranged in a vertical direction to extend into the hopper through the through hole and to push the uppermost MxA reagent card strip out of the hopper. The first pushing unit is configured to push the uppermost MxA reagent card strip outside the hopper to the conveying unit.

[0006] In some embodiments, the MxA reagent card strip stacking unit comprises: a mounting plate; a plurality of vertical supports distributed on the mounting plate in a circumferential direction of the mounting plate; a plurality of bottom plates arranged at the bottom of the vertical supports one by one; and a rotating mechanism arranged to rotate with the mounting plate to drive the mounting plate to rotate about an axis of the mounting plate.

[0007] In some embodiments, the device for detecting five-class blood cells and MxA to identify viral infection further comprises: an HGB detection system comprising a WBC pool, an RBC pool, and a negative pressure chamber in communication with the WBC pool and the RBC pool, respectively; and a WBC reagent pipeline in communication with a WBC reagent pool and the WBC pool to guide the WBC reagent in the WBC reagent pool to the WBC pool; The sample collection system is further configured to deliver the collected sample to the WBC pool and the RBC pool.

[0008] In some embodiments, the device for detecting five-class blood cells and MxA to identify viral infection further comprises a waste liquid collection system in communication with the WBC pool, the RBC pool, the negative pressure chamber, and the DIFF reaction detection system, respectively.

[0009] In some embodiments, the device for detecting five-class blood cells and MxA to identify viral infection further comprises a dilution liquid delivery pipeline provided with a distribution three-way valve, the distribution three-way valve having one distribution inlet and two distribution outlets, the distribution inlet being in communication with a dilution liquid pool, and the two distribution outlets being in communication with the WBC pool and the RBC pool, respectively.

[0010] In some embodiments, the diluent delivery line is provided with a first three-way valve, one port of which is in communication with the diluent reservoir, and a first injection unit, the other two ports of which are in communication with the first injection unit and the distribution inlet, respectively, the first injection unit being configured to direct the diluent from the diluent reservoir into the distribution three-way valve.

[0011] In some embodiments, the sample collection system includes a second three-way valve, one port of which is in communication with the diluent reservoir, and a second injection unit, the other two ports of which are in communication with the second injection unit and the sample collection unit, respectively.

[0012] In some embodiments, the first DIFF reagent line is provided with a third three-way valve, one port of which is in communication with the first DIFF reagent reservoir, and a third injection unit, the other two ports of which are in communication with the third injection unit and the DIFF reaction detection system, respectively, the third injection unit being configured to direct the first DIFF reagent from the first DIFF reagent reservoir into the DIFF reaction detection system. and / or, the second DIFF reagent line is provided with a fourth three-way valve, one port of which is in communication with the second DIFF reagent reservoir, and a fourth injection unit, the other two ports of which are in communication with the fourth injection unit and the DIFF reaction detection system, respectively, the fourth injection unit being configured to direct the second DIFF reagent from the second DIFF reagent reservoir into the DIFF reaction detection system.

[0013] In some embodiments, the DIFF reaction detection system includes a DIFF reaction detection unit, a fifth injection unit, and a sixth injection unit, the DIFF reaction detection unit including a reaction zone and a detection zone, the fifth injection unit being in communication with the reaction zone, and the sixth injection unit being in communication with the detection zone, the fifth injection unit being configured to provide power to push the sample on the reaction zone into the detection zone, and the sixth injection unit being configured to provide sheath liquid into the detection zone.

[0014] The device for detecting five-class blood corpuscles and MxA to identify viral infection based on the embodiment of the application comprises a DIFF reaction detection system, an MxA immunofluorescence detection system, a sample collection system, a first DIFF reagent pipeline and a second DIFF reagent pipeline. The collected sample is transported to the DIFF reaction detection system through the sample collection system. The first DIFF reagent pipeline guides the first DIFF reagent in the first DIFF reagent pool to the DIFF reaction detection system. The second DIFF reagent pipeline guides the second DIFF reagent in the second DIFF reagent pool to the DIFF reaction detection system. Thus, the DIFF reaction detection is completed in the DIFF reaction detection system. The collected sample is transported to the MxA immunofluorescence detection system through the sample collection system. In the MxA immunofluorescence detection system, the first pushing unit pushes the MxA reagent card strip on the uppermost layer of the MxA reagent card strip stacking unit to the conveying unit. The conveying unit is used to transport the MxA reagent card strip to sequentially pass through the light measurement and incubation unit and the reading unit, so that the detection of the content of MxA is quickly completed. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0016] Figure 1 The structural schematic diagram of the device for detecting five-class blood corpuscles and MxA to identify viral infection provided by the embodiment of the present application is shown in the figure. Figure 2 The structural schematic diagram of the MxA reagent card strip stacking unit provided by the embodiment of the present application is shown in the figure. Figure 3 The structural schematic diagram of the MxA immunofluorescence detection system provided by the embodiment of the present application is shown in the figure.

[0017] Reference numerals in the figure: 100, DIFF reaction detection system; 110, DIFF reaction detection unit; 120, fifth injection unit; 130, sixth injection unit; 200, MxA immunofluorescence detection system; 210, first pushing unit; 220, MxA reagent card strip stacking unit; 221, stand; 221a, stock bin; 221b, discharge port; 222, bottom plate; 223, top feeding mechanism; 224, mounting plate; 225, rotating mechanism; 230, conveying unit; 240, light measurement and incubation unit; 250, reading unit; 300. Sample collection system; 310. Second three-way valve; 320. Second injection unit; 330. Sample collection unit; 400, First DIFF reagent tubing; 410, Third three-way valve; 420, Third injection unit; 500. Second DIFF reagent tubing; 510. Fourth three-way valve; 520. Fourth injection unit; 600. HGB detection system; 610. WBC pool; 620. RBC pool; 630. Negative pressure chamber; 700, WBC reagent tubing; 800. Waste liquid collection system; 900. Diluent delivery pipeline; 910. First three-way valve; 920. First injection unit; 930. Distribution three-way valve; 10. Diluent tank; 20. First DIFF reagent tank; 30. Second DIFF reagent tank; 40. WBC reagent tank. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0019] In existing technologies, the five-part differential hematology test and the immunofluorescence assay for MxA are usually performed using two separate devices, and the user also needs to manually move the MxA reagent strip. This results in more tests for patients and relatively low testing efficiency.

[0020] To resolve the above technical issues, please refer to Figures 1-2 This application proposes an apparatus for detecting five-part differential hematology cells and MxA to identify viral infections, comprising a DIFF (Differential Leukocyte Count) reaction detection system 100, an MxA immunofluorescence detection system 200, a sample collection system 300, a first DIFF reagent line 400, and a second DIFF reagent line 500.

[0021] The DIFF reaction detection system 100 uses flow cytometry or cytochemical staining techniques to perform a five-part differential count of white blood cells (neutrophils, lymphocytes, monocytes, eosinophils, and basophils) to assess infection type, immune status, and risk of blood disorders.

[0022] The MxA immunofluorescence detection system 200 for detecting MxA (Myxovirus resistance protein A) includes a first pushing unit 210, an MxA reagent strip stacking unit 220, a conveying unit 230, a photometric and incubation unit 240, and a reading unit 250. The MxA reagent strip stacking unit 220 is used to stack MxA reagent strips. The first pushing unit 210 is used to push the topmost MxA reagent strip of the MxA reagent strip stacking unit 220 to the conveying unit 230. The conveying unit 230 is used to transport the MxA reagent strips sequentially through the photometric and incubation unit 240 and the reading unit 250. The MxA reagent strip stacking unit 220 can stack multiple MxA reagent strips together, pre-loaded with a large number of MxA reagent strips, and stack them vertically in sequence. The top layer of MxA reagent strips is in a standby state and can be pushed at any time. The first pushing unit 210 pushes the top layer of MxA reagent strips horizontally to the starting position of the conveying unit 230 via a robotic arm or linear slide, at which point the MxA reagent strips enter the starting point of the detection process.

[0023] The conveying unit 230 can be driven by a stepper motor to drive a conveyor belt or a rotary table, which will drive the MxA reagent strip through the photometry and incubation unit 240 and the reading unit 250 in sequence. The conveying unit 230 can be equipped with a sensor to monitor the position of the MxA reagent strip to ensure accurate positioning.

[0024] The photometry and incubation unit 240 is typically conducted in a dark chamber with a controlled temperature, such as 37°C, to provide the necessary environment for the reaction. The incubation time (e.g., 10-15 minutes) is set and adjusted via a timer or conveyor speed. Inside the dark chamber, an integrated fluorescence excitation source (e.g., LED) and a photoelectric sensor scan the detection line (T-line) and control line (C-line) of the card strip to acquire fluorescence intensity data in real time for quantitative analysis.

[0025] In the reading unit 250, the result is determined by the barcode scanner, i.e., the visual recognition system. The result is judged by the instrument algorithm and finally a report is output.

[0026] The entire process from card feeding to result output is automated, supports continuous sample processing, and can close the transmission channel and single-use cards to avoid cross-contamination between samples. In addition, different project cards can be replaced to adapt to the needs of multiple scenarios.

[0027] The sample collection system 300 is used to transport the collected samples to the DIFF reaction detection system 100 or the MxA immunofluorescence detection system 200. It should be understood that when the sample collection system 300 transports the collected samples to the MxA immunofluorescence detection system 200, it mainly transports the samples to the topmost MxA reagent strip. Specifically, the transport can be completed at the MxA reagent strip stacking unit 220 or at the beginning of the transport unit 230.

[0028] The first DIFF reagent line 400 is used to connect to the first DIFF reagent pool 20 and the DIFF reaction detection system 100, so as to guide the first DIFF reagent in the first DIFF reagent pool 20 to the DIFF reaction detection system 100. The second DIFF reagent line 500 is used to connect to the second DIFF reagent pool 30 and the DIFF reaction detection system 100, so as to guide the second DIFF reagent in the second DIFF reagent pool 30 to the DIFF reaction detection system 100.

[0029] The first DIFF reagent pool 20 and the second DIFF reagent pool 30 can be loaded with different reagents (such as hemolysin and staining agents) to support complex testing procedures. Both the first DIFF reagent pool 20 and the second DIFF reagent pool 30 are bottled, so they can automatically select reagents according to the test items, avoiding manual replacement of reagent bottles and improving the continuity of testing.

[0030] In this embodiment, hematology analysis and MxA immunofluorescence detection are integrated on the same platform. The same sample can be used to perform five-part differential hematology analysis and MxA immunofluorescence detection simultaneously, avoiding repeated sampling and dispensing, reducing patient waiting time. The DIFF reaction detection system 100 and the MxA immunofluorescence detection system 200 operate independently, supporting simultaneous detection of routine blood tests and MxA immunofluorescence items, shortening report turnaround time.

[0031] The device provided in this application for identifying viral infections by detecting five-part differential hematology cells and MxA can achieve faster and more accurate identification of viral infections, enabling the detection of viral infections and their severity in an individual with a single and smaller amount of blood sample.

[0032] In some of these embodiments, please refer to Figure 2 The MxA reagent strip stacking unit 220 includes a stand 221, a base plate 222, and a top feeding mechanism 223.

[0033] The stand 221 has a hopper 221a for accommodating MxA reagent strips. The hopper 221a has a discharge port 221b on its upper surface. The hopper 221a serves as a storage container for the MxA reagent strips. The structure of the stand 221 restricts the stacking direction of the strips, ensuring they are arranged vertically. The top discharge port 221b is designed to allow only a single MxA reagent strip to pass through at a time, preventing multiple MxA reagent strips from being ejected simultaneously.

[0034] The base plate 222 is located at the bottom of the upright frame 221. The base plate 222 has a through hole that communicates with the hopper 221a. The base plate 222 supports the stacked MxA reagent strips, and the through hole serves as a channel for the top material component. Its position is usually located in the center of the bottom of the hopper 221a to ensure that the force of the top material component is evenly distributed and to prevent the strips from tilting.

[0035] The top-feeding mechanism 223 is located below the base plate 222. The top-feeding mechanism 223 includes a vertically movable top-feeding component that extends through a through-hole into the storage bin 221a and ejects the uppermost MxA reagent strips out of the storage bin 221a. The top-feeding component is controlled by a motor, cylinder, or electromagnetic drive to reciprocate vertically. When the top-feeding component rises, it lifts the bottommost MxA reagent strips. The lifting height can be controlled by a sensor to ensure that only a single MxA reagent strip is ejected before resetting. The remaining MxA reagent strips fall back to the base plate 222 due to gravity, awaiting the next operation.

[0036] The first pushing unit 210 is used to push the uppermost MxA reagent strip located outside the hopper 221a to the conveying unit 230. After the MxA reagent strip is pushed out of the hopper 221a, it is laterally transferred to the conveying unit 230 by a push rod, a rotary table or a vacuum nozzle. The pushing action is synchronized with the reset of the ejector mechanism 223 to avoid the MxA reagent strip from being stuck in the outlet 221b and causing blockage.

[0037] In this way, the card strips can be supplied "on demand" without manual intervention, supporting continuous sample processing, significantly improving the detection throughput. The mechanical limit and sensor coordinated control ensure that only one card strip is ejected each time, avoiding multiple strips sticking together or being pushed empty, and reducing the system failure rate.

[0038] Furthermore, please continue reading Figure 2 The MxA reagent strip stacking unit 220 includes a mounting plate 224, multiple uprights 221, multiple base plates 222, and a rotating mechanism 225. The multiple uprights 221 are distributed on the mounting plate 224 along the circumference of the mounting plate 224, and the multiple base plates 222 are correspondingly disposed at the bottom of the multiple uprights 221. The rotating mechanism 225 rotates with the mounting plate 224 to drive the mounting plate 224 to rotate around the axis of the mounting plate 224.

[0039] The uprights 221 distributed around the mounting plate 224 form independent storage bins 221a. Each storage bin 221a can store one or more MxA reagent strips. The number of uprights 221 can be increased or decreased according to needs (e.g., 4 / 6 / 8), supporting parallel testing of multiple items.

[0040] It should be understood that the top material mechanism 223 is located below the mounting plate 224, so the mounting plate 224 is provided with a clearance hole that communicates with the through hole of the bottom plate 22, so that the top material can extend into the hopper 221a through the clearance hole and the through hole.

[0041] The mounting plate 224 is rotated around its axis by a stepper motor or servo motor, enabling the switching of the stand 221 positions. An encoder or photoelectric sensor ensures positioning accuracy (e.g., ±0.1°). When the satisfactory chromatography strips in one stand 221 are about to run out, the system automatically rotates to the next stand 221, achieving seamless feeding.

[0042] The multi-stand 221 design supports batch loading, and combined with the rotating mechanism 225, it achieves "rotational feeding," thus enabling non-stop feeding. It supports multiple projects in parallel, such as combined detection of infection markers without stopping the machine to change cards, and the ring layout allows for the miniaturization of the entire device.

[0043] In some embodiments, the device for detecting five-part differential hematology cells and MxA to identify viral infections further includes an HGB detection system 600 and a WBC reagent line 700. The HGB detection system 600 includes a WBC (White Blood Cell) pool, an RBC (Red Blood Cell) pool, and a negative pressure chamber 630. The negative pressure chamber 630 is connected to the WBC pool 610 and the RBC pool 620, respectively. The WBC reagent line 700 is used to connect the WBC reagent pool 40 and the WBC pool 610 to guide the WBC reagent in the WBC reagent pool 40 to the WBC pool 610. The sample collection system 300 is also used to transport the collected samples to the WBC pool 610 and the RBC pool 620.

[0044] The WBC chamber 610 receives the white blood cell portion of the sample, reacts with the WBC reagent (such as red blood cell lysis), and releases the white blood cells for subsequent classification and counting. The chamber may integrate a stirring mechanism or ultrasonic oscillation to ensure that the reagent and sample are thoroughly mixed.

[0045] The RBC 620 directly analyzes red blood cell-related parameters (such as hemoglobin concentration and hematocrit) and can be detected by spectrophotometry or impedance analysis. The independent cell structure avoids interference from leukocyte lysis on red blood cell detection.

[0046] The WBC reagent line 700 precisely delivers the hemolysin (such as quaternary ammonium salt) or staining agent (such as fluorescent dye) in the WBC reagent pool 40 to the WBC pool 610. A three-way valve and an electric injection unit can also be installed on the WBC reagent line 700. The three-way valve and the electric injection unit can also achieve precise aspiration of the WBC reagent pool, thereby ensuring the accuracy of the test.

[0047] The negative pressure chamber 630 generates negative pressure through a vacuum pump, driving the sample and reagents to flow between the chambers. This eliminates the need for a mechanical pump, simplifying fluid control. Cells in the WBC chamber 610 and RBC chamber 620 flow into the negative pressure chamber 630 under the influence of negative pressure. During the flow process, each cell is counted, completing the measurement of WBC and RBC cells (by illuminating the blood sample after the WBC reagent reaction with an LED light, and then calculating the HGB based on the light intensity received by the photodetector).

[0048] In this embodiment, WBC and RBC detection are performed simultaneously, shortening the reporting time. In addition, WBC pool 610 and RBC pool 620 are independent to avoid chemical interference of hemolysing agents on red blood cell detection.

[0049] Furthermore, the device for detecting five-part differential hematology cells and MxA to identify viral infections also includes a waste collection system 800, which is connected to the WBC pool 610, the RBC pool 620, the negative pressure chamber 630, and the DIFF reaction detection system 100.

[0050] Independent pipelines are connected to WBC tank 610, RBC tank 620, negative pressure chamber 630 and DIFF reaction detection system 100 respectively to ensure the separation and treatment of waste liquid from different sources. The liquid level in the tank is monitored by sensors, and the waste liquid is automatically triggered to be discharged to the outside of the entire device. After the reaction is completed in each tank, the sensor sends a signal to the central controller, the valve in the waste liquid collection system 800 is opened, and the negative pressure drives the waste liquid to flow to the collection container in the waste liquid collection system 800.

[0051] It should be understood that after one reaction is completed and before the next reaction is carried out, the WBC tank 610 and RBC tank 620 need to be cleaned to avoid residues that could affect the results of the next reaction. Therefore, in one embodiment of this application, please refer to... Figure 1 The device for detecting five-part differential hematology cells and MxA to identify viral infections also includes a diluent delivery line 900, which is equipped with a three-way valve 930. The three-way valve 930 has one dispensing inlet and two dispensing outlets. The dispensing inlet is used to connect to the diluent pool 10, and the two dispensing outlets are respectively connected to the WBC pool 610 and the RBC pool 620.

[0052] In this embodiment, by using a three-way valve 930, dual-pool liquid supply is achieved through a single valve, reducing the number of pipe joints and lowering the risk of leakage. By reducing the overall length of the pipeline and the internal space occupied by the equipment, a compact layout and miniaturized design can be achieved.

[0053] Following the foregoing, please refer to some embodiments of this application. Figure 1 The diluent delivery pipeline 900 is equipped with a first three-way valve 910 and a first injection unit 920. One port on the first three-way valve 910 is used to connect to the diluent pool 10, and the other two ports on the first three-way valve 910 are respectively connected to the first injection unit 920 and the distribution inlet. The first injection unit 920 is used to introduce the diluent in the diluent pool 10 into the distribution three-way valve.

[0054] The first injection unit 920 is connected to the first interface of the first three-way valve 910, the diluent pool 10 is connected to the second interface of the first three-way valve 910, and the dispensing inlet is connected to the third interface of the first three-way valve 910. The first three-way valve 910 can be a solenoid valve. When drawing diluent, the first interface is connected to the second interface and the first interface is not connected to the third interface. When discharging diluent, the first interface is not connected to the second interface and the first interface is connected to the third interface. Through the first three-way valve 910 and the first injection unit 920, the precise quantitative dispensing of diluent can be achieved to adapt to different detection parameter requirements.

[0055] It should be understood that the first injection unit 920 can be electric, using a motor and ball screw to convert rotary motion into linear motion, thereby facilitating precise control of the piston stroke in the first injection unit 920, and thus precisely controlling the amount of diluent drawn and discharged.

[0056] Optionally, in one embodiment of this application, the sample collection system 300 includes a second three-way valve 310, a second injection unit 320, and a sample collection unit 330. One interface on the second three-way valve 310 is used to communicate with the diluent pool 10, and the other two interfaces on the second three-way valve 310 are respectively connected to the second injection unit 320 and the sample collection unit 330.

[0057] The second injection unit 320 is connected to the first interface of the second three-way valve 310, the diluent pool 10 is connected to the second interface of the second three-way valve 310, and the sample collection unit 330 is connected to the third interface of the second three-way valve 310. The second three-way valve 310 can also be a solenoid valve to realize automatic switching of the connected objects. When the sample is drawn, the first interface and the third interface are connected, and the second interface is closed. When the diluent in the diluent pool 10 is drawn, the first interface and the second interface are connected, and the third interface is closed, so that the diluent and the sample can be mixed. Finally, the sample is discharged through the third interface to the MxA immunofluorescence detection system 200 and the DIFF reaction detection system 100.

[0058] Optionally, the user can also directly draw the sample through the sample collection unit 330 and then discharge it through the third interface. That is, without mixing the sample, the user can draw the diluent from the diluent pool 10 through the second interface and then switch from the second interface to the third interface so that the diluent can be discharged through the sample collection unit 330. This can clean the DIFF reaction detection system 100 and ensure the detection results.

[0059] Thus, through the cooperation of the second injection unit 320 and the second three-way valve 310, the diluent and sample can be accurately aspirated, thereby ensuring the detection results of the MxA immunofluorescence detection system 200 and the DIFF reaction detection system 100.

[0060] It should be understood that the second injection unit 320 can be electric, using a motor and ball screw to convert rotary motion into linear motion, thereby facilitating precise control of the piston stroke in the second injection unit 320 and thus precisely controlling the amount of sample and diluent drawn.

[0061] Optionally, please refer to Figure 1 The first DIFF reagent pipeline 400 is equipped with a third three-way valve 410 and a third injection unit 420. One port on the third three-way valve 410 is used to connect to the first DIFF reagent pool 20, and the other two ports on the third three-way valve 410 are respectively connected to the third injection unit 420 and the DIFF reaction detection system 100. The third injection unit 420 is used to introduce the first DIFF reagent in the first DIFF reagent pool 20 into the DIFF reaction detection system 100.

[0062] The third injection unit 420 is connected to the first interface of the third three-way valve 410, the first DIFF reagent pool 20 is connected to the second interface of the third three-way valve 410, and the DIFF reaction detection system 100 is connected to the third interface of the third three-way valve 410. The third three-way valve 410 can also be a solenoid valve to realize automatic switching of the connected objects. Therefore, the first interface can selectively conduct to the second and third interfaces, so that the first DIFF reagent can be drawn from the first DIFF reagent pool 20 and discharged into the DIFF reaction detection system 100. Through the cooperation of the third injection unit 420 and the third three-way valve 410, the first DIFF reagent can be accurately drawn, thereby ensuring the accuracy of the results.

[0063] Alternatively, please continue reading Figure 1 The second DIFF reagent pipeline 500 is equipped with a fourth three-way valve 510 and a fourth injection unit 520. One port on the fourth three-way valve 510 is used to connect to the second DIFF reagent pool 30, and the other two ports on the fourth three-way valve 510 are respectively connected to the fourth injection unit 520 and the DIFF reaction detection system 100. The fourth injection unit 520 is used to introduce the second DIFF reagent in the second DIFF reagent pool 30 into the DIFF reaction detection system 100.

[0064] The fourth injection unit 520 is connected to the first interface of the fourth three-way valve 510, the second DIFF reagent pool 30 is connected to the second interface of the fourth three-way valve 510, and the DIFF reaction detection system 100 is connected to the third interface of the fourth three-way valve 510. The fourth three-way valve 510 can also be a solenoid valve to realize automatic switching of the connected objects. Therefore, the first interface can selectively conduct to the second and third interfaces, thereby realizing that the second DIFF reagent is drawn from the second DIFF reagent pool 30 and discharged into the DIFF reaction detection system 100. Through the cooperation of the fourth injection unit 520 and the fourth three-way valve 510, the second DIFF reagent can be accurately drawn, thereby ensuring the accuracy of the results.

[0065] It should be understood that the third injection unit 420 and the fourth injection unit 520 can be electric, using a motor and ball screw to convert the rotary motion into linear motion, thereby facilitating precise control of the piston stroke in the third injection unit 420 and the fourth injection unit 520, and thus precisely controlling the amount of first DIFF reagent and second DIFF reagent drawn.

[0066] Specifically, in one embodiment of this application, the DIFF reaction detection system 100 includes a DIFF reaction detection unit 110, a fifth injection unit 120, and a sixth injection unit 130. The DIFF reaction detection unit 110 includes a reaction zone (not shown in the figure) and a detection zone (not shown in the figure). The fifth injection unit 120 is connected to the reaction zone, and the sixth injection unit 130 is connected to the detection zone. The fifth injection unit 120 is used to provide power to push the sample on the reaction zone into the detection zone, and the sixth injection unit 130 is used to provide sheath fluid into the detection zone.

[0067] Under the same conditions, the DIFF reaction detection unit 110 has a laser source, a photodetector, and a flow chamber, and the laser source, photodetector, and flow chamber are all located in the detection area.

[0068] Within the reaction zone, the sample from the sample acquisition system 300 reacts and then, propelled by the fifth injection unit 120, enters the detection zone. The sixth injection unit 130 provides a sheath flow, which encapsulates the sample flow, causing it to queue in the flow chamber and pass through the detection zone. As the sample flow passes through the detection zone encapsulated by the sheath flow, a laser light source illuminates the sample, and a photodetector detects the scattered light or fluorescence signal. The sample, encapsulated in the sheath flow, queues to pass through the flow chamber, completing the measurement of the DIFF channel (white blood cell differential detection).

[0069] The fifth injection unit 120 can be a two-injector system, where one syringe draws in the reacted sample and another syringe discharges the reacted sample. Specifically, the operation of a syringe can be controlled by a solenoid valve, which provides power during discharge so that the sample can move quickly from the reaction zone to the detection zone.

[0070] With the cooperation of the fifth injection unit 120 and the sixth injection unit 130, the sheath flow can wrap the sample flow at a high flow rate and compress the sample flow to the detection spot diameter (5-10μm) through the Bernoulli effect, ensuring that cells pass through the detection area one by one and avoiding overlapping interference.

[0071] The fifth injection unit 120 and the sixth injection unit 130 can also be powered by a piston. The power unit can include a motor and a transmission component. The motor can be a stepper motor or a servo motor, and the transmission component can be a ball screw, so as to precisely control the piston stroke in the fifth injection unit 120 and the sixth injection unit 130.

[0072] In the accompanying drawings of this embodiment, the same or similar reference numerals correspond to the same or similar components. In the description of this application, it should be understood that if terms such as "upper," "lower," "left," and "right" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, they are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the accompanying drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0073] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A device for detecting five-part differential hematology cells and MxA to identify viral infections, for diagnosing an individual's viral infection and its severity, characterized in that, include: DIFF reaction detection system; The MxA immunofluorescence detection system includes a first pushing unit, an MxA reagent strip stacking unit, a conveying unit, a photometric and incubation unit, and a reading unit. The MxA reagent strip stacking unit is used to stack MxA reagent strips. The first pushing unit is used to push the topmost MxA reagent strip of the stacking unit to the conveying unit. The conveying unit is used to transport the MxA reagent strips sequentially through the photometric and incubation unit and the reading unit. A sample collection system is used to transport the collected samples to the DIFF reaction detection system or the MxA immunofluorescence detection system; The first DIFF reagent line is used to connect to the first DIFF reagent pool and the DIFF reaction detection system, so as to guide the first DIFF reagent in the first DIFF reagent pool to the DIFF reaction detection system. The second DIFF reagent line is used to connect to the second DIFF reagent pool and the DIFF reaction detection system, so as to guide the second DIFF reagent in the second DIFF reagent pool to the DIFF reaction detection system.

2. The device for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 1, characterized in that, The MxA reagent strip stacking unit includes: A stand has a hopper for receiving MxA reagent strips, and the hopper has a discharge port on the upper surface of the stand. A base plate is provided at the bottom of the upright frame, and a through hole communicating with the hopper is provided on the base plate; The material feeding mechanism is located below the base plate. The material feeding mechanism includes a vertically movable material feeding component that extends into the hopper through the through hole and pushes the uppermost MxA reagent strip out of the hopper. The first pushing unit is used to push the MxA reagent strip located at the top layer outside the hopper to the conveying unit.

3. The device for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 2, characterized in that, The MxA reagent strip stacking unit includes: Mounting plate; Multiple uprights are distributed on the mounting plate along the circumference of the mounting plate; Multiple base plates, each corresponding to one of the multiple uprights; and A rotating mechanism that rotates with the mounting plate to drive the mounting plate to rotate about its axis.

4. The device for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 1, characterized in that, The device for detecting five-part differential hematology cells and MxA to identify viral infections also includes: The HGB detection system includes a WBC pool, an RBC pool, and a negative pressure chamber, wherein the negative pressure chamber is connected to both the WBC pool and the RBC pool; and WBC reagent tubing is used to connect to the WBC reagent pool and the WBC pool, so as to guide the WBC reagent in the WBC reagent pool to the WBC pool. The sample acquisition system is also used to transport the acquired samples to the WBC pool and the RBC pool.

5. The apparatus for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 4, characterized in that, The device for detecting five-part differential hemoglobin and MxA to identify viral infections also includes a waste collection system, wherein the waste collection unit is connected to the WBC pool, the RBC pool, the negative pressure chamber, and the DIFF reaction detection system.

6. The apparatus for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 4, characterized in that, The device for detecting five-part differential hemoglobin and MxA to identify viral infection also includes a diluent delivery line, which is equipped with a three-way valve. The three-way valve has a dispensing inlet and two dispensing outlets. The dispensing inlet is used to communicate with the diluent pool, and the two dispensing outlets are respectively connected to the WBC pool and the RBC pool.

7. The apparatus for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 6, characterized in that, The diluent delivery pipeline is equipped with a first three-way valve and a first injection unit. One port on the first three-way valve is used to connect to the diluent pool, and the other two ports on the first three-way valve are respectively connected to the first injection unit and the dispensing inlet. The first injection unit is used to introduce the diluent from the diluent pool into the dispensing three-way valve.

8. The apparatus for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 1, characterized in that, The sample collection system includes a second three-way valve, a second injection unit, and a sample collection unit. One interface on the second three-way valve is used to connect to the diluent pool, and the other two interfaces on the second three-way valve are respectively connected to the second injection unit and the sample collection unit.

9. The apparatus for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 1, characterized in that, The first DIFF reagent pipeline is equipped with a third three-way valve and a third injection unit. One port of the third three-way valve is used to connect to the first DIFF reagent pool, and the other two ports of the third three-way valve are respectively connected to the third injection unit and the DIFF reaction detection system. The third injection unit is used to introduce the first DIFF reagent in the first DIFF reagent pool into the DIFF reaction detection system. And / or, the second DIFF reagent pipeline is provided with a fourth three-way valve and a fourth injection unit. One port of the fourth three-way valve is used to connect to the second DIFF reagent pool, and the other two ports of the fourth three-way valve are respectively connected to the fourth injection unit and the DIFF reaction detection system. The fourth injection unit is used to introduce the second DIFF reagent in the second DIFF reagent pool into the DIFF reaction detection system.

10. The apparatus for detecting five-part differential hematology cells and MxA to identify viral infections according to claim 1, characterized in that, The DIFF reaction detection system includes a DIFF reaction detection unit, a fifth injection unit, and a sixth injection unit. The DIFF reaction detection unit includes a reaction zone and a detection zone. The fifth injection unit is connected to the reaction zone, and the sixth injection unit is connected to the detection zone. The fifth injection unit is used to provide power to push the sample on the reaction zone into the detection zone, and the sixth injection unit is used to provide sheath fluid into the detection zone.

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