Multi-collagen composition and eye anti-aging and tightening essence

Abstract

The invention discloses a multi-collagen composition and an eye anti-aging and tightening essence. The multi-collagen composition contains a mussel extract, a leech extract and a salmon skin extract, is prepared by adopting a modern bioengineering technology, and has the multi-channel effects of promoting eye blood circulation, accelerating metabolism, repairing small wound surfaces of skin, accelerating regeneration of collagen and elastin and the like. Tests prove that the eye anti-aging and firming essence containing the multi-collagen composition has a good nursing effect on the eye aging problem and has remarkable anti-aging and firming effects.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, specifically to a multi-collagen composition and an anti-aging and firming serum for the eyes. Background Technology

[0002] The aging process begins around the eyes, mainly due to a variety of factors such as thin skin around the eyes, frequent eye movements, accelerated collagen loss, weak blood circulation, and concentrated ultraviolet radiation damage.

[0003] The skin around the eyes is only 1 / 3 to 1 / 5 the thickness of other parts of the face, with fewer collagen and elastin fibers in the dermis and a thinner subcutaneous fat layer. This structure results in weak support for the skin around the eyes, making it more prone to sagging and drooping due to gravity or external forces. For example, with age, loss of orbital fat can lead to sunken eyes, while decreased skin elasticity can easily cause eye bags or tear troughs.

[0004] The eyes are one of the most expressive areas. Frequent blinking, laughing, and frowning repeatedly stretch the skin, causing collagen fibers to break. When we are young, our skin has good elasticity, and dynamic wrinkles (such as crow's feet) can recover on their own. However, as collagen and hyaluronic acid in the dermis decrease, dynamic wrinkles gradually turn into static wrinkles that are difficult to remove.

[0005] The area around the eyes has a dense network of capillaries, but blood flow is relatively slow. Staying up late and excessive eye strain can lead to poor local blood circulation, causing metabolic waste (such as free radicals and lactic acid) to accumulate, resulting in dark circles and puffiness. Long-term oxygen deficiency can also impair cell repair capabilities and accelerate skin aging.

[0006] The skin around the eyes is sensitive to ultraviolet (UV) radiation. UVA rays can penetrate the dermis, damaging collagen and elastin fibers, leading to sagging skin and darkening of age spots. Furthermore, prolonged exposure to blue light (such as from electronic screens) can exacerbate oxidative damage.

[0007] Therefore, the causes of eye aging are very complex, but researchers can often only grasp one of the causes, resulting in inconsistent effects of the cosmetics they develop for eye aging, with large differences in individual consumer feedback.

[0008] The inventors of this application, through in-depth understanding of the causes of aging skin around the eyes, have developed an eye anti-aging and firming essence containing a multi-collagen composition. This essence promotes blood circulation around the eyes, accelerates metabolism, repairs minor skin wounds, and accelerates the regeneration of collagen and elastin through multiple pathways. Experiments have demonstrated that the eye anti-aging and firming essence prepared according to this invention has a good effect on improving eye aging problems and exhibits significant anti-aging and firming effects, thus completing this invention. Summary of the Invention

[0009] The first objective of this invention is to provide a multi-collagen composition that has multiple pathway effects, such as promoting blood circulation in the eyes, accelerating metabolism, repairing minor skin wounds, and accelerating the regeneration of collagen and elastin.

[0010] A multi-collagen composition containing mussel extract, leech extract and salmon skin extract.

[0011] The mussel extract described in this invention has the cosmetic standard Chinese name "Mussel Extract". Marine mussels, such as the purple mussel, thick-shelled mussel, and jade mussel, use mussel adhesive protein secreted by their byssal threads to anchor themselves to solid surfaces such as rocks, ship hulls, cables, and drifting bottles underwater, forming a water-resistant bond and resisting the erosion of wind and waves. Modern research shows that mussel adhesive protein has the functions of promoting cell adhesion and crawling, promoting wound healing, inhibiting itching, broad-spectrum adhesion, and forming a water-resistant protective film.

[0012] The leech extract described in this invention has the cosmetic standard Chinese name "leech extract". Leeches are a type of animal belonging to the class Annelidae, subclass Hirudinea, phylum Annelida, also known as bloodsuckers. Leeches are an important source of traditional Chinese medicine. After processing into medicinal slices, they have the effects of breaking up blood stasis and promoting menstruation, removing blood stasis and reducing swelling, and can be used for diseases such as amenorrhea due to blood stasis, abdominal masses, hemiplegia due to stroke, and injuries from falls. Leeches contain abundant hirudin, which has a very strong inhibitory effect on thrombin and is the strongest natural specific inhibitor of thrombin discovered to date, exhibiting strong anticoagulant and antithrombotic effects.

[0013] The salmon skin extract described in this invention has the cosmetic standard Chinese name "collagen extract." The salmon used in this invention is Atlantic salmon. Salmon is rich in nutrients, especially vitamins, and is characterized by high protein, low calories, and low cholesterol. Salmon skin is rich in collagen, primarily type I collagen (approximately 80%), which is similar to the collagen type found in human skin and bones, exhibiting good biocompatibility.

[0014] This invention discloses a method for preparing the multiple collagen composition, comprising the following steps:

[0015] (1) Clean the mussels, leeches and salmon skin, add buffer solution, put them into a tissue homogenizer for tissue disruption, then freeze and centrifuge to obtain the supernatant;

[0016] (2) Add sodium chloride to the supernatant, stir slowly, and then freeze and centrifuge to obtain a precipitate; put the precipitate into acetic acid solution, add pepsin to hydrolyze it, and obtain the hydrolysate;

[0017] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through an ultrafiltration membrane to obtain the filtrate; add mannitol, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0018] In some embodiments, the weight ratio of mussels, leeches, and salmon skin in step (1) is 1-10:1-10:1-10.

[0019] In some embodiments, the buffer solution in step (1) is a phosphate buffer solution with a pH of 7.0-8.0, a temperature of 1-5°C, and a material-to-liquid ratio of 1:3-5.

[0020] In some embodiments, the refrigerated centrifugation in step (1) is centrifugation at 1-5°C and 8000-10000 rpm for 15-20 min.

[0021] In some embodiments, the amount of sodium chloride added in step (2) is 5-15% of the weight of the supernatant.

[0022] In some embodiments, the refrigerated centrifugation in step (2) is centrifugation at 1-5°C and 8000-10000 rpm for 15-20 min.

[0023] In some embodiments, the concentration of the acetic acid solution in step (2) is 0.5-1M, and the amount of acetic acid solution used is 10-50 times the weight of the precipitate; the amount of pepsin added is 1000-5000 U / g substrate, and the pepsin is added for 5-10 hours.

[0024] In some embodiments, the ultrafiltration membrane in step (3) has a molecular weight cutoff of 10 kDa.

[0025] In some embodiments, the amount of mannitol added in step (3) is 4-6% of the weight of the filtrate.

[0026] Another objective of this invention is to provide an eye anti-aging and firming serum with significant anti-aging and firming effects.

[0027] An eye anti-aging and firming serum contains the following components in percentage: butylene glycol 3-5%, glycerin 1-5%, PEG / PPG-14 / 7 dimethyl ether 0.5-1%, 1,2-hexanediol 0.5-1%, glyceryl glucoside 0.1-1%, p-hydroxyacetophenone 0.4-0.6%, panthenol 0.1-1%, 1,2-pentanediol 0.5-1%, Tremella fuciformis fruiting body extract 0.01-0.05%, the above-mentioned multiple collagen composition 0.5-5%, and water as the balance.

[0028] The beneficial effects of the technical solution provided in this invention are as follows: The multi-collagen composition provided in this invention, prepared using modern bioengineering technology, has multiple pathways of action, including promoting blood circulation in the eyes, accelerating metabolism, repairing minor skin wounds, and accelerating the regeneration of collagen and elastin. Experiments have shown that the eye anti-aging and firming essence containing the above-mentioned multi-collagen composition has a good care effect on eye aging problems and has significant anti-aging and firming effects. Attached Figure Description

[0029] Figure 1 Test Example 3: Results of VISIA's percentage of crow's feet area;

[0030] Figure 2 Test Example 3: Valid volunteer case.

[0031] In the attached figure, "ns" indicates no statistical difference, "*" indicates 0.01 ≤ p < 0.05, "**" indicates 0.001 ≤ p < 0.01, and "***" indicates p < 0.001. Detailed Implementation

[0032] The embodiments described in this specification are for illustrative purposes only and do not limit the scope of protection of this invention. The scope of protection of this invention is defined only by the claims, and any omissions, substitutions, or modifications made based on the embodiments disclosed in this invention will fall within the scope of protection of this invention.

[0033] To enhance understanding of the present invention, the present invention will be further described in detail below with reference to embodiments. These embodiments are only used to explain the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0034] Example 1

[0035] A multi-collagen composition is prepared by the following steps:

[0036] (1) Clean the mussels, leeches and salmon skin in a weight ratio of 1:10:1, add phosphate buffer solution with pH 8.0 and temperature 1℃, and control the material-liquid ratio to 1:5; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 1℃ and 10000rpm for 15min to obtain the supernatant.

[0037] (2) Add sodium chloride at a weight of 15% of the supernatant, stir slowly, and then freeze and centrifuge at 1°C and 10,000 rpm for 15 min to obtain a precipitate; mix the precipitate with 1M acetic acid solution, the amount of which is 10 times the weight of the precipitate; add pepsin at a ratio of 5000 U / g substrate, and stir slowly for 5 hours to obtain the enzymatic hydrolysate;

[0038] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 6% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0039] Example 2

[0040] A multi-collagen composition is prepared by the following steps:

[0041] (1) Clean the mussels, leeches and salmon skin in a weight ratio of 10:1:10, add phosphate buffer solution with pH 7.0 and temperature 5℃, and control the material-liquid ratio to 1:3; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 5℃ and 8000rpm for 20min to obtain the supernatant.

[0042] (2) Add sodium chloride at a weight of 5% of the supernatant, stir slowly, and then freeze and centrifuge at 5℃ and 8000rpm for 20min to obtain a precipitate; mix the precipitate with 0.5M acetic acid solution, the amount of which is 50 times the weight of the precipitate; add pepsin at a ratio of 1000U / g substrate, and stir slowly for 10 hours to obtain the enzymatic hydrolysate;

[0043] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 4% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0044] Example 3

[0045] A multi-collagen composition is prepared by the following steps:

[0046] (1) Clean the mussels, leeches and salmon skin in a weight ratio of 8:6:3, add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-liquid ratio to 1:4; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0047] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0048] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0049] Comparative Example 1

[0050] A multi-collagen composition is prepared by the following steps:

[0051] (1) Clean the mussels and add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-liquid ratio to 1:4; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0052] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0053] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0054] Comparative Example 2

[0055] A multi-collagen composition is prepared by the following steps:

[0056] (1) Clean the leeches, add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-to-liquid ratio to 1:4; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0057] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0058] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0059] Comparative Example 3

[0060] A multi-collagen composition is prepared by the following steps:

[0061] (1) Clean the salmon skin, add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-to-liquid ratio to 1:4; put it into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0062] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0063] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0064] Comparative Example 4

[0065] A multi-collagen composition is prepared by the following steps:

[0066] (1) Clean the mussels and leeches with a weight ratio of 8:6, add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-liquid ratio to 1:4; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0067] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0068] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0069] Comparative Example 5

[0070] A multi-collagen composition is prepared by the following steps:

[0071] (1) Clean the leeches and salmon skin with a weight ratio of 6:3, add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-liquid ratio to 1:4; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0072] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0073] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0074] Comparative Example 6

[0075] A multi-collagen composition is prepared by the following steps:

[0076] (1) Clean the mussels and salmon skins in a weight ratio of 8:3, add phosphate buffer solution with pH 7.5 and temperature 3℃, and control the material-liquid ratio to 1:4; put them into a tissue homogenizer for tissue disruption, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain the supernatant.

[0077] (2) Add sodium chloride at a weight of 10% of the supernatant, stir slowly, and then freeze and centrifuge at 3℃ and 9000rpm for 20min to obtain a precipitate; mix the precipitate with 0.8M acetic acid solution, the amount of which is 20 times the weight of the precipitate; add pepsin at a ratio of 3000U / g substrate, and stir slowly for 8 hours to obtain the enzymatic hydrolysate;

[0078] (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through a 10kDa ultrafiltration membrane to obtain the filtrate; add 5% mannitol by weight of the filtrate, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

[0079] Test Example 1: Elastase Inhibition Test

[0080] Elastin is a relatively abundant protein in human skin, giving it elasticity and resilience. As we age, the skin's ability to synthesize elastin gradually declines, and the elastin in the skin is continuously broken down by elastase, which is one of the main causes of skin aging.

[0081] Elastase inhibition rate refers to the ability to inhibit elastase in the skin. Studies have shown that by increasing the elastase inhibition rate, the breakdown of elastin in the skin can be reduced, thereby preventing and delaying skin aging.

[0082] The test was conducted in accordance with the method described in the existing technology CN116948961B - A leukocyte extract, an anti-wrinkle and firming face cream and its preparation method.

[0083] Test samples: Multiple collagen compositions prepared in Examples 1-3 and Comparative Examples 1-6.

[0084] All experimental samples were diluted to a 5% aqueous solution in 96-well plates, and then elastic receptor solution and elastase were added sequentially. After thorough mixing, the plates were allowed to stand at room temperature for 20 minutes, and the absorbance was measured at 405 nm. Each measurement was performed in triplicate.

[0085] Elastase inhibition rate (%) = (1-CD / AB) × 100;

[0086] A: Elastase present, no sample found; B: Elastase absent, sample present; C: Elastase present, no sample found; D: Elastase absent, sample present.

[0087] The specific test results are shown in Table 1:

[0088] Table 1 shows the elastase inhibition rate of the test samples.

[0089] sample elastase inhibition rate Example 1 85.41％ Example 2 91.20％ Example 3 87.76％ Comparative Example 1 15.35％ Comparative Example 2 27.89％ Comparative Example 3 17.92％ Comparative Example 4 45.66％ Comparative Example 5 44.13％ Comparative Example 6 35.47％

[0090] As shown in Table 1, the 5% aqueous solution of the multi-collagen composition extracted by the method of the present invention has an elastase inhibition rate of over 85%, which significantly inhibits elastase activity, prevents the decomposition of elastin fibers, and has a good anti-wrinkle and firming effect.

[0091] Test Example 2: Safety Assessment Test

[0092] Human safety testing was conducted according to the "Cosmetic Safety Technical Specifications" (2015 edition), specifically using the occlusive patch test method. Qualified patch testing equipment with an area not exceeding 50 mm² and a depth of approximately 1 mm was selected. The test substance was placed in the patch testing chamber at a volume of approximately 0.020 mL–0.025 mL (liquid). The test substance was the multiple collagen composition prepared in Preferred Example 3, and the control well served as a blank control (without any substance). The patch testing device containing the test substance was applied to the back or flexor side of the forearm of the subject using hypoallergenic adhesive tape, and the palm was gently pressed to ensure even application to the skin for 24 hours. Skin reactions were observed according to the standards in Table 1 at 30 minutes (after the indentation disappeared), 24 hours, and 48 hours after removal of the patch testing device. The observation results were recorded.

[0093] Table 2 Summary of Skin Patch Test Results

[0094]

[0095] Table 2 shows the results of the human skin patch test, with 0 cases of adverse skin reactions observed in 32 participants. This demonstrates that the multi-collagen composition prepared in this embodiment of the invention has good safety.

[0096] Example 4

[0097] An eye anti-aging and firming serum contains the following components in percentage: 3% butylene glycol, 5% glycerin, 0.5% PEG / PPG-14 / 7 dimethyl ether, 1% 1,2-hexanediol, 0.1% glyceryl glucoside, 0.6% p-hydroxyacetophenone, 0.1% panthenol, 1% 1,2-pentanediol, 0.01% Tremella fuciformis fruiting body extract, 0.5% the multi-collagen composition prepared in Example 1, and the balance being water.

[0098] Example 5

[0099] An eye anti-aging and firming serum contains the following components in percentage: butylene glycol 5%, glycerin 1%, PEG / PPG-14 / 7 dimethyl ether 1%, 1,2-hexanediol 0.5%, glyceryl glucoside 1%, p-hydroxyacetophenone 0.4%, panthenol 1%, 1,2-pentanediol 0.5%, Tremella fuciformis fruiting body extract 0.05%, the multi-collagen composition prepared in Example 3 5%, and water as the balance.

[0100] Test Example 3

[0101] The human efficacy evaluation of the eye anti-aging and firming essence prepared in Example 5 was carried out in accordance with the "T / CAB 0152-2022 Test Methods for Seven Efficacy Items of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing and Soothing".

[0102] Volunteer selection: 32 people had fine lines or wrinkles at the corners of their eyes, which met the level 1-6 of the "Skin Aging Atlas" classification chart, and the wrinkles at the left and right outer corners of their eyes were of the same level.

[0103] Test Procedure: Before the test, the test area was cleansed using a standardized facial cleanser. The percentage of wrinkles around the eyes was collected using VISA-CR on enrolled subjects and recorded as the initial value. During the photo session, subjects closed their eyes and remained relaxed. Test samples were distributed; after cleansing, volunteers applied an appropriate amount of sample around the corners of their eyes, gently massaging in circular motions until absorbed, twice daily (morning and evening). At the designated test time (28 days after product use), the percentage of wrinkles around the eyes was collected again using PRIMOS-CR and recorded as the measured value. Detailed test results are attached. Figure 1 .

[0104] From the appendix Figure 1-2The results showed that after 14 days of continuous use of the experimental eye anti-aging and firming serum, the proportion of VISIA crow's feet wrinkles did not change significantly compared to the baseline value. However, after 28 days of continuous use, the proportion of VISIA crow's feet wrinkles significantly decreased compared to the baseline value (p < 0.05), with a decrease rate of 6.68%. This demonstrates that the eye anti-aging and firming serum achieved good anti-aging and firming effects.

[0105] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0106] The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A multi-collagen composition, characterized in that, It contains mussel extract, leech extract and salmon skin extract.

2. The multiple collagen composition according to claim 1, characterized in that, The preparation method of the multiple collagen composition includes the following steps: (1) Clean the mussels, leeches and salmon skin, add buffer solution, put them into a tissue homogenizer for tissue disruption, then freeze and centrifuge to obtain the supernatant; (2) Add sodium chloride to the supernatant, stir slowly, and then freeze and centrifuge to obtain a precipitate; put the precipitate into acetic acid solution, add pepsin to hydrolyze it, and obtain the hydrolysate; (3) Adjust the pH of the enzymatic hydrolysate to neutral, pass it through an ultrafiltration membrane to obtain the filtrate; add mannitol, stir to dissolve, and then transfer it to a vacuum freeze dryer to freeze dry, thus obtaining the multi-layer collagen composition.

3. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The weight ratio of mussels, leeches, and salmon skin mentioned in step (1) is 1-10:1-10:1-10.

4. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The buffer solution mentioned in step (1) is a phosphate buffer solution with a pH of 7.0-8.0, a temperature of 1-5℃, and a material-to-liquid ratio of 1:3-5.

5. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The refrigerated centrifugation in step (1) is centrifugation at 1-5℃ and 8000-10000rpm for 15-20min.

6. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The amount of sodium chloride added in step (2) is 5-15% of the weight of the supernatant.

7. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The refrigerated centrifugation in step (2) is centrifugation at 1-5℃ and 8000-10000rpm for 15-20min.

8. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The concentration of the acetic acid solution in step (2) is 0.5-1M, and the amount of acetic acid solution used is 10-50 times the weight of the precipitate; the amount of pepsin added is 1000-5000 U / g substrate, and the pepsin is added for 5-10 hours.

9. The method for preparing the multiple collagen composition according to claim 2, characterized in that, The ultrafiltration membrane in step (3) has a molecular weight cutoff of 10 kDa; the amount of mannitol added is 4-6% of the weight of the filtrate.

10. An eye anti-aging and firming serum, characterized in that, It contains the following components in percentage: 3-5% butylene glycol, 1-5% glycerin, 0.5-1% PEG / PPG-14 / 7 dimethyl ether, 0.5-1% 1,2-hexanediol, 0.1-1% glyceryl glucoside, 0.4-0.6% p-hydroxyacetophenone, 0.1-1% panthenol, 0.5-1% 1,2-pentanediol, 0.01-0.05% Tremella fuciformis fruiting body extract, 0.5-5% of the multiple collagen composition according to any one of claims 1-9, and water as the balance.

Images