Ocean sipunculid capsule preparation device and preparation method thereof
The marine sipunculus capsule preparation device, which integrates a vortex generation system, a micro-mixing system, and a flocculation growth system, solves the problems of uniformity and stability in the precipitation process of marine sipunculus extract in traditional stirred tanks, and achieves the preparation of high-purity, high-bioactivity capsules.
Patent Information
- Application Number
- CN202511621493.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-07
- Publication Date
- 2026-01-23
AI Technical Summary
In the industrial production of marine sipunculus extract, existing technologies often fail to achieve uniform dispersion of precipitants in traditional stirred tanks, leading to reduced product purity and decreased bioactivity. Furthermore, the high dependence on operational experience results in unstable product quality.
The system employs a combination of a vortex generation system, a micro-mixing system, and a flocculation growth system with a zoned temperature control structure. It achieves uniform mixing of the precipitant through stable rotating vortices and micro-atomized spraying, and provides a low-shear, long-retention environment in the flocculation growth system to allow the precipitated particles to grow fully. Combined with a precision fluid dynamics design and a temperature control system, the controllability of process parameters is ensured.
It significantly improved the purity and bioactivity of marine sipunculus extract, increased process yield, ensured product quality stability and consistency, and reduced reliance on operational experience.
Smart Images
Figure CN121372297A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biological product processing equipment, and particularly relates to a marine sipunculan capsule preparation device and a preparation method thereof. BACKGROUND
[0002] The marine sipunculan is a marine organism with both nutritional and medicinal values, and is rich in protein, amino acids and various trace elements, and is a high-grade nutritional health food. The marine sipunculan is often used as a food therapy medicine for nourishing yin and kidney and treating hyperactivity of deficiency fire in folk, and is called as "marine Cordyceps sinensis" and "animal ginseng" due to its significant anti-fatigue and anti-aging effects.
[0003] In order to standardize the extraction and development of active ingredients in the marine sipunculan, the preparation method of the marine sipunculan extract in the prior art usually includes obtaining an extraction supernatant by salt solution extraction, and then performing fractional precipitation by using an organic solvent to separate and purify the extract containing active proteins (including sipunculan kinase), taurine and other effective components. Specifically, a core step of the method is to treat the extraction supernatant with anhydrous ethanol so that the final concentration reaches 20%-70%, so that the target active component is precipitated and separated out.
[0004] However, when the above method is directly scaled up to an industrial production scale, if a conventional general stirred tank is used as a precipitation device, a series of technical bottlenecks will be encountered. Poor mixing uniformity leads to reduced product purity. The marine sipunculan extraction supernatant usually has high viscosity, and ethanol as a precipitant needs to be quickly and uniformly dispersed. The conventional mechanical stirring paddle is difficult to achieve instantaneous and molecular-level uniform mixing, and it is easy to produce a local area with excessively high ethanol concentration near the feeding point or the paddle blade. Such non-uniformity can cause partial target active proteins to be denatured and inactivated due to encountering excessively high concentration of organic solvent, or cause impure proteins to be wrapped and co-precipitated, thereby affecting the purity and biological activity of the final product.
[0005] In the turbulent and irregular turbulent environment of the conventional stirred tank, the precipitated protein precipitate particles are usually small in size, loose in structure and low in density. Such poor physical property of the precipitate makes it difficult to be efficiently captured in the subsequent solid-liquid separation processes such as centrifugation and filtration, and a large amount of target product is easily lost with the mother liquor, which significantly reduces the overall yield of the process.
[0006] The effect of traditional stirring and precipitation is highly dependent on the cooperation of multiple parameters such as feeding speed, stirring speed, temperature, and the like, and the optimal process window is very narrow. In actual production, the experience of the operator has a great influence on the product quality, and there are large fluctuations in purity, activity and particle morphology between different batches of products. In order to realize more stable and standardized large-scale production, therefore, there is an urgent need in the art to develop a new type of preparation device and a matching method to solve the above problems in the industrialized precipitation process of marine starfish extract. SUMMARY
[0007] The purpose of the present application is to solve the defects in the prior art and realize technical upgrading.
[0008] In order to achieve the above purpose, the present application adopts the following technical scheme: A marine starfish capsule preparation device comprises: A vertical tank body; A vortex generating system arranged at the upper part of the vertical tank body for forming a rotating vortex in the material entering the vertical tank body; A micro-mixing system arranged in the vertical tank body and located on the movement path of the rotating vortex for adding a precipitant to the rotating vortex; A flocculation growth system arranged inside the vertical tank body, the feed inlet of the flocculation growth system being in fluid communication with the lower part of the vertical tank body, and the discharge outlet being located at the upper part thereof.
[0009] Further, the vortex generating system comprises a involute spiral feed flow channel which is arranged outside the upper part of the vertical tank body and is in tangential communication therewith.
[0010] Further, at least two spiral guide ribs extending downward along the rotation direction of the vortex are arranged on the inner wall of the upper part of the vertical tank body. The pitch of the spiral guide ribs gradually decreases from top to bottom.
[0011] Further, the micro-mixing system comprises at least two concentrically arranged annular pipes, a plurality of atomizing nozzles are uniformly distributed on each annular pipe, and the jet direction of the atomizing nozzles is adapted to the movement direction of the vortex.
[0012] Further, the atomizing nozzle is a coaxial airflow assisted nozzle comprising a central pipe for conveying a precipitant and an annular outer pipe for conveying high pressure gas.
[0013] Further, the flocculation growth system comprises a central draft tube vertically arranged at a central position of the vertical tank body, and the central draft tube is a variable-diameter structure with a larger lower port cross-sectional area than an upper port cross-sectional area.
[0014] Further, the central draft tube is internally filled with function fillers arranged in layers.
[0015] Further, the function fillers comprise: a coarse flocculation filler layer with a first porosity arranged at a lower portion of the central draft tube; a cultivation filler layer with a second porosity arranged at an upper portion of the central draft tube; The first porosity is greater than the second porosity.
[0016] Further, the vertical tank body further comprises a partitioned temperature control structure, and the partitioned temperature control structure comprises: a first temperature control jacket arranged on an outer wall of the vertical tank body; and a second temperature control flow path embedded in a wall of the central draft tube and independent of the first temperature control jacket.
[0017] A preparation method of a sea starworm capsule, comprising the following steps: After homogenate extraction of sea starworm raw materials by using a phosphate buffer solution containing 1-2 mol / L hydrochloric acid salt, the supernatant material obtained is sent into the vertical tank body through the vortex generation system to form a stable rotating vortex; The micro-mixing system is started, anhydrous ethanol as a precipitant is atomized by the atomizing nozzle and sprayed into the rotating vortex, and the final concentration of ethanol in the vertical tank body is controlled to be 20%-70%, to form a mixed liquid containing preliminary precipitates; The mixed liquid is introduced from a lower portion of the vertical tank body into the central draft tube of the flocculation growth system, and flows from bottom to top in the central draft tube; The mixed liquid sequentially flows through the coarse flocculation filler layer and the cultivation filler layer to obtain a final material liquid with increased precipitated particles, and is discharged from the discharge port to obtain a sea starworm extract; The obtained sea starworm extract is subjected to drying treatment, and the dried powder is subjected to capsule filling to obtain the sea starworm capsule.
[0018] Compared with the prior art, the present application has the following beneficial effects: By combining stable macroscopic eddies with microscopic atomized spraying, instantaneous and uniform mixing of the precipitant and materials is achieved, preventing protein denaturation or co-precipitation of impurities caused by excessively high local concentrations. This significantly improves the purity and bioactivity of the final extract. Simultaneously, the flocculation growth system provides a low-shear, long-retention environment for the precipitated particles. Through variable-diameter deceleration and orderly guidance by graded packing, tiny nascent precipitated particles can grow sufficiently and become dense, significantly improving the physical properties of the precipitate and making it easier to collect in subsequent solid-liquid separation processes, thereby greatly increasing the overall process yield. Furthermore, this invention replaces traditional random mechanical stirring with a precisely designed, controllable fluid dynamics structure, making the entire precipitation and growth process highly ordered and predictable. Combined with a zoned temperature control system for precise regulation of different stages, process parameters are strictly fixed, eliminating reliance on operational experience and ultimately ensuring high consistency and stability of product quality across different production batches. Attached Figure Description
[0019] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof.
[0020] Figure 1 This is a schematic diagram of the overall structure of the marine sipunculus capsule preparation device proposed in this invention; Figure 2 This is a top view of the marine sipunculus capsule preparation device proposed in this invention; Figure 3 This is an internal schematic diagram of the marine sipunculus capsule preparation device proposed in this invention; Figure 4 This is a cross-sectional view of the marine sipuncula capsule preparation apparatus proposed in this invention; Figure 5 This is a schematic diagram illustrating the preparation principle of the marine sipunculus capsule preparation device proposed in this invention; Figure 6 This is a flowchart of the method for preparing marine sipuncula capsules proposed in this invention.
[0021] In the diagram: 1. Vertical tank; 2. Vortex generation system; 21. Feed channel; 22. Spiral guide ribs; 3. Micro-mixing system; 31. Annular pipe; 32. Atomizing nozzle; 33. Central pipe; 34. Annular outer pipe; 4. Flocculation growth system; 41. Central guide tube; 42. Functional packing; 43. Coarse flocculation packing layer; 44. Cultivation packing layer; 5. Zoned temperature control structure; 51. First temperature control jacket; 52. Second temperature control flow path. Detailed Implementation
[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.
[0023] In the description of this invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.
[0024] like Figures 1-5 As shown in the figure, the marine star worm capsule preparation device provided in this embodiment has its structure integrated in a single vertical tank 1. The bottom of the vertical tank 1 is preferably designed to be conical to facilitate the collection and discharge of a small amount of accidentally settled heavy particles or impurities.
[0025] In one specific embodiment, a marine sipuncula capsule preparation device includes a vortex generating system 2, a micro-mixing system 3, a flocculation growth system 4, and a zoned temperature control structure 5, wherein: The vortex generating system 2 includes a volute-type feed channel 21 disposed outside the tank body and a spiral guide rib 22 disposed inside the tank body. The volute-type feed channel 21 is an open spiral channel with a specific cross-section, such as rectangular or circular, that surrounds the upper part of the tank body. The spiral path of the volute-type feed channel 21 follows an involute curve, and its spiral radius gradually decreases from the outside to the inside. The end point of the volute-type feed channel 21 is smoothly tangentially connected to the upper inner wall of the vertical tank body 1 to form a feed inlet. When the supernatant extracted from marine sipuncula, used as the starting material, is pumped into the volute-type feed channel 21, it is smoothly and stably guided, forming a regular rotating vortex with minimal energy loss and shear force upon entering the tank. To further ensure the stability of this vortex during its downward movement, several spiral guide ribs 22 are fixed to the inner wall of the upper part of the vertical tank 1. The spiral direction of the guide ribs 22 is consistent with the rotation direction of the vortex. Their function is to provide a stable guiding trajectory for the rotating fluid, prevent turbulence, and ensure that the vortex rotates downward at a controllable angular and axial velocity.
[0026] In this embodiment, the micro-mixing system 3 consists of a set of fixed, suspended spray assemblies mounted on the upper part of the tank and below the vortex path. The dual-ring spray assembly includes two concentric annular pipes 31 on the same horizontal plane, specifically an outer ring pipe and an inner ring pipe. Multiple coaxial airflow-assisted atomizing nozzles 32 are uniformly installed on both the outer and inner ring pipes in the direction of vortex movement. Each atomizing nozzle 32 is connected to an independent solenoid valve and has a central pipeline for conveying the liquid precipitant anhydrous ethanol and an annular outer pipeline for conveying high-pressure inert gas such as nitrogen. During operation, the solenoid valves on the inner and outer ring pipes are controlled by a PLC program in a time-sequential pulse manner. The high-pressure gas atomizes the anhydrous ethanol into micron-sized droplets and precisely sprays them into the high-speed vortex liquid flowing below.
[0027] The main body of the flocculation growth system 4 is a central guide tube 41 vertically fixed at the center of the vertical tank 1. The central guide tube 41 is designed with a variable diameter shape, wider at the bottom and narrower at the top, meaning that the diameter of its lower inlet port is larger than the diameter of its upper outlet port. This allows the incoming fluid to be significantly decelerated, providing gentle kinetic conditions for particle aggregation. Inside the central guide tube 41, two different types of functional fillers 42 are filled in layers. The lower layer is a coarse flocculation filler layer 43, which uses a structured filler with a large porosity and a small specific surface area. Its main function is to increase the effective collision frequency of particles, causing small particles to aggregate into larger flocs. The upper layer is a cultivation filler layer 44, located above the coarse flocculation filler layer 43. It uses a structured filler with a small porosity and a large specific surface area. Its main function is to provide a large number of attachment growth points for the flocs, allowing them to be further cultivated to become larger and denser during the slow upward movement, forming an ideal crystal form that is easy to separate.
[0028] The zoned temperature control structure 5 is used for precise thermodynamic control of the process. The zoned temperature control structure 5 includes a first temperature control jacket 51 set on the outer wall of the vertical tank 1 for temperature control of the material inside the entire tank. At the same time, an independent second temperature control flow path 52 is also embedded inside the wall of the central guide tube 41. The second temperature control flow path 52 has an independent temperature control medium circulation system. By differentiating the structures of these two temperature control units, zoned temperature control can be achieved. For example, a temperature can be maintained in the external rapid sedimentation zone, while a lower or more constant temperature can be set in the internal slow cultivation zone, thereby optimizing each stage of the entire process to the best thermodynamic conditions.
[0029] like Figure 6 As shown, in an example scenario, the complete method for preparing marine sipuncula capsules using the above-described apparatus is as follows: First, the starting material was prepared by homogenizing the marine sipuncula raw material with a phosphate buffer solution containing 1-2 mol / L hydrochloride and centrifuging to obtain the supernatant.
[0030] Subsequently, the supernatant is pumped into the volute-type feed channel 21 of the device. The material is guided and shaped in the channel and enters the vertical tank 1 from the tangential opening. With the assistance of the spiral guide ribs 22, a stable, downward rotating vortex is formed.
[0031] Next, the micro-mixing system 3 and the zoned temperature control structure 5 are started. The PLC controller starts the atomizing nozzles 32 of the outer and inner rings in sequence according to the preset program, spraying anhydrous ethanol into the vortex in the form of micro-mist until the final concentration of ethanol in the mixture in the tank reaches the target range of 20%-70%. At this time, the active protein is uniformly precipitated and forms a preliminary precipitate.
[0032] The mixture carrying the initial precipitate spirals down along the tank wall and enters the bottom of the central guide tube 41 of the flocculation growth system 4 from the bottom of the tank. Inside the guide tube, the flow rate of the mixture decreases and it passes sequentially from bottom to top through the coarse flocculation packing layer 43 and the cultivation packing layer 44, during which the precipitated particles are fully cultivated and grown.
[0033] Finally, the liquid carrying the mature precipitated particles overflows from the outlet at the top of the central guide tube 41, is collected, and sent to the subsequent solid-liquid separation unit. The separated solid material is a high-purity, high-physical-property marine sipunculus extract.
[0034] Finally, the obtained extract is freeze-dried, and the dried powder is then precisely filled into capsules using a capsule filling machine to obtain the final marine sipunculus capsule product.
[0035] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.
Claims
1. A marine sipuncula capsule preparation apparatus, characterized in that, include: Vertical tank (1); A vortex generating system (2) is provided at the upper part of the vertical tank (1) to generate a rotating vortex in the material entering the vertical tank (1); A micro-mixing system (3) is installed inside the vertical tank (1) and located on the motion path of the rotating vortex, for adding a precipitant to the rotating vortex; The flocculation growth system (4) is located inside the vertical tank (1). The inlet of the flocculation growth system (4) is in fluid communication with the lower part of the vertical tank (1), and its outlet is located at the upper part of itself.
2. The marine sipuncula capsule preparation apparatus according to claim 1, characterized in that, The vortex generating system (2) includes an involute spiral feed channel (21) that surrounds the upper part of the vertical tank (1) and is tangentially connected to it.
3. The marine sipuncula capsule preparation apparatus according to claim 2, characterized in that, On the inner wall of the upper part of the vertical tank (1), at least two spiral guide ribs (22) extending downward along the vortex rotation direction are also provided. The pitch of the spiral guide rib (22) gradually decreases from top to bottom.
4. The marine sipuncula capsule preparation apparatus according to claim 1, characterized in that, The micro-mixing system (3) includes at least two concentric annular pipes (31), each annular pipe (31) is provided with a plurality of atomizing nozzles (32), and the spraying direction of the atomizing nozzles (32) is adapted to the motion direction of the vortex.
5. The marine sipuncula capsule preparation apparatus according to claim 4, characterized in that, The atomizing nozzle (32) is a coaxial airflow-assisted nozzle, including a central pipe (33) for conveying the precipitant and an annular outer pipe (34) for conveying high-pressure gas.
6. The marine sipuncula capsule preparation apparatus according to claim 1, characterized in that, The flocculation growth system (4) includes a central guide tube (41) vertically positioned at the center of the vertical tank (1), and the central guide tube (41) is a variable diameter structure with a lower port cross-sectional area larger than the upper port cross-sectional area.
7. The marine sipuncula capsule preparation apparatus according to claim 6, characterized in that, The interior of the central guide tube (41) is filled with layered functional filler (42).
8. The marine sipuncula capsule preparation apparatus according to claim 7, characterized in that, The functional filler (42) includes: A coarse flocculation packing layer (43) with a first porosity is disposed at the lower part of the central guide tube (41). A cultivation packing layer (44) with a second porosity is disposed on the upper part of the central guide tube (41). The first porosity is greater than the second porosity.
9. The marine sipuncula capsule preparation apparatus according to claim 6, characterized in that, It also includes a zoned temperature control structure (5), which includes: The first temperature control jacket (51) is installed on the outer wall of the vertical tank (1). And a second temperature control flow path (52) embedded in the wall of the central guide tube (41) and independent of the first temperature control jacket (51).
10. A method for preparing a marine sipunculus capsule, characterized in that, This method utilizes the marine sipuncula capsule preparation apparatus according to any one of claims 1-9 to prepare the active ingredient as the capsule contents, and includes the following steps: The supernatant obtained by homogenizing and extracting marine sipunculid raw material with a phosphate buffer solution containing 1-2 mol / L hydrochloride is fed into the vertical tank (1) through the vortex generating system (2) to form a stable rotating vortex. Start the micro-mixing system (3), atomize the anhydrous ethanol as a precipitant through the atomizing nozzle (32) and spray it into the rotating vortex, and control the final concentration of ethanol in the vertical tank (1) to be 20%-70% to form a mixture containing preliminary precipitates; The mixture is introduced from the bottom of the vertical tank (1) into the central guide tube (41) of the flocculation growth system (4), so that it flows from bottom to top in the central guide tube (41); The mixture is passed sequentially through the coarse flocculation packing layer (43) and the cultivation packing layer (44) to obtain a final liquid with enlarged precipitate particles, and discharged from the outlet to obtain marine sipunculus extract; The obtained marine sipunculus extract was dried, and the dried powder was then encapsulated to obtain the marine sipunculus capsules.