Centrifugal nucleic acid microfluidic device

Abstract

The invention discloses a centrifugal nucleic acid micro-fluidic device, and relates to the field of detection equipment, the centrifugal nucleic acid micro-fluidic device comprises a detection box, a movable plate is movably arranged in the detection box, a rotating seat is rotatably arranged at the top of the movable plate, a movable column is movably arranged in the rotating seat, one end of the movable column is connected with a movable block, and the other end of the movable block is connected with a rotating shaft; a first spring is arranged at the top of the movable block, and the top end of the first spring is connected with a connecting block. A micro-fluidic chip is directly placed at the top of a rotating seat, then a movable plate enters a detection box, an electric push rod is started, a movable column moves downwards, a movable block is pushed to move downwards, the movable block pulls a connecting block through a first spring to move downwards along with the connecting block, a clamping block descends under the action of a connecting strip, and the micro-fluidic chip is clamped; according to the structure, the automatic centering operation after the micro-fluidic chip is placed is realized, and the first spring can be elastically adapted after the size of the chip is changed, so that the chip is prevented from being damaged by clamping force.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of detection equipment, in particular to a centrifugal nucleic acid microfluidic device. BACKGROUND

[0002] Nucleic acid detection is a key technology widely used in modern molecular diagnosis, especially in the fields of infectious diseases, tumor marker detection and genetic disease screening, etc. Its core steps include sample lysis, nucleic acid extraction, amplification reaction and detection. The above steps can be completed by microfluidic technology, which has the ability to integrate all functions such as sample pretreatment, reaction and detection on a chip of centimeter size.

[0003] The existing microfluidic chip needs to be placed in the device for centrifugation after the sample is added. However, in the actual working process, the chip position needs to be accurately aligned to perform subsequent centrifugation, otherwise the centrifugation effect of the sample inside the chip will be poor. SUMMARY

[0004] Therefore, the purpose of the present application is to provide a centrifugal nucleic acid microfluidic device to solve the technical problem that the general microfluidic chip needs to be accurately aligned to prevent subsequent centrifugation.

[0005] To achieve the above-mentioned purpose, the present application provides the following technical scheme: a centrifugal nucleic acid microfluidic device, comprising a detection box, an activity plate is movably arranged in the inside of the detection box, a rotating seat is rotatably arranged on the top of the activity plate, an activity column is movably arranged in the inside of the rotating seat, an activity block is connected to one end of the activity column, a first spring is arranged on the top of the activity block, a connecting block is connected to the top end of the first spring, a connecting strip is rotatably connected to the side wall of the connecting block, a clamping block is rotatably connected to one end of the connecting strip, an electric push rod is installed on the top of the detection box, a connecting seat is connected to the output end of the electric push rod, a rotating cylinder is arranged on the bottom of the connecting seat, a rotating mechanism is installed on the top of the connecting seat, and the rotating mechanism controls the rotation of the rotating cylinder.

[0006] Further, the top end of the activity column is connected to a pressing plate, and a through slot matched with the clamping block is formed in the top of the pressing plate.

[0007] Further, an insertion slot is formed in the top of the pressing plate, and an insertion block matched with the insertion slot is arranged on the bottom of the rotating cylinder.

[0008] Further, the rotating mechanism comprises a motor and a gear, the motor is installed on the top of the connecting seat, the output end of the motor is connected with the gear, and a gear ring matched with the gear is installed on the inner wall of the rotating cylinder.

[0009] The detection box is internally provided with a chain transmission mechanism, and a control movable plate of the chain transmission mechanism slides in and out of the detection box.

[0010] The side wall of the electric push rod is provided with a connecting column, the bottom end of the connecting column penetrates through the connecting seat and is connected with a pressing block.

[0011] The inner top wall of the connecting seat is provided with a second spring, the bottom end of the second spring is connected with a guide column, one end of the guide column is connected with a connecting shell, the connecting shell is located on the top of the pressing block, and the connecting column and the top of the pressing block are connected through an electronic buckle.

[0012] The top of the connecting seat is respectively provided with a sewage tank and a clean water tank, the sewage tank and the clean water tank are respectively connected with a clean water pipe and a sewage pipe, one end of the clean water pipe and the sewage pipe respectively penetrates through the connecting shell and the pressing block and extends to the bottom of the pressing block, and a water pump is arranged in the middle of the sewage pipe.

[0013] The bottom of the pressing block is provided with a camera.

[0014] The microfluidic chip is directly placed on the top of the rotating seat, then the movable plate enters the inside of the detection box, the electric push rod is started, the movable column moves downward, the movable block moves downward, the movable block pulls the connecting block downward through the first spring, and the clamping block clamps the microfluidic chip under the action of the connecting strip, the above structure realizes the automatic centering operation after the microfluidic chip is placed, and the first spring can be elastically adapted after the size of the chip changes, so that damage of the clamping force to the chip is prevented. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a first perspective three-dimensional structure schematic view of the application; Figure 2 It is a second perspective three-dimensional structure schematic view of the application; Figure 3 It is an internal structure sectional view of the rotating cylinder of the application; Figure 4 It is a bottom structure schematic view of the pressing block of the application; Figure 5 It is an internal structure sectional view of the rotating plate of the application; Figure 6 It is an internal structure sectional view of the detection box of the application.

[0016] In the diagram: 1. Detection box; 2. Movable plate; 3. Rotating seat; 4. Pressure plate; 5. Movable column; 6. First spring; 7. Movable block; 8. Connecting block; 9. Connecting bar; 10. Clamping block; 11. Slot; 12. Electric push rod; 13. Connecting seat; 14. Rotating cylinder; 15. Connecting shell; 16. Pressure block; 17. Connecting column; 18. Second spring; 19. Guide column; 20. Clean water pipe; 21. Sewage pipe; 22. Water pump; 23. Motor; 24. Gear; 25. Sewage tank; 26. Clean water tank; 27. Camera; 28. Insertion block; 29. ​​Chain drive mechanism. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0018] The embodiments of the present invention will now be described.

[0019] A centrifugal nucleic acid microfluidic device, such as Figures 1-6 As shown, the device includes a detection chamber 1, an movable plate 2 is movably arranged inside the detection chamber 1, and a chain drive mechanism 29 is installed inside the detection chamber 1. The chain drive mechanism 29 controls the movable plate 2 to slide inside and outside the detection chamber 1. When a sample is added to the microfluidic chip, the chip is placed on the pressure plate 4, and then the chain drive mechanism 29 is activated to transport the movable plate 2 into the detection chamber 1.

[0020] Furthermore, the movable plate 2 is composed of two sections of plate, so chip fixing structures can be placed on the surface of both sections of plate, which can effectively improve the detection efficiency.

[0021] The top of the detection box 1 is provided with an electric push rod 12, the output end of the electric push rod 12 is connected with a connecting seat 13, the bottom of the connecting seat 13 is provided with a rotating cylinder 14, the top of the movable plate 2 is rotationally provided with a rotating seat 3, the inside of the rotating seat 3 is movably provided with a movable column 5, one end of the movable column 5 is connected with a movable block 7, the top of the movable block 7 is provided with a first spring 6, the top end of the first spring 6 is connected with a connecting block 8, the side wall of the connecting block 8 is rotationally connected with a connecting strip 9, one end of the connecting strip 9 is rotationally connected with a clamping block 10, the top end of the movable column 5 is connected with a pressing plate 4, the top of the pressing plate 4 is provided with a through slot matched with the clamping block 10, when the movable plate 2 enters the inside of the detection box 1, the electric push rod 12 is started, the electric push rod 12 pushes the connecting seat 13 and the rotating cylinder 14 to descend, at this time the rotating cylinder 14 extrudes the pressing plate 4, the pressing plate 4 and the movable column 5 move downward, the movable block 7 at the bottom end of the movable column 5 moves downward, when the movable block 7 moves, the first spring 6 pulls the connecting block 8 downward, the connecting strip 9 rotationally connected with the connecting block 8 pulls the clamping block 10 to move inward along the through slot, and the chip is clamped and centered; During the clamping and centering of the clamping block 10, due to the existence of the first spring 6, when the size of the chip changes, for example, the size becomes larger, the clamping block 10 stops when moving to the edge of the chip, and the active end of the electric push rod 12 continues to descend, which causes the first spring 6 to continue to elongate, at this time the clamping force of the clamping block 10 is not too large, which does not damage the chip.

[0022] Further, the top of the pressing plate 4 is provided with a slot 11, the bottom of the rotating cylinder 14 is provided with a plug 28 matched with the slot 11, when the electric push rod 12 descends to fix the chip, the plug 28 at the bottom of the rotating cylinder 14 is inserted into the slot 11 first, and then the pressing plate 4 is extruded for fixation.

[0023] The top of the connecting seat 13 is provided with a rotating mechanism, the rotating mechanism controls the rotation of the rotating cylinder 14, the rotating mechanism comprises a motor 23 and a gear 24, the motor 23 is mounted on the top of the connecting seat 13, the output end of the motor 23 is connected with the gear 24, the inner wall of the rotating cylinder 14 is provided with a gear ring matched with the gear 24, when the electric push rod 12 descends and the clamping block 10 fixes the chip, the motor 23 is started, the output end of the motor 23 drives the gear 24 to rotate, since the gear ring is engaged with the gear 24, the rotating cylinder 14 rotates, since the plug 28 at the bottom of the rotating cylinder 14 is already in the inside of the slot 11, when the rotating cylinder 14 rotates, the rotating seat 3 rotates synchronously, and the internal sample of the chip fixed on the top of the pressing plate 4 is subjected to centrifugal work.

[0024] When the sample inside the chip is centrifuged and analyzed, the repeatedly used chip needs to be cleaned, the inner top wall of the connecting seat 13 is provided with a second spring 18, the bottom end of the second spring 18 is connected with a guide column 19, one end of the guide column 19 is connected with a connecting shell 15, the connecting shell 15 is located at the top of the pressing block 16, the connecting column 17 and the top of the pressing block 16 are connected through an electronic buckle, in the initial state, the connecting column 17 and the pressing block 16 are fixed by the electronic buckle, so when the electric push rod 12 is started, the pressing block 16 is fixed, but when cleaning is needed, the electronic buckle is unlocked, the fixing of the pressing block 16 and the connecting column 17 is released, at this time, the second spring 18 synchronously pops out the guide column 19, the connecting shell 15 and the pressing block 16, and extrudes to the surface of the chip, and the contact position of the guide column 19 and the connecting seat 13 is provided with a damping layer, which can prevent the second spring 18 from popping out too fast; The bottom of the pressing block 16 is provided with a camera 27, which is used for shooting the state of the sample inside the chip, and can also be used for rotating the rotating cylinder 14 and positioning the position of the sewage tank, the top of the connecting seat 13 is respectively provided with a sewage tank 25 and a clean water tank 26, the sewage tank 25 and the clean water tank 26 are respectively connected with a clean water pipe 20 and a sewage pipe 21, one end of the clean water pipe 20 and the sewage pipe 21 respectively penetrates the connecting shell 15 and the pressing block 16, and extends to the bottom of the pressing block 16, a water pump 22 is installed in the middle of the sewage pipe 21, and an electronic valve is installed in the inside of the clean water pipe 20, when the pressing block 16 abuts against the top of the chip under the action of the second spring 18, the clean water pipe 20 and the sewage pipe 21 are respectively inserted into the sample inlet and the sewage tank at the top of the chip, at this time, the electronic valve is opened, the water pump 22 starts, and the cleaning liquid in the clean water tank 26 is sucked into the chip along the clean water pipe 20, and then is sucked into the sewage tank 25 through the sewage pipe 21, through the flowing process of the cleaning liquid, the sample inside the chip is cleaned, and after cleaning, taking out for drying and disinfection work will not pollute the surrounding environment.

[0025] After cleaning, the electric push rod 12 lifts the connecting seat 13 and the rotating cylinder 14 upwards, the pressing block 16 contacts the electronic buckle to fix the pressing block 16 and the connecting column 17.

[0026] The electronic buckle and the electronic valve mentioned in the above structure are prior art, and those skilled in the art can choose appropriate equipment for use, so they are not described in detail, as long as they can play the corresponding role.

[0027] Although the embodiments of the present application have been shown and described, the specific embodiments are only an explanation of the present application, and are not a limitation of the application, and the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

Claims

1. A centrifugal nucleic acid microfluidic device, comprising a detection chamber (1), characterized in that: The detection box (1) is equipped with a movable plate (2) inside. A rotating seat (3) is rotatably mounted on the top of the movable plate (2). A movable column (5) is rotatably mounted inside the rotating seat (3). A movable block (7) is connected to one end of the movable column (5). A first spring (6) is mounted on the top of the movable block (7). A connecting block (8) is connected to the top of the first spring (6). A connecting strip (9) is rotatably connected to the side wall of the connecting block (8). A clamping block (10) is rotatably connected to one end of the connecting strip (9). An electric push rod (12) is installed on the top of the detection box (1). A connecting seat (13) is connected to the output end of the electric push rod (12). A rotating cylinder (14) is installed at the bottom of the connecting seat (13). A rotating mechanism is installed on the top of the connecting seat (13). The rotating mechanism controls the rotating cylinder (14) to rotate.

2. The centrifugal nucleic acid microfluidic device according to claim 1, characterized in that: The top of the movable column (5) is connected to a pressure plate (4), and the top of the pressure plate (4) is provided with a through groove that cooperates with the clamping block (10).

3. The centrifugal nucleic acid microfluidic device according to claim 2, characterized in that: The pressure plate (4) has a slot (11) on its top, and the rotating cylinder (14) has a plug (28) at its bottom that cooperates with the slot (11).

4. The centrifugal nucleic acid microfluidic device according to claim 1, characterized in that: The rotating mechanism includes a motor (23) and a gear (24). The motor (23) is mounted on the top of the connecting seat (13). The output end of the motor (23) is connected to the gear (24). The inner wall of the rotating cylinder (14) is equipped with a gear ring that cooperates with the gear (24).

5. The centrifugal nucleic acid microfluidic device according to claim 1, characterized in that: The inside of the test box (1) is equipped with a chain drive mechanism (29), and the control plate (2) of the chain drive mechanism (29) slides inside and outside the test box (1).

6. The centrifugal nucleic acid microfluidic device according to claim 1, characterized in that: The electric push rod (12) has a connecting column (17) on its side wall. The bottom end of the connecting column (17) passes through the connecting seat (13) and is connected to a pressure block (16).

7. A centrifugal nucleic acid microfluidic device according to claim 6, characterized in that: The inner top wall of the connecting seat (13) is provided with a second spring (18), the bottom end of the second spring (18) is connected to a guide post (19), one end of the guide post (19) is connected to a connecting shell (15), the connecting shell (15) is located on the top of the pressure block (16), and the connecting post (17) is connected to the top of the pressure block (16) by an electronic buckle.

8. A centrifugal nucleic acid microfluidic device according to claim 1, characterized in that: The top of the connecting seat (13) is respectively equipped with a sewage tank (25) and a clean water tank (26). The sewage tank (25) and the clean water tank (26) are respectively connected to a clean water pipe (20) and a sewage pipe (21). One end of the clean water pipe (20) and the sewage pipe (21) passes through the connecting shell (15) and the pressure block (16) respectively, and extends to the bottom of the pressure block (16). A water pump (22) is installed in the middle of the sewage pipe (21).

9. A centrifugal nucleic acid microfluidic device according to claim 6, characterized in that: A camera (27) is installed at the bottom of the pressure block (16).

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