Sampling device for detecting microorganisms in underground water

Abstract

The invention discloses a sampling device for groundwater microorganism detection, and belongs to the technical field of groundwater detection equipment, a sampling rotating mechanism comprises a fixed column, the fixed column is sleeved with a spiral lifting sleeve, a guide block is slidably connected into the spiral lifting sleeve, and a reset spring is fixedly connected between the guide block and the spiral lifting sleeve; a sliding groove is formed in the fixed column and comprises a plurality of inclined lifting grooves and straight moving grooves which are communicated end to end; the upper end of the rotary lifting sleeve is rotatably connected with a sliding sleeve through a fixing sleeve, the inner side wall of the sliding sleeve is fixedly connected with a limiting block which is slidably connected to the side wall of the fixing column, and the side wall of the sliding sleeve is fixedly connected with an L-shaped pressing rod and a sampling mechanism which are rotatably connected with the fixing column. After the sampling rotating mechanism reaches the specified underground depth, the sampling mechanisms can be opened one by one through the rolling depth measuring mechanism, underground water is collected, and the device can improve the remote management capability of the multiple sampling mechanisms.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of groundwater detection equipment, and specifically relates to a sampling device for groundwater microbial detection. BACKGROUND

[0002] Groundwater microbial detection refers to a process of comprehensively analyzing the characteristics such as types, quantity, activity and community structure of microorganisms in groundwater samples by using a series of professional microbiological detection techniques and methods. By collecting groundwater samples, whether pathogenic microorganisms (such as Escherichia coli, Salmonella and other microorganisms that may endanger human health) and various indicator microorganisms (microorganisms used to reflect the degree of groundwater pollution or ecological conditions) exist in the groundwater samples is detected under laboratory conditions by using culture method, molecular biology technology (such as PCR, high-throughput sequencing, etc.) and other means, and then the microbial quality and safety status of the groundwater is evaluated, and whether the groundwater meets the relevant water quality standards is judged, thereby providing a scientific basis for the development and utilization of groundwater, pollution prevention and control, and ecological environment protection, etc.

[0003] A groundwater sampling device for different depths (authorized publication number CN118624295B) for groundwater investigation is disclosed in a Chinese patent, which comprises a ring-shaped frame, a mounting seat, a collection cylinder, a limiting assembly, an adjusting assembly and a pulling frame.

[0004] Most of the existing groundwater collection devices are one-time collection, and cannot collect groundwater at different depths multiple times, so the groundwater at different depths can only be sampled by multiple collection. In order to avoid the pollution of the sampling cylinder to the groundwater collected next time, the collection device also needs to be cleaned, which is inconvenient to operate and low in efficiency. The above-mentioned patent solves the problem of repeated collection for collecting groundwater at different depths, and needs to cooperate with a waterproof push rod to provide upward driving force. The length of the waterproof push rod determines the depth of the collectable groundwater, so a longer waterproof push rod is not suitable for collecting deeper groundwater. In addition, due to the large temperature difference between underground and surface, after the groundwater is sampled, the existing sampling device will cause the metabolic and growth rate of microorganisms in the water sample to change if the groundwater is stored for a long time, thereby affecting the detection result of the microorganisms. Therefore, we propose a sampling device for groundwater microbial detection. SUMMARY

[0005] The present application aims to provide a sampling device for groundwater microbial detection to solve the problems in the background art.

[0006] To achieve the above-mentioned purpose, the present application provides the following technical solution: A sampling device for groundwater microbial detection, comprising a sampling rotating mechanism, further comprising: The fixed column is sleeved with a rotating lifting sleeve, the inside of the rotating lifting sleeve is slidably connected with a guide block, and the guide block is fixedly connected with the rotating lifting sleeve; a sliding groove is opened on the fixed column, the sliding groove comprises a plurality of first and last communication inclined grooves and straight grooves; the upper end of the rotating lifting sleeve is rotatably connected with a sliding sleeve through a fixing sleeve, the inner side wall of the sliding sleeve is fixedly connected with a limiting block which is slidably connected on the side wall of the fixed column, and an L-shaped pressing rod is fixedly connected on the side wall of the sliding sleeve; A sampling mechanism is rotatably connected with the fixed column, comprising a rotating sleeve and a sampling cylinder fixedly connected with the rotating sleeve through a plurality of fixed curved rods, wherein the sampling cylinder sucks water source through negative pressure suction, the inside of the sampling cylinder is slidably connected with a piston, and the piston is fixedly connected with the upper end inner wall of the sampling cylinder through a connecting spring; the upper side wall of the piston is fixedly connected with a pull rod penetrating the upper end of the sampling cylinder, and a plug hole is opened on the pull rod; A limiting mechanism is arranged on the upper end of the sampling cylinder, comprising a limiting cylinder fixedly connected on the upper end of the rotating sleeve, and the pull rod penetrates the limiting cylinder, two fixed cylinders are fixedly connected on the side wall of the limiting cylinder, limiting blocks are fixedly connected on each fixed cylinder, and plug rods slidably connected with the corresponding limiting blocks are inserted on each fixed cylinder; two rotating sleeves are rotatably connected on the fixed cylinder, a pressing plate is fixedly connected between the two rotating sleeves, and torsion springs are fixedly connected between each rotating sleeve and the limiting cylinder; the ends of the two plug rods away from the limiting cylinder are fixedly connected with moving cylinders with inclined grooves opened on the side walls, and sliding blocks fixedly connected with the inner side walls of the rotating sleeves are slidably connected in the inclined grooves; the plug rod is matched with the plug hole; A sealing mechanism is arranged on the lower end of the sampling cylinder, which is used for intercepting and filtering microorganisms and impurities in the sampling process, a prompt block is fixedly connected on the upper end of the pull rod, a filtering mechanism is arranged on the lower end of the sampling cylinder, a winding depth measuring mechanism is arranged on the fixed column.

[0007] As a further scheme of the present application, the sealing mechanism comprises a sealing cover, a drainage one-way valve is inserted on the sealing cover, the sealing cover is fixedly connected with the pull rod through two connecting rods, and a filter screen cylinder is sleeved on the sampling cylinder and fixedly connected on the upper end of the sealing cover.

[0008] As a further scheme of the present application, the inner side wall of the sealing cover is fixedly connected with a semiconductor refrigerating sheet.

[0009] As a further scheme of the present application, the plurality of prompt blocks are arranged in the counterclockwise direction, and the colors of the prompt blocks change from dark to light.

[0010] As a further scheme of the present application, the filtering mechanism comprises an annular block fixedly connected on the lower end of the sampling cylinder, an ultrafiltration screen fixedly arranged on the annular block, and a water inlet one-way valve arranged at the center of the ultrafiltration screen.

[0011] As a further further scheme of the present application, the winding depth measuring mechanism comprises a winding shell, a winding drum is arranged inside the winding shell, the winding drum is rotatably connected with the winding shell through two cylinders, a winding pipe is wound on the winding drum, and a handle is fixedly connected to the side wall of the winding drum.

[0012] As a further further scheme of the present application, a scale line is arranged on the winding pipe.

[0013] As a further further scheme of the present application, a gas cylinder is fixedly connected to the side wall of the winding shell, a gas plug is slidably connected inside the gas cylinder, two limiting rods penetrating through the gas cylinder are fixedly connected to the gas plug, and a threaded rod is rotatably connected to the gas plug and is threadedly connected with the gas cylinder.

[0014] As a further further scheme of the present application, the lower end of the rotating sleeve is connected with the bottom plate through a plurality of transmission rods penetrating through the rotating sleeve.

[0015] As a further further scheme of the present application, a threaded rod is rotatably connected to the bottom plate and is fixedly connected to the lower end of the fixed column, and the winding pipe is in communication with the threaded rod through the fixed column.

[0016] Compared with the prior art, the present application has the following advantages: When the present application is used, the sampling mechanism, the limiting mechanism and the sealing mechanism are cooperated, the sampling mechanism can be opened one by one through the winding depth measuring mechanism after the sampling rotating mechanism reaches the specified underground depth, and the underground water is collected. The device can improve the remote management capability of the plurality of sampling mechanisms, and through the cooperation of the sealing mechanism and the filtering mechanism, most of the microorganisms and small impurities can be intercepted at the ultrafiltration net during the sampling of the underground water, and the temperature of the underground water sampling depth position is continuously maintained constant through the semiconductor refrigeration sheet. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a three-dimensional view of a sampling device for underground water microorganism detection; Figure 2 It is a structure schematic view of the sampling mechanism in the sampling device for underground water microorganism detection; Figure 3 It is a structure schematic view of the sampling rotating mechanism and the sampling mechanism part in the sampling device for underground water microorganism detection; Figure 4 It is a structure schematic view of the limiting mechanism in the sampling device for underground water microorganism detection; Figure 5 It is a structure schematic view of the sliding chute in the sampling device for underground water microorganism detection; Figure 6 It is a structure schematic view of the inside of the sampling cylinder in the sampling device for underground water microorganism detection; Figure 7 It is an explosion view at the limiting mechanism in the sampling device for groundwater microbial detection; Figure 8 It is a structural schematic view at the sealing mechanism in the sampling device for groundwater microbial detection; Figure 9 It is a structural schematic view at the winding depth measuring mechanism in the sampling device for groundwater microbial detection; Figure 10 It is a state view when the sampling mechanism extracts groundwater in the sampling device for groundwater microbial detection.

[0018] In the figure: 1, sampling rotating mechanism; 101, fixed column; 102, rotating sleeve; 103, sliding groove; 1031, inclined lifting groove; 1032, straight moving groove; 104, guide block; 105, return spring; 2, sampling mechanism; 201, rotating sleeve; 202, fixed bent rod; 203, sampling cylinder; 204, piston; 205, pull rod; 206, connecting spring; 3, limiting mechanism; 301, limiting cylinder; 302, fixed cylinder; 303, insertion rod; 304, limiting block; 305, rotating sleeve; 306, moving cylinder; 307, inclined groove; 308, sliding block; 309, pressing plate; 310, torsion spring; 311, insertion hole; 312, fixed sleeve; 313, sliding sleeve; 314, limiting block; 315, L-shaped pressing rod; 4, sealing mechanism; 401, sealing cover; 402, drainage one-way valve; 403, connecting rod; 404, filter screen cylinder; 405, semiconductor refrigeration sheet; 5, prompting block; 6, filtering mechanism; 601, annular block; 602, ultrafiltration screen; 603, water inlet one-way valve; 7, winding depth measuring mechanism; 701, winding shell; 702, winding cylinder; 703, winding pipe; 704, cylinder; 705, handle; 706, air cylinder; 707, air plug; 708, limiting rod; 709, threaded rod; 710, transmission rod; 711, bottom plate. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0020] Embodiment one: please refer to Figures 1 to 10In the embodiment of the application, a sampling device for groundwater microbial detection comprises a sampling rotating mechanism 1. The device needs to meet the requirements of the "Technical Specification for Groundwater Environmental Monitoring" (HJ164-2020) that the sampling equipment does not change the chemical composition of groundwater and the water sample needs to be rinsed 2-3 times before sampling. The sampling rotating mechanism 1 comprises a fixed column 101, which is a plastic cylinder. The fixed column 101 is formed by injection molding, and the inside of the fixed column 101 is reserved with a cavity for placing a winding pipe 703. A rotating sleeve 102 is sleeved on the fixed column 101. A sliding groove 103 is carved on the fixed column 101, which is formed by a mold during the injection molding process of the fixed column 101. The sliding groove 103 comprises a plurality of inclined grooves 1031 with depths from bottom to top from deep to shallow and straight grooves 1032 with depths from bottom to top from shallow to deep. The inclined grooves 1031 and the straight grooves 1032 are in communication at the head and tail, so that the guide block 104 can switch back and forth between the inclined grooves 1031 and the straight grooves 1032 during movement. The angle between the two ends of the inclined grooves 1031 and the center is 90 degrees. The inner wall of the rotating sleeve 102 is slidingly connected with a guide block 104 through a carved mounting groove. The guide block 104 and the rotating sleeve 102 are fixedly connected with a return spring 105 for stretching and contracting the guide block 104. In the initial state, the return spring 105 is located at the upper part of the straight groove 1032. The fixed column 101 is rotationally connected with a sampling mechanism 2 for extracting groundwater. Reference Figure 2 , Figure 3 , Figure 6 and Figure 7The sampling mechanism 2 comprises a rotating sleeve 201 rotatably connected to the fixed column 101 by a mechanical seal, the rotating sleeve 201 is made of stainless steel, the mechanical seal can effectively reduce the impurities in the underground water into the gap between the rotating sleeve 201 and the fixed column 101, improve the durability of the structure, the rotating sleeve 201 is fixedly connected with a plurality of fixed bending rods 202 and a sampling cylinder 203 through welding on the side wall, the sampling cylinder 203 needs to meet the requirements in HJ / T164-2004 “Technical specification for groundwater environmental monitoring” that the microbial sampling container should be sterilized in advance and the sampling process should avoid external microbial pollution, the sampling cylinder 203 is made of two layers of 304 stainless steel, a vacuum interlayer is formed in the middle, which can effectively isolate the external temperature, so that the extracted underground water can be in a constant temperature state in a short time, an exhaust hole is opened at the top of the sampling cylinder 203, when the piston 204 moves, the air in the upper part of the sampling cylinder 203 can enter and exit, the piston 204 is slidably connected in the sampling cylinder 203, the piston 204 is made of hollow stainless steel, which can reduce the weight and has a certain hardness, so that the piston 204 will not deform during use, two rubber rings are fixedly connected on the side wall of the piston 204, which can improve the sealing between the piston 204 and the inner wall of the sampling cylinder 203, a pull rod 205 is fixedly connected on the upper side wall of the piston 204 through welding, and the pull rod 205 penetrates through the upper end of the sampling cylinder 203, a connecting spring 206 for pulling the piston 204 upward is fixedly connected between the piston 204 and the inner wall of the upper end of the sampling cylinder 203, before the underground water is extracted, the connecting spring 206 is in a stretched state, a limiting mechanism 3 for limiting the pull rod 205 is arranged at the upper end of the sampling cylinder 203; Reference Figure 3 , Figure 4 and Figure 7As shown, the limiting mechanism 3 comprises a limiting cylinder 301 welded on the upper end of the rotating sleeve 201, and the pull rod 205 is arranged through the limiting cylinder 301, the side wall of the limiting cylinder 301 is fixedly connected and communicated with two fixed cylinders 302, two plug rods 303 are inserted into the two fixed cylinders 302, the side walls of the two plug rods 303 are slidably connected with the limiting blocks 304 fixedly connected with the fixed cylinders 302, so that the limiting blocks 304 limit the plug rods 303 to avoid rotation of the plug rods 303, the fixed cylinders 302 are rotatably connected with rotating sleeves 305, the ends of the two plug rods 303 away from the limiting cylinder 301 are fixedly connected with moving cylinders 306, the side wall of the moving cylinder 306 is provided with an inclined groove 307, the angle between the two end points of the inclined groove 307 and the center of the moving cylinder 306 is 90 degrees, that is, the maximum rotation angle between the rotating sleeve 305 and the pressing plate 309 is 90 degrees, the sliding block 308 fixedly connected with the inner side wall of the rotating sleeve 305 is slidably connected in the inclined groove 307, when the pressing plate 309 rotates downward, the sliding block 308 can drive the moving cylinder 306 to move through the inclined groove 307, the moving cylinder 306 drives the plug rod 303 to pull out from the insertion hole 311 of the pull rod 205, the pressing plate 309 is fixedly connected between the two rotating sleeves 305, the end of the pressing plate 309 away from the rotating sleeve 305 is upwardly curved to reduce the resetting force of the pressing plate 309, the torsion spring 310 is fixedly connected between the rotating sleeve 305 and the limiting cylinder 301, the end of the rotating sleeve 305 close to the limiting cylinder 301 is provided with an annular piece, the limiting cylinder 301 arranged between the annular piece and the limiting cylinder 301 can reset the pressing plate 309, the insertion hole 311 matched with the plug rod 303 is arranged in the pull rod 205, the insertion hole 311 is two groups, when the pull rod 205 is unlocked and moves upward, the moving cylinder 306 can again push the plug rod 303 into the insertion hole 311 after the pressing plate 309 is reset by the torsion spring 310, so as to complete secondary locking of the pull rod 205, the upper end of the rotating lifting sleeve 102 is rotatably connected with the sliding sleeve 313 through the fixed sleeve 312, the upper end of the rotating lifting sleeve 102 is welded with the fixed sleeve 312, the inner side wall of the sliding sleeve 313 is fixedly connected with the limiting block 314 slidably connected with the side wall of the fixed column 101, the fixed column 101 is provided with the sliding groove matched with the limiting block 314, so that the sliding sleeve 313 can only move up and down in the fixed column 101, the side wall of the sliding sleeve 313 is fixedly connected with the L-shaped pressing rod 315, the lower end of the L-shaped pressing rod 315 corresponds to the pressing plate 309, the lower end of the L-shaped pressing rod 315 is provided with a rotatable rubber cylinder for reducing the friction between the L-shaped pressing rod 315 and the pressing plate 309; When upward force is applied to the rotating lifting sleeve 102, the rotating lifting sleeve 102 moves from the lower part to the upper part of the inclined lifting groove 1031 through the guide block 104. Since the depth of the inclined lifting groove 1031 is from deep to shallow from the lower part to the upper part, after the return spring 105 moves to the upper part of the inclined lifting groove 1031, the return spring 105 is in a compressed state. With the continuous upward movement of the rotating lifting sleeve 102, the guide block 104 slides into the upper part of the straight lifting groove 1032, and at the same time, the rotating lifting sleeve 102 rotates counterclockwise relative to the fixed column 101, so that the rotating lifting sleeve 102 can drive the plurality of sampling mechanisms 2 to rotate 90 degrees through the transmission rod 710 and the rotating sleeve 201. When downward force is applied to the rotating lifting sleeve 102, the rotating lifting sleeve 102 moves from the upper part to the lower part of the straight lifting groove 1032 through the guide block 104. Since the depth of the straight lifting groove 1032 is from shallow to deep from the lower part to the upper part, after the return spring 105 moves to the lower part of the straight lifting groove 1032, the return spring 105 is also in a compressed state. With the continuous downward movement of the rotating lifting sleeve 102, the guide block 104 slides into the lower part of the inclined lifting groove 1031, and at the same time, the rotating lifting sleeve 102 pulls the sliding sleeve 313 downward through the fixed sleeve 312. The sliding sleeve 313 presses the pressing plate 309 through the L-shaped pressing rod 315, so that the pressing plate 309 is flipped to drive the rotating sleeve 305 and the sliding block 308 to rotate. When the sliding block 308 rotates, the moving cylinder 306 moves through the inclined groove 307 to move the moving cylinder 306, so that the moving cylinder 306 pulls out the insertion rod 303. At this time, the pull rod 205 is released from the limiting position, and the piston 204 moves upward under the pulling force of the connecting spring 206 to extract underground water.

[0021] Reference Figure 6 And Figure 8 As shown in the drawings, the sampling cylinder 203 is provided with a sealing mechanism 4 for sealing the lower part of the sampling cylinder 203, the upper end of the pull rod 205 is fixedly connected with a prompt block 5 for prompting the sampling sequence of the staff, the lower end of the sampling cylinder 203 is provided with a filtering mechanism 6 mainly used for intercepting microorganisms, and the fixed column 101 is provided with a winding depth measuring mechanism 7 for measuring the sampling depth and controlling the sampling of the sampling mechanism 2.

[0022] The sealing mechanism 4 comprises a sealing cover 401, a sealing ring is arranged between the sealing cover 401 and the lower end of the sampling cylinder 203, so as to improve the sealing performance between the sealing cover 401 and the sampling cylinder 203, a drainage one-way valve 402 is inserted on the sealing cover 401, and is used to drain excess underground water, when the drainage one-way valve 402 covers the lower end of the sampling cylinder 203, the excess underground water in the sampling cylinder 203 will cause the sealing cover 401 to be unable to tightly press the cover, and the excess underground water in the sampling cylinder 203 can be drained through the drainage one-way valve 402, so as to improve the sealing effect of the sealing cover 401, the sealing cover 401 is fixedly connected with the pull rod 205 through two connecting rods 403, a filter screen cylinder 404 for filtering larger impurities is fixedly connected to the upper end of the sealing cover 401 and is sleeved on the sampling cylinder 203, so as to avoid that larger particle impurities are mixed into the sealing cover 401 and cause the sealing performance between the sealing cover 401 and the bottom of the sampling cylinder 203 to be poor, a semiconductor refrigerating sheet 405 is fixedly connected to the inner wall of the sealing cover 401, a temperature sensor is arranged in the sampling cylinder 203, the temperature at the current sampling depth can be measured through the temperature sensor when the underground water is sampled, after the sampling is completed, the underground water is kept at a constant temperature by the sampling cylinder 203, and the temperature change in the sampling cylinder 203 is continuously monitored through the temperature sensor, when the temperature difference in the sampling cylinder 203 is greater than 2 DEG C, the semiconductor refrigerating sheet 405 and the driving circuit are used for heating or refrigeration, and the temperature is accurately adjusted through a PID control algorithm, so as to maintain the water temperature in a range of target value ± 2 DEG C.

[0023] The plurality of prompt blocks 5 are arranged in a counterclockwise direction, and the color gradually changes from dark to light, so that the sampling sequence of each sampling mechanism 2 can be determined according to the color depth and the rotation direction of the plurality of sampling mechanisms 2 when the underground water is sampled multiple times.

[0024] Reference Figure 8 and Figure 10 As shown in the figure, the filtering mechanism 6 comprises an annular block 601 fixedly connected to the lower end of the sampling cylinder 203, the annular block 601 is designed in an integrated manner with the sampling cylinder 203 and is fixedly screwed in the sampling cylinder 203, so as to facilitate taking out the annular block 601 for microbial detection and adjusting the contact force of the sealing ring, an ultrafiltration screen 602 for intercepting microorganisms is fixedly connected to the annular block 601, the pore size specification of the ultrafiltration screen 602 is 0.45 μm, and a water inlet one-way valve 603 is installed at the center of the ultrafiltration screen 602, the water inlet one-way valve 603 will not be opened when the ultrafiltration screen 602 is permeable, and the water inlet one-way valve 603 can be opened when the ultrafiltration screen 602 is blocked due to too much extraction of small impurities, so as to facilitate extracting more underground water for detection.

[0025] The ultrafiltration screen 602 can replace filter screens with different filtering mesh numbers according to needs, so as to meet different filtering and intercepting effects.

[0026] Example two: please refer toFigure 9 , in combination with the base of embodiment 1, the winding depth measuring mechanism 7 comprises a winding shell 701 integrally pressure cast, the winding shell 701 is made of plastic material, which can effectively reduce the weight and facilitate carrying, the winding shell 701 is internally provided with a winding drum 702, and the winding drum 702 is rotatably connected with the side wall of the winding shell 701 through two cylinders 704, the winding drum 702 is wound with a winding pipe 703, the winding pipe 703 adopts PVC-U hard polyvinyl chloride water pipe, which has high hardness, good compression resistance and low cost and is not easy to be crushed, the side wall of the winding drum 702 is fixedly connected with a handle 705 for driving the winding drum 702 to rotate and make the winding pipe 703 uncoil, the winding pipe 703 is provided with scale lines for observing the sampling depth, the side wall of the winding shell 701 is fixedly connected with an air cylinder 706, the air cylinder 706, the winding pipe 703 and a threaded rod 709 are filled with hydraulic oil, the air cylinder 706 is slidably connected with an air plug 707, the air plug 707 is fixedly connected with two limiting rods 708 penetrating through the air cylinder 706, the limiting rods 708 prevent the air plug 707 from rotating, the air plug 707 is rotatably connected with the threaded rod 709 which is threadedly connected with the air cylinder 706, the lower end of the rotating sleeve 102 is fixedly connected with a bottom plate 711 through a plurality of transmission rods 710, and the plurality of transmission rods 710 penetrate through the rotating sleeve 201, the lower end of the fixed column 101 is fixedly connected with the threaded rod 709, and the threaded rod 709 is rotatably connected with the bottom plate 711, when the threaded rod 709 is extended, the bottom plate 711 can be driven to move downward, so that the bottom plate 711 can exert a force on the rotating sleeve 102 relative to the downward movement of the fixed column 101 through the plurality of transmission rods 710, and the winding pipe 703 penetrates through the fixed column 101 and is communicated with the threaded rod 709.

[0027] The working principle of the present application is as follows: When sampling, the staff first puts the sampling rotating mechanism 1 into the sampling well, slowly rotates the handle 705 to make the winding pipe 703 uncoil, and gradually sinks the sampling rotating mechanism 1 into the water to the sampling depth, then rotates the threaded rod 709 to move the air plug 707, at this time, the hydraulic oil in the air cylinder 706 is delivered to the threaded rod 709 through the winding pipe 703, the threaded rod 709 is extended to drive the bottom plate 711 to move downward, and the bottom plate 711 further exerts a force on the rotating sleeve 102 relative to the downward movement of the fixed column 101 through the plurality of transmission rods 710. At this time, the rotating lifting sleeve 102 is guided by the guide block 104 to move from the upper part of the straight-moving groove 1032 to the lower part. The depth of the straight-moving groove 1032 is shallower in the upper part and deeper in the lower part. The reset spring 105 is in a compressed state when it moves to the lower part of the straight-moving groove 1032. As the rotating lifting sleeve 102 continues to move downward, the guide block 104 slides into the lower part of the inclined lifting groove 1031. At the same time, the rotating lifting sleeve 102 pulls the sliding sleeve 313 downward through the fixed sleeve 312. The sliding sleeve 313 presses the pressing plate 309 through the L-shaped pressure rod 315, causing the pressing plate 309 to flip and drive the rotating sleeve 305 and the sliding block 308 to rotate. When the sliding block 308 rotates, the moving cylinder 306 moves through the inclined groove 307, causing the moving cylinder 306 to pull out the insertion rod 303. The pull rod 205 is released from the limit, and the piston 204 moves upward under the pulling force of the connecting spring 206 to extract underground water. In this process, large particles of impurities are first filtered through the filter screen cylinder 404, and smaller particles and microorganisms are intercepted by the ultrafiltration screen 602. If the ultrafiltration screen 602 is blocked by small impurities, the water inlet one-way valve 603 opens under pressure to extract more underground water for detection. At the same time, the temperature sensor in the sampling cylinder 203 measures the current sampling depth temperature and records it by the single-chip microcomputer when sampling underground water. When the piston 204 moves upward, the sealing cover 401 is lifted to block the lower end of the sampling cylinder 203 through the connecting rod 403. Then the staff reverses the threaded rod 709 to reset the air plug 707. The threaded rod 709 is driven by hydraulic oil to retract and pull the bottom plate 711. The bottom plate 711 drives the guide block 104 in the rotating lifting sleeve 102 to move from the lower part of the inclined lifting groove 1031 to the upper part through multiple transmission rods 710. Because the depth of the inclined lifting groove 1031 is deeper in the lower part and shallower in the upper part, the reset spring 105 is in a compressed state when it moves to the upper part of the inclined lifting groove 1031. Finally, the rotating lifting sleeve 102 continues to move upward, causing the guide block 104 to slide into the upper part of the straight-moving groove 1032. At the same time, the rotating lifting sleeve 102 rotates counterclockwise relative to the fixed column 101, and drives multiple sampling mechanisms 2 to rotate 90 degrees through the transmission rod 710 and the rotating sleeve 201, so that a sampling mechanism 2 can be replaced for the next sampling. Then the staff can continue to rotate the handle 705 to move the sampling rotating mechanism 1 downward to change the depth and continue sampling. When secondary sampling is needed, the staff rotates the threaded rod 709 again, and the above steps are repeated. After sampling is completed, the sampling cylinder 203 maintains a constant temperature for underground water, and continuously monitors the temperature change in the sampling cylinder 203 through the temperature sensor. When the temperature difference in the sampling cylinder 203 exceeds 2℃, the semiconductor refrigeration sheet 405 and the driving circuit are used for heating or refrigeration, and the temperature is accurately adjusted through the PID control algorithm to maintain the water temperature within the target value ±2℃ range.

[0028] The above merely describes preferred embodiments of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can make equivalent replacements or changes within the technical scope disclosed by the present application and according to the technical solutions and inventive concept of the present application, which should be covered within the protection scope of the present application.

Claims

1. A sampling device for detecting microorganisms in groundwater, comprising a sampling rotation mechanism (1), characterized in that, Also includes: A fixed column (101) is fitted with a lifting sleeve (102). A guide block (104) is slidably connected inside the lifting sleeve (102), and a return spring (105) is fixedly connected between the guide block (104) and the lifting sleeve (102). A sliding groove (103) is chiseled on the fixed column (101). The sliding groove (103) includes multiple end-to-end connected inclined grooves (1031) and straight grooves (1032). A sliding sleeve (313) is rotatably connected to the upper end of the lifting sleeve (102) through a fixed sleeve (312). A limiting block (314) is fixedly connected to the inner side wall of the sliding sleeve (313) and slidably connected to the side wall of the fixed column (101). An L-shaped pressure rod (315) is fixedly connected to the side wall of the sliding sleeve (313). The sampling mechanism (2), which is rotatably connected to the fixed column (101), includes a rotating sleeve (201) and a sampling cylinder (203) fixedly connected to the rotating sleeve (201) through multiple fixed bent rods (202), wherein the sampling cylinder (203) draws water from the source by negative pressure suction. A limiting mechanism (3) is provided at the upper end of the sampling cylinder (203), including a limiting cylinder (301) fixedly connected to the upper end of the rotating sleeve (201), and a pull rod (205) passing through the limiting cylinder (301). Two fixed cylinders (302) are fixedly connected to the side wall of the limiting cylinder (301), and each fixed cylinder (302) is fixedly connected to a limit block (304), and each fixed cylinder (302) is inserted with a rod (303) that is slidably connected to the corresponding limit block (304). Two rotating sleeves are rotatably connected to the fixed cylinder (302). A pressing plate (309) is fixedly connected between the two rotating sleeves (305), and a torsion spring (310) is fixedly connected between each rotating sleeve (305) and the limiting cylinder (301); a movable cylinder (306) with a groove (307) on its side wall is fixedly connected to the end of each of the two insert rods (303) away from the limiting cylinder (301), and a slider (308) fixedly connected to the inner side wall of the rotating sleeve (305) is slidably connected inside the groove (307); the insert rod (303) matches the insertion hole (311); The sampling tube (203) is provided with a sealing mechanism (4) at its lower end, which is used to intercept and filter microorganisms and impurities during the sampling process.

2. The sampling device for groundwater microbial detection according to claim 1, characterized in that, The upper end of the pull rod (205) is fixedly connected to a prompting block (5), the lower end of the sampling tube (203) is provided with a filtering mechanism (6), and the fixed column (101) is provided with a winding depth measuring mechanism (7). The sealing mechanism (4) includes a sealing cover (401), a drain check valve (402) is inserted into the sealing cover (401), the sealing cover (401) is fixedly connected to the pull rod (205) through two connecting rods (403), a filter screen cylinder (404) sleeved on the sampling cylinder (203) is fixedly connected to the upper end of the sealing cover (401), and a semiconductor cooling chip (405) is fixedly connected to the inner wall of the sealing cover (401).

3. A sampling device for groundwater microbial detection according to claim 2, characterized in that, A piston (204) is slidably connected inside the sampling cylinder (203), and the piston (204) is fixedly connected to the inner wall of the upper end of the sampling cylinder (203) by a connecting spring (206); a pull rod (205) that penetrates the upper end of the sampling cylinder (203) is fixedly connected to the upper side wall of the piston (204), and an insertion hole (311) is drilled on the pull rod (205).

4. A sampling device for groundwater microbial detection according to claim 2, characterized in that, The colors of the multiple prompt blocks (5) change from dark to light in a counterclockwise direction.

5. A sampling device for detecting microorganisms in groundwater according to claim 2, characterized in that, The filtration mechanism (6) includes an annular block (601) fixedly connected to the lower end of the sampling cylinder (203), an ultrafiltration screen (602) fixedly disposed on the annular block (601), and an inlet check valve (603) disposed at the center of the ultrafiltration screen (602).

6. A sampling device for groundwater microbial detection according to claim 2, characterized in that, The winding depth measuring mechanism (7) includes a winding shell (701), inside which a winding drum (702) is provided, and the winding drum (702) is rotatably connected to the winding shell (701) through two cylinders (704). A winding tube (703) is wound on the winding drum (702), and a handle (705) is fixedly connected to the side wall of the winding drum (702).

7. A sampling device for groundwater microbial detection according to claim 6, characterized in that, The winding tube (703) is provided with scale lines.

8. A sampling device for detecting microorganisms in groundwater according to claim 7, characterized in that, An air cylinder (706) is fixedly connected to the side wall of the winding shell (701). An air plug (707) is slidably connected inside the air cylinder (706). Two limiting rods (708) that pass through the air cylinder (706) are fixedly connected to the air plug (707). A threaded rod (709) that is threadedly connected to the air cylinder (706) is rotatably connected to the air plug (707).

9. A sampling device for detecting microorganisms in groundwater according to claim 8, characterized in that, The lower end of the lifting sleeve (102) passes through the rotating sleeve (201) via multiple transmission rods (710) and is fixedly connected to the base plate (711).

10. A sampling device for detecting microorganisms in groundwater according to claim 9, characterized in that, The lower end of the fixed column (101) is fixedly connected to a threaded rod (709) that is rotatably connected to the base plate (711), and the winding tube (703) passes through the fixed column (101) and communicates with the threaded rod (709).

Images