Enzymolysis device for extracting fructus cannabis active peptide and working method of enzymolysis device

Abstract

The invention discloses an enzymolysis device for extracting fructus cannabis active peptide and a working method thereof, and belongs to the field of active peptide extraction equipment. A housing is included; a stirring device and a limiting device positioned on the bottom surface in the shell are arranged in the shell; the stirring device comprises a stirring rod; the stirring rod is fixedly connected to the side face of the shaft rod, and the stirring rod is sleeved with a movable rod parallel to the shaft rod; the limiting device comprises a base; the base is fixedly connected to the bottom face of the container, and an inner cavity in sliding fit with the movable rod is formed in the upper end face of the base. A supporting column is fixedly connected to the bottom face of the inner cavity, and a disc is fixedly connected to the upper end of the supporting column. An annular channel for the movable rod to slide is reserved between the disc and the inner wall of the inner cavity. According to the sampling device, materials are stirred in multiple directions, the efficiency is guaranteed, and in the sampling process, sampling is conducted through the multiple flow channels with different heights, so that samples are more representative.

Description

TECHNICAL FIELD

[0001] The application relates to an enzyme hydrolysis device for extracting active peptides from cannabis seeds and a working method thereof, and belongs to the field of active peptide extraction equipment. BACKGROUND

[0002] The enzyme hydrolysis device, also known as an enzyme reactor, is a device for providing an optimal reaction environment for enzymes and substrates to efficiently and controllably complete specific biochemical reactions. The core purpose is to utilize the biological catalytic properties of enzymes to decompose macromolecular substances (such as proteins, starches, and celluloses) into small molecular substances (such as peptides, amino acids, and glucose) or synthesize new substances under mild conditions (such as normal temperature, normal pressure, and neutral pH).

[0003] Although the common enzyme reactor on the market is provided with a stirring device, the stirring mode is fixed, the stirring effect is poor, the time consumption is long, and the efficiency is low, so it is necessary to improve the enzyme reactor. SUMMARY

[0004] The application aims to solve the above problems in the background art and provide an enzyme hydrolysis device for extracting active peptides from cannabis seeds and a working method thereof.

[0005] The application achieves the above-mentioned purpose by adopting the following technical solutions:

[0006] An enzyme hydrolysis device for extracting active peptides from cannabis seeds comprises an outer shell, a stirring device inside the outer shell, and a limiting device on the bottom surface of the outer shell inside.

[0007] The stirring device comprises a stirring rod, the stirring rod is fixedly connected to the side surface of a shaft, and a movable rod parallel to the shaft is sleeved on the stirring rod.

[0008] The limiting device comprises a base, the base is fixedly connected to the bottom surface of the container, an inner cavity that slidably cooperates with the movable rod is arranged on the upper end surface of the base, a support is fixedly connected to the bottom surface of the inner cavity, the upper end of the support is fixedly connected with a disc, and an annular channel for the sliding of the movable rod is left between the disc and the inner wall of the inner cavity.

[0009] A working method of an enzyme hydrolysis device for extracting active peptides from cannabis seeds comprises the following steps:

[0010] Step 1: Start the motor, the motor drives the stirring rod to rotate, and the material is stirred;

[0011] Step 2: The stirring rod moves while driving the movable rod to move, and the material is stirred from another direction;

[0012] Step three: when sampling, the air is pumped outwards, the air pressure in the inner cavity of the movable rod is reduced, the sliding plate is moved, the flow channel is communicated with the cavity, the material is moved into the cavity, and under the action of gravity, it is moved downwards to the inner cylinder.Compared with the prior art, the beneficial effects of the present application are: the present application not only stirs the material in multiple directions to ensure efficiency, but also samples by multiple flow channels of different heights to make the sample more representative. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 is a front view of an enzyme hydrolysis device for extracting active peptides from cannabis seeds of the present application;

[0014] Figure 2 is a front view of the shell of an enzyme hydrolysis device for extracting active peptides from cannabis seeds of the present application;

[0015] Figure 3 is Figure 1 a sectional view in the A-A direction;

[0016] Figure 4 is a front view of a stirring device of an enzyme hydrolysis device for extracting active peptides from cannabis seeds of the present application;

[0017] Figure 5 is Figure 4 an enlarged structural schematic view of B;

[0018] Figure 6 is Figure 5 a sectional view in the C-C direction;

[0019] Figure 7 is a front view of a limiting device of an enzyme hydrolysis device for extracting active peptides from cannabis seeds of the present application;

[0020] Figure 8 is a top view of a limiting device of an enzyme hydrolysis device for extracting active peptides from cannabis seeds of the present application;

[0021] Figure 9 is a front view of a sampling device of an enzyme hydrolysis device for extracting active peptides from cannabis seeds of the present application. DETAILED DESCRIPTION

[0022] The technical solutions in the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0023] Specific embodiment one: as Figures 1-9The embodiment is illustrated as an enzyme hydrolysis device for extracting active peptides from Cannabis sativa L. seeds, comprising a shell 1, wherein an agitating device 2 and a limiting device 3 on the bottom surface of the shell 1 are arranged inside the shell 1.

[0024] The agitating device 2 comprises an agitating rod 22 fixedly connected to the side surface of a shaft 21, and a movable rod 25 parallel to the shaft 21 is arranged on the agitating rod 22.

[0025] The limiting device 3 comprises a base 31 fixedly connected to the bottom surface of the container 11, wherein an inner cavity 32 slidably matched with the movable rod 25 is arranged on the upper end surface of the base 31, a support 35 is fixedly connected to the bottom surface of the inner cavity 32, and a disc 36 is fixedly connected to the upper end of the support 35. An annular channel for the sliding of the movable rod 25 is left between the disc 36 and the inner wall of the inner cavity 32.

[0026] The movable rod 25 is provided with a through hole 257 slidably matched with the agitating rod 22, and a plurality of flow channels 256 are arranged on the movable rod 25 and are vertically arranged and obliquely arranged with respect to the through hole 257. The movable rod 25 moves with the agitating rod 22 and presents a different movement track from the agitating rod 22 under the influence of the limiting device 3, so that the agitation is multi-directional and diversified, and the material can be fully mixed in a relatively short time, thereby improving the efficiency.

[0027] The limiting device 3 is eccentric with respect to the container 11, so that the movement track of the movable rod 25 is changed.

[0028] A sealing plate 38 is further arranged on the side surface of the movable rod 25 in the channel for the sliding of the movable rod 25 between the disc 36 and the inner wall of the inner cavity 32. The base 31 and the disc 36 are both provided with a hole 34 through which the shaft 21 passes. A sealing strip is arranged between the outer wall of the disc 36, the inner wall of the inner cavity 32 and the sealing plate 38, so as to separate the container 11 from the inner cavity 32.

[0029] The inner cavity 251 is arranged in the movable rod 25, and a hole 27 is arranged on the bottom side surface of the movable rod 25 to connect the inner cavity 251 and the inner cavity 32. The flow channels 256 are provided with partition plates 252 on the upper and lower sides to separate the flow channels 256 from the inner cavity 251. A connecting frame 255 is fixedly connected between the two partition plates 252, a bidirectional elastic telescopic rod 254 is fixedly connected to the connecting frame 255, and two free ends of the bidirectional elastic telescopic rod 254 are respectively fixedly connected to sliding plates 253 between the two partition plates 252. The movement of the sliding plates 253 controls whether the inner cavity 251 is connected to the inside of the container 11. Meanwhile, the oblique arrangement of the flow channels 256 can change the position of the material in the vertical direction, thereby further enhancing the agitation effect.

[0030] When the bidirectional elastic telescopic rod 254 is contracted to the shortest, the flow channels 256 are not connected to the inner cavity 251.

[0031] The bottom of the container 11 is also provided with a sampling device 4; the sampling device 4 comprises a pump 41; the pump 41 is arranged outside an outer cylinder 42, the inside of the outer cylinder 42 is provided with an inner cylinder 43; the top end of the outer cylinder 42 is threadedly connected with a connecting nozzle 44; the connecting nozzle 44 is fixedly connected with the bottom of the container 11, and the connecting nozzle 44 communicates with the inner cavity 32 through the perforation 33. Through negative pressure, the sliding plate 253 moves reversely, and the bidirectional elastic telescopic rod 254 is stretched, so that the material can flow into the cavity 251 through the gap after moving out of the sliding plate 253.

[0032] The bottom surface of the inner cavity 32 is conical.

[0033] The top of the container 11 is provided with a cover 12, the side of the container 11 is provided with a discharge port 13, the bottom of the container 11 is fixedly connected with a motor 14, and the output shaft of the motor 14 is fixedly connected with the shaft rod 21.

[0034] A working method of an enzyme hydrolysis device for extracting active peptides from cannabis seeds, the working method comprising the following steps:

[0035] Step one: start the motor 14, the motor 14 drives the stirring rod 22 to rotate, and the material is stirred;

[0036] Step two: the stirring rod 22 drives the movable rod 25 to move while moving, and the material is stirred from another direction;

[0037] Step three: when sampling, the pump 41 is used to draw air outward, the air pressure in the cavity 251 of the movable rod 25 is reduced, the sliding plate 253 moves, the flow channel 256 is communicated with the cavity 251, the material enters the cavity 251, and moves downward to the inner cylinder 43 under the action of gravity.

[0038] The working principle of the present application is: when using the device, the motor 14 is started, the motor 14 drives the stirring rod 22 to rotate, and the material is stirred;

[0039] The stirring rod 22 drives the movable rod 25 to move while moving, because the bottom of the movable rod 25 is limited by the channel between the disc 36 and the inner wall of the inner cavity 32, so that it can only move within the range defined by the channel, therefore, while the stirring rod 22 rotates around the shaft rod 21, the movable rod 25 makes a circular motion with the shaft center of the base 31 as the center, because the base 31 is eccentric with the container 11, therefore, while the movable rod 25 makes a circular motion, the movable rod 25 also displaces in the horizontal direction relative to the stirring rod 22, and the material is stirred from another direction;

[0040] When the movable rod 25 moves, part of the material will pass through the inclined flow channel 256 on the movable rod 25, so that the material can also move in the vertical direction when being stirred, further enhancing the stirring effect.

[0041] When sampling, the air is pumped out by the pump 41, the air pressure in the cavity 251 inside the movable rod 25 is reduced, the two sliding plates 253 move reversely under the influence of the air pressure, and the bidirectional elastic telescopic rod 254 is stretched, so that the flow channel 256 is communicated with the cavity 251, the material enters the cavity 251, and moves downward to the inner cavity 32 under the action of gravity, and finally moves to the inner cylinder 43 along the conical bottom surface of the inner cavity 32.

[0042] The movable rod 25 is provided with a plurality of flow channels 256, and the movable rod 25 is parallel to the shaft rod 21, so that when sampling, the materials in the plurality of flow channels 256 represent different depths and different positions, which are mixed in the inner cavity 32 and then flow into the inner cylinder 43, compared with the sample taken at a fixed position in the existing equipment, the sample of the present application is from different depths and different positions, and is more representative.

[0043] When the use is completed, the outer cylinder 42 is disassembled, and the cleaning liquid is introduced into the connecting mouth 44, so that the inner cavity 32 and the cavity 251 can be flushed and cleaned.

[0044] It is obvious for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and the present application can be realized in other forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be regarded as exemplary and non-limiting, the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent conditions of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0045] In addition, it should be understood that although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description manner of the specification is only for the sake of clarity, those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be combined to form other embodiments which can be understood by those skilled in the art.

Claims

1. An enzymatic hydrolysis device for extracting active peptides from hemp seeds, characterized in that: Includes an outer shell (1); the outer shell (1) is provided with a stirring device (2) and a limiting device (3) located on the bottom surface inside the outer shell (1); The stirring device (2) includes a stirring rod (22); the stirring rod (22) is fixedly connected to the side of the shaft (21), and a movable rod (25) parallel to the shaft (21) is sleeved on the stirring rod (22). The limiting device (3) includes a base (31); the base (31) is fixedly connected to the bottom surface of the container (11), and the upper end surface of the base (31) is provided with an inner cavity (32) that slides with the movable rod (25); a support column (35) is fixedly connected to the bottom surface of the inner cavity (32), and a disc (36) is fixedly connected to the upper end of the support column (35); an annular channel for the movable rod (25) to slide is left between the disc (36) and the inner wall of the inner cavity (32).

2. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 1, characterized in that: The movable rod (25) is provided with a through hole (257) that slides with the stirring rod (22), and the movable rod (25) is also provided with a plurality of obliquely arranged flow channels (256) perpendicular to the through hole (257).

3. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 1 or 2, characterized in that: The limiting device (3) is not concentric with the container (11).

4. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 3, characterized in that: The channel between the disc (36) and the inner wall of the inner cavity (32) for the sliding of the movable rod (25) is also provided with a sealing plate (38) fixedly connected to the side of the movable rod (25); the base (31) and the disc (36) are both provided with holes (34) for the shaft rod (21) to pass through.

5. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 4, characterized in that: The movable rod (25) has a cavity (251) inside, and the bottom side of the movable rod (25) has a hole (27) that connects the cavity (251) and the inner cavity (32); the upper and lower sides of the flow channel (256) are provided with partition plates (252) that separate the flow channel (256) from the cavity (251), and a connecting frame (255) is fixedly connected between the two partition plates (252). A bidirectional elastic telescopic rod (254) is fixedly connected on the connecting frame (255), and a sliding plate (253) located between the two partition plates (252) is fixedly connected to the two free ends of the bidirectional elastic telescopic rod (254).

6. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 5, characterized in that: When the bidirectional elastic telescopic rod (254) is retracted to its shortest length, the flow channel (256) and the cavity (251) are not connected.

7. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 5, characterized in that: The bottom of the container (11) is also provided with a sampling device (4); the sampling device (4) includes a pump (41); the pump (41) is located on the outside of the outer cylinder (42), and the inner cylinder (43) is provided inside the outer cylinder (42); the top of the outer cylinder (42) is threadedly connected to the connecting nozzle (44); the connecting nozzle (44) is fixedly connected to the bottom of the container (11), and the connecting nozzle (44) communicates with the inner cavity (32) through the perforation (33).

8. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 7, characterized in that: The bottom surface of the inner cavity (32) is cone-shaped.

9. The enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 8, characterized in that: The container (11) has a cover (12) on top, a discharge port (13) on the side, and a motor (14) fixedly connected to the bottom of the container (11). The output shaft of the motor (14) is fixedly connected to the shaft (21).

10. The working method of the enzymatic hydrolysis device for extracting active peptides from hemp seeds according to claim 9, characterized in that: The working method includes the following steps: Step 1: Start the motor (14). The motor (14) drives the stirring rod (22) to rotate and stir the material. Step 2: The stirring rod (22) moves while driving the movable rod (25) to move, stirring the material from another direction; Step 3: During sampling, the pump (41) draws air outward to reduce the air pressure inside the cavity (251) of the movable rod (25), causing the slide plate (253) to move, thereby connecting the flow channel (256) with the cavity (251), allowing the material to enter the cavity (251) and move downward into the inner cylinder (43) under the action of gravity.

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