Sampling device for experimental animals

Abstract

The invention provides a sampling device for experimental animals, and belongs to the technical field of experimental apparatuses. Comprising a working table and further comprises a sampling assembly arranged on the working table, and the sampling assembly comprises a vertical plate, a sampling needle cylinder arranged on one side of the vertical plate, a clamping part detachably arranged on the vertical plate, a piston rod arranged on the inner side of the sampling needle cylinder and a piston handle fixedly arranged at the top of the piston rod and arranged above the sampling needle cylinder; the four groups of binding components are arranged on the workbench and have the same structure as the clamping part; the longitudinal lifting assembly is arranged on the vertical plate and used for driving the piston rod to move upwards. The piston rod is driven by the electric push rod, so that the accuracy of the sampling amount and the stability of the sampling process are ensured, the problem of hemolysis or blood drawing failure caused by non-uniform manual operation speed is effectively avoided, the sample quality is remarkably improved, and four limbs of an experimental animal can be firmly bound by matching with the four groups of limb binding components; the risk of sampling failure caused by struggling of animals is greatly reduced, and potential injury to operators is effectively avoided.

Description

TECHNICAL FIELD

[0001] The application provides a sampling device for experimental animals, and belongs to the technical field of experimental apparatus. BACKGROUND

[0002] In scientific experiments such as biomedical research, drug development and toxicology evaluation, it is often necessary to collect body fluids (mainly blood) of experimental animals (such as mice, rats, etc.) for subsequent physiological and biochemical index analysis.

[0003] The current sampling operation is usually completed manually by experimental personnel, and this process has many challenges and deficiencies: the sampling operation is mostly completed manually, and when blood is manually drawn, the pulling speed of the piston rod is not easy to control, which may cause hemolysis due to pulling too fast or blood coagulation due to pulling too slowly, the sample quality needs to be improved, and the limbs of the experimental animals are not firmly bound, the experimental animals are bound by simple adhesive tape, and the experimental animals may struggle to break free from the adhesive tape during the sampling process, the risk of sampling failure is relatively high, and the experimental animals that break free may cause potential harm to the operator. Based on this, the application provides a sampling device for experimental animals. SUMMARY

[0004] The technical problem solved by the application is that manual operation speed is uneven, causing hemolysis or blood drawing failure, the sample quality needs to be improved, and the limbs of the experimental animals are not firmly bound, the risk of sampling failure due to animal struggle is relatively high, and the animals may cause potential harm to the operator.

[0005] To solve the technical problem, the technical scheme provided by the application is as follows: a sampling device for experimental animals, comprising a workbench, further comprising: A sampling assembly arranged on the workbench for sampling body fluids of experimental animals, comprising a vertical plate, a sampling needle cylinder arranged on one side of the vertical plate, a clamping part arranged on the vertical plate for clamping the sampling needle cylinder, a piston rod arranged inside the sampling needle cylinder, and a piston handle fixedly arranged on the top of the piston rod and arranged above the sampling needle cylinder; Four limb binding assemblies arranged on the workbench for clamping and binding the limbs of the experimental animals, the limb binding assemblies have the same structure as the clamping part; A longitudinal lifting assembly arranged on the vertical plate for driving the piston rod to move upward, comprising an electric push rod and an L-shaped connecting plate connected to the end of the extension rod of the electric push rod, wherein one side wall of the L-shaped connecting plate is provided with a C-shaped plate arranged on the outside of the piston rod and at the bottom of the piston handle.

[0006] Further, the vertical plate is fixedly arranged on one side of the top of the workbench, the electric push rod is fixedly arranged on the side wall of the vertical plate, and the longitudinal lifting assembly further comprises a pressure sensor fixedly arranged between the bottom of the L-shaped connecting plate and the end of the telescopic rod of the electric push rod, so as to monitor the stress condition of the C-shaped plate.

[0007] Further, the longitudinal lifting assembly further comprises a displacement sensor fixedly arranged on the side wall of the main body of the electric push rod and located below the L-shaped connecting plate, so as to monitor the displacement condition on the C-shaped plate and the L-shaped connecting plate.

[0008] Further, the sampling needle cylinder is made of transparent plastic material, scale lines are arranged on the outer wall of the sampling needle cylinder, and the bottom of the piston rod is provided with a piston head in sliding connection with the inside of the sampling needle cylinder.

[0009] Further, the sampling assembly further comprises a liquid guide tube, the bottom of the sampling needle cylinder is provided with a connecting head in communication with one end of the liquid guide tube, and the other end of the liquid guide tube is provided with a needle head.

[0010] Further, the limb binding assembly and the clamping part each comprise a rectangular frame, a lead screw fixedly arranged on the bottom of the rectangular frame, a butterfly nut in threaded connection with the lead screw, a screw rod in threaded connection with the top of the rectangular frame, a clamping plate in rotary connection with the bottom of the screw rod and in sliding connection with the inner side wall of the rectangular frame, and a groove arranged below the clamping plate is arranged on the inner bottom of the rectangular frame.

[0011] Further, a plurality of adjusting holes for the lead screw to pass through are symmetrically arranged on the two sides of the top of the workbench, and a locking hole for the lead screw to pass through is arranged on the upper portion of the side wall of the vertical plate.

[0012] Further, a hand wheel is arranged on the top of the screw rod and arranged outside the rectangular frame, and a limiting groove matched with the side wall of the clamping plate is symmetrically arranged on the two inner side walls of the rectangular frame.

[0013] Further, limiting baffles are symmetrically arranged on the two sides of the middle portion of the outer wall of the sampling needle cylinder and arranged on the top of the rectangular frame.

[0014] The present application has the following beneficial effects: The electric push rod in the longitudinal lifting assembly drives the piston rod, realizes the automation and standardization of the liquid extraction process, can accurately control the ascending speed and displacement of the piston rod, ensures the accuracy of the sampling amount and the stability of the sampling process, effectively avoids the problems of hemolysis or blood extraction failure caused by uneven manual operation speed, significantly improves the sample quality and the consistency of the operation, and the four sets of limb binding assemblies arranged in combination can firmly bind the four limbs of the experimental animal and stably fix the experimental animal on the workbench, which not only greatly reduces the risk of sampling failure caused by the struggle of the animal, but also effectively avoids the potential harm to the operator, reduces the stress reaction of the animal, and is more in line with the animal welfare ethics. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 Structure diagram of the sampling device for experimental animals Figure 1 .

[0016] Figure 2 Structure diagram of the sampling device for experimental animals Figure 2 .

[0017] Figure 3 Structure diagram of the sampling device for experimental animals Figure 3 .

[0018] Figure 4 Structure diagram of the sampling device for experimental animals Figure 4 .

[0019] Figure 5 Partial enlargement of the sampling device for experimental animals Figure 1 .

[0020] Figure 6 Partial enlargement of the sampling device for experimental animals Figure 2 .

[0021] 1, workbench; 2, sampling assembly; 3, limb clamping assembly; 4, longitudinal lifting assembly; 20, vertical plate; 21, sampling needle cylinder; 22, clamping part; 23, piston rod; 24, piston handle; 25, liquid guide pipe; 26, connecting head; 27, needle head; 28, limiting baffle; 30, rectangular frame; 31, screw rod; 32, butterfly nut; 33, screw rod; 34, clamping plate; 35, groove; 36, adjusting hole; 37, limiting groove; 40, electric push rod; 41, L-shaped connecting plate; 42, C-shaped plate; 43, pressure sensor; 44, displacement sensor. DETAILED DESCRIPTION

[0022] In this specification, the orientation terms such as up, down, left, right, front, back, top, bottom, etc. mentioned or possibly mentioned are defined with respect to its structure, which are relative concepts. Therefore, it is possible to change accordingly according to different positions, different use states; therefore, these or other orientation terms should not be interpreted as restrictive terms.

[0023] The singular forms "a", "said" and "the" used in this specification are intended to include plural forms, unless the context clearly indicates otherwise. It should also be understood that the term "and / or" used herein means and includes any or all possible combinations of one or more associated listed items.

[0024] In order to make the technical problems, technical solutions and beneficial effects to be solved in the present application more clear and explicit, the present application will be further described in detail below in combination with the drawings and embodiments. It should be understood that the embodiments described herein are only used to explain the present application and do not limit the present application.

[0025] The present application will be further described below in combination with the drawings.

[0026] According to the drawings Figure 1 、 4 , 5: the present application provides a sampling device for experimental animals, comprising a workbench 1, further comprising a sampling assembly 2 arranged on the workbench 1, for sampling the body fluid of experimental animals, comprising a vertical plate 20 fixedly arranged on one side of the top of the workbench 1, a sampling needle cylinder 21 arranged on one side of the vertical plate 20, a clamping part 22 arranged on the vertical plate 20 for clamping the sampling needle cylinder 21, a piston rod 23 arranged inside the sampling needle cylinder 21, a piston handle 24 fixedly arranged on the top of the piston rod 23 and arranged above the sampling needle cylinder 21, the sampling assembly 2 further comprises a liquid guide pipe 25, the bottom of the sampling needle cylinder 21 is provided with a connecting head 26 in communication with one end of the liquid guide pipe 25, the other end of the liquid guide pipe 25 is provided with a needle 27, the sampling needle cylinder 21 is made of transparent plastic material, the outer wall of the sampling needle cylinder 21 is provided with scale lines, the bottom of the piston rod 23 is provided with a piston head in sliding connection with the inside of the sampling needle cylinder 21, which is not shown in the figure, for real-time observation of the liquid capacity, the outer wall of the sampling needle cylinder 21 is provided with a limiting baffle 28 arranged on the top of a rectangular frame 30 on both sides of the middle part, which ensures that the same type of sampling needle cylinder 21 replaced each time can be well clamped in the preset initial position; specifically, the needle 27 is inserted into the part of the experimental animal to be sampled, the piston handle 24 is held and lifted, driving the piston rod 23 and the piston to rise, a negative pressure is generated in the sampling needle cylinder 21, so that the body fluid of the experimental animal is sucked into the sampling needle cylinder 21 through the needle 27 to complete the collection.

[0027] As shown in the description accompanying drawings Figure 2 、 4As shown in Figure 5, the workbench 1 also includes four sets of restraint assemblies 3, which are used to clamp and restrain the limbs of the experimental animals to effectively prevent them from struggling during sampling and ensure the safety and stability of the operation. The restraint assemblies 3 have the same structure as the clamping part 22. Both the restraint assemblies 3 and the clamping part 22 include a rectangular frame 30, a lead screw 31 fixedly set at the bottom of the rectangular frame 30, a wing nut 32 threaded to the lead screw 31, a screw 33 threaded to the top of the rectangular frame 30, and a clamping plate 34 rotatably connected to the bottom of the screw 33 and slidably connected to the inner side wall of the rectangular frame 30. The bottom of the rectangular frame 30 is provided with a groove 35 placed below the clamping plate 34. Several adjustment holes 36 for the lead screw 31 to pass through are symmetrically provided on both sides of the top of the workbench 1. The upper part of the side wall of the upright plate 20 is provided with a locking hole for the lead screw 31 to pass through. The top of the screw 33 is provided with a handwheel placed on the outside of the rectangular frame 30. The two inner side walls of the rectangular frame 30 are... Symmetrical limiting grooves 37 that match the sidewalls of clamping plate 34 are provided to limit the clamping plate 34. Specifically, in use, the rectangular frame 30 is pressed tightly against the top of the workbench 3 or the sidewall of the upright plate 20, keeping it horizontal with respect to the ground. The lead screw 31 can be passed through the corresponding hole and then locked and fixed from one side of the workbench 1 or the upright plate 20 by a wing nut 32, so that the position of the limb restraint assembly 3 can be flexibly adjusted while the initial position of the clamping part 22 remains unchanged to accommodate experimental animals of different sizes. When the handwheel is rotated, the screw 33 will drive the clamping plate 34 to move up and down within the rectangular frame 30. When used for limb restraint, the animal's limb can be placed in the rectangular frame 30, and the handwheel is rotated to press down the clamping plate 34, gently clamping the animal's limb between the groove 35 and the clamping plate 34. When used as the clamping part 22, it is used to gently clamp the sampling syringe 21 between the groove 35 and the clamping plate 34.

[0028] As an optional embodiment, those skilled in the art can obtain the following based on their own knowledge and common sense: for the problem of dust and rust on the exposed screw, the dust can be cleaned regularly; for the screw, galvanizing or coating can be used to prevent rust and increase service life; when it is rusted to the point of being unusable, it can be replaced with a new one.

[0029] As per the instruction manual Figure 3 , 4As shown in Figure 6, the assembly also includes a longitudinal lifting component 4 mounted on the upright plate 20 for precisely driving the piston rod 23 upward. This component includes an electric push rod 40 fixedly mounted on one side wall of the upright plate 20 and an L-shaped connecting plate 41 connected to the end of the telescopic rod of the electric push rod 40. One side wall of the L-shaped connecting plate 41 has a C-shaped plate 42 located outside the piston rod 23 and at the bottom of the piston handle 24. The longitudinal lifting component 4 also includes a pressure sensor 43 fixedly mounted between the bottom of the L-shaped connecting plate 41 and the end of the telescopic rod of the electric push rod 40 to monitor the force on the C-shaped plate 42. The longitudinal lifting component 4 also includes a displacement sensor 44 fixedly mounted on one side wall of the main body of the electric push rod 40 and located below the L-shaped connecting plate 41 to monitor the force on the C-shaped plate 42 and the L-shaped connecting plate 42. The displacement of the connecting plate 41 is monitored. Specifically, by activating the electric push rod 40, when the extension rod of the electric push rod 40 moves upward, the C-shaped plate 42 pushes the piston handle 24 upward, thereby causing the piston rod 23 to rise smoothly and at a constant speed. As the piston rod 23 rises, a negative pressure is generated inside the sampling syringe 21, thereby drawing the body fluid of the experimental animal into the sampling syringe 21 through the needle 27 for collection. The pressure sensor 43 is used to monitor the thrust on the C-shaped plate 42 when pushing the piston handle 24 in real time, and the displacement sensor 44 is used to monitor the longitudinal displacement of the L-shaped connecting plate 41 and the C-shaped plate 42 in real time. Through the displacement data, the moving distance of the piston rod 23 can be accurately calculated, and then the amount of body fluid extracted can be calculated to achieve accurate automated fluid extraction.

[0030] The control method of this invention is through a programmable PLC controller. The controller adopts existing mature technology, which is not shown in the figure and will not be described in detail here. The control circuit of the controller can be implemented by those skilled in the art through simple programming. The power supply is also common knowledge in the field. Furthermore, this invention is mainly used to protect mechanical devices, so the control method and circuit connection will not be explained in detail here.

[0031] Working principle Preparation stage: According to the size of the experimental animal, select the appropriate adjustment hole 36 on the workbench 1, install and fix the four sets of ligament assemblies 3, fix the sampling syringe 21 with piston rod 23 on the upright plate 20 through the clamping part 22, and connect the liquid guide tube 25 and the needle 27. Animal fixation: Place the experimental animal in the center of the workbench 1, and place its four limbs in the rectangular frames 30 of the four limb restraint components 3. Rotate the handwheels on each limb restraint component 3 one by one to press down the clamps 34, and gently and firmly fix the animal's limbs between the grooves 35 and the clamps 34.

[0032] Puncture positioning: The operator aligns the needle with the sampling site on the animal and manually performs the puncture to confirm that the needle 27 has entered the blood vessel or other part of the body fluid to be collected; Automated sampling: The electric push rod 40 is started by the programmable PLC controller. The extension rod of the electric push rod begins to extend upward at a constant speed, driving the L-shaped connecting plate 41 and the C-shaped plate 42 to move upward synchronously. The C-shaped plate 42 pushes the piston handle 24 upward, which in turn drives the piston rod 23 to move upward smoothly. At this time, a negative pressure begins to form in the sampling syringe 21, and the animal body fluid is sucked into the syringe through the needle 27 and the guide tube 25. Process monitoring and termination: During the sampling process, the displacement sensor 44 monitors the displacement of the piston rod 23 in real time. When the displacement reaches the preset value, the PLC controller controls the electric push rod 40 to stop running, and the sampling is completed. At the same time, the pressure sensor 43 monitors the thrust throughout the process. If the pressure is abnormal and does not exceed the preset pressure threshold, the PLC controller can automatically control the electric push rod 40 to stop running. Sample collection and animal release: After sampling, remove the sampling syringe 21, replace it with a new sampling syringe 21, piston rod 23, liquid guide tube 25, needle 27 and other disposable equipment, then fix the sampling syringe 21 and carry out a new round of experimental animal body fluid sampling. Finally, after sampling is completed, rotate the handwheel of the restraint assembly 3 in the opposite direction to release the clamp 34 and release the experimental animal.

[0033] In summary, this invention automates and standardizes the liquid extraction process by using the electric push rod 40 in the longitudinal lifting assembly 4 to drive the piston rod 23. The electric push rod 40 can precisely control the rising speed and displacement of the piston rod 23, ensuring the accuracy of the sample volume and the stability of the sampling process. This effectively avoids the problems of hemolysis or blood extraction failure caused by uneven manual operation speed, significantly improving sample quality and operational consistency. Furthermore, the four sets of restraint assemblies 3 can firmly restrain the limbs of the experimental animal and stably fix it on the worktable 1. This not only greatly reduces the risk of sampling failure due to the animal struggling, but also effectively avoids potential harm to the operator, while reducing the animal's stress response, which is more in line with animal welfare ethics.

[0034] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A sampling device for laboratory animals, comprising a worktable, characterized in that, Also includes: The sampling assembly set on the workbench is used to sample body fluids from laboratory animals. It includes a stand, a sampling syringe placed on one side of the stand, a detachable clamping part set on the stand for holding the sampling syringe, a piston rod placed inside the sampling syringe, and a piston handle fixedly set on the top of the piston rod and placed above the sampling syringe. Four sets of limb restraint assemblies are set on the workbench for clamping and restraining the limbs of experimental animals. The limb restraint assemblies have the same structure as the clamping parts. The longitudinal lifting assembly installed on the upright plate is used to drive the piston rod to move upward. It includes an electric push rod and an L-shaped connecting plate connected to the end of the telescopic rod of the electric push rod. A C-shaped plate is provided on one side wall of the L-shaped connecting plate, located outside the piston rod and at the bottom of the piston handle.

2. The sampling device for laboratory animals according to claim 1, characterized in that: The upright plate is fixedly installed on one side of the top of the workbench, and the electric push rod is fixedly installed on one side wall of the upright plate. The longitudinal lifting assembly also includes a pressure sensor fixedly installed between the bottom of the L-shaped connecting plate and the end of the electric push rod telescopic rod to monitor the force on the C-shaped plate.

3. The sampling device for laboratory animals according to claim 1, characterized in that: The longitudinal lifting assembly also includes a displacement sensor fixedly installed on one side wall of the electric push rod body and located below the L-shaped connecting plate, for monitoring the displacement of the C-shaped plate and the L-shaped connecting plate.

4. A sampling device for laboratory animals according to claim 1, characterized in that: The sampling syringe is made of transparent plastic material, and the outer wall of the sampling syringe is provided with scale lines. The bottom of the piston rod is provided with a piston head that is slidably connected to the inside of the sampling syringe.

5. A sampling device for laboratory animals according to claim 1, characterized in that: The sampling assembly also includes a liquid guide tube, and the bottom of the sampling syringe is provided with a connector that communicates with one end of the liquid guide tube, and the other end of the liquid guide tube is provided with a needle.

6. A sampling device for laboratory animals according to claim 1, characterized in that: The limb assembly and clamping part each include a rectangular frame, a lead screw fixedly installed at the bottom of the rectangular frame, a wing nut threaded to the lead screw, a screw threaded to the top of the rectangular frame, and a clamping plate rotatably connected to the bottom of the screw and slidably connected to the inner side wall of the rectangular frame. The bottom of the rectangular frame is provided with a groove placed below the clamping plate.

7. A sampling device for laboratory animals according to claim 6, characterized in that: The workbench has several symmetrical adjustment holes on both sides of the top for lead screws to pass through, and the upper part of the side wall of the vertical plate has locking holes for lead screws to pass through.

8. A sampling device for laboratory animals according to claim 6, characterized in that: The top of the screw is provided with a handwheel located on the outside of the rectangular frame, and the two inner sidewalls of the rectangular frame are symmetrically provided with limiting grooves that match the sidewalls of the clamping plate.

9. A sampling device for laboratory animals according to claim 6, characterized in that: The sampling syringe has symmetrical limiting baffles on both sides of the middle of its outer wall, which are placed on the top of the rectangular frame.

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