Active organ wafer and system thereof

Abstract

The invention discloses an active organ wafer which comprises a cover layer, a substrate layer, a porous membrane, at least one first piezoelectric membrane and a control device. The cover layer is provided with a first upper surface and a first lower surface which are opposite, the first lower surface is provided with a first flow channel, the first upper surface is provided with at least one first opening, the at least one first opening is communicated with the first flow channel, and the two ends of the first flow channel are connected with the first inlet and the first outlet respectively. The base layer is arranged below the cover layer and provided with a second upper surface and a second lower surface which are opposite, the second upper surface is provided with a second flow channel, the two ends of the second flow channel are connected with the second inlet and the second outlet respectively, and part of the first flow channel corresponds to part of the second flow channel. The porous membrane is disposed between the cap layer and the base layer. The at least one first piezoelectric film is sealed in the at least one first opening. And the control device is electrically connected with the at least one first piezoelectric film.

Description

Technical Field

[0001] This invention relates to an organ wafer, and more particularly to an active organ wafer and system capable of actively transporting fluids. Background Technology

[0002] Organ wafers are used to replace traditional animal experiments to simulate the microenvironment of human organs and to culture cells and recreate human tissue structures using microfluidic systems. Because organ wafer cell culture requires the delivery of gases containing carbon dioxide, gases containing particulate drugs, culture media containing specific culture factors, or culture media containing drugs, traditional organ wafers rely on external actuators to input gases from external gas cylinders or culture media from external liquid containers. However, traditional organ wafers and actuators are separate, requiring a actuator to connect to both external gas cylinders and liquid containers for operation, lacking the autonomy of individual organ wafers. Furthermore, in large-scale experiments, it is difficult to adjust the gas or liquid flow rate of individual organ wafers according to their specific needs. Summary of the Invention

[0003] The active organ wafer provided by this invention can autonomously control the flow rate of gas and / or liquid required for experiments without the need for an external driving device.

[0004] To achieve one or more of the above-mentioned objectives or other objectives, an embodiment of the present invention provides an active organ wafer, comprising a capping layer, a substrate layer, a porous membrane, at least one first piezoelectric membrane, and a control device. The capping layer has opposing first upper and lower surfaces, the first lower surface having a first flow channel, and the first upper surface having at least one first opening communicating with the first flow channel, the two ends of the first flow channel being connected to a first inlet and a first outlet, respectively. The substrate layer is disposed below the capping layer, and the substrate layer has opposing second upper and second lower surfaces, the second upper surface facing the first lower surface, the second upper surface having a second flow channel, the two ends of the second flow channel being connected to a second inlet and a second outlet, wherein at least a portion of the first flow channel and a portion of the second flow channel correspond to each other. The porous membrane is disposed between the capping layer and the substrate layer. The first piezoelectric membrane is sealed within the first opening. The control device is electrically connected to the first piezoelectric membrane.

[0005] In one embodiment of the present invention, the above-mentioned active organ wafer further includes at least one first fluid valve, which is disposed at at least one of a first inlet and a first outlet.

[0006] In one embodiment of the present invention, the above-mentioned active organ wafer further includes at least one second fluid valve, which is disposed at least one of a second inlet and a second outlet.

[0007] In one embodiment of the present invention, the first inlet and the first outlet are formed on the first upper surface of the cover layer.

[0008] In one embodiment of the present invention, the cover layer further includes a first sidewall, the first sidewall connecting the first upper surface and the first lower surface, and the first inlet and the first outlet are disposed on the first sidewall.

[0009] In one embodiment of the present invention, the base layer further includes a second sidewall, the second sidewall connecting the second upper surface and the second lower surface, and the second inlet and the second outlet are disposed on the second sidewall.

[0010] In one embodiment of the present invention, the above-mentioned active organ wafer further includes at least one second piezoelectric film, and the second lower surface is provided with at least one second opening, the second opening communicating with a second flow channel, and the second piezoelectric film being sealed in the second opening.

[0011] In one embodiment of the present invention, the control device described above is electrically connected to the second piezoelectric film.

[0012] An embodiment of the present invention provides an active organ wafer, including at least one of the above-described active organ wafers, a storage cavity, and at least one fluid passage. The storage cavity is disposed on the active organ wafer and includes a gas storage cavity with at least one vent. The fluid passage has two opposite ends, one end of which is connected to the vent, and the other end is connected to a first inlet.

[0013] In one embodiment of the present invention, the storage cavity further includes an operating cavity, in which an active organ wafer is disposed. The operating cavity has a plurality of openings, and a first outlet, a second inlet and a second outlet are respectively connected to the plurality of openings.

[0014] In one embodiment of the present invention, there are multiple fluid passages, and the multiple fluid passages are respectively connected to a first outlet, a second inlet and a second outlet to multiple openings.

[0015] In one embodiment of the present invention, the above-mentioned storage cavity further includes a liquid storage cavity, the liquid storage cavity having at least one inlet port connected to a second inlet.

[0016] This invention encapsulates a piezoelectric film within an opening connecting a first flow channel and / or a second flow channel. By controlling the voltage of the piezoelectric film using a control device, the active organ wafer can autonomously regulate the flow rate of the gas and / or liquid required for the experiment, without the need for an external drive device. The active organ wafer system provides a storage cavity suitable for supplying the gas and / or liquid required by the active organ wafer, and the flow rate of the gas and / or liquid is controlled by the active organ wafer.

[0017] To make the above and other objects, features and advantages of the present invention more apparent and understandable, specific embodiments are described below in conjunction with the accompanying drawings. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the appearance of an active organ wafer according to a first embodiment of the present invention.

[0019] Figure 2 This is a three-dimensional exploded view of an active organ wafer according to a first embodiment of the present invention.

[0020] Figure 3 This is a cross-sectional schematic diagram of an active organ wafer according to a first embodiment of the present invention.

[0021] Figure 4 This is a top view schematic diagram of an active organ wafer according to a first embodiment of the present invention.

[0022] Figure 5 This is a cross-sectional schematic diagram of an active organ wafer according to a second embodiment of the present invention.

[0023] Figure 6 This is a cross-sectional schematic diagram of an active organ wafer according to a third embodiment of the present invention.

[0024] Figure 7 This is a cross-sectional schematic diagram of an active organ wafer according to a fourth embodiment of the present invention.

[0025] Figure 8 This is a cross-sectional schematic diagram of an active organ wafer according to a fifth embodiment of the present invention.

[0026] Figure 9 This is a cross-sectional schematic diagram of an active organ wafer system according to a first embodiment of the present invention.

[0027] Figure 10 This is a cross-sectional schematic diagram of an active organ wafer system according to a second embodiment of the present invention.

[0028] Figure 11 This is a cross-sectional schematic diagram of an active organ wafer system according to a third embodiment of the present invention.

[0029] In the attached figures, the following labels are used:

[0030] 100, 100A, 100B, 100C, 100D: Active organ wafers

[0031] 1000, 1000A, 1000B: Active organ-on-a-chip system

[0032] 10, 10A, 10B: Cap layer

[0033] 102: First upper surface

[0034] 104: First lower surface

[0035] 106: First sidewall

[0036] 12: First Stream

[0037] 14: First opening

[0038] 121: First Entrance

[0039] 122: First Exit

[0040] 20, 20A, 20B, 20C: Basal layer

[0041] 202: Second upper surface

[0042] 204: Second lower surface

[0043] 206: Second sidewall

[0044] 22: Second flow channel

[0045] 221: Second Entrance

[0046] 222: Second Exit

[0047] 30: Porous membrane

[0048] 32: Perforation

[0049] 40: First piezoelectric film

[0050] 42: Second piezoelectric film

[0051] 50: Control device

[0052] 60: First fluid valve

[0053] 62: Second fluid valve

[0054] 70, 70A, 70B: Storage chambers

[0055] 702: Spacer

[0056] 72: Stomata

[0057] 74: Opening

[0058] 76: Return vent

[0059] 78: Infusion port

[0060] 80, 80a, 82, 82a: Fluid passage pipes

[0061] R1: Gas storage chamber

[0062] R2: Operating chamber

[0063] R3: Liquid storage chamber Detailed Implementation

[0064] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the present invention.

[0065] Figure 1 This is a schematic diagram of the appearance of an active organ wafer according to a first embodiment of the present invention. Figure 2 This is a three-dimensional exploded view of an active organ wafer according to a first embodiment of the present invention. Figure 3 This is a cross-sectional schematic diagram of an active organ wafer according to a first embodiment of the present invention. Figure 1 , Figure 2 and Figure 3 As shown, the active organ wafer 100 includes a capping layer 10, a base layer 20, a porous membrane 30, a first piezoelectric membrane 40, and a control device 50. The capping layer 10 has a first upper surface 102 and a first lower surface 104 facing each other. The first lower surface 104 is provided with a first flow channel 12, and the first upper surface 102 is provided with a first opening 14, which communicates with the first flow channel 12. The two ends of the first flow channel 12 are respectively connected to a first inlet 121 and a first outlet 122. The base layer 20 is disposed below the capping layer 10. The base layer 20 has a second upper surface 202 and a second lower surface 204 facing each other. The second upper surface 202 is provided with a second flow channel 22, and the two ends of the second flow channel 22 are respectively connected to a second inlet 221 and a second outlet 222. At least a portion of the first flow channel 12 and at least a portion of the second flow channel 22 correspond to each other. A porous membrane 30 is disposed between the capping layer 10 and the base layer 20, and the porous membrane 30 has, for example, multiple perforations 32. A first piezoelectric membrane 40 is sealed within the first opening 14. A control device 50 is electrically connected to the first piezoelectric membrane 40.

[0066] Following the above explanation, Figure 4 This is a top view schematic diagram of an active organ wafer according to a first embodiment of the present invention, which only shows the cover layer 10, the first flow channel 12, the first inlet 121, the first outlet 122, the second flow channel 22, the second inlet 221, and the second outlet 222 to express the correspondence between the first flow channel 12 and the second flow channel 22. Figure 4 As shown, the first flow channel 12 corresponds to a portion of the second flow channel 22 (e.g., the middle section of the second flow channel 22), but is not limited thereto. Specifically, the first flow channel 12 is located on the first lower surface 104 (marked as...). Figure 2 The path formed by the second flow channel 22 and the second flow channel 22 on the second upper surface 202 (marked on) Figure 2 The path formed is designed according to experimental requirements, and the path of part of the second flow channel 22 corresponds to the path of part of the first flow channel 12. The corresponding part of the first flow channel 12 and part of the second flow channel 22 are formed by the porous membrane 30 (marked on Figure 2 and Figure 3 (separated)

[0067] The positions of the first inlet 121 and the first outlet 122 are distributed differently according to the path requirements of the first flow channel 12, and the positions of the second inlet 221 and the second outlet 222 are distributed differently according to the path requirements of the second flow channel 22. In one embodiment, as... Figure 2 and Figure 3 As shown, the first inlet 121 and the first outlet 122 penetrate the first upper surface 102, for example, and the second inlet 221 and the second outlet 222 penetrate the second lower surface 204, for example. Fluid entering the active organ wafer 100 flows into the first channel 12 through the first inlet 121 and exits the first channel 12 through the first outlet 122; fluid flows into the second channel 22 through the second inlet 221 and exits the second channel 22 through the second outlet 222. In one embodiment, as... Figure 3 and Figure 4 As shown, the first entrance 121 and the second entrance 221 may be staggered and not opposite each other, and the first exit 122 and the second exit 222 may be staggered and not opposite each other, but are not limited thereto.

[0068] Please continue reading. Figure 3 As shown, the first opening 14 is formed on the path of the first flow channel 12, that is, the portion of the first flow channel 12 corresponding to the opening range of the first opening 14, to connect the first flow channel 12. The first piezoelectric membrane 40 sealed in the first opening 14 is controlled by the control device 50. When the first piezoelectric membrane continuously receives voltage transmitted from the control device 50, the first piezoelectric membrane 40 will repeatedly bend and vibrate in the direction away from and towards the first flow channel 12. When the first piezoelectric membrane 40 bends in the direction away from the first flow channel 12, the volume of the first flow channel 12 increases, the pressure in the first flow channel 12 decreases, and the fluid entering the active organ chip 100 will enter the first flow channel 12 through the first inlet 121; when the first piezoelectric membrane 40 returns to its original state or bends towards the first flow channel 12, the volume of the first flow channel 12 decreases, the pressure in the first flow channel 12 increases, and the fluid in the first flow channel 12 leaves the first flow channel 12 through the first outlet 122.

[0069] In one embodiment, such as Figure 3As shown, the active organ wafer 100 further includes, for example, a first fluid valve 60, which may be disposed at the first inlet 121 and / or the first outlet 122 to prevent fluid backflow. The first fluid valve 60 may be, for example, flat or arc-shaped. In one embodiment, the number of first fluid valves 60 is, for example, two, with the two first fluid valves 60 respectively disposed at one end of the first inlet 121 adjacent to the first flow channel 12 and the other end of the first outlet 122 away from the first flow channel 12, and the two first fluid valves 60 are adapted to shield the first inlet 121 and the first outlet 122, but are not limited thereto. In one embodiment, the first fluid valve 60 may be, for example, tubular, for insertion into the first inlet 121 and the first outlet 122 respectively.

[0070] Figure 5 This is a cross-sectional schematic diagram of an active organ wafer according to a second embodiment of the present invention. The difference between the active organ wafer 100A and the active organ wafer 100 lies in the arrangement of the first inlet 121, the first outlet 122, the second inlet 221, and the second outlet 222. For example... Figure 5 As shown, the capping layer 10A of the active organ wafer 100A further includes, for example, a first sidewall 106, which connects the first upper surface 102 and the first lower surface 104, and a first inlet 121 and a first outlet 122 are disposed on the first sidewall 106. The base layer 20A of the active organ wafer 100A further includes a second sidewall 206, which connects the second upper surface 202 and the second lower surface 204, and a second inlet 221 and a second outlet 222 are disposed on the second sidewall 206. In one embodiment, as Figure 5 As shown, the first inlet 121 and the first outlet 122 may be disposed opposite to each other on the first side wall 106, but are not limited thereto, and the first inlet 121 and the first outlet 122 may not be opposite to each other; the second inlet 221 and the second outlet 222 may be disposed opposite to each other on the second side wall 206, but are not limited thereto, and the second inlet 221 and the second outlet 222 may not be opposite to each other.

[0071] like Figure 5 As shown, the active organ chip 100A may further include a first fluid valve 60, which may be disposed at the first inlet 121 and / or the first outlet 122 to prevent fluid backflow. The number and structure of the first fluid valves 60 may be the same as or similar to those of the active organ chip 100 in the first embodiment, and will not be described in detail here.

[0072] Figure 6 This is a cross-sectional schematic diagram of an active organ wafer according to a third embodiment of the present invention. The difference between the active organ wafer 100B and the active organ wafer 100A lies in the number of first openings 14 and the configuration of the first inlet 121 and the first outlet 122 in the active organ wafer 100B. In the above... Figure 5In the active organ wafer 100A, the cover layer 10A has a single first opening 14, and the first inlet 121 and the first outlet 122 are disposed on the first sidewall 106; while as Figure 6 As shown, in the active organ chip 100B, the first upper surface 102 of the cover layer 10B has a plurality of first openings 14, and the first inlet 121 and the first outlet 122 are disposed on the first upper surface 102.

[0073] Please continue reading. Figure 6 As shown, the number of first piezoelectric films 40 may be less than or equal to the number of first openings 14. In one embodiment, the number of first piezoelectric films 40 may be a single film. The first piezoelectric film 40 passes through a portion of the cover layer 10B and closes multiple first openings 14. However, this is not the only possibility. In an embodiment not shown, the number of first piezoelectric films 40 may be, for example, multiple films, with multiple first piezoelectric films 40 respectively closing multiple first openings 14. The vibration of the first piezoelectric films 40 is controlled by the control device 50 to adjust the pressure within the first flow channel 12, thereby changing the flow rate of the fluid within the first flow channel 12.

[0074] Furthermore, in the third embodiment of the active organ chip 100B, in addition to including the first fluid valve 60, a second fluid valve 62 may also be included, disposed at the second inlet 221 and / or the second outlet 222. The purpose of providing the first fluid valve 60 and the second fluid valve 62 is to prevent fluid backflow; therefore, the shapes of the first fluid valve 60 and the second fluid valve 62 are not limited to flat or arc-shaped, nor are they limited to pivoting or insertion forms; any form that can prevent fluid backflow falls within the scope of the first fluid valve 60 and the second fluid valve 62. Figure 6 In the active organ wafer 100B shown, the first fluid valve 60 is flat or arc-shaped, and the second fluid valve 62 is tubular, but it is not limited to this. Furthermore, any second fluid valve 62 that can prevent fluid backflow can also be applied to the active organ wafer 100 of the first embodiment and the active organ wafer 100A of the second embodiment.

[0075] Figure 7 This is a cross-sectional schematic diagram of an active organ wafer according to a fourth embodiment of the present invention. The difference between the active organ wafer 100C of the fourth embodiment and the active organ wafer 100A of the second embodiment is that the active organ wafer 100C further includes a second piezoelectric film 42, and a second opening 24 is formed on the second lower surface 204 of the substrate layer 20B. In the above... Figure 5 In the active organ wafer 100A, the second lower surface 204 of the substrate layer 20A does not have any openings or apertures; while... Figure 7 As shown, in the active organ wafer 100C, the second lower surface 204 of the substrate layer 20B is provided with a second opening 24, and the second piezoelectric film 42 is sealed in the second opening 24.

[0076] like Figure 7 As shown, in one embodiment, the opening range of the second opening 24 corresponds at least to a portion of the second flow channel 22. The second piezoelectric membrane 42 is sealed within the second opening 24 and is electrically connected to the control device 50. The opening positions of the first opening 14 and the second opening 24 may correspond or not. When the second piezoelectric membrane 42 receives a voltage transmitted from the control device 50, it repeatedly bends and vibrates in directions away from and towards the second flow channel 22. When the second piezoelectric membrane 42 bends away from the second flow channel 22, the volume of the second flow channel 22 increases, the pressure within the second flow channel 22 decreases, and the fluid entering the active organ wafer 100B enters the second flow channel 22 through the second inlet 221. When the second piezoelectric membrane 42 returns to its original shape or bends towards the second flow channel 22, the volume of the second flow channel 22 decreases, the pressure within the second flow channel 22 increases, and the fluid within the second flow channel 22 exits through the second outlet 222.

[0077] Figure 8 This is a cross-sectional schematic diagram of an active organ wafer according to a fifth embodiment of the present invention. The difference between the active organ wafer 100D of the fifth embodiment and the active organ wafer 100C of the fourth embodiment lies in the number of second openings 24. In the active organ wafer 100C of the fourth embodiment, a single second opening 24 is provided on the second lower surface 204 of the substrate layer 20B as an example. Figure 8 As shown, in the fifth embodiment of the active organ wafer 100D, the second lower surface 204 of the substrate layer 20C may, for example, be provided with a plurality of second openings 24, and the number of second piezoelectric films 42 may be less than or equal to the number of second openings 24. In one embodiment, such as Figure 8 As shown, the number of second piezoelectric films 42 can be a single one. The second piezoelectric film 42 passes through a portion of the substrate layer 20C and closes a plurality of second openings 24. However, it is not limited to this. In an embodiment not shown, the number of second piezoelectric films 42 is, for example, a plurality of them, and the plurality of second piezoelectric films 42 are respectively closed in a plurality of second openings 24.

[0078] According to the above, in the active organ wafer 100 / 100A / 100B / 100C / 100D, when the cover layer 10 / 10A / 10B, the porous membrane 30, and the base layer 20 / 20A / 20B / 20C are sequentially combined, a portion of the first flow channel 12 is opposite to a portion of the second flow channel 22. When the active organ wafer 100 / 100A / 100B / 100C / 100D is used, for example but not limited to, for culturing alveolar cells, gas is introduced into the first flow channel 12, and the alveolar cells are suitable for culturing in the first flow channel 12 and attaching to the porous membrane 30. Culture medium is introduced into the second flow channel 22. Through the perforations 32 of the porous membrane 30 between the cover layer 10 / 10A / 10B and the base layer 20 / 20A / 20B / 20C, the alveolar cells can absorb nutrients from the culture medium in the second flow channel 22.

[0079] Figure 9 This is a cross-sectional schematic diagram of an active organ wafer system according to a first embodiment of the present invention. Figure 5 As shown, the active organ-on-a-chip system 1000 includes a storage cavity 70 and an active organ-on-a-chip 100A, wherein the active organ-on-a-chip 100A can be replaced by the aforementioned active organ-on-a-chip 100, active organ-on-a-chip 100B, active organ-on-a-chip 100C, or active organ-on-a-chip 100D. The structure of the active organ-on-a-chip 100A is as described above and will not be repeated here. The storage cavity 70 is disposed on the active organ-on-a-chip 100A. The storage cavity 70 includes a gas storage cavity R1 and a fluid conduit 80. The gas storage cavity R1 is adapted to store gas to be delivered to the first flow channel 12. The gas contains, for example, but not limited to, particulate drugs. The gas storage cavity R1 is provided with an air hole 72. The fluid conduit 80 has two ends. One end of the fluid conduit 80 is connected to the air hole 72, and the other end of the fluid conduit 80 is connected to the first inlet 121.

[0080] In one embodiment, the active organ-on-a-chip system 1000 may further include a fluid conduit 82 and a fluid conduit 82a. The fluid conduit 82 is connected to a second inlet 221, and the fluid conduit 82a is connected to a second outlet 222 to facilitate connection to an external culture medium-containing vial (not shown) or a waste vial (not shown). In an embodiment not shown, the gas storage chamber R1 may further include an inlet adapted to introduce gas or drug to be introduced into the gas storage chamber R1.

[0081] In one embodiment, the active organ wafer system 1000 may, for example, simultaneously include multiple active organ wafers 100A. The gas storage chamber R1 is provided with multiple vents 72, each vent 72 connected to a fluid conduit 80, and each fluid conduit 80 connected to a first inlet 121 of each of the multiple active organ wafers 100A, but is not limited thereto. When the control device 50 controls the first piezoelectric diaphragm 40 to vibrate, due to the pressure change within the first flow channel 12, gas enters the fluid conduit 80 from the vents 72 of the gas storage chamber R1, passes through the fluid conduit 80 and enters the first flow channel 12 of the multiple active organ wafers 100A via the first inlet 121, and then exits the first flow channel 12 from the first outlet 122 of each of the multiple active organ wafers 100A. In one embodiment, the gas storage chamber R1 is provided with an air hole 72, and the fluid passage 80 is a branch pipe with multiple branch lines. The fluid passage 80 has an input end and multiple output ends. The input end is connected to the air hole 72, and the multiple output ends are respectively connected to the first inlet 121 of multiple active organ wafers 100A, but not limited thereto.

[0082] Figure 10 This is a cross-sectional schematic diagram of a second embodiment of the active organ wafer system of the present invention. Figure 10 As shown, the active organ-on-a-chip system 1000A includes a storage chamber 70A and an active organ-on-a-chip 100A, wherein the active organ-on-a-chip 100A can be replaced by the aforementioned active organ-on-a-chip 100, active organ-on-a-chip 100B, active organ-on-a-chip 100C, or active organ-on-a-chip 100D. The storage chamber 70A of the active organ-on-a-chip system 1000A includes a gas storage chamber R1, an operating chamber R2, and multiple fluid conduits 80, 80a, 82, and 82a. For example, the gas storage chamber R1 is disposed on the operating chamber R2 and separated by a spacer 702, which has vents 72. The operating chamber R2 has multiple openings 74. One end of the fluid conduit 80 is connected to the vent 72, and the other end is connected to a first inlet 121. One end of the fluid conduit 80a is connected to a first outlet 122, and the other end is connected to an opening 74. One end of the fluid conduit 82 is connected to the second inlet 221, and the other end is connected to or passes through the opening 74 to an external solution bottle. One end of the fluid conduit 82a is connected to the second outlet 222, and the other end is connected to or passes through the opening 74 to an external waste liquid bottle.

[0083] Among them, such as Figure 10As shown, the gas storage chamber R1 is provided with a return vent 76, for example. One end of the fluid pipe 80a is connected to the first outlet 122, and the other end is connected to the return vent 76. The gas leaving the first flow channel 12 passes through the fluid pipe 80a connected to the first outlet 122 and is filtered by a filter device. After the impurities in the gas are removed, the gas returns to the gas storage chamber R1 through the return vent 76 connected to the fluid pipe 80a, so that the gas can be recycled.

[0084] Figure 11 This is a cross-sectional schematic diagram of an active organ wafer system according to a third embodiment of the present invention. Figure 11 As shown, the active organ-on-a-chip system 1000B includes a storage chamber 70B and an active organ-on-a-chip 100C, wherein the active organ-on-a-chip 100C can be replaced by an active organ-on-a-chip 100, an active organ-on-a-chip 100A, an active organ-on-a-chip 100B, or an active organ-on-a-chip 100D. The structure of the active organ-on-a-chip 100C is as described above and will not be repeated here. The storage chamber 70B includes a gas storage chamber R1, an operating chamber R2, a liquid storage chamber R3, and multiple fluid conduits 80, 80a, 82, and 82a. The gas storage chamber R1 is, for example, disposed on the operating chamber R2 and separated by a spacer 702, the spacer 702 having at least one vent 72, the operating chamber R2 having multiple openings 74, and the liquid storage chamber R3 having at least one infusion port 78. One end of the fluid passage 80 is connected to the vent 72, and the other end is connected to the first inlet 121; one end of the fluid passage 80a is connected to the first outlet 122, and the other end is connected to the opening 74; one end of the fluid passage 82 is connected to the second inlet 221, and the other end is connected to the infusion port 78; one end of the fluid passage 82a is connected to the second outlet 222, and the other end is connected to the opening 74 or passes through the opening 74 to connect to an external waste liquid bottle.

[0085] In one embodiment of the present invention, an active organ wafer is provided by sealing a first piezoelectric film within a first opening communicating with a first flow channel, and / or sealing a second piezoelectric film within a second opening communicating with a second flow channel, and using a control device to control the vibration of the first piezoelectric film and / or the second piezoelectric film, thereby enabling the active organ wafer to autonomously regulate the flow rate of the gas and / or liquid required for the experiment without the need for an external driving device. The active organ wafer system includes a storage cavity adapted to provide the gas and / or liquid required by the active organ wafer, and can simultaneously supply multiple active organ wafers, with each active organ wafer controlling the flow rate of the gas and / or liquid.

[0086] Although the present invention has been disclosed above by way of embodiments, it is not intended to limit the present invention. Those skilled in the art to which this invention pertains may make some modifications and refinements without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. An active organ chip, characterized in that, Include: A cover layer has a first upper surface and a first lower surface opposite to each other. The first lower surface is provided with a first flow channel, and the first upper surface is provided with at least one first opening. The at least one first opening communicates with the first flow channel, and the two ends of the first flow channel are respectively connected to a first inlet and a first outlet. A base layer is disposed under the cap layer. The base layer has a second upper surface and a second lower surface facing each other. The second upper surface faces the first lower surface. The second upper surface is provided with a second flow channel. The two ends of the second flow channel are respectively connected to a second inlet and a second outlet. At least a portion of the first flow channel and a portion of the second flow channel correspond to each other. A porous membrane is disposed between the capping layer and the substrate layer; At least one first piezoelectric film is sealed within the at least one first opening; and, A control device electrically connected to the at least one first piezoelectric film.

2. The active organ wafer as described in claim 1, characterized in that, It further includes at least one first fluid valve, which is disposed at at least one of the first inlet and the first outlet.

3. The active organ wafer as described in claim 1, characterized in that, It further includes at least one second fluid valve, which is disposed at at least one of the second inlet and the second outlet.

4. The active organ wafer as described in claim 1, characterized in that, in, The first entrance and the first exit are located on the first upper surface of the cover.

5. The active organ wafer as described in claim 1, characterized in that, in, The cover layer further includes a first sidewall, which connects the first upper surface and the first lower surface, and the first inlet and the first outlet are disposed on the first sidewall.

6. The active organ wafer as described in claim 1, characterized in that, in, The base layer further includes a second sidewall that connects the second upper surface and the second lower surface, and the second inlet and the second outlet are disposed on the second sidewall.

7. The active organ wafer as described in claim 1, characterized in that, It further includes at least one second piezoelectric film, the second lower surface having at least one second opening, the at least one second opening communicating with the second flow channel, and the at least one second piezoelectric film being sealed within the second opening.

8. The active organ wafer as described in claim 7, characterized in that, in, The control device is electrically connected to the at least one second piezoelectric film.

9. An active organ wafer system, characterized in that, Include: At least one active organ chip, comprising: A cover layer has a first upper surface and a first lower surface opposite to each other. The first lower surface is provided with a first flow channel, and the first upper surface is provided with at least one first opening. The at least one first opening communicates with the first flow channel, and the two ends of the first flow channel are respectively connected to a first inlet and a first outlet. A base layer is disposed under the cap layer. The base layer has a second upper surface and a second lower surface facing each other. The second upper surface faces the first lower surface. The second upper surface is provided with a second flow channel. The two ends of the second flow channel are respectively connected to a second inlet and a second outlet. At least a portion of the first flow channel and a portion of the second flow channel correspond to each other. A porous membrane is disposed between the capping layer and the substrate layer; At least one first piezoelectric film is sealed within the at least one first opening; and, A control device electrically connected to the at least one first piezoelectric film; A storage cavity is disposed on the at least one active organ wafer, the storage cavity including a gas storage chamber having at least one vent; and At least one fluid passage has opposite ends, one end of which is connected to the at least one vent, and the other end is connected to the first inlet.

10. The active organ wafer system as described in claim 9, characterized in that, in, The storage cavity further includes an operating cavity, in which at least one active organ chip is disposed. The operating cavity has multiple openings, and the first outlet, the second inlet, and the second outlet are respectively connected to the openings.

11. The active organ wafer system as described in claim 10, characterized in that, The number of at least one fluid passage pipes is multiple, and these fluid passage pipes are respectively connected to the first outlet, the second inlet and the second outlet to the openings.

12. The active organ wafer system as described in claim 9, characterized in that, in, The storage cavity further includes a liquid storage cavity, which is provided with at least one inlet, and the at least one inlet is connected to the second inlet.

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