Oligosaccharide and gamma-polyglutamic acid compound and application thereof
By combining oligosaccharides and γ-polyglutamic acid, the biological control problem of wheat scab has been solved, achieving safe and environmentally friendly disease control. It is suitable for the control of wheat scab, powdery mildew, sheath blight and other diseases.
Patent Information
- Application Number
- CN202411117529.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-15
- Publication Date
- 2026-03-03
AI Technical Summary
Existing technologies are ineffective in controlling wheat scab, and the use of chemical pesticides leads to environmental pollution and pathogen resistance. There is a lack of safe and environmentally friendly biological control methods.
Oligosaccharides and γ-polyglutamic acid are combined in different proportions to form soluble liquids, wettable powders, water-dispersible granules, or microcapsules for the prevention and control of diseases such as wheat scab.
It achieves significant biological control effects, long-lasting efficacy, no drug resistance, reduced pesticide residues, conforms to green agricultural development, and is suitable for large-scale promotion.
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Abstract
Description
Technical Field
[0001] This invention relates to a compound agent of oligosaccharide or oligosaccharide derivative and γ-polyglutamic acid and its application in the prevention and control of diseases such as wheat scab. Background Technology
[0002] my country is the world's largest producer and consumer of wheat, with an area of 23.9875 billion hectares. Wheat consumption accounts for more than a quarter of China's total grain production, and the abundance or scarcity of wheat directly affects my country's food security. Fusarium head blight is one of the most destructive wheat diseases. In my country, Fusarium head blight mainly occurs in the middle and lower reaches of the Yangtze River, the winter wheat region of South China, and the eastern part of the spring wheat region of Northeast China. It also occurs in the Yellow River basin and other regions. In recent years, with global warming, Fusarium head blight has also appeared in low-temperature areas such as Northeast and Northwest China, and there is a trend of spreading northward year by year. 20% of wheat-growing areas are affected by Fusarium head blight. In years with an epidemic of Fusarium head blight, yields can be reduced by 20% to 50%. Fusarium head blight not only causes yield reduction, but its main pathogen, *Fusarium graminearum*, can produce various fungal toxins such as trichothecenes and zearalenone, posing a threat to human and animal health. To date, there are no wheat varieties with complete immunity to Fusarium head blight, and production relies on chemical control. The extensive use of chemical pesticides has led to a series of problems, including ecological damage and water pollution. Furthermore, the gradual accumulation of chemical pesticides has fostered resistance in Fusarium head blight fungi. For example, prolonged use of carbendazim alone has resulted in resistance in many areas, posing a risk of failure in controlling Fusarium head blight with existing fungicides like carbendazim. New control technologies and products are urgently needed in production.
[0003] Biological control is a safe, economical, and environmentally friendly method for controlling pests and diseases. However, there are few reports on the biological control of wheat scab, which is currently in the experimental stage. This invention discovers that the combination of oligosaccharides and γ-polyglutamic acid can effectively control scab, demonstrating a positive effect on the biological control of wheat scab. No literature or patent reports have been found on the use of oligosaccharides combined with γ-polyglutamic acid as a novel pesticide for controlling wheat scab.
[0004] γ-Polyglutamic acid (GGA) is a polymer formed by the dehydration condensation of L-glutamic acid and D-glutamic acid. It is a biodegradable polymer with great development potential. Its molecules are linear, containing numerous amide bonds and free carboxyl groups. It possesses unique physicochemical and biological properties, including excellent ductility, flexibility, biocompatibility, adhesion, stability, water absorption, and biodegradability. As a novel natural polymer that is non-toxic to humans and the environment, it is widely used in agriculture, cosmetics, and pharmaceuticals. In agriculture, it is mainly used as a water-retaining agent, fertilizer synergist, and seed germination promoter. Studies have found that GGA has an inhibitory effect on pathogens, but there are currently no reports of its use in the prevention and control of plant diseases.
[0005] Oligosaccharides have been used in plant protection since the 1970s, possessing functions such as inhibiting plant pathogens, inducing plant disease resistance, and promoting plant growth. These oligosaccharides are specifically named oligosaccharide biopesticides, and have advantages such as small relative molecular mass, low toxicity, good water solubility, and strong absorption. Mixing oligosaccharides with fungicides or antibiotics improves the effectiveness of fungicides, reduces the dosage of fungicides or antibiotics, and expands the control range for some fungicides. Combining oligosaccharides with γ-polyglutamic acid can leverage the advantages of both, playing a significant role in the control of plant diseases. However, no literature or patent reports have been found on the use of oligosaccharides combined with γ-polyglutamic acid as a novel pesticide for controlling plant diseases. Summary of the Invention
[0006] The purpose of this invention is to provide a safe and effective compound preparation formed by mixing oligosaccharides and γ-polyglutamic acid in a certain proportion, which can be used to prevent and control diseases such as wheat scab, and its application method.
[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:
[0008] The oligosaccharide compound formulation of the present invention comprises one or more oligosaccharides or oligosaccharide derivatives as active ingredients, and γ-polyglutamic acid or γ-polyglutamic acid salt; wherein the content of oligosaccharides or oligosaccharide derivatives in the compound formulation is 1.25-10%; and the content of γ-polyglutamic acid or γ-polyglutamic acid salt in the compound formulation is 3-20%. The optimal weight ratio of chitosan oligosaccharide to γ-polyglutamic acid is 1:1.2.
[0009] 2. The oligosaccharide in the compound formulation of the present invention is one or more of the following: chitosan oligosaccharide, sodium alginate oligosaccharide, chitin oligosaccharide, oligogalacturonic acid, carrageenan oligosaccharide, and glucosamine oligosaccharide; the oligosaccharide derivative is one or more of the following: oligosaccharide sulfate derivative, oligosaccharide phosphate derivative, oligosaccharide sulfonamide derivative, oligosaccharide acyl isothiocyanate derivative, oligosaccharide phosphorus-containing derivative, oligosaccharide guanidine derivative, oligosaccharide nicotinic acid isothiocyanate derivative, and oligosaccharide-cerium(IV) complex.
[0010] 3. The γ-polyglutamic acid involved in this invention, English name: γ-polyglutamic acid, is mainly γ-polyglutamic acid (γ-PGA, H form), and the γ-polyglutamic acid salt includes Na + Forms of γ-polyglutamate, K + Forms of γ-polyglutamate, NH4 + γ-polyglutamate, Mg 2+ γ-polyglutamate and Ca 2+ γ-Polyglutamate, with a molecular weight ranging from 10 to 2000 kDa. The structure of γ-polyglutamate is as follows:
[0011]
[0012] 4. The oligosaccharide and γ-polyglutamic acid compound combination provided by this invention can be used for the prevention and control of wheat scab, and can also be used for the prevention and control of wheat powdery mildew, sheath blight, rust and other diseases.
[0013] 5. The preferred dosage form of the compound agent described in this invention is a soluble liquid, a wettable powder, a water-dispersible granule, or a microcapsule.
[0014] This invention relates to a compound formulation using oligosaccharides and γ-polyglutamic acid as active components. By mixing these components in different proportions, a synergistic effect is achieved in the control of wheat diseases such as Fusarium head blight, powdery mildew, and sheath blight. The disease control agents involved in this invention are biological pesticides, containing no chemical agents, which aligns with my country's green agricultural development direction and possesses strong market and application prospects.
[0015] The present invention has the following advantages:
[0016] 1. The combination of the present invention is free of chemical pesticides. Both oligosaccharides and γ-polyglutamic acid are green, non-toxic, and biocompatible substances, making them green, environmentally friendly, and environmentally friendly.
[0017] 2. Oligosaccharides are widely available, inexpensive, and easy to process without harm. They can be formulated into soluble liquids, wettable powders, water-dispersible granules, microcapsules, etc., without the need for large amounts of excipients. They are easy to apply and suitable for large-scale promotion and application.
[0018] 3 In addition to directly acting on pathogenic microorganisms, this invention can also regulate plant immunity, induce plant disease resistance, and has a more obvious and longer-lasting effect without producing drug resistance.
[0019] 4. The compound agent in this invention has comparable effects to pure chemical formulations, and can replace chemical pesticides, reducing pesticide residues and solving food safety issues. It can be used in organic and green agricultural production. Detailed Implementation
[0020] The invention will be further explained below with reference to implementation examples.
[0021] Example 1. Inhibitory effect of chitosan oligosaccharide and γ-polyglutamic acid (molecular weight between 10 and 2000 kDa) compound formulation on wheat scab pathogen.
[0022] Chitosan oligosaccharide was prepared into five series of concentrations (0.25, 0.5, 1.0, 2.0, and 4.0 g / L) using sterile water. γ-polyglutamic acid was prepared into five series of concentrations (6.0, 8.0, 10.0, 12.0, and 14.0 g / L) using sterile water. Five additional γ-polyglutamic acid solutions of the same concentration (6.0, 8.0, 10.0, 12.0, and 14.0 g / L) were prepared, and chitosan oligosaccharide was added to each solution to a final concentration of 2.0 g / L to form a homogeneous solution. 1 mL of each prepared solution was added to 9 mL of PDA medium, with sterile water as a blank control. A certain number of mycelial cakes were created from the edge of the colony using a 4 mm punch. After the medium cooled and solidified, the mycelial cakes were placed face down in the center of the drug-containing medium using an inoculation needle. Five replicates were performed for each treatment. The colonies were incubated at 25°C. When the control colonies grew to 2 / 3-3 / 4 of the petri dish diameter, the diameter of each colony was measured with a ruler. The colony diameter was measured using the cross-sectional method, and the average colony diameter and mycelial growth inhibition rate were calculated.
[0023] Inhibition rate = [(Coronavirus diameter of control group - Coronavirus diameter of drug-treated group) / (Coronavirus diameter of control group - Diameter of mycelial cake)] × 100%.
[0024] The mycelial growth inhibition rate was converted into a probability value (y), and the drug concentration was converted into the logarithm of mass concentration (x). A linear regression equation was obtained using the logarithm of mass concentration and probability value regression method: y = a + bx. The median inhibitory concentration (EC50) of each drug against the pathogen was then calculated using this regression equation. 50 The correlation coefficient (R) between the probability value and the logarithm of the mass concentration.
[0025] The experimental results are shown in Table 1. The results indicate that chitosan oligosaccharide and γ-polyglutamic acid have a synergistic effect in inhibiting Fusarium wilt in wheat. (EC) 50 The value is much smaller than that of γ-polyglutamic acid EC. 50 Value, less than chitosan oligosaccharide EC 50 value.
[0026] Table 1: Virulence of chitosan oligosaccharide and γ-polyglutamic acid compound preparations against Fusarium graminearum, the causal agent of wheat blight.
[0027]
[0028] Example 2. Field efficacy of chitosan oligosaccharide and γ-polyglutamic acid (molecular weight 10-2000kD) compound formulation against wheat scab in 2021 (Anhui Province)
[0029] The experiment was conducted in a wheat field in Xintai Village, Wanggang Town, Yingshang County, Fuyang City, Anhui Province. Seven treatments were set up: (1) 90 ml / mu of 8.0% γ-polyglutamic acid aqueous solution; (2) 60 ml / mu of 5% chitosan oligosaccharide aqueous solution; (3) 45 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide aqueous solution (mixed application); (4) 45 ml / mu of 8.0% γ-polyglutamic acid + 60 ml / mu of 5% chitosan oligosaccharide aqueous solution (mixed application); (5) 90 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide aqueous solution (mixed application); (6) 90 ml / mu of 8% tebuconazole aqueous solution; and (7) a water control. Each treatment was replicated three times, with each plot measuring 30 m². 2 The first spraying should be carried out at the early flowering stage of wheat according to the treatment, followed by a second spraying 7 days later, using 30-45L of water per acre. When the wheat is yellow-ripe, the wheat scab disease has stabilized. For each treatment, samples should be taken at 5 points diagonally, with 50 ears per point. The number of diseased ears should be recorded by grade, and the disease incidence rate, disease index, and control effect should be calculated.
[0030] The experimental results are shown in Table 2. The results indicate that all treatments had some control efficacy against wheat scab. The control efficacy of chitosan oligosaccharide and γ-polyglutamic acid alone was 42.23% and 56.21%, respectively. The combined formulations were more effective than the individual applications of the two substances. The treatment with 45 ml of 8.0% γ-polyglutamic acid + 60 ml of 5% chitosan oligosaccharide per mu (60 ml / mu) showed the highest efficacy (73.44%), followed by the treatment with 90 ml of 8.0% γ-polyglutamic acid + 30 ml of 5.0% chitosan oligosaccharide per mu (69.49%). Yield results showed that all treatments yielded higher than the control. The treatment with 45 ml of 8.0% γ-polyglutamic acid + 60 ml of 5% chitosan oligosaccharide per mu (24.44%) showed the highest yield increase, followed by the treatment with 90 ml of 8.0% γ-polyglutamic acid + 30 ml of 5.0% chitosan oligosaccharide per mu (22.60%).
[0031] Table 2: Field control efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulations against wheat scab (2021)
[0032]
[0033] Example 3. Field efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat scab in 2022 (Anhui Province)
[0034] The experiment was conducted in a wheat experimental field of the Academy of Agricultural Sciences in Fuyang City, Anhui Province. Seven treatments were set up: (1) 90 ml / mu of 8.0% γ-polyglutamic acid; (2) 60 ml / mu of 5% chitosan oligosaccharide; (3) 45 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide (mixed application); (4) 45 ml / mu of 8.0% γ-polyglutamic acid + 60 ml / mu of 5% chitosan oligosaccharide (mixed application); (5) 90 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide (mixed application); (6) 90 ml / mu of 8% tebuconazole; and (7) a water control. Each treatment was replicated three times, with each plot covering an area of 40 m². 2 The first spraying should be carried out at the early flowering stage of wheat according to the treatment, followed by a second spraying 7 days later, using 30-45L of water per acre. After the wheat scab disease stabilizes, samples should be taken at 5 points diagonally for each treatment, with 50 ears per point. The number of diseased ears should be recorded by grade, and the disease incidence, disease index, and control effect should be calculated.
[0035] The experimental results are shown in Table 3. The results indicate that all treatments had some control efficacy against wheat scab. The efficacy of chitosan oligosaccharide and γ-polyglutamic acid alone was 43.90% and 46.34%, respectively. The combined formulation was more effective than either substance alone. The treatment with 45 ml of 8.0% γ-polyglutamic acid + 60 ml of 5% chitosan oligosaccharide per mu (60 ml / mu) showed the highest control efficacy (73.17%), followed by the treatment with 90 ml of 8.0% γ-polyglutamic acid + 30 ml of 5.0% chitosan oligosaccharide per mu (90 ml / mu) at 70.73%. Yield results showed that all treatments yielded higher than the control. The treatment with 45 ml of 8.0% γ-polyglutamic acid + 60 ml of 5% chitosan oligosaccharide per mu (90 ml / mu) showed the highest yield increase (20.42%), followed by the treatment with 18.88% increase.
[0036] Table 3: Field control efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulations against wheat scab (2022)
[0037]
[0038] Example 4: Field efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat scab (Shaanxi)
[0039] The experiment was conducted in a wheat field in Xiwang Village, Zhongzhang Town, Jingyang County, Shaanxi Province. The previous crop of the experimental field was corn, and the terrain was flat. A total of 7 treatments were set up: (1) 90 ml / mu of 8.0% γ-polyglutamic acid; (2) 60 ml / mu of 5% chitosan oligosaccharide; (3) 45 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide (mixed application); (4) 45 ml / mu of 8.0% γ-polyglutamic acid + 60 ml / mu of 5% chitosan oligosaccharide (mixed application); (5) 90 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide (mixed application); (6) 50 ml / mu of 8% tebuconazole; (7) water control. Each treatment was replicated 3 times. The first spraying was carried out at the early flowering stage of wheat according to the treatment, and the second spraying was carried out 7 days later. After the wheat scab disease stabilizes, samples are taken at 5 points diagonally for each treatment, with 50 ears at each point. The number of diseased ears is recorded by grade, and the disease incidence, disease index, and control effect are calculated.
[0040] The experimental results are shown in Table 4. The results indicate that all treatments had some control efficacy against wheat scab. The efficacy of chitosan oligosaccharide and γ-polyglutamic acid alone was 45.36% and 53.16%, respectively. The combined formulations were more effective than the individual formulations. The highest control efficacy (89.96%) was achieved with a mixture of 45 ml of 8.0% γ-polyglutamic acid and 60 ml of 5% chitosan oligosaccharide per acre, followed by a mixture of 90 ml of 8.0% γ-polyglutamic acid and 30 ml of 5.0% chitosan oligosaccharide per acre (84.39%). The control efficacy of tebuconazole was 79.03%.
[0041] Table 4: Field control efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulations against wheat scab (Shaanxi 2021)
[0042]
[0043] Example 5: Field efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat scab (Henan)
[0044] The experimental site was located in a wheat field in Gaohuang Village, Hongbu Township, Gushi County, Xinyang City, Henan Province. The previous crop was rice, the soil was clay soil, the field was flat, and irrigation and drainage were convenient. The wheat scab disease occurred frequently in this experimental site. The variety was Fanmai No. 5. A total of 7 treatments were set up in the experiment: (1) 90 ml / mu of 8.0% γ-polyglutamic acid; (2) 60 ml / mu of 5% chitosan oligosaccharide; (3) 45 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide (mixed application); (4) 45 ml / mu of 8.0% γ-polyglutamic acid + 60 ml / mu of 5% chitosan oligosaccharide (mixed application); (5) 90 ml / mu of 8.0% γ-polyglutamic acid + 30 ml / mu of 5% chitosan oligosaccharide (mixed application); (6) 50 ml / mu of 8% tebuconazole; (7) water control. Each treatment was replicated three times. The first spray was applied at the early flowering stage of wheat, and the second spray was applied 7 days later. After the wheat scab disease stabilized, samples were taken at 5 points diagonally from each treatment, with 50 ears per point. The number of diseased ears was recorded by grade, and the disease incidence, disease index, and control effect were calculated.
[0045] The experimental results are shown in Table 5. The results indicate that all treatments had some control efficacy against wheat scab. The efficacy of chitosan oligosaccharide and γ-polyglutamic acid alone was 52.20% and 55.89%, respectively. The combined formulations were more effective than the individual formulations. The highest control efficacy was achieved with a mixture of 45 ml of 8.0% γ-polyglutamic acid and 60 ml of 5% chitosan oligosaccharide per acre (85.11%), followed by a mixture of 90 ml of 8.0% γ-polyglutamic acid and 30 ml of 5.0% chitosan oligosaccharide per acre (75.89%). The control efficacy of tebuconazole was 72.48%.
[0046] Table 5: Field control efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulations against wheat scab (Henan 2022)
[0047]
[0048]
[0049] Example 6: Field efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat powdery mildew.
[0050] The experimental site was a wheat field in Jigusi Village, Qizhen Town, Meixian County, Baoji City, Shaanxi Province. The soil type was Tulou soil, with an organic matter content of 1.1% and a pH of 6.8. Four treatments were set up in the experiment: (1) 90 ml / mu of 8.0% γ-polyglutamic acid; (2) 60 ml / mu of 5% chitosan oligosaccharide; (3) 45 ml / mu of 8.0% γ-polyglutamic acid + 60 ml / mu of 5% chitosan oligosaccharide (mixed application); (4) water control. Each treatment was replicated three times. Spraying was carried out according to the treatment at the wheat jointing stage, and a second spraying was carried out 7 days later. The spraying was carried out evenly using a backpack electric sprayer. Before the application, the disease incidence was investigated. 14 days after the second application, the incidence of wheat powdery mildew was investigated. 20 wheat plants were investigated in each plot. The incidence of disease in the upper 3 functional leaves of each wheat plant was investigated, and the disease severity was recorded. Disease index (disease severity index) efficacy = 1 - [(Disease index before treatment in the control area × Disease index after treatment in the treatment area) ÷ (Disease index after treatment in the control area × Disease index before treatment in the treatment area)] × 100%.
[0051] The experimental results are shown in Table 6. The results indicate that all treatments had some control efficacy against wheat powdery mildew. The efficacy of chitosan oligosaccharide and γ-polyglutamic acid alone was 45.08% and 65.53%, respectively. The combined formulation was more effective than either substance alone; the highest control efficacy (77.96%) was achieved with a mixture of 45 ml of 8.0% γ-polyglutamic acid and 60 ml of 5% chitosan oligosaccharide per acre. These results suggest that the combination of γ-polyglutamic acid and chitosan oligosaccharide has a synergistic effect.
[0052] Table 6: Field control efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat powdery mildew
[0053]
[0054] Example 7: Field efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat sheath blight.
[0055] The experiment was conducted in wheat fields of the Modern Agricultural Service Center in Yingzhou District, Fuyang City, Anhui Province from 2021 to 2022. Four treatments were set up for seed treatment: (1) 3 ml / kg wheat seeds with 8.0% γ-polyglutamic acid; (2) 3 ml / kg wheat seeds with 5% chitosan oligosaccharide; (3) 1.64 ml / kg wheat seeds with 8.0% γ-polyglutamic acid + 1.36 ml / kg wheat seeds with 5% chitosan oligosaccharide (mixed application); (4) water control. Seed treatment was carried out according to the treatment on October 25th when wheat was sown. Four treatments were set up for the spray test: (1) 90 ml / mu with 8.0% γ-polyglutamic acid; (2) 60 ml / mu with 5% chitosan oligosaccharide; (3) 45 ml / mu with 8.0% γ-polyglutamic acid + 60 ml / mu with 5% chitosan oligosaccharide (mixed application); (4) water control. Each treatment was replicated three times, and spraying was carried out according to the type of pesticide applied during the wheat's greening stage. Twenty days after spraying, the incidence of wheat sheath blight was investigated using a diagonal 5-point sampling method, with 5 points surveyed per plot, 10 plants per point, for a total of 50 plants. The incidence was investigated according to the grading standards in the People's Republic of China Agricultural Industry Standard NY / T614-2002 "Specifications for Monitoring and Surveying Wheat Sheath Blight".
[0056] The experimental results are shown in Table 7. The results indicate that all treatments had some control efficacy against wheat sheath blight. The efficacy of chitosan oligosaccharide and γ-polyglutamic acid alone was 37.75% and 43.22%, respectively. The combined formulation was more effective than either substance alone; the combined formulation of γ-polyglutamic acid and chitosan oligosaccharide, when used for seed dressing and spraying, achieved a control efficacy of 71.38%. These results suggest that the combination of γ-polyglutamic acid and chitosan oligosaccharide has a synergistic effect in controlling wheat sheath blight.
[0057] Table 7: Field control efficacy of chitosan oligosaccharide and γ-polyglutamic acid compound formulation against wheat sheath blight
[0058]
Claims
1. An oligosaccharide and γ-polyglutamic acid compound agent, characterized in that: The active ingredients of the compound combination include or consist of two types of compounds, wherein the first type of compounds are one or more oligosaccharides or oligosaccharide derivatives, and the second type of compounds are one or more γ-polyglutamic acid or γ-polyglutamic acid salts.
2. The compounding agent according to claim 1, characterized in that: The mass ratio of the first type of compound to the second type of compound is 1-10:1-15, preferably 1-5:1-5, and more preferably 1:1.2-4.
8.
3. The compounding agent according to claim 1, characterized in that: The oligosaccharide is one or more of the following: chitosan oligosaccharide, sodium alginate oligosaccharide, chitin oligosaccharide, oligogalacturonic acid, carrageenan oligosaccharide, and glucosamine oligosaccharide; the oligosaccharide derivative is one or more of the following: oligosaccharide sulfate derivative, oligosaccharide phosphate derivative, oligosaccharide sulfonamide derivative, oligosaccharide acyl isothiocyanate derivative, oligosaccharide phosphorus-containing derivative, oligosaccharide guanidinyl derivative, oligosaccharide nicotinyl isothiocyanate derivative, and oligosaccharide-cerium(IV) complex.
4. The compounding agent according to claim 1, characterized in that: The γ-polyglutamic acid, English name: γ-polyglutamic acid (γ-PGA, H form), has the structure shown in Formula 1; the γ-polyglutamic acid salt includes Na + Forms of γ-polyglutamate, K + Forms of γ-polyglutamate, NH4 + γ-polyglutamate, Mg 2+ γ-polyglutamate and Ca 2+ One or more of the following γ-polyglutamate salts; molecular weight between 10 and 2000 kDa; 5. The compounding agent according to any one of claims 1-4, characterized in that: The preferred dosage form of the compound is a soluble liquid, a wettable powder, a water-dispersible granule, or a microcapsule.
6. The compounding agent according to claim 5, characterized in that: The first type of compound is present in the compounding agent at a content of 1-10% (preferably 1-3%, more preferably 1-1.25%). The second type of compound is present in a content of 1-15% (preferably 1-6%, more preferably 1-3.6%) in the compounding agent.
7. The application of the compounding agent according to any one of claims 1-6, characterized in that: This compound agent can be used to control one or more of the following diseases of wheat: Fusarium head blight, powdery mildew, or sheath blight.