Dental pulp stem cell collecting device

Abstract

The invention relates to the technical field of cell collection, in particular to a dental pulp stem cell collection device which comprises a supporting seat, a tray and a collection bottle, a cylindrical module is slidably installed in the supporting seat, limiting discs are fixedly installed at the two ends of the cylindrical module, a blade is installed on the cylindrical module, a sliding block and a nut seat are fixedly installed on the tray, and the nut seat is fixedly installed on the supporting seat. A sliding groove and a reciprocating lead screw are correspondingly arranged on the supporting base, an eccentric vibration block is fixedly installed at the output end of the motor, and the eccentric vibration block makes contact with the limiting disc. Wherein a one-way gear is fixedly installed on the reciprocating lead screw, at least two supporting rods are installed in the supporting base in a sliding mode, springs are arranged on the supporting rods in a sleeved mode, one end of each supporting rod abuts against the limiting disc, a rack is fixedly installed at the other end of one supporting rod, and the rack is connected with the one-way gear in a meshed mode. The device has the beneficial effects that the tray is driven by the nut seat to automatically retreat for a small distance along the direction of the sliding chute, so that micro-feeding of a sample is realized, and dental pulp tissues can be uniformly cut up.

Description

Technical Field

[0001] This invention relates to the field of cell collection technology, specifically to a device for collecting dental pulp stem cells. Background Technology

[0002] With the rapid development of regenerative medicine and tissue engineering, stem cells, due to their unique self-renewal capacity and multi-directional differentiation potential, have become a core resource of great strategic value in the biomedical engineering industry. Among various adult stem cells, dental pulp stem cells, due to their wide availability, minimally invasive acquisition, low immunogenicity, strong proliferative capacity, and ability to differentiate into multiple cell types such as osteoblasts, odontoblasts, neuroblasts, and adipocytes, have shown broad application prospects in recent years in fields such as oral regeneration, nerve repair, bone defect reconstruction, and even the treatment of systemic diseases.

[0003] However, despite the significant clinical potential of dental pulp stem cells, the efficient, stable, and sterile extraction from primitive dental pulp tissue remains a key bottleneck restricting their industrial application.

[0004] Traditional methods of processing dental pulp tissue often rely on manual operation. First, the dental pulp tissue needs to be broken up manually using tools, and then cells are separated by enzymatic digestion. However, insufficient manual breaking up will lead to incomplete release of stem cells, significantly reducing cell yield. On the other hand, if the breaking up is excessive or the method is rough, it can easily cause cell membrane damage or even death, affecting subsequent expansion and function. Summary of the Invention

[0005] The purpose of this invention is to provide a device for collecting dental pulp stem cells to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A dental pulp stem cell collection device includes a support base, a tray, and a collection bottle. A cylindrical module is slidably installed within the support base. Limiting discs are fixedly installed at both ends of the cylindrical module. A blade is installed at the end of the cylindrical module near the tray. A slider and a nut seat are fixedly installed on the tray. A sliding groove and a reciprocating screw are correspondingly provided on the support base. A flow tube is fixedly installed on the tray. The collection bottle is sealed to the flow tube. The device also includes a motor. An eccentric vibrator is fixedly installed at the output end of the motor, and the eccentric vibrator contacts the limiting discs. A one-way gear is fixedly installed on the reciprocating screw. At least two support rods are slidably installed within the support base. Springs are sleeved on the support rods. One end of each support rod abuts against the limiting disc. A rack is fixedly installed at the other end of one of the support rods, and the rack meshes with the one-way gear.

[0008] Preferably, the support base includes an L-shaped support plate, a base, and a protective cover. A protective door is hinged to the protective cover. A partition plate is fixedly installed on the L-shaped support plate. The partition plate is arranged vertically along the height direction of the L-shaped support plate. The columnar module is located between the upper and lower partition plates.

[0009] Preferably, the support rod is slidably mounted on the lower partition plate, and the end of the support rod near the limiting plate has a limiting part. The spring is sleeved on the support rod, and the two ends of the spring are fixedly connected to the limiting part and the surface of the lower partition plate, respectively.

[0010] Preferably, the cylindrical module includes a hollow cylinder, a hollow rod is fixedly installed at one end of the hollow cylinder near the lower partition plate, the hollow rod passes through the lower partition plate and is fixedly installed with a knife holder, and also includes a piston installed inside the hollow cylinder, with a push rod fixedly installed on the piston.

[0011] Preferably, the tool holder is provided with a flow guide groove.

[0012] Preferably, a liquid replenishment pipe is fixedly installed on the hollow cylinder, and a one-way valve is fixedly installed inside both the liquid replenishment pipe and the hollow rod.

[0013] Preferably, a metal rod is slidably installed inside the support base, a tension spring is installed on the metal rod, a diagonal rod is fixedly installed on one side of the metal rod, and a diagonal groove adapted to the diagonal rod is opened on the push rod. A coil and a capacitor discharge power supply module are installed inside the support base, the capacitor discharge power supply module is electrically connected to the coil, and conductive contacts are respectively provided on the nut seat and the one-way gear. The conductive contacts are connected in series in the discharge main circuit between the coil and the capacitor discharge power supply module.

[0014] Preferably, the collection bottle includes a bottle body and a bottle cap connected by threads. The bottle cap is provided with an outlet pipe and an inlet pipe. Both the outlet pipe and the inlet pipe are equipped with a one-way valve. The inlet pipe is sealed to the flow pipe.

[0015] Preferably, a filter membrane is installed inside the bottle cap, and the end of the liquid outlet tube located inside the bottle cap passes through the filter membrane.

[0016] Preferably, a positioning point is fixedly installed on the outer edge of the bottle cap, and a positioning groove is provided on the L-shaped support plate.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] 1. By rotating the eccentric vibrating block at high speed, the limiting disk at one end of the cylindrical module is periodically pushed, causing the cylindrical module and the blade at its front end to vibrate at high frequency and micro-amplitude. This reduces the resistance when the blade cuts into the dental pulp sample, which helps to completely and gently cut the dental pulp tissue. At the same time, during the movement of the cylindrical module, the support rod that it is in contact with moves synchronously, which drives the rack fixed at the end of the support rod to drive the one-way gear to rotate. Through the nut seat, the tray is automatically moved back a short distance along the slide groove, realizing micro-feeding of the sample. This helps to uniformly cut the dental pulp tissue, destroy the extracellular matrix, and allow more dental pulp stem cells to be released from the tissue block, thereby increasing the number of collectable stem cells.

[0019] 2. After the tray moves to the maximum path, the pulp sample is cut. The enzyme mixture flowing out from the hollow rod is guided to evenly cover the cutting area along a specific path. On the one hand, the pulp tissue is enzymatically digested in situ, and on the other hand, the tissue debris generated during cutting is flushed away to prevent it from accumulating near the cutting edge. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the structure of each component on the L-shaped support plate of the present invention;

[0022] Figure 3 This is a schematic diagram of the tray mounting structure of the present invention;

[0023] Figure 4 This is a schematic diagram of the cylindrical module structure of the present invention;

[0024] Figure 5 This is a schematic diagram of the internal structure of the hollow cylinder of the present invention;

[0025] Figure 6 This is a schematic diagram of the installation structure of the metal rod, coil, and capacitor discharge power supply module of the present invention.

[0026] Figure 7 This is a schematic diagram of the collection bottle structure of the present invention;

[0027] Figure 8 for Figure 3 Enlarged view of the A-structure;

[0028] Figure 9 for Figure 4 Enlarged view of the B-structure.

[0029] The attached diagram lists the components represented by each number as follows:

[0030] 1. Support base; 101. L-shaped support plate; 1011. Slide groove; 1012. Positioning groove; 102. Base; 103. Protective cover; 104. Protective door; 105. Divider plate;

[0031] 2. Pallet; 201. Flow tube;

[0032] 3. Collection bottle; 301. Bottle body; 302. Bottle cap; 3021. Positioning point; 303. Discharge tube; 304. Inlet tube; 305. Filter membrane;

[0033] 4. Columnar module; 401. Hollow cylinder; 402. Hollow rod; 403. Knife holder; 4031. Guide channel; 404. Piston; 405. Push rod; 4051. Inclined groove; 406. Liquid replenishment pipe;

[0034] 5. Limiting plate; 6. Blade; 7. Slider; 8. Nut seat; 9. Reciprocating lead screw; 10. Motor; 11. Eccentric vibrator; 12. One-way gear;

[0035] 13. Support rod; 1301. Limiting part; 14. Rack; 15. Spring; 16. Metal rod; 17. Tension spring; 18. Diagonal rod; 19. Coil; 20. Capacitor discharge power supply module; 21. Conductive contact. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] Example 1: As Figure 1 - Figure 9 The device shown is for collecting dental pulp stem cells, including a support base 1, a tray 2 and a collection bottle 3, which is used to achieve efficient, sterile and automated collection of dental pulp tissue, thereby providing high-quality original samples for subsequent isolation and culture of dental pulp stem cells.

[0038] The support base 1 forms the main frame of the device, with a cylindrical module 4 slidably mounted inside. Limiting discs 5 are fixedly mounted at both ends of the cylindrical module 4. A blade 6 is mounted on the end of the cylindrical module 4 closest to the tray 2, used for precise cutting of the pulp sample placed on the tray 2. A slider 7 and a nut seat 8 are fixedly mounted on the tray 2. Correspondingly, the support base 1 is provided with a groove 1011 and a reciprocating screw 9. When the reciprocating screw 9 rotates, it drives the tray 2 to reciprocate linearly along the groove 1011 via the nut seat 8, thereby adjusting the position of the pulp sample relative to the blade 6 and ensuring the uniformity of the cutting process. A flow tube 201 is fixedly mounted on the tray 2, with one end open to the surface of the tray 2, used to collect the pulp tissue and accompanying fluid released after cutting. The collection bottle 3 is sealed to the flow tube 201, ensuring the entire collection process is conducted in a closed, sterile environment to prevent external contamination.

[0039] The device also includes a motor 10, which is fixedly mounted on the support base 1. An eccentric vibrating block 11 is fixedly mounted on the output end of the motor 10. The eccentric vibrating block 11 is in contact with the limiting disk 5. In use, the eccentric vibrating block 11 rotates at high speed under the drive of the motor 10. Its outer edge periodically pushes the limiting disk 5 at one end of the cylindrical module 4, thereby causing the cylindrical module 4 and the blade 6 to generate high-frequency micro-amplitude vibration. This vibration helps the blade 6 to cut into the pulp sample more smoothly.

[0040] Furthermore, a one-way gear 12 is fixedly installed on the reciprocating lead screw 9, and at least two support rods 13 are slidably installed in the support base 1, arranged along the axial direction of the cylindrical module 4. A spring 15 is sleeved on the support rod 13, and one end of the support rod 13 abuts against the limiting plate 5 to provide support and guidance when the cylindrical module 4 is reset. A rack 14 is fixedly installed on the other end of one of the support rods 13. The rack 14 is meshed with the one-way gear 12. When the cylindrical module 4 moves forward under the drive of the eccentric vibrator 11, it drives the support rod 13 and the rack 14 to move forward synchronously. The rack 14 pushes the one-way gear 12 to rotate, which in turn drives the reciprocating lead screw 9 to rotate, causing the tray 2 to automatically retreat a small distance. When the cylindrical module 4 retreats under the action of the spring 15, due to the one-way transmission characteristic of the one-way gear 12, the rack 14 slides through the idle stroke without driving the lead screw to reverse, thereby realizing an intermittent automatic feed mechanism for cutting, feeding, and re-cutting.

[0041] It is worth noting that the one-way gear 12 is a ratchet, and its inner ring is fixedly connected to the reciprocating screw 9. The working principle of the ratchet is existing technology and will not be elaborated here.

[0042] After the motor 10 is started, the eccentric vibrating block 11 at its output end rotates at high speed, periodically pushing the limiting disk 5 at one end of the cylindrical module 4, causing the cylindrical module 4 and the blade 6 at its front end to generate high-frequency micro-amplitude vibration, reducing the resistance when the blade 6 cuts into the pulp sample, which helps to completely and gently cut the pulp tissue.

[0043] At the same time, as the cylindrical module 4 moves forward, it pushes the support rod 13 that it abuts to move forward synchronously, which drives the rack 14 fixed to the end of the support rod 13 to drive the one-way gear 12 to rotate. Since the one-way gear 12 is fixedly connected to the reciprocating screw 9, the reciprocating screw 9 rotates accordingly, and drives the tray 2 to automatically retreat a small distance along the direction of the slide groove 1011 through the nut seat 8, so as to realize the micro-feeding of the sample, which helps to uniformly cut the dental pulp tissue and destroy the extracellular matrix, so that more dental pulp stem cells can be released from the tissue block, thereby increasing the number of collectable stem cells.

[0044] After the eccentric oscillator 11 rotates through the pushing phase, the cylindrical module 4 retracts under the restoring force of the spring 15. The one-way gear 12 does not move in the opposite direction with the rack 14 due to its one-way transmission characteristics, thereby preventing the tray 2 from moving back and ensuring that the feeding action is one-way, intermittent and precise.

[0045] The dental pulp tissue and fluid released during the cutting process are directly introduced into the sealed collection bottle 3 through the flow tube 201 on the surface of the tray 2. The entire process is carried out in a closed and sterile environment, which effectively prevents contamination and ensures the quality of subsequent stem cell separation and culture.

[0046] Reference Figure 1 and Figure 2 The support base 1 includes an L-shaped support plate 101, a base 102, and a protective cover 103. The base 102 is equipped with shock-absorbing pads to reduce the overall vibration transmission during operation and improve operational stability. A protective door 104 is hinged to the protective cover 103. The protective door 104 can be opened outward to facilitate the placement of dental pulp tissue, replacement of the blade 6, or internal cleaning and maintenance. A partition plate 105 is fixedly installed on the L-shaped support plate 101. The partition plate 105 is set vertically along the height direction of the L-shaped support plate 101, and the columnar module 4 is located between the upper and lower partition plates 105.

[0047] In this structural layout, the columnar module 4, driven by the motor 10 and the eccentric vibrator 11, slides back and forth between the upper and lower partition plates 105 along the height direction of the L-shaped support plate 101, driving the blade 6 to perform high-frequency micro-amplitude vibration cutting on the pulp sample on the tray 2. At the same time, the tray 2 cooperates with the slide groove 1011 on the L-shaped support plate 101 through the slider 7, and is driven by the reciprocating screw 9 to achieve precise feeding. The entire cutting and collection process is completed within the closed space enclosed by the protective cover 103. The flow tube 201 directly introduces the released pulp tissue into the collection bottle 3, realizing an integrated operation process of sample entry, tissue exit, and full closure.

[0048] Reference Figure 2 and Figure 8The support rod 13 is slidably mounted on the lower partition plate 105. The end of the support rod 13 near the limiting plate 5 has a limiting part 1301. The spring 15 is sleeved on the support rod 13. The two ends of the spring 15 are fixedly connected to the limiting part 1301 and the surface of the lower partition plate 105, respectively.

[0049] When the cylindrical module 4 is pushed forward by the eccentric vibrator 11, the support rod 13 moves accordingly, compressing the spring 15. When the eccentric vibrator 11 no longer applies force to the cylindrical module 4, the spring 15 will push the support rod 13 back to its original position through its elastic restoring force, thereby driving the cylindrical module 4 and the blade 6 on it back to the initial position, completing one cutting cycle.

[0050] In this embodiment, the cylindrical module 4 is not a solid structure, but a composite execution unit composed of multiple functional components. It aims to achieve precise cutting, simultaneous rinsing, and tissue fluid diversion of dental pulp tissue, thereby improving the efficiency and bioactivity of dental pulp stem cell collection. Specifically, referring to... Figure 2 and Figure 4 and Figure 5 and Figure 9 The cylindrical module 4 includes a hollow cylinder 401. A hollow rod 402 is fixedly installed at one end of the hollow cylinder 401 near the lower partition plate 105. The hollow cylinder 401 and the hollow rod 402 are internally connected. The interior of the hollow cylinder 401 is filled with a mixed solution of collagenase and dispersant enzyme. The hollow rod 402 passes through the lower partition plate 105 and is fixedly installed with a knife holder 403. It also includes a piston 404, which is installed inside the hollow cylinder 401. The piston 404 is tightly fitted with the inner wall of the hollow cylinder 401 to form a variable volume sealed cavity. A push rod 405 is fixedly installed on the piston 404.

[0051] Furthermore, the tool holder 403 is provided with a guide groove 4031, which is used to guide the enzyme mixture flowing out from the hollow rod 402 to evenly cover the cutting area along a specific path. On the one hand, it performs in-situ enzymatic hydrolysis of the dental pulp tissue, and on the other hand, it washes away the tissue debris generated during cutting to prevent it from accumulating near the cutting edge.

[0052] To further achieve automatic enzyme replenishment and contamination prevention control, a replenishment pipe 406 is fixedly installed on the hollow cylinder 401 for connecting to an external sterile enzyme storage tank or buffer source. One-way valves are fixedly installed inside both the replenishment pipe 406 and the hollow rod 402. The working surfaces of the one-way valves in the replenishment pipe 406 and the hollow rod 402 are opposite. The one-way valve in the replenishment pipe 406 allows external liquid to flow into the cavity of the hollow cylinder 401 in one direction, while the one-way valve in the hollow rod 402 allows liquid to flow from the hollow cylinder 401 through the hollow rod 402 to the area of ​​the cutter holder 403, but prevents backflow.

[0053] After the device is started, the motor 10 drives the eccentric vibrator 11 to rotate, periodically pushing the cylindrical module 4 to move and generate vibration. The blade 6 cuts into the dental pulp sample, and the push rod 405 drives the piston 404 downward, compressing the inner cavity of the hollow cylinder 401. This causes the pre-stored collagenase and dispersing enzyme mixture to be sprayed out through the one-way valve in the hollow rod 402. The mixture is then evenly applied to the cutting area through the guide groove 4031. The enzyme solution softens the dental pulp tissue in situ at the incision site, promotes the gentle release of stem cells, and works synergistically with mechanical vibration to improve the uniformity of tissue fragmentation. When the piston 404 retracts, a negative pressure is formed inside the hollow cylinder 401, and the one-way valve of the replenishment tube 406 opens, automatically drawing in fresh enzyme solution to replenish the cavity and prepare for the next spray. The released dental pulp tissue and enzyme solution mixture is introduced into the collection bottle 3 through the flow tube 201 on the tray 2, ensuring a completely closed and sterile process.

[0054] To achieve automated release of the enzyme mixture, refer to Figure 2 and Figure 6 A metal rod 16 is slidably installed inside the support base 1. Specifically, the metal rod 16 is mounted on a sliding plate, and a tension spring 17 is installed on the metal rod 16. The two ends of the tension spring 17 are fixed to the metal rod 16 and the sliding plate, respectively. The function of the tension spring 17 is to provide a continuous pulling force to the metal rod 16 towards the protective cover 103. A diagonal rod 18 is fixedly installed on one side of the metal rod 16. A diagonal groove 4051 adapted to the diagonal rod 18 is opened on the push rod 405. A coil 19 and a capacitor discharge power supply module 20 are installed inside the support base 1. The capacitor discharge power supply module 20 is electrically connected to the coil 19, forming a circuit system that can release high-energy pulses instantly. The nut seat 8 and the one-way gear 12 are respectively provided with conductive contacts 21. The conductive contacts 21 are connected in series in the discharge main circuit between the coil 19 and the capacitor discharge power supply module 20. When the tray 2 moves linearly back and forth along the slide groove 1011, the position of the nut seat 8 changes accordingly, thereby causing the conductive contacts 21 to contact or separate, thus affecting the closed or open state of the circuit.

[0055] When the device is in standby mode, the metal rod 16 is held in an initial position due to the action of the tension spring 17. The conductive contact 21 on the nut seat 8 will connect with the corresponding contact on the one-way gear 12 to form a complete discharge circuit. At this time, the capacitor discharge power supply module 20 discharges rapidly to the coil 19, generating an instantaneous strong magnetic field that attracts the metal rod 16, overcoming the tension of the tension spring 17. The inclined rod 18 slides along the inclined groove 4051, pushing the push rod 405 to squeeze the enzyme mixture, thereby achieving the purpose of automatically releasing the enzyme solution after the pulp sample is cut.

[0056] Example 2: Refer to Figure 2 and Figure 7The collection bottle 3 includes a threaded bottle body 301 and a bottle cap 302. The bottle cap 302 is equipped with an outlet pipe 303 and an inlet pipe 304, which are reliably sealed together by a sealing collar to ensure that the pulp tissue suspension discharged from the tray 2 can flow directly and undamaged into the bottle. To prevent backflow or cross-contamination, both the outlet pipe 303 and the inlet pipe 304 are equipped with one-way valves. The inlet pipe 304 is sealed to the flow tube 201. The one-way valve in the inlet pipe 304 allows the tissue suspension to flow into the bottle in one direction, but prevents the liquid in the bottle from flowing back into the flow tube 201. The one-way valve in the outlet pipe 303 remains closed when not in use and only opens when negative or positive pressure is applied externally for controlled drainage.

[0057] Furthermore, a filter membrane 305 is installed inside the bottle cap 302, and one end of the outlet tube 303 inside the bottle cap 302 passes through the filter membrane 305 to perform preliminary screening of the incoming dental pulp tissue suspension: large tissue debris is trapped on the upper side of the membrane, while cell suspension rich in stem cells and small particles can pass through the filter membrane 305 into the lower part of the bottle body 301, which is convenient for subsequent centrifugation or direct culture.

[0058] It is worth noting that a positioning point 3021 is fixedly installed on the outer edge of the bottle cap 302, and a positioning groove 1012 is provided on the L-shaped support plate 101. During installation, the positioning point 3021 is embedded into the positioning groove 1012, which can achieve precise alignment and anti-rotation fixation of the collection bottle 3, ensure reliable sealing connection between the liquid inlet pipe 304 and the flow pipe 201, and prevent the bottle from loosening or falling off due to vibration during operation.

[0059] Working principle:

[0060] After the device is started, the motor 10 drives the eccentric vibrator 11 to rotate at high speed, periodically impacting the limiting disk 5 at one end of the cylindrical module 4. This causes the cylindrical module 4 and its front-end blade 6 to vibrate at high frequency with micro-amplitude. At the same time, as the cylindrical module 4 moves forward, it pushes the support rod 13, which drives the rack 14 at its end to rotate the one-way gear 12. Since the one-way gear 12 is fixedly connected to the reciprocating lead screw 9, the lead screw rotates accordingly and drives the tray 2 to automatically retract a small distance along the slide groove 1011 through the nut seat 8, achieving micro-feeding. When the cylindrical module 4 retracts under the action of the spring 15, the one-way gear 12 does not reverse due to its one-way transmission characteristic, and the tray 2 maintains its current position. This cycle forms an intermittent automatic feeding mechanism of "cutting-feeding-recutting", ensuring that the entire pulp cavity is treated evenly and maximizing the release of stem cells.

[0061] When the tray 2 moves to the preset position, the conductive contact 21 on the nut seat 8 and the one-way gear 12 closes, connecting the discharge circuit composed of the capacitor discharge power supply module 20 and the coil 19. The coil 19 is energized instantaneously to generate a strong magnetic field, attracting the metal rod 16 to move against the tension of the spring 17. The inclined rod 18 on it slides along the inclined groove 4051 of the push rod 405, actively pushing the piston 404, so that the pre-stored collagenase and dispersing enzyme mixture is sprayed out through the one-way valve in the hollow rod 402, and evenly applied to the cutting part through the guide groove 4031. The enzyme solution softens the dental pulp tissue in situ at the cut, promotes the gentle release of stem cells, and improves the uniformity of tissue fragmentation in synergy with mechanical vibration. When the piston 404 retracts, a negative pressure is formed in the hollow cylinder 401, the one-way valve of the replenishment tube 406 opens, and fresh enzyme solution is automatically drawn in to replenish the cavity, preparing for the next spray. The released dental pulp tissue and enzyme solution mixture is introduced into the collection bottle 3 through the flow tube 201 on the tray 2, and the whole process is closed and sterile.

[0062] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0063] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for collecting dental pulp stem cells, characterized in that, The system includes a support base (1), a tray (2), and a collection bottle (3). A cylindrical module (4) is slidably installed inside the support base (1). Limiting plates (5) are fixedly installed at both ends of the cylindrical module (4). A blade (6) is installed at the end of the cylindrical module (4) near the tray (2). A slider (7) and a nut seat (8) are fixedly installed on the tray (2). A sliding groove (1011) and a reciprocating screw (9) are correspondingly provided on the support base (1). A flow tube (201) is fixedly installed on the tray (2). The collection bottle (3) is sealed to the flow tube (201). The system also includes a motor (10). An eccentric vibrating block (11) is fixedly installed at the output end of the motor (10). The eccentric vibrating block (11) is in contact with the limiting plate (5). Among them, a one-way gear (12) is fixedly installed on the reciprocating screw (9), and at least two support rods (13) are slidably installed in the support base (1). A spring (15) is sleeved on the support rod (13). One end of the support rod (13) abuts against the limiting plate (5). A rack (14) is fixedly installed on the other end of one of the support rods (13). The rack (14) meshes with the one-way gear (12).

2. The device for collecting dental pulp stem cells according to claim 1, characterized in that: The support base (1) includes an L-shaped support plate (101), a base (102), and a protective cover (103). A protective door (104) is hinged to the protective cover (103). A partition plate (105) is fixedly installed on the L-shaped support plate (101). The partition plate (105) is arranged vertically along the height direction of the L-shaped support plate (101). The columnar module (4) is located between the upper and lower partition plates (105).

3. The device for collecting dental pulp stem cells according to claim 2, characterized in that: The support rod (13) is slidably mounted on the lower partition plate (105). The end of the support rod (13) near the limiting plate (5) has a limiting part (1301). The spring (15) is sleeved on the support rod (13). The two ends of the spring (15) are fixedly connected to the limiting part (1301) and the surface of the lower partition plate (105) respectively.

4. The device for collecting dental pulp stem cells according to claim 3, characterized in that: The cylindrical module (4) includes a hollow cylinder (401), a hollow rod (402) is fixedly installed at one end of the hollow cylinder (401) near the lower partition plate (105), the hollow rod (402) passes through the lower partition plate (105) and a knife holder (403) is fixedly installed thereon, and also includes a piston (404) installed inside the hollow cylinder (401), a push rod (405) is fixedly installed on the piston (404).

5. The device for collecting dental pulp stem cells according to claim 4, characterized in that: The tool holder (403) is provided with a guide groove (4031).

6. The device for collecting dental pulp stem cells according to claim 4, characterized in that: A liquid replenishment pipe (406) is fixedly installed on the hollow cylinder (401), and a one-way valve is fixedly installed inside both the liquid replenishment pipe (406) and the hollow rod (402).

7. The device for collecting dental pulp stem cells according to claim 4, characterized in that: A metal rod (16) is slidably installed inside the support base (1). A tension spring (17) is installed on the metal rod (16). A diagonal rod (18) is fixedly installed on one side of the metal rod (16). A diagonal groove (4051) adapted to the diagonal rod (18) is opened on the push rod (405). A coil (19) and a capacitor discharge power supply module (20) are installed inside the support base (1). The capacitor discharge power supply module (20) is electrically connected to the coil (19). Conductive contacts (21) are respectively provided on the nut seat (8) and the one-way gear (12). The conductive contacts (21) are connected in series in the discharge main circuit between the coil (19) and the capacitor discharge power supply module (20).

8. The device for collecting dental pulp stem cells according to claim 1, characterized in that: The collection bottle (3) includes a bottle body (301) and a bottle cap (302) connected by threads. The bottle cap (302) is provided with an outlet pipe (303) and an inlet pipe (304). A one-way valve is installed in both the outlet pipe (303) and the inlet pipe (304). The inlet pipe (304) is sealed to the flow pipe (201).

9. The device for collecting dental pulp stem cells according to claim 8, characterized in that: A filter membrane (305) is installed inside the bottle cap (302), and the end of the liquid outlet tube (303) located inside the bottle cap (302) passes through the filter membrane (305).

10. The device for collecting dental pulp stem cells according to claim 8, characterized in that: The bottle cap (302) has a positioning point (3021) fixedly installed on its outer edge surface, and the L-shaped support plate (101) has a positioning groove (1012).

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