Full-automatic closed gene sequencing library preparation instrument

Abstract

The invention discloses a full-automatic closed gene sequencing library preparation instrument, which relates to the technical field of gene sequencing and comprises a three-dimensional driving mechanism, an amplification module, an extraction module, a magnetic coupling stirring system and a waste liquid suction unit. The extraction module is provided with a plurality of reaction holes, a plurality of samples can be processed in parallel, and a driving mechanism is controlled by a program to realize accurate positioning and collaborative operation of functional components in a three-dimensional space. A magnetic stirring assembly and a cavity dividing structure are arranged in the special test tube. The instrument drives magnetic beads or a stirring rod in a test tube through an external rotating magnetic field to realize non-contact uniform mixing and nucleic acid extraction and purification, and in combination with an amplification module with precise temperature control, full-process library preparation steps such as splitting decomposition, reverse transcription, amplification, fragment separation and purification are automatically and sequentially completed in a closed system. According to the invention, high-flux, full-automatic and closed'sample inlet-product outlet 'operation is realized, the preparation efficiency and the result consistency and reliability are remarkably improved, and cross contamination is effectively avoided.

Description

Technical Field

[0001] This invention relates to the field of gene sequencing technology, and in particular to a fully automated closed gene sequencing library preparation instrument. Background Technology

[0002] In gene sequencing workflows, library preparation is a crucial preliminary step to ensure sequencing quality and efficiency. Traditional library preparation processes rely heavily on manual operations, resulting in cumbersome steps, time-consuming and labor-intensive processes, open operation that easily leads to sample contamination or cross-contamination, and poor experimental result stability. Existing automated library preparation equipment often suffers from insufficient sealing, limited multi-sample parallel processing capabilities, low precision in the coordination of various functional modules (such as extraction, amplification, purification, temperature control, and waste disposal), unstable extraction and purification efficiency, and insufficient temperature control accuracy, making it difficult to meet the practical application requirements of high-throughput, high-precision gene sequencing. Therefore, there is an urgent need for a fully automated library preparation device that can achieve closed operation, multi-sample parallel processing, and precise collaborative operation of various modules. Summary of the Invention

[0003] This invention provides a fully automated closed gene sequencing library preparation instrument, including a mounting base and an extraction component. Two first slide rails are longitudinally arranged on the top of the mounting base, and a first slider is slidably connected to each slide rail. A connecting frame is arranged above the first slide rails. The top of the first slider is fixedly connected to the bottom of the connecting frame. A first connecting plate is arranged between the first slide rails at the bottom of the connecting frame. A first motor is arranged between the first slide rails on the top of the mounting base. One output end of the first motor is fixedly connected to one end of a first lead screw. The first lead screw is threadedly connected to the middle of the first connecting plate. Two Peltiers are arranged on the top of the connecting frame. A base plate is arranged on top of the Peltiers. Limiting posts are arranged at the four corners of the top of the base plate, and limiting grooves are arranged on the top of the limiting posts. The extraction component is arranged above the limiting posts.

[0004] Preferably, the extraction component includes a mounting bracket, a second motor, a drive gear, a first driven gear, and a second driven gear; A mounting frame is provided above the limiting post, with the top of the limiting post penetrating the bottom plate of the mounting frame. A first driven gear is rotatably connected inside the mounting frame above the limiting post. A second motor is provided on one side of the connecting frame, with its top output end fixedly connected to a driving gear located inside the mounting frame. A second driven gear is also provided between adjacent first driven gears. The second motor drives the driving gear to rotate, which in turn drives the second driven gears to rotate synchronously with all the first driven gears. A first magnet is provided inside the lower side wall of the first driven gear. A reaction hole is provided on the top of the mounting frame corresponding to the position of the limiting post, and a test tube is placed inside the reaction hole.

[0005] Preferably, a support frame is provided on one side of the top connecting frame of the mounting base, and a second slide rail is provided on the side of the top sidewall of the support frame near the connecting frame. A second slider is slidably connected on the second slide rail. The side of the second slider near the connecting frame is fixedly connected to one side of the C-shaped plate. A third motor is provided at the bottom of the support frame. The top output end of the third motor is fixedly connected to one end of a second lead screw. The second lead screw passes through the C-shaped plate and is threadedly connected to the bottom of the C-shaped plate. The upper end of the side of the C-shaped plate near the connecting frame is fixedly connected to one side of the second connecting plate.

[0006] Preferably, the second connecting plate is fixedly connected to one end of the mounting plate on the side near the connecting frame. A fixing plate is provided at the bottom of the mounting plate near the second connecting plate. A third slide rail is provided on the side of the fixing plate away from the second connecting plate. A third slider is slidably connected on the third slide rail. The bottom of the third slider is fixedly connected to the top of the support frame. A fourth motor is provided on the side of the fixing plate away from the support frame. The output end of the fourth motor is fixedly connected to one end of the third lead screw. The third lead screw is threadedly connected to the side wall of the support frame.

[0007] Preferably, a first suspension plate is provided on one side of the support frame perpendicular to the mounting plate, a plurality of stirring motors are provided on the top of the first suspension plate, and the bottom output end of the stirring motor passes through the first suspension plate and is provided with a second magnet; A second suspension plate is installed perpendicularly to the mounting plate on the other side of the support frame. Multiple waste liquid needles are installed on the second suspension plate, and the second suspension plate is fixedly connected to the upper end of the waste liquid needles.

[0008] Preferably, the test tube is threaded with a sealing cap at the top, a suspension frame is provided at the top inside the test tube, a third magnet is provided inside the suspension frame, the bottom of the third magnet is fixedly connected to the top of the stirring rod, a sealing liquid injection mechanism is provided at the lower end inside the test tube, a reaction chamber is provided below the sealing liquid injection mechanism, and the inside of the test tube is connected to the reaction chamber.

[0009] Compared with the prior art, the beneficial effects of the present invention are: This invention achieves fully automated, closed-loop operation for gene sequencing library preparation, effectively solving the problems of cumbersome, time-consuming, and labor-intensive traditional manual operations, which are prone to sample contamination or cross-contamination and poor experimental result stability. It also overcomes the shortcomings of existing automated equipment, such as insufficient sealing, limited multi-sample parallel processing capability, low precision in the coordination of functional modules, unstable extraction and purification efficiency, and insufficient temperature control accuracy. Through multi-motor drive, the related components achieve precise three-dimensional movement in the vertical, horizontal, and vertical directions. Combined with eight reaction wells, it supports the parallel processing of up to eight samples, significantly improving high-throughput preparation efficiency. It integrates functions such as magnetic bead extraction, precise temperature control, liquid stirring and mixing, purification and cleaning, and automatic waste liquid aspiration. The Peltier motor provides stable heating, cooling, or temperature control to ensure reaction conditions, while the magnetically driven stirring motor ensures sufficient contact between the liquid in the test tube and the waste liquid syringe efficiently treats the waste liquid. The coordinated operation of all modules not only simplifies the library preparation process but also further improves the consistency and reliability of experimental results, better meeting the practical application needs of high-precision gene sequencing. Attached Figure Description

[0010] Figure 1 A three-dimensional structural schematic diagram of the present invention is shown; Figure 2 This invention is shown Figure 1 Enlarged cross-sectional view of the structure at point A in the image; Figure 3 This invention is shown Figure 1 Enlarged structural diagram at point B in the diagram; Figure 4 This invention is shown Figure 1 Enlarged bottom view of the structure at point C; Figure 5 An exploded view of the internal structure of the extraction component of this invention is shown. Figure 6 This invention is shown Figure 5 Enlarged cross-sectional view of the structure at point D in the image; Figure 7 A cross-sectional view of the internal structure of the test tube of the present invention is shown.

[0011] The components include: 1. Mounting base; 2. First slide rail; 201. First slider; 3. Connecting frame; 301. First connecting plate; 4. First motor; 5. First lead screw; 6. Peltier; 7. Base plate; 701. Limiting post; 7011. Limiting groove; 8. Mounting frame; 801. Reaction hole; 9. Second motor; 10. Driving gear; 11. First driven gear; 12. Second driven gear; 13. First magnet; 14. Support frame; 15. Second slide rail; 1501. Second slider; 16. C-shaped plate; 1 7. Third motor; 18. Second lead screw; 19. Second connecting plate; 20. Mounting plate; 2001. Fixing plate; 21. Third slide rail; 22. Third slider; 23. Bearing frame; 24. Fourth motor; 25. Third lead screw; 26. First suspension plate; 27. Stirring motor; 28. Second magnet; 29. ​​Second suspension plate; 2901. Waste liquid needle; 30. Test tube; 31. Sealing cap; 32. Suspension frame; 33. Third magnet; 34. Stirring rod; 35. Sealing injection mechanism; 36. Reaction chamber. Detailed Implementation

[0012] Various exemplary embodiments, features, and aspects of this disclosure will now be described in detail with reference to the accompanying drawings. The same reference numerals in the drawings denote elements that have the same or similar functions. Although various aspects of the embodiments are shown in the drawings, they are not necessarily drawn to scale unless specifically indicated otherwise.

[0013] The term “exemplary” as used herein means “serving as an example, embodiment, or illustration.” Any embodiment illustrated herein as “exemplary” is not necessarily to be construed as superior to or better than other embodiments.

[0014] Furthermore, to better illustrate this disclosure, numerous specific details are set forth in the following detailed description. Those skilled in the art will understand that this disclosure can be practiced without certain specific details. In some instances, methods, means, components, and circuits well known to those skilled in the art have not been described in detail in order to highlight the main points of this disclosure.

[0015] Please see Figure 1 — Figure 7 This invention provides an embodiment in which two first slide rails 2 are longitudinally arranged on the top of the mounting base 1, and a first slider 201 is slidably connected on the first slide rail 2. A connecting frame 3 is arranged above the first slide rail 2, and the top of the first slider 201 is fixedly connected to the bottom of the connecting frame 3. A first connecting plate 301 is arranged between the first slide rails 2 at the bottom of the connecting frame 3. A first motor 4 is arranged between the first slide rails 2 on the top of the mounting base 1, and one output end of the first motor 4 is fixedly connected to one end of a first lead screw 5. The first lead screw 5 is threadedly connected to the middle of the first connecting plate 301.

[0016] In the above embodiment, two first slide rails 2 are longitudinally arranged on the top of the mounting base 1, and a first slider 201 fixedly connected to the bottom of the connecting frame 3 is slidably connected to the first slide rail 2. At the same time, a first motor 4 is arranged on the mounting base 1, and its output end is threadedly connected to a first connecting plate 301 located at the bottom of the connecting frame 3 via a first lead screw 5. This arrangement constitutes a precise linear module. When the first motor 4 drives the first lead screw 5 to rotate, the first connecting plate 301 and the entire connecting frame 3 fixed thereto can be driven to move stably and accurately longitudinally along the first slide rails 2 through threaded transmission, thereby providing a reliable longitudinal feeding function for the components supported above.

[0017] In one embodiment of the present invention, two Peltiers 6 are arranged on the top of the connecting frame 3, and a base plate 7 is provided on the top of the Peltiers 6. Limiting posts 701 are provided at the four corners of the top of the base plate 7, and limiting grooves 7011 are provided on the top of the limiting posts 701.

[0018] In the above embodiments, two Peltiers 6 are arranged side by side on the top of the connecting frame 3 to provide precise temperature control; a base plate 7 is arranged above the Peltiers 6 as a heat-equalizing support platform; a limiting post 701 is provided at each of the four corners of the top of the base plate 7, and a limiting groove 7011 for positioning is opened at the top of each limiting post 701, which together constitute a stable and precise amplification module.

[0019] This invention provides an embodiment in which a mounting frame 8 is provided above a limiting post 701, with the top of the limiting post 701 penetrating the bottom plate of the mounting frame 8. First driven gears 11 are rotatably connected inside the mounting frame 8 above the limiting post 701. A second motor 9 is provided on one side of a connecting frame 3, with its top output end fixedly connected to a driving gear 10 located inside the mounting frame 8. A second driven gear 12 is also provided between adjacent first driven gears 11. The second motor 9 drives the driving gear 10 to rotate, thereby causing the second driven gear 12 to rotate synchronously with all the first driven gears 11. A first magnet 13 is provided inside the lower sidewall of the first driven gear 11. A reaction hole 801 is provided on the top of the mounting frame 8 corresponding to the position of the limiting post 701, and a test tube 30 is provided inside the reaction hole 801.

[0020] In the above embodiment, a mounting frame 8 is provided above the limiting post 701, and the limiting groove 7011 at the top of the limiting post 701 penetrates the bottom plate of the mounting frame 8 to achieve the positioning of the test tube 30. Several first driven gears 11 are rotatably connected inside the mounting frame 8, and adjacent first driven gears 11 are connected by meshing second driven gears 12. A second motor 9 is provided on one side of the connecting frame 3, and its output end is fixedly connected to the driving gear 10 located inside the mounting frame 8; the driving gear 10 meshes with the gear system to form a transmission chain. When the second motor 9 is started, it drives the driving gear 10 to rotate, which in turn drives all the first driven gears 11 to rotate synchronously through the second driven gears 12. A first magnet 13 is embedded in the lower side wall of each first driven gear 11. A reaction hole 801 is provided at the top of the mounting frame 8 corresponding to the position of the limiting post 701 for placing the test tube 30. This structure realizes the function of driving the movement of magnetic beads inside the test tube 30 by an external rotating magnetic field, thereby completing the closed nucleic acid extraction and mixing operation.

[0021] This invention provides an embodiment in which a support frame 14 is provided on one side of the top connecting frame 3 of the mounting base 1. A second slide rail 15 is provided on the top side wall of the support frame 14 near the connecting frame 3. A second slider 1501 is slidably connected to the second slide rail 15. The side of the second slider 1501 near the connecting frame 3 is fixedly connected to one side of the C-shaped plate 16. A third motor 17 is provided at the bottom of the support frame 14. The top output end of the third motor 17 is fixedly connected to one end of a second lead screw 18. The second lead screw 18 passes through the C-shaped plate 16 and is threadedly connected to the bottom of the C-shaped plate 16. The upper end of the side of the C-shaped plate 16 near the connecting frame 3 is fixedly connected to one side of the second connecting plate 19.

[0022] Through the above embodiments, a precision drive mechanism for vertical height adjustment is constructed. The support frame 14 serves as the main frame, and the second slide rail 15 and second slider 1501 mounted on it provide vertical guidance for the C-shaped plate 16. The third motor 17, located at the bottom of the support frame 14, drives the second lead screw 18 to rotate. Utilizing the threaded engagement between the lead screw and the bottom of the C-shaped plate 16, the rotational motion is converted into linear lifting and lowering motion of the C-shaped plate 16 and the second connecting plate 19 fixed above it along the second slide rail 15, thereby achieving precise control of the working height of the relevant functional components suspended on the second connecting plate 19.

[0023] In one embodiment of the present invention, the second connecting plate 19 is fixedly connected to one end of the mounting plate 20 on the side near the connecting frame 3. A fixing plate 2001 is provided at the bottom of the mounting plate 20 near the second connecting plate 19. A third slide rail 21 is provided on the side of the fixing plate 2001 away from the second connecting plate 19. A third slider 22 is slidably connected on the third slide rail 21. The bottom of the third slider 22 is fixedly connected to the top of the support frame 23. A fourth motor 24 is provided on the side of the fixing plate 2001 away from the support frame 23. The output end of the fourth motor 24 is fixedly connected to one end of the third lead screw 25. The third lead screw 25 is threadedly connected to the side wall of the support frame 23.

[0024] Through the above embodiments, a drive mechanism for precise horizontal positioning is constructed. The mounting plate 20, as a laterally extending base plate, has a third slide rail 21 and a third slider 22 at its bottom providing stable horizontal linear guidance for the support frame 23. A fourth motor 24, mounted on the fixed plate 2001, drives the third lead screw 25 to rotate. Utilizing the threaded engagement between the lead screw and the side wall of the support frame 23, the rotational motion is converted into horizontal linear reciprocating motion of the support frame 23 along the third slide rail 21, thereby achieving precise control and rapid switching of the horizontal working positions of the relevant functional modules mounted on the support frame 23.

[0025] In one embodiment of the present invention, a first suspension plate 26 is provided on one side of the support frame 23 perpendicular to the mounting plate 20. A plurality of stirring motors 27 are provided on the top of the first suspension plate 26, and the bottom output end of the stirring motor 27 passes through the first suspension plate 26 and is provided with a second magnet 28. Through the above embodiments, an independent stirring drive unit is constructed. The first suspension plate 26 serves as a mounting base, enabling multiple stirring motors 27 to be precisely aligned in space with the reaction hole 801. When the stirring motors 27 drive the second magnet 28 to rotate, a rotating magnetic field is generated inside the corresponding test tube 30 below, thereby driving the magnetic stirring components inside the test tube 30 to move, achieving efficient and gentle mixing of the reaction system, and ensuring the contact and mass transfer efficiency of the reactants.

[0026] In one embodiment of the present invention, a second suspension plate 29 is provided on the other side of the support frame 23 perpendicular to the mounting plate 20. A plurality of waste liquid needles 2901 are provided on the second suspension plate 29, and the upper end of the second suspension plate 29 is fixedly connected to the waste liquid needles 2901.

[0027] Through the above embodiments, an integrated waste liquid treatment unit is formed: the second suspension plate 29 serves as the mounting base, precisely positioning and fixing multiple waste liquid needles 2901 to the support frame 23, so that they can correspond one-to-one with the test tubes 30 in the reaction hole 801; under the control of the instrument, the support frame 23 can move the waste liquid needles 2901 to above each test tube 30, and automatically remove the waste liquid after the reaction is completed through the external negative pressure system, thereby achieving efficient, clean and automated cleaning of the reaction vessel.

[0028] This invention provides an embodiment in which a sealing cap 31 is threadedly connected to the top of the test tube 30, a suspension frame 32 is provided at the top inside the test tube 30, a third magnet 33 is provided inside the suspension frame 32, the bottom of the third magnet 33 is fixedly connected to the top of the stirring rod 34, a sealing liquid injection mechanism 35 is provided at the lower end inside the test tube 30, a reaction chamber 36 is provided below the sealing liquid injection mechanism 35, and the inside of the test tube 30 is connected to the reaction chamber 36.

[0029] Through the above embodiments, an integrated reaction vessel specifically designed for closed-loop automated operation has been constructed. The sealing cap 31 and the sealing injection mechanism 35 together ensure the airtightness of the test tube 30 throughout the entire process, effectively preventing contamination and evaporation. The internal magnetic stirring assembly, consisting of the third magnet 33 and the stirring rod 34, can achieve gentle and efficient non-contact mixing under the drive of the external rotating magnetic field, the second magnet 28. The reaction chamber 36, as the core reaction area, can sequentially complete a series of biochemical reactions such as cleavage, binding, washing, elution, and amplification.

[0030] Working principle: First, a series of special test tubes 30 pre-filled with different reagents are placed sequentially into the reaction holes 801 at the top of the mounting frame 8. The reaction chambers 36 at the bottom of the tubes are positioned by engaging the limiting grooves 7011 at the top of the limiting column 701. The Peltier 6 precisely controls the temperature rise and fall of the test tubes 30 through the base plate 7. When mixing or purification is required, the fourth motor 24 drives the third lead screw 25, causing the support frame 23 and its first suspension plate 26 to move laterally, positioning the stirring motor 27 and its bottom second magnet 28 above the test tubes 30. The stirring motor 27 drives the second magnet 28 to rotate, and its magnetic field penetrates the tube wall, driving the stirring assembly inside the test tube 30, which consists of the third magnet 33 and the stirring rod 34, to rotate, achieving non-contact mixing.

[0031] For the magnetic bead extraction operation, the second motor 9 drives the active gear 10, which in turn drives all the first driven gears 11 to rotate synchronously through the second driven gear 12. The first magnet 13 inside its sidewall generates a rotating magnetic field, which drives the magnetic beads in the test tube 30 to move, completing the capture, washing, and elution of nucleic acids. After the reaction is completed, the support frame 23 moves laterally so that the waste liquid needle 2901 on the second suspension plate 29 is aligned with the test tube 30, and the waste liquid is automatically removed by an external peristaltic pump. Throughout the process, the first motor 4 drives the connecting frame 3 to move longitudinally through the first lead screw 5, and the third motor 17 drives the C-shaped plate 16 and the mounting plate 20 to move vertically through the second lead screw 18. The three motors work together to achieve precise positioning and switching of the extraction module and the functional head in three-dimensional space, thereby automating and sequentially completing all library preparation steps such as lysis, reverse transcription, amplification, purification, and fragment sorting within the completely sealed test tube 30.

[0032] The various embodiments of this disclosure have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or technical improvements to the embodiments in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A fully automated closed-loop gene sequencing library preparation instrument, characterized in that: The assembly includes a mounting base (1) and an extraction component. The mounting base (1) has two first slide rails (2) arranged longitudinally on its top. A first slider (201) is slidably connected on the first slide rail (2). A connecting frame (3) is arranged above the first slide rail (2). The top of the first slider (201) is fixedly connected to the bottom of the connecting frame (3). A first connecting plate (301) is arranged between the first slide rails (2) at the bottom of the connecting frame (3). A first motor (4) is arranged between the first slide rails (2) on the top of the mounting base (1). One output end of the first motor (4) is fixedly connected to one end of the first lead screw (5). The first lead screw (5) is threadedly connected to the middle of the first connecting plate (301). Two Peltiers (6) are arranged on the top of the connecting frame (3). A base plate (7) is arranged on the top of the Peltiers (6). A limit post (701) is arranged at each of the four corners of the top of the base plate (7). A limit groove (7011) is arranged on the top of the limit post (701). An extraction component is arranged above the limit post (701).

2. The fully automated closed-loop gene sequencing library preparation instrument according to claim 1, characterized in that: The extraction assembly includes a mounting bracket (8), a second motor (9), a drive gear (10), a first driven gear (11), and a second driven gear (12). A mounting bracket (8) is provided above the limiting post (701). The top of the limiting post (701) passes through the bottom plate of the mounting bracket (8). The first driven gear (11) is rotatably connected inside the mounting bracket (8) above the limiting post (701). A second motor (9) is provided on one side of the connecting bracket (3). The top output end of the second motor (9) is fixedly connected to the driving gear (10) provided inside the mounting bracket (8). A second driven gear (12) is also provided between adjacent first driven gears (11). The second motor (9) drives the driving gear (10) to rotate, and then drives the second driven gear (12) to rotate synchronously with all the first driven gears (11). A first magnet (13) is provided in the lower side wall of the first driven gear (11). A reaction hole (801) is provided on the top of the mounting bracket (8) corresponding to the position of the limiting post (701). A test tube (30) is provided in the reaction hole (801).

3. The fully automated closed-loop gene sequencing library preparation instrument according to claim 2, characterized in that: The mounting base (1) has a support frame (14) on one side of the top connecting frame (3). The support frame (14) has a second slide rail (15) on the side of the top side wall near the connecting frame (3). A second slider (1501) is slidably connected on the second slide rail (15). The side of the second slider (1501) near the connecting frame (3) is fixedly connected to the side of the C-shaped plate (16). A third motor (17) is provided at the bottom of the support frame (14). The top output end of the third motor (17) is fixedly connected to one end of the second lead screw (18). The second lead screw (18) passes through the C-shaped plate (16) and is threaded to the bottom of the C-shaped plate (16). The upper end of the side of the C-shaped plate (16) near the connecting frame (3) is fixedly connected to the side of the second connecting plate (19).

4. The fully automated closed-loop gene sequencing library preparation instrument according to claim 3, characterized in that: The second connecting plate (19) is fixedly connected to one end of the mounting plate (20) on the side near the connecting frame (3). A fixing plate (2001) is provided at the bottom of the mounting plate (20) near the second connecting plate (19). A third slide rail (21) is provided on the side of the fixing plate (2001) at the bottom of the mounting plate (20) away from the second connecting plate (19). A third slider (22) is slidably connected on the third slide rail (21). The bottom of the third slider (22) is fixedly connected to the top of the support frame (23). A fourth motor (24) is provided on the side of the fixing plate (2001) away from the support frame (23). The output end of the fourth motor (24) is fixedly connected to one end of the third lead screw (25). The third lead screw (25) is threadedly connected to the side wall of the support frame (23).

5. The fully automated closed-loop gene sequencing library preparation instrument according to claim 4, characterized in that: A first suspension plate (26) is provided perpendicularly to the mounting plate (20) on one side of the support frame (23). Multiple stirring motors (27) are provided on the top of the first suspension plate (26). The bottom output end of the stirring motor (27) passes through the first suspension plate (26) and is provided with a second magnet (28). A second suspension plate (29) is provided perpendicularly to the mounting plate (20) on the other side of the support frame (23). Multiple waste liquid needles (2901) are provided on the second suspension plate (29). The second suspension plate (29) is fixedly connected to the upper end of the waste liquid needles (2901).

6. The fully automated closed-loop gene sequencing library preparation instrument according to claim 2, characterized in that: The test tube (30) is threaded with a sealing cap (31) at the top. A hanging frame (32) is provided at the top inside the test tube (30). A third magnet (33) is provided inside the hanging frame (32). The bottom of the third magnet (33) is fixedly connected to the top of the stirring rod (34). A sealing liquid injection mechanism (35) is provided at the lower end inside the test tube (30). A reaction chamber (36) is provided below the sealing liquid injection mechanism (35). The inside of the test tube (30) is connected to the reaction chamber (36).

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