Stem cell rotating and shaking device and use method thereof
By designing a stem cell rotation and mixing device with multi-dimensional motion and fixation mechanisms, the problems of single shaking action and insufficient fixation in existing technologies have been solved, achieving uniform mixing of culture medium and stability of the vessel, thereby improving the quality and efficiency of cell culture.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-13
- Publication Date
- 2026-03-17
AI Technical Summary
Existing stem cell mixing devices use a single shaking motion, which may result in insufficient fixation and cause the culture dish to loosen or fall off, affecting the uniform mixing of the culture medium and cell growth.
A stem cell rotation and mixing device was designed, which includes rotation, flipping and fixing mechanisms. It achieves uniform mixing of culture medium through multi-dimensional motion, prevents the vessel from loosening through fixing mechanisms, and ensures a stable environment by combining temperature and air filtration mechanisms.
This method achieves uniform mixing of the culture medium, improves the contact efficiency between cells and nutrients, ensures the stability of the vessel during rotation, provides a suitable growth environment, and improves the quality and efficiency of cell culture.
Smart Images
Figure CN121669052A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of stem cell mixing, and particularly relates to a stem cell rotating and mixing device and a use method thereof. BACKGROUND
[0002] Hematopoietic stem cells refer to stem cells in bone marrow, which have self-renewing ability and can differentiate into various blood cell precursor cells, and finally generate various blood cell components, including red blood cells, white blood cells and platelets, which can also differentiate into various other cells. Stem cells can save many people suffering from blood diseases, the most common of which is leukemia. After hematopoietic stem cells are extracted from the human body, they need to be mixed with protective agents in a low-temperature environment as soon as possible. At present, hematopoietic stem cells extracted from the human body are manually mixed and stored at low temperature by medical personnel.
[0003] During the culture of stem cells, the growth, proliferation and differentiation state of cells are influenced by multiple factors, among which the stability of the culture environment and the uniform distribution of nutrients are particularly crucial. The culture medium in the stem cell culture vessel needs to be mixed regularly to ensure that cells can fully contact with nutrient components, oxygen, and timely discharge metabolic waste, maintain the activity of cells and healthy growth state.
[0004] At present, the common stem cell mixing device has many deficiencies. On the one hand, the traditional mixing device can only realize a single shaking action, such as simple horizontal reciprocating shaking or vertical shaking. This single motion mode is difficult to fully simulate the complex stress environment of cells in vivo, resulting in uneven mixing of nutrients in the culture medium and uneven stress on cells, affecting the normal growth of cells. On the other hand, many devices are not firm and reliable when fixing the stem cell culture vessel, and the vessel is easy to loosen or even fall off during the mixing process, which not only pollutes the culture environment, but also may lead to cell culture failure, causing serious time and resource waste to researchers. SUMMARY
[0005] (I) Technical problems to be solved The purpose of the present application is to solve at least one of the above problems by providing a stem cell rotating and mixing device and a use method thereof, to solve the problem of single shaking action of the stem cell mixing device in the prior art and the possibility of loosening the culture vessel due to insufficient fixation, to achieve a multi-dimensional mixing function, to realize rotation and complex movements such as overturning, to ensure uniform mixing of the culture medium, and to avoid any loosening during the mixing process.
[0006] (II) Technical solutions The purpose of the present application is achieved by the following technical solutions: The application discloses a stem cell rotating and shaking device, which comprises a bottom plate, a rotating mechanism, a turnover mechanism, a fixing mechanism and a control mechanism. The rotating mechanism is arranged on the bottom plate. The turnover mechanism is arranged on the rotating mechanism. The fixing mechanism is arranged on the turnover mechanism and comprises a cover and a mounting frame. The control mechanism is electrically connected with the rotating mechanism, the turnover mechanism and the fixing mechanism.
[0007] The rotating mechanism drives the turnover mechanism and the fixing mechanism arranged thereon to rotate through its own rotating movement, so that the stem cells and nutrient substances in the culture vessel are fully mixed, and the shaking effect is improved.
[0008] The turnover mechanism can make the stem cell culture vessel fixed thereon turn over within a certain angle range, can change the flowing direction and state of the culture solution in the culture vessel, further enhances the mixing effect, makes the stem cells fully contact with the culture solution in different directions, and helps to prevent the cells from excessively gathering and depositing at the bottom of the culture vessel, and improves the culture quality of the cells.
[0009] The cover and the mounting frame of the fixing mechanism jointly form a closed space for accommodating and fixing the stem cell culture vessel, and prevent the culture vessel from shaking, shifting or toppling during the rotating and turning over.
[0010] The control mechanism is electrically connected with the rotating mechanism, the turnover mechanism and the fixing mechanism and other components, and centrally controls and coordinates them.
[0011] Further, the stem cell culture vessel comprises a test tube, a culture bottle and the like.
[0012] Further, the lower end of the cover is provided with a fixing motor, and the output end of the fixing motor is provided with a lead screw. The upper part of the fixing member is provided with a nut, the lead screw is connected with the nut in a matching mode, and when the fixing motor rotates, the fixing member is driven to move linearly up and down through the lead screw and the nut.
[0013] The fixing member exerts pressure on the stem cell culture vessel placed in the mounting groove, and firmly fixes the stem cell culture vessel on the mounting frame, so that the culture vessel is prevented from loosening or falling off due to vibration and the like during the shaking operation, and the reliability and safety of the shaking operation are ensured.
[0014] Further, the lead screw is a trapezoidal lead screw.
[0015] The lead screw is a trapezoidal lead screw, so when the nut rotates to press the stem cell culture vessel into the mounting rack, the lead screw is unlocked, and when the lead screw is locked, the nut cannot rotate by itself, and only when the motor drives the lead screw can the nut rotate, so after the fixing motor drives the fixing part to be pressed, it can maintain the pressed state of the fixing part without the fixing motor continuously outputting, preventing the fixing motor from being damaged by continuous operation.
[0016] Further, the lower part of the fixing part is provided with an inwardly recessed pressing groove, and the shape of the pressing groove matches the shape of the stem cell culture vessel; a pressure sensor is arranged in the pressing groove. The shape of the pressing groove matches the shape of the stem cell culture vessel, which can better fit the culture vessel and increase the fixing effect, and the pressure sensor in the pressing groove can monitor the pressure of the fixing part on the culture vessel in real time, avoiding damage to the culture vessel or adverse effects on stem cells due to excessive pressure.
[0017] Further, the bottom of the mounting rack is provided with a plurality of mounting grooves, and the bottom of the mounting groove is tapered. The plurality of mounting grooves arranged at the bottom of the mounting rack can fix a plurality of culture vessels at one time, improve the working efficiency of the device, meet the demand of large-scale stem cell culture, and the tapered design of the bottom of the mounting groove helps to better fix the culture vessel and increase the stability of the culture vessel during rotation and shaking.
[0018] Further, a temperature regulating assembly is arranged in the fixing mechanism, including a temperature sensor arranged at the bottom of the mounting groove and a heating element arranged at the outer side of the upper end of the mounting rack.
[0019] The temperature sensor is used to monitor the temperature change in the mounting groove in real time and feed back the temperature signal to the control mechanism, so that the control mechanism can accurately control the heating power of the heating element according to the preset temperature parameters, thereby realizing accurate regulation of the temperature of the stem cell culture environment and providing suitable temperature conditions for the growth and culture of stem cells, which is beneficial to the normal growth and metabolism of stem cells.
[0020] Further, the rotation shaft of the rotating mechanism is perpendicular to the bottom plate.
[0021] Further, the overturning mechanism includes a Y-shaped frame and an overturning motor. The bottom of the Y-shaped frame is fixedly connected with the rotating mechanism, the inner sides of the two ends of the Y-shaped frame are rotatably connected with the two sides of the fixing mechanism, and the overturning motor is arranged at the connection between the Y-shaped frame and the fixing mechanism.
[0022] Further, the shaking device further comprises an air filtering mechanism, including a gas pump and a one-way filter element, the gas pump is arranged on the side wall of the fixing mechanism, and the other side is connected with the one-way filter element. The one-way filter core only filters when the air pump draws air into the fixing mechanism.
[0023] Further, the pore size of the one-way filter core is 0.22 μm.
[0024] Further, the connection between the air pump and the one-way filter core is a quick-release connection, and the one-way filter core is replaceable.
[0025] Before the shaking work starts, the air pump can be used to exhaust air outward, so that a certain negative pressure is formed inside the fixing mechanism, which helps to exhaust the air inside and reduces the possibility of breeding of miscellaneous bacteria; when needed, the air pump is used to fill the filtered air into the fixing mechanism, so as to prevent miscellaneous bacteria from entering the inside of the fixing mechanism and adjust the internal air pressure balance, and avoid problems such as loosening of the culture vessel or poor sealing caused by air pressure change.
[0026] The second technical solution of the present application is the use method of the stem cell rotary shaking device, which comprises the following steps: 1. Open the upper cover, fix the stem cell culture vessel in the installation groove, drive the screw nut by the fixing motor, and make the fixing part move downward until the pressure value of the pressure sensor on the pressing groove reaches the standard of pressing, then stop the fixing motor, and lock the cover of the stem cell culture vessel by the fixing part; 2. Air pump, exhaust air outward until the fixing mechanism is in a vacuum sealed state, and then perform air extraction to ensure that no miscellaneous bacteria enter the fixing mechanism; 3. Start the rotating mechanism and the overturning mechanism, and mix.
[0027] (Three) beneficial effects Compared with the prior art, the present application has the following advantages: (1) The present application rotates the whole device by setting the rotating mechanism to drive the stem cell culture vessel to preliminarily mix the cells and nutrients in the stem cell culture vessel; at the same time, the overturning is increased on the basis of the rotation, the flow direction and state of the culture solution are changed, the mixing effect is further strengthened, the cells in the culture vessel can fully contact the nutrients and oxygen at each position, the utilization rate of the nutrients is improved, the growth and proliferation of the stem cells are promoted, and the growth state of the cells is effectively avoided to be affected by local nutrient deficiency or accumulation of metabolic waste.
[0028] (2) The fixing mechanism of the present invention is provided with multiple mounting brackets to provide stable mounting positions for multiple stem cell culture vessels. The fixing components firmly clamp the culture vessels through precise pressure control to ensure that the culture vessels will not loosen or fall off during rotation and flipping. The temperature control component can monitor and precisely control the temperature in the mounting tank in real time, so that the stem cells are always in a suitable temperature environment. The air filtration mechanism, through the cooperation of an air pump and a one-way filter, effectively filters the incoming air while ensuring stable internal air pressure, preventing the invasion of miscellaneous bacteria, and together create a stable, clean and suitable growth environment for stem cells, which is conducive to the long-term culture and experimental research of stem cells.
[0029] (3) The control mechanism of the present invention is electrically connected to each functional mechanism, realizing centralized control and automated operation of the entire shaking device. The device can automatically complete a series of complex operations such as fixing, shaking, and environmental regulation of stem cell culture vessels without frequent manual intervention, which greatly improves work efficiency and reduces the risk of operational errors. At the same time, it is also convenient to accurately control and record various parameters during the shaking process, providing reliable data support and quality assurance for scientific research on stem cell culture.
[0030] (4) The device of the present invention has a simple structure and is easy to implement. Attached Figure Description
[0031] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0032] Figure 1 This is a perspective view of a stem cell rotation and mixing device according to the present invention; Figure 2 This is a bottom view of a stem cell rotation and mixing device according to the present invention; Figure 3 This is a top view of a stem cell rotation and mixing device according to the present invention; Figure 4 This is a front view of a stem cell rotation and shaking device according to the present invention.
[0033] In the diagram: 1-Base plate; 2-Rotating mechanism; 3-Tilting mechanism; 31-Y-shaped frame; 32-Tilting motor; 4-Fixing mechanism; 41-Top cover; 42-Mounting bracket; 43-Fixing component; 44-Fixing motor; 45-Lead screw; 46-Nut; 47-Pressure groove; 48-Mounting groove; 49-Heating component; 5-Air filtration mechanism; 51-Air pump; 52-One-way filter element. Detailed Implementation
[0034] The application will be described in detail below with reference to the drawings and specific embodiments.
[0035] The following detailed description is presented in terms of specific embodiments illustrating the application. A person skilled in the art will recognize that the application can be practiced with modifications and changes within the scope and spirit of the application. Description of Embodiments
[0036] It is to be understood that the following description is merely descriptive in nature and is not intended to limit the scope of the application as described in the appended claims. It should be apparent that aspects described herein can be implemented in a wide variety of forms and that any specific structure and / or function described herein is merely illustrative. Based on the teachings herein one skilled in the art should appreciate that an aspect described herein can be implemented independently of any other aspects and that an aspect described herein can be implemented both as any number of software and / or hardware structures.
[0037] It is also noted that the illustrative figures can show various aspects of the application that can be implemented independently of one another or in various combinations, whether or not such combinations are shown in the figures. It is to be understood that the above description is intended to be illustrative, and not restrictive. Many other embodiments will be apparent to those of skill in the art upon reviewing the above description. The scope of the application should, therefore, be determined not with reference to the above description, but should instead be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled. The disclosures of all articles and patents are incorporated herein by reference in their entirety. Certain features, structures, or characteristics described herein can be implemented in software, hardware, or a combination thereof. The order in which some or all of the described steps are performed can be varied, and the order of steps can be changed.
[0038] Moreover, specific details are provided in the description to provide a thorough understanding of embodiments of the application. It will be apparent, however, to one of ordinary skill in the art that embodiments of the application can be practiced without some or all of these specific details.
[0039] The technical solutions provided by the embodiments of the application are described below with reference to the drawings. Embodiments
[0040] Reference Figures 1 to 4 The application provides a stem cell rotating and shaking device, which comprises a bottom plate 1, a rotating mechanism 2, a turnover mechanism 3, a fixing mechanism 4, an air filtering mechanism 5, and a control mechanism.
[0041] See Figure 1 The rotating mechanism 2 is mounted on the base plate 1, and the rotation axis of the rotating mechanism 2 is perpendicular to the base plate 1.
[0042] See Figure 1 The flipping mechanism 3 is mounted on the rotating mechanism 2 and includes a Y-shaped frame 31 and a flipping motor 32. The bottom of the Y-shaped frame 31 is fixedly connected to the rotating mechanism 2, and the inner sides of both ends of the Y-shaped frame 31 are rotatably connected to the two sides of the fixing mechanism 4. The flipping motor 32 is located at the connection between the Y-shaped frame 31 and the fixing mechanism 4.
[0043] See Figures 1 to 3 The fixing mechanism 4 is set on the flipping mechanism 3, including an upper cover 41 and a mounting bracket 42. The upper cover 41 is provided with a fixing member 43 for fixing the stem cell culture vessel set in the fixing mechanism 4. The lower end of the upper cover 41 is provided with a fixing motor 44, and the output end of the fixing motor 44 is provided with a lead screw 45. The upper part of the fixing member 43 is provided with a nut 46, and the lead screw 45 and the nut 46 are connected. When the fixing motor 44 rotates, it drives the fixing member 43 to move up and down linearly through the lead screw 45 and the nut 46. The lead screw 45 is a trapezoidal lead screw, and the fixing member 43 is a pressure plate.
[0044] See Figures 1 to 3 The lower part of the fixing member 43 is provided with an inwardly recessed groove 47. The shape of the groove 47 matches the shape of the stem cell culture vessel. A silicone sheet is attached to the groove 47 for anti-slip purposes. A pressure sensor is installed in the groove 47. The bottom of the mounting bracket 42 is provided with multiple mounting grooves 48. The bottom of the mounting groove 48 is conical.
[0045] See Figures 1 to 3 The fixing mechanism 4 is equipped with a temperature control component, including a temperature sensor located at the bottom of the mounting groove 48 and a heating element 49 located on the outer side of the upper end of the mounting bracket 42. The heating element 49 is a heating wire.
[0046] See Figure 3 The air filtration mechanism 5 includes an air pump 51 and a one-way filter element 52. The one-way filter element 52 has a pore size of 0.22 μm. The air pump 51 is located on the side wall of the fixed mechanism 4, and the other side is connected to the one-way filter element 52. The one-way filter element 52 filters only when the air pump 51 draws air into the fixed mechanism 4.
[0047] The control mechanism is electrically connected to the rotating mechanism 2, the flipping mechanism 3, the fixing mechanism 4, and the air filtering mechanism 5. Example
[0048] See Figures 1 to 4 The present invention provides a method for using a stem cell rotating and mixing device, comprising the following steps: 1. Open the top cover 41 and fix the stem cell culture vessel in the mounting groove 48. The fixed motor 44 drives the lead screw 45 to drive the nut 46, so that the fixing part 43 moves downward until the pressure value of the pressure sensor on the pressure groove 47 reaches the standard of tightening. The fixed motor 44 stops operating and the fixing part 43 locks the stem cell culture vessel cover. 2. Air pump 51 exhausts air until the fixing mechanism 4 is in a vacuum-sealed state, and then evacuates air to ensure that no bacteria enter the fixing mechanism 4. 3. Turn on the rotating mechanism 2 and the flipping mechanism 3 to mix them.
[0049] The same or similar parts between the various embodiments in this specification can be referred to mutually. Each embodiment focuses on describing the differences from other embodiments.
[0050] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
Claims
1. A stem cell rotation and shaking device comprising a base plate (1), characterized in that, The utility model relates to a rotating mechanism (2), a turnover mechanism (3), a fixing mechanism (4) and a control mechanism. The rotating mechanism (2) is arranged on the bottom plate (1). The turnover mechanism (3) is arranged on the rotating mechanism (2). The fixing mechanism (4) is arranged on the turnover mechanism (3) and comprises an upper cover (41) and a mounting frame (42). The control mechanism is electrically connected with the rotating mechanism (2), the turnover mechanism (3) and the fixing mechanism (4). The lower end of the upper cover (41) is provided with a fixing motor (44), and the output end of the fixing motor (44) is provided with a lead screw (45).
2. The stem cell spinner flask of claim 1, wherein, The upper part of the fixing part (43) is provided with a nut (46), the lead screw (45) is connected with the nut (46) in a matched mode, and when the fixing motor (44) rotates, the fixing part (43) is driven to move up and down in a straight line through the lead screw (45) and the nut (46). The lead screw (45) is a trapezoidal lead screw.
3. The stem cell spinner flask of claim 2, wherein, The lower part of the fixing part (43) is provided with an inwardly recessed pressing groove (47), the pressing groove (47) is matched with the shape of the stem cell culture vessel in shape, and a pressure sensor is arranged in the pressing groove (47).
4. The stem cell spinner flask of claim 2, wherein, The bottom of the mounting frame (42) is provided with a plurality of mounting grooves (48), and the bottom of the mounting groove (48) is tapered.
5. The stem cell spinner flask of claim 1, wherein, The fixing mechanism (4) is provided with a temperature regulating assembly, which comprises a temperature sensor arranged at the bottom of the mounting groove (48) and a heating element (49) arranged at the outer side of the upper end of the mounting frame (42).
6. The stem cell spinner flask of claim 5, wherein, The rotating shaft of the rotating mechanism (2) is perpendicular to the bottom plate (1).
7. The stem cell spinner flask of claim 1, wherein, The turnover mechanism (3) comprises a Y-shaped frame (31) and a turnover motor (32).
8. The stem cell spinner flask of claim 1, wherein, The bottom of the Y-shaped frame (31) is fixedly connected with the rotating mechanism (2), the inner sides of the two ends of the Y-shaped frame (31) are rotatably connected with the two sides of the fixing mechanism (4), and the turnover motor (32) is arranged at the connection position of the Y-shaped frame (31) and the fixing mechanism (4). The air filtering mechanism (5) comprises an air pump (51) and a one-way filter element (52), the air pump (51) is arranged on the side wall of the fixing mechanism (4), and the other side is connected with the one-way filter element (52).
9. The stem cell spinner flask of claim 1, wherein, The one-way filter element (52) only filters when the air pump (51) pumps air into the fixing mechanism (4). The utility model relates to a rotating mechanism (2), a turnover mechanism (3), a fixing mechanism (4) and a control mechanism.
10. The method of using a stem cell rotation device of any one of claims 1-9, wherein, (1) open the upper cover (41), fix the stem cell culture vessel in the mounting groove (48), drive the lead screw (45) by the fixing motor (44) to drive the nut (46), so that the fixing part (43) moves downward until the pressure value of the pressure sensor on the pressing groove (47) reaches the standard of pressing, the fixing motor (44) stops driving, and the fixing part (43) locks the stem cell culture vessel cover; (2) the air pump (51) pumps air outward until the fixing mechanism (4) is in a vacuum sealed state, and then air is pumped to ensure that no bacteria enter the fixing mechanism (4); (3) open the rotating mechanism (2) and the turnover mechanism (3) to mix.