A cell fluid dispensing device and dispensing method

Abstract

This invention provides a cell fluid dispensing device and method, aiming to solve the problem of cell fluid waste caused by tubing residue and calibration processes in existing technologies. By re-drawing the calibration solution after peristaltic pump flow calibration into the mixing bag, and incorporating steps such as air purging, tubing filling, and sensor calibration into the dispensing process, cell fluid residue can be effectively reduced and cell fluid utilization rate improved. This invention is suitable for the automated dispensing of high-value cell samples.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and specifically to a device and method for dispensing cell fluid. Background Technology

[0002] In the fields of biopharmaceuticals, cell therapy, and life science research, precise dispensing of cell solutions is a crucial step in experimental procedures and production processes. Highly active and valuable cell suspensions typically need to be precisely dispensed into multiple containers (such as cryovials, culture dishes, or reagent bottles) to ensure the consistency and effectiveness of subsequent experiments or treatments. Currently, automated or semi-automated cell solution dispensing systems are widely used, and their core components usually include a reservoir, fluid lines, a drive mechanism (such as a peristaltic pump), and a dispensing actuator.

[0003] In existing technologies, most mainstream cell fluid dispensing systems use disposable tubing and peristaltic pumps for cell fluid delivery. The typical operating procedure includes: attaching the disposable tubing to the peristaltic pump, where the pump's rotation and compression drive the directional movement of the cell fluid; before actual dispensing, system calibration is required, which involves rinsing the system and calibrating the flow rate using calibration solution or a sample of the cell fluid to be dispensed, to ensure dispensing accuracy. After calibration, the actual dispensing operation can then proceed.

[0004] However, existing methods have the following significant drawbacks, leading to a serious waste of valuable cell fluid resources:

[0005] 1. Significant Residue Inside Tubing Consumables: Due to the adhesive nature of cell fluid and the long, narrow inner diameter and complex path of disposable tubing (often containing multiple connectors, valves, or tees), a large amount of cell fluid remains on the inner wall and in dead spaces of the tubing after dispensing. This residual fluid cannot be effectively drained and utilized, and is directly discarded along with the consumables, resulting in the loss of high-value cell resources.

[0006] 2. The calibration process results in significant waste: During the peristaltic pump calibration phase, the system requires a certain volume of cell culture fluid (or calibration solution) to fill the tubing, remove air bubbles, and verify flow accuracy. This portion of cell culture fluid used for calibration is typically considered "contaminated" or "non-standard" after calibration and cannot be reused for formal dispensing; it must be discarded. For precious clinical or research-grade cell samples, this "one-time calibration" approach leads to unnecessary and avoidable waste.

[0007] 3. High overall cost: The aforementioned residues and calibration waste not only directly diminish the value of the cells themselves but also increase operating costs due to the frequent replacement of disposable consumables. Furthermore, existing solutions may employ more complex purging or rinsing processes to reduce residues, further increasing operational complexity and reagent consumption. Therefore, there is an urgent need for a novel liquid routing solution that can effectively reduce residues in tubing consumables during cell culture dispensing, optimize or avoid cell culture waste during calibration, significantly improve cell culture utilization while ensuring dispensing accuracy and sterility, and reduce experimental or production costs, especially suitable for processing high-value cell samples. Summary of the Invention

[0008] To address the aforementioned technical deficiencies, this invention provides a method and apparatus for dispensing cell fluid to avoid the severe waste of valuable cell fluid resources.

[0009] A method for dispensing cell fluid, characterized by comprising the following steps:

[0010] S1 obtains the mixing capacity of the mixing bag and the type of cells to be mixed;

[0011] S2 determines the number of cell sap bags, the corresponding cell types, and the capacity of the cell sap bags;

[0012] S3. Confirm the type of the mixed cell and the type of the dispensed cell to obtain the type confirmation result corresponding to each dispensed bag. Confirm the capacity of the mixed capacity and the capacity of each dispensed bag to obtain the capacity confirmation result corresponding to each dispensed bag.

[0013] S4 performs pre-emptive purging of the mixing bag to remove air from the bag; the cell solution in the cell solution bag is transferred to the mixing bag by the drive of the first peristaltic pump 401; the cells are mixed; the cell solution in the mixing bag is dispensed into cryopreservation bags by the drive of the second peristaltic pump 402.

[0014] The cell type to be aliquoted refers to the type of cells that need to be aliquoted into cryopreservation bags. Once the type of cells to be aliquoted is confirmed, the required mixing parameters can be determined.

[0015] Further, step S1 involves selecting the mixing bag capacity and cell fluid type via a PC software interface.

[0016] Furthermore, before performing the driving operation, the first peristaltic pump is calibrated and re-aspirated in the following manner: the first peristaltic pump is rotated in the forward direction to complete the calibration of the first peristaltic pump, and then the first peristaltic pump is rotated in the reverse direction to re-aspirate the cell fluid into the cell fluid bag.

[0017] Furthermore, the specific steps for dispensing the cell solution into the cryopreservation bag are as follows: connecting the cell solution bag and the mixing bag; the second peristaltic pump rotates in the forward direction to fill the cell solution into the mixing bag.

[0018] Furthermore, after completing the flow calibration of the second peristaltic pump and before filling the cryopreservation bag, the air in the cryopreservation bag is purged in the following manner: the second peristaltic pump is controlled to rotate in reverse to evacuate the gas in the cryopreservation bag. After the pressure in the cryopreservation bag is confirmed to have reached the threshold by the pressure sensor, the second peristaltic pump stops rotating in reverse.

[0019] Furthermore, after the second peristaltic pump is calibrated and before the cell solution is dispensed into the cryopreservation bags, close the clamp valves at the inlets of all cryopreservation bags and fill the tubing as follows: the second peristaltic pump rotates in the forward direction, allowing the cell solution to enter the dispensing tubing from the second peristaltic pump. Once the pressure in the dispensing tubing reaches the threshold, the second peristaltic pump stops rotating.

[0020] Furthermore, after the pipeline is filled, the filling process for each cryopreservation bag is started one by one.

[0021] Furthermore, after a cryopreservation bag is filled, the gas inside the cryopreservation bag is purged in the following manner: the second peristaltic pump is rotated in the reverse direction to extract air from the cryopreservation bag; the pressure value of the pressure sensor on the pipeline is detected to confirm that the gas inside the cryopreservation bag has been purged; the second peristaltic pump is rotated in the forward direction to transport the cell fluid in the pipeline between the pressure sensor and the cryopreservation bag back into the cryopreservation bag.

[0022] A cell fluid dispensing device includes the following structure: a first cell fluid bag connected to a first clamp valve, a second cell fluid bag connected to a second clamp valve, and a first filter connected to a fifth clamp valve. The first, second, and fifth clamp valves are all connected in parallel to one end of a first peristaltic pump. The other end of the first peristaltic pump is connected in parallel to two fluid paths: one path originates from the first peristaltic pump and is sequentially connected to a third clamp valve, a first calibration sensor J01, and a second filter; the other path originates from the first peristaltic pump and is sequentially connected to a fourth clamp valve, a first bubble detection device, a first pressure sensor, and one end of a mixing bag. The other end of the mixing bag is connected to one or more fluid paths in parallel. Multiple liquid filling circuits are provided. The first liquid filling circuit starts from the mixing bag and connects to the sixth clamp valve. The other end of the sixth clamp valve is connected in parallel to the seventh clamp valve and the second peristaltic pump. The other end of the seventh clamp valve is connected in sequence to the buffer pack and the third filter. The other end of the second peristaltic pump is connected in sequence to the eighth clamp valve, the second calibration sensor, the second pressure sensor, and one end of the second bubble detection device. Each of the multiple cryopreservation bags is connected to a cryopreservation bag clamp valve. The above-mentioned cryopreservation bag clamp valves are connected in parallel to the other end of the second bubble detection device. The one-way valve, after passing through the fourth filter, is also connected in parallel with the above-mentioned cryopreservation bag clamp valves to the other end of the second bubble detection device.

[0023] Furthermore, both the first calibration sensor and the second calibration sensor are ultrasonic flow sensors.

[0024] Furthermore, compared to traditional flow calibration bottles, the flow sensor is directly fixed to the pipeline, eliminating the need for a locally enlarged cavity in the traditional calibration bottle. This avoids the problem of liquid residue inside the calibration bottle and greatly reduces cell fluid waste. In addition, compared to traditional peristaltic pump calibration, it eliminates the need for inlet and outlet sensors at both ends of the calibration bottle, reducing costs and the complexity of software operation.

[0025] This invention uses the reverse rotation of a peristaltic pump after flow calibration to draw the calibration solution back into the mixing bag. It also incorporates steps such as air evacuation and tubing filling in the dispensing process, which effectively reduces cell fluid residue and improves cell fluid utilization. This invention is suitable for automated dispensing of high-value cell samples. Attached Figure Description

[0026] Figure 1 This is a flowchart of the process from cell sap bag to mixing bag provided in an embodiment of the present invention.

[0027] Figure 2 This is a flowchart of the operation from mixing bag to dispensing to cryopreservation bag provided by an embodiment of the present invention.

[0028] Figure 3 This is a schematic diagram of the liquid path structure of the cell fluid dispensing device according to an embodiment of the present invention.

[0029] Reference numerals: 101, First cell culture bag; 102, Second cell culture bag; 2, Mixing bag; 3, Cryopreservation bag; 401, First peristaltic pump; 402, Second peristaltic pump; 501, First pressure sensor; 502, Second pressure sensor; 6, One-way valve; H01, Buffer bag; F01, First clamp valve; F02, Second clamp valve; F03, Third clamp valve; F04, Fourth clamp valve; F05, Fifth clamp valve; F06, Sixth clamp valve; F07, Seventh clamp valve; F08, Eighth clamp valve; Fn, Cryopreservation bag clamp valve; L01, First filter; L02, Second filter; L03, Third filter; L04, Fourth filter; Q01, First bubble detection device; Q02, Second bubble detection device; J01, First calibration sensor; J02, Second calibration sensor. Detailed Implementation

[0030] This invention provides a cell fluid dispensing device, which includes: a first cell fluid bag 101, a second cell fluid bag 102, a mixing bag 2, a cryopreservation bag 3, a first peristaltic pump 401, a second peristaltic pump 402, a first pressure sensor 501, a second pressure sensor 502, a one-way valve 6, a buffer pack H01, a first clamp valve F01, a second clamp valve F02, a third clamp valve F03, a fifth clamp valve F05, a sixth clamp valve F06, a seventh clamp valve F07, an eighth clamp valve F08, a cryopreservation bag clamp valve Fn, a first filter L01, a second filter L02, a third filter L03, a fourth filter L04, a first bubble detection device Q01, a second bubble detection device Q02, a first calibration sensor J01, and a second calibration sensor J02. The above components are connected by consumable tubing, tee fittings, and other pipe joints to form a liquid circuit.

[0031] like Figure 3 As shown, the first cell fluid bag 101 is connected to the first clamp valve F01, the second cell fluid bag 102 is connected to the second clamp valve F02, and the first filter L01 is connected to the fifth clamp valve F05. The first clamp valve F01, the second clamp valve F02, and the fifth clamp valve F05 are connected in parallel to one end of the first peristaltic pump 401. The other end of the first peristaltic pump 401 is connected to two liquid paths in parallel: one path starts from the first peristaltic pump 401 and is connected in sequence to the third clamp valve F03, the first calibration sensor J01, and the second filter L02; the other path starts from the first peristaltic pump 401 and is connected in sequence to the fourth clamp valve F04, the first bubble detection device Q01, the first pressure sensor 501, and the mixing bag 2.

[0032] like Figure 3 As shown, the other end of the mixing bag 2 can be connected in parallel to multiple liquid filling lines, such as... Figure 3 The embodiment shown illustrates the case of two parallel liquid filling circuits. Among them: (1) The first liquid filling circuit starts from the mixing bag 2 and is connected to the sixth clamp valve F06. The other end of the sixth clamp valve F06 is connected in parallel to the seventh clamp valve F07 and the second peristaltic pump 402. The other end of the seventh clamp valve F07 is connected in sequence to the buffer pack H01 and the third filter L03. The other end of the second peristaltic pump 402 is connected in sequence to the eighth clamp valve F08, the second calibration sensor J02, the second pressure sensor 502, and the second bubble detection device Q02. Each of the eight cryopreservation bags 3 is connected in parallel to the other end of the second bubble detection device Q02 after passing through a cryopreservation bag clamp valve Fn. The one-way valve 6 is also connected in parallel to the other end of the second bubble detection device Q02 after passing through the fourth filter L04, so that the other end of the second bubble detection device Q02 forms a nine-way parallel structure. (2) After the second liquid filling channel starts from the mixing bag 2, the structure is the same as that of the first liquid filling channel, or the number and / or capacity of the cryopreservation bags in the second liquid filling channel are allowed to be different from those in the first liquid filling channel.

[0033] like Figure 3 As shown, the specific liquid path control of the cell solution dispensing device is as follows when the cell solution is transferred from the mixing bag to the cryopreservation bag.

[0034] (1) Pre-emptive venting: Connect the required consumable pipelines, select the mixing bag capacity, and click "Start" to close the first clamp valve F01, the second clamp valve F02, and the third clamp valve F03, and open the fourth clamp valve F04 and the fifth clamp valve F05. The first peristaltic pump 401 rotates in reverse to extract the gas from the mixing bag 2 and discharge it to the outside through the first filter L01. The first filter L01 ensures that the pipeline is in a sterile environment, preventing external contamination of the pipeline. Detect the value of the first pressure sensor 501. After reaching the corresponding threshold, the first peristaltic pump 401 stops rotating. Close the fourth clamp valve F04 and the fifth clamp valve F05.

[0035] (2) Calibration: After completing the above evacuation operation, open the first clamp valve F01 and the third clamp valve F03 to make the first peristaltic pump 401 rotate in the forward direction. The volume of liquid flowing through the sensor is obtained by detecting the first calibration sensor J01, thereby completing the flow calibration of the first peristaltic pump 401. After calibration, make the first peristaltic pump 401 rotate in the reverse direction to draw the cell fluid back into the first cell fluid bag 101 until the flow sensor detects that the amount of liquid drawn back is the same as the amount of liquid drawn forward, and the first peristaltic pump 401 stops moving.

[0036] (3) Infusion: After completing the above calibration, connect the first clamp valve F01 and the fourth clamp valve F04, and the first peristaltic pump 401 rotates in the forward direction to transfer the cell fluid in the first cell fluid bag 101 to the mixing bag 2. After the transfer in the first cell fluid bag 101 is completed, close the first clamp valve F01 and open the second clamp valve F02 to transfer the cell fluid in the second cell fluid bag 102. After the transfer in the second cell fluid bag 102 is completed, close all clamp valves.

[0037] (4) Mixing: Start cooling and mixing so that the cell solution in mixing bag 2 is mixed in a low temperature environment of 2-8℃.

[0038] This invention supports multiple parallel filling channels connected to the mixing bag for dispensing, and can simultaneously control the peristaltic pump on each filling channel for dispensing. The following is a detailed description of one of the filling channel solutions. Figure 3 As shown, the specific liquid path control of the cell solution dispensing device is as follows when the cell solution is transferred from the mixing bag to the cryopreservation bag.

[0039] (1) Pre-emptive venting: After cooling and mixing, start the filling of cryopreservation bags. First, close the sixth clamp valve F06, and at the same time open the seventh clamp valve F07 and the eighth clamp valve F08. Control the first of the multiple cryopreservation bag clamp valves Fn to open, while keeping the others closed. The second peristaltic pump 402 rotates in the opposite direction to vent the gas in the cryopreservation bag 3 connected to the first cryopreservation bag clamp valve. At the same time, detect the second pressure sensor 502. When the sensor reaches the threshold, close the first cryopreservation bag clamp valve Fn. Then, follow the above venting method to complete the pre-emptive venting action of the remaining cryopreservation bags 3 in sequence.

[0040] (2) Calibration: Open the sixth clamp valve F06 and the eighth clamp valve F08, and close the seventh clamp valve F07. The second peristaltic pump 402 rotates in the forward direction. The volume of liquid flowing through the sensor is obtained by detecting the second calibration sensor J02, thereby completing the flow calibration of the second peristaltic pump 402. After calibration, the second peristaltic pump 402 rotates in the reverse direction to draw the cell fluid back into the mixing bag 2 until the flow sensor detects that the amount of liquid drawn back is the same as the amount of liquid drawn forward. The second peristaltic pump 402 then stops moving, completing the flow calibration.

[0041] (3) Pipeline filling: After calibration, open the sixth clamp valve F06 and the eighth clamp valve F08, close the seventh clamp valve F07, and close all the clamp valves Fn of the cryopreservation bags; the second peristaltic pump 402 rotates in the forward direction to carry out pipeline filling work. During the filling process, the second pressure sensor 502 is detected. After the set threshold is reached, the second peristaltic pump 402 stops rotating, and the pipeline filling task is completed.

[0042] (4) Filling of cryopreservation bags: After the pipeline filling is completed, open the first cryopreservation bag clamp valve Fn, and the second peristaltic pump 402 rotates in the forward direction. When the first bubble detection device Q01 detects the cell fluid, it starts to measure the liquid volume and fills the cryopreservation bag connected to the first cryopreservation bag clamp valve Fn.

[0043] (5) Post-filling: After the cryopreservation bag connected to the first cryopreservation bag clamp valve Fn is filled, the second peristaltic pump 402 rotates in the reverse direction to perform the post-filling venting action of the filled cryopreservation bag. When the second bubble detection device Q02 detects bubbles and then detects liquid, the second peristaltic pump 402 rotates in the forward direction to transport the cell fluid in the pipeline into the cryopreservation bag, thus completing the filling of one cryopreservation bag. Other cryopreservation bags are filled in the same way as described above until all cryopreservation bags are filled.

[0044] The buffer pack H01 is in an open state during the exhaust and filling process, ensuring that trace amounts of liquid will not wet the third filter L03 when air is being introduced or removed, thus avoiding pipeline blockage.

[0045] The second bubble detection device Q02 should be installed as close as possible to the cryopreservation bag. This will reduce the impact of pipeline errors on the peristaltic pump's accuracy during the back-pushing process after the exhaust gas is released.

[0046] The peristaltic pump flow calibration sensor of this invention uses an ultrasonic flow sensor, which increases the flow rate and improves the calibration accuracy of the peristaltic pump. After the cell fluid in the final mixing bag 2 is emptied, the remaining cells can be pressed into the cryopreservation bag 3 by the forward rotation of the peristaltic pump with the help of air pressure, thus reducing the waste of cell fluid.

[0047] This invention provides a dispensing method using the above-described cell fluid dispensing device, the method comprising the following steps:

[0048] S1: Obtain the mixing capacity of the mixing bag and the type of cells to be mixed;

[0049] S2: Determine the number, type, and capacity of cell sap bags;

[0050] S3: Manually confirm the mixed cell type and each packaged cell type by selecting the corresponding option through the operation software interface to obtain the type confirmation result corresponding to each packaged bag, and confirm the capacity of each packaged bag to obtain the capacity confirmation result corresponding to each packaged bag.

[0051] S4: After receiving the dispensing order, according to the dispensing order, and the obtained cell types and capacities of each dispensing unit; according to... Figure 3 The liquid flow diagram describes the process of transferring the cell sap from the cell sap bag to the mixing bag for cell mixing, and then dispensing the mixture from the mixing bag into cryopreservation bags after mixing.

[0052] In step S1, when obtaining the mixing capacity and cell fluid type of the mixing bag, you can choose to select the mixing bag capacity and cell fluid type through the PC software interface.

[0053] In step S4, the mixing bag is first evacuated by controlling the clamp valve. After evacuation, the clamp valve connects the cell solution bag to the flow calibration sensor, and the peristaltic pump rotates forward to detect air bubbles and calibrate the flow rate. After calibration, the peristaltic pump rotates in reverse to reabsorb the cell solution. Then, the cryopreservation bag filling begins. After purging the gas from the cryopreservation bag, the flow pump is calibrated, and the clamp valve connects the cell solution bag to the mixing bag. The peristaltic pump rotates forward to fill the mixing bag with the cell solution. After filling, the clamp valve connects the filter to the mixing bag, and the peristaltic pump rotates in reverse to mix the air in the bag before purging.

[0054] Specifically, such as Figure 1As shown, the process of transferring cell fluid from the cell fluid bag to the mixing bag is as follows: (1) Pre-emptive purging: Control the clamp valve and rotate the first peristaltic pump 401 to purge the gas in the mixing bag 2. (2) Calibration: Control the clamp valve and extract the cell fluid from the cell fluid bag, which flows through the first calibration sensor J01 to perform flow rate calibration of the first peristaltic pump 401. After the flow rate calibration is completed, the peristaltic pump rotates in the reverse direction to draw back the cell fluid. (3) Infusion: Hang the cell fluid bags properly and control the clamp valve to extract the cell fluid from the cell fluid bags and infuse it into the mixing bag. (4) Mixing: Mix the cell fluid in the mixing bag in a low-temperature environment of 2-8℃.

[0055] like Figure 2 As shown, the process of cell fluid from the mixing bag to the cryopreservation bag is as follows: (1) Pre-emptive evacuation: Control the clamp valve, detect the pressure sensor, and extract the gas from the cryopreservation bag. (2) Calibration: Control the clamp valve, extract the cell fluid from the mixing bag, and flow through the second calibration sensor J02 to perform flow calibration of the second peristaltic pump 402. (3) Pipeline filling: Control the clamp valve, open the peristaltic pump, and detect the pressure sensor to perform pipeline filling. (4) Cryopreservation bag filling: Control the clamp valve, open the peristaltic pump to fill the cryopreservation bag. (5) Post-emptive evacuation: Control the clamp valve, open the peristaltic pump, and remove air bubbles from the cryopreservation bag.

[0056] like Figure 3 As shown, the flow calibration sensor in the process uses an ultrasonic flow sensor to ensure that the final cell solution is completely delivered to the cryopreservation bag while calibrating the flow rate, thus avoiding waste of the cell solution.

[0057] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for splitting a cell suspension, for transferring a cell suspension from a cell suspension bag to a pooling bag for pooling, and then to a plurality of cryobags, characterized in that, Includes the following steps: S1 obtains the mixing capacity of the mixing bag and the type of cells to be mixed; S2 determines the number of cell sap bags, the corresponding cell types, and the capacity of the cell sap bags; S3. Confirm the type of the mixed cell and the type of the dispensed cell to obtain the type confirmation result corresponding to each dispensed bag. Confirm the capacity of the mixed capacity and the capacity of each dispensed bag to obtain the capacity confirmation result corresponding to each dispensed bag. S4 performs a pre-emptive venting of the mixing bag to remove air from inside the bag; Before performing the driving operation, the first peristaltic pump (401) of S5 is calibrated and re-aspirated as follows: the first peristaltic pump (401) is rotated in the forward direction to complete the calibration of the first peristaltic pump (401), and then the first peristaltic pump (401) is rotated in the reverse direction to re-aspirate the cell fluid into the cell fluid bag; the cell fluid in the cell fluid bag is transferred to the mixing bag by the driving of the first peristaltic pump (401); S6 was used to mix the cells; S7 dispenses the cell solution from the mixing bag into cryopreservation bags via the drive of the second peristaltic pump (402); after a cryopreservation bag is filled, the gas inside the cryopreservation bag is purged in the following manner: the second peristaltic pump (402) is rotated in the reverse direction to extract air from the cryopreservation bag; the pressure value of the pressure sensor on the pipeline is detected to confirm that the gas inside the cryopreservation bag has been purged; the second peristaltic pump (402) is rotated in the forward direction to transport the cell solution in the pipeline between the pressure sensor and the cryopreservation bag back into the cryopreservation bag.

2. The method of claim 1, wherein: Step S1 involves selecting the mixing bag volume and cell culture type via the PC software interface.

3. The cell fluid dispensing method according to claim 1, characterized in that, After the flow rate calibration of the second peristaltic pump (402) is completed and before the cryopreservation bag is filled, the air in the cryopreservation bag is purged in the following manner: the second peristaltic pump (402) is controlled to rotate in reverse to evacuate the gas in the cryopreservation bag. After the pressure in the cryopreservation bag is confirmed to reach the threshold by the detection pressure sensor, the second peristaltic pump (402) stops the reverse rotation.

4. The cell fluid dispensing method according to claim 3, characterized in that, After the second peristaltic pump (402) is calibrated and before the cell solution is dispensed into the cryopreservation bags, close all clamp valves (Fn) at the inlet of the cryopreservation bags and fill the tubing as follows: the second peristaltic pump (402) rotates in the forward direction so that the cell solution enters the dispensing tubing from the second peristaltic pump (402). After the pressure in the dispensing tubing reaches the threshold, the second peristaltic pump (402) stops rotating.

5. The cell fluid dispensing method according to claim 4, characterized in that: After the pipeline is filled, the filling process for each cryopreservation bag is started one by one.

6. A cell fluid dispensing device, characterized in that, The structure includes the following: a first cell fluid bag (101) is connected to a first clamp valve (F01), a second cell fluid bag (102) is connected to a second clamp valve (F02), a first filter (L01) is connected to a fifth clamp valve (F05), and the first clamp valve (F01), the second clamp valve (F02) and the fifth clamp valve (F05) are all connected in parallel to one end of a first peristaltic pump (401). The other end of the first peristaltic pump (401) is connected in parallel to two liquid circuits: one circuit starts from the first peristaltic pump (401) and is connected in sequence to a third clamp valve (F03), a first calibration sensor (J01) and a second filter (L02); the other circuit starts from the first peristaltic pump (401) and is connected in sequence to a fourth clamp valve (F04), a first bubble detection device (Q01), a first pressure sensor (501) and one end of a mixing bag (2). The other end of the mixing bag (2) is connected to one or more canned liquid circuits. The first canned liquid circuit starts from the mixing bag (2) and is connected to the sixth clamp valve (F06). The other end of the sixth clamp valve (F06) is connected in parallel to the seventh clamp valve (F07) and the second peristaltic pump (402). The other end of the seventh clamp valve (F07) is connected in sequence to the buffer pack (H01) and the third filter (L03). The other end of the second peristaltic pump (402) is connected in sequence to the eighth clamp valve (F08), the second calibration sensor (J02), the second pressure sensor (502), and one end of the second bubble detection device (Q02). Each of the multiple cryopreservation bags (3) is connected to a cryopreservation bag clamp valve (Fn). The cryopreservation bag clamp valve is connected in parallel to the other end of the second bubble detection device (Q02). The one-way valve (6) passes through the fourth filter (L04) and is also connected in parallel with the cryopreservation bag clamp valve (Fn) to the other end of the second bubble detection device (Q02).

7. The cell fluid dispensing device according to claim 6, characterized in that: Both the first calibration sensor (J01) and the second calibration sensor (J02) are ultrasonic flow sensors.

8. The cell fluid dispensing device according to claim 7, characterized in that: The ultrasonic flow sensor calculates the flow velocity of the fluid by measuring the time difference, frequency difference, and / or phase difference of ultrasonic waves propagating in the fluid in the direction of flow and against the flow, thereby calculating the volumetric flow rate.

Images