Plasma separation and quantitative sampling integrated card shell and its use method

Abstract

The application relates to the technical field of plasma sampling, and discloses a plasma separation and quantitative sampling integrated cartridge and a use method thereof. The plasma separation and quantitative sampling integrated cartridge comprises an upper shell and a lower shell. A sample adding mechanism is arranged on the upper shell. The sample adding mechanism comprises a sample adding opening arranged on the top of the upper shell, a permeation opening arranged at the bottom of the sample adding opening, a sealing mechanism arranged on the top of the sample adding opening, and an inactivation mechanism arranged on the sealing mechanism. A flow guiding mechanism is arranged on the lower shell. The flow guiding mechanism comprises a mounting ring fixedly arranged on the top of the lower shell, and a lifting mechanism arranged at the bottom of the flow guiding mechanism. The application is characterized in that the sample adding separation structure and the flow guiding lifting mechanism are cooperatively designed, so that the plasma can be efficiently separated and guided out without relying on external equipment, the plasma can be blocked after sampling, the separation efficiency, sample purity and utilization rate are considered, the operation convenience and the structural stability of the device are improved, and the device is suitable for on-site rapid detection requirements.

Description

Technical Field

[0001] This invention belongs to the field of plasma sampling technology, specifically, it relates to an integrated plasma separation and quantitative sampling casing and its usage method. Background Technology

[0002] In clinical diagnosis, on-site rapid testing, and biosample analysis, efficient separation, accurate sampling, and standardized dilution of plasma samples are crucial preliminary steps to ensure reliable test results. Current traditional plasma processing procedures have many limitations and are ill-suited to the practical needs of efficient rapid testing. Most solutions rely on multiple independent devices such as centrifuges, pipettes, and dilution tubes, requiring separate steps: first, centrifuging to separate the plasma; then, manual pipetting; and finally, dilution in a dedicated container. This cumbersome process involves numerous steps, is time-consuming, and requires professional personnel. During manual sampling, the volume of liquid transferred is easily affected by the operator's technique, leading to insufficient quantitative accuracy and subsequent testing errors. Transferring samples between multiple devices increases the risk of exposure and contamination, compromising sample integrity. Furthermore, while some existing simplified processing devices attempt to streamline the process, they lack an integrated sampling and dilution design. After sampling, rapid and accurate transfer to the dilution stage is difficult, and poor compatibility between the sample and test strip during dilution further impacts testing efficiency and result stability, failing to meet the comprehensive requirements of convenience, accuracy, and efficiency for on-site rapid testing.

[0003] In view of this, the present invention is proposed. Summary of the Invention

[0004] To address the aforementioned technical issues, this invention provides an integrated plasma separation and quantitative sampling cassette, optimizing the synergy between plasma separation and inactivation functions, improving sample processing quality. Through a sealed and pressurized design at the sample dispensing port, blood separation is efficiently accelerated, shortening pretreatment time. The inactivating agent is sealed and stored within the cap, and its controlled release design allows for precise application to blood cells within the dispensing port, preventing premature sample contamination and ensuring effective inactivation. This provides a pure and safe plasma sample for subsequent testing, reducing detection errors. The invention achieves a closed-loop system for quantitative sampling, transfer, and dilution, adapting to diverse testing needs. The metering chamber automatically dispenses plasma quantitatively via capillary action, eliminating the need for manual measurement and improving sampling accuracy. The transfer mechanism precisely transfers plasma to the dilution tank, simplifying subsequent testing steps in conjunction with test strip adsorption and dilution operations.

[0005] The technical solution adopted by this invention to solve its technical problem is:

[0006] An integrated plasma separation and quantitative sampling casing includes: an upper shell and a lower shell; the upper shell is provided with a sample dispensing mechanism, the sample dispensing mechanism includes a sample dispensing port installed at the top of the upper shell, and a separation membrane is placed inside the sample dispensing port. A permeation port is provided at the bottom of the sample dispensing port, a sealing mechanism is provided at the top of the sample dispensing port, and an inactivation mechanism is provided on the sealing mechanism for inactivating the blood inside the sample dispensing port; the lower shell is provided with a flow guiding mechanism, the flow guiding mechanism includes a mounting ring fixedly installed at the top of the lower shell, a flow guiding element is movably connected inside the mounting ring, and a lifting mechanism is provided at the bottom of the flow guiding mechanism for driving the flow guiding element to rise and sealing the permeation port.

[0007] In a preferred embodiment of the present invention, the sealing mechanism includes a ring that is movably fitted onto the sample inlet, a cap that is fixedly installed on one side of the ring, the inactivation mechanism being placed inside the cap, and the cap being fastened onto the ring to form a sealing structure with the top of the sample inlet.

[0008] In a preferred embodiment of the present invention, the inactivation mechanism includes a storage tube fixedly installed inside the cover, with an upper plug and a lower plug respectively movably and sealingly connected to both ends of the storage tube. The upper plug, the lower plug and the storage tube form a closed space, and the closed space is filled with an inactivating agent.

[0009] In a preferred embodiment of the present invention, a transfer mechanism is provided on the lower housing. The transfer mechanism includes a metering cavity, with connecting rods fixedly installed at both ends of the metering cavity. A slider is fixedly installed at one end of each connecting rod, and the slider is located on both sides of the lower housing. A support protrusion is fixedly installed on the top of the lower housing, and the support protrusion supports the connecting rod.

[0010] In a preferred embodiment of the present invention, the upper housing is provided with a dilution mechanism, the dilution mechanism including a dilution tank opened on the top of the upper housing, and a test strip is placed in the dilution tank. After the metering chamber in the transfer mechanism takes a sample, it moves to the dilution tank and dilutes it.

[0011] In a preferred embodiment of the present invention, the lifting mechanism includes a rotating shaft, on which a limiting tube is movably sleeved. The limiting tube is fixedly installed on the top of the lower housing. One end of the rotating shaft movably passes through the mounting ring and extends into the interior. A second connecting rod is fixedly installed on one end of the rotating shaft. When the second connecting rod rotates around the rotating shaft, it lifts the guide component.

[0012] In a preferred embodiment of the present invention, the lifting mechanism further includes a triggering component, which includes a sliding plate. One end of the sliding plate has a guide surface, and a first connecting rod is fixedly installed on one side of the rotating shaft. When the sliding plate moves, it lifts the first connecting rod through the guide surface. Limiting protrusions are provided at both ends of the sliding plate. The limiting protrusions are fixedly installed on the top of the lower housing. A first connecting plate is fixedly installed on the sliding plate, and a second connecting plate is fixedly installed on one end of the first connecting plate. A groove is provided on the top of the sliding plate, and the second connecting plate is movably connected in the groove.

[0013] A method for using an integrated plasma separation and quantitative sampling cassette, based on the integrated plasma separation and quantitative sampling cassette, includes the following steps: S1, In use, blood is collected and added to the sampling port. After the cap is closed, the top of the sampling port is sealed. As the cap is closed, the internal pressure of the sampling port increases, promoting blood separation; S2, The separated plasma enters the mounting ring through the permeation port and enters the metering chamber under the action of the guide. Due to the adsorption force of the plasma on the surface of the metering chamber, the plasma smoothly fills the entire metering chamber.

[0014] As a preferred embodiment of the present invention, the following steps are also included: S3, sliding the slider, the slider drives the metering chamber to move through the connecting rod, transferring the plasma to the dilution tank, the dilution tank contains test strips, which absorb the plasma after contacting the metering chamber, and then diluent is added to the dilution tank for dilution; S4, when the slider is pushed, one end of the slide plate is blocked, the connecting rod moves and presses down the first connecting plate, so that the second connecting plate moves in the groove, and when the connecting rod passes the groove, the first connecting plate is reset.

[0015] As a preferred embodiment of the present invention, the following steps are also included: S5. During the dilution process, the slider is reset. At this time, the connecting rod abuts against the second connecting plate to make the slide plate move. The slide plate drives the first connecting rod to rotate through the guide surface, which in turn drives the rotating shaft and the second connecting rod to rotate. The second connecting rod lifts the guide piece to rise and block the permeation port; S6. The upper stopper is pushed and the lower stopper is opened. The inactivating agent in the storage tube enters the sample inlet to inactivate the blood cells therein.

[0016] Compared with the prior art, the present invention has the following advantages:

[0017] This invention optimizes the synergy between plasma separation and inactivation functions, improving sample processing quality. Through a sealed and pressurized design at the sample dispensing port, it efficiently accelerates blood separation and shortens pretreatment time. The inactivating agent is sealed and stored in the cap, and the controlled release design allows it to act precisely on blood cells in the sample dispensing port. This avoids premature contamination of the sample by the inactivating agent and ensures the inactivation effect, providing a pure and safe plasma sample for subsequent testing and reducing detection errors.

[0018] This invention achieves a closed-loop function of quantitative sampling, transfer and dilution, adapting to diverse testing needs. The metering chamber automatically and quantitatively extracts plasma based on capillary action, eliminating the need for manual measurement and improving sampling accuracy. The transfer mechanism can accurately transfer plasma to the dilution tank, which, together with the test strip adsorption and dilution operation, simplifies subsequent testing steps. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of the integrated plasma separation and quantitative sampling casing with the cover in the open state according to the present invention;

[0020] Figure 2 This is a schematic diagram of the closed state structure of the integrated plasma separation and quantitative sampling casing of the present invention;

[0021] Figure 3 This is a schematic diagram of the internal structure of the storage tube of the present invention;

[0022] Figure 4 This is a schematic diagram of the internal structure of the upper shell of the present invention;

[0023] Figure 5 This is a schematic diagram of the metering cavity structure of the present invention;

[0024] Figure 6 This is a schematic diagram of the structure at the mounting ring of the present invention;

[0025] Figure 7 For the present invention Figure 6 Schematic diagram of the structure at point A in the middle;

[0026] Figure 8 This is a schematic diagram of the flow guide component of the present invention.

[0027] Figure label:

[0028] 101. Upper shell; 102. Lower shell; 103. Sample inlet; 104. Inlet; 105. Sleeve; 106. Cap; 107. Storage tube; 108. Upper stopper; 109. Lower stopper; 110. Dilution tank; 111. Mounting ring; 112. Flow guide; 113. Metering chamber; 114. Connecting rod; 115. Sliding block; 116. Support protrusion; 117. Slide plate; 118. Guide surface; 119. First connecting rod; 120. Rotating shaft; 121. Second connecting rod; 122. Limiting tube; 123. Limiting protrusion; 124. Groove; 125. First connecting plate; 126. Second connecting plate. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate the present invention.

[0030] like Figures 1 to 8 As shown, an integrated plasma separation and quantitative sampling cassette includes: an upper shell 101 and a lower shell 102; the upper shell 101 is provided with a sample feeding mechanism, which includes a sample feeding port 103 installed on the top of the upper shell 101, and a separation membrane is placed inside the sample feeding port 103. A permeation port 104 is opened at the bottom of the sample feeding port 103, and the bottom of the permeation port 104 is inclined. A sealing mechanism is provided at the top of the sample feeding port 103, and an inactivation mechanism is provided on the sealing mechanism for inactivating the blood in the sample feeding port 103; the lower shell 102 is provided with a flow guiding mechanism, which includes a mounting ring 111 fixedly installed on the top of the lower shell 102. A flow guiding component 112 is movably connected inside the mounting ring 111. A lifting mechanism is provided at the bottom of the flow guiding mechanism for driving the flow guiding component 112 to rise and block the permeation port 104.

[0031] like Figures 1 to 3 As shown, in a specific embodiment, the sealing mechanism includes a sleeve 105 that is movably fitted onto the sample inlet 103. A cover 106 is fixedly installed on one side of the sleeve 105. The inactivation mechanism is placed inside the cover 106, and the cover 106 fastens onto the sleeve 105 to form a sealing structure with the top of the sample inlet 103. In this configuration, the sleeve 105 and the cover 106 are not integrally formed with the upper housing 101, which facilitates mass production through injection molding.

[0032] like Figures 1 to 3 As shown, the inactivation mechanism further includes a storage tube 107 fixedly installed inside the cap 106. An upper stopper 108 and a lower stopper 109 are movably and sealingly connected to both ends of the storage tube 107, forming a closed space between the upper stopper 108, the lower stopper 109, and the storage tube 107, which is filled with an inactivating agent. In this configuration, the cross-sectional diameters of the upper stopper 108 and the lower stopper 109 are slightly larger than the inner diameter of the storage tube 107 to increase the friction between the upper stopper 108 and the lower stopper 109 and the storage tube 107, preventing the pressure inside the sample inlet 103 from increasing and lifting the upper stopper 108 and the lower stopper 109 when the cap 106 is closed.

[0033] like Figures 4 to 6 As shown, the lower housing 102 is further provided with a transfer mechanism, which includes a metering chamber 113. Connecting rods 114 are fixedly installed at both ends of the metering chamber 113, and sliders 115 are fixedly installed at one end of each connecting rod 114. The sliders 115 are located on both sides of the lower housing 102. A support protrusion 116 is fixedly installed on the top of the lower housing 102, supporting the connecting rods 114. In this configuration, the metering chamber 113 has a relatively small inner diameter so that when it comes into contact with plasma, the plasma enters the metering chamber 113 by capillary action and fills the chamber, thereby achieving the function of quantitative sampling and transfer.

[0034] like Figure 1 , Figure 2 , Figure 4 , Figure 5 As shown, the upper housing 101 is further provided with a dilution mechanism, which includes a dilution tank 110 formed at the top of the upper housing 101. A test strip is placed in the dilution tank 110. After sampling, the metering chamber 113 in the transfer mechanism moves the sample to the dilution tank 110 for dilution. In this configuration, after the test strip comes into contact with the metering chamber 113, the plasma is directly absorbed by the test strip. At this time, the plasma on the test strip can be diluted by adding diluent to the dilution tank 110.

[0035] like Figure 6 , Figure 8 As shown, the lifting mechanism further includes a rotating shaft 120, on which a limiting tube 122 is movably sleeved. The limiting tube 122 is fixedly installed on the top of the lower housing 102. One end of the rotating shaft 120 movably passes through the mounting ring 111 and extends into the interior. A second connecting rod 121 is fixedly installed on one end of the rotating shaft 120. When the second connecting rod 121 rotates around the rotating shaft 120, it lifts the guide member 112. In this configuration, the limiting tube 122 is used to limit the rotating shaft 120. When the rotating shaft 120 rotates, it drives the second connecting rod 121 to rotate. The end of the second connecting rod 121 presses against the guide member 112 to make it rise and block the infiltration port 104.

[0036] like Figure 6 , Figure 7 , Figure 8 As shown, the lifting mechanism further includes a triggering component, which includes a slide plate 117. One end of the slide plate 117 has a guide surface 118. A first connecting rod 119 is fixedly installed on one side of the rotating shaft 120. When the slide plate 117 moves, it pushes up the first connecting rod 119 through the guide surface 118. Limiting protrusions 123 are provided at both ends of the slide plate 117. The limiting protrusions 123 are fixedly installed on the top of the lower housing 102. A first connecting plate 125 is fixedly installed on the slide plate 117, and a second connecting plate 126 is fixedly installed at one end of the first connecting plate 125. A groove 124 is provided on the top of the slide plate 117, and the second connecting plate 126 is movably connected in the groove 124. In this configuration, the first connecting plate 125 is made of elastic material. When the connecting rod 114 moves at the top of the first connecting plate 125, the first connecting plate 125 is pressed down. When the connecting rod 114 disengages from the first connecting plate 125, the first connecting plate 125 resets under its own elastic force. The end of the limiting protrusion 123 away from the guide surface 118 presses against the slide plate 117, so that the slide plate 117 can only move in the direction of the guide surface 118.

[0037] The implementation principle of the integrated plasma separation and quantitative sampling cassette in this embodiment is as follows: During use, blood is collected and added to the sampling port 103. Simultaneously, the cassette is gently shaken to allow the blood to cover the separation membrane. After the cap 106 is closed, the cap 106 seals the top of the sampling port 103. As the cap 106 is fastened to the sampling port 103, the pressure inside the sampling port 103 increases, causing rapid blood separation. The resulting plasma enters the mounting ring 111 through the permeation port 104, where it is guided by the flow guide 112. The plasma enters the measuring chamber 113. Due to the attraction between the surface of the plasma and the surface of the measuring chamber 113, the plasma can smoothly fill the measuring chamber 113 until it is full of plasma. The sliding slider 115 moves the measuring chamber 113 through the connecting rod 114 to transfer the plasma to the dilution tank 110. A test strip is placed in the dilution tank 110. After contacting the measuring chamber 113, the test strip absorbs the plasma. The plasma can then be diluted by adding diluent to the dilution tank 110.

[0038] During the process of pushing the slider 115, one end of the slide plate 117 is blocked. At this time, the connecting rod 114 moves and presses down the first connecting plate 125 so that the second connecting plate 126 moves in the groove 124. When the connecting rod 114 passes the groove 124, the first connecting plate 125 is reset. During the dilution process, the slider 115 is reset. At this time, the connecting rod 114 presses against the second connecting plate 126 so that the slide plate 117 moves. The slide plate 117 drives the first connecting rod 119 to rotate through the guide surface 118. The first connecting rod 119 drives the rotating shaft 120 to rotate. The rotating shaft 120 drives the second connecting rod 121 to rotate. The second connecting rod 121 lifts the guide member 112 to rise so as to block the permeation port 104. At this time, the upper plug 108 is pushed inward with tweezers. During the pushing process, the lower plug 109 is pushed open, so that the inactivating agent filled in the storage tube 107 enters the sample inlet 103 to inactivate the blood cells in the sample inlet 103.

[0039] The integrated plasma separation and quantitative sampling cassette of this application can be used not only for plasma separation, but also for the separation of samples such as urine, feces and saliva, with a wide range of applications.

[0040] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. An integrated cassette for plasma separation and quantitative sampling, characterized in that, include: The upper shell (101) and the lower shell (102) are provided with a sample feeding mechanism, which includes a sample feeding port (103) installed on the top of the upper shell (101), and a separation membrane is placed inside the sample feeding port (103). An infiltration port (104) is opened at the bottom of the sample feeding port (103). A sealing mechanism is provided at the top of the sample feeding port (103), and an inactivation mechanism is provided on the sealing mechanism. The inactivation mechanism is used to inactivate the blood in the sample feeding port (103). The lower shell (102) is provided with a flow guiding mechanism, which includes an installation ring (111) fixedly installed on the top of the lower shell (102). A flow guiding component (112) is movably connected inside the installation ring (111). A lifting mechanism is provided at the bottom of the flow guiding mechanism. The lifting mechanism is used to drive the flow guiding component (112) to rise and block the infiltration port (104). The sealing mechanism includes a ring (105) that is movably fitted onto the sample inlet (103). A cover (106) is fixedly installed on one side of the ring (105). The inactivation mechanism is placed inside the cover (106). The cover (106) is fastened onto the ring (105) and forms a sealing structure with the top of the sample inlet (103). The lifting mechanism includes a rotating shaft (120). A limiting tube (122) is movably fitted onto the rotating shaft (120). The limiting tube (122) is fixedly installed on the top of the lower housing (102). One end of the rotating shaft (120) movably passes through the mounting ring (111) and extends into the interior. A second connecting rod (121) is fixedly installed on one end of the rotating shaft (120). When the second connecting rod (121) rotates around the rotating shaft (120), it lifts the guide (112).

2. The integrated plasma separation and quantitative sampling casing according to claim 1, characterized in that, The inactivation mechanism includes a storage tube (107) fixedly installed inside the cover (106). The storage tube (107) is movably and sealed at both ends with an upper plug (108) and a lower plug (109). The upper plug (108), the lower plug (109) and the storage tube (107) form a closed space, and the closed space is filled with an inactivating agent.

3. The integrated plasma separation and quantitative sampling casing according to claim 2, characterized in that, The lower housing (102) is provided with a transfer mechanism, which includes a metering cavity (113). Connecting rods (114) are fixedly installed at both ends of the metering cavity (113). A slider (115) is fixedly installed at one end of the connecting rod (114). The slider (115) is located on both sides of the lower housing (102). A support protrusion (116) is fixedly installed on the top of the lower housing (102). The support protrusion (116) supports the connecting rod (114).

4. The integrated plasma separation and quantitative sampling casing according to claim 3, characterized in that, The upper housing (101) is provided with a dilution mechanism, which includes a dilution tank (110) opened on the top of the upper housing (101) and a test strip is placed in the dilution tank (110). The metering chamber (113) in the transfer mechanism takes a sample and moves it to the dilution tank (110) for dilution.

5. The integrated plasma separation and quantitative sampling casing according to claim 4, characterized in that, The lifting mechanism also includes a triggering component, which includes a slide plate (117). One end of the slide plate (117) is provided with a guide surface (118). A first connecting rod (119) is fixedly installed on one side of the rotating shaft (120). When the slide plate (117) moves, it lifts the first connecting rod (119) through the guide surface (118). Limiting protrusions (123) are provided at both ends of the slide plate (117). The limiting protrusions (123) are fixedly installed on the top of the lower housing (102). A first connecting plate (125) is fixedly installed on the slide plate (117), and a second connecting plate (126) is fixedly installed at one end of the first connecting plate (125). A groove (124) is provided on the top of the slide plate (117), and the second connecting plate (126) is movably connected in the groove (124).

6. A method for using an integrated plasma separation and quantitative sampling cartridge, based on the integrated plasma separation and quantitative sampling cartridge of claim 5, characterized in that, The method of using the integrated plasma separation and quantitative sampling cassette includes the following steps: S1. When in use, blood is collected and added to the sampling port (103). After the cap (106) is closed, the top of the sampling port (103) is sealed. As the cap (106) is closed, the internal volume of the sampling port (103) decreases and the internal pressure of the sampling port (103) increases, which promotes blood separation; S2. The plasma formed after separation enters the mounting ring (111) through the permeation port (104) and enters the metering chamber (113) under the action of the guide (112). Due to the adsorption force of the plasma on the surface of the metering chamber (113), the plasma smoothly fills the entire metering chamber (113).

7. The method of using the integrated plasma separation and quantitative sampling cartridge according to claim 6, characterized in that, The following steps are also included: S3, slide the slider (115), the slider (115) drives the metering chamber (113) to move through the connecting rod (114) to transfer the plasma to the dilution tank (110), the dilution tank (110) contains test strips, which adsorb plasma after contacting the metering chamber (113), and then diluent is added to the dilution tank (110) for dilution; S4, when the slider (115) is pushed, one end of the slide plate (117) is blocked, the connecting rod (114) moves and presses down the first connecting plate (125), so that the second connecting plate (126) moves in the groove (124), and when the connecting rod (114) passes the groove (124), the first connecting plate (125) is reset.

8. The method of using the integrated plasma separation and quantitative sampling cartridge according to claim 7, characterized in that, The process also includes the following steps: S5. During the dilution process, the slider (115) is reset. At this time, the connecting rod (114) abuts against the second connecting plate (126) to move the slide plate (117). The slide plate (117) drives the first connecting rod (119) to rotate through the guide surface (118), which in turn drives the rotating shaft (120) and the second connecting rod (121) to rotate. The second connecting rod (121) lifts the guide (112) to rise, so that it blocks the permeation port (104); S6. The upper stopper (108) is pushed, and the lower stopper (109) is pushed open. The inactivating agent in the storage tube (107) enters the sample inlet (103) to inactivate the blood cells therein.

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