Tableware washing composition

Abstract

The present invention relates to a dishwashing composition for reducing off-odor on a surface in a kitchen. In particular, the present invention relates to a cleaning composition for hard surfaces in galleries such as utensils, tabletops, stoves and soft surfaces such as sponges, cleaning cloths and wipes, which are tools for cleaning surfaces in galleries. The invention ensures that a surface remains fresh and free of unpleasant odors for a long period of time after being cleaned with the composition. This is accomplished by comprising a combination of rhamnolipids and bacillus bacterial spores in a dishwashing composition containing a surfactant.

Description

Technical Field

[0001] This invention relates to a dishwashing composition for reducing odors on surfaces. In particular, this invention relates to a cleaning composition that ensures that hard surfaces in the kitchen (such as utensils, countertops, stoves) and soft surfaces (such as sponges, cleaning cloths, and dishcloths (tools for cleaning kitchen surfaces)) remain fresh and odor-free for an extended period after cleaning with the composition. Background Technology

[0002] In the kitchen, consumers expect hard surfaces to remain clean, including utensils made of steel, ceramic, glass, etc. Other surfaces, such as countertops, stovetops, and other surfaces in the kitchen, also need to be kept clean. These hard surfaces in the kitchen sometimes have unpleasant odors, even though they may have been cleaned well. This may be due to residues of starch, protein, and oil left on them, which may decompose over time in the presence of residual water or through moisture condensing from humid air. In the kitchen, tools with soft surfaces (such as cleaning cloths, sponges, and brushes / rags used for cleaning utensils) can also produce odors due to the decomposition of the aforementioned residues. According to the present invention, a "dishwashing composition" refers to a composition for cleaning one or more of the aforementioned surfaces.

[0003] Therefore, when cleaning most surfaces in the kitchen (whether hard or soft), sufficient cleanliness and hygiene are required, while ensuring that odors are reduced for a period of time after the cleaning steps, using less harsh chemicals. In their search for a solution to this problem, the inventors of this invention surprisingly discovered Bacillus spp. (…) Bacillus The combination of bacterial spores and rhamnolipid biosurfactants not only cleans surfaces well but also ensures that the place smells fresh for a long time and produces minimal odor afterward.

[0004] Cleaning compositions containing biosurfactants are known, for example, US7556654B1, which discloses a combination of this type of surfactant with enzymes to produce cleaning benefits. This cleaning composition is shown to be formed through the action of bacteria and yeast on lipopeptides and sophorolipids, as well as enzymes derived from kelp. It does not disclose that a combination of rhamnolipids and Bacillus spores ensures a significant reduction in odors for an extended period after cleaning.

[0005] Therefore, one object of the present invention is to provide a cleaning composition for cleaning surfaces in the kitchen that provides long-term benefits against odors after cleaning. Summary of the Invention

[0006] A first aspect of the present invention relates to a dishwashing composition comprising: (i) 0.05 to 10% by weight of rhamnolipids; and (ii) 0.000005 to 1% by weight of Bacillus spores, which include Bacillus amyloliquefaciens (Bacillus) Bacillus amyloliquefaciens ), Bacillus megaterium ( Bacillus megaterium ) and Bacillus subtilis ( Bacillus subtilis ),and (iii) 1 to 30% by weight of anionic surfactants.

[0007] According to another aspect of the invention, a method for reducing odors on a surface is provided, the method comprising the step of contacting the surface with a composition of the invention, preferably diluted with water. Detailed Implementation

[0008] To avoid ambiguity, any feature of one aspect of the invention may be used in any other aspect of the invention. The word “comprising” is intended to mean “including”, but not necessarily “consisting of” or “made up of”. Thus, the term “comprising” means not limited to any of the elements subsequently stated, but optionally also covers unspecified elements of primary or secondary functional importance. In other words, the listed steps or options need not be exhaustive. Whenever the words “comprising” or “having” are used, these terms mean equivalent to “comprising” as defined above. It should be noted that the embodiments given in the following specification are intended to illustrate the invention and are not intended to limit the invention to those embodiments alone. Except as expressly stated in the embodiments or otherwise, all figures in this specification representing the amount of material or reaction conditions, the physical properties of the material, and / or uses should be understood to be modified by the word “about”. Unless otherwise stated, numerical ranges expressed in the format “x to y” should be understood to include both x and y. When multiple preferred ranges are described in the form of “x to y” for a particular feature, it should be understood that all ranges combining different endpoints are also considered. Unless otherwise stated, the amounts used herein are expressed as a weight percentage based on the total weight of the composition and are abbreviated as "weight%".

[0009] According to the present invention, "surfactant" refers to surfactants other than rhamnolipids described in the present invention. Therefore, "anionic surfactant" refers to anionic surfactants other than rhamnolipids described in the present invention.

[0010] The compositions of the present invention comprise rhamnolipids. Rhamnolipids are a class of glycolipids. They are composed of rhamnose in combination with β-hydroxy fatty acids. Rhamnose is a sugar. Fatty acids are ubiquitous in animals and plants.

[0011] Rhamnolipids are discussed in E. Deziel et al., Applied Microbiology and Biotechnology (2010) 86:1323-1336. Rhamnolipids are produced by Glycosurf, AGAE Technologies, and Urumqi Unite Bio-Technology Co., Ltd. Rhamnolipids can be produced by the bacteria *Pseudomonas aeruginosa* (…). Pseudomonas Aeruginosa Rhamnolipids are produced by strains of *Pseudomonas putida*. Pseudomonas Putida The recombinant cells produced contain at least one of the enzymes a / P hydrolase, rhamnosyltransferase I, or rhamnosyltransferase II with increased activity compared to wild-type cells.

[0012] Rhamnolipids are divided into two main groups: monorhamnolipids and dirhamnolipids. Monorhamnolipids have a single rhamnose ring. A typical monorhamnolipid produced by *Pseudomonas aeruginosa* is L-rhamnosyl-β-hydroxydecyl-β-hydroxydecanoate (RhaC). 10 C 10 It can be called Rha-C. 10 -C 10 , having formula C 26 H 48 O9. Monorhamnolipids have a single rhamnosine ring.

[0013] The IUPAC name is 3-[3-[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxane-2-yl]oxydecanoyloxy]decanoic acid.

[0014] Dirhamnolipids have two rhamnosine rings. A typical dirhamnolipid is L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (Rha2C). 10 C 10 It can be called Rha-Rha-C- 10 -C- 10 , having formula C 32 H 58 O 13 .

[0015] The IUPAC name is 3-[3-[4,5-dihydroxy-6-methyl-3-(3,4,5-trihydroxy-6-methyloxane-2-yl)oxyoxane-2-yl]oxydecanoyloxy]decanoic acid.

[0016] In practice, depending on the carbon source and bacterial strain, a variety of other minor components with different combinations of alkyl chain lengths exist alongside the more common rhamnolipide combinations described above. The ratio of monorhamnolipide to dirhamnolipide can be controlled by the production method. Some bacteria produce only monorhamnolipide (see US5767090: Example 1), and some enzymes can convert monorhamnolipide to dirhamnolipide.

[0017] In various published materials, monorhamnolipids are designated Rha-, which can be abbreviated as Rh or RL2. Similarly, dirhamnolipids are designated Rha-Rha or Rh-Rh or RL1. Due to historical reasons, "rhamnolipid 2" is a monorhamnolipid, and "rhamnolipid 1" is a dirhamnolipid. This has led to some ambiguity in the use of "RL1" and "RL2" in the literature.

[0018] Throughout this patent specification, we use the terms monorhamnolipid and dirhamnolipid to avoid such potential confusion. However, if the abbreviations are used, R1 is monorhamnolipid and R2 is dirhamnolipid. For more information on the confusion of terminology in the prior art, see the introduction to US 4814272.

[0019] The following rhamnolipids produced by the following bacteria have been detected: (C12:1, C14:1 represent fatty acyl chains with double bonds).

[0020] Rhamnolipids (monorhamnetin) produced by Pseudomonas aeruginosa: Rha-C8-C10, Rha-C10-C8, Rha-C10-C10, Rha-C10-C12, Rha-C10-C12:1, Rha-C12-C10, Rha-C12:1-C10 Rhamnolipids (di-rhamnolipids) produced by Pseudomonas aeruginosa: Rha-Rha-C8-C10, Rha-Rha-C8-C12:1, Rha-Rha-C10-C8, Rha-Rha-C10-C10, Rha-Rha-C10 -C12:1, Rha-Rha-C10-C12, Rha-Rha-C12-C10, Rha-Rha-C12:1-C-12, Rha-Rha-C10-C14:1 Rhamnolipids produced by Pseudomonas aeruginosa (not identified as monorhamnolipids or dirhamnolipids): C8-C8, C8-C10, C10-C8, C8-C12:1, C12:1-C8, C10-C10, C12-C10, C12:1-C10, C12-C12, C12:1-C12, C14-C10, C14:1-C10, C14-C14.

[0021] By Pseudomonas aeruginosa ( P. chlororaphis The rhamnolipin produced (monorhamnolipin only): Rha-C10-C8, Rha-C10-C10, Rha-C12-C10, Rha-C12:1-C10, Rha-C12-C12, Rha-C12:1-C12, Rha-C14-C10. Rha-C14:1-C10.

[0022] Burkholderia melioides (Berberis) Burkholdera pseudomallei The rhamnolipin produced (only dirhamnolipin): Rha-Rha-C14-C14.

[0023] Burkholderia plantarum ( Burkholdera plantarii (Pseudomonas plantarum) Pseudomonas plantarii The rhamnolipin produced (only dirhamnolipin): Rha-Rha-C14-C14.

[0024] The American Type Culture Collection (ATCC) holds over 100 strains of *Pseudomonas aeruginosa*. Many other strains are only available from manufacturers of commercial rhamnolipin. Furthermore, various research institutions worldwide may have isolated thousands of strains. Some work has progressed to typing them into groups. Each strain possesses distinct characteristics, including how much rhamnolipin it produces, what type of rhamnolipin it produces, what it metabolizes, and the conditions under which it grows. Only a small percentage of strains have been studied in depth.

[0025] Through evaluation and selection, strains of *Pseudomonas aeruginosa* can be isolated to produce rhamnolipids at higher concentrations and more efficiently. Strains can also be selected to produce fewer byproducts and metabolize different feedstocks or contaminants. This production is greatly influenced by the environment in which the bacteria grow.

[0026] A typical dirhamnolipid is L-rhamnosyl-L-rhamnosyl-β-hydroxydecyl-β-hydroxydecanoate (Rha2C) 10 C 10 , having formula C 32 H 58 O 13 ).

[0027] In practice, depending on the carbon source and bacterial strain, a variety of other minor components with different combinations of alkyl chain lengths exist alongside the more common rhamnolipide combinations described above. The ratio of monorhamnolipide to dirhamnolipide can be controlled by the production method. Some bacteria produce only monorhamnolipide (see US6767090: Example 1), and some enzymes can convert monorhamnolipide to dirhamnolipide.

[0028] Preferably, the rhamnose glycolipid is selected from: - Rhamnolipids (monorhamnetin) produced by Pseudomonas aeruginosa: Rha-C8-C10, Rha-C10-C8, Rha-C10-C10, Rha-C10-C12, Rha-C10-C12:1, Rha-C12-C10, Rha-C12:1-C10 - Rhamnolipids produced by *Pseudomonas aeruginosa* (monorhamnetin only): Rha-C10-C8, Rha-C10-C10, Rha-C12-C10, Rha-C12:1-C10, Rha-C12-C12, Rha-C12:1-C12, Rha-C14-C10, Rha-C14:1-C10.

[0029] - Monorhamnolipids can also be produced from *Pseudomonas aeruginosa* by introducing the genes rhlA and rhlB from *Pseudomonas aeruginosa* [Cha et al., Bioresour Technol. 2008. 99(7):2192-9] - Rhamnolipids (dirhamnolipids) produced by Pseudomonas aeruginosa: Rha-Rha-C8-C10, Rha-Rha-C8-C12:1, Rha-Rha-C10-C8, Rha-Rha-C10-C10, Rha-RhaC10 -C12:1, Rha-Rha-C10-C12, Rha-Rha-C12-C10, Rha-Rha-C12:1-C12, Rha-Rha-C10-C14:1 - Rhamnolipids produced by Burkholderia melioides (dirhamnolipids only): Rha-Rha-C14-C14.

[0030] - By Burkholderia plantarum ( Burkholdera plantarii (Pseudomonas plantarum) Pseudomonas plantarii The rhamnolipin produced (di-rhamnolipin only): Rha-Rha-C14-C14 - Rhamnolipids produced by Pseudomonas aeruginosa, which were not initially identified as monorhamnolipids or dirhamnolipids: C8-C8, C8-C10, C10-C8, C8-C12:1, C12:1-C8, C10-C10, C12-C10, C12:1-C10, C12-C12, C12:1-C12, C14-C10, C14:1-C10, C14-C14.

[0031] Preferably, the rhamnolipid is L-rhamnosyl-(3-hydroxydecanoyl-β-hydroxydecanoate) (having the formula C...26 H 48 O9's RhaC 10 C 10 ).

[0032] Preferably, the rhamnose glycolipid contains at least 50% by weight of dirhamnose glycolipid, more preferably at least 60% by weight of dirhamnose glycolipid, even more preferably 70% by weight of dirhamnose glycolipid, and most preferably at least 80% by weight of dirhamnose glycolipid.

[0033] Preferably, the rhamnose glycolipid is a dirhamnose glycolipid of the following formula: Rha2C 8-12 C 8-12 The preferred alkyl chain length is C8 to C9. 12 The alkyl chain can be saturated or unsaturated. The most preferred dirhamnolipid is an example of a dirhamnolipid with the following formula: Rha2C 8-12 C 8-12 This is referred to herein as rhamnolipin R2, and may be supplied by Evonik. The composition comprises 0.05 to 10%, preferably 0.1 to 5%, more preferably 0.5 to 3% by weight of the composition of rhamnolipin.

[0034] The compositions of the present invention comprise 0.000005 to 1% by weight of Bacillus spores comprising Bacillus amyloliquefaciens, Bacillus megaterium, and Bacillus subtilis. Preferred aspects involve compositions comprising Bacillus amyloliquefaciens, Bacillus megaterium, and Bacillus pumilus. Bacillus pumilus A composition comprising Bacillus subtilis spores. According to the present invention, the composition of bacterial spores means that bacterial spores may be included in the composition.

[0035] Another preferred aspect involves additionally including Bacillus licheniformis ( Bacillus licheniformis ) and Bacillus thuringiensis ( Bacillus thuringiensis Compositions of one or two of the following: Bacillus amyloliquefaciens, Bacillus megaterium, Bacillus subtilis, Bacillus pumilus, and Bacillus thuringiensis. A particularly preferred aspect of the invention relates to combinations comprising bacterial spores of Bacillus amyloliquefaciens, Bacillus megaterium, Bacillus subtilis, Bacillus pumilus, and Bacillus thuringiensis.

[0036] Preferably, each bacterial spore is present at 1 to 70% of the total number of bacterial spores, more preferably 5 to 40%. An even more preferred aspect of the invention relates to bacterial spores of *Bacillus amyloliquefaciens*, *Bacillus megaterium*, and *Bacillus subtilis* comprising 5% to 40% of the total number of bacterial spores. A particularly preferred aspect of the invention relates to bacterial spores of *Bacillus pumilus* and *Bacillus thuringiensis* each comprising 1 to 30%, preferably 20%, and most preferably 15% of the total number of bacterial spores.

[0037] Bacterial spores are contained in the composition at a weight percentage of 0.000005 to 1%, preferably 0.00005 to 0.75%, more preferably 0.0001 to 0.5%. The bacterial spores used in the compositions of the present invention can be counted to about 10. 11 The composition is in powder form at cfu / g, but other methods known to those skilled in the art for growing and introducing spores into the composition are also possible. Therefore, the bacterial spore content in the composition is preferably 10% of the composition. 3 Up to 10 11 cfu / g, more preferably 10 4 Up to 10 9 cfu / g, optimal value 10 5 Up to 10 8 cfu / g.

[0038] The compositions of this invention can be used to maintain kitchen items, such as utensils and tableware that can be cleaned using a dishwashing composition (preferably in liquid form). Various components other than the essential ingredients claimed in this invention are summarized below.

[0039] The compositions of the present invention comprise 1 to 30% by weight of an anionic surfactant. Preferred anionic surfactants are organic sulfates and sulfonates having an alkyl group containing about 8 to about 22 carbon atoms, the term "alkyl" used for alkyl moieties including higher acyl groups. Examples of such materials include alkyl sulfates, alkyl ether sulfates, alkylaryl sulfonates, α-olefin sulfonates, and mixtures thereof. The alkyl group preferably contains 10 to 18 carbon atoms and may be unsaturated. Alkyl ether sulfates may contain 1 to 10 ethylene oxide or propylene oxide units per molecule, preferably 1 to 3 ethylene oxide units per molecule. The counterions of the anionic surfactant are typically alkali metals, such as sodium or potassium; or ammonia counterions, such as monoethanolamine (MEA), diethanolamine (DEA), or triethanolamine (TEA). Mixtures of these counterions may also be used. Sodium and potassium are preferred.

[0040] The most preferred surfactants are alkylbenzene sulfonates, particularly straight-chain alkylbenzene sulfonates (LAS) with an alkyl chain length of 10 to 18 carbon atoms. Some alkyl sulfate surfactants (PAS), such as non-ethoxylated primary and secondary alkyl sulfates with an alkyl chain length of 10 to 18, can also be used.

[0041] Preferably, the composition further comprises an amphoteric surfactant. Preferably, the amphoteric surfactant is selected from alkylamine oxides, alkyl betaines, alkylamidopropyl betaines, alkyl sulfobetaine (sulfobetaine), alkyl glycinates, alkyl carboxyglycinates, alkyl amphoteric acetates, alkyl amphoteric propions, alkyl amphoteric glycinates, alkylamidopropyl hydroxysulfobetaine, acyl taurate, and acyl glutamate, having an alkyl group containing about 8 to about 22 carbon atoms, the term "alkyl" used for alkyl moieties including higher acyl groups. More preferably, the amphoteric surfactant is selected from alkylamidopropyl betaines, even more preferably cocamidopropyl betaines. When included, the amphoteric surfactant may be present in an amount preferably from 0.1 to 5% by weight, more preferably from 0.1 to 4% by weight, even more preferably from 1 to 3% by weight. Mixtures of any of the above materials may also be used. A favorable combination of surfactants of the present invention for use in liquid dishwashing compositions is a combination of 1 to 30% by weight of an anionic surfactant and 0.1 to 5% by weight of an amphoteric surfactant.

[0042] Preferably, the composition comprises a nonionic surfactant. Preferred types of nonionic surfactants used in this invention include aliphatic C8 to C96 surfactants. 18 C is preferred 12 To C 15 The linear primary alcohol ethoxylate has an average of 3 to 20, more preferably 5 to 10, moles of ethylene oxide per mole of alcohol. The preferred nonionic surfactant is a C16 / 18 alcohol ethoxylate. When included, the nonionic surfactant is present at 0.1 to 5% of the total weight of the composition.

[0043] The liquid dishwashing composition preferably contains an organic acid or a salt thereof. Preferably, the organic acid or a salt thereof may further contain citric acid, succinic acid, malic acid, lactic acid, tartaric acid, hexanoic acid, cyclohexanoic acid, heptanoic acid, octanoic acid, 4-methyloctanoic acid, nonanoic acid, decanoic acid, benzoic acid, 4-methoxybenzoic acid, and mixtures thereof. Examples of salts of organic acids include, preferably, salts of these organic acids formed with sodium and potassium, more preferably with sodium, such as trisodium citrate. More preferably, the organic acid or a salt thereof may further contain maleic acid or a salt thereof. Even more preferably, the organic acid or a salt thereof is selected from citric acid or a salt thereof, such as trisodium citrate. Preferably, the composition does not contain silver dihydrogen citrate.

[0044] The composition preferably contains 0.2 to 5% by weight, more preferably 0.3 to 4% by weight, even more preferably 0.4 to 3% by weight, and even more preferably 0.5 to 3% by weight, and most preferably 1 to 2% by weight of an organic acid or its salt.

[0045] Liquid dishwashing compositions may contain non-aqueous carriers, such as water-soluble solvents, co-solvents, and phase stabilizers. These materials are typically low molecular weight, water-soluble or water-miscible organic liquids, such as C1 to C5 monohydric alcohols (e.g., ethanol and n-propanol or isopropanol); C2 to C6 dihydric alcohols (e.g., propylene glycol and dipropylene glycol); C3 to C9 trihydric alcohols (e.g., glycerol); weight-average molecular weight (M... w The composition may include polyethylene glycol in the range of about 200 to 600; C1 to C3 alkanolamines, such as monoethanolamine, diethanolamine, and triethanolamine; and alkyl aryl sulfonates (such as xylene, toluene, ethylbenzene, and sodium and potassium cumene sulfonate) having up to 3 carbon atoms in the lower alkyl group. When included, a non-aqueous carrier may be present in an amount ranging from 0.1 to 3% by weight, preferably from 0.5 to 1% by weight, of the composition. Preferred co-water solvents are monopropylene glycol and glycerol.

[0046] Preferably, the liquid dishwashing composition further comprises water in an amount ranging from 5 to 99% by weight, more preferably from 10 to 90% by weight, even more preferably from 15 to 80% by weight, further preferably from 20 to 70% by weight, even more preferably from 30 to 65% by weight, even more preferably from 35 to 60% by weight, and even more preferably from 40 to 55% by weight.

[0047] Preferably, the liquid dishwashing composition further comprises one or more chelating agents. Preferably, the chelating agent may be present in an amount of 0.1 to 5% by weight, more preferably 0.25 to 4% by weight, or even more preferably 0.5 to 2.5% by weight.

[0048] Preferred chelating agents are phosphonic acids or their salts. Phosphonic acid (or its salt) chelating agents are preferably selected from 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP, commercially available as Dequest® 2010), diethylenetriaminepenta (DTPMP, commercially available as Dequest® 2066), hexamethylenediaminetetra(methylenephosphonic acid) (HDTMP), aminotri(methylenephosphonic acid) (ATMP), ethylenediaminetetra(methylenephosphonic acid) (EDTMP), tetramethylenediaminetetra(methylenephosphonic acid) (TDTMP); and phosphonobutanetricarboxylic acid (PBTC). The most preferred chelating agent is 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP). It is preferred that the chelating agent be added to the formulation in acid form.

[0049] Optionally, the liquid dishwashing composition further comprises an enzyme, with or without a suitable enzyme stabilizer. Preferably, the composition further comprises an effective amount of at least one enzyme, with or without a suitable enzyme stabilizer. Examples of suitable enzymes include pectinase, protease, amylase, cellulase, lipase, mannanase; with or without a suitable stabilizer. Optional and preferred aspects relate to dishwashing compositions that are substantially enzyme-free.

[0050] Preferably, the viscosity of the composition is at 25°C and 21 seconds. -1 The shear rate is in the range of about 200 mPa·s to about 10,000 mPa·s. This shear rate is the shear rate typically applied to the liquid when the bottle is poured. Pourable liquid home care compositions typically have a viscosity of 200 mPa·s to 1,500 mPa·s, preferably 100 mPa·s to 800 mPa·s.

[0051] The liquid dishwashing composition preferably has a pH of 2.0 to 8.0, more preferably 2.5 to 7.5, more preferably 3.0 to 7.0, even more preferably 3.5 to 7.0, further more preferably 4.0 to 7.5, still more preferably 4.5 to 7.5, still more preferably 5.0 to 7.5, still more preferably 5.5 to 7.5, and still more preferably 6.0 to 6.5.

[0052] The liquid dishwashing composition may further comprise one or more polymers. Preferably, the polymer can be cationic, anionic, amphoteric, or nonionic with a molecular weight greater than 100,000 Daltons. They are known to improve the viscosity and stability of the liquid composition, enhance the skin feel during and after use, and improve foam creaminess and foam stability. Examples of polymers include polyvinyl alcohol, polyacrylic acid, silanes, siloxanes, and mixtures thereof. If present, the polymer may preferably be present in the composition at an amount of 0.001 to 10% by weight, more preferably 0.1 to 6% by weight, and even more preferably 1 to 3% by weight.

[0053] Preferably, the composition can be used as is, i.e., clean, or it can be diluted before use. The degree of dilution usually depends on market preference. In some markets, a more concentrated product is required, while in other markets, a more diluted product is preferred. When the composition is a liquid dishwashing composition, it is typically diluted with water at a weight ratio ranging from 1:1 to 1:10.

[0054] Liquid dishwashing compositions may optionally contain ingredients such as fragrances, colorants, foam promoters, and odor-absorbing materials.

[0055] According to another aspect of the invention, a method for reducing odors on a surface is provided, the method comprising the step of contacting the surface with a composition of the invention, preferably diluted with water.

[0056] The invention will now be illustrated with the aid of the following non-limiting embodiments.

[0057] Example The rhamnolipids used in the following experiments were obtained from Evonik Industries, Germany, under the name ZZDL-RHEANCE.

[0058] Examples AF, 1-5: The effect of a combination of bacterial spores and rhamnolipin on kitchen odor reduction, as measured by olfactory testing. Function .

[0059] Take out the samples shown in Table 1 and measure the odor produced using the following method.

[0060] Kitchen odor testing : • Desized kitchen cloth - 5×5 cm used for experiments.

[0061] Add 1 ml of milk and rice starch water (milk + 5% starch 1:1) to the cloth. • Acinetobacter baumannii ( ) Acinetobacter baumanii ) and Oslomorula ( Moraxella osloensis (10) 8 CFU / ml attack • Add the formulation to the kitchen cloth. The control cloth was also kept without any formulation.

[0062] • Incubate the cups at 37°C for 48 hours. The odors are then scored by a trained panel.

[0063] Odor detection through olfactory testing Odor was measured on a scale of 0 (no kitchen odor) and 5 (strong kitchen odor). The untreated control had the strongest odor. Odor scores were given by a panel of five experts in a blinded test.

[0064] The bacterial spores in the table below are at a concentration of 10 5 Add 1 ml of cfu / ml.

[0065] Table 1:

[0066] In the table above, the following abbreviations are used for various bacterial spores: Ba: Bacillus amyloliquefaciens Bm: Bacillus megaterium Bs: Bacillus subtilis B1: Bacillus licheniformis Bp: Bacillus pumilus Bt: Bacillus thuringiensis @: refers to spores contained in the following amounts: 5 to 40% of the total number of bacterial spores contained, including Ba, Bm, and Bs, and 5 to 15% of Bp and Bt.

[0067] The data in the table above indicate that the combination of various bacterial spores of the Bacillus genus and rhamnolipids provides a synergistic interaction in reducing kitchen odors.

[0068] Examples GK and 6-9: Bacterial spores (at three different concentrations) and rhamnolipids measured by olfactory test The effect of combinations (at two different concentrations) on reducing kitchen odors .

[0069] Take out the samples shown in Table 2 and measure the generated odors using the kitchen odor measurement method as described in Examples AF and 1-5 above.

[0070] The bacterial spore combination (referred to as "probiotics") in the table below is Ba+Bm+Bs+Bp+Bt, with the following concentrations: Ba, Bm, and Bs account for 5-40% of the total number of bacterial spores, while Bp and Bt account for 5-15%.

[0071] Table 2

[0072] The data in the table above show that the present invention is effective in reducing kitchen odors for various concentrations of bacterial spores and rhamnolipid combinations.

Claims

1. A dishwashing composition comprising (i) 0.05 to 10 wt% rhamnolipid; and (ii) 0.000005 to 1 wt% spores of Bacillus bacteria comprising B. amyloliquefaciens, B. megaterium and B. subtilis, and (iii) 1 to 30 wt% anionic surfactant.

2. A composition according to claim 1 additionally comprising B. pumilus.

3. A composition according to claim 1 or 2 additionally comprising one or both of B. licheniformis and B. thuringiensis.

4. A composition according to any preceding claim additionally comprising 0.1 to 5 wt% amphoteric surfactant.

5. A method of reducing malodour on a surface comprising the step of contacting the surface with a composition according to any preceding claim, preferably diluted with water.

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