Multi-effect composition for resisting wrinkles, repairing, relieving and moisturizing and application of multi-effect composition

Abstract

The invention relates to the technical field of cosmetics. The invention provides a multi-effect composition for resisting wrinkles, repairing, relieving and moisturizing and application thereof. The composition is prepared from the following raw materials in parts by weight: 1 to 10 parts of agilawood extract and 0.1 to 2 parts of bisabolol. The product takes the agilawood extract as a main effective component, the preparation process is simple and convenient, and the use effect is excellent. The composition can be used for sensitive skin, has the effects of resisting wrinkles, repairing, relieving, moisturizing and the like, also has an anti-oxidation effect, and can effectively remove ABTS free radicals and delay skin aging.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a multi-effect composition for anti-wrinkle repair, soothing and moisturizing, and its application. Background Technology

[0002] The skin is the body's first line of defense, and its condition is a direct reflection of overall health. As people age, their ability to eliminate free radicals weakens, leading to skin aging.

[0003] Research by the Chinese Academy of Medical Sciences has discovered that agarwood extract possesses antioxidant and anti-inflammatory properties. Subsequently, agarwood extract was applied to cosmetics, confirming its beauty benefits for human skin, including wrinkle reduction, anti-inflammation, and anti-aging.

[0004] However, the existing types of agarwood skincare products are still very limited, and it is particularly important to expand their product range by developing them on a larger scale. Summary of the Invention

[0005] The purpose of this invention is to provide a multi-effect composition for anti-wrinkle repair, soothing and moisturizing, and its application. It can be used on sensitive skin and has anti-wrinkle, repairing, soothing and moisturizing effects. It can also effectively remove ABTS free radicals.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0007] This invention provides a multi-effect composition for anti-wrinkle repair, soothing and moisturizing, comprising the following raw materials in parts by weight: 1-10 parts of agarwood extract and 0.1-2 parts of bisabolol.

[0008] Preferably, the preparation method of the agarwood extract includes the following steps:

[0009] (1) Crush the raw agarwood into powder to obtain agarwood fragments;

[0010] (2) Distill the agarwood fragments to obtain the distillate;

[0011] (3) The distillate was separated into oil and water to obtain agarwood extract.

[0012] Preferably, the content of sesquiterpenoid compounds in the agarwood raw material in step (1) is ≥60%, the particle size of the agarwood fragments is >50 mesh, and the distillation in step (2) is carried out by steam distillation, the distillation temperature is 95-105℃, and the distillation time is 44-52h.

[0013] Preferably, the multi-effect composition further contains fractionated coconut oil, and the mass ratio of the agarwood extract, bisabolol and fractionated coconut oil is 1-10:0.1-2:88-98.9.

[0014] The present invention also provides a method for preparing the multi-effect composition, comprising the following steps:

[0015] (1) Weigh the raw materials according to the mass ratio of 1-10 parts of agarwood extract, 0.1-2 parts of bisabolol, and 88-98.9 parts of fractionated coconut oil;

[0016] (2) Agarwood extract, bisabolol and fractionated coconut oil are mixed and kept warm to obtain the multi-effect composition.

[0017] Preferably, the mixing temperature in step (2) is 28-32°C, the mixing time is 18-22 min, the rotation speed during the mixing process is 40-50 revolutions per minute, and the heat preservation time is 15-25 min.

[0018] The present invention also provides the use of the multi-effect composition in the preparation of anti-wrinkle, repairing, soothing and moisturizing products.

[0019] The present invention also provides an anti-wrinkle, repairing, soothing and moisturizing product, wherein the product comprises the multi-effect composition.

[0020] Preferably, the product also includes cosmetic-grade excipients, and the product types include face cream, lotion, face mask, eye cream, eye serum, neck cream, and body oil.

[0021] This invention provides a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing, and its application. The composition comprises the following raw materials in parts by weight: 1-10 parts of agarwood extract and 0.1-2 parts of bisabolol. The product of this invention uses agarwood extract as the main active ingredient, has a simple preparation process, and exhibits excellent effects. It can be used on sensitive skin, possessing anti-wrinkle, repairing, soothing, and moisturizing effects, as well as antioxidant effects, effectively scavenging ABTS free radicals and delaying skin aging. Detailed Implementation

[0022] This invention provides a multi-effect composition for anti-wrinkle repair, soothing and moisturizing, comprising the following raw materials in parts by weight: 1-10 parts of agarwood extract and 0.1-2 parts of bisabolol.

[0023] In this invention, the amount of agarwood extract is preferably 2 to 8 parts, and more preferably 5 to 6 parts.

[0024] In this invention, the amount of bisabolol is preferably 0.5 to 1.5 parts, and more preferably 1 part.

[0025] In this invention, the preparation method of the agarwood extract includes the following steps:

[0026] (1) Crush the raw agarwood into powder to obtain agarwood fragments;

[0027] (2) Distill the agarwood fragments to obtain the distillate;

[0028] (3) The distillate was separated into oil and water to obtain agarwood extract.

[0029] In this invention, the content of sesquiterpenoid compounds in the agarwood raw material in step (1) is ≥60%, the particle size of the agarwood fragments is >50 mesh, the distillation in step (2) is carried out by steam distillation, the distillation temperature is preferably 95-105℃, more preferably 100℃, and the distillation time is preferably 44-52h, more preferably 48h.

[0030] In this invention, the multi-effect composition further contains fractionated coconut oil, and the mass ratio of the agarwood extract, bisabolol and fractionated coconut oil is preferably 1-10:0.1-2:88-98.9, more preferably 5:1:94.

[0031] The present invention also provides a method for preparing the multi-effect composition, comprising the following steps:

[0032] (1) Weigh the raw materials according to the mass ratio of 1-10 parts of agarwood extract, 0.1-2 parts of bisabolol, and 88-98.9 parts of fractionated coconut oil;

[0033] (2) Agarwood extract, bisabolol and fractionated coconut oil are mixed and kept warm to obtain the multi-effect composition.

[0034] In this invention, the mixing temperature in step (2) is preferably 28-32°C, more preferably 30°C, the mixing time is preferably 18-22 min, more preferably 20 min, the rotation speed during the mixing process is preferably 40-50 revolutions per minute, more preferably 45 revolutions per minute, and the heat preservation time is preferably 15-25 min, more preferably 20 min.

[0035] The present invention also provides the use of the multi-effect composition in the preparation of anti-wrinkle, repairing, soothing and moisturizing products.

[0036] The present invention also provides an anti-wrinkle, repairing, soothing and moisturizing product, wherein the product comprises the multi-effect composition.

[0037] In this invention, the product also includes cosmetic-grade excipients, and the product is preferably a face cream, lotion, face mask, eye cream, eye serum, neck cream, or body oil.

[0038] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0039] Example 1

[0040] A method for preparing a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing includes the following steps:

[0041] (1) Select agarwood raw materials with a content of ≥60% of sesquiterpenoids, and crush the agarwood raw materials to 50 mesh or more to obtain agarwood fragments;

[0042] (2) Distill the agarwood fragments at 95℃ for 52 hours to obtain the distillate;

[0043] (3) The distillate was subjected to oil-water separation to obtain agarwood extract;

[0044] (4) Mix 1 part agarwood extract and 2 parts bisabolol by mass to obtain a multi-effect composition for anti-wrinkle repair, soothing and moisturizing.

[0045] Example 2

[0046] A method for preparing a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing includes the following steps:

[0047] (1) Select agarwood raw materials with a content of ≥60% of sesquiterpenoids, and crush the agarwood raw materials to 50 mesh or more to obtain agarwood fragments;

[0048] (2) Distill the agarwood fragments at 105℃ for 44 hours to obtain the distillate;

[0049] (3) The distillate was subjected to oil-water separation to obtain agarwood extract;

[0050] (4) Mix 10 parts of agarwood extract and 0.1 parts of bisabolol by mass to obtain a multi-effect composition for anti-wrinkle repair, soothing and moisturizing.

[0051] Example 3

[0052] A method for preparing a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing includes the following steps:

[0053] (1) Select agarwood raw materials with a content of ≥60% of sesquiterpenoids, and crush the agarwood raw materials to 50 mesh or more to obtain agarwood fragments;

[0054] (2) Distill the agarwood fragments at 100℃ for 48 hours to obtain the distillate;

[0055] (3) The distillate was subjected to oil-water separation to obtain agarwood extract;

[0056] (4) Mix 5 parts of agarwood extract and 1 part of bisabolol by mass to obtain a multi-effect composition for anti-wrinkle repair, soothing and moisturizing.

[0057] Example 4

[0058] A method for preparing a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing includes the following steps:

[0059] (1) Select agarwood raw materials with a content of ≥60% of sesquiterpenoids, and crush the agarwood raw materials to 50 mesh or more to obtain agarwood fragments;

[0060] (2) Distill the agarwood fragments at 100℃ for 48 hours to obtain the distillate;

[0061] (3) The distillate was subjected to oil-water separation to obtain agarwood extract;

[0062] (4) Weigh the raw materials according to the mass ratio of 10 parts agarwood extract, 2 parts bisabolol and 88 parts fractionated coconut oil;

[0063] (5) Mix agarwood extract, bisabolol and fractionated coconut oil at 32°C, stir at 40 revolutions per minute for 22 minutes, and keep warm for 15 minutes to obtain the anti-wrinkle, repairing, soothing and moisturizing multi-effect composition.

[0064] Example 5

[0065] A method for preparing a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing includes the following steps:

[0066] (1) Select agarwood raw materials with a content of ≥60% of sesquiterpenoids, and crush the agarwood raw materials to 50 mesh or more to obtain agarwood fragments;

[0067] (2) Distill the agarwood fragments at 100℃ for 48 hours to obtain the distillate;

[0068] (3) The distillate was subjected to oil-water separation to obtain agarwood extract;

[0069] (4) Weigh the raw materials according to the mass ratio of 1 part agarwood extract, 0.1 part bisabolol and 98.9 parts fractionated coconut oil;

[0070] (5) Agarwood extract, bisabolol and fractionated coconut oil are mixed at 28°C and stirred at 50 revolutions per minute for 18 minutes. After keeping warm for 25 minutes, the anti-wrinkle, repairing, soothing and moisturizing multi-effect composition is obtained.

[0071] Example 6

[0072] A method for preparing a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing includes the following steps:

[0073] (1) Select agarwood raw materials with a content of ≥60% of sesquiterpenoids, and crush the agarwood raw materials to 50 mesh or more to obtain agarwood fragments;

[0074] (2) Distill the agarwood fragments at 100℃ for 48 hours to obtain the distillate;

[0075] (3) The distillate was subjected to oil-water separation to obtain agarwood extract;

[0076] (4) Weigh the raw materials according to the mass ratio of 5 parts agarwood extract, 1 part bisabolol and 94 parts fractionated coconut oil;

[0077] (5) Mix agarwood extract, bisabolol and fractionated coconut oil at 30°C, stir at 45 revolutions per minute for 20 minutes, and keep warm for 20 minutes to obtain the anti-wrinkle, repairing, soothing and moisturizing multi-effect composition.

[0078] Experimental Example 1: Bacterial Detection

[0079] The anti-wrinkle, repairing, soothing and moisturizing multi-effect composition prepared in Example 6 was sent to Huace Testing and Certification Group Co., Ltd. for testing of total bacterial count, total mold and yeast count, thermotolerant coliforms, Staphylococcus aureus, and Pseudomonas aeruginosa. The results are shown in Table 1.

[0080] Table 1 Microbiological detection results

[0081] Testing items unit Test results Limit Total bacterial count CFU / g ＜10 ≤1000 Total number of molds and yeasts CFU / g ＜10 ≤100 Thermoresistant coliforms / g Not detected Not detectable Staphylococcus aureus / g Not detected Not detectable Pseudomonas aeruginosa / g Not detected Not detectable

[0082] Experimental Example 2: Physicochemical Testing

[0083] The anti-wrinkle, repairing, soothing, and moisturizing multi-effect composition prepared in Example 6 was sent to Huace Testing & Certification Group Co., Ltd. for testing of mercury, lead, arsenic, and cadmium. The results are shown in Table 2.

[0084] Table 2. Physicochemical test results

[0085]

[0086] Experimental Example 3 Antioxidant Detection

[0087] The anti-wrinkle, repairing, soothing, and moisturizing multi-effect composition prepared in Example 6 was sent to Huace Testing & Certification Group Co., Ltd. for a free radical scavenging test to detect the antioxidant capacity of the sample.

[0088] (1) Instruments: ELISA reader, analytical balance

[0089] (2) Materials:

[0090] Positive control group: Vitamin C diluted with water to 0.01 mg / mL to verify the test system.

[0091] Samples: The anti-wrinkle, repairing, soothing and moisturizing multi-effect composition prepared in Example 6 was diluted with 95% ethanol to 10% (v / v), 1% (v / v), 0.1% (v / v) and test samples.

[0092] (3) The experimental steps are shown in Table 3.

[0093] Table 3 Sample Liquid Addition Requirements

[0094]

[0095]

[0096] Notes: T - Sample well, T0 - Sample background well, C - Enzyme reaction well, CO - Solvent background well, P - Positive control well, P0 - Positive control background well

[0097] Transfer the ELISA plate into the microplate reader and incubate for 10 minutes. Measure the absorbance at 734 nm.

[0098] (4) Criteria for determining test validity:

[0099] This test is valid if the following conditions are met:

[0100] a) The test system is valid if the clearance rate of vitamin C in the positive control is above 50%.

[0101] b) Calculate the ABTS clearance rate of the sample at each test concentration according to the formula, and simultaneously analyze the standard deviation of clearance rates between parallel tubes in each group.

[0102] Standard Deviation (SD) is required; for a test to be considered validly parallel, the SD value must be ≤5%.

[0103] (5) Results

[0104] The average absorbance and average clearance rates of the samples and positive controls are shown in Table 4.

[0105] Table 4. Average absorbance and average clearance rate of samples and positive controls.

[0106] Group absorbance Clearance C 0.7357±0.0152 0％±1.68％ T-10 0.3499±0.0195 52.44％±2.17％ T-1 0.6800±0.0241 7.58％±2.68％ T-0.1 0.7030±0.0135 4.44％±1.49％ T-0.01 0.2021±0.0108 75.53％±1.20％

[0107] Under the conditions of this test, the clearance rate of vitamin C was 75.53% (≥50%), and the results of the positive control met the standard. Furthermore, the SD values ​​of all parallel tubes in this experiment were ≤5%, thus the parallelism of this experiment was considered valid.

[0108] (6) Conclusion

[0109] Under these conditions, when the sample concentration was 10% (v / v), the scavenging rate of the sample was 52.44%, which means that the sample has the ability to scavenge ABTS free radicals at this concentration and has an antioxidant effect.

[0110] Experiment 4: Efficacy Tests (Moisturizing, Repairing, Soothing, Anti-wrinkle, Gentle and Non-irritating, Suitable for Sensitive Skin)

[0111] The anti-wrinkle, repairing, soothing, and moisturizing multi-effect composition prepared in Example 6 was sent to Huace Testing & Certification Group Co., Ltd. for efficacy testing.

[0112] (1) Test objective

[0113] This test measures the skin's stratum corneum moisture content, transepidermal water loss (TEWL) value, and skin hemoglobin before and after using the test sample in 30 healthy subjects. It also analyzes the skin color (red-green) value (a*), wrinkle length, wrinkle volume, wrinkle area, and wrinkle number, as well as the subjects' self-assessment, to verify the efficacy of the submitted sample in moisturizing, repairing, soothing, anti-wrinkle, gentle and non-irritating, and suitable for sensitive skin.

[0114] (2) Test Plan

[0115] ① Sample usage method

[0116] Application area: Face

[0117] Usage frequency and cycle: Use for 28 days, once in the morning and once in the evening.

[0118] Directions for use: After cleansing, take 2-3 drops of this product and gently massage it onto the face until absorbed.

[0119] ② Subject information

[0120] Subject gender: Female

[0121] Subject age: 28-50 years old

[0122] Number of recruits: 37

[0123] Number of participants screened and enrolled: 37

[0124] Number of people who failed to complete the test: 3

[0125] Valid number of data analysts: 34

[0126] Note: Subjects No. 17, 21 and 23 withdrew from the test midway due to personal reasons.

[0127] ③ Selection Criteria

[0128] Healthy female participants, aged 25-50 years;

[0129] Baseline skin stratum corneum moisture content <60 c.u., baseline transepidermal water loss (TEWL) >15 g / h / m 2Wrinkle grade 1-3; Sensitive skin (lactic acid stinging D0 + subjective questionnaire);

[0130] They can cooperate well with the test subjects and maintain a regular lifestyle during the research period;

[0131] Able to read and understand all contents of the informed consent form and voluntarily sign the informed consent form; agree not to use any cosmetics, drugs, or health products that may affect the results during the trial;

[0132] Other relevant inclusion criteria.

[0133] ④ Exclusion criteria

[0134] Anyone meeting any of the following conditions must be excluded from this study.

[0135] Those with facial skin conditions that may affect their judgment of test results;

[0136] People with severe allergies;

[0137] Women who are pregnant, breastfeeding, or planning to become pregnant during the trial;

[0138] Those with severe heart, liver, or kidney dysfunction and severe immunodeficiency;

[0139] Those with mental illness, severe endocrine disorders, or those taking oral contraceptives;

[0140] Those who have participated in drug clinical trials or other trials within the past 30 days, or those who have systematically used drugs that may affect the trial results within the past week;

[0141] Those who have used oral or topical beauty products within the past two weeks that may affect the test results;

[0142] Unable to cooperate with the test subject;

[0143] Those deemed unsuitable to participate in this study by the researchers;

[0144] Other relevant exclusion criteria.

[0145] ⑤ Exit Criteria

[0146] Patients may withdraw from a clinical study due to complications, adverse events, or other reasons during the study period, but the reason for withdrawal should be stated.

[0147] ⑥ Environmental conditions

[0148] The testing environment conditions throughout the entire testing process were controlled as follows: temperature 20–22℃, humidity 40–60%RH.

[0149] ⑦ Sample processing

[0150] Before testing, laboratory technicians blinded the samples and removed vendor-related information from the sample packaging to avoid affecting the test results. After testing, all test samples were collected.

[0151] (3) Lactic acid stinging test

[0152] ① Subjective evaluation

[0153] On the first day, participants were asked about their sensitivities to climate (cold and dry), skin care products, detergents, textiles, and rough objects, and were given scores for each.

[0154] 0 points indicates insensitivity; 1 point indicates occasional sensitivity; 2 points indicates sensitivity. The scores from all five categories are added together, and those with a total score of ≥4 points proceed to the lactic acid stinging screening.

[0155] Example: Subject A reported being insensitive to climate (0 points), sometimes sensitive to skin care products (1 point), sensitive to detergents (2 points), sometimes sensitive to textiles (1 point), and sometimes sensitive to rough objects (1 point); the five items added together: 0+1+2+1+1=5 points, thus entering the lactic acid stinging screening.

[0156] ② Lactic acid sting test

[0157] 50 μL of 10% lactic acid solution was dropped onto a single layer of nonwoven fabric with a diameter of 0.8 cm. This fabric was then applied to the nasolabial fold on one side of the subject, with distilled water used as a control on the other side. Researchers asked the subjects about their stinging sensation at 0.5 min, 2.5 min, 5 min, and 8 min, and scored them according to Table 5. Subjects with a cumulative stinging sensation of ≥5 points on the lactic acid side relative to the distilled water side were considered to have lactic acid stinging and could be classified as having sensitive skin.

[0158] *Method Reference: Yuan Chao, Wang Xuemin, Tan Yimei, Short-term repair effect of moisturizer on skin barrier in patients with lactic acid stinging[J], Chinese Journal of Aesthetic Medicine, 2011, Vol. 20, No. 7, pp. 1121-1123, ISTIC CA, 2011.

[0159] The lactic acid stinging rating scale is shown in Table 5:

[0160] Table 5 Lactic acid stinging rating scale

[0161] Rating levels Adverse reactions 0 No stinging sensation 1 Slight stinging 2 Moderate stinging pain, tolerable 3 Severe stinging pain, unbearable

[0162] Note: If a subject scores 3 points on the lactate side at any time point during the experiment, the experiment will be terminated, and the subject will be considered to have lactate stinging.

[0163] (4) Subject self-assessment

[0164] A questionnaire survey was conducted 14 days and 28 days after using the sample to evaluate the user's experience after using the sample.

[0165] Table 6 Self-Assessment Questionnaire

[0166]

[0167]

[0168] (5) Test steps

[0169] D0 before using the sample:

[0170] ①Inclusion: Volunteer subjects were recruited according to requirements and signed written informed consent forms. Lactic acid stinging tests were conducted before enrollment, and subjects were questioned about their medical history and health status based on inclusion and exclusion criteria.

[0171] ② Cleanse face and wait: Subjects cleanse their face and dry their skin with a dry facial tissue. They then sit quietly for 30 minutes in a laboratory with a temperature of 21±1℃ and a humidity of 50±10%RH.

[0172] ③ Testing: Laboratory technicians used Visia7 and PRIMOSCR to acquire images of the subjects' faces; and measured the subjects' skin stratum corneum moisture content, transepidermal water loss (TEWL) value, and baseline skin hemoglobin value.

[0173] ④ Sample distribution and usage instructions: Laboratory technicians will guide subjects on how to use the samples, distribute test samples, and provide written sample usage instructions and usage logs.

[0174] D14 after 14 days of sample use / D28 after 28 days of sample use

[0175] ① Cleanse face and wait: Subjects cleanse their face and dry their skin with a dry facial tissue, and sit quietly for 30 minutes in a laboratory with a temperature of 21±1℃ and a humidity of 50±10%RH.

[0176] ② Testing: Laboratory technicians used Visia7 and PRIMOSCR to acquire images of the subjects' faces; and measured the subjects' skin stratum corneum moisture content, transepidermal water loss (TEWL) value, and skin hemoglobin data.

[0177] ③ The subjects completed a self-assessment questionnaire.

[0178] (6) Data Analysis

[0179] ① Descriptive statistics

[0180] The statistical analysis software SPSS was used to perform statistical analysis on the data, and the mean, standard deviation, and rate of change of each parameter at each time point were calculated.

[0181] Calculation formula:

[0182] Rate of change = [(Value after use - Value before use) / Value before use] × 100%

[0183] ② Normality test

[0184] If a normality test is performed on the above means, and the asymptotic significance (two-tailed) value of the normality test is >0.050, then the data series follows a normal distribution.

[0185] ③ Difference Analysis

[0186] Before and after sample comparison: Perform difference analysis on the above means. When both sets of data are normally distributed, use the paired T test to analyze the difference between the two sets of data. When the two sets of data are not normally distributed, use the rank-sum test to analyze the difference between the two sets of data.

[0187] Analysis of participants' self-assessment results: Statistical analysis was conducted using the binomial distribution method, with the expected value of the recognition rate set at 60% (the percentage of participants with a score ≥4). The statistical significance of the survey questions was tested using a 95% confidence interval.

[0188] ④ Evaluation criteria

[0189] When P < 0.010, it indicates that there is a highly significant difference between the two groups of data;

[0190] When 0.010 ≤ P < 0.050, it indicates that there is a significant difference between the two sets of data;

[0191] When P ≥ 0.050, it indicates that there is no significant difference between the two data.

[0192] (7) Criteria for Result Judgment

[0193] ① Moisturizing effect:

[0194] If, at any time point before or after product use, the skin's stratum corneum moisture content significantly increases (P<0.050) or the skin's transepidermal water loss (TEWL) value significantly decreases (P<0.050), the test sample is considered to have moisturizing effects.

[0195] ②Repairing effects:

[0196] If the transepidermal water loss (TEWL) value of the skin shows a significant decrease (P<0.050) at any time point before or after product use, the test sample is considered to have a repairing effect.

[0197] ③ Soothing effect:

[0198] If, at any time point before or after product use, the skin hemoglobin or skin color (red-green) a* value of the sensitive skin subjects showed a significant decrease (P<0.050), the test sample was considered to have a soothing effect.

[0199] ④ Anti-wrinkle effect:

[0200] If, at any time point before or after product use, the number, length, volume, or area of ​​skin wrinkles significantly decreases (P<0.050), the test sample is considered to have anti-wrinkle efficacy.

[0201] ⑤ Gentle, non-irritating, and suitable for sensitive skin:

[0202] The product underwent trial testing on over 30 people with sensitive skin, with long-term normal use (long-term is tentatively defined as 28 days or more, and normal use is defined as use according to the usage method provided by the customer). There were no adverse reactions throughout the entire process, which supports the claims of "mild and non-irritating" and "suitable for sensitive skin".

[0203] result

[0204] 1. Moisture content of the stratum corneum of the skin

[0205] Table 7 Results of the Skin Stratum Corneum Moisture Content Test

[0206]

[0207]

[0208] Parameter description: The higher the measured value, the higher the moisture content of the stratum corneum of the skin.

[0209] Results Analysis: Compared with before sample use, the skin stratum corneum moisture content of the 34 subjects showed a highly significant difference after 14 days of sample use; the skin stratum corneum moisture content also showed a highly significant difference after 28 days of sample use. This indicates that the test sample has a moisturizing effect after 28 days of sample use.

[0210] 2. Transepidermal water loss (TEWL) value of the skin

[0211] Table 8 Results of the Transepidermal Water Loss (TEWL) Value Test

[0212] D0 D14 D28 mean 19.4 17.1 16.0 Standard deviation 3.4 2.8 2.2 rate of change / -11.86％ -17.53％ p-value / ＜0.001 ＜0.001 Difference analysis / There is a highly significant difference There is a highly significant difference

[0213] Parameter description: The smaller the measured value, the less transepidermal water loss per unit time and per unit cross-sectional area.

[0214] Results Analysis: Compared with before sample use, the transepidermal water loss (TEWL) values ​​of the 34 subjects showed a highly significant difference after 14 days of sample use; the TEWL values ​​also showed a highly significant difference after 28 days of sample use. This indicates that the test sample has a repairing effect after 28 days of sample use.

[0215] 3. Skin hemoglobin

[0216] Table 9 Results of the skin heme test

[0217]

[0218]

[0219] Parameter description: The smaller the measured value, the lower the skin hemoglobin content.

[0220] Results Analysis: Compared with before sample use, there was a highly significant difference in skin hemoglobin levels in the 34 subjects after 14 days of sample use; there was also a highly significant difference in skin hemoglobin levels after 28 days of sample use. This indicates that the test sample has a soothing effect after 28 days of sample use.

[0221] 4. Skin color (red-green) value a* value

[0222] Table 10 Results of Skin Color (Red-Green) Values ​​(a*) Test

[0223] D0 D14 D28 mean 27.51 27.68 27.15 Standard deviation 5.84 6.14 6.53 rate of change / 0.62％ -1.31％ p-value / 0.716 0.469 Difference analysis / No significant difference No significant difference

[0224] Parameter description: The smaller the measured value, the less red the skin color.

[0225] Results analysis: Compared with before sample use, there was no significant difference in skin color (red-green) a* value among the 34 subjects after 14 days of sample use; there was also no significant difference in skin color (red-green) a* value after 28 days of sample use.

[0226] 5. Length of skin wrinkles

[0227] Table 11 Results of the Skin Wrinkle Length Test

[0228]

[0229]

[0230] Parameter description: The smaller the measured value, the shorter the wrinkle length.

[0231] Result Analysis:

[0232] At the corner of the eye: Compared with before using the sample, the length of skin wrinkles in 34 subjects was significantly different after 14 days of using the sample; the length of skin wrinkles was significantly different after 28 days of using the sample.

[0233] Under-eye area: Compared with before sample use, the length of skin wrinkles in 34 subjects was significantly different after 14 days of sample use; the length of skin wrinkles was significantly different after 28 days of sample use.

[0234] This indicates that the test sample had anti-wrinkle effects after 28 days of use.

[0235] 6. Volume of skin wrinkles

[0236] Table 12 Results of Skin Wrinkle Volume Test

[0237]

[0238] Parameter description: The smaller the measured value, the smaller the wrinkle volume. Result interpretation:

[0239] Corner of the eye area: Compared with before the use of the sample, there was a highly significant difference in skin wrinkle volume in 34 subjects after 14 days of sample use; there was a significant difference in skin wrinkle volume after 28 days of sample use.

[0240] Under-eye area: Compared with before sample use, there was no significant difference in skin wrinkle volume among the 34 subjects after 14 days of sample use; there was no significant difference in skin wrinkle volume after 28 days of sample use.

[0241] This indicates that the test sample had anti-wrinkle effects after 28 days of use.

[0242] 7. Area of ​​skin wrinkles

[0243] Table 13 Results of the Skin Wrinkle Area Test

[0244]

[0245] Parameter description: The smaller the measured value, the smaller the wrinkle area.

[0246] Result Analysis:

[0247] Around the eyes: Compared with before using the sample, the area of ​​skin wrinkles in the 34 subjects was significantly different after 14 days of using the sample; the area of ​​skin wrinkles was significantly different after 28 days of using the sample.

[0248] Under-eye area: Compared with before sample use, the area of ​​skin wrinkles in 34 subjects was significantly different after 14 days of sample use; the area of ​​skin wrinkles was significantly different after 28 days of sample use.

[0249] This indicates that the test sample had anti-wrinkle effects after 28 days of use.

[0250] 8. Number of skin wrinkles

[0251] Table 14 Results of the Skin Wrinkle Count Test

[0252]

[0253]

[0254] Parameter description: The smaller the measurement value, the fewer the wrinkles.

[0255] Result Analysis:

[0256] Around the eyes: Compared with before using the sample, there was a highly significant difference in the number of skin wrinkles among the 34 subjects after 14 days of sample use; there was also a highly significant difference in the number of skin wrinkles after 28 days of sample use.

[0257] Under-eye area: Compared with before sample use, the number of skin wrinkles in 34 subjects was significantly different after 14 days of sample use; the number of skin wrinkles was significantly different after 28 days of sample use.

[0258] This indicates that the test sample had anti-wrinkle effects after 28 days of use.

[0259] 9. Subject self-assessment rating criteria:

[0260] Questions 1-17: (out of 5) 1 point is strongly disagree, 2 points is disagree, 3 points is indifferent to agree or disagree, 4 points is agree, and 5 points is strongly agree.

[0261] Questions 18-19: (2-point scale) 1 point for disagreement, 2 points for agreement.

[0262] Agreement statistics:

[0263] Approval Rating = [(Number of people who rated 4 points (agree) + Number of people who rated 5 points (strongly agree)) / Total Number of People] × 100%

[0264] Table 15: Subject Self-Assessment Results and Level of Agreement

[0265]

[0266]

[0267]

[0268] Test results and efficacy conclusions:

[0269] (1) This test sample showed moisturizing effects after 28 days of use. This test sample showed repairing effects after 28 days of use. This test sample showed soothing effects after 28 days of use. This test sample showed anti-wrinkle effects after 28 days of use.

[0270] By observing 34 female subjects aged 28-50 with sensitive skin, who used the test sample continuously for 28 days under normal conditions, the results showed that none of the 34 subjects experienced adverse reactions after using the sample for 28 days. Based on these test results, the customer's claims that the product is "suitable for sensitive skin" and "mild and non-irritating" can be supported.

[0271] (2) Consumer conclusion:

[0272] The subjects acknowledged that the product had moisturizing, soothing, and anti-wrinkle effects, which supports the product's claim of "moisturizing, soothing, and anti-wrinkle effects".

[0273] (3) No adverse reactions occurred in any of the 34 subjects during the entire test.

[0274] Test Example 5: Occlusive Patch Test

[0275] The anti-wrinkle, repairing, soothing, and moisturizing multi-effect composition prepared in Example 6 was sent to Huace Testing & Certification Group Co., Ltd. for a skin occlusive patch test.

[0276] (1) Test method

[0277] Select an area not exceeding 50mm 2 A qualified spot treatment device with a depth of 1 mm is required. The test substance is added to the spot treatment device at a volume of 0.020 mL to 0.025 mL (liquid). The spot treatment device containing the test substance and negative control is applied to the flexor side of the subject's forearm. The spot treatment device is removed after 24 hours. Skin reactions are observed according to the standards in Table 16 at 30 minutes (after the indentation disappears), 24 hours, and 48 hours after removal of the spot treatment device, and the results are recorded.

[0278] Table 16 Grading Criteria for Skin Reactions in Closed Patch Tests

[0279]

[0280] (2) Test Results: A total of 33 subjects were included in this test, and 32 subjects completed the trial (subject 4 was excluded due to adverse reactions in the negative control area). Among them, there were 5 males and 27 females, aged 23 to 57 years. The results of this test showed that all 32 subjects had a score of 0 at 0.5h, 24h, and 48h after removal of the test substance plaque, indicating no adverse reactions.

[0281] Table 17 Results of Skin Occlusive Patch Test

[0282]

[0283] The test results showed that all 32 subjects had a score of 0 at 0.5h, 24h, and 48h after the removal of the test substance plaque, indicating no adverse reactions.

[0284] Table 18. Analysis of Subject Age

[0285] Minimum value Maximum value mean Standard deviation age 23 57 29.9 7.7

[0286] As can be seen from the above embodiments, the present invention provides a multi-effect composition for anti-wrinkle repair, soothing, and moisturizing, and its application. The composition comprises the following raw materials in parts by weight: 1-10 parts of agarwood extract and 0.1-2 parts of bisabolol. The product of the present invention uses agarwood extract as the main active ingredient, has a simple preparation process, and exhibits excellent effects. It can be used on sensitive skin, possessing anti-wrinkle, repairing, soothing, and moisturizing effects, and also has antioxidant effects, effectively scavenging ABTS free radicals and delaying skin aging.

[0287] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A multi-effect composition for anti-wrinkle, repair, soothing, and moisturizing, characterized in that, The raw materials include the following parts by weight: 1-10 parts of agarwood extract and 0.1-2 parts of bisabolol.

2. The multi-effect composition according to claim 1, characterized in that, The preparation method of the agarwood extract includes the following steps: (1) Crush the raw agarwood into powder to obtain agarwood fragments; (2) Distill the agarwood fragments to obtain the distillate; (3) The distillate was separated into oil and water to obtain agarwood extract.

3. The multi-effect composition according to claim 2, characterized in that, The content of sesquiterpenoid compounds in the agarwood raw material in step (1) is ≥60%, the particle size of the agarwood fragments is >50 mesh, and the distillation in step (2) is carried out by steam distillation, the distillation temperature is 95-105℃, and the distillation time is 44-52h.

4. The multi-effect composition according to any one of claims 1 to 3, characterized in that, The multi-effect composition also contains fractionated coconut oil, and the mass ratio of the agarwood extract, bisabolol and fractionated coconut oil is 1-10:0.1-2:88-98.

9.

5. The method for preparing the multi-effect composition according to claim 4, characterized in that, Includes the following steps: (1) Weigh the raw materials according to the mass ratio of 1-10 parts of agarwood extract, 0.1-2 parts of bisabolol, and 88-98.9 parts of fractionated coconut oil; (2) Agarwood extract, bisabolol and fractionated coconut oil are mixed and kept warm to obtain the multi-effect composition.

6. The preparation method according to claim 5, characterized in that, The mixing temperature in step (2) is 28-32°C, the mixing time is 18-22 min, the rotation speed during the mixing process is 40-50 revolutions per minute, and the heat preservation time is 15-25 min.

7. The use of the multi-effect composition according to any one of claims 1 to 4 in the preparation of anti-wrinkle, repairing, soothing and moisturizing products.

8. A product for anti-wrinkle repair, soothing, and moisturizing, characterized in that, The product contains any one of the multi-effect compositions described in 1 to 4.

9. The product according to claim 8, characterized in that, The products also include cosmetic-grade excipients, and the types of products include face creams, lotions, face masks, eye creams, eye serums, neck creams, and body oils.

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