Portable cell incubator

Abstract

The invention belongs to the technical field of cell culture, and relates to a portable cell culture box. The invention provides a portable cell culture box which can keep overall stability and can control the concentration and temperature of carbon dioxide in the box so as to facilitate long-distance transportation. The portable cell culture box comprises a box body, a control panel, a box cover, a bottom plate, a carbon dioxide pressure tank, a battery and the like, a control panel is installed on the front side of the upper portion of the box body, a box cover is hinged to the top of the box body, a bottom plate is connected to the bottom of the box body, and a carbon dioxide pressure tank used for keeping the carbon dioxide concentration and a battery located right behind the carbon dioxide pressure tank are installed on the upper side of the left front portion of the bottom plate. Through the anti-shake mechanism similar to the chicken head principle, the frame body is kept stable relative to the box body, the carbon dioxide concentration in the box body is kept at 5% through the carbon dioxide pressure tank, and the battery supplies power, so that long-distance cell transportation is facilitated.

Description

[0001] This application is a divisional application of the invention patent application with application number CN202210981618.5 and invention title: Portable Cell Culture Box, with the parent application date being August 15, 2022. Technical Field

[0002] This invention belongs to the field of cell culture technology and relates to a portable cell culture box. Background Technology

[0003] There is no universally accepted definition of a cell, but a common understanding is that it is the basic structural and functional unit of an organism. It is known that all organisms, except viruses, are composed of cells, but viral life activities can only be manifested within cells. To simulate the in vivo environment in vitro, experiments are conducted using cell culture methods. Cell culture requires cell culture equipment. Currently, commercially available cell culture incubators often suffer from instability when transported, with the culture bottles becoming unstable and shaking as the incubator moves, making it impossible to stably culture the cells within.

[0004] For example, a portable cell culture box disclosed in patent publication number CN211284398U, published on August 18, 2020, includes a protective outer box, anti-slip feet, an auxiliary buffer frame structure, an internal storage frame, a horizontal partition, an L-shaped storage plate, side rubber pads, a lid, an L-shaped plate, a tightening bolt, and a side carrying frame structure. The anti-slip feet are glued to the lower four corners of the protective outer box; the auxiliary buffer frame structure is installed on the lower inner side of the protective outer box; and the internal storage frame is placed inside the protective outer box.

[0005] While the aforementioned portable cell culture chamber can provide shock absorption when accidentally dropped on the ground thanks to its bottom-mounted auxiliary buffer structure, this structure only cushions the impact from below and cannot maintain overall stability. Furthermore, it cannot control the carbon dioxide concentration inside the chamber, limiting its ability to transport cells over long distances.

[0006] In view of the shortcomings of the existing technology, a portable cell culture box is designed to overcome the shortcomings of the existing technology, maintain overall stability, control the carbon dioxide concentration and temperature inside the box, and thus facilitate long-distance transportation. Summary of the Invention

[0007] In order to overcome the limitations of the existing technology, which cannot maintain overall stability and cannot control the carbon dioxide concentration inside the chamber, thus limiting the long-distance transport of cells, the present invention aims to provide a portable cell culture chamber that can maintain overall stability and control the carbon dioxide concentration and temperature inside the chamber, thereby facilitating long-distance transport.

[0008] To address the aforementioned technical problems, the present invention provides a portable cell culture chamber, comprising: The chamber has a control panel installed on the upper front side for displaying carbon dioxide concentration, air humidity, temperature, and ion count. The top of the chamber is hinged with a lid, and the bottom of the chamber is connected to a base plate. A carbon dioxide pressure tank for maintaining carbon dioxide concentration and a battery located directly behind the carbon dioxide pressure tank are installed on the upper left front side of the base plate. The carbon dioxide pressure tank is electrically connected to the battery. The carbon dioxide concentration can be controlled through the carbon dioxide pressure tank to maintain the carbon dioxide concentration at 5%. The anti-shake mechanism, mounted on the base plate, is designed based on the principle of a chicken's head. It includes a frame, magnets, pads, shock-absorbing springs, and rubber plugs. The frame is located on top of the base plate, with magnets connected to both the bottom and top sides of the frame and the base plate. These magnets repel each other. Pads are located on all four sides of the frame, and shock-absorbing springs connect the frame and base plate to these pads. A delivery pipe connects the carbon dioxide pressure tank to the frame, allowing the pressure tank to regulate the carbon dioxide concentration within the frame. A filter screen is connected to the delivery pipe on the frame to filter bacteria from the carbon dioxide delivered through the pipe. A rubber plug that engages with the frame is attached to the bottom of the lid. The repulsion between the two magnets suspends the frame, and the shock-absorbing springs connect the pads to the base plate and the frame to the pads, thus achieving the effect of cushioning and shock absorption. A clamping mechanism is provided on the anti-shake mechanism. The clamping mechanism includes a support plate, a two-way lead screw, and a clamping plate. The support plate is connected to the lower inner side of the frame. The two-way lead screw is rotatably provided on the upper part of the support plate. The front and rear ends of the two-way lead screw are threadedly connected to clamping plates that slide with the support plate. A culture dish, wherein the culture dish is clamped between two clamping plates on the same bidirectional screw for loading cells; A lifting mechanism is used to eject the culture dish. The lifting mechanism includes a sliding plate, a compression spring, a T-shaped rod, and contact rods. A sliding plate, located directly below a support plate, is slidably mounted on the lower inner side of the frame. A compression spring connects the sliding plate to the frame. A T-shaped rod for pushing the culture dish is connected to the top center of the sliding plate. Contact rods are connected to the four upper corners of the sliding plate. Rubber plugs contact the contact rods. In the closed state, the rubber plugs compress the contact rods, and the compression spring is compressed. In the open state, the rubber plugs rotate, releasing the contact rods, and the compression spring gradually returns to its original position. The sliding plate moves upward under the action of the returning compression spring. This will cause the T-shaped rod to move upward, lifting the petri dish. When multiple petri dishes are stacked, the topmost petri dish will move out of the limit position. When the topmost petri dish is removed, the weight of the slide plate decreases, the compression spring returns to its original position, and the next petri dish will then move out. The petri dish moves upward automatically and synchronously when the device is opened. When the device needs to be closed, the contact rod moves downward, which in turn moves the slide plate downward. The compression spring is compressed, and the downward movement of the slide plate moves the T-shaped rod downward. Finally, the rubber stopper rotates in the opposite direction and presses against the contact rod, thus preparing for the next petri dish ejection operation.

[0009] Preferably, the culture dish includes a container body, a lid, and a bacterial filter. The container body for loading cells is held between two clamps on the same bidirectional screw. The left part of the container body extends upward and to the left to form a tube arm for transporting cells into the container body. A bacterial filter is installed at the upper left opening of the tube arm, and a lid is threadedly connected to the upper left opening of the tube arm. Rotating the bidirectional screw causes the clamps on both sides to move away from each other and release the container body. Then, the container body is removed from between the two clamps and placed back between the two clamps. Rotating the bidirectional screw in the opposite direction causes the clamps on both sides to move closer together and clamp the container body again, thereby fixing the container body.

[0010] Preferably, it also includes a limiting plate and a buffer spring. Two limiting plates are slidably arranged in a front-to-back manner on the upper part of the frame. A buffer spring is connected between the limiting plate and the frame. The side of the two limiting plates that are close to each other is arc-shaped. The limiting plates are used to limit the culture dish.

[0011] Preferably, the bottom side of the container body forms an upward-facing depression, and the top side of the container body forms an upward-facing protrusion. The protrusions and depressions of multiple containers engage with each other to stably overlap and be placed between two limiting plates. When the containers are stacked vertically, the protrusions and depressions of multiple culture dishes engage with each other to stably overlap and be placed between two limiting plates. The limiting plates will limit the overlapping culture dishes under the action of buffer springs, thereby ensuring the stability of the culture dishes while allowing multiple culture dishes to be placed.

[0012] Preferably, it also includes a thermoelectric cooler, the limiting plate is made of metal, and a thermoelectric cooler for temperature control is installed on the side of the two limiting plates that are close to each other. The thermoelectric cooler is electrically connected to the battery.

[0013] Preferably, it also includes a water tank and an electric spray head. Two symmetrically arranged water tanks are connected to the upper rear inner side of the housing, and an electric spray head embedded in the frame is connected to the front side of the water tank. The electric spray head is used to control the humidity inside the frame.

[0014] In addition to overcoming the shortcomings of existing technologies, this invention also achieves the following beneficial effects: 1. Through a shock-absorbing mechanism similar to that of a chicken head, the frame remains stable relative to the box. A carbon dioxide pressure tank keeps the carbon dioxide concentration inside the box at 5%. Battery powered, this facilitates long-distance cell transport.

[0015] 2. When the containers are stacked vertically, the limiting plate will limit the stacked culture dishes under the action of the buffer spring, thus ensuring the stability of the culture dishes and allowing multiple culture dishes to be placed, which improves the practicality of the device.

[0016] 3. The compression spring automatically and synchronously moves the culture dish upwards when the device is opened, making it easier for the operator to remove the culture dish and improving the synchronization of the device.

[0017] 4. The electric spray head will spray water from the water tank into the frame in the form of atomization, thereby increasing the humidity inside the frame.

[0018] 5. The temperature inside the cabinet is controlled by heating or cooling the limiting plate using a semiconductor cooling chip, thereby facilitating cell culture operations. Attached Figure Description

[0019] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0020] Figure 2 This is a cross-sectional view of the present invention.

[0021] Figure 3 This is a partial three-dimensional structural schematic diagram of the present invention.

[0022] Figure 4 This is a three-dimensional structural diagram of the image stabilization mechanism of the present invention.

[0023] Figure 5 This is a partial three-dimensional structural diagram of the anti-shake mechanism of the present invention, wherein the frame is fully sectional.

[0024] Figure 6 This is a partial three-dimensional structural diagram of the clamping mechanism of the present invention.

[0025] Figure 7 This is a three-dimensional structural diagram of the clamping mechanism of the present invention.

[0026] Figure 8 This is a partial three-dimensional structural diagram of the petri dish of the present invention.

[0027] Figure 9 This is a cross-sectional view of the petri dish of the present invention.

[0028] Figure 10 This is a diagram showing the connection relationship between the water tank and the electric spray head, etc., of the present invention.

[0029] Figure 11 This is a diagram showing the connection relationships of components such as the semiconductor cooling chip of the present invention.

[0030] Figure 12 This is a partial three-dimensional structural diagram of the lifting mechanism of the present invention.

[0031] Figure 13 This is a three-dimensional structural diagram of the lifting mechanism of the present invention.

[0032] The labels in the attached diagram are as follows: 1-Box body, 2-Control panel, 3-Box lid, 4-Base plate, 5-Carbon dioxide pressure tank, 6-Battery, 7-Anti-shake mechanism, 71-Frame, 72-Magnet, 73-Padded plate, 74-Shock-absorbing spring, 75-Rubber stopper, 8-Clamping mechanism, 81-Support plate, 82-Double-actuated screw, 83-Clamping plate, 9-Cultural dish, 91-Container body, 92-Lid, 93-Bacterial filter, 101-Limiting plate, 102-Buffer spring, 11-Lifting mechanism, 111-Slide plate, 112-Compression spring, 113-T-shaped rod, 114-Contact rod, 12-Semiconductor cooling chip, 13-Water tank, 131-Electric spray head, 14-Filter screen. Detailed Implementation

[0033] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0034] Example 1 A portable cell culture incubator, such as Figure 1-3As shown, the device includes a housing 1, a control panel 2, a lid 3, a base plate 4, a carbon dioxide pressure tank 5, a battery 6, a shock-absorbing mechanism 7, a clamping mechanism 8, and a culture dish 9. The control panel 2 is bolted to the upper front of the housing 1. The lid 3 is hinged to the top of the housing 1. The base plate 4 is bolted to the bottom of the housing 1. The carbon dioxide pressure tank 5, which is used to maintain the carbon dioxide concentration, and the battery 6, located directly behind the carbon dioxide pressure tank 5, are bolted to the upper left front of the base plate 4. The carbon dioxide pressure tank 5 and the battery 6 are electrically connected. The base plate 4 is equipped with a shock-absorbing mechanism 7 for buffering and shock reduction. The shock-absorbing mechanism 7 is equipped with a clamping mechanism 8. The clamping mechanism 8 is equipped with a culture dish 9 for loading cells.

[0035] Working Principle: The operator can apply the corresponding technical solutions in this device to the portable cell culture box according to specific circumstances. Initially, the device is in a closed state. When it is necessary to use this device to assist in cell culture operations, firstly, the operator rotates the lid 3 to open the device, then removes the culture dish 9 from the clamping mechanism 8, and then injects the cells to be cultured into the culture dish 9. After injection, the operator puts the culture dish 9 back onto the clamping mechanism 8, which clamps the culture dish 9 to prevent displacement. Then, the operator rotates the lid 3 in the opposite direction to close the device, and then the cell culture operation can begin. Panel 2 displays the carbon dioxide concentration, air humidity, temperature, and ion count inside the frame 71 for the operator's convenience. The carbon dioxide pressure tank 5 controls the carbon dioxide concentration inside the frame 71, maintaining it at 5%. Due to its enclosed structure, the operator can easily carry the device, making it portable. During transport, the anti-shake mechanism 7, designed according to the chicken head principle, keeps the frame 71 stable, providing cushioning and shock absorption to prevent excessive shaking from affecting cell culture operations. Battery 6 powers the control panel 2 and the carbon dioxide pressure tank 5.

[0036] Example 2 Based on Example 1, such as Figure 4 and Figure 5As shown, the anti-shake mechanism 7 includes a frame 71, magnets 72, pads 73, shock-absorbing springs 74, and rubber plugs 75. The frame 71 is located above the base plate 4. Magnets 72 are bolted to the bottom side of the frame 71 and the top side of the base plate 4. The two magnets 72 repel each other. Pads 73 are provided on the four sides of the frame 71. Shock-absorbing springs 74 are connected between the frame 71 and the base plate 4 and the pads 73. A delivery pipe is connected between the carbon dioxide pressure tank 5 and the frame 71. The carbon dioxide pressure tank 5 adjusts the carbon dioxide concentration in the frame 71 through the delivery pipe. A filter screen 14 is connected to the position of the frame 71 where the delivery pipe is connected. The filter screen 14 is used to filter bacteria in the carbon dioxide delivered by the delivery pipe. A rubber plug 75 is connected to the lower side of the lid 3 and engages with the frame 71.

[0037] like Figure 6 and Figure 7 As shown, the clamping mechanism 8 includes a support plate 81, a bidirectional lead screw 82, and a clamping plate 83. The support plate 81 is bolted to the lower inner side of the frame 71. Four bidirectional lead screws 82 are rotatably arranged at left and right intervals on the upper part of the support plate 81. The front and rear ends of the bidirectional lead screw 82 are threaded with clamping plates 83 that slide with the support plate 81. The culture dish 9 is clamped between two clamping plates 83 on the same bidirectional lead screw 82.

[0038] like Figures 7-9 As shown, the petri dish 9 includes a container body 91, a lid 92, and a bacterial filter 93. The container body 91 for loading cells is held between two clamps 83 on the same bidirectional screw 82. The left part of the container body 91 extends to the upper left to form a tube arm for transporting cells into the container body 91. The bacterial filter 93 is installed at the upper left opening of the tube arm, and the lid 92 is threadedly connected to the upper left opening of the tube arm.

[0039] Initially, two clamps 83 on the same bidirectional screw 82 hold the container 91. The operator rotates the bidirectional screw 82, causing it to move the clamps 83 away from each other, releasing the container 91. The operator then removes the container 91 from between the clamps 83, unscrews the cap 92 from the tube arm, and injects cells into the container 91 through the tube arm. The bacterial filter 93 filters the bacteria. The cap 92 is then screwed back onto the tube arm, completing the cell injection operation. The operator then places the container 91 back between the two clamping plates 83 and rotates the bidirectional lead screw 82 in the opposite direction, causing the bidirectional lead screw 82 to drive the clamping plates 83 on both sides to come closer together and clamp the container 91 again, thus fixing the container 91. Then, during the transfer of this device, the two magnets 72 repel each other, causing the frame 71 to levitate. The pad 73 is connected to the base plate 4 by the shock-absorbing spring 74, and the frame 71 is connected to the pad 73 by the shock-absorbing spring 74, thus achieving the effect of buffering and shock absorption.

[0040] Example 3 Based on Example 2, such as Figure 6 As shown, it also includes a limiting plate 101 and a buffer spring 102. Two limiting plates 101 are slidably arranged in front and behind on the upper part of the frame 71. A buffer spring 102 is connected between the limiting plate 101 and the frame 71. The sides of the two limiting plates 101 that are close to each other are arc-shaped. The limiting plate 101 is used to limit the culture dish 9.

[0041] In a preferred embodiment, the bottom side of the container body 91 is recessed upwards, and the top side of the container body 91 is protruded upwards. The protrusions and recesses of the multiple container bodies 91 are interlocked and fitted together to be stably stacked between the two limiting plates 101.

[0042] The operator places the container bodies 91 vertically stacked together, with the protrusions and recesses of multiple culture dishes 9 interlocking to stably stack the culture dishes 9 between two limiting plates 101. The limiting plates 101, under the action of the buffer spring 102, limit the stacked culture dishes 9, thereby ensuring the stability of the culture dishes 9 and allowing multiple culture dishes 9 to be placed, thus improving the practicality of the device.

[0043] Example 4 Based on Example 3, such as Figure 12 and Figure 13 As shown, it also includes a lifting mechanism 11 for pushing the petri dish 9 out. The lifting mechanism 11 includes a sliding plate 111, a compression spring 112, a T-shaped rod 113 and a contact rod 114. The sliding plate 111 located directly below the support plate 81 is slidably provided on the lower inner side of the frame 71. The compression spring 112 is connected between the sliding plate 111 and the frame 71. Four T-shaped rods 113 arranged symmetrically on the left and right and used to push the petri dish 9 are bolted to the top side of the middle part of the sliding plate 111. Contact rods 114 are bolted to the four corners of the upper side of the sliding plate 111. The rubber plug 75 contacts the contact rod 114.

[0044] Initially, the device is in the closed state, with the rubber stopper 75 pressing against the contact rod 114, and the compression spring 112 is in a compressed state. When the device is opened, the rotation of the rubber stopper 75 releases the contact rod 114, and the compression spring 112 gradually returns to its original position. The sliding plate 111 moves upward under the action of the returning compression spring 112. The upward movement of the sliding plate 111 drives the T-shaped rod 113 to move upward, and the upward movement of the T-shaped rod 113 lifts the culture dish 9. Thus, when multiple culture dishes 9 are stacked, the topmost culture dish 9 will move out of the limit position. Then, when the operator removes the topmost culture dish 9, the sliding plate 111... As the gravity of plate 11 decreases, the compression spring 112 returns to its original position, and the next culture dish 9 will then move out from the limiting plate 101. This allows the culture dish 9 to move upward automatically and synchronously while the device is being opened, making it easier for the operator to remove the culture dish 9. When the device needs to be closed, the operator moves the contact rod 114 downward, which in turn moves the slide plate 111 downward. The compression spring 112 is compressed, and the slide plate 111 moves downward, which in turn moves the T-shaped rod 113 downward. Finally, the rubber stopper 75 rotates in the opposite direction to press the contact rod 114, thus preparing for the next culture dish 9 ejection operation.

[0045] Example 5 Based on Example 4, such as Figure 11 As shown, it also includes a semiconductor cooling chip 12. The limiting plate 101 is made of metal. The semiconductor cooling chip 12 for temperature control is installed on the side of the two limiting plates 101 that are close to each other by bolt connection. The semiconductor cooling chip 12 is electrically connected to the battery 6.

[0046] It also includes a water tank 13 and an electric spray head 131. Two symmetrically arranged water tanks 13 are connected to the upper rear inner side of the housing 1. The front side of the water tank 13 is connected to an electric spray head 131 embedded inside the frame 71. The electric spray head 131 is used to control the humidity inside the frame 71.

[0047] The operator injects water into the water tank 13 and then starts the electric spray head 131. The electric spray head 131 sprays the water in the water tank 13 into the frame 71 in the form of atomization, thereby increasing the humidity inside the frame 71. The limiting plate 101 is heated or cooled by the semiconductor cooling chip 12 to control the temperature inside the cabinet, so that the cell culture operation can be carried out smoothly. The battery 6 provides power to the electric spray head and the semiconductor cooling chip 12, making it convenient to carry out when going out.

[0048] The embodiments described above are merely preferred embodiments of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications, improvements, and substitutions without departing from the concept of the present invention, and these all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A portable cell culture incubator, characterized in that, include: The box (1) has a control panel (2) installed on the upper front side of the box (1) for displaying carbon dioxide concentration, air humidity, temperature and ion count. The top of the box (1) is hinged with a box cover (3). The bottom of the box (1) is connected to a base plate (4). The upper left front side of the base plate (4) is equipped with a carbon dioxide pressure tank (5) for maintaining carbon dioxide concentration and a battery (6) located directly behind the carbon dioxide pressure tank (5). The carbon dioxide pressure tank (5) is electrically connected to the battery (6). The carbon dioxide concentration can be controlled through the carbon dioxide pressure tank (5) to keep the carbon dioxide concentration at five percent. The anti-shake mechanism (7) is set on the base plate (4) for buffering and shock absorption. It is designed based on the chicken head principle. The anti-shake mechanism (7) includes a frame (71), magnets (72), pads (73), shock-absorbing springs (74) and rubber plugs (75). The frame (71) is provided on the top of the base plate (4). Magnets (72) are connected to the bottom side of the frame (71) and the top side of the base plate (4). The two magnets (72) repel each other. Pads (73) are provided on the front, back, left and right sides of the frame (71). Shock-absorbing springs (74) are connected between the frame (71) and the base plate (4) and the pads (73). The carbon dioxide pressure tank (5) is connected to the frame. The bodies (71) are connected by a delivery pipe. The carbon dioxide pressure tank (5) adjusts the carbon dioxide concentration in the frame (71) through the delivery pipe. A filter screen (14) is connected to the position where the delivery pipe is connected to the frame (71). The filter screen (14) is used to filter bacteria in the carbon dioxide delivered by the delivery pipe. A rubber plug (75) that engages with the frame (71) is connected to the lower side of the box cover (3). The frame (71) is suspended by the mutual repulsion of two magnets (72). The pad (73) is connected to the base plate (4) by the shock-absorbing spring (74). The frame (71) is connected to the pad (73) by the shock-absorbing spring (74), thereby achieving the effect of buffering and shock absorption. A clamping mechanism (8) is provided on the anti-shake mechanism (7). The clamping mechanism (8) includes a support plate (81), a two-way screw (82) and a clamping plate (83). The support plate (81) is connected to the lower inner side of the frame (71). The two-way screw (82) is rotatably provided on the upper part of the support plate (81). The front and rear ends of the two-way screw (82) are threadedly connected to the clamping plate (83) which slides with the support plate (81). A culture dish (9) is held between two clamping plates (83) on the same bidirectional screw (82) for loading cells; A lifting mechanism (11) is used to push out the culture dish (9). The lifting mechanism (11) includes a sliding plate (111), a compression spring (112), a T-shaped rod (113), and a contact rod (114). The sliding plate (111) located directly below the support plate (81) is slidably provided on the lower inner side of the frame (71). The compression spring (112) is connected between the sliding plate (111) and the frame (71). The middle top side of the sliding plate (111) A T-shaped rod (113) for pushing the petri dish (9) is connected. Contact rods (114) are connected to the four upper corners of the slide plate (111). A rubber stopper (75) contacts the contact rods (114). In the closed state, the rubber stopper (75) presses against the contact rods (114), and the compression spring (112) is compressed. When opened, the rubber stopper (75) rotates to release the contact rods (114), and the compression spring (112) gradually returns to its original position. The slide plate (111) is compressed... The spring (112) moves upward under the action of resetting, and the slide plate (111) moves upward, which will drive the T-shaped rod (113) to move upward. The T-shaped rod (113) will lift the petri dish (9) so that when multiple petri dishes (9) are stacked, the topmost petri dish (9) will move out from the limit. When the topmost petri dish (9) is removed, the weight of the slide plate (111) decreases, the compression spring (112) resets, and the next petri dish (9) will then move out. When the device is opened, the petri dish (9) moves upward automatically and synchronously. When the device needs to be closed, the contact rod (114) moves downward, and the contact rod (114) will drive the slide plate (111) to move downward. The compression spring (112) is compressed, and the slide plate (111) moves downward, which will drive the T-shaped rod (113) to move downward. Finally, the rubber stopper (75) rotates in the opposite direction to squeeze the contact rod (114), thus preparing for the next petri dish (9) ejection operation.

2. The portable cell culture box according to claim 1, characterized in that, The culture dish (9) includes a container body (91), a lid (92), and a bacterial filter (93). The container body (91) for loading cells is held between two clamps (83) on the same bidirectional screw (82). The left part of the container body (91) extends to the upper left to form a tube arm for transporting cells into the container body (91). A bacterial filter (93) is installed at the upper left opening of the tube arm, and a lid (92) is threaded to the upper left opening of the tube arm. Rotating the bidirectional screw (82) causes the clamps (83) on both sides to move away from each other and release the container body (91). Then, the container body (91) is removed from between the two clamps (83), and the container body (91) is placed back between the two clamps (83). Rotating the bidirectional screw (82) in the opposite direction causes the clamps (83) on both sides to move closer together and clamp the container body (91) again, thereby fixing the container body (91).

3. A portable cell culture box according to claim 2, characterized in that, It also includes a limiting plate (101) and a buffer spring (102). Two limiting plates (101) are slidably arranged in front and behind on the upper part of the frame (71). A buffer spring (102) is connected between the limiting plate (101) and the frame (71). The two limiting plates (101) are arc-shaped on the side that is close to each other. The limiting plate (101) is used to limit the culture dish (9).

4. A portable cell culture box according to claim 3, characterized in that, The bottom side of the container (91) is concave upwards, and the top side of the container (91) is convex upwards. The convex and concave parts of multiple containers (91) are interlocked to stably overlap between two limiting plates (101). The containers (91) are stacked vertically together, and the convex and concave parts of multiple culture dishes (9) are interlocked to stably overlap between two limiting plates (101). The limiting plates (101) will limit the overlapping culture dishes (9) under the action of the buffer spring (102), thereby ensuring the stability of the culture dishes (9) and allowing multiple culture dishes (9) to be placed.

5. A portable cell culture box according to claim 4, characterized in that, It also includes a thermoelectric cooler (12), the limiting plate (101) is made of metal, and thermoelectric coolers (12) for temperature control are installed on the side of the two limiting plates (101) that are close to each other. The thermoelectric cooler (12) is electrically connected to the battery (6).

6. A portable cell culture box according to claim 5, characterized in that, It also includes a water tank (13) and an electric spray head (131). The upper rear inner side of the box (1) is connected to two symmetrically arranged water tanks (13). The front side of the water tank (13) is connected to an electric spray head (131) embedded in the frame (71). The electric spray head (131) is used to control the humidity inside the frame (71).

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