Green extraction method of lycium barbarum polysaccharide
By using segmented enzymatic hydrolysis and activated carbon adsorption, the problem of low yield of wolfberry polysaccharides was solved, achieving efficient extraction of wolfberry polysaccharides, improving yield and purity, and reducing production costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-05
- Publication Date
- 2026-04-14
AI Technical Summary
The extraction yield of wolfberry polysaccharides in existing technologies is insufficient, resulting in low raw material utilization, resource waste, and high product costs.
Goji berry powder was treated with a segmented enzymatic hydrolysis method combined with a complex enzyme (cellulase, pectinase and xylanase), followed by multiple adsorption treatments with activated carbon of different particle sizes, including wood powder and coconut shell activated carbon. Finally, goji berry polysaccharides were obtained by alcohol precipitation and centrifugation.
It significantly improved the yield and purity of wolfberry polysaccharides, reduced impurities, and enhanced raw material utilization and product competitiveness.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of polysaccharide extraction technology, specifically to a green extraction method for wolfberry polysaccharides. Background Technology
[0002] As the core active ingredient of wolfberry, wolfberry polysaccharides have expanded their application scenarios from traditional health products to multiple high-value-added fields such as functional foods, pharmaceutical intermediates, and cosmetic raw materials due to their excellent biological functions, and market demand continues to rise.
[0003] However, the efficient and green extraction process for wolfberry polysaccharides still faces many problems, one of which is insufficient yield. Insufficient polysaccharide yield directly leads to extremely low raw material utilization, with a large amount of effective components in wolfberry raw materials failing to be fully extracted. This results in a serious waste of high-quality wolfberry resources, significantly increases the raw material cost per unit product, and weakens the product's price competitiveness in the market.
[0004] Therefore, it is necessary to propose a green extraction method for high-yield wolfberry polysaccharides. Summary of the Invention
[0005] This invention proposes a green extraction method for wolfberry polysaccharides, which solves the problem of low polysaccharide extraction yield in related technologies.
[0006] The technical solution of this invention is as follows: This invention proposes a green extraction method for Lycium barbarum polysaccharides, comprising the following steps: S1. Dried goji berries are pulverized and sieved to obtain goji berry powder; S2. Add the wolfberry powder to water, soak it, add compound enzyme, and then enzymatically hydrolyze and inactivate the enzyme to obtain an enzymatic hydrolysate. S3. The enzymatic hydrolysate is concentrated to obtain a concentrated solution, activated carbon is added, and the solution is filtered and centrifuged to obtain a supernatant. S4. The supernatant is subjected to alcohol precipitation and centrifugation to obtain a precipitate, which is then washed and dried to obtain the wolfberry polysaccharide; The complex enzyme is composed of cellulase, pectinase and xylanase in a mass ratio of 1:2 to 2.2:1.
[0007] As a further technical solution, in step S2, the mass ratio of the goji berry powder to the water is 1:4~6, preferably 1:5, the soaking time is 8~10h, and the enzymatic hydrolysis is a two-stage enzymatic hydrolysis, with different temperatures and times for the first and second stages of enzymatic hydrolysis.
[0008] As a further technical solution, the temperature of the first enzymatic hydrolysis stage is 40~42℃ and the time is 30~40min, and the temperature of the second enzymatic hydrolysis stage is 45~48℃ and the time is 60~70min.
[0009] In the green extraction method of wolfberry polysaccharides of the present invention, a segmented enzymatic hydrolysis method is adopted to better match enzyme activity, maximize the cell wall breaking efficiency of the compound enzyme, and effectively improve the yield of wolfberry polysaccharides.
[0010] As a further technical solution, the amount of the compound enzyme added is 2% to 2.5% of the mass of the wolfberry powder.
[0011] As a further technical solution, the enzyme inactivation temperature is 90~100℃ and the time is 5~10min.
[0012] As a further technical solution, the addition of activated carbon includes the following steps: first, add wood-based powdered activated carbon, stir for 20-30 minutes, filter to obtain filtrate, add coconut shell activated carbon to the filtrate, and stir for 10-15 minutes.
[0013] As a further technical solution, the amount of wood-based powdered activated carbon added is 2% to 3% of the mass of the concentrate, the particle size of the wood-based powdered activated carbon is 200 to 325 mesh, the amount of coconut shell activated carbon added is 1% to 1.5% of the mass of the concentrate, and the particle size of the coconut shell activated carbon is 10 to 20 mesh.
[0014] In the green extraction method of wolfberry polysaccharides of this invention, 200-325 mesh wood-based powdered activated carbon is first added. It has a large specific surface area and is rich in polar functional groups on its surface, which can efficiently remove impurities such as soluble pigments, residual proteins, and small molecule organic acids. Then, 10-20 mesh coconut shell activated carbon is added, which can further adsorb trace colloidal impurities and pigments remaining in the filtrate. The process works synergistically to effectively improve the purity of wolfberry polysaccharides.
[0015] As a further technical solution, in step S3, the centrifugation time is 10~20 min and the centrifugation speed is 4000~6000 rpm.
[0016] As a further technical solution, in step S3, the concentrated solution is 1 / 3 of the volume of the enzymatic hydrolysate.
[0017] As a further technical solution, in step S4, the alcohol precipitation includes the following steps: concentrating the supernatant to a concentrated mixture with a concentration of 60wt%~65wt%, adding ethanol for precipitation for 10~12h, wherein the concentration of the ethanol is 95wt%, and the volume ratio of the ethanol to the concentrated mixture is 1:4~5.
[0018] As a further technical solution, in step S4, the solution used for washing is anhydrous ethanol, and the number of washing cycles is 2 to 4.
[0019] The working principle and beneficial effects of this invention are as follows: In this invention, a green extraction method for Lycium barbarum polysaccharides utilizes a complex enzyme composition consisting of cellulase, pectinase, and xylanase in a mass ratio of 1:2 to 2.2:1. This enzymatic hydrolysis extracts the polysaccharides, increasing their yield. The cell wall of Lycium barbarum is primarily composed of a dense structure formed by the interweaving of cellulose, pectin, and hemicellulose, encapsulating the internal polysaccharides. In this invention, pectinase is added to decompose the pectin components in the cell wall, breaking down the binding framework and creating a looser, more porous structure. Cellulase degrades the cellulose matrix within the cell wall, further disrupting its supporting framework. Xylanase decomposes the xylan in the hemicellulose, removing residual fibrous components from the cell wall. The synergistic effect of these three enzymes rapidly and effectively disrupts the Lycium barbarum cell wall structure, allowing for the full release of the encapsulated polysaccharides and significantly improving the polysaccharide yield. Detailed Implementation
[0020] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.
[0021] In the following examples and comparative examples: Wood-based powdered activated carbon: particle size 200 mesh; Coconut shell activated carbon: particle size 20 mesh; Cellulase: Enzyme activity is 10000 U / g; Pectinase: Enzyme activity is 100,000 U / g; Xylanase: Enzyme activity is 10000 U / g.
[0022] Example 1 A green extraction method for Lycium barbarum polysaccharides includes the following steps: S1. Dried goji berries are pulverized and passed through a 40-mesh sieve to obtain goji berry powder; S2. Add wolfberry powder to water, with a mass ratio of wolfberry powder to water of 1:5. After soaking for 8 hours, add compound enzyme, enzymatically hydrolyze at 40℃ for 40 minutes, then enzymatically hydrolyze at 45℃ for 70 minutes, and finally inactivate the enzyme at 90℃ for 10 minutes to obtain the enzymatic hydrolysate. S3. Concentrate the enzymatic hydrolysate to one-third of its original volume to obtain a concentrated solution. Add activated carbon, stir for 30 minutes, filter, and centrifuge at 4000 rpm for 20 minutes to obtain the supernatant. The activated carbon is wood-based powdered activated carbon, and the amount of activated carbon added is 3% of the mass of the concentrated solution. S4. The supernatant was concentrated to a concentration of 60 wt%, and 95 wt% ethanol was added to precipitate the mixture for 10 h. The volume ratio of ethanol to the concentrated mixture was 1:4. The mixture was centrifuged to obtain the precipitate. The precipitate was washed twice with anhydrous ethanol and freeze-dried to obtain wolfberry polysaccharide. The complex enzyme consists of cellulase, pectinase, and xylanase in a mass ratio of 1:2:1.
[0023] Example 2 A green extraction method for Lycium barbarum polysaccharides includes the following steps: S1. Dried goji berries are pulverized and passed through a 40-mesh sieve to obtain goji berry powder; S2. Add wolfberry powder to water, with a mass ratio of wolfberry powder to water of 1:5. After soaking for 9 hours, add compound enzyme, enzymatically hydrolyze at 40℃ for 40 minutes, then enzymatically hydrolyze at 45℃ for 70 minutes, and finally inactivate the enzyme at 90℃ for 10 minutes to obtain the enzymatic hydrolysate. S3. The enzymatic hydrolysate was concentrated to one-third of its original volume to obtain a concentrated solution. Activated carbon was added, and the solution was stirred for 40 minutes, then filtered. The solution was centrifuged at 5000 rpm for 15 minutes to obtain the supernatant. The activated carbon was wood-based powdered activated carbon, and the amount of activated carbon added was 4% of the mass of the concentrated solution. S4. The supernatant was concentrated to a concentration of 60 wt%, and 95 wt% ethanol was added to precipitate the mixture for 10 h. The volume ratio of ethanol to the concentrated mixture was 1:4. The mixture was centrifuged to obtain the precipitate. The precipitate was washed twice with anhydrous ethanol and freeze-dried to obtain wolfberry polysaccharide. The complex enzyme consists of cellulase, pectinase, and xylanase in a mass ratio of 1:2.1:1.
[0024] Example 3 A green extraction method for Lycium barbarum polysaccharides includes the following steps: S1. Dried goji berries are pulverized and passed through a 40-mesh sieve to obtain goji berry powder; S2. Add wolfberry powder to water, with a mass ratio of wolfberry powder to water of 1:5. After soaking for 10 hours, add compound enzyme, enzymatically hydrolyze at 42℃ for 30 minutes, then enzymatically hydrolyze at 48℃ for 60 minutes, and finally inactivate the enzyme at 100℃ for 5 minutes to obtain the enzymatic hydrolysate. S3. The enzymatic hydrolysate was concentrated to one-third of its original volume to obtain a concentrated solution. Activated carbon was added, and the mixture was stirred for 45 minutes, then filtered. The solution was centrifuged at 6000 rpm for 10 minutes to obtain the supernatant. The activated carbon was wood-based powder, and the amount of activated carbon added was 4.5% of the mass of the concentrated solution. S4. The supernatant was concentrated to a concentration of 65 wt%, and 95 wt% ethanol was added to precipitate the mixture for 12 h. The volume ratio of ethanol to the concentrated mixture was 1:5. The mixture was centrifuged to obtain the precipitate. The precipitate was washed three times with anhydrous ethanol and then freeze-dried to obtain wolfberry polysaccharide. The complex enzyme consists of cellulase, pectinase, and xylanase in a mass ratio of 1:2.2:1.
[0025] Example 4 Compared with Example 2, Example 4 differs in that, in this example, step S2 includes the following: adding wolfberry powder to water, with a mass ratio of wolfberry powder to water of 1:5, soaking for 9 hours, adding compound enzyme, enzymatic hydrolysis at 40°C for 110 minutes, and inactivating the enzyme at 90°C for 10 minutes to obtain enzymatic hydrolysate.
[0026] Example 5 The difference between Example 5 and Example 2 is that the activated carbon is coconut shell activated carbon.
[0027] Example 6 Compared with Example 2, Example 6 differs in that, in this example, step S3 includes the following: the enzymatic hydrolysate is concentrated to one-third of its original volume to obtain a concentrate, wood-based powdered activated carbon is added at a concentration of 2.5% of the concentrate mass, the mixture is stirred for 25 minutes, filtered to obtain a filtrate, coconut shell activated carbon is added to the filtrate at a concentration of 1.5% of the concentrate mass, the mixture is stirred for 10 minutes, filtered, and centrifuged at 5000 rpm for 15 minutes to obtain a supernatant.
[0028] Example 7 Compared with Example 2, Example 7 differs in that, in this example, step S3 includes the following: the enzymatic hydrolysate is concentrated to one-third of its original volume to obtain a concentrate, coconut shell activated carbon is added at a concentration of 1.5% of the concentrate mass, the mixture is stirred for 10 minutes, filtered to obtain a filtrate, wood powdered activated carbon is added to the filtrate at a concentration of 2.5% of the concentrate mass, the mixture is stirred for 25 minutes, filtered, and centrifuged at 5000 rpm for 15 minutes to obtain a supernatant.
[0029] Comparative Example 1 Compared with Example 2, the difference in Comparative Example 1 is that the complex enzyme is only pectinase.
[0030] Comparative Example 2 Compared with Example 2, Comparative Example 2 differs in that the complex enzyme consists of cellulase and pectinase in a mass ratio of 1:2.1.
[0031] Experimental Example 1 The Lycium barbarum polysaccharides obtained in Examples 1-4 and Comparative Examples 1-2 were weighed to obtain the yield of Lycium barbarum polysaccharides.
[0032] The test results are shown in Table 1: Table 1 Performance test results of Examples 1-4 and Comparative Examples 1-2
[0033] As shown in Table 1, when the complex enzyme is composed of cellulase, pectinase and xylanase, the yield of wolfberry polysaccharides can be increased.
[0034] Experimental Example 2 The purity of the Lycium barbarum polysaccharides obtained in Examples 2 and 5-7 was determined by the phenol-sulfuric acid method.
[0035] The test results are shown in Table 2: Table 2 Performance test results of Examples 2 and 5-7
[0036] As shown in Table 2, the purity of the wolfberry polysaccharide obtained by adding wood powdered activated carbon and coconut shell activated carbon to the concentrate in sequence is higher.
[0037] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A green extraction method for Lycium barbarum polysaccharides, characterized in that, Includes the following steps: S1. Dried goji berries are pulverized and sieved to obtain goji berry powder; S2. Add the wolfberry powder to water, soak it, add compound enzyme, and then enzymatically hydrolyze and inactivate the enzyme to obtain an enzymatic hydrolysate. S3. The enzymatic hydrolysate is concentrated to obtain a concentrated solution, activated carbon is added, and the solution is filtered and centrifuged to obtain a supernatant. S4. The supernatant is subjected to alcohol precipitation and centrifugation to obtain a precipitate, which is then washed and dried to obtain the wolfberry polysaccharide; The complex enzyme is composed of cellulase, pectinase and xylanase in a mass ratio of 1:2 to 2.2:
1.
2. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, In step S2, the mass ratio of the goji berry powder to the water is 1:4~6, the soaking time is 8~10h, and the enzymatic hydrolysis is a two-stage enzymatic hydrolysis, with different temperatures and times for the first and second stages.
3. The green extraction method for Lycium barbarum polysaccharides according to claim 2, characterized in that, The first enzymatic hydrolysis is performed at a temperature of 40-42℃ for 30-40 minutes, and the second enzymatic hydrolysis is performed at a temperature of 45-48℃ for 60-70 minutes.
4. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, The amount of the compound enzyme added is 2% to 2.5% of the mass of the wolfberry powder.
5. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, The enzyme inactivation temperature is 90~100℃, and the time is 5~10min.
6. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, The addition of activated carbon includes the following steps: first, add wood-based powdered activated carbon, stir for 20-30 minutes, filter to obtain filtrate, add coconut shell activated carbon to the filtrate, and stir for 10-15 minutes.
7. The green extraction method for Lycium barbarum polysaccharides according to claim 6, characterized in that, The amount of wood-based powdered activated carbon added is 2% to 3% of the mass of the concentrate, and the particle size of the wood-based powdered activated carbon is 200 to 325 mesh. The amount of coconut shell activated carbon added is 1% to 1.5% of the mass of the concentrate, and the particle size of the coconut shell activated carbon is 10 to 20 mesh.
8. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, In step S3, the centrifugation time is 10-20 minutes and the centrifugation speed is 4000-6000 rpm.
9. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, In step S4, the alcohol precipitation includes the following steps: concentrating the supernatant to a concentrated mixture with a concentration of 60wt%~65wt%, adding ethanol for precipitation for 10~12h, wherein the concentration of the ethanol is 95wt%, and the volume ratio of the ethanol to the concentrated mixture is 1:4~5.
10. The green extraction method for Lycium barbarum polysaccharides according to claim 1, characterized in that, In step S4, the washing solution used is anhydrous ethanol, and the washing is performed 2 to 4 times.