Liquid dressing for skin repair and preparation method and application thereof

Abstract

The invention belongs to the technical field of functional dressings, and discloses a liquid dressing for skin repair as well as a preparation method and application thereof, the liquid dressing for skin repair is prepared from the following raw materials in percentage by weight: 1-5% of zinc hyaluronate, 2-10% of giant salamander collagen, 1-8% of aloe polysaccharide, 0.5-6% of L-lactic acid, 0.5-2% of carbomer, 0.1-0.5% of preservative, 0.1-2% of pH regulator and the balance of purified water. The components of the dressing are reasonably proportioned and synergistically gained, and the dressing has the effects of resisting bacteria, resisting oxidation, promoting angiogenesis and the like, can effectively promote skin wound repair, and also has good safety and use comfort.

Description

A liquid dressing for skin repair, its preparation method and application Technical Field

[0001] This invention relates to the field of functional dressings, specifically to a liquid dressing for skin repair, its preparation method, and its application. Background Technology

[0002] The skin is the body's basic protective barrier against external aggressors. As understanding of skin care deepens, the demand for care for non-chronic wounds (such as superficial wounds, mechanical wounds, post-surgical suture wounds, and wounds after laser / chemical peels / minimally invasive cosmetic procedures) is increasing. Isolating the wound from the external environment with skin dressings provides a suitable moist environment for wound healing, prevents infection by pathogens, and enhances the skin's repair function, thus helping wounds heal quickly and reducing the likelihood of scarring.

[0003] Currently available skin repair dressings have relatively limited functions. While they can maintain skin moisture and provide some assistance in skin repair, most dressings are insufficient in combating bacterial infection and promoting skin repair. This can easily lead to prolonged wound healing periods, bacterial infection, increased inflammation, and severe scarring. Therefore, the development of effective medical dressings for non-chronic wound healing is an urgent market need. Summary of the Invention

[0004] (I) Technical Problems to be Solved In view of this, the present invention proposes a liquid dressing for skin repair, its preparation method and application. The liquid dressing is composed of active substances such as zinc hyaluronic acid, giant salamander collagen, aloe polysaccharide, and L-lactic acid in a reasonable ratio. It has significantly enhanced antibacterial infection and wound repair effects, high safety and comfortable skin feel.

[0005] (II) Technical Solution The technical solution of the present invention is as follows: In the first aspect, the present invention provides a liquid dressing for skin repair, which is made from the following raw materials in weight percentages: 1-5% zinc hyaluronic acid, 2-10% giant salamander collagen, 1-8% aloe polysaccharide, 0.5-6% L-lactic acid, 0.5-2% carbomer, 0.1-0.5% preservative, 0.1%-2% pH adjuster, and the balance being purified water.

[0006] Preferably, a liquid dressing for skin repair is made from the following raw materials in weight percentages: 1-3% zinc hyaluronic acid, 4-8% giant salamander collagen, 2-5% aloe polysaccharide, 0.5-2% L-lactic acid, 0.5-1% carbomer, 0.1-0.3% preservative, 0.5%-2% pH adjuster, and the balance being purified water.

[0007] A further preferred embodiment of a liquid dressing for skin repair is made from the following raw materials in weight percentages: 2% zinc hyaluronic acid, 6% giant salamander collagen, 3% aloe polysaccharide, 1% L-lactic acid, 1% carbomer, 0.2% preservative, 1% pH adjuster, and the balance being purified water.

[0008] Preferably, the molecular weight of the zinc hyaluronic acid is 800,000 to 1,200,000 Da.

[0009] Preferably, the molecular weight of the giant salamander collagen is 1000~2000 Da.

[0010] Preferably, the carbomer is carbomer 940.

[0011] Optionally, the preservative is a mixture of sodium ethylparaben and sodium propylparaben.

[0012] Preferably, the preservative is a 1:1 mixture of sodium ethylparaben and sodium propylparaben.

[0013] Preferably, the pH adjuster is sodium hydroxide.

[0014] Secondly, the present invention provides an application of a liquid dressing for skin repair or a liquid dressing for skin repair prepared by the aforementioned preparation method in the preparation of medical dressing products for skin wound repair.

[0015] Preferably, the medical dressing is a skin repair solution or a mask / patch.

[0016] More preferably, the medical dressing is used for the care of non-chronic wounds (such as superficial wounds, postoperative suture wounds, mechanical trauma wounds, and wounds after laser / chemical peels / minimally invasive cosmetic procedures).

[0017] Thirdly, this invention provides a method for preparing a liquid dressing for skin repair, comprising the following steps: S1: Weigh the prescribed amount of carbomer powder and add it to a portion of purified water, let it stand to fully absorb water and swell, until no white powder is visible on the surface and no powder clumps are visible at the bottom, then stir at low speed until homogeneous to obtain solution A; S2: Weigh the prescribed amounts of zinc hyaluronic acid, aloe polysaccharide, giant salamander collagen, L-lactic acid and preservative, add them to the remaining amount of purified water, and stir thoroughly at 30~60 ℃ until completely dissolved to obtain solution B; S3: Mix solution A and solution B, and stir thoroughly until homogeneous to obtain solution C; S4: Adjust the pH of solution C to 4.5~6.5 using a pH adjuster to obtain a viscous dressing solution; S5: After ultrasonically removing air bubbles from the obtained dressing solution, encapsulate it and sterilize it by CO60 irradiation, thus completing the process.

[0018] Preferably, in steps S1 to S4, the stirring speed is 3000 rpm.

[0019] Preferably, in step S4, the pH of the solution is adjusted to 5.0.

[0020] Preferably, the irradiation sterilization dose in step S5 is 30 kGy, and the sterilization time is 6 hours.

[0021] Preferably, in step S5, the packaging method is to seal the dressing solution in a glass bottle or to soak the dressing solution in non-woven fabric in the shape of a face mask, eye mask, patch, etc., and then seal it in an aluminum foil bag.

[0022] The components and their effects in this invention are as follows: Zinc is an essential trace element for the human body, possessing various physiological functions in the skin, including anti-inflammatory, antioxidant, antibacterial, wound-healing, and sebum-inhibiting properties. Zinc hyaluronic acid is a zinc salt of hyaluronic acid, combining the benefits of zinc ions with the moisturizing, viscoelastic, and lubricating properties of hyaluronic acid, without causing skin irritation or delayed-type hypersensitivity reactions, thus offering significant advantages in skin repair applications.

[0023] Giant salamander collagen is extracted from the skin of the giant salamander. It possesses bioactivities such as moisturizing, anti-inflammatory, antioxidant, and promoting skin collagen synthesis, making it highly valuable for promoting skin repair and maintaining skin health. Due to differences in amino acid composition and cross-linking degree, giant salamander collagen has advantages over collagen from livestock such as cattle, pigs, and sheep, including lower molecular weight, fewer biological contaminants, trace amounts of toxins, lower antigenicity, and lower allergenicity.

[0024] Aloe polysaccharides are the main bioactive components of aloe vera gel, possessing natural moisturizing properties as well as various physiological functions such as anti-inflammation, anti-oxidation, immune regulation, and wound healing promotion. Furthermore, aloe polysaccharides can inhibit the growth of bacteria and viruses, preventing post-traumatic infections.

[0025] L-lactic acid is an alpha-hydroxy acid that gently removes dead skin cells, promotes skin cell renewal, and stimulates collagen production. L-lactic acid also has anti-inflammatory properties, which can relieve skin redness and irritation.

[0026] Carbomer is a commonly used thickener that gives liquid dressings appropriate viscosity and provides good uniform dispersion and stability, thus facilitating application, reducing skin irritation, and enhancing the user experience and therapeutic effect of the product.

[0027] Changes in pH value can affect the viscosity and consistency of carbomer. Adjusting the system to the appropriate pH value using a pH adjuster can improve the stability of the product and optimize its physical properties, making it smoother and easier to apply.

[0028] Compared with the prior art, the beneficial effects of the present invention are: 1. The liquid dressing for skin repair provided by the present invention has a significantly enhanced wound repair effect by rationally combining active substances such as zinc hyaluronic acid, giant salamander collagen, aloe polysaccharide, and L-lactic acid.

[0029] 2. By optimizing the proportions of each component and the preparation process, the present invention produces a transparent, viscous gel-like dressing for skin repair. It has good fluidity, a delicate texture, moderate viscosity, and high comfort for use.

[0030] 3. The liquid dressing for skin repair provided by this invention is available in different packaging forms and is suitable for application on different parts of the body and in different scenarios.

[0031] 4. The giant salamander collagen added to the liquid dressing for skin repair provided by this invention has advantages such as lower molecular weight, fewer biological pollutants, trace amounts of toxins, low antigenicity, and low allergenicity compared to the collagen from cattle, pigs, sheep, and other livestock due to differences in amino acid composition and cross-linking degree. The prepared liquid dressing has high safety. Attached Figure Description

[0032] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0033] Figure 1 shows the antibacterial ability test results of the liquid dressing for skin repair.

[0034] Figure 2 shows the results of the antioxidant capacity test for the liquid dressing for skin repair.

[0035] Figure 3 shows the effect of liquid dressings for skin repair on promoting the migration of human umbilical vein endothelial cells (HUVECs). Detailed Implementation

[0036] The technical solution of this patent will be further described in detail below with reference to specific embodiments.

[0037] Unless otherwise specified, all reagents and materials described in the examples are commercially available.

[0038]

Example 1

[0039] Among them, zinc hyaluronic acid has a molecular weight of 800,000 to 1,200,000 Da; giant salamander collagen has a molecular weight of 1,000 to 2,000 Da; carbomer is carbomer 940; the preservative is a 1:1 mixture of sodium ethylparaben and sodium propylparaben; and the pH adjuster is sodium hydroxide.

[0040] [Example 2] A liquid dressing for skin repair is made from the following raw materials in weight percentages: 3% zinc hyaluronic acid, 8% giant salamander collagen, 2% aloe polysaccharide, 1% L-lactic acid, 1% carbomer, 0.2% preservative, 1% pH adjuster, and the balance being purified water.

[0041] Among them, zinc hyaluronic acid has a molecular weight of 800,000 to 1,200,000 Da; giant salamander collagen has a molecular weight of 1,000 to 2,000 Da; carbomer is carbomer 940; the preservative is a 1:1 mixture of sodium ethylparaben and sodium propylparaben; and the pH adjuster is sodium hydroxide.

[0042]

Example 3

[0043] Among them, zinc hyaluronic acid has a molecular weight of 800,000 to 1,200,000 Da; giant salamander collagen has a molecular weight of 1,000 to 2,000 Da; carbomer is carbomer 940; the preservative is a 1:1 mixture of sodium ethylparaben and sodium propylparaben; and the pH adjuster is sodium hydroxide.

[0044] The preparation methods for Examples 1-3 above are as follows: S1: Weigh the prescribed amount of carbomer powder and add it to a portion of purified water. Let it stand to fully absorb water and swell. When no white powder is visible on the surface and no powder clumps are visible at the bottom, stir at low speed until homogeneous to obtain solution A; S2: Weigh the prescribed amounts of zinc hyaluronic acid, aloe polysaccharide, giant salamander collagen, L-lactic acid, and preservative, and add them to the remaining purified water. Stir thoroughly at 30-60 °C until completely dissolved to obtain solution B; S3: Mix solution A and solution B and stir thoroughly until homogeneous to obtain solution C; S4: Adjust the pH of solution C to 5.0 using a pH adjuster to obtain a viscous dressing solution; S5: After ultrasonically removing air bubbles from the obtained dressing solution, seal it in a glass bottle and sterilize it by CO60 irradiation at a dose of 30 kGy for 6 hours.

[0045]

Comparative Example 1

[0046]

Comparative Example 2

[0047]

Comparative Example 3

[0048]

Comparative Example 4

[0049] [Test Example 1] Antibacterial properties of a liquid dressing for skin repair were tested. Specifically, the liquid dressing for skin repair was exposed to Escherichia coli (E. coli, 1×10⁻⁶) for 10 days. 6 CFU / mL) and Staphylococcus aureus (S. aureu, 1×10⁻⁶ ...). 6 The bacterial suspension (CFU / mL) was co-cultured at 37 °C for 6 h. The cultured suspension was diluted and evenly spread onto LB agar medium. After incubation at 37 °C for 24 h, the colony count on the medium was observed. The test results are shown in Figure 1. The skin repair liquid dressing prepared in Example 3 exhibits good antibacterial activity and can effectively inhibit the growth of Escherichia coli and Staphylococcus aureus.

[0050] [Test Example 2] The antioxidant properties of the skin repair liquid dressing were tested using a DPPH free radical scavenging kit. The test results are shown in Figure 2. The skin repair liquid dressing prepared in Example 3 effectively scavenged DPPH and exhibited good antioxidant capacity.

[0051] [Test Example 3] To investigate the effect of a skin repair liquid dressing on HUVEC migration. HUVECs (1×10⁻⁶) were... 4 Cells (number / mL) were seeded into 24-well culture plates. When the cells reached approximately 90% confluence, they were treated with a culture medium containing a skin-repairing liquid dressing. Cell migration was observed using an inverted microscope 24 hours later. The results are shown in Figure 2. The skin-repairing liquid dressing prepared in Example 3 significantly promoted HUVEC migration, indicating its skin-repairing effect.

[0052] [Test Example 4] To test the skin repair effect of a liquid dressing for skin repair. 160 patients who had just undergone microneedling surgery were randomly selected, and there were no significant differences in the basic information of the patients.

[0053] Treatment method: After cleansing the skin, apply a skin repair liquid dressing to the affected area and gently massage until absorbed. Use twice daily, morning and evening, for 10 days, and record the therapeutic effect. A commercially available product, "Yeast Recombinant Collagen Gel Dressing," was used as a positive control.

[0054] Efficacy assessment: A reduction of more than 90% in patients' erythema, edema, stinging pain, and pigmentation is considered a significant effect.

[0055] The condition is considered effective if the patient's erythema, edema, stinging, and pigmentation are reduced by more than 60%.

[0056] Skin feel rating: Users rate the comfort of using the liquid dressing on their skin. The maximum score is 10 points, with higher scores indicating greater comfort.

[0057] The results are shown in Table 1.

[0058] Table 1. Evaluation of the therapeutic effects of liquid dressings for skin repair

[0059] The test results above show that the liquid dressing for skin repair provided by this invention has antibacterial, antioxidant, and angiogenesis-promoting properties, effectively promoting skin repair, and offering a comfortable feel and high safety. Example 3 exhibits the best appearance and skin repair effect. Comparison of Examples 1-3 and Comparative Examples 1-4 demonstrates that optimizing the formulation of each component in the liquid dressing can improve the therapeutic effect and skin comfort of the hydrogel.

[0060] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A liquid dressing for skin repair, characterized in that, The dressing is made from the following raw materials in weight percentages: 1-5% zinc hyaluronic acid, 2-10% giant salamander collagen, 1-8% aloe polysaccharide, 0.5-6% L-lactic acid, 0.5-2% carbomer, 0.1-0.5% preservative, 0.1%-2% pH adjuster, and the balance being purified water.

2. The liquid dressing for skin repair according to claim 1, characterized in that, The dressing is made from the following raw materials in weight percentages: zinc hyaluronic acid 1-3%, giant salamander collagen 4-8%, aloe polysaccharide 2-5%, L-lactic acid 0.5-2%, carbomer 0.5-1%, preservative 0.1-0.3%, pH adjuster 0.5%-2%, and the balance being purified water.

3. The liquid dressing for skin repair according to claim 1, characterized in that, The dressing is made from the following ingredients in weight percentages: 2% zinc hyaluronic acid, 6% giant salamander collagen, 3% aloe polysaccharide, 1% L-lactic acid, 1% carbomer, 0.2% preservative, 1% pH adjuster, and the remainder is purified water.

4. The liquid dressing as described in any one of claims 1 to 3, characterized in that, The zinc hyaluronic acid has a molecular weight of 800,000 to 1,200,000 Da, the giant salamander collagen has a molecular weight of 1,000 to 2,000 Da, and the carbomer is carbomer 940.

5. The liquid dressing as described in any one of claims 1 to 3, characterized in that, The preservative is a 1:1 mixture of sodium ethylparaben and sodium propylparaben, and the pH adjuster is sodium hydroxide.

6. The use of the liquid dressing according to any one of claims 1 to 5 in the preparation of medical dressing products for skin wound repair.

7. The use of the liquid dressing according to any one of claims 1 to 5 in the preparation of skin repair solution or skin repair mask / patch.

8. The application of the liquid dressing according to any one of claims 1 to 5 in the care of non-chronic wounds (such as superficial wounds, postoperative sutured wounds, mechanical trauma wounds, and wounds after laser / chemical peels / micro-plastic surgery).

9. The method for preparing the liquid dressing according to any one of claims 1 to 5, characterized in that, The method is as follows: S1: Weigh the prescribed amount of carbomer powder and add it to part of purified water. Let it stand to fully absorb water and swell. When no white powder is visible on the surface and no powder clumps are visible at the bottom, stir at low speed until homogeneous to obtain solution A; S2: Weigh the prescribed amounts of zinc hyaluronic acid, aloe polysaccharide, giant salamander collagen, L-lactic acid, and preservative, and add them to the remaining amount of purified water. Stir thoroughly at 30-50 ℃ until completely dissolved to obtain solution B; S3: Mix solution A and solution B, and stir thoroughly until homogeneous to obtain solution C; S4: Adjust the pH of solution C to 4.5~6.5 using a pH adjuster to obtain a viscous dressing solution; S5: After ultrasonically removing air bubbles from the obtained dressing solution, seal it and sterilize it by CO60 irradiation to complete the process.

10. The method for preparing the liquid dressing according to claim 9, characterized in that, In steps S1 to S4, the stirring speed is 3000 rpm.

11. The method for preparing the liquid dressing according to claim 9, characterized in that, In step S4, the pH of the solution is adjusted to 5.

0.

12. The method for preparing the liquid dressing according to claim 9, characterized in that, In step S5, the irradiation sterilization dose is 30 kGy, and the sterilization time is 6 hours.

13. The method for preparing the liquid dressing according to claim 9, characterized in that, In step S5, the packaging method is to seal the dressing solution in a glass bottle or to soak the dressing solution in non-woven fabric in the shape of a face mask, eye mask, or patch and then seal it in an aluminum foil bag.

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