Antibacterial peptide crude liquid separation and purification device

Abstract

The invention relates to the technical field of biological medicine manufacturing, in particular to an antibacterial peptide crude liquid separation and purification device which comprises a storage barrel and a heat exchange mechanism installed on the storage barrel, an ultrafiltration mechanism is installed at the bottom of the storage barrel, a top cover is installed on the storage barrel, an extraction mechanism is installed on the top cover, and a feeding mechanism is arranged above the top cover. A pretreatment mechanism is mounted on the top cover; a pretreatment mechanism is mounted on the top cover, when an antibacterial peptide crude liquid is added into the storage barrel by the feeding mechanism, the antibacterial peptide crude liquid firstly passes through the interior of the filter tank, a filter tank and a cleaning tank are arranged in the filter tank, the antibacterial peptide crude liquid is conveyed into the filter tank, impurities in the antibacterial peptide crude liquid are pretreated by a filter plate, and the impurities in the antibacterial peptide crude liquid are cleaned by the cleaning tank; the blockage of the bottom of the storage barrel caused by coarse impurities is avoided, the blockage time of the polyethersulfone ultrafiltration membrane at the bottom of the storage barrel is prolonged, and the filtering effect is improved.

Description

A crude antimicrobial peptide separation and purification device Technical Field

[0001] This invention relates to the field of biopharmaceutical manufacturing technology, specifically to an antimicrobial peptide crude liquid separation and purification device. Background Technology

[0002] Antimicrobial peptides are a class of small molecule polypeptides widely found in organisms and are key components of the innate immune system. They possess advantages such as broad-spectrum antibacterial activity, low susceptibility to drug resistance, and good thermal stability, making them highly promising for applications in the biopharmaceutical manufacturing field. The separation and purification of crude antimicrobial peptides is a core step in obtaining high-purity, high-activity antimicrobial peptides in biopharmaceutical manufacturing. This requires considering the physicochemical properties of antimicrobial peptides, such as their cationicity, amphiphilicity, and molecular weight differences, and following the principle of "first crude enrichment, then fine purification, maintaining activity throughout the process." During the separation and purification of crude antimicrobial peptides, storage tanks are generally used for storage. The core logic of the separation and purification steps is "pretreatment for impurity removal → crude enrichment → fine purification → post-treatment identification." Filtration is generally performed using ultrafiltration membranes.

[0003] Currently, the separation and purification of crude antimicrobial peptides in biopharmaceutical manufacturing typically involves adding the crude liquid to a storage tank. A filter membrane is installed at the bottom of the tank to precipitate and filter impurities. The supernatant on top of the tank is drawn off by an extraction pipe, while the lower layer is filtered and discharged. The separation and purification device for crude antimicrobial peptides can be referenced from the existing patent publication number CN113527413B. However, this device only uses a filter membrane at the bottom of the storage tank to filter impurities. No pretreatment component for impurities is installed at the crude liquid inlet pipe. Therefore, after the crude liquid enters the filter membrane at the bottom of the storage tank, impurities are easily introduced into the feed. Accumulation at the pipe accelerates the clogging of the ultrafiltration membrane, reduces the discharge rate of coarse liquid, and affects the discharge of treated liquid. The ultrafiltration membrane, located at the bottom of the storage tank and horizontally installed on the feed pipe, continuously accumulates impurities on its surface as the filtration process progresses. Without disassembling the ultrafiltration membrane, it is difficult to quickly clean these impurities, leading to a significant decrease in the filtration efficiency of the ultrafiltration membrane. When treating the coarse antimicrobial peptide solution for precipitation, the supernatant needs to be extracted and separated. The extraction tube is fixed to the top of the storage tank with screws, making it difficult to raise and lower the tube and accurately adjust the extraction height according to the real-time liquid level of the supernatant, ultimately affecting the extraction accuracy of the supernatant. Summary of the Invention

[0004] To address the problems in the prior art, the present invention provides an apparatus for separating and purifying crude antimicrobial peptides.

[0005] The technical solution adopted by this invention to solve its technical problem is: an antimicrobial peptide crude liquid separation and purification device, comprising a storage tank and a heat exchange mechanism installed on the storage tank, an ultrafiltration mechanism installed at the bottom of the storage tank, a top cover installed on the storage tank, an extraction mechanism installed on the top cover, a feeding mechanism provided above the top cover, and a pretreatment mechanism installed on the top cover; the pretreatment mechanism includes a second support, a second support installed on the top cover, a filter tank installed on the second support, and the filter tank having a filter tank and a washing tank inside, the bottom of the washing tank... The filter tank is equipped with a drain outlet, a drain pipe is installed at the bottom of the drain outlet, a filter plate is rotatably connected to the filter tank, the filter plate extends into the interior of the filter tank, a gear is rotatably connected to the side wall of the filter plate, a slide bar is slidably connected to the side wall of the filter tank, a rack is installed on the slide bar, and there is a distance between the rack and the gear in the horizontal direction, a drive component is installed on the side wall of the filter tank, and the telescopic end of the drive component is fixedly connected to the slide bar, and an inlet pipe is installed at the bottom of the filter tank, the inlet pipe passes through the top cover and extends into the interior of the storage tank.

[0006] Specifically, the pretreatment mechanism also includes a support frame, which is installed between the bottom of the filter tank and the storage tank, and the support frame is inclined.

[0007] Specifically, the bottom of the filter tank and the bottom of the cleaning tank are both inclined in opposite directions, and the filter plate is arranged parallel to the bottom of the filter tank.

[0008] Specifically, there are two gears, which are symmetrically arranged about the center of the filter plate. Slide strips are slidably connected to both sides of the filter pool. The ends of the two slide strips are I-shaped. Driving components are installed on both sides of the filter pool. The telescopic ends of the two driving components are fixedly connected to the two slide strips respectively.

[0009] Specifically, a protective cover with an arc-shaped side structure is fixedly connected to the surface edge of the filter plate, and the opposite sides of the filter tank and the cleaning tank have an arc-shaped structure.

[0010] Specifically, the feeding mechanism includes a fixed frame, with the fixed frame fixedly connected between the two slide bars. A conveying pipe is installed on the fixed frame, extending above the filter plate. The distance between the conveying pipe and the right side of the filter tank is less than the distance between the gear and the rack.

[0011] Specifically, the ultrafiltration mechanism includes a feed pipe, a feed pipe installed at the bottom of the storage tank, a filter box installed at the bottom of the feed pipe, a discharge pipe connected to the bottom of the filter box, a fixing frame inserted obliquely on the filter box, a polyethersulfone ultrafiltration membrane installed on the fixing frame, and a sealing gasket that abuts against the fixing frame being fixedly connected to the side wall of the filter box by screws.

[0012] Specifically, the ultrafiltration mechanism also includes an upper valve and a lower valve. The upper valve is installed on the feed pipe, and the lower valve is installed on the discharge pipe. An inlet pipe is installed at an angle on the side wall of the filter box. The inlet pipe is located at the bottom of the polyethersulfone ultrafiltration membrane and is equipped with a control valve. A backflush pipe is installed at an angle on the side wall of the feed pipe. A sealing plug is threaded onto the backflush pipe. The backflush pipe is located above the polyethersulfone ultrafiltration membrane, and the angle of the backflush pipe is opposite to that of the inlet pipe.

[0013] Specifically, the extraction mechanism includes an extraction tube, which is slidably connected to the top cover and extends into the storage tank. A flexible tube is connected to the extraction tube, and a mounting plate is fixedly connected to the bottom of the extraction tube. Multiple filter membranes are provided at the bottom of the mounting plate. A fixing rod is inserted into the filter membranes and the mounting plate. A nut that abuts against the filter membrane is threaded onto the fixing rod. Two mounting slots are opened opposite each other on the extraction tube, and a crossbeam is fixedly connected to each of the two mounting slots by screws. The fixing rod passes through the crossbeam, and a nut located at the top of the crossbeam is threaded onto the fixing rod. Two drive blocks are symmetrically slidably connected to the top cover about the extraction tube. Insert plates are fixedly connected to the opposite sides of the two drive blocks, and the insert plates are slidably connected to the top cover. Slide rods are fixedly connected to the back sides of the two drive blocks, and springs are provided on the two slide rods. Multiple slots are axially oppositely opened on the sidewall of the extraction tube, and the insert plates are inserted into the slots.

[0014] Specifically, the heat exchange mechanism includes a jacket, the side wall of the storage tank is provided with a jacket, and heat exchange tubes are installed inside the jacket.

[0015] The beneficial effects of this invention are: 1. The antimicrobial peptide crude liquid separation and purification device of this invention has a pretreatment mechanism installed on the top cover. When the feeding mechanism adds the antimicrobial peptide crude liquid into the storage tank, the antimicrobial peptide crude liquid first passes through the inside of the filter tank. The filter tank is equipped with a filter tank and a washing tank. The antimicrobial peptide crude liquid is transported to the inside of the filter tank, where impurities in the antimicrobial peptide crude liquid are pretreated by the filter plate, avoiding blockage at the bottom of the storage tank by coarser impurities, extending the clogging time of the polyethersulfone ultrafiltration membrane at the bottom of the storage tank, and improving the filtration effect. When there are many impurities on the surface of the filter plate, it can... Activate the two drive units, and their telescopic ends extend to push the slide bar. At this time, the conveying pipe moves out from above the filter tank. As the slide bar moves, the two racks mesh with the two gears, and the racks drive the gears to rotate counterclockwise. This further rotates the filter plate from the filter tank into the cleaning tank, which is filled with clean water. The drive units reciprocate to move the filter plate up and down, facilitating the cleaning of the filter plate with clean water and the filtration of impurities in the coarse liquid. After cleaning, the slide bar is retracted and reset by the drive units, and the conveying pipe returns to the top of the filter tank, and the filter plate returns to the inside of the filter tank.

[0016] 2. The antimicrobial peptide crude liquid separation and purification device of the present invention has an ultrafiltration mechanism installed at the bottom of the storage tank. After the antimicrobial peptide crude liquid settles inside the storage tank, larger impurities are adsorbed and filtered by the polyethersulfone ultrafiltration membrane inside the filter box. The processed antimicrobial peptide crude liquid is discharged from the discharge pipe to the next processing stage. When the polyethersulfone ultrafiltration membrane inside the fixed frame needs to be cleaned, the upper valve on the feed pipe and the lower valve on the discharge pipe can be closed, and the control valve on the water inlet pipe can be opened. Clean water is flushed into the interior of the filter box from the water inlet pipe and rinsed from the bottom to the top of the polyethersulfone ultrafiltration membrane. The sealing plug on the return pipe is opened to flush out the cleaned impurities without disassembling the fixed frame for cleaning, thereby improving the filtration effect of the polyethersulfone ultrafiltration membrane on the antimicrobial peptide crude liquid.

[0017] 3. The antimicrobial peptide crude liquid separation and purification device of the present invention has an extraction mechanism installed on the top cover. When extracting the supernatant inside the storage tank, two driving blocks can slide in opposite directions to allow two insert plates to slide out from the slots on the side wall of the extraction tube. At this time, the extraction tube can be raised and lowered on the top cover. After adjusting the extraction tube to a suitable position, the two driving blocks are released, and the two insert plates are reset by springs. The two insert plates are inserted into the corresponding slots on the extraction tube, realizing the adjustment and fixation of the extraction tube, which is conducive to the precise extraction of the supernatant according to the stratification position of the supernatant. Attached Figure Description

[0018] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0019] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a schematic diagram of the connection structure of the storage tank, inlet pipe and jacket of the present invention; Figure 3 is a schematic diagram of the connection structure of the filter tank, filter tank and cleaning tank of the present invention; Figure 4 is a schematic diagram of the connection structure of the gear, slide bar and rack of the present invention; Figure 5 is a schematic diagram of the connection structure of the slide bar, fixing frame and filter plate of the present invention; Figure 6 is a schematic diagram of the connection structure of the feed pipe, filter box and water inlet pipe of the present invention; Figure 7 is a schematic diagram of the connection structure of the filter box, fixing frame and polyethersulfone ultrafiltration membrane of the present invention; Figure 8 is a schematic diagram of the connection structure of the filter box, water inlet pipe and back flushing pipe of the present invention; Figure 9 is a schematic diagram of the connection structure of the extraction pipe, hose and crossbeam of the present invention; Figure 10 is an enlarged schematic diagram of the structure of part A shown in Figure 9; Figure 11 is a schematic diagram of the connection structure of the extraction pipe, fixing rod and mounting plate of the present invention.

[0020] In the diagram: 1. Storage tank; 2. Pretreatment mechanism; 201. Support 1; 202. Filter tank; 203. Inlet pipe; 204. Filter tank; 205. Filter plate; 206. Gear; 207. Cleaning tank; 208. Drain outlet; 209. Drain pipe; 210. Support 2; 211. Protective cover; 212. Rack; 213. Drive component; 214. Sliding bar; 3. Heat exchange mechanism; 301. Heat exchange tube; 302. Jacket; 4. Top cover; 5. Ultrafiltration mechanism; 501. Feed pipe; 502. Filter box; 503. Discharge pipe; 504. 505. Sealing gasket; 506. Upper valve; 507. Lower valve; 508. Backflush pipe; 509. Sealing plug; 510. Inlet pipe; 511. Control valve; 512. Fixing frame; 513. Polyethersulfone ultrafiltration membrane; 6. Extraction mechanism; 601. Extraction pipe; 602. Hoses; 603. Crossbeam; 604. Fixing rod; 605. Drive block; 606. Insert plate; 607. Slot; 608. Slide rod; 609. Spring; 610. Mounting groove; 611. Mounting plate; 612. Filter membrane; 7. Feeding mechanism; 701. Fixing frame; 702. Conveying pipe. Detailed Implementation

[0021] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0022] As shown in Figures 1-11, the present invention provides an antimicrobial peptide crude liquid separation and purification device, comprising a storage tank 1 and a heat exchange mechanism 3 installed on the storage tank 1. An ultrafiltration mechanism 5 is installed at the bottom of the storage tank 1. A top cover 4 is installed on the storage tank 1, and an extraction mechanism 6 is installed on the top cover 4. A feeding mechanism 7 is located above the top cover 4, and a pretreatment mechanism 2 is installed on the top cover 4. The pretreatment mechanism 2 includes a second support 210, which is installed on the top cover 4. A filter tank 202 is installed on the second support 210. The filter tank 202 contains a filter tank 204 and a washing tank 207. A drain outlet 208 is opened at the bottom of the washing tank 207. A drain pipe 209 is installed at the bottom of the filter tank 202. A filter plate 205 is rotatably connected to the filter tank 202, extending into the interior of the filter trough 204. A gear 206 is rotatably connected to the side wall of the filter plate 205. A slide bar 214 is slidably connected to the side wall of the filter tank 202, and a rack 212 is installed on the slide bar 214. There is a horizontal distance between the rack 212 and the gear 206. A drive component 213 is installed on the side wall of the filter tank 202, and the telescopic end of the drive component 213 is fixedly connected to the slide bar 214. An inlet pipe 203 is installed at the bottom of the filter trough 204, penetrating the top cover 4 and extending into the interior of the storage tank 1. The pretreatment mechanism 2 also includes a support bracket 201, which is installed between the bottom of the filter tank 202 and the storage tank 1. The support bracket 201 is inclined. The bottom of the filter tank 204 and the bottom of the cleaning tank 207 are both inclined away from each other. The filter plate 205 is parallel to the bottom of the filter tank 204. There are two gears 206, which are symmetrically arranged about the center of the filter plate 205. Sliding strips 214 are slidably connected to both sides of the filter tank 202. The ends of the two sliding strips 214 are I-shaped. Driving components 213 are installed on both sides of the filter tank 202. The telescopic ends of the two driving components 213 are respectively connected to the two sliding strips 214. The filter plate 205 is fixedly connected to the filter tank 204 and the cleaning tank 207. The filter tank 204 and the cleaning tank 207 are fixedly connected to the filter tank 205. The feed mechanism 7 includes a fixed frame 701. The fixed frame 701 is fixedly connected between the two slide bars 214. The fixed frame 702 is installed on the fixed frame 701. The feed pipe 702 extends to the top of the filter plate 205. The distance between the feed pipe 702 and the right side of the filter tank 202 is less than the distance between the gear 206 and the rack 212. The heat exchange mechanism 3 includes a jacket 302. The side wall of the storage tank 1 is provided with a jacket 302. The heat exchange pipe 301 is installed inside the jacket 302.The delivery pipe 702 is connected to the pipeline for conveying the crude liquid via a flexible connecting pipe. Antimicrobial peptide crude liquid is added to the storage tank 1 through the delivery pipe 702. Before entering the storage tank 1, the crude antimicrobial peptide liquid undergoes preliminary filtration by the filter plate 205 inside the filter tank 202, pre-treating impurities in the crude antimicrobial peptide liquid. Impurities in the crude liquid are intercepted by the filter plate 205, achieving preliminary pre-treatment. This step effectively prevents coarser impurities from entering the bottom of the storage tank 1, preventing blockage in subsequent processes, extending the service life of the polyethersulfone ultrafiltration membrane 512 at the bottom of the storage tank 1, and improving the overall filtration effect. After pre-treatment, the impurities in the crude antimicrobial peptide liquid are discharged from the filter tank 204 into the inlet pipe 203, and finally flow from the inlet pipe 203 into the storage tank 1. When cleaning the filter plate 205 is required, both drives are activated simultaneously. Part 213, the driving part 213 is a cylinder. The telescopic end of the driving part 213 extends, pushing the slide bar 214 to move. At this time, the conveying pipe 702 moves out from above the filter tank 204. As the slide bar 214 moves, the rack 212 meshes with the gear 206, driving the gear 206 to rotate counterclockwise, thereby causing the filter plate 205 to rotate from the filter tank 204 into the cleaning tank 207. The cleaning tank 207 is filled with clean water. The driving part 213 reciprocates, driving the filter plate 205 to move up and down, so that the clean water can fully rinse the filter plate 205 and remove surface impurities. After cleaning, the driving part 213 retracts and resets, the slide bar 214 returns to its initial position, the conveying pipe 702 returns to above the filter tank 204, and the filter plate 205 also returns to the filter tank 204 to continue the filtration work. After preliminary treatment, the crude antimicrobial peptide solution enters the storage tank 1 for sedimentation and ultrafiltration treatment.

[0023] Specifically, referring to Figures 1, 2, 6, 7, and 8, the ultrafiltration mechanism 5 includes a feed pipe 501, which is installed at the bottom of the storage tank 1. A filter box 502 is installed at the bottom of the feed pipe 501, and a discharge pipe 503 is connected to the bottom of the filter box 502. A fixing frame 511 is obliquely inserted into the filter box 502, and a polyethersulfone ultrafiltration membrane 512 is installed on the fixing frame 511. A sealing gasket 504 that abuts against the fixing frame 511 is fixedly connected to the side wall of the filter box 502 by screws. The ultrafiltration mechanism 5 also includes an upper valve 505 and a lower valve. 506. An upper valve 505 is installed on the feed pipe 501, and a lower valve 506 is installed on the discharge pipe 503. An inlet pipe 509 is installed at an angle on the side wall of the filter box 502. The inlet pipe 509 is located at the bottom of the polyethersulfone ultrafiltration membrane 512, and a control valve 510 is installed on the inlet pipe 509. A backflush pipe 507 is installed at an angle on the side wall of the feed pipe 501. A sealing plug 508 is threaded onto the backflush pipe 507. The backflush pipe 507 is located above the polyethersulfone ultrafiltration membrane 512, and the inclination direction of the backflush pipe 507 is opposite to that of the inlet pipe 509. (The last sentence appears to be incomplete and possibly refers to a pre-treated section.) After the crude antimicrobial peptide solution enters storage tank 1, it undergoes sedimentation. During this process, larger particles of impurities gradually settle to the bottom of storage tank 1. After sedimentation for a period of time, the upper valve 505 on the feed pipe 501 is opened, and the crude solution at the bottom of storage tank 1 flows into the filter box 502. The polyethersulfone ultrafiltration membrane 512 in the filter box 502 adsorbs and filters the crude solution, intercepting larger impurities on the surface of the polyethersulfone ultrafiltration membrane 512. The treated crude antimicrobial peptide solution is discharged from the discharge pipe 503 and enters the next processing stage. When the polyethersulfone ultrafiltration membrane 512 in the fixed frame 511... When cleaning is required, close the upper valve 505 on the feed pipe 501 and the lower valve 506 on the discharge pipe 503, and open the control valve 510 on the water inlet pipe 509. Clean water is flushed into the filter box 502 from the water inlet pipe 509 and rinsed upward from the bottom of the polyethersulfone ultrafiltration membrane 512 to remove impurities from the surface of the polyethersulfone ultrafiltration membrane 512. At the same time, open the sealing plug 508 on the return pipe 507 so that the flushed impurities are discharged from the return pipe 507 with the water flow. The cleaning can be completed without disassembling the fixing frame 511, which effectively improves the filtration effect of the polyethersulfone ultrafiltration membrane 512.

[0024] Specifically, referring to Figures 1, 2, 9, 10, and 11, the extraction mechanism 6 includes an extraction tube 601. An extraction tube 601 extending into the storage tank 1 is slidably connected to the top cover 4. A flexible tube 602 is connected to the extraction tube 601. A mounting plate 611 is fixedly connected to the inner bottom of the extraction tube 601. Multiple filter membranes 612 are provided at the bottom of the mounting plate 611. Fixing rods 604 are inserted into the filter membranes 612 and the mounting plate 611. Nuts that abut against the filter membranes 612 are threaded onto the fixing rods 604. The extraction tube 601 has opposite openings... The device has two mounting slots 610, each with a crossbeam 603 fixedly connected to it by screws. A fixing rod 604 passes through the crossbeam 603, and a nut is threaded onto the fixing rod 604 at the top of the crossbeam 603. Two drive blocks 605 are symmetrically slidably connected to the top cover 4 about the extraction tube 601. Insert plates 606 are fixedly connected to opposite sides of the two drive blocks 605, and are slidably connected to the top cover 4. Slide rods 608 are fixedly connected to the back of each drive block 605, and each slide rod 608 is equipped with a spring 609. The extraction tube 601 has multiple slots 607 axially oppositely formed on its side wall, and insert plates 606 are inserted into the slots 607. During the sedimentation of the antimicrobial peptide crude liquid, the liquid in the storage tank 1 will separate into layers, with the upper layer being the supernatant. When it is necessary to extract the supernatant, two driving blocks 605 on the opposite sliding top cover 4 are used to slide the two insert plates 606 out of the slots 607 on the side wall of the extraction tube 601. At this time, the extraction tube 601 can be raised and lowered on the top cover 4. According to the separation position of the supernatant, the extraction tube 601 can be adjusted to a suitable height. After completion, release the two drive blocks 605, and the two insert plates 606 will reset under the action of the spring 609 and be inserted into the corresponding slots 607 on the extraction tube 601 to fix the extraction tube 601. Finally, connect the relevant equipment through the extraction tube 601 to accurately extract the supernatant according to the stratification position. During the extraction process, impurities in the supernatant are filtered and intercepted by the filter membrane 612 to ensure the purity of the supernatant. When replacing the filter membrane 612, remove the crossbeam 603 to take out the fixing rod 604 and replace the filter membrane 612.

[0025] In use, this invention first connects the delivery pipe 702 to the pipeline for delivering the crude liquid via a flexible connecting pipe. The crude antimicrobial peptide liquid is then added to the storage tank 1 through the delivery pipe 702. Before entering the storage tank 1, the crude antimicrobial peptide liquid undergoes preliminary filtration by the filter plate 205 inside the filter tank 202, pre-treating impurities in the liquid. The impurities are intercepted by the filter plate 205, achieving preliminary pre-treatment. This step effectively prevents coarser impurities from entering the bottom of the storage tank 1, preventing blockage in subsequent processes, extending the service life of the polyethersulfone ultrafiltration membrane 512 at the bottom of the storage tank 1, and improving the overall filtration effect. After pre-treatment, the impurities in the crude antimicrobial peptide liquid are discharged from the filter tank 204 into the inlet. Inside the liquid pipe 203, the liquid eventually flows from the inlet pipe 203 into the storage tank 1. When the filter plate 205 needs to be cleaned, two drive components 213 are activated simultaneously. The drive components 213 are cylinders. The telescopic ends of the drive components 213 extend, pushing the slide bar 214 to move. At this time, the delivery pipe 702 moves out from above the filter tank 204. As the slide bar 214 moves, the rack 212 meshes with the gear 206, driving the gear 206 to rotate counterclockwise. This causes the filter plate 205 to rotate from the filter tank 204 into the cleaning tank 207. The cleaning tank 207 is filled with clean water. The drive components 213 reciprocate, moving the filter plate 205 up and down, allowing the clean water to thoroughly rinse the filter plate 205 and remove surface impurities. After cleaning is completed... The drive unit 213 retracts and resets, the slide bar 214 returns to its initial position, the conveying pipe 702 returns to above the filter tank 204, and the filter plate 205 also returns to the filter tank 204 to continue the filtration process. After preliminary treatment, the crude antimicrobial peptide solution enters the storage tank 1 for sedimentation and ultrafiltration. Then, after the pretreated crude antimicrobial peptide solution enters the storage tank 1, it undergoes sedimentation. During this process, larger particles of impurities gradually settle to the bottom of the storage tank 1. After sedimentation for a period of time, the upper valve 505 on the feed pipe 501 is opened, and the crude solution at the bottom of the storage tank 1 flows into the filter box 502. The polyethersulfone ultrafiltration membrane 512 in the filter box 502 adsorbs and filters the crude solution, intercepting larger impurities in the polyethersulfone ultrafiltration membrane. On the surface of the filter membrane 512, the treated crude antimicrobial peptide solution is discharged from the discharge pipe 503 and enters the next processing stage. When the polyethersulfone ultrafiltration membrane 512 in the fixed frame 511 needs to be cleaned, the upper valve 505 on the feed pipe 501 and the lower valve 506 on the discharge pipe 503 are closed, and the control valve 510 on the water inlet pipe 509 is opened. Clean water is flushed into the filter box 502 from the water inlet pipe 509 and rinsed from the bottom of the polyethersulfone ultrafiltration membrane 512 upwards to remove impurities from the surface of the polyethersulfone ultrafiltration membrane 512. At the same time, the sealing plug 508 on the return pipe 507 is opened so that the flushed impurities are discharged from the return pipe 507 with the water flow. The cleaning can be completed without disassembling the fixed frame 511, which effectively improves the filtration effect of the polyethersulfone ultrafiltration membrane 512.Finally, during the sedimentation of the crude antimicrobial peptide solution, the liquid in storage tank 1 will exhibit stratification, with the upper layer being the supernatant. When it is necessary to extract the supernatant, the two driving blocks 605 on the opposing sliding top cover 4 will slide the two insert plates 606 out of the slots 607 on the side wall of the extraction tube 601. At this time, the extraction tube 601 can be raised and lowered on the top cover 4. According to the stratification position of the supernatant, the extraction tube 601 is adjusted to a suitable height. After adjustment, the two driving blocks 605 are released, and the two insert plates 606... 06 is reset by the action of spring 609 and inserted into the corresponding slot 607 on the extraction tube 601 to fix the extraction tube 601. Finally, by connecting the extraction tube 601 to relevant equipment, the supernatant can be accurately extracted according to its stratification position. During the extraction process, impurities in the supernatant are filtered and intercepted by the filter membrane 612 to ensure the purity of the supernatant. When replacing the filter membrane 612, the crossbeam 603 can be removed to take out the fixing rod 604 and replace the filter membrane 612.

[0026] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0027] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A device for separating and purifying crude antimicrobial peptides, characterized in that: The system includes a storage tank (1) and a heat exchange mechanism (3) installed on the storage tank (1). An ultrafiltration mechanism (5) is installed at the bottom of the storage tank (1). A top cover (4) is installed on the storage tank (1). An extraction mechanism (6) is installed on the top cover (4). A feeding mechanism (7) is provided above the top cover (4). A pretreatment mechanism (2) is installed on the top cover (4). The pretreatment mechanism (2) includes a second support (210). The second support (210) is installed on the top cover (4). A filter tank (202) is installed on the second support (210). The filter tank (202) has a filter tank (204) and a cleaning tank (207) inside. A drain outlet (208) is opened at the bottom of the cleaning tank (207). A drain pipe (209) is installed at the bottom of the drain outlet (208). A filter plate (205) is rotatably connected to the filter pool (202), the filter plate (205) extends into the interior of the filter tank (204), a gear (206) is rotatably connected to the side wall of the filter plate (205), a slide bar (214) is slidably connected to the side wall of the filter pool (202), a rack (212) is installed on the slide bar (214), the rack (212) and the gear (206) are at a distance in the horizontal direction, a drive component (213) is installed on the side wall of the filter pool (202), the telescopic end of the drive component (213) is fixedly connected to the slide bar (214), an inlet pipe (203) is installed at the bottom of the filter tank (204), the inlet pipe (203) penetrates the top cover (4), and the inlet pipe (203) extends into the interior of the storage tank (1).

2. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: The pretreatment mechanism (2) also includes a support (201), which is installed between the bottom of the filter tank (202) and the storage tank (1). The support (201) is inclined.

3. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: The bottom of the filter tank (204) and the bottom of the cleaning tank (207) are both inclined in opposite directions, and the filter plate (205) is parallel to the bottom of the filter tank (204).

4. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: Two gears (206) are provided, and the two gears (206) are symmetrically arranged about the center of the filter plate (205). Sliding strips (214) are slidably connected to both sides of the filter pool (202). The ends of the two sliding strips (214) are I-shaped. Driving components (213) are installed on both sides of the filter pool (202). The telescopic ends of the two driving components (213) are fixedly connected to the two sliding strips (214) respectively.

5. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: A protective cover (211) with an arc-shaped side is fixedly connected to the surface edge of the filter plate (205), and the opposite sides of the filter tank (204) and the cleaning tank (207) have an arc-shaped structure.

6. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: The feeding mechanism (7) includes a fixed frame (701), which is fixedly connected between the two slide bars (214). A conveying pipe (702) is installed on the fixed frame (701). The conveying pipe (702) extends above the filter plate (205). The distance between the conveying pipe (702) and the right side of the filter tank (202) is less than the distance between the gear (206) and the rack (212).

7. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: The ultrafiltration mechanism (5) includes a feed pipe (501), the bottom of the storage tank (1) is equipped with a feed pipe (501), the bottom of the feed pipe (501) is equipped with a filter box (502), the bottom of the filter box (502) is connected to a discharge pipe (503), a fixing frame (511) is inserted obliquely on the filter box (502), a polyethersulfone ultrafiltration membrane (512) is installed on the fixing frame (511), and a sealing gasket (504) that abuts against the fixing frame (511) is fixedly connected to the side wall of the filter box (502) by screws.

8. The antimicrobial peptide crude liquid separation and purification device according to claim 7, characterized in that: The ultrafiltration mechanism (5) also includes an upper valve (505) and a lower valve (506). The upper valve (505) is installed on the feed pipe (501), and the lower valve (506) is installed on the discharge pipe (503). The side wall of the filter box (502) is inclinedly installed with a water inlet pipe (509). The water inlet pipe (509) is located at the bottom of the polyethersulfone ultrafiltration membrane (512), and a control valve (510) is installed on the water inlet pipe (509). The side wall of the feed pipe (501) is inclinedly installed with a backflush pipe (507). A sealing plug (508) is threaded onto the backflush pipe (507). The backflush pipe (507) is located above the polyethersulfone ultrafiltration membrane (512), and the inclination direction of the backflush pipe (507) is opposite to that of the water inlet pipe (509).

9. The antimicrobial peptide crude liquid separation and purification device according to claim 8, characterized in that: The extraction mechanism (6) includes an extraction tube (601). The top cover (4) is slidably connected to the extraction tube (601) extending into the storage tank (1). A flexible tube (602) is connected to the extraction tube (601). An installation plate (611) is fixedly connected to the bottom of the extraction tube (601). A plurality of filter membranes (612) are provided at the bottom of the installation plate (611). A fixing rod (604) is inserted into the filter membrane (612) and the installation plate (611). A nut that abuts against the filter membrane (612) is threaded onto the fixing rod (604). Two mounting slots (610) are opened opposite each other on the extraction tube (601). A crossbeam (604) is fixedly connected to each of the two mounting slots (610) by screws. 03), the fixing rod (604) passes through the crossbeam (603), and the fixing rod (604) is threaded with a nut located at the top of the crossbeam (603). The top cover (4) has two driving blocks (605) symmetrically slidably connected about the extraction tube (601). The two driving blocks (605) are fixedly connected to the opposite sides of each other with insert plates (606). The insert plates (606) are slidably connected to the top cover (4). The back sides of the two driving blocks (605) are fixedly connected with slide rods (608). The two slide rods (608) are provided with springs (609). The side wall of the extraction tube (601) is axially opened with multiple slots (607), and the insert plates (606) are inserted into the slots (607).

10. The antimicrobial peptide crude liquid separation and purification device according to claim 1, characterized in that: The heat exchange mechanism (3) includes a jacket (302), and the side wall of the storage tank (1) is provided with a jacket (302). A heat exchange tube (301) is installed inside the jacket (302).

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