Application of arabidopsis thaliana AtRLP30 gene in enhancing fungus resistance of plants

Abstract

The invention belongs to the technical field of plant genetic engineering, and relates to application of an arabidopsis thaliana-derived pattern recognition receptor AtRLP30 gene in improving the fungal disease resistance of plants. The nucleotide sequence of the AtRLP30 gene is as shown in SEQ ID NO. 1. By constructing a plant expression vector containing AtRLP30 and performing overexpression in the plant, the resistance of the plant to fungal pathogen infection can be remarkably improved. Preferably, the fungi comprise ascospora poplar and ascopolyspora poplar, and the fungi comprise ascospora poplar. Experimental results show that after the AtRLP30 is overexpressed in the poplar, the transgenic plant shows stronger active oxygen accumulation, MAPK signal activation and defense related gene expression after being infected by pathogenic bacteria, and the biomass and scab area of the pathogenic bacteria are remarkably reduced. The invention proves that the AtRLP30 has the function of enhancing the fungal disease resistance in the woody plant, and provides a new gene resource and technical approach for improving the forest disease resistance by utilizing a pattern recognition receptor.

Description

Technical Field

[0001] This invention belongs to the field of plant genetic engineering technology, specifically relating to Arabidopsis thaliana. AtRLP30 Application of genes in enhancing plant resistance to fungi. Background Technology

[0002] Perennial woody plants, such as poplars, are fast-growing species that not only provide timber, pulp, and carbon sequestration but also play a vital ecological role in landscaping. However, large-scale monoculture can easily lead to the spread of foliar diseases. Therefore, the biological stress faced by woody plants is multi-dimensional. Their perennial nature means that species like poplars face continuous pressure from accumulated pathogens throughout their life cycle, and monoculture not only creates ideal conditions for pathogen transmission but also weakens the system's natural buffering capacity by reducing stand biodiversity. The plant's innate immune system primarily relies on two types of immune receptors—pattern recognition receptors and nucleotide-binding leucine-rich repeat proteins—to recognize pathogen-related molecular patterns and effector proteins. However, this defense mechanism is strictly limited by the species' inherent disease-resistant gene pool. When encountering rapidly evolving new strains of pathogens, the fixed genetic background often exhibits significant defense blind spots, causing traditionally bred disease-resistant varieties to lose their advantage within a few years. This dynamic game is even more severe in the context of climate change. Changes in environmental factors such as temperature and humidity may disrupt the original host-pathogen interaction balance, causing latent diseases to break out into epidemics.

[0003] In summary, plants are constantly exposed to a variety of microbial pathogens and rely mainly on innate immunity for protection, which is severely limited by the species' own genetic background. Summary of the Invention

[0004] This invention aims to provide Arabidopsis thaliana. AtRLP30 The application of genes in enhancing plant resistance to fungi aims to address the technical problem that the resistance of perennial woody plant leaves to disease is limited by the species itself in existing technologies.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: overexpression AtRLP30 The application of gene-based biological products in plant antifungal infection control, the aforementioned AtRLP30 The nucleotide sequence of the gene is shown in SEQ ID NO.1.

[0006] Furthermore, the fungus includes *Dispora yangpanensis* (…). Marssonina brunnea ) and Populus polyps ( Septotis populiperda ).

[0007] Furthermore, the biological product includes: a recombinant plasmid or recombinant engineered bacteria containing the gene shown in SEQ ID NO.1.

[0008] Furthermore, the recombinant plasmid comprises pBI121-35S:RLP30:GFP, which is constructed according to the following method: AtRLP30 The gene was cloned into the modified dual plasmid vector pBI121-GFP containing a Gateway selection cassette and a C-terminal GFP tag.

[0009] Furthermore, the recombinant engineered bacteria are obtained by introducing recombinant plasmids into the engineered bacteria.

[0010] Furthermore, the host bacteria of the recombinant engineered bacteria include Agrobacterium GV3101.

[0011] Furthermore, overexpression AtRLP30 The method of using gene-based biological products to resist fungal infections in poplar trees is to inoculate plant explants with recombinant engineered bacteria.

[0012] The steps for infecting plant explants with the recombinant engineered bacteria are as follows: The recombinant engineered bacteria are cultured to obtain an inoculum solution; the inoculum solution is then transformed into plant explants; and through explant callus culture and induction of redifferentiation, overexpression is obtained. AtRLP30 Gene-based plants, achieving [something] in plants AtRLP30 Gene overexpression.

[0013] Furthermore, the plants include poplar and tobacco.

[0014] Furthermore, the poplar species mentioned include "Shanxin Poplar".

[0015] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention provides AtRLP30 Application of genes in resistance to plant fungal infections, overexpression AtRLP30 Genes enhance plant resistance to fungal infections, including *Dispora yangpanensis* (…). Marssonina brunnea ) and Populus polyps ( Septotis populiperda Overexpression AtRLP30 Genes enable plants to provide immune protection against fungal infections, not limited to the species' own genetic background. This study demonstrates the functional feasibility of Arabidopsis-derived immune receptors in woody plants and, for the first time, validated Arabidopsis-derived pattern recognition receptors in poplar. AtRLP30 The disease resistance function of plants provides experimental evidence for cross-species utilization of plant immune receptors.

[0016] (2) The invention relates to the following: AtRLP30 The gene significantly enhances plant resistance to fungal pathogens in resistance to plant fungal infections, and its expression... AtRLP30The transgenic plants infected with *Dispora populina* and *Polysporium populina* exhibited stronger reactive oxygen species accumulation, more significant MAPK signaling activation, and higher levels of defense gene expression. Simultaneously, pathogen biomass and lesion area were significantly reduced, indicating that... AtRLP30 The expression of this substance can significantly enhance the plant's resistance to fungal diseases.

[0017] (3) This invention uses reverse transcription PCR technology to clone from Arabidopsis thaliana AtRLP30 This invention confirms the role of genes in plants, highlighting them as important genes involved in plant disease resistance; it contributes to understanding... AtRLP30 The mechanism of gene action AtRLP30 The cloning of the gene lays the foundation for further understanding the genetic regulation of antibacterial activity in poplar and the disease resistance signal transduction pathway, which has important theoretical and practical significance. It provides new gene resources for disease-resistant breeding of forest trees, and shows that immune receptor genes derived from model plants can play a role in forest plants, providing a new molecular breeding strategy for breeding disease-resistant forest tree species.

[0018] (4) This invention provides heterologous expression AtRLP30 Proof of concept that can enhance the disease resistance of forest tree species; expression AtRLP30 The transgenic poplar lines exhibited a stronger immune response and less severe disease after infection with two major foliar fungal pathogens; confirming... AtRLP30 It is an important genetic resource for improving the disease resistance of woody perennial plants.

[0019] (5) The present invention provides AtRLP30 The pattern recognition receptor transfer functioned effectively as a heterologous immune receptor in poplar trees, conferring stronger and potentially more durable resistance to fungal pathogens; this indicates that pattern recognition receptor transfer is an effective strategy for constructing broad-spectrum immunity in forest trees. Attached Figure Description

[0020] Figure 1 For the construction and validation of transgenic plants; where A is a schematic diagram of the T-DNA region in the pBI121-35S:RLP30:GFP vector used for plant transformation; B is the use of AtRLP30 Representative PCR analysis of genomic DNA from transgenic poplar lines and wild-type plants using specific primers; WT represents wild-type; L1-L8 represent transgenic lines; C represents the expression of AtRLP30-GFP in the leaves of the transgenic 'Shanxin Yang' poplar, detected using anti-GFP antibody. The Coomassie brilliant blue stained gel below the immunoblot shows the representative loading amount for each sample; D represents... AtRLP30 Comparison of plant growth and morphological characteristics between transgenic and non-transgenic wild-type poplar seedlings; L1, L2 and L3 represent transgenic lines.

[0021] Figure 2In the diagram, A represents the observation of inoculation using DAB staining. MBMo The accumulation of reactive oxygen species in the leaves of *Populus shanxinensis*; B shows the observation of inoculated plants using DAB staining. SP The accumulation of reactive oxygen species in the leaves of *Populus shanxinensis*; leaves stained with DAB 24 h after inoculation; brown sediment indicates H2O2 accumulation; WT represents wild type; L1-L3 represent transgenic lines; C represents inoculation detected using phosphorylated p44 / 42 MAPK antibody. MBMo The activation status of MAPK in leaves of 1-month-old 'Shanxin Poplar' seedlings after transplantation; D represents the detection of inoculation using phosphorylated p44 / 42 MAPK antibody. SP The activation of MAPK in leaves of 1-month-old 'Shanxin Poplar' was assessed. Leaves were collected at specified time points for immunoblotting analysis. The Coomassie Brilliant Blue CBB staining gel below shows the representative loading amount of each sample. WT, wild type; L1, transgenic line.

[0022] Figure 3 for AtRLP30 Overexpression of defense-related genes PaPR1 The impact of expression; MBMo On days 0 and 1 post-infection, the leaves of *Populus spp.* PaPR1 The relative expression levels were shown in the bar chart using Mean ± SD. Data were from three independent experiments, and statistically significant differences were found between the means calculated using one-way ANOVA and Tukey's test for different letter representations, p < 0.05.

[0023] Figure 4 In the diagram, A represents infection. MBMo A) is a typical image of the leaves on day 8; B) is the result of real-time quantitative PCR assay in transgenic Populus tomentosa lines L1-L3 and wild-type Populus tomentosa WT. MBMo The relative biomass of fungal growth; C represents the infection rate. S. populiperda Typical image of the leaf on day 3; D is SP Analysis of lesion area in transgenic poplar and wild-type poplar after infection; statistical differences between the two types were determined using Student's t-test, **p<0.01, ***p<0.001; bar charts represent mean ± SD, and data were obtained from three independent experiments. Detailed Implementation

[0024] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art; the experimental methods used are all conventional methods; and the materials, reagents, etc. used are all commercially available.

[0025] Pathogen-associated molecular pattern triggering of immunity is abbreviated as PTI.

[0026] AtRLP30 The nucleotide sequence of the gene is shown below: Yangpandispora ( Marssonina brunnea The abbreviation for ) is MBMo Poplar leaves are covered with polysporum ( Septitis in poplar The abbreviation for ) is SP Or use S. populiperda express; MbMoEF1-α upstream primer: 5'-TCACCGTGATTTCATCAAGAAC-3', SEQ ID NO.2; MbMoEF1-α downstream primer: 5'-GGCAACAGTCTTTGGGTTGTAT-3', SEQ ID NO.3; PaPR1 upstream primer: 5'-TCAATGCCCACAATAATGCTCG-3', SEQ ID NO.4; PaPR1 downstream primer: 5'-TAAGATCACCACTACCTCCTGC-3', SEQ ID NO.5; PaEF1-α upstream primer: 5'-CCTGGACATCGTGACTTTATCA-3', SEQ ID NO.6; PaEF1-α downstream primer: 5'-GTCCATCTTGTTACAGCAGCAG-3', SEQ ID NO.7.

[0027] Example 1: Preparation of transgenic poplar lines The specific steps for preparing transgenic poplar varieties are as follows: S1, Hybrid poplar 'Shanxin Poplar' P. davidiana × P.bolleana Tissue culture was performed on half-concentration Murashige Skoog medium supplemented with 0.2 mg / L indole-3-butyric acid. The cultures were maintained at 23°C with a photoperiod of 16 h light / 8 h dark. Four-week-old rooted seedlings were transplanted into pots containing nutrient soil and grown under controlled greenhouse conditions at 23°C with a photoperiod of 16 h light / 8 h dark. Four weeks after transplanting, the fifth or sixth fully expanded leaf located below the stem tip was obtained from each seedling.

[0028] S2, MBMo and SP Two fungal strains were cultured on potato dextrose agar plates at 25°C. MBMo The method for preparing the conidial suspension is as follows: after rinsing the 10-day-old culture with sterile water, adjust the concentration to 1×10⁻⁶. 5 Conidia / mL. For SPFor the strain, a 5 mm diameter mycelial block was cut from the edge of a PDA culture that was in an active growth phase.

[0029] S3 AtRLP30 The coding sequence CDS for AT3G05360 was amplified from cDNA of Arabidopsis thaliana Columbia ecotype (SEQ ID NO.1) and cloned between the XbaI and PstI restriction sites of the modified dual plasmid vector pBI121-GFP containing a Gateway selection cassette and a C-terminal GFP tag, i.e., pBI121-35S:RLP30:GFP. The constructed recombinant plasmid was then transformed into Agrobacterium GV3101 strain via electroporation. The specific vector construction process is as follows:

[0030] RLP30 CDS with the stop codon removed was amplified using Arabidopsis cDNA as a template. The PCR product was then ligated with pENTR / D-linker-6 containing linker and GFP tags via double digestion with XbaI and PstI, respectively, to obtain the entry clone pENTR / D-linker-6::linker-RLP30-GFP. After sequencing verification, the target fragment was transformed into the 35S promoter-driven pBI121 expression vector using Gateway LR recombination, constructing pBI121-35S:RLP30:GFP.

[0031] S4. A 1 cm long leaf disc was cut from a 4-week-old 'Shanxin Poplar' seedling and inoculated with Agrobacterium for 30 min. After co-culturing for 2 days, the explants were transferred to rooting medium. MS medium was supplemented with 0.1 mg / L naphthaleneacetic acid, 0.2 mg / L 6-benzyladenine, 0.01 mg / L thiabendazole and 50 mg / L kanamycin. When the kanamycin-resistant seedlings reached about 1 cm in length, they were transferred to rooting medium. MS medium was supplemented with 0.2 mg / L IBA and 20 mg / L kanamycin to obtain the transgenic poplar line L1.

[0032] Experiment 1: Verification of Transgenic Plants Genomic DNA was extracted from leaf tissues of kanamycin-resistant and wild-type strains using a plant DNA extraction kit from Tiangen Biochemical. DNA quality and concentration were assessed by agarose gel electrophoresis, and quantification was performed using a NanoDrop ND-1000 spectrophotometer manufactured by NanoDrop Technologies, Wilmington, USA. KOD-plus-Neo DNA polymerase from Toyohiro Pharmaceuticals, Osaka, Japan, was used in conjunction with… AtRLP30PCR amplification was performed using specific primers. The PCR products were separated and purified by agarose gel electrophoresis and ligated into the pEASY-Blunt Zero cloning vector from Beijing Tiangen Biotech. The recombinant plasmid was transformed into *E. coli*, and positive clones were confirmed by Sanger sequencing to verify sequence identity. Total soluble protein was extracted from uniform 1 cm leaf discs. Total protein was separated on 12% SDS-PAGE and electrophoretically transferred to a Hybond ECL membrane. The membrane was incubated with rabbit polyclonal anti-GFP primary antibody, followed by incubation with alkaline phosphatase-labeled goat anti-rabbit IgG secondary antibody. Immunoblotting was performed using a 5-bromo-4-chloro-3-indolyl phosphate / p-nitroblue tetrazolium chloride substrate solution.

[0033] For evaluation AtRLP30 The gene was introduced into the hybrid poplar 'Shanxin Poplar' to investigate its function in the poplar's immune system. It was amplified from Arabidopsis cDNA. AtRLP30 The full-length coding sequence SEQ ID NO.1 was cloned into the binary vector pBI121 controlled by the CaMV 35S promoter. Figure 1 As shown in Figure A, the construct was introduced into *Populus shanxinense* using Agrobacterium GV3101-mediated leaf disc transformation. Genomic PCR using gene-specific primers revealed the amplification of the expected 450 bp fragment in the transgenic plants, while it was not detected in the wild-type plants, confirming the... AtRLP30 Successful integration, such as Figure 1 As shown in B in the figure. Immunoblot analysis using an anti-green fluorescent protein (GFP) antibody further validated protein accumulation, detecting a band of approximately 140 kDa corresponding to AtRLP30-GFP. Insertion of the same gene into different sites on the chromosome yielded eight independent transgenic lines. Signals were detected in all eight independent transgenic lines, while no related signals were observed in the wild-type plants, such as... Figure 1 As shown in C. Three representative strains, L1, L2, and L3, were randomly selected for subsequent experiments. Compared to the wild type, AtRLP30 Constitutive expression of genes did not cause detectable changes in plant growth or morphology, such as Figure 1 As shown in D in the diagram.

[0034] Experiment 2 H2O2 Detection H2O2 accumulation was detected using the DAB staining method. Leaves inoculated with fungal pathogens were immersed in 100 µg / mL DAB solution and incubated overnight in the dark. After staining, the leaves were boiled in 95% ethanol to decolorize, then mounted and photographed. The brown precipitate indicated H2O2 accumulation.

[0035] ROS accumulation was observed 24 hours post-inoculation using 3,3'-diaminobenzidine staining. Figure 2 As shown in A, with vaccination MBMoCompared to wild-type leaves, the transgenic lines exhibited significantly stronger brown coloration, indicating increased accumulation of hydrogen peroxide (H₂O₂). Figure 2 As shown in B, with vaccination SP Compared to wild-type leaves, the transgenic lines showed significantly stronger brown coloration, indicating increased accumulation of hydrogen peroxide (H2O2).

[0036] Experiment 3 Detection of MAPK phosphorylation To assess MAPK activation following pathogen inoculation, leaf tissues were collected at specified time points—0 h, 12 h, 24 h, and 36 h—and immediately frozen in liquid nitrogen. Immunoblot analysis was performed as described above, and activated MAPKs were detected using an anti-phosphorylated p44 / 42 MAP kinase antibody. Coomassie staining of the gels confirmed equal protein loading amounts.

[0037] For evaluation AtRLP30 To investigate whether expression affects PTI signal transduction in the "Shanxin Yang" variety, two hallmark immune responses following fungal pathogen inoculation were detected: reactive oxygen species (ROS) generation and MAPK activation.

[0038] Immunoblot analysis further showed that, Figure 2 As shown in C, MBMo Following infection, both wild-type and transgenic plants triggered MAPK phosphorylation. MBMo After infection, the activation levels of MAPK are similar across different genotypes; and SP Infection causes expression AtRLP30 The plants produced a significantly stronger MAPK response, such as Figure 2 As shown in D in the diagram. This enhanced signal transduction may reflect a more robust pathogen-associated molecular pattern, PAMP-induced plant disease resistance immune PTI mechanism, or... SP The involvement of additional receptors during infection. Taken together, these results indicate... AtRLP30 Overexpression of [the substance] can enhance the PTI-related response of poplar trees during fungal infection.

[0039] Experiment 4 AtRLP30 Promote early defense gene activation PaPR1 The measurement of expression involved spraying the transplanted 'Shanxin Poplar' seedlings. MBMo spore suspension 1×10 5Spores / mL were collected and covered with a glass beaker to maintain humidity. Leaf samples were then collected at 0 and 1 days post-infection, and total RNA was extracted using the RNAprep Pure Plant Kit. cDNA was then synthesized using the SuperScript III first-strand synthesis system. Internal control gene primers (SEQ ID NO. 6 and SEQ ID NO. 7) were designed based on the EF1-α sequence of Populus tomentosa. RT-qPCR was performed using a ViiATM 7 real-time quantitative PCR system with a reaction volume of 20 μL, using FastStart universal SYBR Green premixed buffer. Relative expression levels were determined by 2... -ΔΔCT The statistical analysis was performed using a one-way ANOVA on the RT-qPCR data from three independent experiments, followed by the Tukey test. PaPR1 The primer sequences for the gene are SEQ ID NO.4 and SEQ ID NO.5.

[0040] The systemic acquired resistance marker genes in wild-type and transgenic plants were analyzed using RT-qPCR technology. PaPR1 Transcriptional levels. Leaf samples were collected on day 0 and day 1 after infection with *Dioscorea opposita*. Figure 3 As shown, although PaPR1 In wild type and AtRLP30 Expression was induced in all transgenic plants on day 1 post-infection, but the transcriptional level in transgenic plants was significantly higher than that in wild-type plants. These results indicate that... AtRLP30 By enhancing the activation of early defense genes, the transgenic poplar's resistance to *Pseudomonas spp.* infection was improved.

[0041] Experiment 5 In transgenic plants AtRLP30 Overexpression of [specific ingredient] can enhance resistance to fungal pathogens. Will AtRLP30 Transgenic plants and transgenic lines L1, L2, and L3 bacterial seedlings with 10 μL MBMo Conidial suspension, 1×10 5 one conidia / mL or SP 5mm mycelial blocks were inoculated, and the inoculated leaves were placed in 9cm petri dishes containing 1.5% water agar and cultured at 25℃ with a photoperiod of 14h. MBMo Lesion formation of the strain was recorded on day 8 post-infection. SP The strain appeared on day 3 post-infection. Genomic DNA was extracted from inoculated leaves using the DNAsecure Plant DNA Extraction Kit. MBMo strains EF1-α Gene-designed qPCR-specific primers, SEQ ID NO.2 and SEQ ID NO.3, were used to design primers for the poplar tree 'Shanxin Yang'. EF1-α As an internal reference, 2-ΔΔCT Quantitative analysis of relative fungal biomass. Calculated using ImageJ software. SP The leaf area of ​​transgenic and wild-type poplars after infection was compared. Each experimental group contained at least 6 leaves for each genotype, and the experiment was repeated three times independently. Statistical analysis was performed using t-tests.

[0042] Pathogen infection experiments were conducted by inoculating detached leaves from transgenic plants L1, L2, and L3, and wild-type plants, respectively. MBMo Spores, 1×10 5 Conidia / mL, such as Figure 4 As shown, the results indicate that compared to the wild-type control, expression... AtRLP30 The plants infected with the fungus developed smaller necrotic lesions. This was confirmed by quantitative PCR. MBMo elongation factor EF1-α Genetic studies revealed that the fungal biomass of transgenic plants was significantly lower than that of wild-type plants. Quantitative studies based on image analysis further confirmed this. SP The lesion area in the transgenic leaves after infection. These results combined indicate that... AtRLP30 Overexpression of the drug significantly enhanced the resistance of hybrid poplar to two different semi-nutritive leaf pathogens.

[0043] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. Overexpression AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The AtRLP30 The nucleotide sequence of the gene is shown in SEQ ID NO.

1.

2. The overexpression according to claim 1 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The fungi include *Dispora yangpanensis* (… Marssonina brunnea ) and Populus polyps ( Septotis populiperda At least one of the following.

3. The overexpression according to claim 1 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The biological product includes: a recombinant plasmid or recombinant engineered bacteria containing the gene shown in SEQ ID NO.

1.

4. The overexpression according to claim 3 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The recombinant plasmid comprises pBI121-35S:RLP30:GFP, which is constructed according to the following method: Will AtRLP30 The gene was cloned into the modified dual plasmid vector pBI121-GFP containing a Gateway selection cassette and a C-terminal GFP tag.

5. The overexpression according to claim 3 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The recombinant engineered bacteria were obtained by introducing recombinant plasmids into the engineered bacteria.

6. The overexpression according to claim 5 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The host bacteria of the recombinant engineered bacteria include Agrobacterium GV3101.

7. The overexpression according to claim 5 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, overexpression AtRLP30 The method of using gene-based biological products to resist fungal infections in poplar trees is to inoculate plant explants with recombinant engineered bacteria.

8. The overexpression according to claim 7 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The steps for infecting plant explants with the recombinant engineered bacteria are as follows: The recombinant engineered bacteria were cultured to obtain an inoculum solution, which was then transformed into plant explants. Through explant callus culture and induced redifferentiation, overexpression was obtained. AtRLP30 Gene-based plants, achieving [something] in plants AtRLP30 Gene overexpression.

9. The overexpression according to claim 1 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The plants mentioned include poplar and tobacco.

10. The overexpression according to claim 9 AtRLP30 The application of gene-based biological products in plant antifungal infection control is characterized by, The poplar varieties mentioned include "Shanxin Poplar".

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