Microscopic imaging system with bright field imaging and phase difference imaging

Abstract

The invention relates to the technical field of microscopic detection, in particular to a microscopic imaging system with bright field imaging and phase difference imaging, which comprises an imaging module, a bright field imaging illumination module, a focusing module, a phase difference imaging illumination light source module and a control module. The problems that in the prior art, the switching time is long, the definition degree of images is prone to being affected in the switching process, the target form of bright field image and phase difference image shooting is changed, and result contrastive analysis is not facilitated are solved.

Description

A microscopic imaging system with bright-field imaging and phase-contrast imaging Technical Field

[0001] This invention relates to the field of microscopic detection technology, specifically to a microscopic imaging system with bright-field imaging and phase-contrast imaging. Background Technology

[0002] In the fields of clinical laboratory testing and environmental science, cell analysis and microbial analysis are core components of detection, and microscopy, as a fundamental technology in these fields, has been widely applied in scenarios such as blood cell classification, identification of formed elements in body fluids, and microbial morphology identification. Among these, cell analysis of body fluid samples (especially urine) is particularly crucial for the diagnosis of urinary system diseases—components in urine such as renal tubular epithelial cells, hyaline casts, and atypical red blood cells are directly related to the early diagnosis of diseases such as kidney damage, nephritis, and urinary tract stones, and the detection of these components is highly dependent on the clarity and accuracy of microscopic imaging technology.

[0003] However, the detection of low-contrast cells (such as unstained hyaline casts and erythrocytes) remains a technical challenge in cell analysis. With traditional bright-field imaging, the change in light intensity as it passes through the cell and matrix is ​​minimal, resulting in blurred cell edges and indistinguishable internal structures. In contrast, phase-contrast imaging technology significantly improves the detail resolution of low-contrast cells by converting the "optical path difference" between the cell and matrix into "brightness-darkness contrast." Phase-contrast imaging also significantly improves the accuracy of identifying hyaline casts and erythrocytes, making it the preferred technique for low-contrast cell detection.

[0004] In the field of environmental science, it is necessary to deeply analyze the microbial communities in environmental samples such as water and soil. The application of phase contrast microscopy in this field can help researchers observe the morphology and activity of microorganisms such as bacteria and algae in water samples, providing effective data support for research on environmental pollution and ecological balance. It can also support scientists in exploring the impact of different pollutants on microbial communities, thereby achieving a scientific assessment of environmental quality.

[0005] The core principle of traditional phase contrast microscopy is based on the "interference effect of light". Its key structure includes a condenser with a phase contrast ring and an objective with a phase plate. When a parallel light source passes through the phase ring of the condenser, two beams of light are formed. One beam is the "direct light" that does not pass through the sample (the optical path does not change), and the other beam is the "diffracted light" that passes through the sample. The two beams of light converge at the phase plate of the objective. The phase plate delays the phase of the direct light that does not pass through the sample, causing the direct light to interfere with the diffracted light that passes through the transparent sample. This transforms the optical path difference, which is originally invisible to the naked eye, into a contrasting image of light and dark.

[0006] Existing automated microscope technology solutions employ a single-optical-path design, relying on mechanical position switching to switch between bright-field and phase-contrast imaging. This results in inefficient function switching, long switching times, and the potential movement of cells, microorganisms, or bacteria during the switching process. This can affect image clarity and cause changes in the morphology of the target captured in bright-field and phase-contrast images, hindering comparative analysis of the results. Summary of the Invention

[0007] The purpose of this invention is to provide a microscopic imaging system with bright-field imaging and phase-contrast imaging to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a microscopic imaging system with bright-field imaging and phase-contrast imaging, comprising an imaging module, a bright-field imaging illumination module, a focusing module, a phase-contrast imaging illumination source module, and a control module. The bright-field imaging illumination and phase-contrast imaging illumination sources are converged into an optical path by a beam splitter and provide illumination to the imaging module. A stage is provided between the bright-field imaging illumination module and the imaging module. The imaging module includes a phase-contrast objective lens, a lens barrel is provided below the phase-contrast objective lens, and an image receiving device is provided below the lens barrel. The bright-field imaging illumination module includes an LED light source, a condenser lens, a field aperture, an aperture stop, and a condenser lens arranged sequentially along the optical path direction, and the above components together form a Köhler illumination system. The focusing module includes a stepper motor, a connecting plate and an optical coupler baffle are mounted on the outer wall of the stepper motor, and are also mounted on the connecting plate. When the moving parts move, an optical coupler is provided below the optical coupler baffle.

[0009] As a preferred embodiment of the present invention, the phase contrast imaging illumination source module adopts an LED array light source. The LED array adopted by the phase contrast imaging illumination source module is a ring LED structure composed of single LEDs, or an LED array of arbitrary shape composed of multiple LEDs and the LED array is lit in a ring state. Alternatively, the LED array adopted by the phase contrast imaging illumination source 40 is a single LED ring light source formed by multiple LED light-emitting chips arranged and packaged in a ring, or a single LED light source formed by multiple LED light-emitting chips arranged and packaged in an arbitrary shape and the LED light source is lit in a ring state. The LED light sources of both the bright field imaging illumination module and the phase contrast imaging illumination source module are white LED light sources.

[0010] As a preferred embodiment of the present invention, the transmittance of the beam splitter 6 is greater than the reflectance, and the control module controls the distance between the phase contrast objective and the target to be photographed, and takes a series of sample images at a fixed distance before and after the focal point of the phase contrast objective for focus finding and image synthesis. The number of images taken is greater than or equal to 1 and less than or equal to 20.

[0011] As a preferred embodiment of the present invention, the control module controls the distance between the phase contrast objective lens and the target to automatically perform focusing and image capture, and controls the illumination field imaging illumination module, the phase contrast imaging illumination source module and the imaging part to continuously capture bright field images and phase contrast images, or automatically determines and executes whether to capture phase contrast images based on input conditions.

[0012] As a preferred embodiment of the present invention, the input conditions include the ability to determine whether to capture a phase difference image based on a bright field image, the ability to determine whether to capture a phase difference image based on other unit test results, and the ability to select whether to capture a phase difference image in the software.

[0013] As a preferred embodiment of the present invention, the phase contrast objective can be an infinity conjugate objective or a finite conjugate objective. For an infinity conjugate objective, a tube lens needs to be set in the lens barrel to cooperate with imaging. For a finite conjugate objective, a lens does not need to be set in the lens barrel. The magnification of the sample by the imaging module is consistent with the magnification marked on the objective. The camera is a color camera.

[0014] In a preferred embodiment of the present invention, the stepper motor is connected to the component to be moved via a connecting plate. An optical coupler baffle is also mounted on the connecting plate. When the moving component moves, the optical coupler baffle blocks the optical coupler. Using the optical coupler position as the starting point, the distance between the phase contrast objective and the target is controlled. The control module controls the focusing and imaging process. Before capturing an image of each sample, the focus needs to be found. Starting from the optical coupler position, the stepper motor moves a fixed distance, bringing the phase contrast objective to a specific distance below the sample's focal point, and image capture begins. Each step of the stepper motor captures one image until it has moved past the specific distance to the focal point, at which point capturing stops. The captured images will include blurry images before reaching the focal point, gradually clearer images as it approaches the focal point, clear images upon reaching the focal point, and gradually blurry images moving away from the specific focal point. All images are saved for focal point analysis. When capturing a sample, based on the focal point position determined by the focusing analysis, the stepper motor moves the objective to a distance of 10 steps from the focal point, and image capture begins until it has moved past the focal point by 10 steps, at which point capturing stops, and the captured images are saved and analyzed. From the saved images, the algorithm analyzes and selects the clearest image, classifying and counting the targets in that image. More preferably, image fusion is performed on a series of saved images to widen the depth of field range of the objective lens, which can better restore cells whose volume exceeds the depth of field of the objective lens and cells located at different levels, thereby reducing missed detections and misclassifications.

[0015] In a preferred embodiment of the present invention, the control module controls the capture of bright-field images and phase-contrast images. Depending on actual needs, the control module can choose to capture only bright-field images. If there is doubt about the captured bright-field image and a phase-contrast image is required for review, the control module can choose to capture only the phase-contrast image. When both bright-field and phase-contrast images are needed, the control module can switch between capturing bright-field and phase-contrast images, i.e., capturing one bright-field image and then one phase-contrast image of the same target. The control module can automatically determine whether to capture a phase-contrast image in conjunction with other modules. During sample testing, the control module first captures a bright-field image, and then intelligently determines whether to capture a phase-contrast image based on the bright-field image results.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. In the present invention, by synchronously acquiring bright-field images and phase-contrast images at the same sampling position, dual information of bright-field images and phase-contrast images can be obtained, providing richer discrimination criteria for image processing algorithms, and ultimately achieving significant optimization of the reliability and accuracy of cell and microbial identification and classification. It is especially suitable for the detection needs of low-contrast cells in samples such as urine and water microorganisms. It adopts an architecture design of "dual illumination optical path + single imaging module". The imaging end is only equipped with one set of phase-contrast objective lens and camera. The switching between the two imaging modes of bright-field and phase-contrast is achieved by controlling the on and off of the light sources of two independent illumination optical paths. When the bright-field illumination source is enabled, the system acquires bright-field images. When the phase-contrast illumination source is enabled, the system acquires phase-contrast images. Since no physical movement of mechanical parts is required, and the light source switching is controlled solely by circuit signals, the single mode switching time can be controlled within 80 milliseconds, which can meet the "fast response" requirement of automated detection and prevent the target from moving during the shooting process, so that the target or the shape of the target captured by the bright field image and the phase contrast image are inconsistent. At the same time, the bright field optical path and the phase contrast optical path are independent optical channels without sharing a lens group. Traditional phase contrast microscopes have an annular aperture at the illumination end, which is usually located near the back focal plane of the condenser. After the annular aperture is imaged by the condenser and the objective lens, its size must match the symmetrical ring near the back focal plane of the phase contrast objective lens. Therefore, the illumination part of a traditional phase contrast microscope consists of an annular aperture and a condenser lens, and a centering adjustment structure for the annular aperture is required to make the annular aperture at the illumination end concentric with the symmetrical ring near the back focal plane of the objective lens. The phase contrast imaging illumination section of this invention uses only a high-brightness LED array as the light source, eliminating the need for additional components such as condenser lenses and annular aperture centering components. This not only reduces the number of core parts but also lowers the difficulty and cost of optical path assembly and adjustment. The bright-field optical path adopts a Köhler illumination design, and through the synergistic effect of the condenser lens, field aperture, and aperture stop, the illumination light forms uniform illumination on the sample plane and improves the contrast of cell images. The phase contrast objective lens can handle both bright-field and phase contrast imaging. The control software supports selecting bright-field imaging alone or switching between bright-field and phase contrast imaging based on function settings. Since simultaneously capturing bright-field and phase contrast images of the same target location would significantly reduce the testing speed, automatic identification of whether to capture phase contrast images is essential. The software provides manual and automatic shooting modes for this purpose. In manual mode, users can directly select to capture bright-field images or switch between capturing bright-field and phase contrast images. In automatic mode, the software automatically determines the shooting strategy after processing the image through an algorithm. It also supports linkage between two test modules, deciding whether to activate phase contrast image capture based on the results of other test units. Attached Figure Description

[0017] Figure 1 is a schematic diagram of the microscopic system of the present invention. Figure 2 is a schematic diagram of the LED chip array of the present invention packaged into an LED light source. Figure 3 is a schematic diagram of the LED light source composed of the packaged circular LED array of the present invention. Figure 4 shows the array LED chips of the present invention illuminated in a circular state after packaging. Figure 5 shows the array LEDs of the present invention illuminated in a circular state after packaging. Figure 6 is a schematic diagram of the present invention capturing a series of images from unclear to clear and then back to unclear before and after the focal point. Figure 7 is a schematic diagram of the three-dimensional structure of the present invention.

[0018] In the diagram: 1. Imaging module; 2. Bright-field imaging illumination module; 3. Focusing module; 4. Phase-contrast imaging illumination source module; 5. Control module; 6. Beam splitter; 7. Optical path; 8. Stage; 101. Phase-contrast objective lens; 102. Phase plate; 103. Lens barrel; 104. Image receiving device; 201. LED light source; 202. Condenser lens; 203. Field stop; 204. Aperture stop; 205. Condenser lens; 301. Stepper motor; 302. Connecting plate; 303. Optical coupler baffle; 304. Optical coupler. Detailed Implementation

[0019] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0020] To facilitate understanding of the present invention, a more comprehensive description of the invention will be given below with reference to the accompanying drawings, and several embodiments of the invention will be provided. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of the present invention will be more thorough and complete.

[0021] As illustrated in Figures 1-7, this invention provides a technical solution: a microscopic imaging system with bright-field imaging and phase-contrast imaging, comprising an imaging module 1, a bright-field imaging illumination module 2, a focusing module 3, a phase-contrast imaging illumination source module 4, and a control module 5. The bright-field imaging illumination module 2 and the phase-contrast imaging illumination source module 4 are converged into an optical path 7 by a beam splitter 6 to provide illumination for the imaging module 1. A stage 8 is disposed between the bright-field imaging illumination module 2 and the imaging module 1. The imaging module 1 includes a phase-contrast objective lens 101, and a control module is disposed below the phase-contrast objective lens 101. The lens barrel 103 has an image receiving device 104 located below it. The bright-field imaging illumination module 2 includes an LED light source 201, a condenser lens 202, a field stop 203, an aperture stop 204, and a condenser lens 205 arranged sequentially along the optical path 7. These components together form a Köhler illumination system. The focusing module 3 includes a stepper motor 301. A connecting plate 302 and an optical coupler baffle 303 are mounted on the outer wall of the stepper motor 301. The connecting plate is also mounted on the connecting plate. When the moving parts move, an optical coupler 304 is located below the optical coupler baffle 303.

[0022] The phase contrast imaging illumination source module 4 uses an LED array light source. The LED array used in the phase contrast imaging illumination source module 4 is a ring LED structure composed of single LEDs, or an LED array of arbitrary shape composed of multiple LEDs and the LED array is lit in a ring state. Alternatively, the LED array used in the phase contrast imaging illumination source module 4 is a single LED ring light source formed by multiple LED light-emitting chips arranged and packaged in a ring, or a single LED light source formed by multiple LED light-emitting chips arranged and packaged in an arbitrary shape and the LED light source is lit in a ring state. The LED light sources of both the bright field imaging illumination module 2 and the phase contrast imaging illumination source module 4 are white LED light sources.

[0023] The transmittance of the beam splitter 6 is greater than its reflectance. The control module 5 controls the distance between the phase contrast objective lens 101 and the target. A series of sample images are taken at a fixed distance in front of and behind the focal point of the phase contrast objective lens 101 for focus finding and image synthesis. The number of images taken is greater than or equal to 1 and less than or equal to 20.

[0024] The control module 5 controls the distance between the phase contrast objective lens 101 and the target to automatically perform focusing and image capture, and controls the illumination field imaging illumination module 2, the phase contrast imaging illumination source module 4 and the imaging module 1 to continuously capture bright field images and phase contrast images, or automatically determine and execute whether to capture phase contrast images based on input conditions.

[0025] Input conditions include the ability to determine whether to capture a phase difference image based on a bright field image, the ability to determine whether to capture a phase difference image based on other unit test results, and the ability to select whether to capture a phase difference image within the software.

[0026] The phase contrast objective 101 can be an infinity conjugate objective or a finite conjugate objective. For an infinity conjugate objective, a tube lens needs to be installed in the microscope tube 103 to facilitate imaging. For a finite conjugate objective, no lens needs to be installed in the microscope tube 103. The magnification of the sample by the imaging module 1 is consistent with the magnification marked on the objective. The image receiving device 104 uses a color camera.

[0027] Stepper motor 301 is connected to the component to be moved via connecting plate 302. Optical coupler baffle 303 is also mounted on the connecting plate. When the moving component moves, optical coupler baffle 303 will block optical coupler 304. Starting from the position of optical coupler 304, the distance between phase contrast objective lens 101 and the target is controlled. Control module 5 controls the focusing and shooting process. Before each sample image is captured, the focus needs to be found. Starting from the position of optical coupler 304, stepper motor 301 moves a fixed distance to bring phase contrast objective lens 101 to a specific distance below the sample focus and start capturing images. Stepper motor 301 captures one image with each step it moves until it has traveled a specific distance past the focus and stops capturing images. The captured images will include blurry images that have not reached the focus position, gradually clearer images as it approaches the focus position, clear images that have reached the focus position, and gradually blurry images that are moving away from the specific focus position. All images are saved for focus position analysis. When capturing samples, based on the focus position determined by the focus analysis, stepper motor 301 moves the phase contrast objective lens 101 to a distance of 10 steps from the focus position, and image capture begins. Capture continues until the lens has moved 10 steps past the focus position, at which point capture stops, and the captured images are saved and analyzed. From the saved images, the clearest image is analyzed by an algorithm, and the targets in that image are classified and counted. More preferably, image fusion is performed on a series of saved images to widen the depth of field range of the objective lens, allowing for better reconstruction of cells whose volume exceeds the depth of field and cells located at different levels, thus reducing missed detections and misclassifications.

[0028] Control module 5 controls the capture of brightfield images and phase contrast images. Depending on the actual needs, control module 5 can choose to capture only brightfield images. If there is any doubt about the captured brightfield image and a phase contrast image is needed for review, control module 5 can choose to capture only phase contrast images. If both brightfield and phase contrast images are needed, control module 5 can choose to switch between capturing brightfield and phase contrast images, that is, capture one brightfield image and then capture one phase contrast image for the same target.

[0029] During testing, 20 images are captured on a single sample. Due to the small imaging range of the objective lens, multiple positions are needed to meet the image capture requirements for a single sample. That is, after the first position is captured, the counting plate (containing the sample and placed on the stage) is moved slightly, and then the second position is captured. In this way, a total of 20 positions are captured. When the control module 5 starts working, it determines whether to start phase contrast imaging based on the bright field image. When phase contrast imaging is enabled, the remaining positions are captured using a method of switching between bright field and phase contrast imaging until images of all 20 positions are captured. Secondly, when the control module 5 is linked with the dry chemistry module, the whole machine test is performed simultaneously with the dry chemistry test and the microscopic image capture. After the dry chemistry occult blood test is completed, the microscopic image is captured at the 10th position. The algorithm analyzes the image results of the previous 10 positions. If it finds that they do not correspond to the dry chemistry occult blood test results, the phase contrast imaging function is enabled, and the remaining 10 positions are captured using a method of switching between bright field and phase contrast imaging.

[0030] Bright-field imaging illumination module 2 and phase-contrast imaging illumination source module 4 provide illumination to the sample under test via a common optical path 7 through a beam splitter 6. In this invention, the light emitted from bright-field imaging illumination module 2 is reflected by beam splitter 6 to illuminate the objective lens, while the light emitted from phase-contrast imaging illumination source module 4 is transmitted through beam splitter 6 to illuminate the phase-contrast imaging. The distance between phase-contrast imaging illumination source module 4 and phase-contrast objective lens 101 is much greater than the focal length of the objective lens. Preferably, the distance between phase-contrast imaging illumination source module 4 and phase-contrast objective lens 101 should be more than 10 times the focal length of phase-contrast objective lens 101. In this case, phase-contrast imaging illumination source module 4 is equivalent to light entering the objective lens from infinity, sharing a common juxtaposition with the phase-contrast rings near the back focal plane of the objective lens, which conforms to the principle of phase-contrast imaging. Because the LED light source in phase-contrast imaging illumination source module 4 has a large emission angle, its energy utilization rate is low. To meet the brightness requirements of phase-contrast imaging, the transmittance of beam splitter 6 should be greater than its reflectance. More preferably, the ratio of transmittance to reflectance is 9:1.

[0031] In addition to the above settings, it is also possible to have the bright-field imaging illumination module 2 provide illumination to the phase-contrast objective lens 101 via transmission, and the phase-contrast imaging illumination source module 4 provide illumination to the phase-contrast objective lens 101 via reflection. To meet the requirements of this optical path setting method, the transmittance to reflectance ratio of the beam splitter should be 1:9, and the distance from the phase-contrast imaging illumination source module 4 to the phase-contrast objective lens 101 should also be more than 10 times the focal length of the phase-contrast objective lens 101. This setting method is also within the protection scope of this patent.

[0032] The beam splitter 6 can be a beam splitter or a beam splitter prism; the stepper motor 301 is the power source, and is connected to the component to be moved via the connecting plate 302. The optical coupler baffle 303 is also mounted on the connecting plate 302. When the moving component moves, the optical coupler baffle 303 will block the optical coupler 304. Taking the position of the optical coupler 304 as the starting point, the distance between the phase contrast objective lens 101 and the target is controlled. The focusing and shooting process is controlled by the control module 5. Before each sample image is captured, the focus needs to be found. Starting from the position of the optical coupler 304, the stepper motor 301 moves a fixed distance, so that the phase contrast objective lens 101 is at a specific distance below the sample focus, and the image is captured. Each step of the stepper motor captures one image until it has moved past the specific distance of the focus, and then the capturing stops. The captured images will include blurry images that have not reached the focus position, gradually clearer images as they approach the focus position, clear images that have reached the focus position, and gradually blurry images that are moving away from the specific focus position. All images are saved. The image is used to analyze the focal position. When capturing a sample, based on the focal position determined by the focusing analysis, the stepper motor 301 moves the phase contrast objective lens 101 to a distance of 10 steps from the focal position, and image capture begins. Capture continues until the lens has moved 10 steps past the focal position, at which point the capture stops, and the captured images are saved and analyzed. From the saved images, the algorithm analyzes and selects the clearest image, classifying and counting the targets in that image. More preferably, image fusion is performed on a series of saved images to widen the depth of field of the objective lens, better restoring cells whose volume exceeds the depth of field and cells located at different levels, thus reducing missed detections and misclassifications. The control module 5 controls the activation of phase contrast image capture. It determines whether to activate phase contrast image capture based on brightfield images. The control module 5 has three basic operating modes: 1. The control module 5 only captures brightfield images; 2. When there is doubt about the captured brightfield image and phase contrast image re-examination is required, the control module 5 only captures phase contrast images; 3.Control module 5 performs switching between bright-field and phase-contrast image capture, i.e., capturing one bright-field image followed by one phase-contrast image of the same target. This switching is achieved by comprehensively analyzing the bright-field image and dry chemical analysis results to determine whether to capture a phase-contrast image. The bright-field image analysis is combined with the results from the urine dry chemical analysis module to jointly decide whether to capture a phase-contrast image. In urine dry chemical analysis, urine samples are dropped onto a dozen or so dry chemical test strips, each representing a test item. The intensity of the color development on the urine sample and test strips indicates the concentration of the analyte chemical in the urine. The color development of some items is directly related to the microscopic image of the urine sample; for example, in the occult blood test, the result of the occult blood test is... The result was positive, but no red blood cell images were found in the tangible analysis. This situation may be due to the presence of shadow erythrocytes in the urine. Shadow erythrocytes refer to red blood cells that have dissolved in urine, with hemoglobin leaking out, leaving only an empty cell membrane shell. This is one of the typical forms of glomerular hematuria. Clinically, it is required that the results of the urine dry chemical occult blood test be cross-referenced with the results of urine microscopic imaging. Shadow erythrocytes have low contrast and are not easily detected in bright-field microscopy, but they are very obvious in phase-contrast imaging. Therefore, the occult blood test result is positive, but no red blood cells are found in bright-field imaging. In this case, control module 5 analyzes the above results, simultaneously acquiring a phase-contrast image while obtaining a bright-field image, and analyzes the combined results to determine whether shadow erythrocytes are present.

[0033] All standard parts used in this application can be purchased from the market. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art and are also general components, which are common knowledge in this field.

[0034] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microscopic imaging system with bright-field imaging and phase-contrast imaging, comprising an imaging module (1), a bright-field imaging illumination module (2), a focusing module (3), a phase-contrast imaging illumination source module (4), and a control module (5), characterized in that: The phase contrast imaging illumination source module (4) and the bright field imaging illumination module (2) are provided with a stage (8); the imaging module (1) includes a phase contrast objective lens (101) and an image receiving device (104); the control module (5) controls the distance between the phase contrast objective lens (101) and the target to automatically perform focusing and image shooting, and controls the bright field imaging illumination module (2), the phase contrast imaging illumination source module (4), and the imaging module (1) to continuously shoot bright field images and phase contrast images or automatically determine and execute whether to shoot phase contrast images according to input conditions.

2. The microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The phase difference imaging illumination source module (4) adopts an LED ring array light source, and the LED light source is white.

3. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The LED ring array light source is a ring structure composed of single LEDs, or an array composed of multiple LEDs, wherein the multiple LEDs are configured to be lit in a ring.

4. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The LED light source of the bright field imaging illumination module (2) is a white LED light source.

5. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The bright field imaging illumination module (2) includes an LED light source (201), a light-collecting lens (202), a field aperture (203), an aperture stop (204), and a condenser lens (205) arranged sequentially along the optical path (7), and the above components together form a Köhler illumination system.

6. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The focusing module (3) is connected to the imaging module (1), and the control module (5) controls the movement of the imaging module (1) to make the phase contrast objective (101) move closer to and further away from the target.

7. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The control module (5) controls the distance between the phase contrast objective (101) and the target, and takes a series of target images at a fixed distance in front of and behind the focal point of the phase contrast objective (101) for focus finding and image synthesis. The number of images taken is greater than or equal to 1 and less than or equal to 20.

8. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The phase contrast objective (101) can be an infinity conjugate objective or a finite conjugate objective. For the infinity conjugate objective, a tube lens is set between the image receiving device (104) and the phase contrast objective (101) to cooperate with imaging. For finite distance conjugate objectives, there is no need to set up a lens in the lens barrel (103).

9. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The image receiving device (104) employs a color camera.

10. A microscopic imaging system with bright-field imaging and phase-contrast imaging according to claim 1, characterized in that: The input conditions include the ability to determine whether to capture a phase difference image based on a bright field image, the ability to determine whether to capture a phase difference image based on unit test results, and the ability to select whether to capture a phase difference image in the software.

Images