Aseptic high pressure high speed rotation cuts goat milk into small molecule process

Abstract

This invention discloses a sterile, high-pressure, high-speed rotary process for breaking down goat milk into smaller molecules, belonging to the field of food processing technology. The process includes the following steps: after pretreatment, pasteurization, and homogenization, the goat milk undergoes ultra-high pressure homogenization. A homogenizing valve cutting component passes through the milk at high pressure and high flow rate, breaking down large molecules into smaller molecules. Hydrophobically modified xanthan gum-gellan gum-chitosan composite lactase microcapsules are prepared and added to the small-molecule goat milk at a mass ratio of 4‰-6‰ for enzymatic hydrolysis. The mixture is then filtered to obtain sterile, low-lactose, small-molecule goat milk. Ultra-high pressure homogenization achieves the downsizing of goat milk, improving its digestibility and absorption. The composite microcapsule encapsulation of lactase enables efficient lactose hydrolysis. Hydrophobic modification of xanthan gum enhances the encapsulation efficiency and operational stability of the microcapsules. This invention provides a continuous and sterile process, ensuring adequate aseptic protection. The resulting product combines easy absorption due to its small molecular size with low lactose content, making it suitable for lactose-intolerant individuals and those with weak digestive systems.

Description

Technical Field

[0001] This invention relates to the field of food processing technology, specifically a process for cutting goat milk into small molecules using aseptic high-pressure high-speed rotation. Background Technology

[0002] Goat milk is rich in protein, fat, vitamins, and minerals, and its allergenicity is lower than that of cow milk. However, there are two major technical bottlenecks in the practical application of goat milk: First, the fat globules in goat milk are relatively large and contain a high proportion of αs1-casein, which makes it difficult for some people, especially infants and the elderly, to digest and absorb, and is accompanied by a noticeable goaty smell; Second, goat milk has a high lactose content, and the incidence of lactose intolerance in Asian populations is as high as 90%, which can easily cause discomfort such as bloating and diarrhea after consumption, seriously limiting the consumer base for goat milk products.

[0003] To address the issue of large molecular particles in goat milk, existing technologies typically employ conventional homogenization (pressure 20-50 MPa) for physical disruption. However, under these pressures, only fat globules can be reduced to 1-2 μm in size, failing to effectively break down protein aggregates. Furthermore, the inactivation effect on endogenous enzymes naturally present in goat milk, such as lipases and proteases, is limited, leading to fat bubbling and protein coagulation during storage. Regarding lactose intolerance, current technologies often involve the direct addition of free lactase for hydrolysis or the removal of lactose through membrane separation technologies such as ultrafiltration and nanofiltration. While adding free enzymes is simple, the enzymes cannot be recycled, resulting in higher production costs, and enzyme protein residues may pose an allergy risk. Membrane separation technology, while effective in removing lactose, also loses some minerals and active ingredients, impacting the natural nutritional value of goat milk.

[0004] To address the aforementioned problems, this invention provides a sterile, high-pressure, high-speed rotating process for cutting sheep milk into smaller molecules. Summary of the Invention

[0005] The purpose of this invention is to provide a sterile, high-pressure, high-speed rotating process for cutting sheep milk into small molecules, thereby solving the problems raised in the prior art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: The aseptic, high-pressure, high-speed rotation process for breaking down goat milk into smaller molecules includes the following steps: Step 1: Small molecule cutting process for raw goat milk Raw sheep milk is filtered to obtain filtered sheep milk. The filtered sheep milk is preheated to 50-60℃, and impurities are separated to obtain pretreated sheep milk. The pretreated sheep milk is pasteurized and homogenized, and then cooled after sterilization to obtain processed aseptic sheep milk. The processed aseptic sheep milk is preheated and homogenized under high pressure feeding at high temperature and ultra-high pressure. After cooling, it is stored in a sterile environment to obtain cut small molecule sheep milk. Step 2: Preparation of aseptic, low-lactose, small-molecule goat milk Adding compound lactase microcapsules to small molecule goat milk and hydrolyzing it at a constant temperature, followed by filtration, yields sterile, low-lactose small molecule goat milk.

[0007] Furthermore, in step one, the specific process for processing raw sheep milk is as follows: the freshly collected raw sheep milk is filtered through a 60-100 mesh at 2-6℃ to remove impurities, the filtered sheep milk is preheated to 50-60℃, and then separated and impurities are removed by a separator at a speed of 3000-8000r / min to obtain pretreated sheep milk.

[0008] Furthermore, in step one, the specific process of pasteurization and homogenization is as follows: sterilize at 80-86℃ for 10-15 seconds, homogenize at a pressure of 18-20 MPa, cool to 4-8℃ after sterilization, and temporarily store in an aseptic tank; thus obtaining the processed aseptic goat milk.

[0009] Furthermore, in step one, the specific process of ultra-high pressure homogenization is as follows: the homogenization pressure is set to 200-300MPa, and the sheep milk is passed through a homogenization valve with a gap of 8-10μm at a flow rate of 200-205m / s. The cutting temperature is raised to 65-70℃. The milk is then cooled to 20-30℃ and stored in an aseptic tank to obtain the cut small molecule sheep milk.

[0010] Furthermore, in step two, the preparation process of the composite lactase microcapsules is as follows: hydrophobically modified xanthan gum powder and gellan gum powder are dissolved in deionized water and stirred thoroughly to obtain a mixed gum solution; β-galactosidase is added to the above mixed gum solution to obtain a mixed gum-enzyme mixture; the mixed gum-enzyme mixture is added dropwise to the chitosan solution at a uniform rate; after the addition is complete, stirring is continued slowly; and the mixture is sieved to obtain composite lactase microcapsules.

[0011] Furthermore, the preparation method of the hydrophobically modified xanthan gum is as follows: xanthan gum is taken, dissolved in deionized water, the pH value is adjusted to 11-11.2, stirred evenly, vacuum dried, pulverized, and sieved to obtain xanthan gum powder; hexadecyl glycidyl ether is dissolved in a mixed solvent to obtain a mixed solution; xanthan gum powder is taken, and the mixed solution is sprayed into it, stirred continuously, and heated to react; after the reaction is completed, it is washed with anhydrous ethanol and vacuum dried to obtain hydrophobically modified xanthan gum.

[0012] Furthermore, the mixed adhesive solution comprises, by mass fraction: 4wt%-5wt% xanthan gum powder, 4wt%-5wt% gellan gum powder, and the balance being deionized water.

[0013] Furthermore, the chitosan solution is prepared by dissolving chitosan in a 1% aqueous solution of glacial acetic acid. After complete dissolution, 0.1 mol / L sodium hydroxide solution is added dropwise to slowly adjust the pH to 6.0-6.2, thereby obtaining a chitosan solution with a mass concentration of 1%.

[0014] Furthermore, in step two, the specific parameters for isothermal enzymatic hydrolysis are: isothermal enzymatic hydrolysis at 45-55℃ for 3-5 hours.

[0015] Furthermore, in step two, the amount of compound lactase microcapsules added is 4‰-6‰ of the mass of small molecule sheep milk.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention employs ultra-high pressure homogenization technology of 200-300 MPa, allowing goat milk to flow at a rate exceeding 200 m / s through a homogenizing valve cutting assembly with a gap of only 8-10 μm. Under these extreme conditions, the large molecular particles in the goat milk are subjected to intense mechanical shearing forces, achieving highly efficient physical fragmentation. The average particle size of the processed goat milk is significantly reduced, and the particle size distribution is more uniform, forming a stable small molecular cluster emulsion. This physical molecule-reducing treatment not only improves the taste and stability of the goat milk, but more importantly, it significantly enhances its digestibility and absorption rate, making it particularly suitable for infants, the elderly, and people with weak digestive systems.

[0017] 2. This invention employs comprehensive sterilization during the production process, including preheating and filtration, pasteurization, standard homogenization, and high-pressure, high-speed homogenization. Throughout the process, high pressure is maintained to prevent external air infiltration. This multi-dimensional aseptic system ensures that the goat milk remains sterile throughout the ultra-high-pressure cutting process.

[0018] 3. This invention utilizes β-galactosidase microcapsules to enzymatically hydrolyze small-molecule goat milk after it has been cut. The lactase in the microcapsules efficiently hydrolyzes lactose in goat milk into easily absorbed glucose and galactose under mild conditions, significantly reducing lactose content and effectively alleviating lactose intolerance symptoms. Simultaneously, the monosaccharides generated by hydrolysis impart a natural sweetness to the goat milk, improving its taste and masking its characteristic goaty odor without the need for additional sweeteners. The enzymatic hydrolysis process is carried out at a mild temperature, avoiding the destruction of heat-sensitive active substances in goat milk by high temperatures, thus preserving the natural nutritional value of goat milk to the greatest extent while reducing lactose.

[0019] 4. This invention achieves a synergistic effect by hydrophobically modifying xanthan gum with hexadecyl glycidyl ether and using it as a microcapsule wall material. The hydrophobically modified xanthan gum, while maintaining its original gel properties, introduces long-chain alkyl groups, forming hydrophobic microdomains within the microcapsules. These microdomains, through hydrophobic interactions, exhibit affinity adsorption with lactase molecules, significantly improving the encapsulation efficiency and operational stability of the microcapsules for lactase. The hydrophobic long chains on the surface of the modified xanthan gum have good affinity for fat globules in sheep milk, resulting in more uniform dispersion of the microcapsules in the sheep milk system, reducing sedimentation or floating. Simultaneously, it plays a role in auxiliary emulsification and stabilization, further enhancing product stability and demonstrating a good ability to reduce lactose digestibility. Detailed Implementation

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments, and all described quantities are by weight. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] The sources and types of substances involved in this invention are not particularly limited. Exemplary examples include the following raw materials: xanthan gum: item number: S30551; gellan gum: item number: S30511, which can be purchased from Shanghai Yuanye Biotechnology Co., Ltd. Example

[0022] Step 1: Small molecule cutting process for raw goat milk Freshly collected raw sheep milk was filtered through a 60-mesh filter at 2°C to remove impurities. The filtered sheep milk was then preheated to 50°C and separated by a separator at a speed of 3000 r / min to remove impurities, thus obtaining pretreated sheep milk. The pretreated goat milk was pasteurized and homogenized: it was sterilized at 80℃ for 10 seconds, homogenized at a pressure of 18 MPa, and then cooled to 4℃ and temporarily stored in an aseptic tank to obtain the processed aseptic goat milk. The processed aseptic goat milk is preheated to 40°C and fed into an ultra-high pressure homogenizer via a high-pressure feed pump. The homogenization pressure is set to 200MPa, and the goat milk is fed through a homogenization valve cutting assembly with a gap of 8μm at a flow rate of 200m / s, raising the temperature to 65°C. The milk is then cooled to 20°C and stored in an aseptic tank to obtain the cut small molecule goat milk. Step 2: Preparation of compound lactase microcapsules Weigh 10g xanthan gum and dissolve it in 500mL of deionized water. Then, add 0.5mol / L sodium hydroxide solution to adjust the pH to 11.1. Stir well, dry in a vacuum oven, pulverize, and sieve to obtain xanthan gum powder. Dissolve 3.7g cetyl glycidyl ether in a mixed solvent of 75mL tetrahydrofuran and 25mL deionized water to obtain a mixed solution. Weigh the xanthan gum powder and spray the mixed solution into a flask using a spray device. Stir continuously and heat to 45℃ under reflux for 3 hours. Wash with anhydrous ethanol and dry under vacuum to obtain hydrophobically modified xanthan gum. Dissolve 1g of chitosan in a 1wt% aqueous solution of glacial acetic acid. After complete dissolution, slowly adjust the pH to 6.0 by adding 0.1mol / L sodium hydroxide solution dropwise to obtain a 1wt% chitosan solution. Take 0.5g of hydrophobically modified xanthan gum powder and 0.5g of gellan gum powder, dissolve them in 100mL of deionized water, and stir thoroughly to obtain a mixed gum solution; take 50mL of the above mixed gum solution and add 1.0g of β-galactosidase to obtain a mixed gum-enzyme mixture; using a constant flow pump, add the mixed gum-enzyme mixture dropwise at a constant speed of 300r / min to a slowly stirred 1wt% chitosan solution; after the addition is complete, continue to stir slowly for 30min; sieve to obtain composite lactase microcapsules; Step 3: Preparation of aseptic, low-lactose, small-molecule goat milk Small molecule goat milk was placed in sterile containers, and 4‰ of compound lactase microcapsules were added. The mixture was enzymatically hydrolyzed at 45℃ for 3 hours and then filtered to obtain sterile low-lactose small molecule goat milk. Example

[0023] Step 1: Small molecule cutting process for raw goat milk Freshly collected raw sheep milk is filtered through an 80-mesh filter at 4°C to remove impurities. The filtered sheep milk is then preheated to 55°C and separated by a separator at a speed of 5000 r / min to remove impurities, thus obtaining pretreated sheep milk. The pretreated goat milk was pasteurized and homogenized: it was sterilized at 83°C for 12 seconds and homogenized at a pressure of 19 MPa. After sterilization, it was cooled to 6°C and temporarily stored in an aseptic tank to obtain the processed aseptic goat milk. The processed aseptic goat milk is preheated to 50°C and fed into an ultra-high pressure homogenizer via a high-pressure feed pump. The homogenization pressure is set to 250 MPa, and the goat milk is fed through a homogenization valve cutting assembly with a gap of 9 μm at a flow rate of 200 m / s, raising the temperature to 68°C. The milk is then cooled to 25°C and stored in an aseptic tank to obtain the cut small molecule goat milk. Step 2: Preparation of compound lactase microcapsules Weigh 10g xanthan gum and dissolve it in 500mL of deionized water. Then, add 0.5mol / L sodium hydroxide solution to adjust the pH to 11. Stir well, dry in a vacuum oven, pulverize, and sieve to obtain xanthan gum powder. Dissolve 3.7g cetyl glycidyl ether in a mixed solvent of 75mL tetrahydrofuran and 25mL deionized water to obtain a mixed solution. Weigh the xanthan gum powder and spray the mixed solution into a flask using a spray device. Stir continuously and heat to 50℃ under reflux for 3.5h. Wash with anhydrous ethanol and dry under vacuum to obtain hydrophobically modified xanthan gum. Dissolve 1g of chitosan in a 1wt% aqueous solution of glacial acetic acid. After complete dissolution, slowly adjust the pH to 6.0 by adding 0.1mol / L sodium hydroxide solution dropwise to obtain a 1wt% chitosan solution with pH 6. Take 0.45g of hydrophobically modified xanthan gum powder and 0.45g of gellan gum powder, dissolve them in 100mL of deionized water, and stir thoroughly to obtain a mixed gum solution; take 50mL of the above mixed gum solution, add 1.0g of β-galactosidase to obtain a mixed gum-enzyme mixture; using a constant flow pump, add the mixed gum-enzyme mixture dropwise at a constant speed of 500r / min to a slowly stirred 1wt% chitosan solution; after the addition is complete, continue to stir slowly for 50min; sieve to obtain composite lactase microcapsules; Step 3: Preparation of aseptic, low-lactose, small-molecule goat milk Small molecule goat milk was placed in sterile containers, and 5‰ of compound lactase microcapsules were added. The mixture was enzymatically hydrolyzed at 50℃ for 4 hours and then filtered to obtain sterile low-lactose small molecule goat milk. Example

[0024] Step 1: Small molecule cutting process for raw goat milk Freshly collected raw sheep milk is filtered through a 100-mesh filter at 6°C to remove impurities. The filtered sheep milk is then preheated to 60°C and separated by a separator at 8000 r / min to remove impurities, thus obtaining pretreated sheep milk. The pretreated goat milk was pasteurized and homogenized: it was sterilized at 86℃ for 15 seconds, homogenized at a pressure of 20 MPa, and then cooled to 8℃ and temporarily stored in an aseptic tank to obtain the processed aseptic goat milk. The processed aseptic goat milk is preheated to 60°C and fed into an ultra-high pressure homogenizer via a high-pressure feed pump. The homogenization pressure is set to 300MPa, and the goat milk is fed through a homogenization valve cutting assembly with a gap of 10μm at a flow rate of 200m / s, raising the temperature to 70°C. The milk is then cooled to 30°C and stored in an aseptic tank to obtain the cut small molecule goat milk. Step 2: Preparation of compound lactase microcapsules Weigh 10g xanthan gum and dissolve it in 500mL of deionized water. Then, add 0.5mol / L sodium hydroxide solution to adjust the pH to 11. Stir well, dry in a vacuum oven, pulverize, and sieve to obtain xanthan gum powder. Dissolve 3.7g cetyl glycidyl ether in a mixed solvent of 75mL tetrahydrofuran and 25mL deionized water to obtain a mixed solution. Weigh the xanthan gum powder and spray the mixed solution into a flask using a spray device. Stir continuously and heat to 55℃ under reflux for 4 hours. Wash with anhydrous ethanol and dry under vacuum to obtain hydrophobically modified xanthan gum. Dissolve 1g of chitosan in a 1wt% aqueous solution of glacial acetic acid. After complete dissolution, slowly adjust the pH to 6.2 by adding 0.1mol / L sodium hydroxide solution dropwise to obtain a 1wt% chitosan solution. Take 0.4 g of hydrophobically modified xanthan gum powder and 0.4 g of gellan gum powder, dissolve them in 100 mL of deionized water, and stir thoroughly to obtain a mixed gum solution; take 50 mL of the above mixed gum solution, add 1.0 g of β-galactosidase to obtain a mixed gum-enzyme mixture; use a constant flow pump to add the mixed gum-enzyme mixture dropwise at a constant speed of 600 r / min to a slowly stirred 1 wt% chitosan solution; after the addition is complete, continue to stir slowly for 60 min; sieve to obtain composite lactase microcapsules; Step 3: Preparation of aseptic, low-lactose, small-molecule goat milk Small molecule goat milk was placed in sterile containers, and 6‰ of compound lactase microcapsules were added. The mixture was enzymatically hydrolyzed at 55℃ for 5 hours and then filtered to obtain sterile low-lactose small molecule goat milk.

[0025] Comparative Example 1: Conventional homogenization was used instead of ultra-high pressure homogenization; otherwise, it was the same as in Example 1. Step 1: Small molecule cutting process for raw goat milk Freshly collected raw sheep milk was filtered through a 60-mesh filter at 2°C to remove impurities. The filtered sheep milk was then preheated to 50°C and separated by a separator at a speed of 3000 r / min to remove impurities, thus obtaining pretreated sheep milk. The pretreated goat milk was pasteurized and homogenized: it was sterilized at 80℃ for 10 seconds, homogenized at a pressure of 18 MPa, and then cooled to 4℃ and temporarily stored in an aseptic tank to obtain the processed aseptic goat milk. The processed aseptic goat milk was preheated to 40°C and then homogenized in two stages using a high-pressure homogenizer. The first stage homogenization pressure was 20 MPa and the second stage homogenization pressure was 5 MPa. The homogenized goat milk was cooled to 4-8°C and temporarily stored in an aseptic tank for later use, thus obtaining homogenized goat milk. Step 2: Preparation of compound lactase microcapsules Weigh 10g xanthan gum and dissolve it in 500mL of deionized water. Then, add 0.5mol / L sodium hydroxide solution to adjust the pH to 11.1. Stir well, dry in a vacuum oven, pulverize, and sieve to obtain xanthan gum powder. Dissolve 3.7g cetyl glycidyl ether in a mixed solvent of 75mL tetrahydrofuran and 25mL deionized water to obtain a mixed solution. Weigh the xanthan gum powder and spray the mixed solution into a flask using a spray device. Stir continuously and heat to 45℃ under reflux for 3 hours. Wash with anhydrous ethanol and dry under vacuum to obtain hydrophobically modified xanthan gum. Dissolve 1g of chitosan in a 1wt% aqueous solution of glacial acetic acid. After complete dissolution, slowly adjust the pH to 6.0 by adding 0.1mol / L sodium hydroxide solution dropwise to obtain a 1wt% chitosan solution. Take 0.5g of hydrophobically modified xanthan gum powder and 0.5g of gellan gum powder, dissolve them in 100mL of deionized water, and stir thoroughly to obtain a mixed gum solution; take 50mL of the above mixed gum solution and add 1.0g of β-galactosidase to obtain a mixed gum-enzyme mixture; using a constant flow pump, add the mixed gum-enzyme mixture dropwise at a constant speed of 300r / min to a slowly stirred 1wt% chitosan solution; after the addition is complete, continue to stir slowly for 30min; sieve to obtain composite lactase microcapsules; Step 3: Preparation of aseptic, low-lactose, small-molecule goat milk The homogenized goat milk was placed in sterile containers, and 4‰ of compound lactase microcapsules were added. The mixture was enzymatically hydrolyzed at 45℃ for 3 hours and then filtered to obtain sterile low-lactose small molecule goat milk.

[0026] Comparative Example 2: Unmodified xanthan gum was used, without hydrophobic modified xanthan gum; all other aspects were the same as in Example 1. Specifically: Step 1: Small molecule cutting process for raw goat milk Freshly collected raw sheep milk was filtered through a 60-mesh filter at 2°C to remove impurities. The filtered sheep milk was then preheated to 50°C and separated by a separator at a speed of 3000 r / min to remove impurities, thus obtaining pretreated sheep milk. The pretreated goat milk was pasteurized and homogenized: it was sterilized at 80℃ for 10 seconds, homogenized at a pressure of 18 MPa, and then cooled to 4℃ and temporarily stored in an aseptic tank to obtain the processed aseptic goat milk. The processed aseptic goat milk is preheated to 40°C and fed into an ultra-high pressure homogenizer via a high-pressure feed pump. The homogenization pressure is set to 200MPa, and the goat milk is fed through a homogenization valve cutting assembly with a gap of 8μm at a flow rate of 200m / s, raising the temperature to 65°C. The milk is then cooled to 20°C and stored in an aseptic tank to obtain the cut small molecule goat milk. Step 2: Preparation of compound lactase microcapsules Dissolve 1g of chitosan in a 1wt% aqueous solution of glacial acetic acid. After complete dissolution, slowly adjust the pH to 6.0 by adding 0.1mol / L sodium hydroxide solution dropwise to obtain a 1wt% chitosan solution. Take 0.5g xanthan gum powder and 0.5g gellan gum powder, dissolve them in 100mL deionized water, and stir thoroughly to obtain a mixed gum solution; take 50mL of the above mixed gum solution, add 1.0g β-galactosidase to obtain a mixed gum-enzyme mixture; use a constant flow pump to add the mixed gum-enzyme mixture dropwise at a constant speed of 300r / min to a slowly stirred 1wt% chitosan solution; after the addition is complete, continue to stir slowly for 30min; sieve to obtain composite lactase microcapsules; Step 3: Preparation of aseptic, low-lactose, small-molecule goat milk Small molecule goat milk was placed in sterile containers, and 4‰ of compound lactase microcapsules were added. The mixture was enzymatically hydrolyzed at 45℃ for 3 hours and then filtered to obtain sterile low-lactose small molecule goat milk.

[0027] Comparative Example 3: Enzymatic hydrolysis was performed first, followed by cutting; the rest was the same as in Example 1.

[0028] Comparative Example 4: Instead of using compound lactase microcapsules, β-galactosidase was added directly, and the rest was the same as in Example 1.

[0029] experiment: (1) Microbial determination (commercial sterility): Sterility testing was conducted according to GB-4789.26-2003. One sample from each batch was stored in a refrigerator at 2℃~5℃ as a control. The remaining samples were incubated at 36℃±1℃ for 10 days and then subjected to sensory evaluation, pH testing, and microscopic examination of smear staining. If no signs of microbial proliferation were found, the samples were determined to be commercially sterile. The data obtained are shown in Table 1 below: (2) The lactose content of the lactose-free milk obtained in Examples 1-3 and Comparative Examples 1-4 was detected, and the data are shown in Table 1 below:

[0030] Conclusion: The data above show that the sterile, low-lactose, small-molecule goat milk prepared by this invention meets commercial sterility standards, with a lactose content of less than 0.05 g / 100 g. This indicates that the process of this invention has good sterility assurance and efficient lactose hydrolysis. After ultra-high pressure homogenization, the particle size of the goat milk is significantly reduced, forming a stable small-molecule cluster emulsion, indicating that the process of this invention can effectively improve the digestibility and absorption performance of goat milk.

[0031] Comparative Example 1 uses conventional homogenization instead of ultra-high pressure homogenization. This process does not include the ultra-high pressure cutting step of 200-300MPa. Compared with the example, the product did not meet the commercial sterility standard, indicating that the instantaneous high temperature (120-160℃) generated by ultra-high pressure homogenization plays a key role in killing microorganisms and ensuring product sterility.

[0032] Comparative Example 2 used unmodified xanthan gum instead of hydrophobically modified xanthan gum to prepare microcapsules. The wall material of this microcapsule did not contain hexadecyl hydrophobic chains. Compared with the example, the lactose content of the product was significantly increased, indicating that the hydrophobically modified xanthan gum enhanced the encapsulation and protection of lactase by forming hydrophobic microregions, thus avoiding the decrease in hydrolysis efficiency caused by enzyme leakage.

[0033] Comparative Example 3 uses a process sequence of enzymatic hydrolysis followed by cutting. In this process, the microcapsules are destroyed during ultra-high pressure homogenization. Compared with the example, the lactose content of the product is close to that of the raw sheep milk, indicating that the process sequence of the present invention is more reasonable and ensures that lactase can fully exert its hydrolytic effect under mild conditions.

[0034] Comparative Example 4 did not employ a microencapsulation strategy; instead, free lactase was directly added to goat milk for enzymatic hydrolysis. This process did not include a microencapsulation step for lactase, and the lactose content was slightly higher than in the Example 4. The reason for this is that the free enzyme, lacking the protection of microcapsules during hydrolysis, is easily deactivated by the pH and temperature of the goat milk system, as well as by its own proteolytic enzymes, leading to a slight decrease in lactose hydrolysis efficiency.

[0035] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

Claims

1. A sterile, high-pressure, high-speed rotating process for cutting goat milk into small molecules, characterized by: Includes the following steps: Step 1: Small molecule cutting process for raw goat milk The raw sheep milk is filtered to obtain filtered sheep milk. The filtered sheep milk is preheated to 50-60℃ and separated to remove impurities, thus obtaining pretreated sheep milk. The pretreated goat milk is pasteurized and homogenized, then cooled after sterilization to obtain aseptic goat milk. The aseptic goat milk is preheated and homogenized under high pressure by high-temperature and ultra-high pressure feeding. After cooling, it is stored in an aseptic environment to obtain cut small molecule goat milk. Step 2: Preparation of aseptic, low-lactose, small-molecule goat milk Adding compound lactase microcapsules to small molecule goat milk and hydrolyzing it at a constant temperature, followed by filtration, yields sterile, low-lactose small molecule goat milk.

2. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 1, characterized in that: In step one, the specific process of processing raw sheep milk is as follows: Freshly collected raw sheep milk is filtered through a 60-100 mesh at 2-6℃ to remove impurities. The filtered sheep milk is preheated to 50-60℃ and then separated and impurities are removed by a separator at a speed of 3000-8000r / min to obtain pre-treated sheep milk.

3. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 1, characterized in that: In step one, the specific process of pasteurization and homogenization is as follows: sterilize at 80-86℃ for 10-15 seconds, homogenize at a pressure of 18-20 MPa, cool to 4-8℃ after sterilization, and temporarily store in an aseptic tank; thus obtaining the processed aseptic goat milk.

4. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 1, characterized in that: In step one, the specific process of ultra-high pressure homogenization is as follows: the homogenization pressure is set to 200-300MPa, and the sheep milk is passed through a homogenization valve with a gap of 8-10μm at a flow rate of 200-205m / s to cut it, and the temperature is raised to 65-70℃; it is then cooled to 20-30℃ and stored in an aseptic tank to obtain the cut small molecule sheep milk.

5. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 1, characterized in that: In step two, the preparation process of the composite lactase microcapsules is as follows: hydrophobically modified xanthan gum powder and gellan gum powder are dissolved in deionized water and stirred thoroughly to obtain a mixed gum solution; β-galactosidase is added to the above mixed gum solution to obtain a mixed gum-enzyme mixture; the mixed gum-enzyme mixture is added dropwise to the chitosan solution at a uniform rate; after the addition is complete, stirring is continued slowly; the mixture is sieved to obtain composite lactase microcapsules.

6. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 5, characterized in that: The preparation method of the hydrophobically modified xanthan gum is as follows: xanthan gum is dissolved in deionized water, the pH value is adjusted to 11-11.2, stirred evenly, vacuum dried, pulverized, and sieved to obtain xanthan gum powder; hexadecyl glycidyl ether is dissolved in a mixed solvent to obtain a mixed solution; xanthan gum powder is then sprayed into the mixed solution. The mixture was stirred continuously and heated to react. After the reaction was completed, the xanthan gum was washed with anhydrous ethanol and dried under vacuum to obtain hydrophobically modified xanthan gum.

7. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 5, characterized in that: The mixed adhesive solution consists of the following components by mass fraction: 4wt%-5wt% xanthan gum powder, 4wt%-5wt% gellan gum powder, and the balance being deionized water.

8. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 5, characterized in that: The chitosan solution is prepared by dissolving chitosan in a 1% aqueous solution of glacial acetic acid. After complete dissolution, 0.1 mol / L sodium hydroxide solution is added dropwise to slowly adjust the pH to 6.0-6.2, resulting in a chitosan solution with a mass concentration of 1%.

9. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 1, characterized in that: In step two, the specific parameters for isothermal enzymatic hydrolysis are: isothermal enzymatic hydrolysis at 45-55℃ for 3-5 hours.

10. The aseptic high-pressure high-speed rotation process for cutting goat milk into small molecules according to claim 1, characterized in that: In step two, the amount of compound lactase microcapsules added is 4‰-6‰ of the mass of small molecule sheep milk.

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