Application of bio-organic fertilizer produced from by-products in sugar industry in improvement of tomato yield and / or quality

Abstract

The invention relates to a divisional application of a Chinese invention patent application with an application number of 202311209611. 2, an application date of September 19, 2023, and an invention name of a bio-organic fertilizer produced by using sugar industry byproducts and a preparation method thereof. The invention discloses an application of a bio-organic fertilizer produced by using by-products in the sugar industry in improvement of tomato yield and / or quality, and belongs to the technical field of bio-organic fertilizers. The invention provides an application of a bio-organic fertilizer produced by using by-products in the sugar industry in improvement of tomato yield and / or quality. The bio-organic fertilizer produced by utilizing the by-product of the sugar industry is prepared from the following components in parts by mass: 30 to 40 parts of water-soluble calcium humate, 20 to 35 parts of filter mud fermentation product, 15 to 25 parts of magnesium nitrate hexahydrate, 0.4 to 1 part of boric acid, 0.4 to 1 part of zinc sulfate, 1 to 2 parts of sodium molybdate, 1 to 2 parts of amino acid, 1 to 2 parts of dioctyl sodium sulfosuccinate, 0.5 to 1 part of D-glucuronic acid and 1 to 2 parts of sodium polyacrylate. The yield, quality and appearance of the tomatoes can be improved to different degrees by respectively spraying the bio-organic fertilizer in the early flowering stage, the fruit swelling stage and the color changing stage of the tomatoes.

Description

[0001] This invention is a divisional application of Chinese invention patent application No. 202311209611.2, filed on September 19, 2023, entitled "A bio-organic fertilizer produced using by-products of the sugar industry and its preparation method". Technical Field

[0002] This invention belongs to the field of bio-organic fertilizer technology, specifically relating to the application of bio-organic fertilizer produced using sugar industry by-products in improving tomato yield and / or quality. Background Technology

[0003] Water-soluble fertilizers are multi-nutrient fertilizers that are completely or partially soluble in water. Their aqueous solutions or suspensions allow for spraying and drip irrigation, achieving integrated water and fertilizer management. They are more easily absorbed by plants and have higher absorption efficiency. The bio-organic fertilizers in water-soluble fertilizers are refined agricultural production materials that utilize organic resources such as free amino acids, mineral-derived humic acid, seaweed extracts, chitosan, polyglutamic acid, polyaspartic acid, molasses, low-value fish, and fermentation degradation products. The naturally degradable components they contain have biostimulatory effects and play a scientifically important role in the ecological systems of soil, plants, and microorganisms, as well as the sustainable development of the sugar industry.

[0004] The sugar industry generates numerous wastes, such as bagasse, sugar filter mud, and alcoholic beverage waste. How to treat these wastes is a key concern for the industry's development. Currently, sugar filter mud is treated through solid composting. However, solid composting involves long fermentation times, produces odors that affect the air quality, and has low fertilizer efficiency, resulting in unsatisfactory effects on plants. Therefore, developing fertilizers that better utilize sugar filter mud is one direction for solving the problem of solid composting of sugar filter mud. Developing bio-organic fertilizers that are more easily absorbed by plants is a pressing issue that needs to be addressed. Summary of the Invention

[0005] In view of this, the present invention aims to provide an application of bio-organic fertilizer produced using byproducts of the sugar industry in improving tomato yield and / or quality.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides an application of bio-organic fertilizer produced using byproducts of the sugar industry in improving tomato yield and / or quality; The bio-organic fertilizer produced using byproducts of the sugar industry comprises, by weight, 30-40 parts water-soluble calcium humate, 20-35 parts filter mud fermentation products, 15-25 parts magnesium nitrate hexahydrate, 0.4-1 part boric acid, 0.4-1 part zinc sulfate, 1-2 parts sodium molybdate, 1-2 parts amino acids, 1-2 parts sodium diisooctyl succinate sulfonate, 0.5-1 part D-glucuronic acid, and 1-2 parts sodium polyacrylate.

[0007] Preferably, the water-soluble calcium humate contains ≥15% humic acid, ≥15% fulvic acid, ≥4% NPK, and ≥40% organic calcium.

[0008] Preferably, the filter mud fermentation product is a sugar filter mud fermentation product, which is prepared as follows: By weight percentage, take 20-30% sugar filter mud, 3-5% yeast fermentation waste liquid, 0.2-0.3% soybean meal powder, 0.5-1% beet molasses, 0.1-0.5% urea, 0.1-0.2% potassium dihydrogen phosphate, 0.2-0.6% sodium chloride, 0.3-0.5% ferrous sulfate, and the remainder is water, and adjust the pH to 7.0-7.5; then add 3-4% by weight of compound Bacillus, and ferment for 5-7 days at 30-40℃, an aeration rate of 0.8-1.0 v / v·min, and a stirring speed of 180-200 rpm. After fermentation, separate the solid and liquid, and concentrate the liquid 5-6 times to obtain the filter mud fermentation product.

[0009] Preferably, the yeast fermentation waste liquid has the following parameters: total nitrogen content 26.1 g / L, phosphorus content 13.8 g / L, potassium content 44.5 g / L, organic matter content 254 g / L, calcium content 14.1 g / L, sulfur content 11.6 g / L, iron content 11.2 g / L, zinc content 1.2 g / L, boron content 1.4 g / L, pH 6.74, and density 1.23 g / mL.

[0010] Preferably, the compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, and Bacillus mucilaginosus in a mass ratio of 1:(0.2-0.3):(0.2-0.3):(0.4-0.6).

[0011] Preferably, the amino acid is one or any combination of polyaspartic acid, hydroxyproline, glutamic acid, and phenylalanine.

[0012] Preferably, the method for preparing the bio-organic fertilizer includes the following steps: A. Prepare the raw materials according to the proportions in claim 1; B. Take boric acid, zinc sulfate, sodium molybdate, amino acids, and sodium polyacrylate to obtain the first material, and set it aside for later use; C. Take water-soluble calcium humate, magnesium nitrate hexahydrate, sodium diisooctyl succinate sulfonate, and D-glucuronic acid, mix them, and set aside the second material; D. Mix the first material, the second material liquid, and the filter mud fermentation product, and stir for 20-30 minutes to obtain bio-organic fertilizer produced using sugar industry by-products.

[0013] Preferably, the quality includes sugar-acid ratio and color change rate.

[0014] It contains at least the following beneficial technical effects: This invention provides a bio-organic fertilizer with good water solubility. It also contains water-soluble calcium humate and magnesium nitrate, with a balanced calcium-magnesium ratio and high overall calcium and magnesium content. After dissolving in water, it has low insoluble matter content, low contamination rate, and high effective live bacteria content, making it easy to commercialize.

[0015] This invention, by adding sodium diisooctyl succinate, D-glucuronic acid, and sodium polyacrylate, improves the water solubility and permeability of the bio-organic fertilizer, thereby promoting plant absorption of the fertilizer. Spraying the bio-organic fertilizer during the pre-flowering, fruit enlargement, and color-changing stages of tomatoes can improve tomato yield, quality, and appearance to varying degrees. Detailed Implementation

[0016] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. I. Specific Implementation Methods Prepare water-soluble calcium humate with the following requirements: humic acid ≥15%, fulvic acid ≥15%, NPK ≥4%, and organic calcium ≥40%.

[0018] Example 1 Preparation of filter mud fermentation products: By weight percentage, take 20% sugar filter mud, 3% yeast fermentation waste liquid, 0.2% soybean meal powder, 0.7% beet molasses, 0.1% urea, 0.2% potassium dihydrogen phosphate, 0.2% sodium chloride, 0.3% ferrous sulfate, and the remainder is water, and adjust the pH to 7.0; then add 3% by weight of compound Bacillus to it, and ferment it for 5 days at 40℃, aeration rate of 0.8 v / v·min and stirring speed of 180 rpm. After fermentation, the solid and liquid are separated, and the liquid is concentrated 6 times to obtain the filter mud fermentation products.

[0019] The yeast fermentation waste liquid had the following parameters: total nitrogen content 26.1 g / L, phosphorus content 13.8 g / L, potassium content 44.5 g / L, organic matter content 254 g / L, calcium content 14.1 g / L, sulfur content 11.6 g / L, iron content 11.2 g / L, zinc content 1.2 g / L, boron content 1.4 g / L, pH 6.74, and density 1.23 g / mL.

[0020] The aforementioned compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, and Bacillus mucilaginosus in a mass ratio of 1:0.2:0.2:0.6.

[0021] A bio-organic fertilizer produced using byproducts of the sugar industry comprises, by weight, 30 parts water-soluble calcium humate, 25 parts filter mud fermentation products, 25 parts magnesium nitrate hexahydrate, 0.4 parts boric acid, 0.5 parts zinc sulfate, 1 part sodium molybdate, 0.8 parts hydroxyproline, 0.4 parts polyaspartic acid, 1 part sodium diisooctyl succinate sulfonate, 1 part D-glucuronic acid, and 1.4 parts sodium polyacrylate.

[0022] The method for preparing bio-organic fertilizer using sugar industry by-products as described above includes the following steps: A. Prepare the raw materials according to the above proportions; B. Take boric acid, zinc sulfate, sodium molybdate, hydroxyproline, polyaspartic acid, and sodium polyacrylate, mix them to obtain the first material, and set it aside for later use; C. Take water-soluble calcium humate, magnesium nitrate hexahydrate, sodium diisooctyl succinate sulfonate, and D-glucuronic acid, mix them, and set aside the second material; D. Mix the first material, the second material, and the filter mud fermentation product, and stir for 25 minutes to obtain bio-organic fertilizer produced using sugar industry by-products.

[0023] Example 2 Preparation of filter mud fermentation product: By weight percentage, take 23% sugar filter mud, 4% yeast fermentation waste liquid, 0.3% soybean meal powder, 0.5% beet molasses, 0.4% urea, 0.1% potassium dihydrogen phosphate, 0.5% sodium chloride, 0.4% ferrous sulfate, and the remainder is water, and adjust the pH to 7.3; then add 3% by weight of compound Bacillus to it, and ferment it for 6 days at 33℃, aeration rate of 0.9 v / v·min and stirring speed of 200 rpm. After fermentation, the solid and liquid are separated, and the liquid is concentrated 5 times to obtain the filter mud fermentation product.

[0024] The yeast fermentation waste liquid had the following parameters: total nitrogen content 26.1 g / L, phosphorus content 13.8 g / L, potassium content 44.5 g / L, organic matter content 254 g / L, calcium content 14.1 g / L, sulfur content 11.6 g / L, iron content 11.2 g / L, zinc content 1.2 g / L, boron content 1.4 g / L, pH 6.74, and density 1.23 g / mL.

[0025] The aforementioned compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, and Bacillus mucilaginosus in a mass ratio of 1:0.2:0.3:0.5.

[0026] A bio-organic fertilizer produced using byproducts of the sugar industry comprises, by weight, 34 parts water-soluble calcium humate, 35 parts filter mud fermentation products, 18 parts magnesium nitrate hexahydrate, 0.5 parts boric acid, 1 part zinc sulfate, 1.6 parts sodium molybdate, 0.5 parts polyaspartic acid, 0.5 parts phenylalanine, 1.3 parts sodium diisooctyl succinate sulfonate, 0.7 parts D-glucuronic acid, and 2 parts sodium polyacrylate.

[0027] The method for preparing bio-organic fertilizer using sugar industry by-products as described above includes the following steps: A. Prepare the raw materials according to the above proportions; B. Take boric acid, zinc sulfate, sodium molybdate, polyaspartic acid, phenylalanine, and sodium polyacrylate, mix them to obtain the first material, and set it aside for later use; C. Take water-soluble calcium humate, magnesium nitrate hexahydrate, sodium diisooctyl succinate sulfonate, and D-glucuronic acid, mix them, and set aside the second material; D. Mix the first material, the second material, and the filter mud fermentation product, and stir for 20 minutes to obtain bio-organic fertilizer produced using sugar industry by-products.

[0028] Example 3 Preparation of filter mud fermentation product: By weight percentage, take 25% sugar filter mud, 5% yeast fermentation waste liquid, 0.2% soybean meal powder, 0.8% beet molasses, 0.3% urea, 0.2% potassium dihydrogen phosphate, 0.4% sodium chloride, 0.3% ferrous sulfate, and the remainder is water, and adjust the pH to 7.2; then add 4% by weight of compound Bacillus to it, and ferment for 6 days at 36℃, aeration rate of 1.0 v / v·min and stirring speed of 190 rpm. After fermentation, the solid and liquid are separated, and the liquid is concentrated 6 times to obtain the filter mud fermentation product.

[0029] The yeast fermentation waste liquid had the following parameters: total nitrogen content 26.1 g / L, phosphorus content 13.8 g / L, potassium content 44.5 g / L, organic matter content 254 g / L, calcium content 14.1 g / L, sulfur content 11.6 g / L, iron content 11.2 g / L, zinc content 1.2 g / L, boron content 1.4 g / L, pH 6.74, and density 1.23 g / mL.

[0030] The aforementioned compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, and Bacillus mucilaginosus in a mass ratio of 1:0.3:0.3:0.4.

[0031] A bio-organic fertilizer produced using byproducts of the sugar industry comprises, by weight, 35 parts water-soluble calcium humate, 30 parts filter mud fermentation products, 20 parts magnesium nitrate hexahydrate, 0.6 parts boric acid, 0.4 parts zinc sulfate, 2 parts sodium molybdate, 0.4 parts glutamic acid, 1 part phenylalanine, 1.5 parts sodium diisooctyl succinate sulfonate, 0.5 parts D-glucuronic acid, and 1.2 parts sodium polyacrylate.

[0032] The method for preparing bio-organic fertilizer using sugar industry by-products as described above includes the following steps: A. Prepare the raw materials according to the above proportions; B. Take boric acid, zinc sulfate, sodium molybdate, glutamic acid, phenylalanine, and sodium polyacrylate, mix them, and set aside. C. Take water-soluble calcium humate, magnesium nitrate hexahydrate, sodium diisooctyl succinate sulfonate, and D-glucuronic acid, mix them, and set aside the second material; D. Mix the first material, the second material, and the filter mud fermentation product, and stir for 30 minutes to obtain bio-organic fertilizer produced using sugar industry by-products.

[0033] Example 4 Preparation of filter mud fermentation product: By weight percentage, take 30% sugar filter mud, 4% yeast fermentation waste liquid, 0.3% soybean meal powder, 1% beet molasses, 0.5% urea, 0.2% potassium dihydrogen phosphate, 0.6% sodium chloride, 0.5% ferrous sulfate, and the remainder is water, and adjust the pH to 7.5; then add 4% by weight of compound Bacillus to it, and ferment for 7 days at 30℃, aeration rate of 1.0 v / v·min and stirring speed of 200 rpm. After fermentation, the solid and liquid are separated, and the liquid is concentrated 6 times to obtain the filter mud fermentation product.

[0034] The yeast fermentation waste liquid had the following parameters: total nitrogen content 26.1 g / L, phosphorus content 13.8 g / L, potassium content 44.5 g / L, organic matter content 254 g / L, calcium content 14.1 g / L, sulfur content 11.6 g / L, iron content 11.2 g / L, zinc content 1.2 g / L, boron content 1.4 g / L, pH 6.74, and density 1.23 g / mL.

[0035] The aforementioned compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, and Bacillus mucilaginosus in a mass ratio of 1:0.3:0.3:0.4.

[0036] A bio-organic fertilizer produced using byproducts of the sugar industry comprises, by weight, 40 parts water-soluble calcium humate, 20 parts filter mud fermentation products, 15 parts magnesium nitrate hexahydrate, 1 part boric acid, 0.8 parts zinc sulfate, 1.6 parts sodium molybdate, 0.5 parts polyaspartic acid, 0.5 parts hydroxyproline, 0.5 parts glutamic acid, 0.5 parts phenylalanine, 2 parts sodium diisooctyl succinate sulfonate, 0.6 parts D-glucuronic acid, and 1 part sodium polyacrylate.

[0037] The method for preparing bio-organic fertilizer using sugar industry by-products as described above includes the following steps: A. Prepare the raw materials according to the above proportions; B. Take boric acid, zinc sulfate, sodium molybdate, polyaspartic acid, hydroxyproline, glutamic acid, phenylalanine, and sodium polyacrylate, mix them to obtain the first material, and set it aside for later use; C. Take water-soluble calcium humate, magnesium nitrate hexahydrate, sodium diisooctyl succinate sulfonate, and D-glucuronic acid, mix them, and set aside the second material; D. Mix the first material, the second material, and the filter mud fermentation product, and stir for 20 minutes to obtain bio-organic fertilizer produced using sugar industry by-products.

[0038] Comparative Example 1 The difference from Example 1 is that there is no filter mud fermentation product.

[0039] Comparative Example 2 The difference from Example 1 is that it does not contain sodium diisooctyl succinate sulfonate, D-glucuronic acid, and sodium polyacrylate.

[0040] Comparative Example 3 The difference from Example 1 is that in the preparation of the filter mud fermentation product, the compound Bacillus is composed of Bacillus subtilis and Bacillus mucilaginosus in a mass ratio of 1:0.6.

[0041] Comparative Example 4 The difference from Example 1 is that in the preparation of the filter mud fermentation product, the compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis and Bacillus mucilaginosus in a mass ratio of 1:0.2:0.6.

[0042] II. Effect Experiment 1. Performance index testing of bio-organic fertilizer The humic acid content was determined according to NY / T1971, the insoluble matter content in water was determined according to NY / T1973, the number of viable bacteria was determined according to NY / T2321-2013, and the contamination rate was determined according to NY / T2321-2013. The results are shown in Table 1.

[0043] Table 1

[0044] According to the test results in Table 1, the humic acid content is mainly affected by water-soluble calcium humate and the fermentation products of filter mud. Sodium diisooctyl succinate, D-glucuronic acid and sodium polyacrylate have no effect on the humic acid content. Based on the comparison between Comparative Examples 3 and 4 and Example 1, the inventors analyzed that the selection of compound Bacillus will have a certain impact on fermentation, mainly in the inhibition of miscellaneous bacteria, which in turn affects the humic acid content and the number of effective viable bacteria.

[0045] 2. Stability testing of bio-organic fertilizer The bio-organic fertilizer described in "I. Specific Implementation" was stored at room temperature, away from light, and in a dry environment. After 3 months and 6 months of storage, the insoluble matter and viable bacteria in the water were tested again. The results are shown in Table 2.

[0046] Table 2

[0047] As shown in Table 2, except for Comparative Example 2, the water solubility stability of the other bio-organic fertilizers is relatively stable. The inventors analyzed that the combined use of sodium diisooctyl succinate, D-glucuronic acid and sodium polyacrylate can improve the stability of bio-organic fertilizers.

[0048] 3. Apply bio-organic fertilizer to tomatoes and observe its effects on the tomatoes. An experiment was conducted in a tomato greenhouse in Gaoqing County, Shandong Province. The tomato variety was Yuanwei No. 1, and the soil was moist, light loam with medium fertility and uniform distribution. The experimental plot area was 12 m2, and the planting density was 2000 plants / mu. Bio-organic fertilizer was sprayed during the pre-flowering, fruit enlargement, and color-changing stages of the tomatoes. The spraying method was to dilute the bio-organic fertilizer with water at a ratio of 1:200 and spray it on the leaves, branches, and fruit surface. The spraying degree should be such that water droplets do not converge. Other planting requirements were treated according to conventional requirements. An equal amount of water was sprayed as a control. The changes, yield, and quality of the tomatoes were observed. The results are shown in Table 3.

[0049] Table 3

[0050] Note: Tomato ripening is judged when the ripening area of ​​the fruit reaches 80% or more.

[0051] According to the test results in Table 3, it can be seen that spraying bio-organic fertilizer during the pre-flowering stage, fruit enlargement stage, and color-changing stage of tomatoes can improve the yield, quality, and appearance of tomatoes to varying degrees. Compared with the bio-organic fertilizer in Comparative Examples 1-4, spraying the bio-organic fertilizer in Examples 1-4 can further improve the yield and quality of tomatoes, and the taste is even better. This indicates that the filter mud fermentation products, sodium diisooctyl succinate, D-glucuronic acid, and sodium polyacrylate can all improve the absorption effect of bio-organic fertilizer to varying degrees.

[0052] The above description is only a preferred embodiment of the present invention. It should be noted that those skilled in the art can make several improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. The application of a bio-organic fertilizer produced using byproducts of the sugar industry in improving tomato yield and / or quality; The bio-organic fertilizer produced using byproducts of the sugar industry comprises, by weight, 30-40 parts water-soluble calcium humate, 20-35 parts filter mud fermentation products, 15-25 parts magnesium nitrate hexahydrate, 0.4-1 part boric acid, 0.4-1 part zinc sulfate, 1-2 parts sodium molybdate, 1-2 parts amino acids, 1-2 parts sodium diisooctyl succinate sulfonate, 0.5-1 part D-glucuronic acid, and 1-2 parts sodium polyacrylate.

2. The application according to claim 1, characterized in that, The water-soluble calcium humate contains ≥15% humic acid, ≥15% fulvic acid, ≥4% NPK, and ≥40% organic calcium.

3. The application according to claim 1, characterized in that, The filter mud fermentation product is a sugar filter mud fermentation product, and its specific preparation is as follows: By weight percentage, take 20-30% sugar filter mud, 3-5% yeast fermentation waste liquid, 0.2-0.3% soybean meal powder, 0.5-1% beet molasses, 0.1-0.5% urea, 0.1-0.2% potassium dihydrogen phosphate, 0.2-0.6% sodium chloride, 0.3-0.5% ferrous sulfate, and the remainder is water, and adjust the pH to 7.0-7.5; then add 3-4% by weight of compound Bacillus, and ferment for 5-7 days at 30-40℃, an aeration rate of 0.8-1.0 v / v·min, and a stirring speed of 180-200 rpm. After fermentation, separate the solid and liquid, and concentrate the liquid 5-6 times to obtain the filter mud fermentation product.

4. The application according to claim 1, characterized in that, The yeast fermentation waste liquid had the following parameters: total nitrogen content 26.1 g / L, phosphorus content 13.8 g / L, potassium content 44.5 g / L, organic matter content 254 g / L, calcium content 14.1 g / L, sulfur content 11.6 g / L, iron content 11.2 g / L, zinc content 1.2 g / L, boron content 1.4 g / L, pH 6.74, and density 1.23 g / mL.

5. The application according to claim 1, characterized in that, The compound Bacillus is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, and Bacillus mucilaginosus in a mass ratio of 1:(0.2-0.3):(0.2-0.3):(0.4-0.6).

6. The application according to claim 1, characterized in that, The amino acid is one or any combination of polyaspartic acid, hydroxyproline, glutamic acid, and phenylalanine.

7. The application according to claim 1, characterized in that, The method for preparing the bio-organic fertilizer includes the following steps: A. Prepare the raw materials according to the proportions in claim 1; B. Take boric acid, zinc sulfate, sodium molybdate, amino acids, and sodium polyacrylate to obtain the first material, and set it aside for later use; C. Take water-soluble calcium humate, magnesium nitrate hexahydrate, sodium diisooctyl succinate sulfonate, and D-glucuronic acid, mix them, and set aside the second material; D. Mix the first material, the second material liquid, and the filter mud fermentation product, and stir for 20-30 minutes to obtain bio-organic fertilizer produced using sugar industry by-products.

8. The application according to claim 1, characterized in that, The quality parameters include the sugar-acid ratio and the color change rate.

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