A cell displaying silipptide r5 on the surface, and a preparation method and application thereof

CN122168490APending Publication Date: 2026-06-09SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Filing Date
2024-12-06
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing biological building materials have low mechanical strength and high cost, industrial anaerobic fermentation processes are complex and costly, and the display of diatom peptide R5 on the surface of E. coli has not been effectively applied.

Method used

By displaying diatom peptide R5 on the surface of Escherichia coli, gene expression was carried out using the TertR system induced by dehydrated tetracycline, and the mixture was mixed with silica material to form bio-bricks and core-shell structures, thus constructing an anaerobic microenvironment.

Benefits of technology

This has improved the mechanical strength of bio-bricks, simplified fermentation equipment and production conditions, and increased the yield of anaerobic fermentation, especially the yield of butanol and hydrogen.

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Abstract

The application discloses a cell for displaying diatom peptide R5 on the surface, and a preparation method and application thereof. The cell for displaying diatom peptide R5 on the surface is mixed with silica-containing sandstone material, so that the preparation and regeneration of a biological building material are realized. The cell for displaying R5 peptide on the surface is in-situ mineralized and self-assembled into an anaerobic core-shell structure, an anaerobic microenvironment is created, and the fermentation yield under anaerobic conditions is improved.
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