A nanoscale enzyme chromatography reagent cassette

Abstract

The application relates to the technical field of reagent cassettes, and specifically provides a nanometer enzyme chromatographic reagent cassette, which comprises a cassette main body, notches are formed in the two sides of the cassette main body, clamping seats are fixedly installed on the upper surfaces of the two sides of the cassette main body and close to the edges, a cover assembly is arranged on the upper side of the outer side of the cassette main body, a storage plate is fixedly installed on the inner side of the upper side of the cassette main body, channels are formed in the two sides of the inside of the storage plate, a frame is fixedly installed in the middle of the inside of the cassette main body, sliding racks are slidingly installed at positions corresponding to the channels of the frame, suction assemblies are arranged in the two sliding racks, and a shell is fixedly installed on the inner side of the middle of the storage plate. The application can automatically mix buffer solution and sampling samples, can mix and prepare a chromogenic solution, and solves the problems of complex operation and low detection precision in nanometer enzyme immune detection by using a reagent cassette.

Description

Technical Field

[0001] This invention relates to the field of nanozyme cartridge technology, and specifically provides a cartridge for nanozyme chromatography reagents. Background Technology

[0002] In immunochromatographic assays, the detection is primarily performed on a reagent cartridge. Existing cartridges contain chromatographic test strips, with a sample well and a colorimetric window at the top. During immunoassay, the chromogenic solution is added to the colorimetric window, and the sample to be tested is added to the sample well. The sample reacts with the chromogenic solution to obtain the detection result. Currently, the accuracy of the detection results is significantly related to the quality of the chromogenic solution. Taking nanozyme immunochromatography as an example, the chromogenic solution includes solution A and solution B. Solution A typically consists of PBS buffer, citric acid, EDTA (ethylenediaminetetraacetic acid), ProcLin-300, and hydrogen peroxide. Solution B typically consists of PBS buffer, citric acid, EDTA, ProcLin-300, sodium thiosulfate, etc. To improve detection accuracy, solutions A and B are usually prepared manually and on-demand to obtain the chromogenic solution. Because the weight ratio of solution A and solution B is required during testing, it is not easy to accurately control the amount of solution A and solution B when preparing the colorimetric solution manually, which may directly affect the accuracy of the test results. Therefore, we need to improve this aspect. Summary of the Invention

[0003] The purpose of this invention is to solve the problems existing in the background art by proposing a nanozyme chromatography reagent cartridge.

[0004] To achieve the above objectives, the present invention provides a cartridge for nanozyme chromatography reagents, comprising: a cartridge body, slots on both sides of the cartridge body, card holders fixedly installed on the upper surfaces of both sides of the cartridge body near the edges, a capping assembly on the upper outer side of the cartridge body, a shelf fixedly installed on the upper inner side of the cartridge body, channels on both sides of the shelf, a frame fixedly installed in the middle of the interior of the cartridge body, slide brackets slidably installed on the frame and at positions corresponding to the channels, suction assemblies inside the two slide brackets, a shell fixedly installed in the middle of the inner side of the shelf, a mixing assembly inside the shell, a fixing seat fixedly installed at the bottom of the inner side of the shell, an inner shell fixedly installed on the upper surface of the fixing seat, and reagent detection boxes on the upper surface of the shelf and near each corner.

[0005] Preferably, the sealing assembly includes a card window, and buckles adapted to the card mounting seat are installed on both sides of the card window.

[0006] Preferably, the suction assembly includes a miniature water pump fixedly installed inside the slide, with a flexible hose connected to the bottom end of the miniature water pump, and a rigid pipe connected to the end of the flexible hose away from the miniature water pump, the end of the rigid pipe penetrating the inside of the housing and communicating with the inner shell.

[0007] Preferably, a delivery pipe is connected and installed above the end of the micro water pump away from the hose, and drip tubes are respectively connected and installed at both ends inside the delivery pipe.

[0008] Preferably, the mixing assembly includes a fixing ring rotatably mounted on the upper inner side of the housing. Solvent tubes are provided at both ends of the inner side of the fixing ring. A connecting seat is installed between the two solvent tubes. A driving assembly is provided on the upper inner side of the connecting seat. Connecting pipes are installed on the lower inner sides of the two solvent tubes. Three dispersing pipes are installed on the inner side of the connecting pipes. The three dispersing pipes are arranged in a semi-arc array. Caps are threadedly connected to the upper outer sides of the two solvent tubes.

[0009] Preferably, the drive assembly includes a rotating shaft that is installed through the inside of the connecting seat, and a micro motor is fixedly mounted on the upper end of the rotating shaft. The micro motor is fixedly mounted on the top wall of the frame.

[0010] Preferably, cylinders are fixedly installed on both sides of the outer wall of the slide, and connecting plates are fixedly installed on the telescopic ends of the two cylinders. Connecting rods are fixedly installed on the lower inner side of the two connecting plates. Rollers are rotatably fitted on the outer side of the two connecting rods, and one end of the rollers abuts against the outer side of the reagent test box. Connecting blocks are fixedly installed on the bottom of the two connecting rods, and dust collection boxes are fixedly installed on the bottom of the two connecting blocks. Multiple nozzles are installed in communication between the two sides of the interior of the two dust collection boxes.

[0011] Preferably, both sets of dust collection boxes are connected to an air pump via interconnected air supply pipes. The air pump is fixedly connected to the slide, and a battery is installed on one side of the inner wall of the slide.

[0012] Compared with the prior art, the present invention has the following beneficial effects: By setting a fixing ring and a connecting seat inside the inner shell, the solution inside the solvent tube can be automatically fed and mixed when the micro motor drives the rotating shaft to operate. The liquid in the solvent tube can be transported to the inner shell through the connecting pipe. The rotating solvent tube can automatically mix the two sets of solvents discharged from the inner shell through the dispensing pipe at the connecting pipe. This avoids the trouble of manually mixing the solution by repeatedly drawing it with a dropper in the existing technology.

[0013] By setting a frame inside the main body of the cartridge, the corresponding slide moves on both sides of the frame. The miniature water pump and dropper inside the slide can draw the mixed reagent inside the inner shell, so that the two mixed reagents can be automatically combined. Then, the dropper can drip the reagent into the dropper opening of the reagent test box, thereby rapidly improving the detection efficiency of the reagent. This operation can detect four sets of reagents at the same time, which is efficient and convenient.

[0014] The movement of the carriage can cause the cylinder and dust collection box at the front to move. In order to ensure that the dropper accurately drips onto the drip port of the reagent test box, the roller can press against one side of the reagent test box to ensure that the position of the reagent test box can be more stable during the test. If any dripped solvent is placed on the surface of the plate, the air pump can blow the excess solvent out from both ends of the slot through the dust collection box. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram showing the overall structure of the present invention disassembled and connected. Figure 3 This is a schematic diagram of the connection of the inner structural part of the card box body of the present invention; Figure 4 This is a schematic diagram of the connection of the inner part of the housing structure of the present invention; Figure 5 This is a schematic diagram of the connection structure between the housing and the micro water pump of the present invention; Figure 6 This is a schematic diagram showing the connection between the dust collector box and the cylinder of the present invention.

[0016] In the diagram: 1. Card box body; 2. Card window; 3. Frame; 4. Card seat; 5. Buckle; 6. Shelf; 7. Reagent test box; 8. Channel; 9. Dropper; 10. Fixing seat; 11. Shell; 12. Connecting tube; 13. Rigid tube; 14. Micro motor; 15. Slide; 16. Solvent tube; 17. Fixing ring; 18. Connecting seat; 19. Dispensing tube; 20. Cap; 21. Shaft; 22. Inner shell; 23. Flexible tube; 24. Delivery tube; 25. Battery; 26. Micro water pump; 27. Cylinder; 28. Connecting plate; 29. ​​Roller; 30. Dust collection box; 31. Nozzle; 32. Connecting rod; 33. Connecting block; 34. Air pump; 35. Slot. Detailed Implementation

[0017] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] Numerous specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and therefore the invention is not limited to the specific embodiments disclosed below.

[0019] like Figures 1-6 A nanozyme chromatography reagent cartridge is shown, comprising: a cartridge body 1, slots 35 on both sides of the cartridge body 1, card holders 4 fixedly installed on the upper surface of both sides of the cartridge body 1 near the edge, a capping assembly on the upper outer side of the cartridge body 1, a shelf 6 fixedly installed on the upper inner side of the cartridge body 1, channels 8 on both sides of the shelf 6, a frame 3 fixedly installed in the middle of the interior of the cartridge body 1, slides 15 slidably installed on the frame 3 at positions corresponding to the channels 8, suction assemblies inside the two slides 15, a shell 11 fixedly installed in the middle of the inner side of the shelf 6, a mixing assembly inside the shell 11, a fixing seat 10 fixedly installed at the bottom of the inner side of the shell 11, the shell 11 is stably positioned inside the cartridge body 1 with the fixing seat 10 connected, an inner shell 22 fixedly installed on the upper surface of the fixing seat 10, and reagent detection boxes 7 are provided on the upper surface of the shelf 6 near each corner.

[0020] The sealing assembly includes a card window 2, and buckles 5 that are adapted to card holders 4 are installed on both sides of the card window 2. The movable snaps of the buckles 5 and the card holders 4 can install the card window 2 on the upper outer side of the card box body 1.

[0021] The suction assembly includes a miniature water pump 26 fixedly installed inside the slide 15. A flexible tube 23 is connected to the bottom of the miniature water pump 26. A rigid tube 13 is connected to the end of the flexible tube 23 away from the miniature water pump 26. The end of the rigid tube 13 passes through the inside of the housing 11 and is connected to the inner housing 22. A delivery tube 24 is connected to the top of the end of the miniature water pump 26 away from the flexible tube 23. Droppers 9 are connected to both ends of the delivery tube 24. The miniature water pump 26 can draw the solvent from the inner housing 22 through the suction of the flexible tube 23 and the rigid tube 13.

[0022] To enable more precise liquid control, the dropper 9 can be equipped with an internal electromagnetic valve, depending on the application requirements.

[0023] The mixing assembly includes a fixing ring 17 rotatably mounted on the upper inner side of the housing 11. Solvent tubes 16 are provided at both ends of the inner side of the fixing ring 17. A connecting seat 18 is installed between the two solvent tubes 16. A driving assembly is provided on the upper inner side of the connecting seat 18. A connecting pipe 12 is installed on the lower inner side of the two solvent tubes 16. Three dispersing pipes 19 are installed on the inner side of the connecting pipe 12. The three dispersing pipes 19 are arranged in a semi-arc array. Caps 20 are threadedly connected to the upper outer side of the two solvent tubes 16.

[0024] The drive assembly includes a rotating shaft 21 that runs through the inside of the connector 18. A micro motor 14 is fixedly mounted on the upper end of the rotating shaft 21. The micro motor 14 is fixedly mounted on the top wall of the frame 3. The micro motor 14 can drive the rotating shaft 21 to move, thereby causing the fixing ring 17, solvent tube 16, and connecting tube 12 to rotate. A control valve can be added inside the connecting tube 12 to drain the liquid. Then, the dispersing tube 19 drains the solution inside the corresponding solvent tube 16 into the inner shell 22. During the rotation, the two sets of solvents are mixed.

[0025] Cylinders 27 are fixedly installed on both sides of the outer wall of the slide 15. Connecting plates 28 are fixedly installed on the telescopic ends of the two cylinders 27. Connecting rods 32 are fixedly installed on the lower inner side of the two connecting plates 28. Rollers 29 are rotatably mounted on the outer side of the two connecting rods 32, and one end of the rollers 29 abuts against the outer side of the reagent test box 7. The cylinders 27 can drive the connecting plates 28, the connecting plates 28, and the rollers 29 to move to one side of the reagent test box 7 until the rollers 29 abut against the outer side of the reagent test box 7. Connecting blocks 33 are fixedly installed at the bottom of the two connecting rods 32. Dust collection boxes 30 are fixedly installed at the bottom of the two connecting blocks 33. Multiple nozzles 31 are installed inside the two dust collection boxes 30 on both sides.

[0026] Both dust collection boxes 30 are connected to an air pump 34 via air supply pipes. The air pump 34 is fixedly connected to the slide 15. A battery 25 is installed on one side of the inner wall of the slide 15. The air pump 34 can be connected to the inside of the dust collection box 30 through the air supply pipes at both ends. The gas is then sprayed onto the surface of the shelf 6 through the nozzle 31 of the dust collection box 30.

[0027] Battery 25 can power the micro motor 14, micro water pump 26, and air pump 34.

[0028] Working principle: In use, the reagent test boxes 7 are placed at the corners of the shelf 6. The corresponding slides 15 can move to the positions of the two reagent test boxes 7 on the same side, so that the dropper 9 corresponds to the dripping port of the reagent test box 7. After the micro motor 14 is powered on, it drives the rotating shaft 21 to rotate. The rotation of the rotating shaft 21 drives the connecting seat 18 and the two solvent tubes 16 to rotate. The connecting tube 12 will rotate synchronously with the solvent tubes 16. The connecting tube 12 can discharge the corresponding solvent through the dispensing tube 19 into the inner shell 22. During the rotation, the two sets of solvents are mixed. Then, through the suction of the micro water pump 26, the rigid tube 13 and the flexible tube 23 transport the solvent to the upper delivery tube 24, and then divert it to the corresponding dropper 9, which automatically falls into the dripping port of the corresponding reagent test box 7. The solvent is observed through the display window at the reagent test box 7. This method can quickly improve the detection efficiency of the reagent, and this operation can detect four sets of reagents at the same time, which is efficient and convenient. In order to ensure that the dropper 9 does not waste solvent, the position between the dropper 9 and the reagent test box 7 needs to be maintained when dispensing. The movement of the slide 15 can cause the front cylinder 27 and the dust collection box 30 to move. Under the extension and retraction drive of the cylinder 27, the roller 29 moves to one side of the reagent test box 7 until the roller 29 abuts against one side of the reagent test box 7, so as to ensure that the position of the reagent test box 7 corresponds to the position of the dropper 9 during detection. When the reagent test box 7 is removed later, if any dripped solvent is placed on the surface of the shelf 6, the air pump 34 can blow the excess solvent out from both ends of the slot 35 through the dust collection box 30 and multiple sets of nozzles 31.

[0029] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention. The scope of protection claimed by the appended claims and their equivalents is defined.

Claims

1. A cartridge for nanozyme chromatography reagents, comprising: The card box body (1) is characterized in that: slots (35) are provided on both sides of the card box body (1), card seats (4) are fixedly installed on the upper surface of both sides of the card box body (1) and near the edge, a capping assembly is provided on the upper outer side of the card box body (1), a shelf (6) is fixedly installed on the upper inner side of the card box body (1), channels (8) are provided on both sides of the shelf (6), a frame (3) is fixedly installed in the middle of the inside of the card box body (1), a slide (15) is slidably installed on the frame (3) and at the position corresponding to the channel (8), a suction assembly is provided inside the two slides (15), a shell (11) is fixedly installed in the middle of the inner side of the shelf (6), a mixing assembly is provided inside the shell (11), a fixing seat (10) is fixedly installed at the bottom of the inner side of the shell (11), an inner shell (22) is fixedly installed on the upper surface of the fixing seat (10), and a reagent detection box (7) is provided on the upper surface of the shelf (6) and near each corner.

2. The nanozyme chromatography reagent cartridge according to claim 1, characterized in that: The cover assembly includes a card window (2), and buckles (5) that are adapted to the card mounting base (4) are installed on both sides of the card window (2).

3. The nanozyme chromatography reagent cartridge according to claim 1, characterized in that: The suction assembly includes a miniature water pump (26) fixedly installed inside the slide (15). The bottom end of the miniature water pump (26) is connected to a hose (23). The end of the hose (23) away from the miniature water pump (26) is connected to a rigid pipe (13). The end of the rigid pipe (13) penetrates the inside of the housing (11) and is connected to the inner shell (22).

4. The nanozyme chromatography reagent cartridge according to claim 3, characterized in that: The micro water pump (26) is connected to a delivery pipe (24) above the end away from the hose (23), and the two ends of the delivery pipe (24) are connected to drip tubes (9).

5. The nanozyme chromatography reagent cartridge according to claim 1, characterized in that: The mixing assembly includes a fixing ring (17) rotatably mounted on the upper inner side of the housing (11). Solvent tubes (16) are provided at both ends of the inner side of the fixing ring (17). A connecting seat (18) is installed between the two solvent tubes (16). A driving assembly is provided on the upper inner side of the connecting seat (18). A connecting pipe (12) is connected to the lower inner side of the two solvent tubes (16). Three dispersing pipes (19) are connected to the inner side of the connecting pipe (12). The three dispersing pipes (19) are arranged in a semi-arc array. A cap (20) is threadedly connected to the upper outer side of the two solvent tubes (16).

6. The nanozyme chromatography reagent cartridge according to claim 5, characterized in that: The drive assembly includes a rotating shaft (21) that runs through the inside of the connecting seat (18). A micro motor (14) is fixedly installed at the upper end of the rotating shaft (21). The micro motor (14) is fixedly installed at the top wall of the frame (3).

7. The nanozyme chromatography reagent cartridge according to claim 1, characterized in that: Cylinders (27) are fixedly installed on both sides of the outer wall of the slide (15). Connecting plates (28) are fixedly installed on the telescopic ends of the two cylinders (27). Connecting rods (32) are fixedly installed on the lower inner side of the two connecting plates (28). Rollers (29) are rotatably mounted on the outer side of the two connecting rods (32), and one end of the rollers (29) abuts against the outer side of the reagent test box (7). Connecting blocks (33) are fixedly installed at the bottom of the two connecting rods (32). Dust collection boxes (30) are fixedly installed at the bottom of the two connecting blocks (33). Multiple nozzles (31) are connected to both sides inside the two sets of dust collection boxes (30).

8. The nanozyme chromatography reagent cartridge according to claim 7, characterized in that: Both sets of dust collection boxes (30) are connected to an air pump (34) through a connected air supply pipe. The air pump (34) is fixedly connected to the slide (15). A battery (25) is installed on one side of the inner wall of the slide (15).

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