Nucleic acid extractor

Abstract

The utility model discloses a nucleic acid extraction instrument which comprises an instrument box body, a sample sub-packaging box is placed in the instrument box body, a magnetic bar module is arranged above the sample sub-packaging box, an XY-axis moving module is arranged at the bottom in the instrument box body, the XY-axis moving module bears the sample sub-packaging box, and the magnetic bar module is arranged above the sample sub-packaging box. A Z-axis moving module and a Z-axis moving motor are arranged on the back face of the instrument box body, a sliding block of the Z-axis moving module is connected with a magnetic bar module frame, and the magnetic bar module frame bears a magnetic bar module. According to the nucleic acid extraction device, nucleic acid extraction of a large number of samples can be carried out simultaneously, the translation and lifting structure of the samples and the magnetic bar is simple, the control program is concise, the magnetic bar enters the magnetic bar sleeve when descending, direct contact between the magnetic bar and sample liquid is avoided, and the magnetic bar and the magnetic bar sleeve can be disassembled more conveniently and simply.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction equipment, in particular to a nucleic acid extraction instrument. Background Art

[0002] In almost every laboratory, separation and purification of biomolecules is very important and indispensable. However, it is still quite difficult to purify multiple samples. Not only does it require the selection of appropriate purification technology, but the workload is also particularly large, and it is difficult to meet the current rapid development of the demand for high-throughput sample extraction and purification. Although the current nucleic acid extraction instrument can extract 96 nucleic acid samples, the internal structure is relatively complex, especially the lifting and translation of the magnetic rod and the sample part. The lifting and translation structure is complex and there are many procedures to control.

[0003] Therefore, in order to correct the above-mentioned defects, we proposed a nucleic acid extraction instrument. Utility Model Content

[0004] The utility model solves the technical problem and provides a nucleic acid extraction instrument.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a nucleic acid extraction instrument, comprising an instrument case, a sample packaging box is placed in the instrument case, a magnetic rod module is provided above the sample packaging box, an XY-axis moving module is provided at the bottom of the instrument case, the XY-axis moving module carries the sample packaging box, a Z-axis moving module and a Z-axis motion motor are provided on the back of the instrument case, a slider of the Z-axis moving module is connected to a magnetic rod module frame, and the magnetic rod module frame carries the magnetic rod module.

[0006] Furthermore, the Z-axis moving module and the Z-axis motion motor each have two, the two Z-axis moving modules are arranged in parallel, and a magnetic rod sleeve module frame is installed on the slider of the other Z-axis moving module, and a plurality of magnetic rod sleeve modules are installed on the magnetic rod sleeve module frame. The magnetic rod sleeve module frame is directly below the magnetic rod module frame, and the two have the same shape.

[0007] Furthermore, the magnetic rod module frame is in a "concave" shape, and has several mounting holes for clamping the magnetic rods. The magnetic rod sleeve module frame is in a "concave" shape, and has several clamping holes for clamping the magnetic rod sleeve. The magnetic rods and the magnetic rod sleeves correspond one to one.

[0008] Furthermore, an industrial camera is provided on the back of the instrument case.

[0009] Furthermore, an exhaust fan and an exhaust pipe connected to the exhaust fan are installed on the top of the back side of the instrument case, and the inlet end of the exhaust pipe is located in the instrument case and directly above the sample packaging box.

[0010] Furthermore, the upper end surface of the magnetic rod module frame is provided with a waist groove, and the magnetic rod module frame is longitudinally provided with a T-shaped groove from the front end surface to the rear end surface, the T-shaped groove is movably connected with a T-shaped bar, the mounting hole is located on the T-shaped bar, and the waist groove is interconnected with the T-shaped groove.

[0011] Furthermore, the upper end surface of the magnetic rod sleeve mold assembly frame is provided with a waist groove 2, and the magnetic rod sleeve mold assembly frame is longitudinally provided with a T-shaped groove 2 from the front end surface to the rear end surface. The T-shaped groove 2 is movably connected with a T-shaped bar 2, the mounting hole is located on the T-shaped bar 2, and the waist groove 2 is interconnected with the T-shaped groove 2.

[0012] Compared with the prior art, the beneficial effects of the utility model are: the device can extract nucleic acid from a large number of samples at the same time, the translation and lifting structure of the samples and the magnetic rod is simple when extracting nucleic acid from a large number of samples, the control program is concise, and the magnetic rod enters the magnetic rod cover when it descends to avoid direct contact between the magnetic rod and the sample liquid, and it is more convenient and simple to disassemble the magnetic rod and the magnetic rod cover. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the utility model from the first perspective;

[0014] Figure 2 This is a schematic diagram of the second viewing angle of the utility model;

[0015] Figure 3 This is a schematic diagram of the internal structure of the utility model;

[0016] Figure 4 This is a schematic diagram of the structure of the magnetic rod module frame in the utility model;

[0017] Figure 5 It is a schematic diagram of the structure of the magnetic rod sleeve module frame in the utility model.

[0018] Numbers in the figure:

[0019] 1. Instrument case; 2. XY axis moving module; 3. Sample packaging box; 4. Magnetic rod module rack; 5. Magnetic rod module; 6. Z axis moving module; 7. Z axis motion motor; 8. Industrial camera; 9. Exhaust fan; 10. Exhaust pipe; 11. Magnetic rod sleeve module rack; 12. Magnetic rod sleeve module; 13. T-slot 1; 14. T-bar 1; 15. T-slot 2; 16. T-bar 2. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] The utility model provides a technical solution:

[0022] See also Figure 1-5 A nucleic acid extraction instrument mainly includes an instrument box 1, which has a set of XY axis moving modules 2, two sets of Z axis moving modules 6 and a Z axis motion motor 7. The XY axis moving module 2 carries a sample packaging box 3 for left and right and front and back translation. The slider of one Z axis moving module 6 in the two sets of Z axis moving modules 6 drives a magnetic rod module frame 4 and a magnetic rod module 5 on the magnetic rod module frame 4 to rise and fall. The slider of the other Z axis moving module 6 drives a magnetic rod sleeve module frame 11 and a plurality of magnetic rod sleeve modules 12 on the magnetic rod sleeve module frame 11 to rise and fall.

[0023] Specifically, the magnetic bar module 5 includes 32 magnetic bars. Samples and magnetic beads are added to the lysis solution (containing proteinase K) in each cell of the sample dispensing box 3 in turn, and are heated and vibrated. After the sample is lysed, the sample dispensing box 3 is placed on the XY axis moving module 2, and two sets of Z axis moving modules 6 and Z axis motion motor 7 are started. The magnetic bar module frame 4 is first lowered, so that each magnetic bar enters the corresponding magnetic bar sleeve, and then the magnetic bar module frame 4 and the magnetic bar module frame 4 are controlled to descend synchronously, and the magnetic bars and magnetic bar sleeves enter the sample, and the nucleic acid is released from the sample and adsorbed. Attach to the surface of the magnetic beads, use the magnetic rod and the magnetic rod cover to transfer the magnetic beads and wash them thoroughly to obtain pure nucleic acid. In this process, the released waste gas is sucked away by the exhaust fan 9. When the next batch of sample nucleic acid in the sample distribution box 3 is extracted, the industrial camera 8 identifies the entry and exit of the magnetic rod, and then controls the reset of the two sets of Z-axis moving modules 6. The XY-axis moving module 2 drives the sample distribution box 3 to move left and right and front and back, so that the part of the sample distribution box 3 to be detected corresponds to the part directly below the magnetic rod, and then controls the magnetic rod and the magnetic rod cover to descend, and proceeds in sequence.

[0024] Among them, the magnetic rod module frame 4 and the magnetic rod sleeve module frame 11 are both in a "concave" shape, and the two raised parts of the magnetic rod module frame 4 are each provided with two rows of magnetic rods. The concave part of the magnetic rod module frame 4 allows a certain spacing between the two rows on the left and the two rows on the right, with 8 in each row, so that one magnetic rod module frame 4 has 32 magnetic rods. Similarly, the two raised parts of the magnetic rod sleeve module frame 11 are each provided with two rows of magnetic rod sleeves, with 8 in each row, so that one magnetic rod sleeve module frame 11 has 32 magnetic rod sleeves, so that extraction operations can be performed on two sample packaging boxes 3 at the same time.

[0025] A waist groove 1 is provided on the upper end surface of the magnetic rod module frame 4, and a T-slot 13 is longitudinally opened on the magnetic rod module frame 4 from the front end to the rear end surface, and the T-slot 13 is movably connected with a T-bar 14, and the mounting hole is on the T-bar 14, and the waist groove 1 is interconnected with the T-slot 13. A waist groove 2 is provided on the upper end surface of the magnetic rod sleeve module frame 11, and a T-slot 2 15 is longitudinally opened on the magnetic rod sleeve module frame 11 from the front end to the rear end surface, and the T-slot 2 15 is movably connected with a T-bar 2 16, and the mounting hole is on the T-bar 2 16, and the waist groove 2 is interconnected with the T-slot 2 14. In the later stage, the magnetic rod and the magnetic rod sleeve can be quickly removed. When removing, only the corresponding T-bar 14 and T-bar 2 16 need to be extracted, and the installation is also convenient.

[0026] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid extraction instrument, comprising an instrument case (1), wherein a sample dispensing box (3) is placed in the instrument case (1), and a magnetic bar module (5) is provided above the sample dispensing box (3), characterized in that: The bottom of the instrument case (1) is provided with an XY-axis moving module (2), the XY-axis moving module (2) carries a sample packaging box (3), the back of the instrument case (1) is provided with a Z-axis moving module (6) and a Z-axis motion motor (7), the slider of the Z-axis moving module (6) is connected to a magnetic rod module frame (4), and the magnetic rod module frame (4) carries a magnetic rod module (5).

2. The nucleic acid extractor according to claim 1, characterized in that: The Z-axis moving modules (6) and the Z-axis motion motor (7) are each provided with two Z-axis moving modules (6), the two Z-axis moving modules (6) being arranged in parallel, a magnetic rod sleeve module frame (11) being installed on the slider of the other Z-axis moving module (6), a plurality of magnetic rod sleeve modules (12) being installed on the magnetic rod sleeve module frame (11), the magnetic rod sleeve module frame (11) being located directly below the magnetic rod module frame (4), and the two have the same shape.

3. The nucleic acid extractor according to claim 2, characterized in that: The magnetic rod module frame (4) is in a "concave" shape, and has a plurality of mounting holes for clamping the magnetic rods. The magnetic rod sleeve module frame (11) is in a "concave" shape, and has a plurality of clamping holes for clamping the magnetic rod sleeves. The magnetic rods correspond to the magnetic rod sleeves one by one.

4. The nucleic acid extractor according to claim 1, characterized in that: The back of the instrument case (1) is provided with an industrial camera (8).

5. The nucleic acid extractor according to claim 1, characterized in that: An exhaust fan (9) and an exhaust pipe (10) connected to the exhaust fan (9) are installed on the top of the back of the instrument case (1); the inlet end of the exhaust pipe (10) is located inside the instrument case (1) and directly above the sample packaging box (3).

6. The nucleic acid extraction instrument according to claim 3, characterized in that: The upper end surface of the magnetic rod module frame (4) is provided with a waist groove one, and the magnetic rod module frame (4) is longitudinally provided with a T-shaped groove one (13) from the front end surface to the rear end surface, the T-shaped groove one (13) is movably connected with a T-shaped bar one (14), the mounting hole is located on the T-shaped bar one (14), and the waist groove one and the T-shaped groove one (13) are interconnected.

7. The nucleic acid extraction instrument according to claim 3, characterized in that: The upper end surface of the magnetic rod sleeve mold assembly frame (11) is provided with a waist groove II, and the magnetic rod sleeve mold assembly frame (11) is longitudinally provided with a T-shaped groove II (15) from the front end surface to the rear end surface, the T-shaped groove II (15) is movably connected with a T-shaped bar II (16), the mounting hole is located on the T-shaped bar II (16), and the waist groove II and the T-shaped groove II (15) are interconnected.

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