Reagent device for detecting EGFR (epidermal growth factor receptor) gene hotspot mutation

Abstract

The utility model discloses a reagent device for detecting EGFR (epidermal growth factor receptor) gene hotspot mutation, which comprises a reagent storage shell, arc-shaped positioning grooves distributed in a linear array are arranged on the inner bottom wall of the reagent storage shell, fixing blocks distributed in a linear array are fixedly connected to the inner walls of two sides of the reagent storage shell, through holes are arranged on the upper surfaces of the fixing blocks, and the arc-shaped positioning grooves are communicated with the through holes. The upper surface of the fixing block is fixedly connected with an annular sliding rail, the outer surface of the annular sliding rail is slidably connected with a rotating ring in a sleeving mode, and the outer surface of the rotating ring is provided with anti-skid grooves distributed in an annular array. And the practicability is improved.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a reagent device for detecting hotspot mutations of EGFR genes. Background Art

[0002] EGFR is the expression product of the proto-oncogene c-erbB1 and a member of the epidermal growth factor receptor family, which includes HER1 (erbB1, EGFR), HER2 (erbB2, NEU), HER3 (erbB3) and HER4 (erbB4). The ER family plays an important regulatory role in cell physiological processes. EGFR is widely distributed on the cell surfaces of mammalian epithelial cells, fibroblasts, glial cells, keratinocytes, etc. The EGFR signaling pathway plays an important role in physiological processes such as cell growth, proliferation and differentiation. The loss of function of protein tyrosine kinases such as EGFR or the abnormal activity of key factors in its related signaling pathways or abnormal cell localization can cause tumors, diabetes, immunodeficiency and cardiovascular diseases. The advent of EGFR monoclonal antibodies cetuximab and panitumumab has greatly broadened the efficacy of metastatic colorectal tumors, and people have realized the use of immunohistochemistry to detect EGFR.

[0003] There is no correlation between positive protein expression and the efficacy of EGFR monoclonal antibody treatment, so efforts have been made to study possible efficacy prediction markers. Gene mutations downstream of the EGFR signal transduction system, such as K-ras, BRAF, and PIK3CA, and inactivation of the tumor suppressor gene PTEN have become research hotspots. The existing test kit is placed with a pad, and a reagent slot for placing reagent tubes is provided on the pad. However, since the reagent slot is provided on the pad, only reagent tubes that are compatible with the reagent slot can be placed, and the specifications of the reagent tubes are different. Therefore, the test kit cannot fix reagent tubes of different specifications and sizes.

[0004] For example, a reagent device for detecting hotspot mutations of the EGFR gene disclosed in a Chinese patent document (Announcement No.: CN214422638U) further clamps the reagent tube by setting two clamping plates to ensure the stability of the reagent tube in the reagent kit. Due to the presence of elastic parts, the two clamping plates can automatically adapt to the size of the reagent tube and automatically adjust the distance between the two clamping plates.

[0005] However, when the specification of one of the reagent tubes is larger than that of the other two reagent tubes, the clamping plate cannot fix the other two reagent tubes. Moreover, when the reagent tubes are fixed by rotating the fixing screw to drive the movable clamping half ring and the fixed clamping half ring, since only the movable clamping half ring moves unilaterally, it is easy to cause the lower end of the reagent tube and the clamping part of the clamping plate to tilt, causing conflict between the two. This is not only cumbersome to operate, but also easy to cause the problem of reduced fixing effect of the reagent tube. Utility Model Content

[0006] In view of the defects in the prior art, the utility model provides a reagent device for detecting EGFR gene hot spot mutations, which can stably place and fix reagent tubes of different sizes, and is easy to operate during storage and retrieval, thereby improving the practical effect.

[0007] The utility model proposes a reagent device for detecting EGFR gene hotspot mutations, comprising a reagent storage shell, wherein the inner bottom wall of the reagent storage shell is provided with arc-shaped positioning grooves distributed in a linear array, the inner walls on both sides of the reagent storage shell are fixedly connected with fixed blocks distributed in a linear array, the upper surface of the fixed block is provided with a through hole, the upper surface of the fixed block is fixedly connected with an annular slide rail, the outer surface of the annular slide rail is slidably sleeved with a rotating ring, and the outer surface of the rotating ring is provided with anti-slip grooves distributed in an annular array;

[0008] The upper surface of the rotating ring is provided with a convenient fixing device, and the convenient fixing device comprises an annular mounting plate, and the lower surface of the annular mounting plate is fixedly connected to the upper surface of the rotating ring.

[0009] Preferably, a placement hole is provided at the center of the upper surface of the annular mounting plate, sliding grooves distributed in an annular array are provided on the upper surface of the annular mounting plate, and symmetrically distributed limiting sliding grooves are provided on the upper surface of the fixing block.

[0010] Preferably, the inner wall of the limiting sliding groove is slidably connected to a limiting sliding block, and a return spring is fixedly connected to a side surface of the limiting sliding block.

[0011] Preferably, one end of the return spring is fixedly connected to an inner wall of one side of the limiting sliding groove, and a sliding rod is fixedly connected to the upper surface of the limiting sliding block.

[0012] Preferably, the outer surface of the sliding rod is slidably inserted into the inner wall of the sliding groove, and one end of the sliding rod is fixedly connected to a moving block.

[0013] Preferably, one end of the moving block is fixedly connected to an arc-shaped clamping block, and the upper surface of the reagent storage shell is hinged with a protective cover through a hinge.

[0014] Preferably, a handle is fixedly connected to the upper surface of the protective cover.

[0015] The beneficial effects of the utility model are embodied in:

[0016] By setting up a convenient fixing device, reagent tubes of different sizes can be stably placed and fixed, and the operation is convenient during storage and retrieval, thereby improving the practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the specific implementation of the utility model or the technical solution in the prior art, the following is a brief introduction to the drawings required for the specific implementation or the prior art description. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, each element or part is not necessarily drawn according to the actual scale.

[0018] Figure 1 A front view of a reagent device for detecting EGFR gene hotspot mutations provided by the utility model;

[0019] Figure 2 for Figure 1 A three-dimensional diagram of the reagent storage shell structure of a reagent device for detecting EGFR gene hotspot mutations is shown;

[0020] Figure 3 for Figure 1 A three-dimensional diagram of the structure of a ring-shaped mounting plate of a reagent device for detecting EGFR gene hotspot mutations is shown;

[0021] Figure 4 for Figure 1 A three-dimensional diagram of the fixed block structure of a reagent device for detecting EGFR gene hotspot mutations is shown.

[0022] In the accompanying drawings, 1. reagent storage shell, 2. arc-shaped positioning groove, 3. fixed block, 4. through hole, 5. annular slide rail, 6. rotating ring, 7. anti-slip groove, 8. annular mounting plate, 81. placement hole, 82. sliding groove, 83. limiting slide groove, 84. limiting slider, 85. reset spring, 86. slide rod, 87. moving block, 88. arc-shaped clamping block, 89. protective cover, 810. handle. DETAILED DESCRIPTION

[0023] The following embodiments of the technical solution of the utility model are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the utility model, and are therefore only used as examples, and cannot be used to limit the protection scope of the utility model.

[0024] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by technicians in the field to which the utility model belongs.

[0025] Reference Figure 1-4 A reagent device for detecting hotspot mutations of EGFR gene, comprising a reagent storage shell 1, the inner bottom wall of the reagent storage shell 1 is provided with arc-shaped positioning grooves 2 distributed in a linear array, the arc-shaped positioning grooves 2 are used to support and position the lower end of the reagent tube, and the inner wall of the arc-shaped positioning groove 2 can protect the lower end of the reagent tube by arranging a rubber pad, the inner walls of the two sides of the reagent storage shell 1 are fixedly connected with fixed blocks 3 distributed in a linear array, the upper surface of the fixed block 3 is provided with a through hole 4, the upper surface of the fixed block 3 is fixedly connected with an annular slide rail 5, the outer surface of the annular slide rail 5 is slidably sleeved with a rotating ring 6, the outer surface of the rotating ring 6 is provided with anti-skid grooves 7 distributed in an annular array, the anti-skid grooves 7 are used to increase the friction between the finger and the rotating ring 6, so as to facilitate rotation;

[0026] The upper surface of the rotating ring 6 is provided with a convenient fixing device, and the convenient fixing device includes an annular mounting plate 8 , and the lower surface of the annular mounting plate 8 is fixedly connected to the upper surface of the rotating ring 6 .

[0027] A placement hole 81 is provided at the center of the upper surface of the annular mounting plate 8, and sliding grooves 82 distributed in a ring array are provided on the upper surface of the annular mounting plate 8. A symmetrically distributed limiting grooves 83 are provided on the upper surface of the fixed block 3. The inner wall of the limiting groove 83 is slidably connected to a limiting slider 84. A return spring 85 is fixedly connected to the surface of one side of the limiting slider 84, and one end of the return spring 85 is fixedly connected to the inner wall of one side of the limiting groove 83. A slide rod 86 is fixedly connected to the upper surface of the limiting slider 84. The elastic force of the return spring 85 drives the slide rod 86 to reset through the limiting slider 84, and the rotation of the annular mounting plate 8 drives the slide rod 86 to move through the cooperation of the sliding groove 82.

[0028] The outer surface of the slide bar 86 is slidably plugged into the inner wall of the slide groove 82, one end of the slide bar 86 is fixedly connected to a moving block 87, one end of the moving block 87 is fixedly connected to an arc-shaped clamping block 88, the arc-shaped clamping block 88 serves to clamp and fix the outer surface of the reagent tube, the upper surface of the reagent storage shell 1 is hinged with a protective cover body 89 through a hinge, the protective cover body 89 serves to protect the reagent tube inside the reagent storage shell 1, the upper surface of the protective cover body 89 is fixedly connected to a handle 810, the handle 810 serves to facilitate hand carrying.

[0029] By setting up a convenient fixing device, reagent tubes of different sizes can be stably placed and fixed, and the operation is convenient during storage and retrieval, thereby improving the practicality.

[0030] Working principle: Step 1, when the reagent tube needs to be placed and fixed, first hold the outer side of the rotating ring 6 and rotate it counterclockwise. The rotation of the rotating ring 6 is positioned and rotated through the annular slide rail 5, and drives the annular mounting plate 8 to rotate. The rotation of the annular mounting plate 8 squeezes the slide bar 86 through the cooperation of the sliding groove 82. The squeezed slide bar 86 drives the reset spring 85 to contract through the limit slider 84, and drives the two arc-shaped clamping blocks 88 to move back to each other through the moving block 87;

[0031] Step 2: at this time, the reagent tube to be placed is placed from the placement hole 81, and after the lower end of the reagent tube passes through the through hole 4, it contacts the arc-shaped positioning groove 2 at the bottom, and the bottom end of the reagent tube is supported and preliminarily positioned by the arc-shaped positioning groove 2, and then the rotating ring 6 is loosened. At this time, the elastic force of the reset spring 85 drives the limit slider 84 to reset and move through the guidance of the limit slide groove 83, and the movement of the limit slider 84 drives the two arc-shaped clamping blocks 88 to move relative to each other through the slide rod 86 and the moving block 87. The movement of the slide rod 86 drives the annular mounting plate 8 to reset and rotate through the slide groove 82. At the same time, the relative movement of the two arc-shaped clamping blocks 88 centrally positions the reagent tube and clamps it;

[0032] Step three, when the reagent tube needs to be taken out, hold the outer side of the rotating ring 6 again and rotate it counterclockwise so that the annular mounting plate 8 squeezes the sliding rod 86 through the sliding groove 82, so that the two arc-shaped clamping blocks 88 move away from each other and disengage from the clamping of the reagent tube, and then the reagent tube can be taken out.

[0033] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. These modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the utility model, and they should all be included in the scope of the claims and specification of the utility model.

Claims

1. A reagent device for detecting EGFR gene hotspot mutations, comprising a reagent storage housing (1), characterized in that: The inner bottom wall of the reagent storage shell (1) is provided with arc-shaped positioning grooves (2) distributed in a linear array, the inner walls on both sides of the reagent storage shell (1) are fixedly connected with fixed blocks (3) distributed in a linear array, the upper surface of the fixed block (3) is provided with a through hole (4), the upper surface of the fixed block (3) is fixedly connected with an annular slide rail (5), the outer surface of the annular slide rail (5) is slidably sleeved with a rotating ring (6), and the outer surface of the rotating ring (6) is provided with anti-slip grooves (7) distributed in an annular array; The upper surface of the rotating ring (6) is provided with a convenient fixing device, and the convenient fixing device comprises an annular mounting plate (8), and the lower surface of the annular mounting plate (8) is fixedly connected to the upper surface of the rotating ring (6).

2. A reagent device for detecting EGFR gene hotspot mutations according to claim 1, characterized in that: A placement hole (81) is provided at the center of the upper surface of the annular mounting plate (8), sliding grooves (82) distributed in an annular array are provided on the upper surface of the annular mounting plate (8), and symmetrically distributed limiting sliding grooves (83) are provided on the upper surface of the fixed block (3).

3. A reagent device for detecting EGFR gene hotspot mutations according to claim 2, characterized in that: The inner wall of the limiting sliding groove (83) is slidably connected to a limiting sliding block (84), and a return spring (85) is fixedly connected to a side surface of the limiting sliding block (84).

4. A reagent device for detecting EGFR gene hotspot mutations according to claim 3, characterized in that: One end of the return spring (85) is fixedly connected to an inner wall of one side of the limiting sliding groove (83), and a sliding rod (86) is fixedly connected to the upper surface of the limiting sliding block (84).

5. A reagent device for detecting EGFR gene hotspot mutations according to claim 4, characterized in that: The outer surface of the sliding rod (86) is slidably plugged into the inner wall of the sliding groove (82), and one end of the sliding rod (86) is fixedly connected to a moving block (87).

6. A reagent device for detecting EGFR gene hotspot mutations according to claim 5, characterized in that: One end of the moving block (87) is fixedly connected to an arc-shaped clamping block (88), and the upper surface of the reagent storage shell (1) is hingedly connected to a protective cover (89) via a hinge.

7. A reagent device for detecting EGFR gene hotspot mutations according to claim 6, characterized in that: A handle (810) is fixedly connected to the upper surface of the protective cover (89).

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