Carrier, temperature control device and PCR detection system

Abstract

The utility model relates to a carrier, a temperature control device and a PCR (Polymerase Chain Reaction) detection system. The carrier comprises a flexible bag, the bag comprises a first film and a second film, a reaction cavity is defined by the second film and used for containing reaction liquid, a temperature control cavity used for circulating a medium is formed between the first film and the second film, and the medium is used for heating and cooling the reaction liquid in the reaction cavity. The temperature control device comprises a heater and a cooler, the heater is used for providing a thermal medium with a preset temperature for the carrier, and the cooler is used for providing a cold medium with a preset temperature for the carrier. The PCR detection system comprises a fluorescence detection mechanism and a temperature control device, wherein the fluorescence detection mechanism is used for detecting an object to be detected in the reaction liquid. The carrier is low in cost, and the reaction liquid is high in heating and cooling speed.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro detection, in particular to a carrier, a temperature control device and a PCR detection system. Background Art

[0002] PCR (polymerase chain reaction) refers to a molecular biology experimental method for in vitro enzymatic synthesis of specific DNA fragments. PCR amplification consists of repeated thermal cycles of three steps: high-temperature denaturation, low-temperature annealing, and appropriate temperature extension.

[0003] In the prior art, the reaction solution needs to be placed in a carrier for PCR amplification. For the temperature control of the reaction solution in the carrier, one method is water bath temperature control, that is, placing the carrier in water or oil to heat or cool it. Another method is electronic component heating, such as single-sided heating or placing the carrier in a groove and heating around the carrier.

[0004] The defects of both temperature control methods are:

[0005] 1. The tightness of the contact between the carrier and the heater will affect the temperature control efficiency, and it is difficult for the carrier and the heater to be in full contact;

[0006] 2. The contact part between the carrier and the heater often uses a metal thermal conductive film, which is opaque and limits the direction of the fluorescence incident on the object to be tested;

[0007] 3. The main way to heat electronic components is that the cooler is often integrated with the heater. When the cooler cools the carrier, the heater needs to be cooled first, which causes power waste and reduces detection efficiency;

[0008] 4. The uneven thickness of the heater itself will affect the temperature control effect (for example, the thickness error of FR-4 is about ±0.1mm);

[0009] 5. The carrier needs to be made of a metal thermally conductive film. At the same time, in order to have good contact with the heater, a high processing accuracy is required, resulting in a relatively high cost of the carrier;

[0010] 6. The carrier is thick, which affects the heat transfer efficiency;

[0011] 7. The heating method in which the electronic components and the carrier are integrated requires the preparation of a new mold. Utility Model Content

[0012] One purpose of the utility model is to provide a carrier to at least solve one of the above technical problems.

[0013] To achieve the above-mentioned purpose, the first aspect of the utility model provides a carrier, which includes a flexible pouch, and the pouch includes a first membrane and a second membrane, the second membrane enclosing a reaction chamber, the reaction chamber is used to accommodate a reaction liquid, and a temperature control chamber for circulating a medium is formed between the first membrane and the second membrane, and the medium is used to heat and cool the reaction liquid in the reaction chamber.

[0014] Optionally, the carrier further includes a temperature measuring cavity, and the temperature measuring cavity is used to contain the fluorescent temperature-sensitive material.

[0015] Optionally, the temperature measuring chamber is arranged at one end of the reaction chamber.

[0016] Optionally, the number of the reaction chambers is one or more, and the multiple reaction chambers are independent of each other.

[0017] Optionally, the carrier is transparent.

[0018] Optionally, the pouch is made of plastic.

[0019] Optionally, the pouch is transparent.

[0020] Optionally, the thickness of the second film is 10 μm-50 μm, and / or

[0021] The thickness of the first film is 0.15 mm to 5 mm, and / or

[0022] The thickness of the reaction chamber is 0.1 mm-10 mm.

[0023] Optionally, the reaction chamber is a flat structure.

[0024] Optionally, the flat structure means that a dimension of the reaction chamber perpendicular to its thickness direction is larger than a dimension of the reaction chamber in the thickness direction.

[0025] Optionally, the ratio of the dimension of the reaction chamber perpendicular to its thickness direction to its thickness direction is greater than 5:1.

[0026] Optionally, the ratio of the dimensions is 25:1 to 500:1.

[0027] Optionally, there are multiple temperature control chambers.

[0028] Optionally, there are two temperature control chambers, and the two temperature control chambers are respectively arranged on two opposite sides of the reaction chamber.

[0029] Optionally, the two temperature control chambers are independent of each other, and the medium in the two temperature control chambers flows in opposite or same directions.

[0030] Optionally, the first membrane is provided with an inlet and an outlet, and the medium enters the temperature control chamber from the inlet and flows out of the temperature control chamber from the outlet.

[0031] Optionally, the outlet is located at a first end of the temperature control chamber, and the inlet is located at a second end of the temperature control chamber, and the first end is opposite to the second end.

[0032] Optionally, the second membrane includes a first inner membrane sheet and a second inner membrane sheet, the first inner membrane sheet and the second inner membrane sheet are arranged opposite to each other and connected to each other to form the reaction chamber; and / or

[0033] The first membrane includes a first outer membrane sheet and a second outer membrane sheet, the first outer membrane sheet and the second outer membrane sheet are located outside the second membrane and respectively located on opposite sides of the second membrane, the first outer membrane sheet is connected to the second membrane to form one temperature control cavity; the second outer membrane sheet is connected to the second membrane to form another temperature control cavity.

[0034] Optionally, there is one temperature control chamber, and the reaction chamber is surrounded by the temperature control chamber.

[0035] Another object of the present invention is to provide a temperature control device to at least solve one of the above technical problems.

[0036] To achieve this purpose, the second aspect of the utility model adopts the following technical solutions:

[0037] A temperature control device is used in conjunction with the carrier, the temperature control device comprises a heater and a cooler, the heater is used to provide a hot medium of a preset temperature to the carrier, and the cooler is used to provide a cold medium of a preset temperature to the carrier.

[0038] Optionally, the heater heats the medium by electromagnetic heating.

[0039] Optionally, when the temperature control device is used in conjunction with the carrier, the temperature control device also includes a circulation loop, one end of which can be connected to the inlet of the temperature control chamber, and the other end can be connected to the outlet of the temperature control chamber, the heater is used to heat the medium in the circulation loop, and the cooler can provide cold medium for the circulation loop.

[0040] Optionally, the circulation loop includes a liquid return pipeline, the liquid return pipeline includes a main liquid return pipe, a first liquid return branch pipe and a second liquid return branch pipe, the first liquid return branch pipe and the second liquid return branch pipe are selectively connected to the main liquid return pipe;

[0041] The first liquid return branch pipe is connected to the liquid outlet of the cooler, the second liquid return branch pipe is connected to the liquid return port of the cooler, and the heater is used to heat the medium downstream of the liquid outlet of the cooler; and / or

[0042] The circulation loop comprises a liquid inlet pipeline, one end of which is connected to the liquid outlet of the cooler, and the other end of which can be connected to the inlet of the temperature control chamber.

[0043] Optionally, the temperature control device further includes a temperature detection unit, and the temperature detection unit is used to detect the temperature of the medium.

[0044] Optionally, the temperature detection unit includes a first temperature detection unit and a second temperature detection unit, the first temperature detection unit is used to detect the medium temperature at one end of the circulation loop, and the second temperature detection unit is used to detect the medium temperature at the other end of the circulation loop.

[0045] Another object of the present invention is to provide a PCR detection system to at least solve one of the above technical problems.

[0046] To achieve this purpose, the third aspect of the present invention adopts the following technical solutions:

[0047] A PCR detection system comprises a fluorescence detection mechanism and a temperature control device, wherein the fluorescence detection mechanism is used to detect an object to be detected in a reaction solution.

[0048] As can be seen from the above, the technical solution provided by the utility model is that the medium used to cool and heat the carrier flows directly in the carrier, directly heating and cooling the carrier, and the medium and the carrier can be in full contact without any gaps, which completely avoids the situation where the contact between the carrier and the heater or cooler is not tight enough, greatly improving the temperature control efficiency and detection efficiency. At the same time, the processing accuracy requirements for the carrier are low, which can reduce the cost of the carrier. A temperature control cavity for circulating the medium is formed between the second membrane and the first membrane, and the second membrane surrounds the reaction cavity. Therefore, there is only the second membrane between the medium and the reaction liquid, and heat exchange can be quickly achieved between the medium and the reaction liquid.

[0049] Since the medium is in direct contact with the carrier and the heater does not need to be in contact with the carrier, whether the thickness of the heater itself used to heat the medium is uniform will not affect the temperature control effect, thus solving the problem of different temperature control effects caused by the degree of contact between the carrier and the temperature control structure (heater).

[0050] When it is necessary to switch from the state of heating the carrier to the state of cooling the carrier, it is only necessary to introduce cold medium into the temperature control chamber, and the hot medium will naturally flow out of the temperature control chamber, thereby saving power and energy and improving detection efficiency.

[0051] The pouch is transparent, and the carrier does not need to use a metal thermal conductive film, so that the direction of the fluorescence incident on the object to be detected is no longer limited, which reduces the difficulty of equipment design and solves the problem of limited fluorescence incident direction. The carrier includes the pouch, and the pouch itself has a small thickness, so heat can be quickly transferred between the reaction liquid and the medium. The carrier has a flat structure, so the thickness of the reaction liquid is small, and the temperature of the reaction liquid can quickly reach a uniform level. The pouch includes a first film and a second film, and the first film and the second film can be processed using existing molds, and are easy to mass produce with low processing costs.

[0052] The temperature is detected by the fluorescence detection method of the fluorescent temperature-sensitive material in the temperature measurement cavity, and the detection result is highly accurate, thereby improving the detection accuracy of the object to be measured. At the same time, the fluorescent temperature-sensitive material is not set in the reaction liquid, but is placed separately in the temperature measurement cavity, thereby avoiding affecting the detection of the reaction liquid and improving the accuracy of the detection result.

[0053] The temperature control device can be used in conjunction with the above-mentioned carrier, and the temperature control device includes a heater and a cooler. The heater is used to provide a hot medium with a preset temperature to the carrier, and the cooler is used to provide a cold medium with a preset temperature to the carrier. The cooler and the heater can provide a preset temperature medium for the temperature control chamber according to the target temperature of the reaction liquid. The cooler and the heater are separately designed as independent modules and work separately. When there is a problem with the temperature control, it is easy to check the heater and the cooler, and it is easy to solve the problem.

[0054] The heater heats the medium by electromagnetic heating, which can rapidly heat the medium. BRIEF DESCRIPTION OF THE DRAWINGS

[0055] Figure 1 It is a schematic diagram of the structure of the first carrier provided by the embodiment of the utility model;

[0056] Figure 2 It is a schematic diagram of the structure of the second carrier provided in the embodiment of the utility model;

[0057] Figure 3 It is a schematic diagram of the structure of the third carrier provided by the embodiment of the utility model;

[0058] Figure 4 It is a schematic structural diagram of the fourth carrier provided by the embodiment of the utility model;

[0059] Figure 5 is a schematic diagram of another viewing angle of the first carrier provided by an embodiment of the utility model;

[0060] Figure 6 It is a three-dimensional diagram of the first carrier provided by the embodiment of the utility model;

[0061] Figure 7 is a perspective view of a first carrier provided by an embodiment of the utility model;

[0062] Figure 8 is a cross-sectional view of the first carrier provided by an embodiment of the utility model;

[0063] Fig. 9 It is a schematic diagram of the structure of the fifth carrier provided by the embodiment of the utility model;

[0064] Fig.10 is a three-dimensional diagram of a fifth carrier provided by an embodiment of the utility model;

[0065] Fig.11 is a perspective view of a fifth carrier provided by an embodiment of the utility model;

[0066] Fig.12 is a cross-sectional view of a fifth carrier provided by an embodiment of the utility model;

[0067] Fig.13 is a schematic structural diagram of a sixth carrier provided by an embodiment of the utility model;

[0068] Fig.14 This is a schematic structural diagram of the sixth carrier provided by an embodiment of the utility model from another perspective;

[0069] Fig.15 is a schematic structural diagram of the seventh carrier provided by an embodiment of the utility model;

[0070] Fig.16 It is a schematic structural diagram of an eighth carrier provided by an embodiment of the utility model;

[0071] Fig.17a It is a schematic structural diagram of the connection between the temperature control device and the carrier provided in the embodiment of the utility model;

[0072] Fig.17b It is an amplification curve diagram measured in the fluorescence detection of Ureaplasma urealyticum provided by the embodiment of the utility model;

[0073] Fig.18 This is a schematic diagram of the first carrier liquid addition process provided by the embodiment of the utility model;

[0074] Fig.19 It is a schematic diagram of the fifth carrier liquid adding process provided by an embodiment of the utility model.

[0075] In the figure:

[0076] 1. Capsule; 11. Reaction chamber; 12. Temperature control chamber; 121. Inlet; 122. Outlet; 13. Second membrane; 131. First inner membrane sheet; 132. Second inner membrane sheet; 14. First membrane; 143. First outer membrane sheet; 144. Second outer membrane sheet; 145. Inlet and outlet; 16. Temperature measurement chamber; 17. Liquid injection port; 18. Partition;

[0077] 2. Heater; 3. Cooler; 31. Liquid outlet; 32. Liquid return outlet;

[0078] 4. Circulation loop; 41. Liquid return pipeline; 411. Main liquid return pipe; 412. First liquid return branch pipe; 413. Second liquid return branch pipe; 42. Liquid inlet pipeline;

[0079] 5. Quick connector; 6. Pump;

[0080] 10. Carrier; 20. Temperature control device;

[0081] 100. Liquid injection gun. DETAILED DESCRIPTION

[0082] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the utility model, rather than to limit the utility model. It is also necessary to explain that, for the convenience of description, only the parts related to the utility model are shown in the accompanying drawings, rather than all.

[0083] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "upper", "lower", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.

[0084] In the present utility model, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through another feature between them. Moreover, the first feature being "above", "above" and "above" the second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0085] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0086] Embodiment 1

[0087] This embodiment provides a carrier 10 for use in PCR amplification and detection, but is not limited thereto and can also be used in other occasions that require accommodating samples.

[0088] like Figure 1 As shown, the carrier 10 provided in this embodiment includes a reaction chamber 11 and a temperature control chamber 12 which are independent of each other. The reaction chamber 11 is used to contain the reaction liquid, and the temperature control chamber 12 is used to circulate the medium, and the medium is used to heat and cool the reaction liquid in the reaction chamber 11.

[0089] The medium used to cool and heat the carrier 10 flows directly in the carrier 10, directly heating and cooling the carrier 10, and the medium and the carrier 10 can be in full contact without any gap, completely avoiding the situation where the carrier 10 is not in close contact with the heater or cooler, greatly improving the temperature control efficiency and detection efficiency. At the same time, the processing accuracy requirements for the carrier 10 are low, which can reduce the cost of the carrier 10.

[0090] Since the medium is in direct contact with the carrier 10, the heater 2 (such as Fig.17a ) does not need to contact with the carrier 10, so whether the thickness of the heater 2 itself used to heat the medium is uniform will not affect the temperature control effect, solving the problem of different temperature control effects caused by the degree of contact between the carrier 10 and the temperature control structure (heater).

[0091] When the state of the heating carrier 10 needs to be switched to the state of the cooling carrier 10, it is only necessary to introduce cold medium into the temperature control chamber 12, and the hot medium will naturally flow out of the temperature control chamber 12, thereby saving power and energy and improving detection efficiency.

[0092] It is understandable that the medium may be a liquid medium such as water or oil having a boiling point higher than water, or a gaseous medium such as steam or low-temperature water mist.

[0093] Optionally, there are multiple temperature control chambers 12. Figure 1 , Figure 2 , Figure 8 and Fig.12 As shown, when there are two temperature control chambers 12, the temperature control chambers 12 can be set on opposite sides of the reaction chamber 11. At this time, the reaction liquid can reach the target temperature more quickly, improving the detection efficiency; preferably, the two temperature control chambers 12 are independent of each other, that is, the two temperature control chambers 12 flow into and out of the medium independently. Exemplarily, the medium in the two temperature control chambers 12 flows in opposite directions, so that the temperature of the reaction liquid is faster and uniform. Of course, the medium flow directions of the two temperature control chambers 12 can also be the same.

[0094] Optionally, the reaction chamber 11 is a flat structure. It is understood that the flat structure may mean that the dimension of the reaction chamber 11 perpendicular to its thickness direction is larger than the dimension in the thickness direction, for example, the dimension of the reaction chamber 11 in the thickness direction is smaller than the dimension in the width direction or the length direction, and as an example, the ratio of the dimension of the reaction chamber 11 perpendicular to its thickness direction to the dimension in its thickness direction is greater than 5:1, such as the dimension ratio is 50:1 to 100:1.

[0095] For example, the reaction chamber 11 is a cuboid (it should be noted that the cuboid may refer to a roughly cuboid, not an absolute cuboid in the geometric sense), the ratio of the length (or width) to the thickness of the cuboid may be greater than 5:1, such as a size ratio of 25:1 to 500:1, and as an example, the size ratio is 90:1, such as the size of the reaction chamber 11 in the thickness direction may be 0.1-1.0 mm, and the width and length of the reaction chamber 11 are about 10 mm and 20 mm, respectively. For example, for example, the length, width and thickness of the reaction chamber 11 are 50 mm, 5 mm, and 0.1 mm, respectively, which can accommodate 25 μL of reaction liquid, and for another example, the length, width and thickness of the reaction chamber 11 are 25 mm, 5 mm, and 0.2 mm, respectively, which can accommodate 25 μL of reaction liquid, and for another example, the length, width and thickness of the reaction chamber 11 are 10 mm*8.3 mm*0.3 mm, respectively, which can accommodate about 25 μL of reaction liquid.

[0096] As an example, the reaction chamber 11 can also be a cylindrical structure with a diameter-to-thickness ratio greater than 5:1, such as a thickness of 0.3-1.0 mm and a diameter of 5-20 mm. Of course, the cross-section of the reaction chamber 11 can be a polygon or an ellipse, etc. Of course, the cross-section of the reaction chamber 11 can be a polygon or an ellipse, etc.

[0097] The flat reaction chamber 11 has at least two surfaces with larger areas (such as Figure 5 The two surfaces are opposite to each other. For the convenience of explanation, the surface is named as the large surface. Optionally, when the temperature control chamber 12 is provided on both sides of the reaction chamber 11, the temperature control chamber 12 is provided on one side of the two large surfaces of the reaction chamber 11 (such as Figure 1 shown).

[0098] The reaction liquid in the flat reaction chamber 11 is thin, and the center of the reaction liquid is very close to the surface of the liquid. The temperature of the reaction liquid can reach a uniform level in a very short time. The flat structure can make the heat conduction area between the reaction chamber 11 and the temperature control chamber 12 large and the heat conduction efficiency high, thereby greatly improving the reaction liquid temperature rise and fall speed and detection efficiency.

[0099] Exemplarily, the thickness of the reaction chamber 11 is 0.1 mm-10 mm. Since the thickness of the reaction chamber 11 is very small, in this embodiment, the temperature control chamber 12 (such as Figure 1 However, the thickness of the reaction chamber 11 is not limited thereto. When the thickness of the reaction chamber 11 is relatively large, a temperature control chamber 12 may be correspondingly provided on the outer side of the reaction chamber 11.

[0100] like Figure 1-Figure 5 , Figure 7-Figure 9 , Figure 11-Figure 12 As shown, optionally, the outer contour of the reaction chamber 11 projected onto the temperature control chamber 12 is within the outer contour of the temperature control chamber 12, so that the liquid at various locations in the reaction chamber 11 can be heated or cooled by the medium.

[0101] like Figure 2 and Figure 5 As shown, when there are multiple temperature control chambers 12, the temperature control chamber 12 has an inlet 121 and an outlet 122. The medium enters the temperature control chamber 12 from the inlet 121 and flows out of the temperature control chamber 12 from the outlet 122. In this way, the medium flowing into the inlet 121 is the medium after the temperature is adjusted by the heater 2 or the cooler 3, so that the reaction liquid can reach the target temperature as soon as possible.

[0102] Optionally, the outlet 122 is located at the first end of the temperature control chamber 12 , and the inlet 121 is located at the second end of the temperature control chamber 12 , and the first end is opposite to the second end, so that the medium cools or heats the reaction liquid at various locations in the temperature control chamber 12 .

[0103] Optionally, a carrier 10 may include a reaction chamber 11 (eg Figure 5 As shown) or multiple reaction chambers 11 (as Fig. 9 When the carrier 10 includes multiple reaction chambers 11, the multiple reaction chambers 11 are independent of each other. The multiple reaction chambers 11 can be filled with different reaction solutions to simultaneously detect multiple different samples and improve the detection efficiency. The multiple reaction chambers 11 can also be filled with repeated reaction solutions to achieve the purpose of verifying the reaction results. Fig. 9 As shown, a carrier 10 has four reaction chambers 11, or as shown in FIG. Fig.11 and Fig.12 As shown, one carrier 10 has two reaction chambers 11 , but it is understandable that the number of reaction chambers 11 is not limited thereto.

[0104] like Fig. 9 , Fig.11 and Fig.12As shown, optionally, when the carrier 10 includes multiple reaction chambers 11, a temperature control chamber 12 is set on the same side of the multiple reaction chambers 11, and another temperature control chamber 12 is set on the other side of the multiple reaction chambers 11, that is, the multiple reaction chambers 11 share two temperature control chambers 12, which can make the structure of the carrier 10 simple and easy to process, and at the same time, it is easy to control the temperature of the multiple reaction chambers 11. The multiple reaction chambers 11 are arranged at intervals along the direction perpendicular to the flow of the carrier 10 (such as Fig. 9 ), so that the temperature of each reaction chamber 11 is uniform. Of course, it is also possible to Fig.11 As shown, a plurality of reaction chambers 11 are arranged at intervals along the flow direction of the carrier 10 .

[0105] like Figure 5 and Fig. 9 As shown, in order to make the medium flow rate in various places of the temperature control chamber 12 consistent, the inlet 121 and the outlet 122 of the temperature control chamber 12 are both long strips, so that the medium flows into the temperature control chamber 12 evenly.

[0106] like Figure 1 As shown, illustratively, the carrier 10 may also include a flexible pouch 1, which may form a temperature control chamber 12 and a reaction chamber 11, and the pouch 1 is transparent. Optionally, the pouch 1 is made of plastic, such as polypropylene (PP for short), the pouch 1 may also be made of polycarbonate (PC for short in English), and the pouch 1 may also be made of polyethylene terephthalate (PET for short). In the present embodiment, the pouch 1 is preferably made of PP or PC. In the present embodiment, the reaction chamber 11 and the temperature control chamber 12 are arranged in such a way that the reaction liquid can be cooled down quickly, and the pouch 1 (carrier 10) does not need to be made of a metal thermal conductive film, so that the cost of the carrier 10 is lower. Optionally, the carrier 10 is transparent, that is, the entire carrier 10 is transparent. The carrier 10 (pouch 1) does not need to use a metal thermal conductive film, so that the direction of the fluorescence incident on the object to be detected is no longer limited, which reduces the difficulty of equipment design and solves the problem of limited fluorescence incident direction.

[0107] Specifically, the pouch 1 includes a first film 14 and a second film 13, a temperature control cavity 12 for circulating the medium is formed between the second film 13 and the first film 14, and the second film 13 surrounds the reaction cavity 11, so that only the second film 13 exists between the medium and the reaction liquid, and heat exchange can be quickly achieved between the medium and the reaction liquid. The first film 14 and the second film 13 can be processed using existing molds without the need to re-open the mold, and the pouch 1 is easy to mass produce and has low processing costs.

[0108] like Figure 5-Figure 12 As shown, an inlet 121 and an outlet 122 are opened on the first membrane 14 , and the medium enters the temperature control chamber 12 through the inlet 121 and flows out of the temperature control chamber 12 through the outlet 122 .

[0109] like Figure 8 or Fig.12 As shown, when the temperature control chamber 12 is provided on opposite sides of the reaction chamber 11, the second film 13 may include a first inner film sheet 131 and a second inner film sheet 132, which are arranged opposite to each other and connected to each other to form the reaction chamber 11. Specifically, the first inner film sheet 131 and the second inner film sheet 132 may be plastic films, and the first inner film sheet 131 and the second inner film sheet 132 may be connected by welding. Of course, the first inner film sheet 131 and the second inner film sheet 132 may also be integrally formed.

[0110] Exemplarily, the first membrane 14 may include a first outer membrane sheet 143 and a second outer membrane sheet 144, which are located on the outside of the second membrane 13 and on opposite sides of the second membrane 13, respectively. The first outer membrane sheet 143 is connected to the second membrane 13 to form a temperature control cavity 12; the second outer membrane sheet 144 is connected to the second membrane 13 to form another temperature control cavity 12.

[0111] Furthermore, the first outer film sheet 143 is located on the outside of the first inner film sheet 131, and the second outer film sheet 144 is located on the outside of the second outer film sheet 144. The first outer film sheet 143 is connected to the first inner film sheet 131 to form a temperature control cavity 12, and the second outer film sheet 144 is connected to the second inner film sheet 132 to form another temperature control cavity 12. Specifically, the first outer film sheet 143 and the second outer film sheet 144 can be plastic films, and the first outer film sheet 143 and the second outer film sheet 144 can be connected by welding. Of course, the first outer film sheet 143 and the second outer film sheet 144 can also be integrally formed. The first inner film sheet 131, the second inner film sheet 132, the first outer film sheet 143 and the second outer film sheet 144 are all transparent to form a transparent pouch 1.

[0112] In this embodiment, when the carrier 10 includes two temperature control chambers 12, the pouch 1 is essentially formed by connecting four layers of membranes (from one side to the other, the first outer membrane sheet 143, the first inner membrane sheet 131, the second inner membrane sheet 132 and the second outer membrane sheet 144) in sequence. The structure and preparation method of the pouch 1 are very simple, the cost is very low, and it is suitable for mass production.

[0113] like Figure 3 and Figure 4 As shown, the number of temperature control chambers 12 can be one, and the reaction chamber 11 is surrounded by one temperature control chamber 12. The reaction chamber 11 can be completely immersed in the medium of the temperature control chamber 12, which can further increase the heating and cooling speed. At this time, the second film 13 surrounds the reaction chamber 11, and the second film 13 is placed in the temperature control chamber 12 surrounded by the first film 14. Figure 4As shown, optionally, the first membrane 14 may also be provided with an inlet and outlet 145 for the second membrane 13 to enter and exit, so as to facilitate immersing the second membrane 13 in the medium of the reaction chamber 11. When the number of reaction chambers 11 is one, the second membrane 13 with one reaction chamber 11 is located in the temperature control chamber 12; when the number of reaction chambers 11 is multiple, the second membrane 13 with multiple reaction chambers 11 is located in the temperature control chamber 12.

[0114] Optionally, when there are one or more temperature control chambers 12 , the first membrane 14 and the second membrane 13 may be connected to fix the position of the second membrane 13 , thereby preventing the second membrane 13 from shifting under the flow of the medium.

[0115] For example, Fig. 9 As shown, when there are multiple reaction chambers 11 in the carrier 10, multiple partitions 18 can be formed between the first inner film 131 and the second inner film 132 to make two adjacent reaction chambers 11 independent of each other. The partitions 18 can be formed by welding.

[0116] Optionally, the thickness of the second film 13 can be 10μm-50μm. The thickness of the first film 14 can be 0.15mm-5mm. It is understandable that the thickness of the first film 14 and the second film 13 is not limited thereto, as long as they have sufficient strength to avoid the carrier 10 from breaking. Optionally, the heating and cooling speed of the reaction solution can be increased by reducing the thickness of the second film 13. When the thickness of the second film 13 is reduced to 10μm, the heating and cooling speed can be increased by reducing the thickness of the reaction solution. In the industry, it is generally believed that transparent polypropylene film and polycarbonate film are difficult to withstand high temperatures at this thickness, and they are easy to break due to insufficient strength, making it difficult for the carrier to carry a certain volume of liquid. However, the utility model inventor unexpectedly discovered that polypropylene film and polycarbonate film can carry a certain volume of PCR reaction solution at this thickness and temperature to complete the amplification reaction.

[0117] like Fig.12 As shown, the carrier 10 may further include a quick connector 5, and both the inlet 121 and the outlet 122 may be connected to the quick connector 5, so that the carrier 10 is conveniently connected to the temperature control device 20. Optionally, the quick connector 5 may be made of plastic and connected to the pouch 1 by welding. Optionally, the quick connector 5 is transparent, so that the entire carrier 10 is transparent, so that the incident direction of the fluorescence is not restricted.

[0118] like Fig.13As shown, optionally, the carrier 10 may further include a temperature measuring chamber 16, and the temperature measuring chamber 16 is used to hold a fluorescent temperature-sensitive material. It is understandable that the temperature control chamber 12 can heat and cool the temperature measuring chamber 16 so that the temperature in the temperature measuring chamber 16 is consistent with the temperature in the reaction chamber 11, and thus the temperature of the fluorescent temperature-sensitive material is consistent with the temperature of the reaction liquid. In this embodiment, the temperature is detected by a fluorescent detection method of the fluorescent temperature-sensitive material in the temperature measuring chamber 16. Since the temperature of the temperature measuring chamber 16 is consistent with the temperature of the reaction chamber 11, the temperature of the reaction liquid in the reaction chamber 11 can be obtained. The fluorescence detection temperature result is highly accurate, thereby improving the detection accuracy. At the same time, the fluorescent temperature-sensitive material is not disposed in the reaction liquid, but is placed separately in the temperature measuring chamber 16, thereby avoiding affecting the detection of the reaction liquid and improving the accuracy of the detection result.

[0119] like Figure 13-Figure 16 As shown, the temperature measuring chamber 16 is disposed at one end of the reaction chamber 11 . It should be noted that the reaction chamber 11 can be disposed at any end of the temperature measuring chamber 16 as long as it does not affect the detection of the reaction liquid. Furthermore, the temperature measuring chamber 16 can be formed in the second film 13 .

[0120] like Fig.15 As shown, when the carrier 10 includes a plurality of reaction chambers 11, a temperature measuring chamber 16 may be provided at one end of each reaction chamber 11; or Fig.16 As shown, a temperature measurement chamber 16 may also be provided at one end of the plurality of reaction chambers 11 to save detection costs and carrier 10 costs.

[0121] It has been verified through experiments that when the vector 10 provided in this embodiment is used for amplification, the maximum temperature difference of the reaction solution is within 0.5°C, that is, at the same time, the highest temperature and the lowest temperature of the reaction solution differ by less than 0.5°C (specific experimental data will be described in detail later).

[0122] The carrier 10 provided in this embodiment uses simple materials (basically only requires the first film 14 and the second film 13); the processing technology is a conventional process, such as welding and cutting of plastics; it is easy to produce and low in cost, such as both material cost and processing cost are very low.

[0123] like Fig.17a As shown, this embodiment also provides a temperature control device 20, which can be used in conjunction with the above-mentioned carrier 10. The temperature control device 20 includes a heater 2 and a cooler 3. The heater 2 is used to provide a hot medium at a preset temperature to the carrier 10, and the cooler 3 is used to provide a cold medium at a preset temperature to the carrier 10. The cooler 3 and the heater 2 can provide a preset temperature medium for the temperature control chamber 12 according to the target temperature of the reaction liquid. The cooler 3 and the heater 2 are separately designed as independent modules and work separately. When there is a problem with the temperature control, it is convenient to check the heater 2 and the cooler 3 and solve the problem.

[0124] When the carrier 10 includes an inlet 121 and an outlet 122, the temperature control device 20 also includes a circulation loop 4, one end of the circulation loop 4 can be connected to the inlet 121 of the temperature control chamber 12, and the other end can be connected to the outlet 122 of the temperature control chamber 12, the heater 2 is used to heat the medium in the circulation loop 4, and the cooler 3 can provide cold medium for the circulation loop 4. When it is necessary to provide hot medium for the temperature control chamber 12, the heater 2 works to heat the medium in the circulation loop 4. The heater 2 does not need to store hot medium, but only needs to heat the medium in the circulation loop 4. Therefore, the heater 2 needs to heat a small amount of medium, and the medium can quickly reach a preset temperature. When it is necessary to provide cold medium for the temperature control chamber 12, the heater 2 no longer works or works at a lower power, and the cooler 3 provides cold medium for the circulation loop 4, and the medium in the circulation loop 4 and the temperature control chamber 12 is replaced by the cold medium. The temperature of the cold medium provided by the cooler 3 can be consistent with or lower than the temperature required for the PCR reaction. The cooler 3 can provide medium for the circulation loop 4 as needed. For example, the cold medium in the cooler 3 can completely replace the medium in the circulation loop 4, so that the medium temperature in the circulation loop 4 is consistent with the temperature of the cold medium in the cooler 3, or the cold medium in the cooler 3 can replace part of the medium in the circulation loop 4, so that the medium temperature in the circulation loop 4 is higher than the temperature of the cold medium in the cooler 3.

[0125] Furthermore, a loop can be formed between the temperature control chamber 12, the circulation loop 4 and the cooler 3. When the temperature control chamber 12 needs to be cooled, the heat medium in the circulation loop 4 and the temperature control chamber 12 flows back to the cooler 3, and the heat medium is cooled.

[0126] like Fig.17a As shown, temperature control chambers 12 are provided on opposite sides of the reaction chamber 11, and the temperature control device 20 is provided with two circulation loops 4 (the single dotted line represents one circulation loop 4, and the double dotted line represents another circulation loop 4), one circulation loop 4 is connected to one temperature control chamber 12, the heater 2 can heat the medium in the two circulation loops 4, and the cooler 3 can provide cold medium for the two circulation loops 4.

[0127] Exemplarily, the circulation loop 4 includes a liquid inlet pipeline 42, one end of which is connected to the liquid outlet 31 of the cooler 3, and the other end can be connected to the inlet 121 of the temperature control chamber 12. Exemplarily, the heater 2 is used to heat the medium downstream of the liquid outlet 31 of the cooler 3. Furthermore, the heater 2 is located between the liquid inlet pipelines 42 of the two circulation loops 4 to provide heat medium to the two liquid inlet pipelines 42 respectively. Exemplarily, the liquid outlet 31 of the cooler 3, the heater 2 and the liquid inlet pipeline 42 are connected in sequence.

[0128] Optionally, the cold medium flowing out of the liquid outlet 31 enters the liquid inlet pipeline 42 through the heater 2. When it is necessary to provide a hot medium, the outlet 122 of the cooler 3 is closed, and the heater 2 heats the medium in the circulation loop 4. The medium circulates between the heater 2, the circulation loop 4 and the temperature control chamber 12, thereby increasing the medium heating rate in the circulation loop 4, reducing the power of the heater 2, and reducing energy consumption. When the temperature of the reaction solution is slightly higher than the target temperature, the heater 2 can work or stop working at low power, and the cooler 3 provides a preset amount of cold medium for the circulation loop 4 through the liquid outlet 31, so that the reaction solution quickly reaches the target temperature. When the carrier 10 needs to enter the low temperature annealing stage from the high temperature denaturation stage, etc., the reaction solution needs to be cooled, the heater 2 stops working, and the cooler 3 provides a cold medium for the liquid inlet pipeline 42 through its liquid outlet 31.

[0129] The circulation loop 4 may further include a liquid return pipeline 41 , which includes a main liquid return pipe 411 , a first liquid return branch pipe 412 , and a second liquid return branch pipe 413 . The first liquid return branch pipe 412 and the second liquid return branch pipe 413 are selectively connected to the main liquid return pipe 411 .

[0130] Exemplarily, the liquid return pipeline 41 can be a pipeline. When it is a pipeline, the liquid return pipeline 41, the first liquid return branch pipe 412 and the second liquid return branch pipe 413 can be connected by a three-way valve, and the three-way valve can control the opening and closing of the first liquid return branch pipe 412 and the second liquid return branch pipe 413. Of course, the main liquid return pipe 411 itself can be a three-way valve, one port of the three-way valve is connected to the outlet 122 of the temperature control chamber 12, the second port is connected to the first liquid return branch pipe 412, and the third port is connected to the second liquid return branch pipe 413. The three-way valve can control the opening and closing of the first liquid return branch pipe 412 and the second liquid return branch pipe 413.

[0131] The first liquid return pipe 412 is connected to the liquid outlet 31 of the cooler 3, so that when the reaction liquid is heated up and needs to be maintained in the high-temperature denaturation and suitable temperature extension stages, the cooler 3 does not provide cold medium, and the hot medium does not need to pass through the inside of the cooler 3. The heater 2 only needs to replenish the heat lost by the hot medium. It can be understood that the heat loss is the heat loss of the hot medium when the reaction liquid is heated up and the heat loss caused by heat dissipation during the circulation of the hot medium.

[0132] The second liquid return branch pipe 413 is connected to the liquid return port 32 of the cooler 3. When the reaction liquid needs to be cooled, the cold medium can return to the cooler 3, and then the cold zone device can adjust the temperature of the cold medium.

[0133] Optionally, the liquid outlet 31 of the cooler 3 may be provided with a one-way valve, which may allow the medium in the cooler 3 to flow out while preventing the medium in the circulation loop 4 from flowing back into the cooler 3 .

[0134] Optionally, the lengths of the pipelines in the two circulation loops 4 are the same, that is, the two liquid inlet pipelines 42 are the same length, and the two liquid return pipelines 41 are the same length, so as to ensure that the medium temperature in the two temperature control chambers 12 is consistent, and then ensure that the temperature of each point of the reaction liquid is consistent. For example, the length of the pipeline in the circulation loop 4 can be adjusted by adjusting the position of the heater 2 and the cooler 3.

[0135] Exemplarily, the heater 2 heats the medium by electromagnetic heating, and electromagnetic heating can rapidly heat the medium. The heater 2 may include a metal tube and a coil, the coil being arranged outside the metal tube, the metal tube being connected to the liquid inlet pipeline 42, thereby providing heat medium to the liquid inlet pipeline 42, and the metal tube being connected to the liquid outlet 31 of the cooler 3, so that the medium in the cooler 3 enters the liquid inlet pipeline 42 through the metal tube.

[0136] When heating the medium, an alternating current is passed into the coil, thereby generating eddy currents in the metal tube. The temperature of the metal tube increases under the action of the eddy currents, and the medium flows through the metal tube, and the metal tube transfers heat to the medium. Optionally, the metal tube can be part of the circulation loop 4.

[0137] Exemplarily, the cooler 3 may include a water tank and a refrigeration element, the water tank may store the medium, and the refrigeration element may cool the medium.

[0138] Optionally, the temperature control device 20 may further include a pump 6, which may be connected to the liquid outlet 31 of the cooler 3. The pump 6 may increase the flow rate of the medium in the circulation loop 4 and the temperature control chamber 12, further increase the temperature rise and fall rate of the reaction liquid, and improve the detection efficiency.

[0139] In the prior art, both the heater and the cooler require a water tank, in which the medium is stored. When the temperature needs to be changed, the medium in the water tank must be heated or cooled, which results in slow heating and cooling speeds and a large volume and mass of the entire machine. In this embodiment, the heater 2 only needs to heat the medium in the circulation loop 4, so the temperature of the medium can be quickly adjusted, and the volume and mass of the entire machine can be small.

[0140] Optionally, the temperature control device 20 further includes a temperature detection unit, which is used to detect the temperature of the medium. Exemplarily, the temperature detection unit includes a first temperature detection unit and a second temperature detection unit, wherein the first temperature detection unit is used to detect the temperature of the medium at one end of the circulation loop 4. The second temperature detection unit is used to detect the temperature of the medium at the other end of the circulation loop 4. One end of the circulation loop 4 is connected to the inlet 121 of the temperature control chamber 12, so the first temperature detection unit can accurately detect the temperature T1 of the medium entering the temperature control chamber 12, and the other end of the circulation loop 4 is connected to the outlet 122 of the temperature control chamber 12, so the second temperature detection unit can accurately detect the temperature T2 of the medium flowing out of the temperature control chamber 12.

[0141] Furthermore, the first temperature detection unit is disposed on the liquid inlet pipeline 42 , and the second temperature detection unit is disposed on the liquid return pipeline 41 .

[0142] After experimental verification, when the initial temperature of the reaction liquid is 0°C and the volume of the reaction liquid is 25μL, the reaction liquid with different thicknesses (0.3mm, 0.2mm, 0.1mm respectively), different water flow rates in the temperature control chamber 12 (water flow rates ≥2ml / s, ≥4ml / s, ≥0.8ml / s, ≥0.6ml / s, ≥0.7ml / s respectively), and different thicknesses of the second membrane 13 (10μm and 20μm) were tested and verified. The test results show that after 1s, the temperature of the reaction liquid reaches the target temperature of 95°C, the heating rate of the reaction liquid is faster than the heating rate of 80℃ / s, and the temperature difference of the reaction liquid is less than 0.5℃. It can be understood that the above 1s is the time required for the reaction liquid to change temperature, not the time required for the medium to heat up.

[0143] In another specific experiment, the size of the reaction chamber 11 is 0.5mm*20mm*5mm, and the volume is 50μL (the reaction liquid can be increased to 100μL). The two temperature control chambers 12 on both sides of the reaction chamber 11 are respectively connected to water flow for heat conduction. The size of the temperature control chamber 12 is 1mm*20mm*5mm=100μL.

[0144] like Fig.17a As shown, the experiment is carried out by taking heating the reaction liquid as an example: the pump 6 draws water from the cooler 3, so that the water fills the circulation loop 4 and the temperature control chamber 12, the liquid outlet 31 of the cooler 3 is closed, and the pump 6 continues to work so that the water flow rate is F, and enters the heater 2 with a power of P. The water temperature after heating is T1, and the water flows into the carrier 10 and exchanges heat with the reaction liquid. T0 is the initial temperature of the reaction liquid, and T2 is the water temperature flowing out of the carrier 10 after the water flows and the reaction liquid exchanges heat. Within 1s, T1=T2=T0 can be achieved, and the heating and cooling speed of the reaction liquid is faster than 100℃ / s, and the maximum and minimum values ​​of the reaction liquid temperature differ by less than 0.5℃. It can be understood that the above 1s is the time required for the reaction liquid to change temperature, not the time required for the medium to heat up.

[0145] In a specific experiment, in the fluorescence detection of Ureaplasma urealyticum, the carrier 10 and the temperature control device 20 provided in this embodiment can complete PCR amplification within 10 minutes. 6 Amplification curves at different template concentrations (eg Fig.17b The Ct value was 27.48, and the curve was a typical "S-type", which met the requirements of qPCR amplification.

[0146] Embodiment 2

[0147] like Fig.18As shown, this embodiment 2 provides a sample adding method, which is used to add reaction liquid to the carrier 10 of embodiment 1, and the sample adding method includes:

[0148] S1, forming a reaction chamber 11 having a liquid injection port 17;

[0149] S2, injecting the reaction liquid into the reaction chamber 11; illustratively, the injection gun 100 or the like penetrates into the injection port 17, thereby injecting the reaction liquid into the reaction chamber 11;

[0150] S3, sealing the liquid injection port 17;

[0151] S4, forming a temperature control chamber 12 outside the reaction chamber 11.

[0152] In this embodiment, the reaction liquid is injected during the formation of the carrier 10, so that the rapid temperature rise and fall of the reaction liquid is achieved.

[0153] Optionally, step S1 includes: cutting a film sheet of a preset size from a film to form the second film 13; for example, a section of the film may be cut from a roll of a longer film to form the second film 13;

[0154] Optionally, step S1 may further include: connecting two layers of second films 13 disposed opposite to each other, and forming a reaction chamber 11 having a liquid injection port 17 between the two layers of second films 13. For example, the two layers of second films 13 are welded, and a portion of the positions are reserved for non-welding, so as to form the liquid injection port 17 at a preset position. It is understandable that at this time, the two layers of second films 13 are respectively the first inner film sheet 131 and the second inner film sheet 132 in the embodiment.

[0155] When the carrier 10 includes two temperature-controlled chambers 12 , step S4 includes: connecting the first film 14 to the outer side of the second film 13 to form the temperature-controlled chamber 12 between the second film 13 and the first film 14 .

[0156] like Fig.18 As shown, before step S4, the following steps may also be included:

[0157] S5, cutting a film sheet of a preset size from a film to form a first film 14. For example, the first film 14 can be formed by cutting a section of film from a longer roll of film.

[0158] In this embodiment, when the carrier 10 includes two temperature control chambers 12, the pouch 1 is essentially formed by connecting four layers of membranes (from one side to the other, the first outer membrane sheet 143, the first inner membrane sheet 131, the second inner membrane sheet 132 and the second outer membrane sheet 144) in sequence. The structure and preparation method of the pouch 1 are very simple and the cost is very low.

[0159] After step S5, the following steps may also be included:

[0160] S6, accurate quick connector 5;

[0161] S7 . Connect the quick connector 5 at the inlet 121 and the outlet 122 of the first membrane 14 .

[0162] Step S4 may refer to connecting the first membrane 14 connected with the quick connector 5 to the outside of the second membrane 13 .

[0163] like Fig.19 As shown, when there are multiple reaction chambers 11, step S1 may refer to forming multiple reaction chambers 11, each reaction chamber 11 correspondingly formed with a liquid injection port 17; illustratively, two layers of second films 13 arranged opposite to each other may be connected, and multiple partitions 18 may be arranged on the two layers of second films 13 at intervals, and the partitions 18 may be formed by welding the two layers of second films 13 to form multiple reaction chambers 11. One end of the two layers of second films 13 is not connected to form the liquid injection port 17 of each reaction chamber 11. It can be understood that at this time, the two layers of second films 13 are respectively the first inner film sheet 131 and the second inner film sheet 132 in the embodiment.

[0164] Optionally, step S2 may include: injecting the reaction solution into the reaction chamber 11 respectively. Fig.19 As shown, a plurality of injection guns 100 may inject liquid simultaneously, or one injection gun 100 may inject liquid into a plurality of reaction chambers 11 respectively.

[0165] Exemplarily, step S3 may refer to: sealing all the liquid injection ports 17 , such as sealing all the liquid injection ports 17 by welding two layers of the second film 13 .

[0166] Exemplarily, step S4 may refer to: connecting the first membrane 14 connected with the quick connector 5 to the outside of the second membrane 13 .

[0167] In other optional embodiments, the second film 13 of a preset size can be cut while the liquid injection port 17 is being welded, thereby improving the liquid injection efficiency and the detection efficiency.

[0168] Embodiment 3

[0169] The third embodiment provides a temperature control method. The temperature control device 20 of the first embodiment can control the temperature of the reaction liquid in the carrier 10 by using the temperature control method. The temperature control method includes:

[0170] The temperature control device 20 introduces a cold medium or a hot medium into the temperature control chamber 12 to increase, decrease or keep the temperature of the reaction liquid.

[0171] By adjusting the temperature of the reaction liquid by directly contacting the medium with the carrier 10, it is effectively solved that the tightness of the contact between the carrier 10 and the heater 2 affects the temperature control efficiency, and the problem that the carrier 10 and the heater 2 are difficult to achieve complete contact, the problem that the direction of the fluorescence incident on the object to be tested is limited because of the need to use a metal thermal conductive film in the contact part between the carrier 10 and the heater 2, and the problem that the uneven thickness of the heater 2 itself affects the temperature control effect.

[0172] Exemplarily, the temperature control device 20 includes a circulation loop 4;

[0173] When the reaction liquid is heated up, the heater 2 heats the medium in the circulation loop 4, and the medium circulates between the circulation loop 4 and the temperature control chamber 12; the cooler 3 does not provide cold medium, and the hot medium does not need to pass through the inside of the cooler 3. The heater 2 only needs to replenish the heat lost by the hot medium. It can be understood that the heat loss is the heat loss of the hot medium when the reaction liquid is heated up and the heat loss caused by heat dissipation during the circulation of the hot medium.

[0174] When the reaction liquid is cooled, the medium circulates between the cooler 3, the circulation loop 4 and the temperature control chamber 12. When the reaction liquid needs to be cooled, the cold medium can return to the cooler 3, and then the cold zone device can adjust the temperature of the cold medium.

[0175] Embodiment 4

[0176] This embodiment also provides a PCR detection system, which includes a fluorescence detection mechanism and the temperature control device 20 in the first embodiment, wherein the fluorescence detection mechanism is used to detect the object to be detected in the reaction solution. The PCR detection system has high detection efficiency and high detection result accuracy.

[0177] The PCR detection system can also use the sample adding method in the second embodiment to add the reaction solution to the carrier 10. Optionally, the PCR detection system can also use the temperature control method in the third embodiment to control the temperature of the reaction solution in the carrier 10.

[0178] The PCR detection system may further include a temperature detection mechanism, which may emit excitation light to the fluorescent temperature-sensitive material and detect the fluorescence intensity at the fluorescent temperature-sensitive material, thereby obtaining the temperature of the fluorescent temperature-sensitive material.

[0179] The PCR detection system may further include a control mechanism, which may be electrically connected to the temperature detection mechanism, the fluorescence detection mechanism and the temperature control device 20. The control mechanism may be a centralized or distributed controller, for example, the controller may be a single microcontroller or may be composed of multiple distributed microcontrollers, in which a control program may be run to control the above components to realize their functions.

[0180] Although the utility model has been described in detail above by means of general description, specific implementation methods and tests, it is obvious to those skilled in the art that some modifications or improvements can be made to the utility model. Therefore, these modifications or improvements made without departing from the spirit of the utility model are within the scope of protection claimed by the utility model.

Claims

1. A carrier, It is characterized in that The carrier comprises a flexible bag (1), the bag (1) comprising a first film (14) and a second film (13), the second film (13) enclosing a reaction chamber (11), the reaction chamber (11) being used to contain a reaction liquid, and a temperature control chamber (12) for circulating a medium is formed between the first film (14) and the second film (13), the medium being used to heat and cool the reaction liquid in the reaction chamber (11).

2. The vector according to claim 1, It is characterized in that The carrier further comprises a temperature measuring cavity (16), wherein the temperature measuring cavity (16) is used to contain the fluorescent temperature-sensitive material.

3. The vector according to claim 2, It is characterized in that The temperature measuring chamber (16) is arranged at one end of the reaction chamber (11).

4. The vector according to claim 1, It is characterized in that The number of the reaction chambers (11) is one or more, and the multiple reaction chambers (11) are independent of each other.

5. The vector according to claim 1, It is characterized in that The carrier is transparent.

6. The vector according to claim 1, It is characterized in that The bag (1) is made of plastic.

7. The vector according to claim 1, It is characterized in that The capsule (1) is transparent.

8. The vector according to claim 1, It is characterized in that The thickness of the second film (13) is 10 μm-50 μm, and / or The thickness of the first film (14) is 0.15 mm to 5 mm, and / or The thickness of the reaction chamber (11) is 0.1 mm-10 mm.

9. The vector according to claim 1, It is characterized in that The reaction chamber (11) is a flat structure.

10. The vector according to claim 9, It is characterized in that The flat structure means that the dimension of the reaction chamber (11) perpendicular to its thickness direction is larger than its thickness direction.

11. The vector according to claim 10, It is characterized in that The ratio of the dimension of the reaction chamber (11) perpendicular to its thickness direction to its thickness direction is greater than 5:

1.

12. The vector according to claim 11, It is characterized in that The ratio of the dimensions is 25:1 to 500:

1.

13. The vector according to claim 1, It is characterized in that The temperature control chambers (12) are multiple in number.

14. The vector according to claim 13, It is characterized in that There are two temperature control chambers (12), and the two temperature control chambers (12) are respectively arranged on two opposite sides of the reaction chamber (11).

15. The vector according to claim 14, It is characterized in that The two temperature control chambers (12) are independent of each other, and the medium in the two temperature control chambers (12) flows in opposite or same directions.

16. The vector according to any one of claims 1 to 15, It is characterized in that The first membrane (14) is provided with an inlet (121) and an outlet (122); the medium enters the temperature control chamber (12) through the inlet (121) and flows out of the temperature control chamber (12) through the outlet (122).

17. The vector according to claim 16, It is characterized in that The outlet (122) is located at a first end of the temperature control chamber (12), and the inlet (121) is located at a second end of the temperature control chamber (12), wherein the first end is opposite to the second end.

18. The vector according to claim 13, It is characterized in that The second membrane (13) comprises a first inner membrane sheet (131) and a second inner membrane sheet (132), wherein the first inner membrane sheet (131) and the second inner membrane sheet (132) are arranged opposite to each other and are connected to each other to form the reaction chamber (11); and / or The first membrane (14) comprises a first outer membrane sheet (143) and a second outer membrane sheet (144); the first outer membrane sheet (143) and the second outer membrane sheet (144) are located outside the second membrane (13) and are respectively located on opposite sides of the second membrane (13); the first outer membrane sheet (143) and the second membrane (13) are connected to form one temperature control cavity (12); the second outer membrane sheet (144) and the second membrane (13) are connected to form another temperature control cavity (12).

19. The vector according to any one of claims 1 to 12, It is characterized in that The number of the temperature control chamber (12) is one, and the reaction chamber (11) is surrounded by the temperature control chamber (12).

20. A temperature control device, It is characterized in that Used in conjunction with the carrier described in any one of claims 1 to 19, the temperature control device includes a heater (2) and a cooler (3), the heater (2) is used to provide a hot medium at a preset temperature to the carrier, and the cooler (3) is used to provide a cold medium at a preset temperature to the carrier.

21. The temperature control device according to claim 20, It is characterized in that The heater (2) heats the medium by electromagnetic heating.

22. The temperature control device according to claim 20, It is characterized in that When the temperature control device is used in conjunction with the carrier described in any one of claims 1 to 18, the temperature control device also includes a circulation loop (4), one end of the circulation loop (4) can be connected to the inlet (121) of the temperature control chamber (12), and the other end can be connected to the outlet (122) of the temperature control chamber (12), the heater (2) is used to heat the medium in the circulation loop (4), and the cooler (3) can provide cold medium for the circulation loop (4).

23. The temperature control device according to claim 22, It is characterized in that The circulation loop (4) comprises a liquid return pipeline (41), wherein the liquid return pipeline (41) comprises a main liquid return pipe (411), a first liquid return branch pipe (412) and a second liquid return branch pipe (413), wherein the first liquid return branch pipe (412) and the second liquid return branch pipe (413) are selectively connected to the main liquid return pipe (411); The first liquid return branch pipe (412) is in communication with the liquid outlet (31) of the cooler (3), the second liquid return branch pipe (413) is in communication with the liquid return port (32) of the cooler (3), and the heater (2) is used to heat the medium downstream of the liquid outlet (31) of the cooler (3); and / or The circulation loop (4) comprises a liquid inlet pipeline (42), one end of which is in communication with the liquid outlet (31) of the cooler (3), and the other end of which can be in communication with the inlet (121) of the temperature control chamber (12).

24. The temperature control device according to claim 22, It is characterized in that The temperature control device further comprises a temperature detection unit, and the temperature detection unit is used to detect the temperature of the medium.

25. The temperature control device according to claim 24, It is characterized in that The temperature detection unit comprises a first temperature detection unit and a second temperature detection unit, the first temperature detection unit being used to detect the temperature of the medium at one end of the circulation loop (4), and the second temperature detection unit being used to detect the temperature of the medium at the other end of the circulation loop (4).

26. A PCR detection system, It is characterized in that It comprises a fluorescence detection mechanism and a temperature control device according to any one of claims 20 to 25, wherein the fluorescence detection mechanism is used to detect an object to be detected in a reaction solution.

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