Calibrating device for photometric hole site of incubation plate

Abstract

The utility model provides an incubation disc photometric hole site calibration device, and relates to the technical field of biochemical detection, the incubation disc photometric hole site calibration device comprises a chuck used for being detachably connected with an incubation disc, the chuck and the incubation disc rotate synchronously, the chuck is provided with a plurality of calibration white boards used for being in one-to-one correspondence with a plurality of photometric hole sites formed in the incubation disc, and the calibration white boards are arranged on the chuck. An optical module is further fixedly arranged on one side of the incubation plate, the optical module is connected with the processor, the optical module sequentially penetrates through the light metering hole positions to face the calibration white board which sequentially rotates to one side of the optical module to emit light beams, and reflected light reflected by the calibration white board is collected and fed back to the processor so as to obtain optical centers of the multiple light metering hole positions respectively. A calibration white board is installed at each light metering hole position, so that light with different wavelengths can be stably reflected. The multiple light metering hole positions are calibrated at a time, operation is easy, and working efficiency is high. The measurement is accurate, and the accuracy and effectiveness of optical data are improved; the optical module carries out collection through itself, so that the cost is saved; and automatic calibration is carried out in a dark environment, so that the calibration precision is ensured.

Description

Technical Field

[0001] The present application relates to the field of biochemical detection technology, and in particular to a device for calibrating photometric holes in an incubation plate. Background Art

[0002] Dry biochemistry is also called solid-phase biochemistry, which uses a multi-layer thin film solid-phase reagent technology. As long as the liquid sample such as serum, urine, etc. is directly added to the reagent carrier that has been solidified in a special structure, that is, the dry biochemical reagent, the water in the sample is used as the solvent to dissolve the reagent solidified on the carrier, and then react with the components to be tested in the sample to cause the color of the reagent sheet to change. After adding the sample, the reflected light density of the reagent sheet to a specific wavelength is periodically measured within a fixed reaction time, and the reaction curve of the sample during the entire reaction time is drawn. The change value or change rate of the reflected light density is calculated by the reaction curve, and finally the concentration of the analyte in the sample is obtained by the corresponding calibration curve. The detection method of dry biochemistry is reflectance photometry. The color reaction occurs on the solid phase carrier, which has a significant scattering effect on both the transmitted light and the reflected light. It does not follow the Lambert-Beer law, and there are multiple internal reflections between the upper and lower interfaces of the solid phase reaction membrane. The Kubeka-Munk theory is used, or it is corrected by the Williams-Clapper equation.

[0003] In a dry biochemical analyzer, the incubation module and the optical module are important components. The incubation module is designed to provide a constant temperature environment to promote biochemical reactions in biological samples. The optical module is used to measure the reflected light density in the sample for biochemical analysis and detection. The combination of these two modules can achieve rapid and accurate biochemical analysis of biological samples. Accurate optical acquisition is essential to ensure the accuracy and effectiveness of the instrument, which requires close cooperation between the incubation tray and the optical module to ensure that the photometric holes of the incubation tray can perfectly overlap when they are rotated to the photometric position of the optical module.

[0004] There are currently several calibration methods for whether the photometric holes of the incubation plate are aligned with the photometric holes of the optical module, but these methods require additional imaging equipment and are costly; the use of a camera requires manual construction of a shooting platform, and it is difficult to ensure that the optical module and the camera are coaxial, and are easily affected by human factors such as installation; the operation is cumbersome and complicated, and when multiple photometric holes need to be accurately calibrated, it may take longer time, etc., making the overall cost-effectiveness of calibration not meet the requirements. Utility Model Content

[0005] The purpose of the embodiments of the present application is to provide an incubation plate photometric hole position calibration device to achieve automatic calibration, thereby improving the accuracy, effectiveness and efficiency of calibration and saving costs.

[0006] According to one aspect of an embodiment of the present application, there is provided an incubation tray photometric hole calibration device, comprising a chuck for detachably connecting to an incubation tray, the chuck and the incubation tray rotating synchronously, a plurality of calibration white boards being arranged on the chuck for one-to-one correspondence with the plurality of photometric holes arranged on the incubation tray, an optical module being further fixedly arranged on one side of the incubation tray, the optical module being connected to a processor, the optical module sequentially emitting light beams through the photometric holes toward the calibration white boards that sequentially rotate to one side of the optical module, and collecting reflected light reflected by the calibration white boards to feed back to the processor, so as to respectively obtain the optical centers of the plurality of photometric holes.

[0007] Optionally, a plurality of accommodating holes are provided on the chuck, and the calibration whiteboard is arranged in the accommodating holes by a positioning device.

[0008] Optionally, the positioning device includes a retaining ring fixedly disposed on the calibration whiteboard to limit the mechanical position of the calibration whiteboard in the accommodating hole.

[0009] Optionally, an elastic member is further provided between the calibration whiteboard and the accommodating hole, so as to make the calibration whiteboard and the surface of the incubation tray closely attached.

[0010] Optionally, the optical module includes a light source and a collector, the light source emits the light beam toward the calibration white board, and the collector collects the reflected light reflected by the calibration white board.

[0011] Optionally, the reflectivity of the calibration white plate is greater than 80%.

[0012] Optionally, a photoelectric switch is further included, which is connected to the processor. The photoelectric switch is triggered by the rotation of the incubation tray and a plurality of code teeth arranged on the incubation tray, so that the optical module emits and collects the light beam in sequence toward the calibration whiteboard that is rotated to one side of the optical module in sequence, and the code teeth correspond one-to-one to the photometric holes.

[0013] Optionally, the chuck is also provided with a fastener to fasten it to the incubation tray.

[0014] Optionally, the chuck is also provided with a pin hole for positioning with the incubation tray.

[0015] Optionally, a rotating handle is also provided on the chuck.

[0016] The incubation tray photometric hole calibration device provided in the embodiment of the present application has a plurality of calibration whiteboards arranged on the chuck for one-to-one correspondence with the plurality of photometric holes arranged on the incubation tray. The chuck and the incubation tray rotate synchronously and continuously, so that each group of photometric holes and the calibration whiteboard pass through the optical module position in turn. The fixed optical module transmits the light beams through the photometric holes in turn toward the calibration whiteboard that rotates to one side of the optical module in turn, and collects the reflected light reflected by the calibration whiteboard and feeds it back to the processor, so that the optical centers of the plurality of photometric holes can be obtained respectively, and then the deviation between the optical center of each photometric hole and the standard zero center of each photometric hole can be obtained, and the calibration of the photometric holes can be completed. In the subsequent experimental collection, when uploading the data of each photometric hole, only the filtered valid data is uploaded. At this time, the calibrated deviation will be used for offset, so as to improve the accuracy of the experimental data. The incubation tray photometric hole calibration device of the present application adopts a calibration whiteboard installed at each photometric hole, which can stably reflect light of different wavelengths. It can calibrate multiple photometric holes at one time. The integrated design is easy to install and operate, which improves work efficiency and avoids the repetitive and time-consuming process of installing a calibration whiteboard at each photometric hole and then testing them in turn, reducing the tediousness of operation. The measurement is precise, improving the accuracy and effectiveness of optical data; no other optical equipment is required, and the optical module collects data by itself, saving costs; the entire calibration process is carried out in a light-proof environment and is not affected by external environmental factors; automatic calibration is not affected by human factors, ensuring the accuracy of calibration. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 This is one of the partial structural schematic diagrams of the incubation tray photometric hole position calibration device provided in this embodiment;

[0019] Figure 2 This is the second partial structural schematic diagram of the incubation tray photometric hole position calibration device provided in this embodiment;

[0020] Figure 3 This is one of the schematic diagrams of the chuck structure of the incubation plate photometric hole position calibration device provided in this embodiment;

[0021] Figure 4 This is the second schematic diagram of the chuck structure of the incubation plate photometric hole position calibration device provided in this embodiment;

[0022] Figure 5This is the third schematic diagram of the chuck structure of the incubation plate photometric hole position calibration device provided in this embodiment;

[0023] Figure 6 It is a schematic diagram of the structure of an incubation tray used for calibration by the incubation tray photometric hole position calibration device provided in this embodiment;

[0024] Figure 7 It is a schematic diagram of the focus structure of the incubation plate photometric hole position calibration device provided in this embodiment;

[0025] Figure 8 It is a schematic diagram of optical data of a blackboard measured at a certain wavelength by a photometric hole position of an incubation plate photometric hole position calibration device provided in this embodiment;

[0026] Fig. 9 It is a schematic diagram of optical data of the background measured by the photometric hole position of the incubation plate photometric hole position calibration device provided in this embodiment at a certain wavelength;

[0027] Fig.10 It is a schematic diagram of optical data of a white plate measured by the photometric hole positions of the incubation plate photometric hole position calibration device provided in this embodiment at a certain wavelength;

[0028] Fig.11 is a calibration flow chart of the incubation tray photometric hole position calibration device provided in this embodiment;

[0029] Fig.12 This is a schematic diagram of camera-assisted calibration of the incubation tray photometric hole position calibration device provided in this embodiment;

[0030] Fig.13 It is the light spot shape photographed by an industrial camera using the incubation plate photometric hole position calibration device provided in this embodiment.

[0031] Icons: 1-incubation tray photometric hole position calibration device; 11-chuck; 12-calibration whiteboard; 121-positioning device; 122-elastic member; 13-fastener; 14-pin hole; 15-rotating handle; 2-incubation tray; 21-code tooth disk; 211-photometric hole position; 212-code tooth; 3-photoelectric switch; 4-optical module; 41-photometric position; 42-optical detection circuit. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.

[0033] In the description of this application, it should be noted that the terms "inside", "outside", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the application is usually placed when in use, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0034] It should also be noted that, unless otherwise clearly specified and limited, the terms "disposed" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0035] Please refer to Figure 1 , Figure 2 As shown, the embodiment of the present application provides an incubation tray photometric hole calibration device 1 (hereinafter referred to as calibration device 1), which can accurately, quickly and conveniently calibrate the optical center of the photometric hole 211 of the incubation tray 2, and ensure that the optical module 4 can obtain the data of the actual optical center of each photometric hole 211. It includes: a chuck 11 for detachably connecting with the incubation tray 2, the chuck 11 and the incubation tray 2 rotate synchronously, and a plurality of calibration white boards 12 are arranged on the chuck 11, which are used for one-to-one correspondence with the plurality of photometric holes 211 arranged on the incubation tray 2. An optical module 4 is also fixedly arranged on one side of the incubation tray 2, and the optical module 4 is connected to the processor. The optical module 4 sequentially transmits light beams through the photometric holes 211 toward the calibration white board 12 that is rotated to one side of the optical module 4 in turn, and collects the reflected light reflected by the calibration white board 12 and feeds it back to the processor, so as to respectively obtain the optical centers of the plurality of photometric holes 211.

[0036] The incubation tray 2 is provided with a plurality of photometric holes 211, and the chuck 11 is provided with a plurality of calibration white boards 12, which correspond one to one with the photometric holes 211; the incubation tray 2 and the chuck 11 are detachably connected, so that the chuck 11 can be disassembled after the calibration is completed.

[0037] For example, in the present application, the incubation tray 2 is a disc structure. To match the incubation tray 2, the chuck 11 is also a disc structure. Multiple photometric holes 211 are evenly distributed around the circumference of the incubation tray 2, and multiple calibration white boards 12 are evenly distributed around the circumference of the chuck 11. The material of the calibration white boards 12 is uniform, and the color of the photometric surface is uniform.

[0038] The chuck 11 is also provided with a fastener 13 , such as a quick-release screw, which is fastened to the incubation tray 2 to achieve detachability, and the calibration device 1 can be firmly fixed on the incubation tray 2 .

[0039] The chuck 11 is also provided with a pin hole 14 for positioning with the incubation tray 2. The pin hole 14 is used to locate the mechanical position of the calibration device 1 on the incubation tray 2.

[0040] The chuck 11 is also provided with a rotating handle 15 to facilitate the installation and removal of the chuck 11 and the incubation tray 2 .

[0041] An optical module 4 is also provided. During calibration, the chuck 11 and the incubation tray 2 rotate synchronously so that each photometric hole 211 and the corresponding calibration whiteboard 12 rotate to the position of the optical module 4; for example, the optical module 4 is located below the photometric hole 211, and the calibration whiteboard 12 is located above the photometric hole 211. The optical module 4 emits a light beam toward the calibration whiteboard 12 through the photometric hole 211, and the calibration whiteboard 12 reflects the light beam to form reflected light. After the reflected light is received by the optical module 4, it is fed back to the processor. The processor can obtain the optical center of the photometric hole 211 and obtain the deviation between the optical center and the standard zero center of the photometric hole 211.

[0042] The chuck 11 and the incubation tray 2 rotate continuously, and each group of photometric holes 211 and the calibration whiteboard 12 pass through the position of the optical module 4 in turn. The optical module 4 calibrates each photometric hole 211 in turn according to the above method, and the optical center of all the photometric holes 211 on the incubation tray 2 can be obtained, and the deviation of the optical center of each photometric hole 211 and its standard zero center can be obtained.

[0043] The deviation of the above-mentioned photometric hole 211 is recorded. In the subsequent experiment collection through the photometric hole 211 of the incubation plate 2, only the filtered valid data is uploaded when uploading the data of each photometric hole 211. At this time, the calibrated deviation will be used for offset, so that the obtained test data can be more accurate. For example, when the optical center is at the exact center, the middle section of the entire photometric hole 211 data will be uploaded when uploading the data; if the center is biased to the right, the screening value position taken when uploading the data will be offset to the right to the position of the corresponding calibration value, rather than the exact center of the mechanical position.

[0044] Therefore, in the incubation tray photometric hole position calibration device 1 provided in the embodiment of the present application, a plurality of calibration white boards 12 are arranged on the chuck 11, which are used to correspond one-to-one with the plurality of photometric hole positions 211 arranged on the incubation tray 2. The chuck 11 and the incubation tray 2 rotate synchronously and continuously, so that each group of photometric hole positions 211 and the calibration white board 12 pass through the position of the optical module 4 in turn. The optical module 4 emits light beams through the photometric hole positions 211 toward the corresponding calibration white board 12 in turn, and collects the reflected light reflected by the calibration white board 12 and feeds it back to the processor, so that the optical centers of the plurality of photometric hole positions 211 can be obtained respectively, and then the deviation between the optical center of each photometric hole position 211 and the standard zero center of each photometric hole position 211 can be obtained, so as to complete the calibration of the photometric hole position 211. In the collection of subsequent experiments, only the filtered valid data is uploaded when uploading the data of each photometric hole position 211. At this time, the calibrated deviation will be used for offset to improve the accuracy of the experimental data. The incubation plate photometric hole calibration device 1 of the present application adopts a calibration whiteboard 12 installed in each photometric hole 211, which can stably reflect light of different wavelengths. Multiple photometric holes 211 can be calibrated at one time, with an integrated design, easy installation, simple operation, improved work efficiency, avoiding the repetitive and time-consuming process of installing a calibration whiteboard 12 in each photometric hole 211 and then testing in turn, and reducing the cumbersomeness of operation. The measurement is precise, improving the accuracy and effectiveness of optical data; no other optical equipment is required, the optical module 4 collects by itself, saving costs; the entire calibration process is carried out in a light-proof environment and is not affected by external environmental factors; automatic calibration is not affected by human factors, and the accuracy of calibration is guaranteed.

[0045] like Figure 3 to Figure 5 As shown, a plurality of receiving holes are provided on the chuck 11 , and the calibration white plate 12 is arranged in the receiving holes through a positioning device 121 .

[0046] The chuck 11 is provided with a plurality of through accommodating holes, and the calibration white board 12 is located in the accommodating holes. The light beam emitted by the optical module 4 can pass through the photometric hole 211 and be emitted to the calibration white board 12 .

[0047] The calibration whiteboard 12 is disposed in the receiving hole by a positioning device 121 . For example, in one implementation, the positioning device 121 includes a retaining ring fixedly disposed on the calibration whiteboard 12 to limit the mechanical position of the calibration whiteboard 12 in the receiving hole.

[0048] In addition, an elastic member 122 is provided between the calibration whiteboard 12 and the receiving hole, for example, the elastic member 122 can be a spring, so as to make the calibration whiteboard 12 and the surface of the incubation tray 2 close to each other. The elastic member 122 can adapt the height between the surfaces of the incubation tray 2, so that the calibrated whiteboard 12 is close to the surface of the tray.

[0049] In order to ensure that the optical acquisition data is accurate and stable, the calibration should be kept consistent with the actual working conditions to the greatest extent possible to reduce the error caused by the difference between calibration and actual use. The height position of the calibration whiteboard 12 on the calibration device 1 should be ensured to be at the intersection of the incident light path of the optical module 4 and the detection and acquisition light path, such as Figure 7 The height of the calibration white plate 12 installed on the calibration device 1 is calculated by optical design, and the position is designed to be the common focus of multiple wavelengths, and the focal position just intersects with the collection light path.

[0050] The height adjustment is determined by the height of the incubation tray 2. During calibration, firstly, the height of the optical module 4 or the incubation tray 2 is fine-tuned to make the height of the tray at the theoretical design height. The calibration whiteboard 12 installed on the calibration device 1 can be tightly attached to the surface of the incubation tray 2 under the pressure of the elastic member 122, ensuring that the photometric surface of the calibration whiteboard 12 is at the designed intersection position.

[0051] Furthermore, the optical module 4 includes a light source and a collector. The light source emits a light beam toward the calibration whiteboard 12 , and the collector collects reflected light reflected by the calibration whiteboard 12 .

[0052] The optical module 4 can perform two functions: light emission and collection. Figure 6 As shown, the calibration device 1 also includes a photoelectric switch 3, which is connected to the processor. The photoelectric switch 3 is triggered by the rotation of the incubation tray 2 and a plurality of code teeth 212 arranged on the incubation tray 2, so that the optical module 4 emits and collects light beams in sequence toward the calibration whiteboard 12 that is rotated to one side of the optical module 4 in sequence, and the code teeth 212 correspond one-to-one to the photometric hole positions 211.

[0053] For example, a code tooth disk 21 is provided on the incubation disk 2 , and the photometric holes 211 and the code teeth 212 are both provided on the code tooth disk 21 .

[0054] When the incubation tray 2 rotates, the multiple code teeth 212 set on the incubation tray 2 trigger the photoelectric switch 3 in turn to obtain a trigger signal to feed back to the processor, and the processor drives the light source to emit a light beam according to the trigger signal. The code tooth 212 triggers the photoelectric switch 3 once, and the photometric hole position 211 corresponding to the code tooth 212 is calibrated once.

[0055] In addition, the light source can emit light beams of different wavelengths, and the optical center position of the photometric hole 211 is confirmed by optical center position calibration. By closely cooperating with the calibration device 1 and the optical module 4, the light intensity data of multiple wavelengths of each photometric hole 211 on the incubation plate 2 can be dynamically collected at one time, and the optical center of each photometric hole 211 at different wavelengths can be found accurately and quickly.

[0056] When the incubation plate 2 rotates, the detection circuit records the real change data of the reflectivity of the calibration white plate 12 at different wavelengths to form a single-hole photometric light intensity curve. In order to ensure that the curve meets the applicable conditions of the optical peak centering algorithm, it is necessary to ensure that the optical data measured by each photometric hole position 211 has a peak or good symmetry. This is directly related to the selection of the calibration white plate 12 installed on the calibration device 1. The calibration white plates 12 (test objects) with different reflectivities are tested to obtain the following results:

[0057] 1. The curve value measured by the blackboard with low reflectivity is low, the signal-to-noise ratio is low, it is easily affected by interference, and the curve may be asymmetric due to the influence of stray light, such as Figure 8 As shown;

[0058] 2. The curve obtained by directly measuring the background is as follows Fig. 9 As shown, the curve has a trough. Also, due to the influence of stray light around the low-value photometric aperture 211, the symmetry is not good. The center position calculated by using the trough centering has a large deviation from the actual center.

[0059] 3. The curve value of the white board with higher reflectivity is as follows Fig.10 As shown, its light intensity reflection ad is large, the peak is obvious, the light intensity ad at the peak is much larger than the stray light intensity ad around the photometric hole position 211, the signal-to-noise ratio is high, so it will not be affected by stray light or interference, and the curve has good symmetry.

[0060] In summary, the present application uses a calibration white plate 12 with a reflectivity greater than 80% to calibrate the optical center position of the object to be measured.

[0061] The incubation plate photometric hole position calibration device 1 provided in the embodiment of the present application can be calibrated according to the following steps when in use, please refer to Fig.11 :

[0062] (1) Before calibration, confirm that the calibration white plate 12 installed on the calibration device 1 is clean and uniform;

[0063] (2) Insert the calibration device 1 into the fixing pin of the incubation tray 2 according to the pin hole 14, and lock the calibration device 1 and the incubation tray 2 with the fastener 13 on the calibration device 1;

[0064] (3) Use the calibration program (host computer) to calibrate the optical position of the incubation tray 2. The incubation tray 2 keeps rotating at a constant speed after rotating a fixed distance. When the code tooth 212 triggers the photoelectric switch 3, the incubation tray 2 sends a synchronization signal to the optical module 4 every time it moves. The fixed wavelength of light emitted by the optical module 4 is irradiated on the calibration device 1. The light is reflected back to the optical module 4 and collected and stored by the optical detection circuit 42. The optical module 4 records a data each time it receives a synchronization signal. The entire process of each calibration whiteboard 12 passing through the photometric position 41 during rotation is recorded as a set of data. In this embodiment, the optical module 4 turns on the light in order from large to small in wavelength. Each wavelength turns on the light once and passes through twenty photometric holes 211. A total of seven wavelengths pass through a total of one hundred and forty photometric holes 211.

[0065] (4) When the incubation plate 2 completes the calibration operation, the optical module 4 records a total of 140 groups of photometric hole position 211 data of seven wavelengths. Figure 7 The curve is a schematic diagram of a set of photometric aperture 211 data, which is uploaded to the optical peak or trough centering algorithm of the engineering debugging software. The algorithm can simulate a horizontal line at a certain distance below the peak and find the intersection of this horizontal line with the positive half cycle and the negative half cycle. The optical center position of the optical data platform period is determined by calculating the midpoint position of the two intersections. The algorithm can accurately find the optical center position of a total of 140 apertures at seven wavelengths at the corresponding wavelength with high precision.

[0066] (5) The optical center position calibration parameters obtained by the algorithm are stored in the optical module 4.

[0067] (6) Remove the calibration device 1 and complete the calibration.

[0068] After the calibration is completed, the photometric hole 211 of the incubation tray 2 is installed with the calibrated parameters and rotated to the photometric position 41 of the optical module 4. An industrial camera is used to observe the light spot of the photometric hole of the incubation tray 2 irradiated by the optical module 4 at a position coaxial with the incident light path just above the photometric position 41 of the optical module 4, such as Fig.12 The photo of the light spot is shown as Fig.13 As shown, if the light spot is in a concentric position, it means that the calibration position is accurate, and the incubation plate photometric hole position calibration device 1 is accurate and effective.

[0069] The above description is only an embodiment of the present application and is not intended to limit the protection scope of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A device for calibrating photometric holes of an incubation plate, characterized in that: include: A chuck used for detachably connecting with an incubation tray, the chuck and the incubation tray rotate synchronously, a plurality of calibration white boards are arranged on the chuck for one-to-one correspondence with the plurality of photometric holes arranged on the incubation tray, an optical module is also fixedly arranged on one side of the incubation tray, the optical module is connected to a processor, the optical module sequentially transmits light beams through the photometric holes toward the calibration white boards which sequentially rotate to one side of the optical module, and collects reflected light reflected by the calibration white boards and feeds it back to the processor, so as to respectively obtain the optical centers of the plurality of photometric holes.

2. The incubation plate photometric hole position calibration device according to claim 1, characterized in that: The chuck is provided with a plurality of accommodating holes, and the calibration whiteboard is arranged in the accommodating holes through a positioning device.

3. The incubation tray photometry hole position calibration device according to claim 2, characterized in that: The positioning device includes a retaining ring fixedly arranged on the calibration white plate to limit the mechanical position of the calibration white plate in the accommodating hole.

4. The incubation tray photometry hole position calibration device according to claim 2, characterized in that: An elastic member is also provided between the calibration white plate and the accommodating hole, so as to make the calibration white plate and the surface of the incubation plate closely attached.

5. The incubation plate photometric hole position calibration device according to claim 1, characterized in that: The optical module includes a light source and a collector. The light source emits the light beam toward the calibration white board, and the collector collects the reflected light reflected by the calibration white board.

6. The incubation plate photometric hole position calibration device according to claim 1, characterized in that: The reflectivity of the calibration white plate is greater than 80%.

7. The incubation plate photometric hole position calibration device according to any one of claims 1 to 6, characterized in that: It also includes a photoelectric switch, which is connected to the processor. The photoelectric switch is triggered by the rotation of the incubation tray and a plurality of code teeth arranged on the incubation tray, so that the optical module emits and collects the light beam toward the calibration whiteboard that is rotated to one side of the optical module in turn, and the code teeth correspond one-to-one to the photometric holes.

8. The incubation plate photometric hole position calibration device according to claim 1, characterized in that: The chuck is also provided with a fastener to fasten with the incubation tray.

9. The incubation plate photometric hole position calibration device according to claim 1, characterized in that: The chuck is also provided with a pin hole for positioning with the incubation tray.

10. The incubation plate photometric hole position calibration device according to claim 1, characterized in that: The chuck is also provided with a rotating handle.

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