Kit and analyzer
By setting up a tiltable reaction tube placement and permanent magnet in the kit, the problem of solid-phase carrier being sucked away is solved, achieving more accurate and thorough reagent absorption, and improving the reliability of in vitro diagnostic detection.
Patent Information
- Application Number
- CN202323660649.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-29
- Publication Date
- 2024-12-13
- Estimated Expiration
- 2033-12-29
AI Technical Summary
During the in vitro diagnostic detection reagent detection process, solid-phase carriers such as magnetic particles are easily absorbed, resulting in inaccurate test results and incomplete absorption of reaction liquid.
Design a tiltable kit. By setting elastic limits and permanent magnets in the reaction tube placement position, the reaction tube is tilted by external force, and the solid carrier is adsorbed to the tube wall to prevent it from being sucked away, while allowing pipetting. The suction head absorbs the reaction liquid more thoroughly.
It effectively avoids the loss of solid-phase support, improves the accuracy of the detection results and the thorough absorption of the reaction liquid, and ensures the reliability of subsequent testing.
Smart Images
Figure CN222167051U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of in vitro diagnostic testing, in particular to a reagent kit and an analyzer. Background Art
[0002] In vitro diagnostic equipment, especially in the field of clinical immunodiagnosis, test kits are needed to perform tests.
[0003] At present, the testing process of in vitro diagnostic test reagents is roughly as follows: adding samples - incubating and mixing - adding reagents - washing - aspirating the reaction solution - adding reaction reagents - results; and during the action of aspirating the reaction solution, there are solid phase carriers such as magnetic particles that need to be retained in the reaction tube, and the solid phase carriers gathered at the bottom of the tube or near it are easily aspirated, resulting in inaccurate test results, which in turn causes serious consequences.
[0004] In order to avoid the solid phase carrier being sucked away and the problem of incomplete absorption of the reaction solution, it is urgently needed to provide a kit that can help solve this problem. Utility Model Content
[0005] The first purpose of the utility model is to provide a reagent kit which can tilt a reaction tube at a certain angle, and can absorb the magnetic solid phase carrier on the side away from the bottom of the tube with a magnet, so as to prevent the solid phase carrier in the reaction tube from being sucked away.
[0006] The second object of the utility model is to provide an analyzer comprising the above reagent kit.
[0007] In order to achieve the above-mentioned first purpose, the utility model provides a test kit, including a carrier, on which a reaction tube placement position is arranged, the reaction tube placement position includes a placement hole and a position limiting component extending along the hole wall of the placement hole, the position limiting component includes an elastic limiter, and the elastic limiter can be elastically deformed in a direction close to or away from the central axis of the placement hole under the action of an external force, and the elastic limiter recovers its deformation after the external force disappears.
[0008] It can be seen from the above scheme that by setting a placement hole and a position limiting component, one end of the reaction tube is inserted into the position limiting component through the placement hole, so as to facilitate the vertical fixing of the reaction tube; by setting an elastic limiter, when the reaction liquid needs to be sucked away, the elastic limiter is elastically deformed by external force, so that the reaction tube becomes inclined, and then with the cooperation of the external permanent magnet, the solid phase carrier is adsorbed onto the inner wall of one side of the reaction tube to prevent the solid phase carrier from being sucked away. At this time, the pipette tip can be extended to the lowest position at the bottom of the reaction tube, so as to facilitate more thorough suction of the reaction liquid, which is beneficial to improving the accuracy of the subsequent detection structure.
[0009] A further solution is that the elastic limiting member includes first ribs symmetrically arranged in pairs.
[0010] A further solution is that the position limiting assembly further comprises second ribs symmetrically arranged in pairs, and the second ribs are arranged in a direction perpendicular to the direction in which the first ribs are arranged.
[0011] It can be seen from the above solution that by providing two second ribs, when the reaction tube is tilted, the two second ribs can limit the reaction tube from shaking to both sides.
[0012] A further solution is that the carrier is also provided with at least one of a reaction liquid storage tank, a pipette tip placement position and a reagent placement position.
[0013] A further solution is that the reaction tube placement position is equipped with a reaction tube, the pipette tip placement position is equipped with a pipette tip, and the reagent placement position is equipped with a reagent tube.
[0014] A further solution is that the carrier is also provided with a reaction liquid storage tank, a pipette tip placement position and a reagent placement position, and the reaction liquid storage tank, the reagent placement position and the pipette tip placement position are all arranged in a straight line on one side of the reaction tube placement position.
[0015] As can be seen from the above scheme, by arranging the reaction liquid storage tank, the reagent placement position and the pipette tip placement position all in a straight line on one side of the reaction tube placement position, the reagent kit is made to have a rectangular structure as a whole, which facilitates the orderly placement of multiple reagent kits and saves space. In addition, the reaction liquid storage tank, the reagent placement position and the pipette tip placement position will not affect the external force acting on the reaction tube placement position.
[0016] A further solution is that the kit also includes an installation unit.
[0017] A further solution is that the mounting portion includes a first mounting portion and a second mounting portion which are arranged on opposite sides of the test kit, the first mounting portion extends from the carrier and forms an avoidance groove between the first mounting portion and the carrier, the first mounting portion can be elastically tilted toward the direction approaching the second mounting portion, and the extending end of the first mounting portion is provided with a hook portion protruding away from the avoidance groove; the second mounting portion includes a protruding structure protruding in the direction away from the first mounting portion, and the connecting surface between the protruding structure and the side surface of the carrier is an inclined surface.
[0018] A further solution is that the carrier further includes an anti-slip portion.
[0019] In order to achieve the above second purpose, the analyzer provided by the utility model includes a reagent box and a mounting seat for mounting the reagent box, wherein a mounting groove is arranged in the mounting seat, and a stopper and a clamping groove are respectively arranged on opposite sides of the mounting groove.
[0020] The reagent kit of the utility model can vertically fix the reaction tube under normal conditions. When pipetting is required, the reaction tube can be driven to an inclined state by external force, so that the reaction liquid can be more thoroughly sucked from the lowest position of the reaction tube. The loss of the solid phase carrier can be avoided by magnetic attraction between the external permanent magnet and the solid phase carrier in the reaction tube, thereby ensuring the effective progress of the reaction. Since the elastic limiter can be elastically deformed, when the pipetting is completed, the elastic limiter can drive the reaction tube to return to a vertical state, so that the subsequent addition of reactants is convenient and sufficient reaction can be achieved in the reaction tube. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 It is a three-dimensional structural diagram of an embodiment of the test kit of the utility model.
[0022] Figure 2 It is a schematic diagram of the top view of the structure of the position limiting component at the placement position of the reaction tube in the embodiment of the reagent kit of the utility model.
[0023] Figure 3 It is a cross-sectional view of an embodiment of the test kit of the present utility model.
[0024] Figure 4 It is a working schematic diagram of the embodiment of the kit of the utility model when aspirating the reaction solution.
[0025] Figure 5 It is a cross-sectional schematic diagram of the connection between the reagent kit embodiment of the utility model and the mounting seat.
[0026] Reference numerals:
[0027] 1-carrier, 11-reaction tube placement position, 111-placement hole, 112-position limiting component, 1121-first rib, 1122-second rib, 11221-raised portion, 12-reaction liquid storage tank, 13-pipette tip placement position, 14-reagent placement position, 15-first mounting portion, 151-stop surface, 16-second mounting portion, 161-inclined surface, 17-avoidance groove, 18-anti-slip portion;
[0028] 2- reaction tube;
[0029] 3-Pipette tips;
[0030] 4-Reagent tube;
[0031] 5-mounting seat, 51-mounting slot, 511-stopper, 512-slot;
[0032] 6- Permanent magnet.
[0033] The present invention will be further described below with reference to the accompanying drawings and embodiments. DETAILED DESCRIPTION
[0034] Kit Example:
[0035] See also Figure 1 A reagent kit provided in this embodiment includes a carrier 1, on which a reaction tube placement position 11, a reaction liquid storage tank 12, a pipette tip placement position 13 and a reagent placement position 14 are provided. The reaction tube placement position 11 is used to hold the reaction tube 2, the reaction liquid storage tank 12 is used to store the reaction waste liquid, the pipette tip placement position 13 is used to hold the pipette tip 3, and the reagent placement position 14 is used to hold various reagents, including but not limited to: cleaning solution, magnetic particles, enzymes, etc.
[0036] The reaction tube placement position 11, the reaction liquid storage tank 12, the reagent placement position 14 and the pipette tip placement position 13 are all arranged in a straight line, so that the carrier 1 is a rectangular parallelepiped as a whole, which is convenient for multiple reagent kits to be arranged side by side, which is conducive to saving space. The number of reagent placement positions 14 and pipette tip placement positions 13 in the carrier 1 is set according to the number of reagents and consumables required for the reaction, so that each reagent kit contains all the materials required for the reaction, which is convenient to use.
[0037] Furthermore, the reaction liquid storage tank 12 and the reagent placement position 14 are both arranged between the pipette tip placement position 13 and the reaction tube placement position 11, and the reaction liquid storage tank 12 is arranged adjacent to the reaction tube placement position 11, which can improve work efficiency. In this embodiment, the number of the pipette tip placement position 13 and the reagent placement position 14 are both set to multiple, and the multiple reagent placement positions 14 are arranged adjacent to each other.
[0038] The reaction tube placement position 11 includes a placement hole 111 and a position limiting component 112 extending along the hole wall of the placement hole 111. The position limiting component 112 includes an elastic limiting component. The elastic limiting component can be elastically deformed in a direction close to or away from the central axis of the placement hole under the action of an external force, and the elastic limiting component recovers its deformation after the external force disappears.
[0039] Preferably, combined Figures 1 to 4 The position defining components 112 are disposed at the lower part of the placement hole 111, and a placement cavity communicating with the placement hole 111 is formed between the position defining components 112. The reaction tube 2 is detachably disposed in the placement hole 111 and the placement cavity.
[0040] The wall of the placement hole 111 can extend in the vertical direction or extend obliquely in the up-down direction to form a limiting component 112. Preferably, a plurality of elastic stoppers are arranged along the wall of the placement hole 111 obliquely toward the central axis of the placement hole 111, so that the width of the placement cavity can be variable. Before the reaction tube 2 is placed in the placement cavity, the width of the placement cavity is the smallest; after the reaction tube 2 is placed in the placement cavity, the reaction tube 2 opens the elastic stoppers, so that the elastic stoppers are always in contact with the outer wall of the reaction tube 2, so as to fix the reaction tube 2 and prevent the reaction tube 2 from accidentally leaving the placement cavity.
[0041] The external force may directly act on the elastic limiting member to deform it, or may act on the reaction tube 2 placed in the placement hole 111 to push the reaction tube 2 so that the reaction tube 2 is tilted and the elastic limiting member is deformed.
[0042] In this embodiment, the upper end of the reaction tube 2 protrudes a placement hole 111. Figure 4 As shown, when the external force F pushes the reaction tube 2 to the right from the upper part of the reaction tube 2, the lower part of the reaction tube 2 rotates to the left, so that the placement cavity can extend outwardly at an angle to accommodate the reaction tube 2 at an angle. When the reaction tube 2 is in an inclined state, the magnetic particles in the reaction tube 2 are magnetically attracted from the right side by the permanent magnet 6, so that the magnetic particles are adsorbed on the side away from the lowest position of the bottom of the reaction tube 2; at this time, the pipette tip 3 extends into the lowest position of the bottom of the reaction tube 2, which can more thoroughly absorb the reaction liquid, avoid absorbing the magnetic particles, and reduce the loss of the magnetic particles.
[0043] Preferably, the elastic limiting members are symmetrically arranged in pairs, which is beneficial to the limiting effect on the reaction tube 2.
[0044] Specifically, the elastic stopper includes a first rib plate 1121 symmetrically arranged in pairs, and the position limiting assembly also includes a second rib plate 1122 symmetrically arranged in pairs, and the direction in which the second rib plate 1122 is arranged is perpendicular to the direction in which the first rib plate 1121 is arranged. The second rib plate 1122 can be an elastic stopper or a non-elastic stopper. In this embodiment, two first rib plates 1121 and two second rib plates 1122 are taken as an example, and the second rib plate 1122 is a non-elastic stopper. The two first rib plates 1121 are relatively arranged on both sides of the first direction of the placement cavity, and the two second rib plates 1122 are relatively arranged on both sides of the second direction of the placement cavity. In this embodiment, the first direction is preferably the length direction of the carrier 1, and the second direction is preferably the width direction of the carrier 1. A placement cavity is formed between the two first rib plates 1121 and the two second rib plates 1122, so that the placement cavity has four cavity walls, which can limit the position of the reaction tube 2 from all sides, and prevent the reaction tube 2 from shaking during the reaction, affecting the detection efficiency and results.
[0045] The first rib 1121, the second rib 1122 and the carrier 1 are integrally formed. The first rib 1121 can be elastically swung toward the inside and outside of the placement cavity, so that the placement cavity can fix the reaction tube 2 therein to prevent the reaction tube 2 from accidentally leaving the placement cavity, and when the reaction tube 2 is tilted, the first rib 1121 is elastically deformed, and the two second ribs 1122 can limit the reaction tube 2 from swinging in other directions.
[0046] Preferably, the lower ends of the reaction tube 2 protrude from the bottom of the placement chamber.
[0047] Specifically, the first rib 1121 constitutes the first cavity wall of the placement cavity, and the first rib 1121 extends downward from the top wall of the carrier 1 by a distance L1, and the distance L1 is smaller than the distance L2 from the top wall of the carrier 1 to the bottom of the reaction tube 2, that is, the bottom of the reaction tube 2 protrudes out of the placement cavity, so that the external permanent magnet 6 can be adjacent to the lower peripheral wall of the reaction tube 2 to magnetically attract the magnetic particles in the reaction tube 2.
[0048] The second rib 1122 constitutes the second cavity wall of the placement cavity, and the second rib 1122 extends vertically downward from the top wall of the carrier 1, and the bottom surface of the second rib 1122 and the bottom surface of the first rib 1121 are preferably in the same horizontal plane. Figure 2 In the embodiment, the width of the second rib 1122 is greater than that of the first rib 1121, so that when the reaction tube 2 tilts toward one side of the first rib 1121, the reaction tube 2 is not easy to move in the direction where the two second ribs 1122 are located, that is, the reaction tube 2 only moves in the direction where the first rib 1121 is located.
[0049] Furthermore, protrusions 11221 are provided on both sides of the second rib 1122 in the width direction. The protrusions 11221 protrude from the inner surface of the second rib 1122 to limit the movement of the reaction tube 2 between the two protrusions 11221 .
[0050] Furthermore, the carrier 1 is also provided with a mounting portion for mounting the carrier 1 .
[0051] Combination Figure 3 and Figure 5 In this embodiment, the mounting parts of the carrier 1 are arranged on two opposite sides thereof. Specifically, a first mounting part 15 and a second mounting part 16 are respectively arranged on both sides in the length direction thereof, and the first mounting part 15 and the second mounting part 16 are arranged on the lower side of the carrier 1 opposite to each other. The first mounting part 15 and the second mounting part 16 are integrally formed with the carrier 1.
[0052] In this embodiment, the carrier 1 is provided with a first mounting portion 15 on the side thereof close to the reaction tube placement position 11. The first mounting portion 15 extends from the carrier 1 and forms an escape groove 17 with the carrier 1. The first mounting portion 15 can be elastically tilted toward the second mounting portion 16 into the escape groove 17. The extended end of the first mounting portion 15 is provided with a hook portion protruding away from the escape groove 17. The hook portion is provided with a stop surface 151 for limiting the upward movement of the carrier 1. The stop surface 151 is used to abut against a stop portion 511 in the mounting seat 5 of the peripheral device to prevent the reagent kit from being accidentally separated from the mounting seat 5.
[0053] The second mounting portion 16 is arranged on the other side of the carrier 1 relative to the first mounting portion 15. The second mounting portion 16 includes a protruding structure protruding in a direction away from the first mounting portion 15. The protruding structure protrudes from the side of the carrier 1. The connecting surface between the protruding structure and the side of the carrier 1 is an inclined surface, that is, the second mounting portion 16 is provided with inclined surfaces 161 on its upper and lower sides, and the inclined surface 161 has a smooth transition with the side of the carrier 1.
[0054] In order to effectively grab the reagent kit, the carrier 1 is also provided with an anti-slip portion 18, such as Figure 1 In this embodiment, an anti-slip portion 18 is preferably provided on both sides of the width direction of the carrier 1. The anti-slip portion 18 is provided close to the second mounting portion 16. The anti-slip portion 18 includes a plurality of ribs, which protrude from the surface of the carrier 1, are arranged along the length direction of the carrier 1, and extend along the height direction of the carrier 1.
[0055] Preferably, the carrier 1 is integrally formed and is provided with a reaction tube placement position 11 , a reaction liquid storage tank 12 , a pipette tip placement position 13 , a reagent placement position 14 , a mounting portion and an anti-slip portion 18 .
[0056] The reagent kit includes an integrally formed carrier 1, a reaction tube 2 is correspondingly installed in the reaction tube placement position 11, a reagent tube 4 is correspondingly installed in the reagent placement position 14, and a pipette tip 3 is correspondingly installed in the pipette tip placement position 13. The reagent tube 4 can be pre-filled with the required reagent solution and packaged, and the reaction tube 2 and the pipette tip 3 are also pre-packaged with the carrier 1 as a whole to avoid contamination and facilitate subsequent use.
[0057] Analyzer Example:
[0058] See also Figure 5 The analyzer includes a reagent box and a mounting base 5 for mounting the reagent box.
[0059] Specifically, the mounting seat 5 is provided with a mounting groove 51, and the opposite sides of the mounting groove 51 are respectively provided with a stopper 511 and a clamping groove 512, the stopper 511 matches the shape of the hook of the first mounting portion 15, and the clamping groove 512 matches the shape of the second mounting portion 16. The lower part of the reagent box is detachably arranged in the mounting groove 51, the first mounting portion 15 is matched with the stopper 511, and the second mounting portion 16 is matched with the clamping groove 512.
[0060] When the reagent box needs to be taken out, the reagent box can be moved to the left by a certain distance, so that the first mounting portion 15 is elastically deformed, so that the second mounting portion 16 is separated from the slot 512 first, and then the first mounting portion 15 is separated from the stop portion 511, and the reagent box can be taken out.
[0061] Working principle: During the pipetting process, the sample solution is transferred to the reaction tube 2 pre-filled with magnetic particles through the pipetting tip 3. After use, the pipetting tip 3 is placed back in the pipetting tip placement position 13; after the reaction in the reaction tube 2 is completed, the reaction tube 2 can be tilted under the action of the external force F, and at the same time, the permanent magnet 6 approaches and adsorbs the magnetic particles on the tube wall on the side facing away from the lowest position of the bottom of the reaction tube 2; at this time, the pipetting tip 3 vertically enters the reaction tube 2 and absorbs the reaction liquid at the lowest position of the reaction tube 2, which is convenient for the reaction liquid to be completely absorbed. Since the absorption position of the pipetting tip 3 is far away from the magnetic particle adsorption position, the loss of magnetic particles can be effectively avoided; the pipetting tip 3 stores the absorbed reaction liquid in the reaction liquid storage tank 12, and the pipetting tip 3 is placed back to its original position after use; after the absorption is completed, the external force F disappears, the permanent magnet 6 moves away, and under the elastic action of the first rib 1121, the reaction tube 2 returns to its initial position, that is, the vertical position. By analogy, the required reagents are pipetted, added, and discarded until the reaction is completed. After the reaction is completed, the test solution is transferred to other components of the analyzer, and the entire kit can be discarded, which is convenient to use.
[0062] In summary, the utility model provides a reagent kit that can be tilted after the reaction tube is placed, and the elastic limiting piece of the placement cavity can conveniently fix the reaction tube so that the reagent can react in the reaction tube; when the reaction liquid needs to be sucked away, the reaction tube is pushed by external force, which can elastically deform the elastic limiting piece that limits the reaction tube, so that the reaction tube becomes tilted, and then with the cooperation of the external permanent magnet, the magnetic particles are adsorbed onto the inner wall of one side of the reaction tube to prevent the magnetic particles from being sucked away. At this time, the pipette tip can be extended to the lowest position at the bottom of the reaction tube, which is convenient for more thorough suction of the reaction liquid, avoiding the loss of magnetic particles, and ensuring the effective progress of the reaction.
[0063] Finally, it should be emphasized that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various changes and modifications. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A kit, comprising a carrier, characterized in that: A reaction tube placement position is provided on the carrier, and the reaction tube placement position includes a placement hole and a position limiting component extending along the hole wall of the placement hole. The position limiting component includes an elastic limiting component. The elastic limiting component can be elastically deformed in a direction close to or away from the central axis of the placement hole under the action of an external force, and the elastic limiting component recovers its deformation after the external force disappears.
2. The kit according to claim 1, characterized in that: The elastic limiting member includes first ribs symmetrically arranged in pairs.
3. The kit according to claim 2, characterized in that: The position limiting assembly further includes second ribs symmetrically arranged in pairs, wherein the second ribs are arranged in a direction perpendicular to the direction in which the first ribs are arranged.
4. The kit according to claim 3, characterized in that: The carrier is also provided with at least one of a reaction liquid storage tank, a pipette tip placement position and a reagent placement position.
5. The kit according to claim 4, characterized in that: The reaction tube placement position is equipped with a reaction tube, the pipette tip placement position is equipped with a pipette tip, and the reagent placement position is equipped with a reagent tube.
6. The kit according to claim 4, characterized in that: The carrier is also provided with a reaction liquid storage tank, a pipette tip placement position and a reagent placement position. The reaction liquid storage tank, the reagent placement position and the pipette tip placement position are all arranged in a straight line on one side of the reaction tube placement position.
7. The kit according to any one of claims 1 to 6, characterized in that: The kit also includes a mounting portion.
8. The kit according to claim 7, characterized in that: The mounting portion includes a first mounting portion and a second mounting portion disposed on opposite sides of the reagent box, the first mounting portion extending from the carrier and forming an avoidance groove between the carrier, the first mounting portion being elastically inclined toward the direction approaching the second mounting portion, and a hook portion protruding away from the avoidance groove being disposed at the extending end of the first mounting portion; The second mounting portion includes a protruding structure protruding in a direction away from the first mounting portion, and a connecting surface between the protruding structure and the side surface of the carrier is an inclined surface.
9. The kit according to claim 8, characterized in that: The carrier also includes an anti-slip portion.
10. An analyzer, characterized in that: It comprises the reagent kit as claimed in any one of claims 1 to 9 and a mounting seat for mounting the reagent kit, wherein a mounting groove is arranged in the mounting seat, and a stopper and a clamping groove are respectively arranged on opposite sides of the mounting groove.