Ultra-efficient competent cell preparation kit

Abstract

The utility model discloses an ultra-efficient competent cell preparation kit which comprises a base, fixing assemblies are symmetrically arranged on the two sides of the base, a driving motor is arranged in the base, a rotating shaft is arranged at the output end of the driving motor, a rotating rod is arranged at the top of the rotating shaft, a push rod is arranged at the top of the rotating rod, and the push rod is arranged on the top of the rotating rod. A mounting seat is arranged at the bottom in the base; under the cooperation of the connecting frame, the rotating shaft, the driving motor, the push rod, the rotating rod, the mounting seat, the connecting rod and the sliding plate, in the preparation process, the protective shell can be quickly driven to reciprocate, and the mixing speed is accelerated under the action of shaking force, so that the preparation efficiency is improved, the experiment progress is accelerated, and the experiment cost is reduced. And under the cooperation of a plug plate and a top plate, a plurality of groups of test tubes can be sealed at the same time in the process of placing the test tubes, and the condition of reagent spilling is avoided in the preparation process.

Description

Technical Field

[0001] The utility model relates to the technical field of kits, in particular to a super-efficient competent cell preparation kit. Background Art

[0002] Competent cells are cells that are induced by physical and chemical methods to be in the most suitable physiological state for taking up and accommodating foreign DNA. In the process of cell preparation, in order to ensure the efficiency of preparation and the safety of storage, the kit is needed for preservation;

[0003] The existing authorization publication number is "CN211227114U" and is a super-efficient competent cell preparation kit, which includes a box body, a switch is fixedly installed at the right end of the box body, a support leg is fixedly connected to the lower end of the box body, the box body and the support leg are an integrated structure, a battery is fixedly installed at the lower end of the box body, a through hole is provided at the lower end of the box body, a motor is fixedly installed at the lower end of the box body, a rotating shaft is provided at the upper end of the motor, a square block is provided at the upper end of the rotating shaft, a bottom plate is clamped at the upper end of the square block, a square groove is provided at the lower end of the bottom plate, and a square block is clamped in the square groove. This device can make the reagent tube move in the reagent box, which will be mixed more quickly and evenly, and the competent cell preparation speed is fast, which saves experimental time and improves experimental efficiency. At the same time, reagent tubes of different models and sizes can be placed, which improves the utilization rate of the reagent box and avoids insufficient use of the reagent box.

[0004] However, during the preparation process, the device simply uses centrifugal force to mix the reagents, which is inefficient and requires a long time to work to ensure that the reagents are mixed. In addition, the device is not fixed during the working process, which causes the device to shake randomly, causing damage to the stored test tubes. Utility Model Content

[0005] The purpose of the utility model is to provide a super-efficient competent cell preparation kit to solve the existing problems raised in the above-mentioned background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an ultra-high efficiency competent cell preparation kit, comprising a base, fixing components are symmetrically provided on both sides of the base, a driving motor is provided inside the base, a rotating shaft is provided at the output end of the driving motor, a rotating rod is provided on the top of the rotating shaft, a push rod is provided on the top of the rotating rod, a mounting seat is provided at the bottom inside the base, connecting rods are symmetrically provided on both sides of the top of the mounting seat, a slide is slidably connected inside the connecting rod, a connecting frame is provided between two groups of the slides, a connecting rod is provided on one side of the top of the slide, a protective shell is provided between the tops of the two groups of the connecting rods, a placing seat is provided at the bottom inside the protective shell, a plurality of groups of test tube placing slots are evenly provided on the top of the placing seat, an extension frame is provided on the top of the test tube placing slot, a top plate is provided on the top of the protective shell, and a plurality of groups of plug plates are evenly provided on the bottom of the top plate.

[0007] Preferably, the fixing assembly includes a mounting plate, a movable rod, a slide groove, an upper clamping plate, a spring, a sliding rod and a lower clamping plate. The mounting plates are located on both sides of the base, a slide groove is provided on the top of the mounting plate, a movable rod is slidably connected inside the slide groove, an upper clamping plate is provided at the bottom of the movable rod, sliding rods are symmetrically provided at both ends of the bottom of the upper clamping plate, a lower clamping plate is provided at the bottom of the sliding rod, and a spring is provided on the outside of the sliding rod. During the preparation process, the device can be fixed at a specified position. During the preparation process, the device will not be affected by the vibration force and move at will, thereby ensuring the stability of the device during placement.

[0008] Preferably, a mounting groove is provided inside the base, and the drive motor is located inside the mounting groove, so that the drive motor is fixed to ensure the working stability of the drive motor during the starting process.

[0009] Preferably, a mounting hole is provided at the bottom of the upper clamping plate, the sliding rod is located inside the mounting hole, and the spring is respectively connected to the upper clamping plate and the lower clamping plate to facilitate the upper and lower movement of the lower clamping plate and prevent its movement from being affected.

[0010] Preferably, limiting rings are symmetrically provided at the top and bottom of the outer side of the moving rod, and the diameter of the limiting rings is larger than the width of the sliding groove, so as to limit the position of the moving rod and prevent it from falling.

[0011] Preferably, a through hole is provided at the mounting seat, and the top of the rotating shaft is connected to the rotating rod along the through hole to facilitate the connection.

[0012] Compared with the prior art, the utility model has the following beneficial effects: the ultra-high efficiency competent cell preparation kit;

[0013] 1. Through the cooperation of the connecting frame, the rotating shaft, the driving motor, the push rod, the rotating rod, the mounting seat, the connecting rod and the slide plate, during the preparation process, the protective shell can be quickly driven to reciprocate, and the shaking force can be used to speed up the mixing speed, thereby improving the preparation efficiency and the progress of the experiment. In addition, through the cooperation of the plug plate and the top plate, multiple groups of test tubes can be sealed at the same time during the test tube placement process, thereby preventing the reagent from spilling during the preparation process.

[0014] 2. Through the cooperation of the mounting plate, the moving rod, the slide groove, the upper clamping plate, the spring, the slide rod and the lower clamping plate, the device can be fixed at the designated position during the preparation process. During the preparation process, the device will not be affected by the vibration force and move at will, thereby ensuring the stability of the device during placement and preventing the test tube from being damaged. At the same time, it is also convenient for the subsequent disassembly and replacement of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional diagram of the utility model;

[0016] Figure 2 It is a front cross-sectional view of the utility model;

[0017] Figure 3 It is the front view of the utility model;

[0018] Figure 4 It is a top view of the mounting seat of the utility model;

[0019] Figure 5 It is a structural diagram of the fixing component of the utility model.

[0020] In the figure: 1. base; 2. connecting frame; 3. rotating shaft; 4. driving motor; 5. pushing rod; 6. rotating rod; 7. mounting seat; 8. fixing assembly; 81. mounting plate; 82. moving rod; 83. sliding groove; 84. upper clamping plate; 85. spring; 86. sliding rod; 87. lower clamping plate; 9. placing seat; 10. test tube placing groove; 11. protective shell; 12. extension frame; 13. plug plate; 14. top plate; 15. connecting rod; 16. sliding plate. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1-Figure 5The utility model provides an embodiment of a super-efficient competent cell preparation kit, comprising a base 1, two sides of the base 1 are symmetrically provided with fixing components 8, the fixing components 8 include a mounting plate 81, a moving rod 82, a slide groove 83, an upper clamping plate 84, a spring 85, a slide rod 86 and a lower clamping plate 87, the mounting plate 81 is located on both sides of the base 1, the top of the mounting plate 81 is provided with a slide groove 83, the inside of the slide groove 83 is slidably connected with the moving rod 82, the top and bottom of the outer side of the moving rod 82 are symmetrically provided with limiting rings, and the diameter of the limiting rings is greater than the width of the slide groove 83, the position of the moving rod 82 is limited to prevent When it falls, an upper clamping plate 84 is provided at the bottom of the moving rod 82, and sliding rods 86 are symmetrically provided at both ends of the bottom of the upper clamping plate 84, and a lower clamping plate 87 is provided at the bottom of the sliding rod 86. A spring 85 is provided on the outer side of the sliding rod 86, and a mounting hole is provided at the bottom of the upper clamping plate 84. The sliding rod 86 is located inside the mounting hole. The spring 85 is connected to the upper clamping plate 84 and the lower clamping plate 87 respectively, so as to facilitate the lower clamping plate 87 to move up and down and prevent its movement from being affected. During the preparation process, the device can be fixed at a specified position. During the preparation process, the device will not be affected by the vibration force and move at will, so as to ensure the stability of the device during placement.

[0023] A driving motor 4 is provided inside the base 1, and a mounting groove is provided inside the base 1. The driving motor 4 is located inside the mounting groove, and the driving motor 4 is fixed. During the starting process of the driving motor 4, the working stability of the driving motor 4 is ensured. A rotating shaft 3 is provided at the output end of the driving motor 4, and a rotating rod 6 is provided at the top of the rotating shaft 3. A push rod 5 is provided at the top of the rotating rod 6. A mounting seat 7 is provided at the bottom inside the base 1, and a through hole is provided at the mounting seat 7. The top of the rotating shaft 3 is connected to the rotating rod 6 along the through hole to facilitate its connection. Connecting rods 15 are symmetrically provided on both sides of the top of the mounting seat 7. A slide plate 16 is slidably connected inside the connecting rod 15. A connecting frame 2 is provided between the two groups of slide plates 16. A connecting rod 15 is provided on one side of the top of the slide plate 16. A protective shell 11 is provided between the tops of the two groups of connecting rods 15. A placing seat 9 is provided at the bottom inside the protective shell 11. A plurality of test tube placing grooves 10 are evenly provided on the top of the placing seat 9. An extension frame 12 is provided on the top of the test tube placing groove 10. A top plate 14 is provided on the top of the protective shell 11, and a plurality of plug plates 13 are evenly provided on the bottom of the top plate 14.

[0024] Working principle: During the use of the device, first place the device at the designated position, then pull the moving rod 82 to move, drive the upper clamping plate 84 to move to the designated position, and then pull the lower clamping plate 87 to move downward, then continue to move the upper clamping plate 84, and release the lower clamping plate 87 after the movement is completed. At this time, the spring 85 resets and drives the slide bar 86 and the lower clamping plate 87 to move upward, thereby clamping at the edge position of the seat surface to complete the fixation of the device. Then, during the preparation process, first, multiple groups of test tubes are placed in the extension frame 12 in sequence, and then the top plate 14 is covered on the top of the protective shell 11, and the plug plate 13 is inserted into the inside of the test tube to seal it to a certain extent. After the sealing is completed, the drive motor 4 is started, and the drive motor 4 drives the rotating shaft 3, the rotating rod 6 and the push rod 5 to rotate. During the rotation process, the push rod 5 pushes the connecting frame 2 to reciprocate, thereby driving the slide plate 16 to move, and the slide plate 16 drives the connecting rod 15 and the protective shell 11 to move. Then, during the movement, the cell reagents in the test tubes inside the protective shell 11 are shaken, thereby speeding up the preparation speed.

[0025] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0026] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0027] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

Claims

1. A kit for preparing ultra-high efficiency competent cells, comprising a base (1), Features: The base (1) is symmetrically provided with fixing components (8) on both sides, the base (1) is provided with a driving motor (4) inside, the output end of the driving motor (4) is provided with a rotating shaft (3), the top of the rotating shaft (3) is provided with a rotating rod (6), the top of the rotating rod (6) is provided with a push rod (5), the bottom of the base (1) is provided with a mounting seat (7), the top of the mounting seat (7) is symmetrically provided with connecting rods (15) on both sides, the interior of the connecting rod (15) is slidably connected with a slide plate (16), and two groups of the slide plates (16) are provided. A connecting frame (2) is provided between the slide plates (16), a connecting rod (15) is provided on one side of the top of the slide plate (16), a protective shell (11) is provided between the tops of the two groups of connecting rods (15), a placement seat (9) is provided at the bottom inside the protective shell (11), a plurality of groups of test tube placement slots (10) are evenly provided on the top of the placement seat (9), an extension frame (12) is provided on the top of the test tube placement slots (10), a top plate (14) is provided on the top of the protective shell (11), and a plurality of groups of plug plates (13) are evenly provided on the bottom of the top plate (14).

2. The ultra-high efficiency competent cell preparation kit according to claim 1, Features: The fixing assembly (8) comprises a mounting plate (81), a moving rod (82), a slide groove (83), an upper clamping plate (84), a spring (85), a sliding rod (86) and a lower clamping plate (87). The mounting plate (81) is located on both sides of the base (1). A slide groove (83) is provided on the top of the mounting plate (81). The moving rod (82) is slidably connected inside the slide groove (83). An upper clamping plate (84) is provided at the bottom of the moving rod (82). Sliding rods (86) are symmetrically provided at both ends of the bottom of the upper clamping plate (84). A lower clamping plate (87) is provided at the bottom of the sliding rod (86). A spring (85) is provided on the outer side of the sliding rod (86).

3. The ultra-high efficiency competent cell preparation kit according to claim 1, Features: The base (1) is provided with a mounting groove inside, and the drive motor (4) is located inside the mounting groove.

4. The ultra-high efficiency competent cell preparation kit according to claim 2, Features: A mounting hole is provided at the bottom of the upper clamping plate (84), the sliding rod (86) is located inside the mounting hole, and the spring (85) is connected to the upper clamping plate (84) and the lower clamping plate (87) respectively.

5. The ultra-high efficiency competent cell preparation kit according to claim 2, Features: Limiting rings are symmetrically provided at the top and bottom of the outer side of the moving rod (82), and the diameter of the limiting rings is greater than the width of the sliding groove (83).

6. The ultra-high efficiency competent cell preparation kit according to claim 1, Features: A through hole is provided at the mounting seat (7), and the top of the rotating shaft (3) is connected to the rotating rod (6) along the through hole.

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