Novel tissue culture seedling culture air circulation device

Abstract

The utility model relates to a novel tissue culture seedling culture air circulation device which comprises a box body, a plurality of layers of culture plates used for placing culture trays are installed in the box body through partition plates on the two sides and a partition plate in the middle, and the air circulation device comprises a temperature regulation and control module, a humidity regulation and control module, a carbon dioxide concentration regulation and control module and a controller which are installed on the box body. And a temperature and humidity sensor and a carbon dioxide sensor which are electrically connected with the controller are also arranged in the box body. According to the utility model, a suitable growth environment can be provided for the culture of tissue culture seedlings, the culture period is shortened, and the culture efficiency and quality are improved.

Description

Technical Field

[0001] The utility model relates to an air circulation device, in particular to a novel tissue culture seedling cultivation air circulation device, belonging to the technical field of biological cultivation devices. Background Art

[0002] Ginseng fruit was originally called melon eggplant, also known as fragrant pear and beautiful fruit. It has the characteristics of high protein, low fat and low sugar. It is rich in calcium, vitamin C, molybdenum, cobalt, selenium and dozens of trace elements needed by the human body, especially selenium and calcium. The content is much higher than other fruits and vegetables. "With the continuous expansion of ginseng fruit planting area and the in-depth development of the industry, the demand for high-quality seedlings has increased rapidly. It is of great significance to carry out research on ginseng fruit seedling breeding technology, promote high-quality seedlings, and standardize seed use patterns for the cultivation and development of ginseng fruit.

[0003] At present, the propagation of ginseng fruit mostly adopts conventional tissue culture, which uses agar as a support in a sealed culture bottle, and adds sucrose to the culture medium for plant growth for rapid propagation. Although the conventional tissue culture method can quickly produce a large number of ginseng fruit seedlings, the culture medium contains a large amount of sugar, which is easy to cause microbial contamination during the entire tissue culture production process, so that the plants cannot grow normally or even die, resulting in a large loss of manpower and material resources. In addition, due to the use of a small sealed container with high humidity and weak light, the tissue culture seedlings grow thin and weak, the seedling quality is relatively poor, and the mortality rate is high during the seedling hardening period.

[0004] Compared with conventional tissue culture, sugar-free tissue culture technology uses carbon dioxide instead of sugar as the carbon source for plants, regulates the growth and rooting development of tissue culture seedlings through environmental adjustment, does not require high airtightness of the container, and uses relatively cheap vermiculite as the support. The key is that the survival rate of transplantation is high and the cost is greatly reduced. Compared with traditional tissue culture, tissue culture seedlings grow faster and are stronger under sugar-free tissue culture.

[0005] Therefore, the present application provides a novel tissue culture seedling cultivation air circulation device to optimize and regulate the cultivation environment of tissue culture seedlings. Utility Model Content

[0006] The utility model mainly aims at the above problems existing in the prior art and provides a novel tissue culture seedling culture air circulation device.

[0007] The purpose of this utility model is mainly achieved through the following solutions:

[0008] A novel tissue culture seedling cultivation air circulation device comprises a box body, wherein multiple layers of cultivation plates for placing cultivation trays are installed in the box body through two side partitions and a middle partition, and the air circulation device comprises a temperature control module, a humidity control module, a carbon dioxide concentration control module and a controller installed on the box body; the temperature control module comprises a heating box, a refrigeration box, a first pump body, an air intake main pipe and an air intake branch pipe, the outlets of the heating box and the refrigeration box are both connected to the inlet of the first pump body through a second pipeline, the outlet of the first pump body is connected to one end of the air intake main pipe, the other end of the air intake main pipe extends into the box body, the air intake branch pipe is located in the box body and is connected to the side wall of the air intake main pipe; the humidity control module comprises a water storage tank, a water intake main pipe The water tank is installed on the outer wall of the box body, the bottom of the water inlet main pipe extends into the bottom of the water tank, the top of the water inlet main pipe is connected with the inlet of the second pump body, the outlet of the second pump body extends into the box body and is connected with the water outlet main pipe, and a plurality of atomizing nozzles are installed on the water outlet main pipe; the carbon dioxide concentration control module comprises a gas cylinder, an air outlet main pipe and an air outlet, the gas cylinder is installed on the outer wall of the box body through a placement frame, the outlet of the gas cylinder is connected with one end of the air outlet main pipe, the other end of the air outlet main pipe extends into the box body, and the air outlet is installed on the side wall of the air outlet main pipe; a temperature and humidity sensor and a carbon dioxide sensor electrically connected to the controller are also provided in the box body.

[0009] Preferably, the temperature control module also includes an air inlet, a filter box and a first pipe, the air inlet is connected to the inlet of the filter box, the outlet of the filter box is connected to the heating box and the refrigeration box respectively through two first pipes, and the first valve is installed on the first pipe and the second pipe.

[0010] Preferably, the air intake main pipe is arranged vertically, and the air intake branch pipes are arranged in multiple layers, and each layer of the air intake branch pipes is symmetrically provided with two.

[0011] Preferably, a filter element is installed in the filter box, a heating coil is installed in the heating box, and a refrigeration plate is installed in the refrigeration box.

[0012] Preferably, a plurality of exhaust fans are installed on the side walls of the box.

[0013] Preferably, the main water outlet pipe is arranged vertically, and the number of atomizing nozzles corresponds to the number of layers of the cultivation plates.

[0014] Preferably, the gas outlet main pipe is sequentially installed with a second valve and a regulating valve along the flow direction of the carbon dioxide.

[0015] Preferably, the main air outlet pipe is located in a vertical position in the box body, and the air outlets are evenly installed on the side walls of the main air outlet pipe in the box body.

[0016] Therefore, compared with the prior art, the utility model has the following advantages:

[0017] (1) The utility model is provided with a temperature and humidity sensor, a filter box, a heating box, a heating coil, a refrigeration box, a refrigeration sheet, a first pump body, a first valve, an air intake main pipe and an air intake branch pipe. The temperature and humidity sensor can detect the temperature inside the box and transmit the temperature signal to the controller. The controller controls the opening of the heating coil in the heating box or the refrigeration sheet in the refrigeration box, and cooperates with the opening of the first valve and the first pump body to heat or cool the filtered air and blow it into the box through the air intake main pipe and the air intake branch pipe. At the same time, the exhaust fan is controlled to be turned on to discharge the air inside the box, so as to quickly adjust the temperature inside the box.

[0018] (2) The utility model is provided with a temperature and humidity sensor, a water storage tank, a water inlet main pipe, a second pump body, a water outlet main pipe and an atomizing nozzle. The temperature and humidity sensor can detect the humidity in the box and transmit the humidity signal to the controller. The controller controls the second pump body to start, pump out the water in the water storage tank, and spray it out through the atomizing nozzle, thereby quickly adjusting the humidity in the box;

[0019] (3) The utility model is provided with a carbon dioxide sensor, a gas storage bottle, a second valve, a regulating valve, a gas outlet main pipe and a gas outlet. The carbon dioxide sensor can detect the carbon dioxide concentration in the box and transmit the concentration signal to the controller. The controller controls the second valve and the regulating valve to open, and the carbon dioxide in the gas storage bottle is pumped into the box through the gas outlet main pipe and the gas outlet, thereby quickly adjusting the carbon dioxide concentration in the box.

[0020] (4) The utility model adopts sugar-free tissue culture technology to cultivate tissue culture seedlings, artificially controls the temperature, humidity and carbon dioxide concentration in the box, optimizes the growth environment, enables the tissue culture seedlings to take root on their own, improves the photosynthetic capacity and adaptability to the external environment of the virus-free tissue culture seedlings and the rooting quality of the tissue culture seedlings, reduces the microbial contamination rate, promotes the healthy growth of the plants, improves the survival rate of transplanting, and can expand the rapid propagation production of tissue culture seedlings to provide high-quality seedlings, which has important theoretical and practical significance for the healthy development of seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of the utility model;

[0022] Figure 2 This is a schematic diagram of the structure of the utility model without the box door;

[0023] Figure 3 It is a schematic diagram of the internal structure of the utility model.

[0024] Illustrations: 1-box, 2-partitions on both sides, 3-middle partition, 4-culture tray, 5-cultivation plate, 6-controller, 7-heating box, 8-refrigeration box, 9-first pump body, 10-air inlet main pipe, 11-air inlet branch pipe, 12-second pipeline, 13-water storage tank, 14-water inlet main pipe, 15-second pump body, 16-water outlet main pipe, 17-atomizing nozzle, 18-gas storage bottle, 19-air outlet main pipe, 20-air outlet, 21-placing frame, 22-air inlet, 23-filter box, 24-first pipeline, 25-first valve, 26-exhaust fan, 27-second valve, 28-regulating valve, 29-universal wheel, 30-adjustable foot cup, 31-box door, 32-observation window. DETAILED DESCRIPTION

[0025] The technical solution of the utility model is further described in detail below through specific embodiments and in combination with the accompanying drawings. It should be understood that the implementation of the utility model is not limited to the following embodiments, and any form of modification and / or change made to the utility model will fall within the protection scope of the utility model.

[0026] In the present invention, unless otherwise specified, all parts and percentages are weight units, and the equipment and raw materials used can be purchased from the market or are commonly used in the field. The methods in the following embodiments, unless otherwise specified, are conventional methods in the field. The components or equipment in the following embodiments, unless otherwise specified, are universal standard parts or components known to those skilled in the art, and their structures and principles are known to those skilled in the art through technical manuals or conventional experimental methods.

[0027] Embodiment 1:

[0028] like Figure 1 , 2 As shown in Figures 3 and 4, the utility model provides a technical solution, a novel tissue culture seedling culture air circulation device, comprising a box body 1, wherein multiple layers of cultivation boards 5 for placing culture trays 4 are installed in the box body 1 through side partitions 2 and a middle partition 3, four side partitions 2 are vertically arranged, which are respectively fixed to the front and rear ends of the left and right sides of the box body 1 by bolts, and the middle partition 3 is fixed to the middle part of the box body 1 by bolts, and a plurality of through holes are evenly opened on the middle partition 3; a plurality of through holes are evenly opened on the above-mentioned cultivation boards 5, and except for the last layer of cultivation boards 5, the lower surfaces of the other cultivation boards 5 and the top of the box body 1 are installed with fluorescent lamps connected to the controller, and the fluorescent lamps correspond to the culture trays 4 to provide light for the tissue culture seedlings.

[0029] The air circulation device includes a temperature control module, a humidity control module, a carbon dioxide concentration control module and a controller 6 installed on the box 1. The temperature control module is used to control the temperature inside the box 1, the humidity control module is used to control the humidity inside the box 1, and the carbon dioxide concentration control module is used to control the carbon dioxide concentration inside the box 1.

[0030] Specifically, the temperature control module includes a heating box 7, a refrigeration box 8, a first pump body 9, an air intake manifold 10 and an air intake branch pipe 11. The heating box 7, the refrigeration box 8 and the first pump body 9 are all located above the box body 1. The outlets of the heating box 7 and the refrigeration box 8 are connected to the inlet of the first pump body 9 through the second pipe 12. The outlet of the first pump body 9 is connected to one end of the air intake manifold 10, and the other end of the air intake manifold 10 extends into the box body 1. The air intake branch pipe 11 is located in the box body 1 and is connected to the side wall of the air intake manifold 10. A plurality of air holes are evenly arranged on the air intake branch pipe 11.

[0031] Specifically, the humidity control module includes a water tank 13, a water inlet main pipe 14, a second pump body 15, a water outlet main pipe 16, and an atomizing nozzle 17. The water tank 13 and the second pump body 15 are installed in the middle of the left outer wall of the box body 1. The second pump body 15 is located above the water tank 13. The water tank 13 should be provided with a water replenishment port and a drain port. The bottom of the water inlet main pipe 14 extends into the bottom of the water tank 13. The top of the water inlet main pipe 14 is connected with the inlet of the second pump body 15. The outlet of the second pump body 15 extends into the box body 1 and is connected with the water outlet main pipe 16. The water outlet main pipe 16 is equipped with multiple atomizing nozzles 17 facing the cultivation plate 5.

[0032] Specifically, the carbon dioxide concentration control module includes a gas cylinder 18, a gas outlet main pipe 19 and a gas outlet 20. Carbon dioxide is stored in the gas cylinder 18. The gas cylinder 18 is installed on the outer wall of the box body 1 through a placement frame 21. The placement frame 21 is fixedly installed on the right outer wall of the box body 1. The outlet of the gas cylinder 18 is connected to one end of the gas outlet main pipe 19, and the other end of the gas outlet main pipe 19 extends into the box body 1. There are multiple gas outlets 20 and they are all installed on the side wall of the gas outlet main pipe 19.

[0033] Specifically, the box body 1 is also provided with a temperature and humidity sensor and a carbon dioxide sensor electrically connected to the controller 6. The controller 6 adopts a PLC controller and is provided with a touch control screen for setting and displaying parameters. The temperature and humidity sensor and the carbon dioxide sensor are both existing technologies and can be purchased on the market.

[0034] Embodiment 2:

[0035] like Figure 1 , 2As shown in Figures 3 and 4, the utility model provides another technical solution, a novel tissue culture seedling culture air circulation device, which is different from Example 1 in that the temperature control module also includes an air inlet 22, a filter box 23 and a first pipe 24. The filter box 23 is installed above the box body 1, and the air inlet 22 is connected to the inlet of the filter box 23. The outlet of the filter box 23 is respectively connected to the heating box 7 and the refrigeration box 8 through two first pipes 24. The first valve 25 is installed on the first pipe 24 and the second pipe 12. Different first valves 25 are selected to open according to the need to increase or decrease the temperature in the box body 1. When the temperature in the box body 1 needs to be increased, the first valve 25 on the first pipe 24 and the second pipe 12 connected to the heating box 7 is opened. When the temperature in the box body 1 needs to be lowered, the first valve 25 on the first pipe 24 and the second pipe 12 connected to the refrigeration box 8 is opened.

[0036] Specifically, the air intake main pipe 10 is arranged vertically, and the air intake branch pipes 11 are arranged in multiple layers and correspond to the cultivation plates 5 , and two air intake branch pipes 11 are symmetrically arranged on each layer.

[0037] Specifically, a filter element is installed in the filter box 23, which can be purchased on the market and can filter the air entering the box body 1. A heating coil is installed in the heating box 7, and a refrigeration plate is installed in the refrigeration box 8. The heating coil and the refrigeration plate are both electrically connected to the controller.

[0038] Specifically, a plurality of exhaust fans 26 connected to the controller are installed on the left side wall of the box body 1 . The exhaust fans 26 are located at the front and rear of the water storage tank 13 . The exhaust fans 26 can accelerate the air flow in the box body 1 .

[0039] Embodiment 3:

[0040] like Figure 1 , 2 As shown in Figure 3, the utility model provides another technical solution, a novel tissue culture seedling culture air circulation device, which is different from Example 1 in that the water outlet main pipe 16 is vertically arranged, and the number of atomizing nozzles 17 corresponds to the number of layers of the cultivation plate 5. The second pump body 27 is a high-pressure water pump, which can pump out water in the water tank 13 and spray it out through the atomizing nozzle 17, thereby quickly adjusting the humidity in the box body 1.

[0041] Embodiment 4:

[0042] like Figure 1 , 2 As shown in Figures 3 and 4, the utility model provides another technical solution, a novel tissue culture seedling culture air circulation device, which is different from Example 1 in that a second valve 27 and a regulating valve 28 are sequentially installed on the main air outlet pipe 19 along the flow direction of carbon dioxide, and both the second valve 27 and the regulating valve 28 are controlled by a controller.

[0043] Specifically, the main gas outlet pipe 19 is vertically arranged in the box body 1 , and the gas outlets 20 are evenly connected to the side walls of the main gas outlet pipe 19 in the box body 1 .

[0044] The novel tissue culture seedling cultivation air circulation device provided by the utility model can detect the temperature in the box and transmit the temperature signal to the controller through the temperature and humidity sensor, filter box, heating box, heating coil, refrigeration box, refrigeration plate, first pump body, first valve, air intake main pipe and air intake branch pipe, the temperature and humidity sensor can detect the temperature in the box and transmit the temperature signal to the controller, the controller controls to open the heating coil in the heating box or the refrigeration plate in the refrigeration box, and cooperates with the opening of the first valve and the first pump body to heat or cool the filtered air and then blow it into the box through the air intake main pipe and the air intake branch pipe, and at the same time controls the exhaust fan to open to exhaust the air inside the box, so as to quickly adjust the temperature in the box; through the temperature and humidity sensor, water storage tank, water inlet main pipe, second pump body, water outlet main pipe and atomizing nozzle, the temperature and humidity sensor can detect the humidity in the box and transmit the humidity signal to the controller, the controller controls to open the second pump body to extract water in the water storage tank, and spray the water through the atomizing nozzle The carbon dioxide is sprayed out, thereby quickly adjusting the humidity in the box; through the carbon dioxide sensor, gas cylinder, second valve, regulating valve, gas outlet main pipe and gas outlet, the carbon dioxide sensor can detect the carbon dioxide concentration in the box and transmit the concentration signal to the controller, the controller controls the second valve and the regulating valve to open, and the carbon dioxide in the gas cylinder is pumped into the box through the gas outlet main pipe and the gas outlet, thereby quickly adjusting the carbon dioxide concentration in the box; the sugar-free tissue culture technology is used to cultivate tissue culture seedlings, the temperature, humidity and carbon dioxide concentration in the box are artificially controlled, the growth environment is optimized, the tissue culture seedlings can take root by themselves, the self-photosynthetic capacity and adaptability to the external environment of the virus-free tissue culture seedlings and the rooting quality of the tissue culture seedlings are improved, the microbial contamination rate is reduced, the healthy growth of the plants is promoted, the transplant survival rate is improved, and the rapid propagation production of tissue culture seedlings can be expanded to provide high-quality seedlings, which has great theoretical and practical significance for the healthy development of seedlings.

[0045] It should be understood that this embodiment is only used to illustrate the present invention and is not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

Claims

1. A novel tissue culture seedling cultivation air circulation device, comprising a box (1), wherein multiple layers of cultivation plates (5) for placing cultivation trays (4) are installed in the box (1) through two side partitions (2) and a middle partition (3), and characterized in that: The air circulation device comprises a temperature control module, a humidity control module, a carbon dioxide concentration control module and a controller (6) which are installed on a box body (1); The temperature control module comprises a heating box (7), a refrigeration box (8), a first pump body (9), an air intake main pipe (10) and an air intake branch pipe (11); the outlets of the heating box (7) and the refrigeration box (8) are both connected to the inlet of the first pump body (9) through a second pipe (12); the outlet of the first pump body (9) is connected to one end of the air intake main pipe (10); the other end of the air intake main pipe (10) extends into the box body (1); and the air intake branch pipe (11) is located in the box body (1) and is connected to the side wall of the air intake main pipe (10); The humidity control module comprises a water storage tank (13), a water inlet main pipe (14), a second pump body (15), a water outlet main pipe (16), and an atomizing nozzle (17); the water storage tank (13) is mounted on the outer wall of the box body (1); the bottom of the water inlet main pipe (14) extends into the bottom of the water storage tank (13); the top of the water inlet main pipe (14) is connected to the inlet of the second pump body (15); the outlet of the second pump body (15) extends into the box body (1) and is connected to the water outlet main pipe (16); and a plurality of atomizing nozzles (17) are mounted on the water outlet main pipe (16); The carbon dioxide concentration control module comprises a gas storage bottle (18), a gas outlet main pipe (19) and a gas outlet (20); the gas storage bottle (18) is mounted on the outer side wall of the box body (1) via a placement frame (21); the outlet of the gas storage bottle (18) is connected to one end of the gas outlet main pipe (19); the other end of the gas outlet main pipe (19) extends into the box body (1); and the gas outlet (20) is mounted on the side wall of the gas outlet main pipe (19); The box body (1) is also provided with a temperature and humidity sensor and a carbon dioxide sensor which are electrically connected to the controller (6).

2. The novel tissue culture seedling cultivation air circulation device according to claim 1 is characterized in that: The temperature control module further comprises an air inlet (22), a filter box (23) and a first pipe (24); the air inlet (22) is connected to the inlet of the filter box (23); the outlet of the filter box (23) is connected to the heating box (7) and the refrigeration box (8) respectively through two first pipes (24); and the first valve (25) is installed on both the first pipe (24) and the second pipe (12).

3. The novel tissue culture seedling cultivation air circulation device according to claim 2 is characterized in that: The air intake main pipe (10) is arranged vertically, and the air intake branch pipes (11) are arranged in multiple layers, and each layer of the air intake branch pipes (11) is provided with two symmetrical air intake branch pipes (11) on the left and right.

4. The novel tissue culture seedling cultivation air circulation device according to claim 3 is characterized in that: A filter core is installed in the filter box (23), a heating coil is installed in the heating box (7), and a refrigeration plate is installed in the refrigeration box (8).

5. The novel tissue culture seedling cultivation air circulation device according to claim 4 is characterized in that: A plurality of exhaust fans (26) are installed on the side walls of the box body (1).

6. The novel tissue culture seedling cultivation air circulation device according to claim 1 is characterized in that: The water outlet main pipe (16) is arranged vertically, and the number of atomizing nozzles (17) corresponds to the number of layers of the cultivation plates (5).

7. The novel tissue culture seedling cultivation air circulation device according to claim 1 is characterized in that: The gas outlet main pipe (19) is provided with a second valve (27) and a regulating valve (28) in sequence along the flow direction of the carbon dioxide.

8. The novel tissue culture seedling cultivation air circulation device according to claim 7 is characterized in that: The main air outlet pipe (19) is arranged vertically in the box body (1), and the air outlets (20) are evenly installed on the side walls of the main air outlet pipe (19) in the box body (1).

Images