Multi-position preparative column valve switching system for polysaccharide gel purification system

Abstract

The utility model provides a multi-position preparative column valve switching system for a polysaccharide gel purification system, which comprises a sample introduction unit, a solvent elution unit, a multi-position pump, a multi-position switching chromatographic column, a detector and a collector, the sample introduction unit and the solvent elution unit are respectively connected with the liquid inlet end of the multi-position switching chromatographic column through the multi-position pump; and the liquid outlet end of the multi-position switching chromatographic column is connected with the detector. According to the technical scheme, automation of the whole process of online liquid preparation, online elution, online detection and online collection is achieved through an upper computer system customized by a company, unmanned operation of an instrument can be achieved, the stability of the whole process is enhanced, then the stability of a polysaccharide component detection result is improved, and the efficiency is greatly improved; switching of different solvents and different eluents can be achieved, meanwhile, chromatographic columns of different specifications can be selected according to actual use requirements, rapid switching is carried out, the time cost is saved, meanwhile, the chromatographic columns do not need to be frequently replaced, and efficiency is greatly improved.

Description

Technical Field

[0001] The utility model relates to a switching system, in particular to a multi-position preparation column valve switching system for a polysaccharide gel purification system. Background Art

[0002] Polysaccharides are a kind of biological macromolecules. According to the different charges of polysaccharides, they can be divided into neutral polysaccharides, acidic polysaccharides and alkaline polysaccharides. Conventional separation uses ion exchange fillers for separation. Sodium chloride elution solutions with different gradient concentrations are manually configured for elution. The components received are collected tube by tube and tested using the phenol-sulfuric acid method or the anthrone-sulfuric acid method. At the same time, it is impossible to quickly switch between different solvents and different eluents, and it is impossible to select chromatographic columns of different specifications according to actual use needs. In this process, it is almost entirely operated manually, which wastes the precious time of researchers and also has a certain impact on the stability of the results. Based on the above problems, our company has developed a multi-position preparation column valve switching system for polysaccharide gel purification system. Utility Model Content

[0003] The utility model aims to provide a multi-position preparation column valve switching system for a polysaccharide gel purification system to solve the problems raised in the above-mentioned background technology.

[0004] In order to solve the above technical problems, the utility model provides the following technical solutions: a multi-position preparation column valve switching system for a polysaccharide gel purification system, comprising an injection unit, a solvent elution unit, a multi-position pump, a multi-position switching chromatographic column, a detector and a collector, wherein the injection unit and the solvent elution unit are respectively connected to the liquid inlet end of the multi-position switching chromatographic column through the multi-position pump, and the liquid outlet end of the multi-position switching chromatographic column is connected to the detector.

[0005] The solvent elution unit comprises a solvent unit and an elution unit.

[0006] A switching pump A is provided in the solvent unit, and a switching pump B is provided in the elution unit.

[0007] The switching channel of the switching pump A is 2-12 positions, and the switching channel of the switching pump B is 2-4 positions.

[0008] The multi-position switching chromatographic column comprises a multi-position switching pump and a plurality of chromatographic columns.

[0009] The number of the chromatographic columns is 4 to 8.

[0010] Also includes collector.

[0011] The collector and the detector are connected in series via an infusion pump.

[0012] The collector and the detector are connected in parallel via an infusion pump

[0013] Compared with the prior art, the beneficial effects achieved by the utility model are:

[0014] 1. The technical solution in this application realizes the automation of the whole process of online liquid preparation, online elution, online detection and online collection through the customized upper computer system of our company. The instrument can realize unmanned operation and can realize 24-hour online operation, which improves the work efficiency of scientific researchers. The automated control enhances the stability of the whole process, thereby improving the stability of the polysaccharide component detection results and greatly improving the efficiency.

[0015] 2. The present application adopts a multi-channel switching pump A and a switching pump B in conjunction with a multi-position switching chromatographic column setting, which can realize the switching of different solvents and different eluents, thereby improving the applicability of the entire system. At the same time, chromatographic columns of different specifications can be selected according to actual use needs for rapid switching. Switching can be achieved through the system's time program setting, which saves time costs and does not require frequent replacement of chromatographic columns, greatly improving efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. In the accompanying drawings:

[0017] Figure 1 This is a schematic diagram of the structure of a multi-position preparation column valve switching system for a polysaccharide gel purification system of the utility model. Figure 1 ;

[0018] Figure 2 This is a schematic diagram of the structure of a multi-position preparation column valve switching system for a polysaccharide gel purification system of the utility model. Figure 2 ;

[0019] In the figure: 1 injection unit; 2 solvent elution unit; 21 solvent unit; 22 elution unit; 3 multi-position pump; 4 multi-position switching chromatographic column; 41 multi-position switching pump; 42 multiple chromatographic columns; 5 detector; 6 collector; 7 switching pump A; 8 switching pump B. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] Example 1

[0022] See also Figure 1 or Figure 2 The utility model provides a technical solution: a multi-position preparation column valve switching system for a polysaccharide gel purification system, comprising an injection unit 1, a solvent elution unit 2, a multi-position pump 3, a multi-position switching chromatographic column 4, a detector 5 and a collector 6, wherein the solvent elution unit 2 comprises a solvent unit 21 and an elution unit 22, a switching pump A7 is arranged in the solvent unit 21, and a switching pump B8 is arranged in the elution unit 22, the switching channel of the switching pump A7 is 8 positions, and the switching channel of the switching pump B8 is 2 positions, the injection unit 1, the solvent unit 21 and the elution unit 22 are respectively connected to the liquid inlet end of the multi-position switching chromatographic column 4 through the multi-position pump 3, and the liquid outlet end of the multi-position switching chromatographic column 4 is connected to the detector 5, the multi-position switching chromatographic column 4 comprises a multi-position switching pump 41 and a plurality of chromatographic columns 42, the switching channel of the multi-position switching pump is adapted to the number of chromatographic columns 42, there are 4 chromatographic columns 42, and 4 chromatographic columns 42 of different specifications can be selected for rapid switching.

[0023] The specific working principle of Example 1: The quantitative polysaccharide sample and the solvent are respectively output and mixed by the sampling unit 1 through the infusion pump and the solvent unit 21 through the switching pump A7, and then transported to a chromatographic column 42 in the multi-position switching chromatographic column 4 through the multi-position pump 3 for separation. The various components after separation are transported to the detector 5 for detection through the infusion pump, and the remaining amount after detection enters the collector 6 for collection. The switching channel of the switching pump A7 is 8 positions, which can quickly switch 8 different solvents, such as deionized water, sodium chloride solution, sodium acetate solution, sodium hydroxide solution or acetic acid solution, etc.;

[0024] It also includes a collector 6, which is connected in series with the detector 5 through an infusion pump, or the collector 6 and the detector 5 are connected in parallel through an infusion pump. After the detection of the various different components separated from the polysaccharide is completed, the eluent in the elution unit 22 is transported by the switching pump B8, and is input into the chromatographic column 42 by the multi-position pump 3 to clean the internal impurities, and the cleaning liquid enters the collector 6 after being discharged. The switching channel of the switching pump B8 is 2 positions, which can switch between 2 different solutions.

[0025] Example 2

[0026] The switching channel of the switching pump A7 is 10 positions, which can quickly switch 10 different solvents, the switching channel of the switching pump B8 is 3 positions, which can switch 3 different solutions, and there are 6 chromatographic columns 42, which can select 6 chromatographic columns 42 of different specifications for rapid switching. The rest of the technical scheme is the same as that of Example 1.

[0027] Example 3

[0028] The switching channel of the switching pump A7 is 12 positions, which can quickly switch 12 different solvents, the switching channel of the switching pump B8 is 4 positions, which can switch 4 different solutions, and there are 8 chromatographic columns 42, which can select 8 chromatographic columns 42 of different specifications for rapid switching. The rest of the technical scheme is the same as that of Example 1.

[0029] It should be noted that relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises", or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article, or device.

[0030] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A multi-position preparative column valve switching system for a polysaccharide gel purification system, characterized in that: The invention comprises a sample injection unit (1), a solvent elution unit (2), a multi-position pump (3), a multi-position switching chromatographic column (4) and a detector (5); the sample injection unit (1) and the solvent elution unit (2) are respectively connected to the liquid inlet end of the multi-position switching chromatographic column (4) through the multi-position pump (3), and the liquid outlet end of the multi-position switching chromatographic column (4) is connected to the detector (5).

2. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 1, characterized in that: The solvent elution unit (2) comprises a solvent unit (21) and an elution unit (22).

3. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 2, characterized in that: A switching pump A (7) is provided in the solvent unit (21), and a switching pump B (8) is provided in the elution unit (22).

4. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 3, characterized in that: The switching channel of the switching pump A (7) is 2-12 positions, and the switching channel of the switching pump B (8) is 2-4 positions.

5. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 1, characterized in that: The multi-position switching chromatographic column (4) comprises a multi-position switching pump (41) and a plurality of chromatographic columns (42).

6. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 5, characterized in that: The number of the chromatographic columns (42) is 4 to 8.

7. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 1, characterized in that: A collector (6) is also included.

8. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 7, characterized in that: The collector (6) and the detector (5) are connected in series via an infusion pump.

9. The multi-position preparative column valve switching system for a polysaccharide gel purification system according to claim 7, characterized in that: The collector (6) and the detector (5) are connected in parallel via an infusion pump.

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