Centrifugal-free EP tube

Abstract

The centrifugation-free EP tube comprises a tube body, a plug cover and a base, an opening is formed in the upper end of the tube body, a liquid inlet and a liquid outlet are formed in the bottom of the tube body, and the position of the liquid inlet is lower than that of the liquid outlet; the plug cover is detachably plugged on the opening of the tube body; the base is integrally arranged at the lower end of the pipe body; a first micro-channel, a second micro-channel and a reaction cavity are arranged in the base, the bottom of the reaction cavity is communicated with the liquid inlet through the first micro-channel, and the top of the reaction cavity is communicated with the liquid outlet through the second micro-channel. The centrifugation-free EP tube provided by the utility model can effectively eliminate bubbles in liquid, does not need centrifugation or other operations, and is convenient to use.

Description

Technical Field

[0001] The utility model relates to the technical field of biochemical detection instruments, in particular to a centrifugation-free EP tube. Background Art

[0002] EP tubes, also known as microcentrifuge tubes, are commonly used equipment in medical or biological laboratories and inspection rooms. EP tubes are usually made of polypropylene, which is transparent and light. EP tubes are basically made of one-piece injection molding and do not have too much structural design, so the product cost is very low.

[0003] When conducting biochemical experiments, bubbles will inevitably be generated after adding liquid to the EP tube, and the generation of bubbles often has an important impact on the experimental results, especially for some experiments with high precision requirements such as Real-Time PCR, CCK-8, etc. In order to avoid the influence of bubbles on the experiment, researchers often use the following methods to remove bubbles: 1. Use a pipette to suck or poke bubbles. When using a pipette to suck out bubbles, it is inevitable that part of the liquid will be sucked out while sucking out the bubbles, resulting in inaccurate liquid volume and affecting the experimental results. However, using a pipette to poke bubbles often requires high operation requirements, low efficiency, and it is difficult to get satisfactory results. 2. Centrifuge: The centrifuge method can eliminate some larger bubbles, while smaller bubbles are often difficult to eliminate, so repeated centrifugation is required. Its efficiency is low and the results are difficult to guarantee. 3. Use fingers to flick: Using fingers to flick often removes old bubbles and produces new bubbles.

[0004] Therefore, it is necessary to provide an EP tube that can effectively eliminate bubbles. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide a centrifugation-free EP tube, which can effectively eliminate bubbles in liquid without centrifugation or other operations.

[0006] In order to solve the above technical problems, the utility model provides the following technical solutions:

[0007] The utility model provides a centrifugation-free EP tube, comprising:

[0008] The tube body has an opening at the upper end and a liquid inlet and a liquid outlet at the bottom, and the position of the liquid inlet is lower than the position of the liquid outlet;

[0009] A plug cover, detachably sealed on the opening of the tube body; and

[0010] A base is integrally arranged at the lower end of the tube body; a first microchannel, a second microchannel and a reaction chamber are arranged in the base, the bottom of the reaction chamber is connected to the liquid inlet through the first microchannel, and the top of the reaction chamber is connected to the liquid outlet through the second microchannel.

[0011] Furthermore, the reaction chamber is thick in the middle and thin at the upper and lower ends.

[0012] Furthermore, the reaction chamber has a shape of square, diamond, circle, ellipse, or spindle.

[0013] Furthermore, the plug cover is connected to the tube body via a flexible connecting strip.

[0014] Furthermore, the plug cover comprises:

[0015] A cover body, one end of which is connected to the flexible connecting strip; and

[0016] A plug is integrally formed on the lower side of the cover; a truncated cone with an open bottom is formed at the lower end of the plug;

[0017] When the plug is inserted into the tube body, the truncated cone portion abuts against the interior of the tube body to achieve sealing.

[0018] Further, a guide disc is formed in the middle of the plug, and the diameter of the guide disc is smaller than the diameter of the tube body; and / or,

[0019] A sealing disc is formed at the upper end of the plug, and the diameter of the sealing disc is equal to the diameter of the opening of the tube.

[0020] Furthermore, the bottom wall of the tube body is a sloped surface, and the liquid inlet and the liquid outlet are both located on the sloped surface.

[0021] Furthermore, the base is a vertical plate-shaped base, and its lower end is pointed.

[0022] Furthermore, the base is provided with a plurality of weight-reducing grooves; and / or,

[0023] The base is also provided with at least one auxiliary positioning hole.

[0024] Furthermore, a reinforcement frame is formed on the base, the outer peripheral surface of the tube body is fixedly connected to the reinforcement frame, and one end of the flexible connecting strip is connected to the reinforcement frame.

[0025] Compared with the prior art, the beneficial effects of the utility model are:

[0026] The centrifugation-free EP tube provided by the utility model has a base at the lower end of the tube body of the ordinary EP tube, and the first microchannel, the second microchannel and the reaction chamber are further arranged in the base, and a liquid inlet and a liquid outlet are arranged at the bottom of the tube body; wherein the bottom of the reaction chamber is connected to the liquid inlet through the first microchannel, and the top of the reaction chamber is connected to the liquid outlet through the second microchannel. Therefore, when liquid is added to the tube body of the EP tube, bubbles will be generated in the liquid. Since the position of the liquid inlet is lower than the position of the liquid outlet, the added liquid will enter the first microchannel through the liquid inlet, and then enter the reaction chamber from the bottom through the first microchannel, and gather in the reaction chamber. In this process, the bubbles in the liquid in the reaction chamber will gradually float to the liquid surface. As the liquid level in the reaction chamber rises, the bubbles are continuously squeezed, and finally enter the second microchannel from the top of the reaction chamber, and finally discharged from the liquid outlet. In this way, in the process of adding liquid to the EP tube, the elimination of bubbles in the liquid is realized, without the need for other operations such as centrifugation, which is very convenient and has a good effect of eliminating bubbles. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic structural diagram of a centrifugation-free EP tube in a closed state according to the utility model;

[0028] Figure 2 for Figure 1 A schematic diagram of the structure of the centrifugation-free EP tube in the open state;

[0029] Explanation of reference numerals in the figure: 100, pipe body; 110, pipe opening; 120, slope surface; 130, liquid inlet; 140, liquid outlet; 150, reinforcement frame;

[0030] 200, plug cover; 210, cover body; 220, plug column; 221, sealing disc body; 222, guide disc body; 223, truncated cone portion; 230, flexible connecting strip;

[0031] 300, base; 310, first microchannel; 320, second microchannel; 330, reaction chamber; 340, weight reduction groove; 350, auxiliary positioning hole. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0033] In the drawings, components with the same structure are indicated by the same numerical labels, and components with similar structures or functions are indicated by similar numerical labels. Directional terms mentioned in the present invention, such as up, down, front, back, left, right, inside, outside, upper surface, lower surface, side, top surface, bottom, front end, rear end, end, etc., are only directions in the drawings and are only used to explain and illustrate the present invention, but not to limit the scope of protection of the present invention.

[0034] In the drawings, components with the same structure are indicated by the same numerical reference numerals. When some components are described as being "on" another component, the component may be directly placed on the other component; there may also be an intermediate component on which the component is placed, and the intermediate component is placed on the other component. When a component is described as being "mounted to" or "connected to" another component, the two may be understood to be directly "mounted" or "connected", or one component may be indirectly "mounted to" or "connected to" another component through an intermediate component.

[0035] As described in the background technology, when performing biochemical experiments, bubbles will inevitably be generated after adding liquid into the EP tube, and the generation of bubbles often has an important impact on the experimental results. In order to avoid the influence of bubbles on the experiment, researchers have adopted various means to eliminate bubbles, such as using a pipette to suck out or prick bubbles, centrifugal removal of bubbles, finger flicking to remove bubbles, etc., but these methods all have various disadvantages, either the effect of removing bubbles is not good, or the operation requirements are high and difficult to meet the requirements.

[0036] In view of this technical problem, the utility model provides a centrifugation-free EP tube, which can effectively eliminate bubbles in the liquid by itself without centrifugation or other operations.

[0037] See also Figure 1-2 The centrifugation-free EP tube of one embodiment of the utility model comprises a tube body 100, a plug cover 200 and a base 300. The tube body 100 has a tube opening 110 at its upper end, and the plug cover 200 is detachably sealed on the tube opening 110. The bottom of the tube body 100 is provided with a liquid inlet 130 and a liquid outlet 140, and the position of the liquid inlet 130 is lower than the position of the liquid outlet 140. Here, "lower than" means that when the EP tube is in an upright state, the level of the liquid inlet 130 is lower than the level of the liquid outlet 140.

[0038] The base 300 is integrally arranged at the lower end of the tube body 100, and a first microchannel, a second microchannel and a reaction chamber 330 are arranged in the base 300. The bottom of the reaction chamber 330 is connected to the liquid inlet 130 through the first microchannel, and the top of the reaction chamber 330 is connected to the liquid outlet 140 through the second microchannel.

[0039] The centrifugation-free EP tube provided by the utility model has a base 300 arranged at the lower end of the tube body 100 of the ordinary EP tube, and the first microchannel, the second microchannel and the reaction chamber 330 are further arranged in the base 300, and the liquid inlet 130 and the liquid outlet 140 are arranged at the bottom of the tube body 100; wherein the bottom of the reaction chamber 330 is connected with the liquid inlet 130 through the first microchannel, and the top of the reaction chamber 330 is connected with the liquid outlet 140 through the second microchannel. Therefore, when liquid is added to the tube body 100 of the EP tube, bubbles will be generated in the liquid. Since the position of the liquid inlet 130 is lower than the position of the liquid outlet 140, the added liquid will enter the first microchannel through the liquid inlet 130, and then enter the reaction chamber 330 from the bottom through the first microchannel, and gather in the reaction chamber 330. In this process, the bubbles in the liquid in the reaction chamber 330 will gradually float to the liquid surface. As the liquid level in the reaction chamber 330 rises, the bubbles are continuously squeezed and eventually enter the second microchannel 320 from the top of the reaction chamber 330 and are finally discharged from the liquid outlet 140. In this way, in the process of adding liquid to the EP tube, the bubbles in the liquid are eliminated without the need for other operations such as centrifugation, which is very convenient and has a good effect of eliminating bubbles.

[0040] In the present invention, the reaction chamber 330 is preferably thick in the middle and thin at the upper and lower ends. The lower end of the reaction chamber 330 is thinner, so that when the liquid is introduced from the bottom of the reaction chamber 330, the liquid level can rise quickly, so that the bubbles in the liquid can float to the liquid surface in time; similarly, the upper end of the reaction chamber 330 is thinner, and as the liquid level rises, the bubbles on the liquid surface will be guided into the second microchannel 320 and discharged, avoiding re-entry into the liquid, thereby improving the effect of removing bubbles. In some embodiments, the shape of the reaction chamber 330 can be one of square, rhombus, round, elliptical, and spindle, preferably square or rhombus.

[0041] In the present invention, the diameters of the first microchannel 310 and the second microchannel 320 are between 10 and 1000 micrometers, for example, 10 micrometers, 50 micrometers, 100 micrometers, 200 micrometers, 400 micrometers, 500 micrometers, 800 micrometers, 1000 micrometers, etc.

[0042] In the present invention, the plug cover 200 covers the opening of the tube body 100, thereby achieving the sealing of the tube body 100. Preferably, the plug cover 200 is connected to the tube body 100 through a flexible connecting strip 230.

[0043] In some embodiments, the plug cover 200 includes a cover body 210 and a plug 220, wherein one end of the cover body 210 is connected to a flexible connecting strip 230. The plug 220 is integrally formed on the lower side of the cover body 210, and a truncated cone portion 223 with a bottom opening is formed at the lower end of the plug 220. When the plug 220 is inserted into the tube body 100, the truncated cone portion 223 abuts against the inside of the tube body 100 to achieve sealing. The truncated cone portion 223 is thick at the lower end and gradually becomes thinner upwards, so that it is easy to pass through the tube mouth 110 obliquely and be inserted into the tube body 100. In the process of inserting the plug 220 downward, the lower end of the truncated cone portion 223 gradually contacts and abuts against the inner wall surface of the tube body 100, thereby achieving sealing.

[0044] In some embodiments of the present invention, a guide disc 222 is formed in the middle of the plug 220. The guide disc 222 is circular and is integrally formed with the plug 220. The diameter of the guide disc 222 is slightly smaller than the diameter of the tube 100. When the plug 220 is inserted downward, the guide disc 222 can play a guiding role.

[0045] In some embodiments of the present invention, a sealing disc 221 is formed at the upper end of the plug 220, and the sealing disc 221 is also circular and is integrally formed with the plug 220. The diameter of the sealing disc 221 is equal to the diameter of the opening of the tube 100, so that when the plug 220 is inserted into place, the sealing disc 221 can seal the opening of the tube 100.

[0046] In some embodiments of the present invention, the bottom wall of the tube body 100 is a slope surface 120, and the liquid inlet 130 and the liquid outlet 140 are both located on the slope surface 120. The liquid inlet 130 is located at the lower side of the slope surface 120, and the liquid outlet 140 is located at the upper end of the slope surface 120. In this embodiment, the bottom wall of the tube body 100 is a slope surface 120, so that the added liquid can quickly flow to the bottom of the slope along the slope surface 120, and then flow into the first microchannel 310 through the liquid inlet 130.

[0047] In some embodiments of the present invention, the base 300 is a vertical plate-shaped base 300. The vertical plate-shaped base 300 can meet the space requirements for arranging the first microchannel 310, the second microchannel 320 and the reaction chamber 330 therein, and can save materials, reduce the weight of the EP tube, and reduce costs. The lower end of the base 300 is preferably pointed, so as to facilitate the placement of the EP tube in the tube seat.

[0048] In some embodiments of the present invention, a plurality of weight-reducing grooves 340 are provided on both sides of the base 300. In other embodiments, at least one auxiliary positioning hole 350 is provided on the base 300 for positioning the EP tube.

[0049] In some embodiments of the present invention, a reinforcement frame 150 is also formed on the base 300. Figure 1 The reinforcement frame 150 is disposed above the base 300 and is integrally formed with the base 300 . The outer peripheral surface of the tube body 100 is fixedly connected to the reinforcement frame 150 , and one end of the flexible connection strip 230 is connected to the reinforcement frame 150 .

[0050] In summary, the EP tube provided by the utility model can effectively discharge bubbles in the liquid during the liquid adding process without centrifugation or other operations, is easy to use, and has a good effect of eliminating bubbles.

[0051] The above-described embodiments are only preferred embodiments for fully illustrating the present utility model, and the protection scope of the present utility model is not limited thereto. Equivalent substitutions or changes made by technicians in the technical field on the basis of the present utility model are all within the protection scope of the present utility model. The protection scope of the present utility model shall be subject to the claims.

Claims

1. A centrifuge-free EP tube, characterized in that: include: The tube body has an opening at the upper end and a liquid inlet and a liquid outlet at the bottom, and the position of the liquid inlet is lower than the position of the liquid outlet; A plug cover, detachably sealed on the opening of the tube body; and A base is integrally arranged at the lower end of the tube body; a first microchannel, a second microchannel and a reaction chamber are arranged in the base, the bottom of the reaction chamber is connected to the liquid inlet through the first microchannel, and the top of the reaction chamber is connected to the liquid outlet through the second microchannel.

2. The centrifuge-free EP tube according to claim 1, characterized in that: The reaction chamber is thick in the middle and thin at the upper and lower ends.

3. The centrifugation-free EP tube according to claim 2, characterized in that: The reaction chamber has a shape of square, rhombus, circle, ellipse or spindle.

4. The centrifugation-free EP tube according to claim 1, characterized in that: The plug cover is connected to the tube body through a flexible connecting strip.

5. The centrifuge-free EP tube according to claim 4, characterized in that: The plug cover comprises: A cover body, one end of which is connected to the flexible connecting strip; and A plug is integrally formed on the lower side of the cover; a truncated cone with an open bottom is formed at the lower end of the plug; When the plug is inserted into the tube body, the truncated cone portion abuts against the interior of the tube body to achieve sealing.

6. The centrifuge-free EP tube according to claim 5, characterized in that: A guide disc is formed in the middle of the plug, and the diameter of the guide disc is smaller than the diameter of the tube; and / or, A sealing disc is formed at the upper end of the plug, and the diameter of the sealing disc is equal to the diameter of the opening of the tube.

7. The centrifuge-free EP tube according to claim 1, characterized in that: The bottom wall of the tube body is a slope surface, and the liquid inlet and the liquid outlet are both located on the slope surface.

8. The centrifugation-free EP tube according to claim 1, characterized in that: The base is a vertical plate-shaped base, and the lower end thereof is in a pointed shape.

9. The centrifuge-free EP tube according to claim 1, characterized in that: The base is provided with a plurality of weight-reducing grooves; and / or, The base is also provided with at least one auxiliary positioning hole.

10. The centrifuge-free EP tube according to claim 4, characterized in that: A reinforcement frame is also formed on the base, the outer peripheral surface of the tube body is fixedly connected to the reinforcement frame, and one end of the flexible connecting strip is connected to the reinforcement frame.

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