High-applicability exosome extraction device

Abstract

The utility model belongs to the technical field of exosome extraction devices, and particularly relates to a high-applicability exosome extraction device which comprises a bottom shell, a rotating disc is rotatably connected to the inner circular wall of the bottom shell through a rotating groove, a set of first C-shaped pipes are fixedly connected to the top of the rotating disc, a set of sliding grooves are formed in the top of the rotating disc, and the first C-shaped pipes are fixedly connected to the bottom of the bottom shell. A set of sliding grooves are formed in the bottom shell, fixing blocks are slidably connected into the set of sliding grooves through sliding blocks, second C-shaped pipes are fixedly connected to one sides of the fixing blocks, a moving part is arranged on the rotating disc and used for driving the set of second C-shaped pipes to move, and a power part is arranged at the bottom of the bottom shell. The problems that a centrifuge tube is placed in an insertion hole, but the insertion hole is generally of a fixed size and can only be suitable for a centrifuge tube of a single size, so that the experiment requirement is limited, the use is not convenient enough, a worker needs to replace a proper centrifuge, and the exosome extraction efficiency is influenced are solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of exosome extraction devices, and specifically relates to an exosome extraction device with high applicability. Background Art

[0002] Exosomes are nanospheres with a diameter between 40 and 100 nm and a double-layer membrane structure, mainly composed of lipids, proteins and genetic material. There are many methods for extracting exosomes, the most commonly used of which are kit extraction and ultracentrifugation. Ultracentrifugation is a commonly used method for extracting exosomes. Usually, a test tube containing the extract is placed on a centrifuge, and the centrifuge drives the test tube to rotate, so as to achieve the purpose of centrifugal extraction of exosomes.

[0003] In the prior art, a centrifuge is generally provided with several sockets for placing centrifuge tubes in the sockets. However, the sockets are generally of fixed size and can only accommodate centrifuge tubes of a single size, which limits the requirements of the experiment and is not convenient to use. The staff needs to replace the centrifuge with a suitable one, which affects the efficiency of exosome extraction.

[0004] To this end, the utility model provides an exosome extraction device with high applicability. Utility Model Content

[0005] In order to make up for the shortcomings of the existing technology, a general centrifuge is provided with several sockets for placing centrifuge tubes in the sockets, but the sockets are generally of fixed size and can only be used for centrifuge tubes of a single size, which limits the requirements of the experiment and is not convenient to use. The staff needs to replace the appropriate centrifuge, which affects the efficiency of exosome extraction.

[0006] The technical solution adopted by the utility model to solve its technical problems is: the utility model describes an exosome extraction device with high applicability, comprising a bottom shell, a rotating disk is rotatably connected to the inner circular wall of the bottom shell through a rotating groove, a group of first C-shaped tubes are fixedly connected to the top of the rotating disk, a group of sliding grooves are opened on the top of the rotating disk, a group of sliding grooves are slidably connected to a fixed block through a sliding block, a second C-shaped tube is fixedly connected to one side of the fixed block, a moving part is provided on the rotating disk, the moving part is used to drive a group of second C-shaped tubes to move, and a power part is provided at the bottom of the bottom shell, the power part is used to drive the rotating disk to rotate.

[0007] Preferably, the movement includes a first through slot, a group of first through slots are respectively opened on a group of fixed blocks, the rotating disk is fixedly connected to an electric telescopic rod through a fixed through hole, the telescopic end of the electric telescopic rod is fixedly connected to a circular block, a group of second through slots are opened on the edge of the circular block, a group of first through slots are rotatably connected with connecting rods between opposite side walls through a first rotating shaft, and the other ends of a group of connecting rods are rotatably connected between opposite side walls of a group of second through slots through a second rotating shaft.

[0008] Preferably, the power component includes a motor, which is fixed to the inner circular wall of the bottom shell through a circular rod, and a driving rod is provided at the output end of the motor. A first gear is fixed to the bottom of the driving rod, and the first gear is rotatably connected to the inner bottom wall of the bottom shell through a third rotating shaft. A second gear is fixed to the bottom of the electric telescopic rod, and the second gear is rotatably connected to the inner bottom wall of the bottom shell through a fourth rotating shaft, and the first gear and the second gear are meshed with each other.

[0009] Preferably, a group of square rods are slidably connected to the first C-shaped tube via a group of square through holes, one end of the group of square rods is fixedly connected to an extrusion plate, and the extrusion plate is located on the inner side of the first C-shaped tube, the other end of the group of square rods is fixedly connected to a square block, the square block is threadedly connected to a threaded rod via a threaded hole, one end of the threaded rod is rotatably connected to the outer circular wall of the first C-shaped tube, and the other end of the threaded rod is fixedly connected to a rotating valve.

[0010] Preferably, a first fixing rod is fixedly connected to the top of the circular block, and a top cover is fixedly connected to the top of the first fixing rod.

[0011] Preferably, rubber pads are provided on the inner sides of the first C-shaped tube, the second C-shaped tube and the extrusion plate.

[0012] The beneficial effects of the utility model are as follows:

[0013] 1. The highly applicable exosome extraction device described in the utility model drives a group of second C-shaped tubes to move through a moving part, and can adjust the distance between the first C-shaped tube and the second C-shaped tube, so as to fix centrifuge tubes of different sizes. The power part drives the rotating disk to rotate and perform centrifugal operation, thereby avoiding the prior art that can only be used for centrifuge tubes of a single size, which limits the requirements of the experiment, is not convenient to use, and requires the staff to replace the appropriate centrifuge, which affects the efficiency of exosome extraction.

[0014] 2. The highly applicable exosome extraction device described in the utility model, through the provided threaded rod and extrusion plate, can enable the staff to rotate the rotary valve to squeeze the centrifuge tube with a smaller diameter through the extrusion plate when it is necessary to perform centrifugal operation on the centrifuge tube with a smaller diameter, so as to achieve a fixing effect, so that the extraction device can adapt to centrifuge tubes of various sizes, and can avoid the problem that when the centrifuge tube has a small diameter and is not sufficiently matched, the centrifuge tube may be subjected to excessive force and rupture during high-speed rotation. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model will be further described below in conjunction with the accompanying drawings.

[0016] Figure 1 It is a three-dimensional diagram of the utility model;

[0017] Figure 2 It is a schematic diagram of a circular block in the utility model;

[0018] Figure 3 It is a cross-sectional view of the bottom shell in the utility model;

[0019] Figure 4 It is a schematic diagram of the first fixing rod in the utility model;

[0020] Figure 5 It is a cross-sectional view of the threaded rod in the utility model;

[0021] In the figure: 1, bottom shell; 2, rotating disk; 3, first C-shaped tube; 4, sliding groove; 5, fixed block; 6, second C-shaped tube; 7, first through groove; 8, electric telescopic rod; 9, round block; 10, second through groove; 11, connecting rod; 12, motor; 13, driving rod; 14, first gear; 15, second gear; 16, square rod; 17, extrusion plate; 18, square block; 19, threaded rod; 20, rotating valve; 21, first fixed rod; 22, top cover. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] like Figures 1 to 5As shown, a highly applicable exosome extraction device described in an embodiment of the utility model comprises a bottom shell 1, a rotating disk 2 is rotatably connected to the inner circular wall of the bottom shell 1 through a rotating groove, a group of first C-shaped tubes 3 are fixedly connected to the top of the rotating disk 2, a group of sliding grooves 4 are opened on the top of the rotating disk 2, a group of sliding grooves 4 are slidably connected to a fixed block 5 through a sliding block, a second C-shaped tube 6 is fixedly connected to one side of the fixed block 5, a moving part is provided on the rotating disk 2, and the moving part is used to drive a group of second C-shaped tubes 6 to move, and a power part is provided at the bottom of the bottom shell 1, and the power part is used to drive the rotating disk 2 to rotate. When working, a group of second C-shaped tubes 6 are driven to move by the moving part, and the spacing between the first C-shaped tube 3 and the second C-shaped tube 6 can be adjusted, so as to fix centrifuge tubes of different sizes, and the rotating disk 2 is driven to rotate by the power part to perform centrifugal operation, thereby avoiding the prior art that only a single size of centrifuge tubes can be applied, which limits the requirements of the experiment, is not convenient to use, and requires the staff to replace a suitable centrifuge, which affects the efficiency of exosome extraction.

[0024] The movement includes a first through slot 7, a group of first through slots 7 are respectively opened on a group of fixed blocks 5, the rotating disk 2 is fixedly connected with an electric telescopic rod 8 through a fixed through hole, the telescopic end of the electric telescopic rod 8 is fixedly connected with a circular block 9, a group of second through slots 10 are opened on the edge of the circular block 9, a group of the first through slots 7 relative side walls are rotatably connected with a connecting rod 11 through a first rotating shaft, and the other end of a group of the connecting rods 11 are rotatably connected between a group of relative side walls of the second through slots 10 through a second rotating shaft. When working, the circular block 9 is driven to rise and fall by the electric telescopic rod 8, and the sliding block can be driven to slide and connect in the sliding slot 4 through the connection of the connecting rod 11, so that the second C-shaped tube 6 can be driven to be adjusted according to the diameter of the centrifuge tube, so that it can be suitable for most centrifuge tube sizes, avoiding the need for staff to replace the centrifuge and reduce the extraction efficiency.

[0025] The power part includes a motor 12, which is fixed to the inner circular wall of the bottom shell 1 through a circular rod. A driving rod 13 is provided at the output end of the motor 12. A first gear 14 is fixed to the bottom of the driving rod 13, and the first gear 14 is rotatably connected to the inner bottom wall of the bottom shell 1 through a third rotating shaft. A second gear 15 is fixed to the bottom of the electric telescopic rod 8, and the second gear 15 is rotatably connected to the inner bottom wall of the bottom shell 1 through a fourth rotating shaft. The first gear 14 and the second gear 15 are meshed and connected with each other. When working, the driving rod 13 is driven to rotate by the motor 12, and the first gear 14 is driven to rotate by the driving rod 13, so that the second gear 15 is driven to rotate by the first gear 14, so that the electric telescopic rod 8 fixed to the rotating disk 2 can be driven to rotate, so that the centrifugal operation of the centrifuge tube can be performed.

[0026] A group of square rods 16 are slidably connected to the first C-shaped tube 3 through a group of square through holes, one end of the square rods 16 is fixedly connected to an extrusion plate 17, and the extrusion plate 17 is located on the inner side of the first C-shaped tube 3, and the other end of the square rods 16 is fixedly connected to a square block 18, and a threaded rod 19 is threadedly connected to the square block 18 through a threaded hole, one end of the threaded rod 19 is rotatably connected to the outer circular wall of the first C-shaped tube 3, and the other end of the threaded rod 19 is fixedly connected to a rotary valve 20. During operation, through the provided threaded rods 19 and the extrusion plate 17, when the staff needs to perform centrifugal operation on a centrifuge tube with a smaller diameter, they can rotate the rotary valve 20 to squeeze the centrifuge tube with a smaller diameter through the extrusion plate 17 to achieve a fixing effect, so that the extraction device can adapt to centrifuge tubes of various sizes, and can avoid the problem that when the centrifuge tube has a small diameter and is not well matched, the centrifuge tube may be subjected to excessive force and rupture during high-speed rotation.

[0027] The top of the circular block 9 is fixedly connected to a first fixing rod 21, and the top of the first fixing rod 21 is fixedly connected to a top cover 22. During operation, the top of the circular block 9 is fixedly connected to the first fixing rod 21 and the top cover 22. After the centrifuge tube body is squeezed, the top of a group of centrifuge tubes can be squeezed through the top cover 22, so that the centrifuge tubes can remain stable when rotating rapidly, thereby increasing their stabilizing effect.

[0028] The first C-shaped tube 3, the second C-shaped tube 6 and the extrusion plate 17 are all provided with rubber pads on their inner sides. When working, the first C-shaped tube 3, the second C-shaped tube 6 and the extrusion plate 17 are all provided with rubber pads on their inner sides, which can play a protective role when clamping the centrifuge tube, and can increase the stability of the centrifuge tube through the friction force of the rubber pads.

[0029] Working principle: A group of second C-shaped tubes 6 are driven to move by a moving part, and the spacing between the first C-shaped tube 3 and the second C-shaped tube 6 can be adjusted, so as to fix centrifuge tubes of different sizes, and the rotating disk 2 is driven to rotate by a power part to perform a centrifugal operation, thereby avoiding the prior art that can only be used for centrifuge tubes of a single size, which limits the requirements of the experiment and is not convenient to use. The staff needs to replace a suitable centrifuge, which affects the efficiency of exosome extraction. The circular block 9 is driven to rise and fall by an electric telescopic rod 8, and the sliding block can be driven to slide and connect in the sliding groove 4 through the connection of the connecting rod 11, so as to drive the second C-shaped tube 6 to adjust according to the diameter of the centrifuge tube, so as to be suitable for most centrifuge tube sizes, avoiding the need for staff to replace the centrifuge and reduce the extraction efficiency. The driving rod 13 is driven to rotate by a motor 12, and the first gear 14 is driven to rotate by the driving rod 13, so that the second gear 15 is driven to rotate by the first gear 14, which can drive the electric motor fixed on the rotating disk 2. The telescopic rod 8 is movable to rotate, so that the centrifugal operation of the centrifuge tube can be performed. The threaded rod 19 and the extrusion plate 17 are set, so that when the staff needs to perform centrifugal operation on the centrifuge tube with a smaller diameter, the centrifuge tube with a smaller diameter can be squeezed by the extrusion plate 17 by rotating the rotating valve 20 to achieve a fixing effect, so that the extraction device can adapt to centrifuge tubes of various sizes, and can avoid the problem that the centrifuge tube may be subjected to excessive force and rupture during high-speed rotation when the centrifuge tube diameter is small and not matched enough. The first fixing rod 21 and the top cover 22 are fixedly connected to the top of the circular block 9. After the centrifuge tube body is squeezed, the top of a group of centrifuge tubes can be squeezed through the top cover 22, so that the centrifuge tube can remain stable when rotating at high speed, thereby increasing its stabilizing effect. The first C-shaped tube 3, the second C-shaped tube 6 and the inner side of the extrusion plate 17 are all provided with rubber pads, which can play a protective role when clamping the centrifuge tube, and the stability of the centrifuge tube can be increased by the friction of the rubber pads.

[0030] The above-mentioned front, back, left, right, top and bottom are all based on the figures in the specification. Figure 1 As a benchmark, according to the person's observation perspective, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0031] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the scope of protection of the present invention.

[0032] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A highly applicable exosome extraction device, characterized in that: The invention comprises a bottom shell (1), wherein a rotating disk (2) is rotatably connected to the inner circular wall of the bottom shell (1) via a rotating groove, a group of first C-shaped tubes (3) are fixedly connected to the top of the rotating disk (2), a group of sliding grooves (4) are opened on the top of the rotating disk (2), a fixed block (5) is slidably connected to the inside of each group of sliding grooves (4) via a sliding block, a second C-shaped tube (6) is fixedly connected to one side of the fixed block (5), a moving part is provided on the rotating disk (2), and the moving part is used to drive the group of second C-shaped tubes (6) to move, and a power part is provided at the bottom of the bottom shell (1), and the power part is used to drive the rotating disk (2) to rotate.

2. The highly applicable exosome extraction device according to claim 1, characterized in that: The movement comprises a first through slot (7), a group of first through slots (7) are respectively opened on a group of fixed blocks (5), the rotating disk (2) is fixedly connected to an electric telescopic rod (8) through a fixed through hole, the telescopic end of the electric telescopic rod (8) is fixedly connected to a circular block (9), the edge of the circular block (9) is provided with a group of second through slots (10), a group of the first through slots (7) are rotatably connected to the opposite side walls by a first rotating shaft with a connecting rod (11), and the other ends of the connecting rods (11) are respectively rotatably connected to the opposite side walls of the second through slots (10) by a second rotating shaft.

3. A highly applicable exosome extraction device according to claim 2, characterized in that: The power member comprises a motor (12), the motor (12) being fixedly connected to the inner circular wall of the bottom shell (1) via a circular rod, a driving rod (13) being provided at the output end of the motor (12), a first gear (14) being fixedly connected to the bottom of the driving rod (13), the first gear (14) being rotatably connected to the inner bottom wall of the bottom shell (1) via a third rotating shaft, a second gear (15) being fixedly connected to the bottom of the electric telescopic rod (8), the second gear (15) being rotatably connected to the inner bottom wall of the bottom shell (1) via a fourth rotating shaft, the first gear (14) and the second gear (15) being meshed and connected with each other.

4. The highly applicable exosome extraction device according to claim 3, characterized in that: A group of square rods (16) are slidably connected to the first C-shaped tube (3) through a group of square through holes, one end of the group of square rods (16) is fixedly connected to an extrusion plate (17), and the extrusion plate (17) is located inside the first C-shaped tube (3), the other end of the group of square rods (16) is fixedly connected to a square block (18), a threaded rod (19) is threadedly connected to the square block (18) through a threaded hole, one end of the threaded rod (19) is rotatably connected to the outer circular wall of the first C-shaped tube (3), and the other end of the threaded rod (19) is fixedly connected to a rotary valve (20).

5. The highly applicable exosome extraction device according to claim 4, characterized in that: A first fixing rod (21) is fixedly connected to the top of the circular block (9), and a top cover (22) is fixedly connected to the top of the first fixing rod (21).

6. The highly applicable exosome extraction device according to claim 5, characterized in that: Rubber pads are provided on the inner sides of the first C-shaped tube (3), the second C-shaped tube (6) and the extrusion plate (17).

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