Biological reagent sample application device

Abstract

The utility model provides a biological reagent sample application device and belongs to the technical field of biological sample application. The problem that it is troublesome and laborious to apply various stock solutions to the same membrane sample is solved. The biological reagent sample application device comprises a working table used for placing a sample, a supporting frame is further arranged in the middle of the working table and divides the working table into a sample application area and a reagent stock solution extraction area, a sample application platform is arranged at the sample application area and movably connected to the supporting frame, a sample application box is further arranged on the supporting frame, and the sample application box is movably connected to the supporting frame. An injection nozzle capable of injecting a stock solution is arranged on one side of the sample application box, a storage shell for containing the stock solution is arranged on the other side of the sample application box, and an output assembly capable of quantitatively conveying the stock solution in the storage shell to the injection nozzle is arranged in the sample application box. The injection nozzles at the two ends of the sample application box are connected with the storage shell through the output assembly, the stock solution can be extracted through the output assembly, and quantitative sample application injection is conducted according to the needed injection amount.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological spotting technology and relates to a biological reagent spotting device. Background Art

[0002] In clinical testing, there may be many pathogens that cause patients to become ill, such as bacteria, viruses, fungi, Pneumocystis, Toxoplasma, Chlamydia and Plasmodium, etc. In order to determine the true pathogen, different reagents need to be used for separate testing. In the detection of epidemic viruses, it is often necessary to detect and compare the specific nucleotide sequence of the virus, and different reagents need to be used for different specific target sequences.

[0003] Used for spotting of biological reagents, the spotted reagent membrane will produce different color changes after the reaction to achieve the test effect.

[0004] Bioreactor chip spotting technology can be divided into probe spotting technology and print head spotting technology. In the probe spotting technology, each detection needle can only spray one reagent. The print head spotting technology mainly includes piezoelectric inkjet printing technology, which is suitable for bioreactor chip spotting.

[0005] The current actual spotting is generally done manually by operating a spotting syringe to extract the reagent stock solution and then inject it into the container. This is difficult when a certain amount of stock solution needs to be injected in large batches. In addition, most of the probes are controlled manually, and the spotting efficiency is not high. Utility Model Content

[0006] The purpose of the utility model is to provide a biological reagent spotting device with a compact structure and strong stability in view of the above problems existing in the prior art.

[0007] The purpose of the utility model can be achieved through the following technical solutions:

[0008] A biological reagent spotting device comprises a workbench for placing samples, characterized in that a support frame is also provided in the middle of the workbench, the support frame divides the workbench into a spotting area and an area for extracting reagent stock solution, a spotting platform is provided in the spotting area, the spotting platform is movably connected to the support frame, a spotting box is also provided on the support frame, an injection nozzle capable of injecting stock solution is provided on one side of the spotting box, a storage shell for containing stock solution is provided on the other side, and an output component capable of quantitatively transporting the stock solution in the storage shell to the injection nozzle is provided inside the spotting box.

[0009] The workbench is divided into two areas by a support frame, one is a spotting area, and the other is an area for extracting reagent stock solution. The spotting platform is located in the spotting area of ​​the workbench, and the spotting platform is movably connected to the support frame. A spotting box is also arranged on the support frame. An injection nozzle capable of injecting stock solution is arranged on one side of the spotting box, and a storage shell for containing stock solution is arranged on the other side. The injection nozzle faces the spotting platform below. The spotting platform can be moved to the bottom of the injection nozzle so that the injection nozzle micro-contacts the spotting membrane of the spotting platform for spotting. The output component connects the injection nozzles at both ends of the spotting box with the storage shell, and the stock solution can be extracted through the output component and quantitative spotting injection can be performed according to the required injection volume.

[0010] In the above-mentioned biological reagent spotting device, the output component includes a metering pump, a hose, and a delivery pump. The hose includes an output tube and an input tube. The input tube is connected to the input end of the metering pump. One end of the output tube is connected to the output end of the metering pump, and the other end is connected to the injection nozzle. The delivery pump is connected to the tail end of the output tube, and the delivery pump is arranged in a storage shell.

[0011] The reagent stock solution is stored inside the storage shell, and the stock solution can be quantitatively delivered to the injection nozzle through the metering pump and the delivery pump, and then sprayed out by the injection nozzle. That is to say, according to the required injection volume, the delivery pump and the metering pump can extract the required injection volume, and then the metering pump outputs the required injection volume of stock solution to the injection nozzle.

[0012] In the above-mentioned biological reagent spotting device, the support frame is further provided with a guide rail, both ends of the spotting platform are further provided with sliders, the lower part of the workbench is further provided with a driver, and the output end of the driver extends out of the workbench and is connected to the lower surface of the spotting platform.

[0013] The guide rails are respectively located on both sides of the support frame, that is, the guide rails are located on both sides of the spotting box. Sliders are connected to both ends of one side of the spotting platform. The slider matches the guide rails on the support frame. A hole for the output end of the driver to extend is opened on the workbench. The driver is installed at the lower part of the workbench. The spotting platform can be driven up or down by the driver, that is to say, the spotting platform can be close to the injection nozzle on the spotting box through the driver.

[0014] In the above-mentioned biological reagent spotting device, a transparent protective cover is also provided at the end of the spotting box, a connecting groove is also provided at the lower surface of the injection nozzle, the injection nozzle is located at the center of the connecting groove, and both ends of the transparent protective cover are open, one end is connected to the connecting groove, and the other end extends out of the injection nozzle.

[0015] A connecting groove is provided at the end of the spotting box, that is, the spotting box located directly above the spotting platform. The connecting groove faces the spotting platform. A transparent protective cover is connected in the connecting groove. Both ends of the transparent protective cover are open, and the injection is located in the transparent protective cover. One end of the transparent protective cover is connected to the connecting groove, and the other end extends out of the injection nozzle to play a protective role.

[0016] In the above-mentioned biological reagent spotting device, the spotting platform is provided with a placement groove for placing the spotting membrane, and the spotting platform is also provided with an annular groove, which is located outside the placement groove, and the size of the annular groove matches the size of the transparent protective cover.

[0017] A plurality of placement grooves are provided on the spotting platform, and an annular groove is provided at the edge of the placement groove. The annular groove is located directly below the transparent protective cover, and the size of the annular groove is consistent with the size of the transparent protective cover. That is to say, when the spotting platform is driven to move upward, the end of the transparent protective cover is embedded in the annular groove, and the injection nozzle located in the transparent protective cover can be located in the spotting membrane at the placement groove.

[0018] In the above-mentioned biological reagent spotting device, the injection nozzle, quantitative pump, output tube, input tube, and delivery pump on the spotting box form a delivery unit, and the delivery unit is evenly arranged along the length direction of the spotting box. The transparent protective cover at the injection nozzle and the grooves and annular grooves placed on the spotting platform form an injection protection unit, and the number of the injection protection units corresponds to the number of the delivery units.

[0019] Multiple delivery units and multiple injection protection units can increase the sampling efficiency and improve the sampling rate, and can perform multiple groups of simultaneous spraying of quantitative stock solutions and micro-contact with the membrane at the sampling membrane to complete the reagent sampling.

[0020] In the above-mentioned biological reagent spotting device, a plurality of U-shaped spacer bars are further arranged inside the storage shell, and the plurality of spacer bars are evenly spaced at the bottom of the storage shell, and a snap-in groove is opened on the spacer bar, and a spacer plate is embedded in the snap-in groove, and the storage shell is divided into a plurality of chambers by the spacer plates, and each chamber corresponds to a conveying unit.

[0021] The storage shell is divided into multiple chambers by multiple spacer bars and spacer plates, and each chamber corresponds to a transport unit, so that different reagent stock solutions can be placed in different chambers, and the membranes at each group of corresponding spotting membranes can be spotted. According to the required requirements, the reagent stock solution can be extracted and then spotted on the spotting membranes, and a batch of the same spotting membranes can be spotted using different reagent stock solutions.

[0022] Compared with the prior art, the biological reagent spotting device is provided with a support frame in the middle of the working table, on which a spotting box is installed, so as to divide the working table into a spotting area and an area for extracting reagent stock solution, and a movable spotting platform is provided in the spotting area, one side of the spotting box is provided with an injection nozzle capable of injecting stock solution, and the other side is provided with a storage shell for containing stock solution, the injection nozzle is directly facing the spotting platform below, and the spotting platform can be driven to move up or down by a driver, and after moving up, the spotting membrane sheet placed in the groove on the spotting platform is brought close to the injection nozzle on the spotting box, and a plurality of conveying units and a plurality of injection protection units are provided at the spotting box, so as to increase the spotting efficiency and improve the spotting rate, and can perform multiple groups of simultaneous spraying of quantitative stock solution and micro-contact with the membrane sheet at the spotting membrane to complete the reagent spotting. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The present invention is a schematic diagram of the structure of a working platform in a biological reagent spotting device.

[0024] Figure 2 The present invention is a schematic diagram of the structure of a storage shell in a biological reagent spotting device.

[0025] Figure 3 The present invention is a structural schematic diagram of a biological reagent spotting device.

[0026] Figure 4 The present invention is a structural schematic diagram of a guide rail and a spotting platform in a biological reagent spotting device.

[0027] Figure 5 The present invention is a structural schematic diagram of a quantitative pump, a hose and a delivery pump at a spotting box in a biological reagent spotting device.

[0028] Figure 6 The present invention is a schematic diagram of the structure of a placement groove of a spotting platform in a biological reagent spotting device.

[0029] In the figure, 1. workbench; 2. support frame; 3. spotting area; 4. reagent stock solution extraction area; 5. spotting box; 6. injection nozzle; 7. storage shell; 8. quantitative pump; 9. spotting platform; 10. delivery pump; 11. input pipe; 12. output pipe; 13. driver; 14. guide rail; 15. slider; 16. transparent protective cover; 17. placement groove; 18. annular groove; 19. spacer bar; 20. snap-in groove; 21. spacer plate; 22. chamber. DETAILED DESCRIPTION

[0030] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6As shown, the biological reagent spotting device comprises a workbench 1 for placing samples, a support frame 2 is further provided in the middle of the workbench 1, the support frame 2 divides the workbench 1 into a spotting area 3 and a reagent stock solution extraction area 4, a spotting platform 9 is provided at the spotting area 3, the spotting platform 9 is movably connected to the support frame 2, a spotting box 5 is also provided on the support frame 2, an injection nozzle 6 capable of injecting stock solution is provided on one side of the spotting box 5, a storage shell 7 for containing stock solution is provided on the other side, and an output component capable of quantitatively transporting the stock solution in the storage shell 7 to the injection nozzle 6 is provided inside the spotting box 5.

[0031] The output component includes a metering pump 8, a hose, and a delivery pump 10. The hose includes an output pipe 12 and an input pipe 11. The input pipe 11 is connected to the input end of the metering pump 8. One end of the output pipe 12 is connected to the output end of the metering pump 8, and the other end is connected to the injection nozzle 6. The delivery pump 10 is connected to the tail end of the output pipe 12, and the delivery pump 10 is arranged in the storage shell 7.

[0032] The support frame 2 is further provided with a guide rail 14 , both ends of the spotting platform 9 are further provided with sliders 15 , a driver 13 is further provided at the lower part of the workbench 1 , and the output end of the driver 13 extends out of the workbench 1 and is connected to the lower surface of the spotting platform 9 .

[0033] A transparent protective cover 16 is also provided at the end of the spotting box 5, and a connecting groove is also provided on the lower surface of the injection nozzle 6. The injection nozzle 6 is located at the center of the connecting groove. Both ends of the transparent protective cover 16 are open, and one end is connected to the connecting groove, and the other end extends out of the injection nozzle 6.

[0034] The spotting platform 9 is provided with a placement groove 17 for placing the spotting membrane, and the spotting platform 9 is also provided with an annular groove 18 which is located outside the placement groove 17 and has a size matching that of the transparent protective cover 16 .

[0035] The injection nozzle 6, the quantitative pump 8, the output tube 12, the input tube 11, and the delivery pump 10 on the spotting box 5 form a delivery unit, and the delivery units are evenly arranged along the length direction of the spotting box 5. The transparent protective cover 16 at the injection nozzle 6 and the groove 17 and the annular groove 18 placed on the spotting platform 9 form an injection protection unit, and the number of the injection protection units corresponds to the number of the delivery units.

[0036] A plurality of U-shaped spacer bars 19 are also provided inside the storage shell 7. The plurality of spacer bars 19 are evenly spaced at the bottom of the storage shell 7, and a snap-in groove is provided on the spacer bar 19. A spacer plate 21 is embedded in the snap-in groove. The storage shell 7 is divided into a plurality of chambers 22 by the spacer plates 21, and each chamber 22 corresponds to a conveying unit.

[0037] The workbench 1 is divided into two areas by a support frame 2, one is a sample spotting area 3, and the other is a reagent stock solution extraction area 4. The sample spotting platform 9 is located at the sample spotting area 3 of the workbench 1, and the sample spotting platform 9 is movably connected to the support frame 2. A sample spotting box 5 is also arranged on the support frame 2. An injection nozzle 6 capable of injecting stock solution is arranged on one side of the sample spotting box 5, and a storage shell 7 containing stock solution is arranged on the other side. The injection nozzle 6 is located above the sample spotting platform 9 in the sample spotting area 3, and the storage shell 7 is located above the reagent stock solution extraction area 4. The guide rails 14 are respectively located on both sides of the support frame 2, that is, the guide rails 14 are located on both sides of the spotting box 5. Slide blocks 15 are connected to both ends of one side of the spotting platform 9. The slide block 15 matches the guide rails 14 on the support frame 2. A hole for the output end of the driver 13 to extend is provided on the workbench 1. The driver 13 is installed at the lower part of the workbench 1. The spotting platform 9 can be driven to move up or down through the driver 13, that is, the spotting platform 9 can be close to the injection nozzle 6 on the spotting box 5 through the driver 13.

[0038] At the end of the spotting box 5, that is, the spotting box 5 located directly above the spotting platform 9, a connecting groove is also provided, the connecting groove is directly opposite to the spotting platform 9, a transparent protective cover 16 is also connected in the connecting groove, and both ends of the transparent protective cover 16 are open. A plurality of placement grooves 17 are also provided on the spotting platform 9, and an annular groove 18 is also provided at the edge of the placement groove 17, the annular groove 18 is located directly below the transparent protective cover 16, and the size of the annular groove 18 is consistent with the size of the transparent protective cover 16, that is, the injection injection is located in the transparent protective cover 16, one end of the transparent protective cover 16 is connected to the connecting groove, and the other end extends out of the injection nozzle 6, after the spotting platform 9 is driven upward by the driver 13, the end of the transparent protective cover 16 can be embedded in the annular groove 18, and the injection nozzle 6 can be in micro-contact with the spotting membrane in the placement groove 17 to complete the reagent spotting.

[0039] During spotting, the driver 13 can drive the spotting platform 9 to move upwards, close to the injection nozzle 6 under the spotting box 5. After moving upwards, the lower end of the transparent protective cover 16 on the spotting box 5 is embedded in the annular groove 18, and at the same time, the injection nozzle 6 micro-contacts the spotting membrane in the groove 17 to complete the spotting. After completion, the driver 13 can drive the spotting platform 9 to move downwards and reset, thereby completing multiple groups of spotting at one time.

[0040] The injection nozzle 6, the quantitative pump 8, the output tube 12, the input tube 11, and the delivery pump 10 on the spotting box 5 form a delivery unit, and the delivery unit is evenly arranged along the length direction of the spotting box 5. The transparent protective cover 16 at the injection nozzle 6 and the groove 17 and the annular groove 18 placed on the spotting platform 9 form an injection protection unit. The number of injection protection units corresponds to the number of delivery units. A plurality of the spacer bars 19 are evenly spaced at intervals at the bottom of the storage shell 7, and a card groove is opened on the spacer bar 19. A spacer plate 21 is embedded in the card groove. The storage shell 7 is divided into a plurality of chambers 22 by the spacer plate 21, and each chamber 22 is connected to a Corresponding to the conveying unit, the storage shell 7 is divided into multiple chambers 22 by multiple spacer bars 19 and spacer plates 21, and each chamber 22 corresponds to a conveying unit, so that different reagent stock solutions can be in different chambers 22, and the membranes at each corresponding spotting membrane can be spotted. According to the required requirements, the spotting membrane can be spotted after the reagent stock solution is extracted. A batch of the same spotting membranes can be spotted with different reagent stock solutions. Multiple conveying units and multiple injection protection units can increase the spotting efficiency and improve the spotting rate. Multiple groups of quantitative stock solutions can be sprayed out simultaneously and then micro-contacted with the membrane at the spotting membrane to complete the reagent spotting.

[0041] When in use, the spotting membrane is placed in the placement groove 17 of the spotting platform 9 according to the required reagent spotting, and the reagent stock solution corresponding to the spotting membrane is added to the chamber 22 of the storage shell 7, that is, the spotting membrane under the injection nozzle 6 and the spotting stock solution in the chamber 22 connected to the injection nozzle 6 are a group, and one conveying unit corresponds to one chamber 22. The stock solution required by the spotting membrane can be replaced, and multiple groups of quantitative stock solutions can be sprayed out simultaneously and then micro-contacted with the membrane at the spotting membrane to complete the reagent spotting, so that the same batch of spotting membranes can be used for spotting detection of different spotting reagents. It can increase the spotting efficiency and improve the spotting rate.

[0042] All technical features in this embodiment can be freely combined according to actual needs.

[0043] Finally, it should be noted that the above is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. A biological reagent spotting device, comprising a workbench for placing samples, characterized in that: A support frame is also provided in the middle of the workbench, and the support frame divides the workbench into a sampling area and an area for extracting reagent stock solution. A sampling platform is provided in the sampling area, and the sampling platform is movably connected to the support frame. A sampling box is also provided on the support frame. An injection nozzle capable of injecting stock solution is provided on one side of the sampling box, and a storage shell for containing stock solution is provided on the other side. An output component capable of quantitatively transporting the stock solution in the storage shell to the injection nozzle is provided inside the sampling box.

2. A biological reagent spotting device according to claim 1, characterized in that: The output component includes a metering pump, a hose, and a delivery pump. The hose includes an output pipe and an input pipe. The input pipe is connected to the input end of the metering pump. One end of the output pipe is connected to the output end of the metering pump, and the other end is connected to the injection nozzle. The delivery pump is connected to the tail end of the output pipe, and the delivery pump is arranged in a storage shell.

3. A biological reagent spotting device according to claim 2, characterized in that: The support frame is also provided with guide rails, both ends of the spotting platform are also provided with sliders, the lower part of the working table is also provided with a driver, and the output end of the driver extends out of the working table and is connected to the lower surface of the spotting platform.

4. A biological reagent spotting device according to claim 3, characterized in that: A transparent protective cover is also provided at the end of the spotting box, a connecting groove is also provided at the lower surface of the injection nozzle, the injection nozzle is located at the center of the connecting groove, both ends of the transparent protective cover are open, one end is connected to the connecting groove, and the other end extends out of the injection nozzle.

5. A biological reagent spotting device according to claim 4, characterized in that: The spotting platform is provided with a placement groove for placing the spotting membrane, and is also provided with an annular groove located outside the placement groove, and the size of the annular groove matches the size of the transparent protective cover.

6. A biological reagent spotting device according to claim 5, characterized in that: The injection nozzle, quantitative pump, output pipe, input pipe and delivery pump on the spotting box form a delivery unit, and the delivery units are evenly arranged along the length direction of the spotting box. The transparent protective cover at the injection nozzle and the grooves and annular grooves placed on the spotting platform form an injection protection unit, and the number of the injection protection units corresponds to the number of the delivery units.

7. A biological reagent spotting device according to claim 6, characterized in that: A plurality of U-shaped spacer bars are also provided inside the storage shell. The plurality of spacer bars are evenly spaced at the bottom of the storage shell, and a snap-in groove is opened on the spacer bar. A spacer plate is embedded in the snap-in groove. The storage shell is divided into a plurality of chambers by the spacer plates, and each chamber corresponds to a conveying unit.

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