Stem cell purification, extraction and separation device

Abstract

The utility model discloses a stem cell purification, extraction and separation device, relates to stem cell extraction technical field, including main box body and filter component, main box body outside is provided with box door, and box door inner upper end is provided with observation window, main box body upper end middle part is provided with centrifugal motor, main box body inner lower end is provided with filter component, and the main box body is provided with the filter component. The filtering assembly comprises a fixing block, a clamping sliding groove, a first cell screen, a mounting block, a pneumatic rod, a movable vertical plate, a cleaning scraper, a limiting cross rod, a second cell screen and a material collecting frame, the clamping sliding groove is formed in the fixing block, and the first cell screen is connected into the clamping sliding groove. According to the stem cell purification, extraction and separation device, through the arrangement of the filter assembly, the problem that when most separation devices extract stem cells, impurities in cell suspension are likely to precipitate on a filter screen, so that the filter screen is blocked, and the activity and quality of the stem cells are affected is solved, and the overall practicability of the separation device is conveniently improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell extraction, in particular to a stem cell purification, extraction and separation device. Background Art

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal ability and can produce at least one type of highly differentiated daughter cells. Most biologists and medical scientists believe that stem cells are a type of cell from embryos, fetuses or adults that has unlimited self-renewal and proliferation and differentiation ability under certain conditions. They can produce daughter cells with the same phenotype and genotype as themselves, and can also produce specialized cells that make up the body's tissues and organs. At the same time, they can also differentiate into progenitor cells. In order to improve the efficiency of stem cell purification and extraction, a separation device is needed, but the existing separation devices still have the following shortcomings:

[0003] For example, the utility model with application number 202222813346.6 discloses a device for extracting adipose stem cells by digesting adipose tissue. A second cell screen and a sterile polyethylene filter membrane are installed inside the separation and purifier of the utility model, and a fourth syringe connector is installed at one end of the separation and purifier. The problems of low efficiency and poor separation effect of existing fat particle purification devices are overcome to maximize the separation rate and culture efficiency of adipose stem cells. However, similar to the comparative documents of the above-mentioned application, when the existing separation devices are used, since impurities in the cell suspension of most separation devices are easily precipitated on the filter when extracting stem cells, the filter becomes clogged, thereby affecting the activity and quality of the stem cells, resulting in reduced practicality of the separation device and reduced utilization efficiency.

[0004] Therefore, in view of this, the existing structure and deficiencies are studied and improved, and a stem cell purification, extraction and separation device is proposed. Utility Model Content

[0005] The purpose of the utility model is to provide a stem cell purification, extraction and separation device to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a stem cell purification, extraction and separation device, comprising a main box and a filter assembly, a box door is arranged outside the main box, and an observation window is arranged at the upper end of the box door, a centrifugal motor is installed in the middle of the upper end of the main box, a filter assembly is arranged at the lower end of the main box, and the filter assembly comprises a fixed block, a card-connecting slide, a first cell screen, a mounting block, a pneumatic rod, a movable vertical plate, a cleaning scraper, a limiting cross bar, a second cell screen and an aggregate frame, a card-connecting slide is opened inside the fixed block, and the first cell screen is connected inside the card-connecting slide, a mounting block is arranged in the middle of the upper end of the fixed block, and a pneumatic rod is installed on the left side of the mounting block, and a movable vertical plate is installed at the front end of the pneumatic rod, and a cleaning scraper is installed at the lower end of the movable vertical plate, and limiting cross bars are connected on both sides of the movable vertical plate, a second cell screen is distributed at the lower end of the first cell screen, and an aggregate frame is arranged below the second cell screen, and a centrifugal assembly is arranged at the upper end of the main box.

[0007] Furthermore, the inner dimensions of the snap-fitting slide groove match the outer dimensions of the first cell screen, and the first cell screen is slidably connected to the fixed block via the snap-fitting slide groove.

[0008] Furthermore, the movable vertical plate and the first cell screen are vertically distributed, and the cleaning scraper is tightly fitted to the outer side of the first cell screen through the movable vertical plate.

[0009] Furthermore, the limiting cross bar is embeddedly connected to the movable vertical plate, and the movable vertical plate is slidably connected to the main box body through the limiting cross bar.

[0010] Furthermore, the centrifugal assembly includes a receiving block, a limiting ring, a limiting groove and a centrifugal bucket. The limiting ring is installed at the front end of the receiving block, and the limiting groove is opened inside the limiting ring, and the centrifugal bucket is connected inside the limiting ring.

[0011] Furthermore, the centrifugal assembly also includes a sealing cover, a limiting block and a discharge pipe. At the same time, a sealing cover is installed on the upper end of the centrifugal barrel, a limiting block is additionally provided on the outside of the centrifugal barrel, and a discharge pipe is provided in the middle of the lower end of the centrifugal barrel.

[0012] Furthermore, the limiting ring is embedded in the main box body, and the limiting ring is threadedly connected to the main box body through a receiving block.

[0013] Furthermore, the inner dimensions of the limiting groove match the outer dimensions of the limiting block, and the centrifugal bucket is rotatably connected to the limiting ring via the limiting block.

[0014] The utility model provides a stem cell purification, extraction and separation device, which has the following beneficial effects:

[0015] 1. The utility model can use the filter assembly arranged at the lower end of the main box body to prevent the impurities in the cell suspension from being easily precipitated on the filter net when most separation devices extract stem cells, thereby causing the filter net to be blocked, thereby affecting the activity and quality of the stem cells and causing the practicality of the separation device to decrease. The fixing block is fixedly installed on both sides of the main box body by fastening bolts, and the internal size of the clamping groove provided in the fixing block matches the external size of the first cell screen, and then the first cell screen is slidably installed into the fixing block by the clamping groove, and the fixing bolts are fastened to the fixing block by the clamping groove. The firmware fixes the mounting block in the middle of the upper end of the fixed block, and cooperates with the fasteners to fix the pneumatic rod on the outside of the mounting block. The operation of the pneumatic rod drives the movable vertical plate to move its position. At the same time, the limiting cross bars connected on both sides of the movable vertical plate limit the movement of the movable vertical plate, thereby increasing the stability of the movable vertical plate during movement. The cleaning scraper is fixedly installed at the lower end of the movable vertical plate. When the movable vertical plate moves, it drives the cleaning scraper to move on the surface of the first cell screen, thereby cleaning the first cell screen. The filtered stem cells fall onto the second cell screen for filtration again, and are finally collected by the aggregate frame, thereby improving the overall utilization efficiency of the separation device.

[0016] 2. The utility model can avoid the poor stability of the centrifugal bucket when most separation devices centrifuge stem cells through the centrifugal bucket through the centrifugal component arranged at the upper end of the main box body when the stem cell purification, extraction and separation device is used, which makes the purification of stem cells easily affected and reduces the use efficiency of the separation device. The receiving block is fixedly installed on both sides of the inside of the main box body through fasteners, and the limit ring is fixedly installed at the front end of the receiving block in conjunction with the fasteners. At the same time, the internal size of the limit ring matches the external size of the centrifugal bucket, and the internal size of the limit groove opened inside the limit ring matches the external size of the limit block added to the outside of the centrifugal bucket, so that the centrifugal bucket is limitedly installed inside the limit ring through the limit block and the limit groove, and the sealing cover is installed on the upper end of the centrifugal bucket. The output shaft of the centrifugal motor arranged at the upper end of the main box body is connected to the sealing cover through a coupling. The centrifugal motor drives the sealing cover to rotate the centrifugal bucket inside the main box body, so that the stem cell suspension added to the inside of the centrifugal bucket is separated, and discharged through the discharge pipe after the separation, further improving the use flexibility and dynamism of the separation device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall three-dimensional structure of a stem cell purification, extraction and separation device of the utility model;

[0018] Figure 2 This is a schematic diagram of the three-dimensional structure of a filter component of a stem cell purification, extraction and separation device of the utility model;

[0019] Figure 3 It is a schematic diagram of the three-dimensional structure of a centrifugal component of a stem cell purification, extraction and separation device of the utility model.

[0020] In the figure: 1. main box body; 2. box door; 3. observation window; 4. centrifugal motor; 5. filter assembly; 501. fixed block; 502. snap-on slide; 503. first cell screen; 504. mounting block; 505. pneumatic rod; 506. movable vertical plate; 507. cleaning scraper; 508. limiting cross bar; 509. second cell screen; 510. aggregate frame; 6. centrifugal assembly; 601. receiving block; 602. limiting ring; 603. limiting groove; 604. centrifugal barrel; 605. sealing cover; 606. limiting block; 607. discharge pipe. DETAILED DESCRIPTION

[0021] The following is a further detailed description of the implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0022] like Figure 1 and Figure 2As shown, a stem cell purification, extraction and separation device includes a main box body 1 and a filter assembly 5, wherein a box door 2 is arranged outside the main box body 1, and an observation window 3 is arranged at the upper end of the box door 2, a centrifugal motor 4 is installed in the middle of the upper end of the main box body 1, and a filter assembly 5 is arranged at the lower end of the main box body 1, and the filter assembly 5 includes a fixed block 501, a card slot 502, a first cell screen 503, a mounting block 504, a pneumatic rod 505, a movable vertical plate 506, a cleaning scraper 507, a limiting cross bar 508, a second cell screen 509 and a collection frame 510, a card slot 502 is opened inside the fixed block 501, and the card slot 502 is connected to the first cell screen 503, and the fixed block 50 A mounting block 504 is provided in the middle of the upper end, and a pneumatic rod 505 is installed on the left side of the mounting block 504, and a movable vertical plate 506 is installed at the front end of the pneumatic rod 505, and a cleaning scraper 507 is installed at the lower end of the movable vertical plate 506, and the two sides of the movable vertical plate 506 are connected to the limited horizontal rods 508, the lower end of the first cell screen 503 is distributed with a second cell screen 509, and a collection frame 510 is provided below the second cell screen 509, the inner size of the clamping slide 502 matches the outer size of the first cell screen 503, and the first cell screen 503 is slidably connected to the fixed block 501 through the clamping slide 502, and the movable vertical plate 506 and the first cell screen 503 are vertically distributed, The cleaning scraper 507 is tightly fitted with the outer side of the first cell screen 503 through the movable vertical plate 506, the limiting cross bar 508 is embedded with the movable vertical plate 506, and the movable vertical plate 506 is slidably connected with the main box body 1 through the limiting cross bar 508, and the fixing block 501 is fixedly installed on both sides of the inside of the main box body 1 through the fastening bolts, and the internal size of the clamping groove 502 opened in the fixing block 501 matches the external size of the first cell screen 503, and then the first cell screen 503 is slidably installed into the fixing block 501 through the clamping groove 502, and the mounting block 504 is fixedly installed in the middle of the upper end of the fixing block 501 through the fasteners, and the pneumatic rod 505 is fixedly installed with the fasteners. The pneumatic rod 505 is fixedly installed on the outside of the mounting block 504, and the operation of the pneumatic rod 505 drives the movable vertical plate 506 to move its position. At the same time, the limiting cross bars 508 connected on both sides of the movable vertical plate 506 limit the movement of the movable vertical plate 506, thereby increasing the stability of the movable vertical plate 506 during the movement. The cleaning scraper 507 is fixedly installed on the lower end of the movable vertical plate 506. When the movable vertical plate 506 moves, the cleaning scraper 507 is driven to move on the surface of the first cell screen 503, thereby cleaning the first cell screen 503. The filtered stem cells fall onto the second cell screen 509 for further filtration, and are finally collected by the aggregate frame 510, thereby improving the overall use efficiency of the separation device;

[0023] like Figure 1 and Figure 3As shown, a centrifugal assembly 6 is arranged at the upper end of the main box body 1, and the centrifugal assembly 6 includes a receiving block 601, a limiting ring 602, a limiting groove 603 and a centrifugal bucket 604. The limiting ring 602 is installed at the front end of the receiving block 601, and the limiting groove 603 is arranged inside the limiting ring 602, and the centrifugal bucket 604 is connected to the limiting ring 602. The centrifugal assembly 6 also includes a sealing cover 605, a limiting block 606 and a discharge pipe 607, and the upper end of the centrifugal bucket 604 is installed with a The centrifugal bucket 604 is provided with a sealing cover 605, a limiting block 606 is additionally provided on the outside, and a discharge pipe 607 is provided at the middle of the lower end of the centrifugal bucket 604, the limiting ring 602 is embeddedly connected with the main box body 1, and the limiting ring 602 is threadedly connected with the main box body 1 through the receiving block 601, the inner size of the limiting groove 603 matches the outer size of the limiting block 606, and the centrifugal bucket 604 is rotatably connected with the limiting ring 602 through the limiting block 606, and the receiving block 601 is fixed by fasteners. The centrifugal bucket 604 is fixedly installed on both sides of the interior of the main housing 1, and the limiting ring 602 is fixedly installed at the front end of the receiving block 601 with the fasteners. At the same time, the internal size of the limiting ring 602 matches the external size of the centrifugal bucket 604, and the internal size of the limiting groove 603 opened in the limiting ring 602 matches the external size of the limiting block 606 added to the outside of the centrifugal bucket 604, so that the centrifugal bucket 604 is limitedly installed inside the limiting ring 602 by the limiting block 606 cooperating with the limiting groove 603, and the sealing cover 605 is installed on the upper end of the centrifugal bucket 604. The output shaft of the centrifugal motor 4 arranged at the upper end of the main housing 1 is connected with the sealing cover 605 through a coupling. The operation of the centrifugal motor 4 drives the sealing cover 605 to make the centrifugal bucket 604 rotate inside the main housing 1, thereby separating the stem cell suspension added to the centrifugal bucket 604, and discharging it through the discharge pipe 607 after the separation, further improving the flexibility and dynamism of the separation device.

[0024] In summary, when the stem cell purification, extraction and separation device is used, first, the observation window 3 added on the outer door 2 of the main box body 1 is used to observe the situation inside the main box body 1 when the separation device is in use, and the centrifugal motor 4 is fixedly installed on the upper end of the main box body 1 through the fastening bolts, and the receiving block 601 is fixedly installed on both sides of the inside of the main box body 1 through the fasteners, and the limiting ring 602 is fixedly installed on the front end of the receiving block 601 with the fasteners, and the internal size of the limiting ring 602 matches the external size of the centrifugal bucket 604, and the inner size of the limiting groove 603 opened inside the limiting ring 602 is The size of the inner part matches the outer size of the limit block 606 added to the outside of the centrifugal bucket 604, so that the centrifugal bucket 604 is limitedly installed inside the limit ring 602 through the limit block 606 cooperating with the limit groove 603, and the sealing cover 605 is installed on the upper end of the centrifugal bucket 604. The output shaft of the centrifugal motor 4 arranged at the upper end of the main box body 1 is connected to the sealing cover 605 through a coupling. The centrifugal motor 4 drives the sealing cover 605 to make the centrifugal bucket 604 rotate inside the main box body 1, so as to separate the stem cell suspension added to the centrifugal bucket 604, and after the separation, the stem cells are separated by The material is discharged through the discharge pipe 607, and then the fixing block 501 is fixedly installed on both sides of the main box body 1 through the fastening bolts, and the internal size of the clamping slot 502 opened in the fixing block 501 matches the external size of the first cell screen 503, and then the first cell screen 503 is slidably installed into the fixing block 501 through the clamping slot 502, and the mounting block 504 is fixedly installed in the middle of the upper end of the fixing block 501 through the fasteners, and the pneumatic rod 505 is fixedly installed on the outside of the mounting block 504 with the fasteners, and the pneumatic rod 505 is operated to drive the movable vertical plate 506 to move in position. The movable vertical plate 506 moves, and at the same time, the limiting cross bars 508 connected on both sides of the movable vertical plate 506 limit the movement of the movable vertical plate 506, so as to increase the stability of the movable vertical plate 506 during the movement, and the cleaning scraper 507 is fixedly installed on the lower end of the movable vertical plate 506. When the movable vertical plate 506 moves, the cleaning scraper 507 is driven to move on the surface of the first cell screen 503, so as to clean the first cell screen 503, and the filtered stem cells fall onto the second cell screen 509 for filtration again, and are finally collected by the aggregate frame 510, so as to improve the overall use efficiency of the separation device.

[0025] The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

Claims

1. A stem cell purification, extraction and separation device, comprising a main housing (1) and a filter assembly (5), characterized in that: The main housing (1) is provided with a housing door (2) on the outside, and an observation window (3) is provided on the upper end of the housing door (2). A centrifugal motor (4) is installed in the middle of the upper end of the main housing (1). A filter assembly (5) is provided on the lower end of the main housing (1). The filter assembly (5) comprises a fixed block (501), a snap-fitting slide groove (502), a first cell screen (503), a mounting block (504), a pneumatic rod (505), a movable vertical plate (506), a cleaning scraper (507), a limiting cross bar (508), a second cell screen (509) and a collection frame (510). The fixed block (501) is provided with a snap-fitting slide groove (502) on the inside, and the snap-fitting slide groove (502) is provided on the inside. 02) is internally connected with a first cell screen (503), a mounting block (504) is arranged in the middle of the upper end of the fixed block (501), and a pneumatic rod (505) is installed on the left side of the mounting block (504), and a movable vertical plate (506) is installed at the front end of the pneumatic rod (505), and a cleaning scraper (507) is installed at the lower end of the movable vertical plate (506), and limiting cross bars (508) are connected to both sides of the movable vertical plate (506), a second cell screen (509) is distributed at the lower end of the first cell screen (503), and a collection frame (510) is arranged below the second cell screen (509), and a centrifugal assembly (6) is arranged at the upper end of the main box (1).

2. A stem cell purification, extraction and separation device according to claim 1, characterized in that: The inner dimensions of the snap-fitting slide groove (502) match the outer dimensions of the first cell screen (503), and the first cell screen (503) is slidably connected to the fixing block (501) via the snap-fitting slide groove (502).

3. A stem cell purification, extraction and separation device according to claim 1, characterized in that: The movable vertical plate (506) and the first cell screen (503) are arranged vertically, and the cleaning scraper (507) is tightly fitted to the outer side of the first cell screen (503) through the movable vertical plate (506).

4. The stem cell purification, extraction and separation device according to claim 1, characterized in that: The limiting cross bar (508) is embeddedly connected to the movable vertical plate (506), and the movable vertical plate (506) is slidably connected to the main box body (1) via the limiting cross bar (508).

5. The stem cell purification, extraction and separation device according to claim 1, characterized in that: The centrifugal assembly (6) comprises a receiving block (601), a limiting ring (602), a limiting groove (603) and a centrifugal bucket (604); the limiting ring (602) is installed at the front end of the receiving block (601), the limiting groove (603) is provided inside the limiting ring (602), and the centrifugal bucket (604) is connected inside the limiting ring (602).

6. A stem cell purification, extraction and separation device according to claim 5, characterized in that: The centrifugal assembly (6) further comprises a sealing cover (605), a limiting block (606) and a discharge pipe (607); the sealing cover (605) is installed at the upper end of the centrifugal barrel (604); a limiting block (606) is additionally provided on the outside of the centrifugal barrel (604); and a discharge pipe (607) is provided at the middle part of the lower end of the centrifugal barrel (604).

7. The stem cell purification, extraction and separation device according to claim 6, characterized in that: The limiting ring (602) is embedded in the main box body (1), and the limiting ring (602) is threadedly connected to the main box body (1) via a receiving block (601).

8. The stem cell purification, extraction and separation device according to claim 6, characterized in that: The inner dimensions of the limiting groove (603) match the outer dimensions of the limiting block (606), and the centrifugal bucket (604) is rotatably connected to the limiting ring (602) via the limiting block (606).

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