Batch dyeing tool for ultrathin sections of transmission electron microscope

Abstract

The utility model discloses a batch dyeing tool for ultrathin sections of a transmission electron microscope, and mainly relates to batch dyeing of the ultrathin sections of the transmission electron microscope. Comprising a dyeing body, the dyeing body is provided with a plurality of carrying net positioning seams matched with carrying nets in size, and the carrying nets are clamped on the carrying net positioning seams respectively. The utility model has the beneficial effects that a plurality of carrying nets can be dyed and cleaned at the same time at one time, so that the experiment efficiency is greatly improved, the labor intensity is reduced, and meanwhile, the dyeing time and the cleaning time of each carrying net can be ensured to be consistent; the tooth wax is selected as a whole manufacturing material through multiple tests, the tooth wax can keep a soft state at normal temperature, has moldability, is insoluble in water, is not easy to react with dye liquor, does not adsorb impurities in air, does not need to be cleaned and has an anti-pollution effect, the tooth wax does not pollute the dye liquor, and the cost of the material is extremely low.

Description

Technical Field

[0001] The utility model relates to the field of batch staining of ultrathin sections under a transmission electron microscope, in particular to a batch staining tool for ultrathin sections under a transmission electron microscope. Background Art

[0002] A transmission electron microscope (TEM) is a microscope that uses electrons as a "light source" to obtain high-resolution images of samples by penetrating the sample with an electron beam. TEM can provide higher resolution than an optical microscope, allowing researchers to observe structures at the nanoscale and even atomic level. Since its invention in the 1930s, this microscope has become an important tool for research in fields such as materials science, biology, and physics.

[0003] When using, first cut the tissue to be observed into very thin slices with a thickness between 60nm and 90nm, and then place the slices on the dedicated grid of the transmission electron microscope (hereinafter referred to as the electron microscope). The diameter of the grid dedicated to the electron microscope is about 3mm, and the thickness is only 20um. The outer edge of the grid is less than 0.5mm, and the whole grid is very small. The whole test process needs to be completed with the help of tweezers. After sticking the slices to the grid (as shown in the attached Fig. 9 As described above), after drying, the slices are fixed on the grid. Next, in order to make the structure of the slices clearly displayed under the electron microscope, we need to dye the slices with a heavy metal salt solution. After dyeing, it is usually necessary to use distilled water to clean the slices on the grid. After cleaning, put them under the electron microscope for observation to obtain a high-resolution image of the sample.

[0004] The most commonly used staining method in the laboratory requires the use of tweezers to clamp the grid and perform the operation step by step. Repeated clamping of the grid (usually made of copper or nickel) will damage the grid itself. At the same time, repeated clamping of ultra-thin slices will also cause damage to the nano-scale slices. In order to obtain accurate data, we often need to conduct multiple experiments and multiple observations to obtain accurate results. In the above-mentioned process of clamping one slice at a time with tweezers, the staining time of each grid cannot be kept consistent, and the staining of each grid lasts for about 20 minutes, which will make it impossible to accurately control the same variables, resulting in inaccurate subsequent experimental results. At the same time, the entire experimental process is very painful for the experimenters; and the staining process also needs to be carried out in a sealed, light-shielded environment, such as Fig.11 As mentioned above, the overall experimental efficiency is very low. Utility Model Content

[0005] In view of the above-mentioned problems, the purpose of the utility model is to provide a transmission electron microscope ultra-thin section batch staining tool, which can realize the simultaneous staining and cleaning of multiple grids at one time, greatly speeding up the efficiency of the experiment, reducing labor intensity, and also ensuring that the staining and cleaning time of each grid is consistent; and the utility model selects tooth wax as the overall manufacturing material after many experiments, which can maintain a soft state at room temperature, has plasticity, is insoluble in water, and is not easy to react with dye solution, does not absorb impurities in the air, has the effect of not needing to be cleaned and anti-pollution, and the tooth wax itself will not pollute the dye solution, and the dyeing process can be referred to as tooth wax. Fig.12 As described above, the whole tooth wax is placed in a special container, sealed, and then placed in a dark environment, which greatly speeds up the dyeing speed and the cost of the material itself is extremely low.

[0006] In order to achieve the above purpose, the utility model is implemented through the following technical solutions:

[0007] A transmission electron microscope ultra-thin section batch staining tool comprises a staining body, on which a plurality of grid positioning slits are provided in accordance with the size of a grid, and a plurality of grids are respectively clamped on the grid positioning slits.

[0008] The material of the dyeing body is a carrier mesh of dental wax material. The positioning gap is squeezed by squeezing the dental wax, and then the carrier mesh is squeezed and fixed on the positioning gap.

[0009] Support columns are arranged at both ends of the tooth wax, and the material of the support columns is wooden support columns.

[0010] A spine is arranged inside the dyeing body.

[0011] Compared with the prior art, the beneficial effects of the present invention are:

[0012] The utility model can dye and clean a plurality of carrier nets at one time, which greatly speeds up the efficiency of the experiment, reduces the labor intensity, and can also ensure that the dyeing and cleaning time of each carrier net are consistent; and the utility model selects tooth wax as the overall manufacturing material after many tests. The tooth wax can keep soft at room temperature, has plasticity, is insoluble in water, is not easy to react with dye solution, does not absorb impurities in the air, has the effects of not needing to be cleaned and being anti-pollution, and the tooth wax itself will not pollute the dye solution, and the material cost itself is extremely low. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Attached Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0014] Attached Figure 2 This utility model is attached Figure 1 Circle the image to enlarge.

[0015] Attached Figure 3 This utility model is attached Figure 1 Schematic diagram of the usage process.

[0016] Attached Figure 4 This utility model is attached Figure 1 Enlarged picture of the use process.

[0017] Attached Figure 5 It is a schematic diagram of the overall structure of Example 2 of the present utility model.

[0018] Attached Figure 6 It is a diagram of the overall structure use process of Example 2 in the utility model.

[0019] Attached Figure 7 This is the practical operation of the utility model embodiment 1 Figure 1 .

[0020] Attached Figure 8 This is the practical operation of the utility model embodiment 1 Figure 2 .

[0021] Attached Fig. 9 It is a schematic diagram of the network carrier of the utility model.

[0022] Attached Fig.10 It is a piece-by-piece operation diagram of the traditional working method.

[0023] Attached Fig.11 It is the traditional preparation before dyeing.

[0024] Attached Fig.12 It is the preparation for dyeing work of the utility model.

[0025] Numbers shown in the accompanying drawings:

[0026] 1. Dyeing body; 2. Carrier mesh positioning seam; 3. Support column; 4. Spine. DETAILED DESCRIPTION

[0027] The present invention is further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the present application.

[0028] The utility model is a transmission electron microscope ultra-thin section batch staining tool, please refer to the attached Figure 1-5The overall structure includes a dyeing body 1, the material of the dyeing body 1 is a tooth wax material carrier mesh, and the dyeing body 1 is provided with a plurality of carrier mesh positioning slits 2 in accordance with the size of the carrier mesh, and the plurality of carrier meshes are respectively clamped on the carrier mesh positioning slits 2. Because the tooth wax material itself is very soft and will be deformed when squeezed, the positioning slits can be squeezed by squeezing the tooth wax, and then the carrier mesh can be squeezed and fixed on the positioning slits.

[0029] Embodiment 1:

[0030] Please refer to the attached Figure 1-4 The shape of the dental wax itself is a rectangle, and a plurality of mesh positioning slits 2 are provided on the surface of the rectangular dental wax. When in use, we place the dried mesh on the mesh positioning slits 2 one by one using tweezers, as shown in the attached figure. Figure 5 As described above, by gently squeezing the two ends of the dental wax, a lot of carrier meshes can be fixed on the dyeing body 1 of the dental wax, as shown in the attached Figure 7-8 As described above, for the subsequent cleaning and staining, tweezers can be used to clamp the entire piece of dental wax and place it in a specified solution for staining and cleaning operations. This is convenient and quick, and can ensure the overall cleaning or staining time, control variables, facilitate subsequent average testing, and avoid errors.

[0031] Embodiment 2:

[0032] Please refer to the attached Figure 5-6 As mentioned above, because the immersion dyeing process takes about 20 minutes, we can place the dental wax with the carrier mesh in the dye solution without using tweezers to clamp the carrier mesh and move it. At this time, we need to prevent the dental wax from being placed in the solution. If the dental wax is tilted, will it affect the carrier mesh on it? After observation, it is found that only when the dental wax is tilted and the carrier mesh just touches the side wall of the solution, it may affect the carrier mesh. Therefore, if Figure 5 As described above, support columns 3 are provided at both ends of the dental wax, and the directions of the support columns 3 are as follows: Figure 5 The above-mentioned support column 3 is made of wood, which is consistent with the direction in which the grid is set, so as to avoid the inclination of the dental wax and the influence on the grid on the dental wax. The support column 3 is made of wood. Note that because the solution is a heavy metal salt, the material here must be non-metallic to avoid contaminating the solution.

[0033] The dyeing body 1 is provided with a spine 4 inside. Figure 5-6 As described above, when placing the carrier mesh on the dental wax dyeing body 1, it is necessary to press the two ends of the dental wax tightly by hand to further fix the carrier mesh. However, the material of the dental wax itself is relatively soft, so in order to prevent the carrier mesh from being squeezed during the squeezing process, the carrier mesh is pressed tightly on the two ends of the dental wax. Figure 5As described above, the tooth wax itself is too curved, and a spine 4 is placed inside the dyeing body 1. The material of the spine 4 can be metal because it is completely inside and will not come into contact with the solution. Of course, it can also be made of wood or other materials, and it mainly plays a certain supporting role.

Claims

1. A transmission electron microscope ultrathin section batch staining tool, characterized in that: It comprises a dyeing body (1), on which a plurality of web carrier positioning slits (2) are provided in accordance with the size of the web carrier, and the plurality of web carriers are respectively clamped on the web carrier positioning slits (2).

2. A transmission electron microscope ultrathin section batch staining tool according to claim 1, characterized in that: The material of the dyeing body (1) is a carrier mesh made of dental wax material. The positioning gap is squeezed by squeezing the dental wax, and then the carrier mesh is squeezed and fixed on the positioning gap.

3. A transmission electron microscope ultrathin section batch staining tool according to claim 2, characterized in that: Support columns (3) are provided at both ends of the tooth wax, and the material of the support columns (3) is a wooden support column (3).

4. A transmission electron microscope ultrathin section batch staining tool according to claim 3, characterized in that: A spine (4) is provided inside the dyeing body (1).

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