Centrifugal filtering device for donkey-hide gelatin peptide detection

Abstract

The utility model discloses a centrifugal filtering device for donkey-hide gelatin peptide detection, and relates to the technical field of solid-liquid separation. The top end of the barrel body is covered with a sealing cover, the bottom of the barrel body is provided with a collecting spherical shell in a threaded mode, a rotating motor is fixedly installed at the top end of the sealing cover, a rotating shaft is rotatably installed in the barrel body, an arc-shaped plate is fixedly installed on the rotating shaft, and a filtering box block is fixedly installed at the tail end of the arc-shaped plate; and a net bag is slidably mounted in the filter box block, so that collection and detection of centrifuged bottom-layer sediments can be completed, and the centrifugal detection efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of solid-liquid separation, and specifically relates to a centrifugal filter device for donkey-hide gelatin detection. Background Art

[0002] When testing and determining the content of donkey-hide gelatin peptides, a centrifugal filter device needs to be used multiple times. For example, the patent invention disclosure CN102277406A discloses a method for determining the content of donkey-hide gelatin oligopeptides, specifically:

[0003] 1. Method Summary:

[0004] Low molecular weight protein hydrolysates (including peptides and free amino acids) are soluble in trichloroacetic acid solution; high molecular weight proteins are easily precipitated in trichloroacetic acid solution. After the sample is dissolved in trichloroacetic acid solution, the precipitated protein material is separated by centrifugation, and the acid-soluble protein content in the centrifuged clear liquid is determined. The acid-soluble protein content in the clear liquid minus the free amino acid content is the content of oligopeptides.

[0005] 2. Analysis steps:

[0006] 2.1 Determination of acid-soluble protein content:

[0007] Weigh 2g (accurate to 1mg) of sample, add to a 10mL volumetric flask, dilute with 15% trichloroacetic acid solution, mix well, and let stand for 10min. Centrifuge the sample solution at 4000rpm for 10min, take all the clear liquid, and determine the acid-soluble protein in the clear liquid according to the method specified in GB / T 5009.5. The protein conversion factor is 6.25. The test result is converted to dry basis according to the drying loss of the sample;

[0008] 2.2 Determination of free amino acid content:

[0009] Sample pretreatment: Weigh 20-30 mg of sample, accurate to 0.0001 g, and dissolve evenly with 3% sulfosalicylic acid solution. Transfer the sample solution to a 50 ml volumetric flask and fix the volume. Centrifuge the sample solution on a centrifuge with a speed of 4000 r / min for 5 minutes to obtain a clear solution, then filter the clear solution with a 0.45 μm microporous filter membrane, transfer the filtrate to a 50 ml volumetric flask, fix the volume and use it as a sample for instrument detection. The rest of the operation is the same as the method specified in GB 12292 Determination of free amino acid content in fruit and vegetable juice.

[0010] Patent CN218853665U discloses a centrifugal filter device for detecting donkey-hide gelatin polypeptide, comprising a box body, a cover plate is provided on the top of the box body, a fixing mechanism is provided between the cover plate and the box body, a mounting groove is provided on the upper end of the box body, a water inlet pipe is fixedly connected to the upper end of the cover plate, the lower end of the water inlet pipe penetrates the lower end of the cover plate and extends into the mounting groove, the lower end of the cover plate is rotatably connected to a rotating tube, the lower side of the water inlet pipe is located in the rotating tube, the side wall of the rotating tube is fixedly connected to a rotating disk, and a driving motor is installed at the lower end of the box body. After centrifugal filtration, the bottom sediment after centrifugation is easily left inside the device and is difficult to clean. Each cleaning requires a lot of time and effort, resulting in low efficiency of centrifugal detection. Summary of the invention

[0011] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a centrifugal filter device for donkey-hide gelatin detection which can overcome the above problems or at least partially solve the above problems.

[0012] In order to solve the above technical problems, the basic concept of the technical solution adopted by the utility model is: a centrifugal filtration device for donkey-hide gelatin detection includes a cylinder, the top cover of the cylinder is equipped with a sealing cap, the bottom thread of the cylinder is equipped with a collecting spherical shell, the top of the sealing cap is fixedly installed with a rotating motor, a rotating shaft is rotatably installed in the cylinder, an arc plate is fixedly installed on the rotating shaft, a filter box block is fixedly installed at the tail end of the arc plate, and a net bag is slidably installed in the filter box block.

[0013] In order to enable the rotating motor to control the rotation of the rotating shaft, preferably, a limit block is fixedly installed on the driving end of the rotating motor, and a limit groove matching the limit block is provided on the rotating shaft.

[0014] In order to fix the net bag in the filter box block, preferably, a bottom clamping block is fixedly installed on the bottom end of the net bag, a top clamping block is fixedly installed on the top end of the net bag, and a rotating sealing block is rotatably installed on the top clamping block.

[0015] In order to improve the stability of the net bag in the filter box block, further, the bottom block is a magnet, and a magnetic block matching the bottom block is provided at the bottom end of the filter box block.

[0016] In order to prevent the donkey-hide gelatin liquid from entering the net bag again, preferably, a blocking sealing plate is fixedly installed at the rear end of the filter box block.

[0017] In order to prevent impurities from being adsorbed and condensed on the inner wall surface of the cylinder, further, a scraper is fixedly installed on the side end of the filter box block, and the scraper is against the inner wall surface of the cylinder.

[0018] After adopting the above technical scheme, the utility model has the following beneficial effects compared with the prior art: the utility model is a centrifugal filter device for detecting donkey-hide gelatin peptides, by placing the donkey-hide gelatin raw material into a net bag, and the net bag is placed in a filter box block, so that when the sample solution to be centrifuged is rotated at high speed, only the supernatant will separate from the filter box block and the net bag, while the bottom sediment after centrifugation will remain in the net bag, will not contact other equipment in the cylinder, and will not be adsorbed and solidified in the cylinder. Subsequently, only the net bag needs to be pulled out to complete the collection and detection of the bottom sediment after centrifugation, thereby improving the efficiency of centrifugal detection.

[0019] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In the attached picture:

[0021] Figure 1 It is a schematic diagram of the external overall structure of the utility model;

[0022] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0023] Figure 3 It is a schematic diagram of the arc plate structure of the utility model;

[0024] Figure 4 The utility model Figure 3 The structural diagram of A in the figure;

[0025] Figure 5 It is a schematic diagram of the structure of the net bag of the utility model.

[0026] In the figure: 1, cylinder; 2, sealing cover; 3, collecting ball shell; 4, rotating motor; 401, limit block; 5, rotating shaft; 6, arc plate; 7, filter box block; 701, blocking sealing plate; 702, scraper; 8, net bag; 801, bottom block; 802, top block; 803, rotating sealing block. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the embodiments of the utility model clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. The following embodiments are used to illustrate the utility model but are not used to limit the scope of the utility model. Example

[0028] Reference Figure 1-5As shown, the centrifugal filtration device for detecting donkey-hide gelatin comprises a cylinder 1, the top cover of the cylinder 1 is provided with a sealing cover 2, the bottom thread of the cylinder 1 is provided with a collecting spherical shell 3, a rotating motor 4 is fixedly installed on the top of the sealing cover 2, a rotating shaft 5 is rotatably installed in the cylinder 1, an arc plate 6 is fixedly installed on the rotating shaft 5, a filter box block 7 is fixedly installed at the tail end of the arc plate 6, and a net bag 8 is slidably installed in the filter box block 7.

[0029] A limit block 401 is fixedly installed on the driving end of the rotating motor 4, and a limit groove matching the limit block 401 is provided on the rotating shaft 5. When the cover 2 is removed, the limit block 401 is separated from the rotating shaft 5. After the cover 2 is installed, the limit block 401 is inserted into the limit groove at the top end of the rotating shaft 5, so that the rotating motor 4 can control the rotation of the rotating shaft 5.

[0030] A bottom clamping block 801 is fixedly installed at the bottom end of the net bag 8, a top clamping block 802 is fixedly installed at the top end of the net bag 8, a rotating sealing block 803 is rotatably installed on the top clamping block 802, the bottom clamping block 801 is a magnet, and a magnetic block matching the bottom clamping block 801 is provided at the bottom end of the filter box block 7. When the net bag 8 is placed in the filter box block 7, it is fixed to the bottom end of the filter box block 7 through the bottom clamping block 801, and the top clamping block 802 is fixed to the top end of the filter box block 7. The rotating sealing block 803 is rotated to open, and the sample solution to be centrifuged is placed in the net bag 8, and then the rotating sealing block 803 is rotated to close.

[0031] A blocking sealing plate 701 is fixedly installed at the rear end of the filter box block 7. Furthermore, a scraper 702 is fixedly installed at the side end of the filter box block 7. The scraper 702 is in contact with the inner wall surface of the cylinder 1. The blocking sealing plate 701 prevents the supernatant from entering the filter box block 7 and the net bag 8. The scraper 702 scrapes and washes the inner wall surface of the cylinder 1 to prevent the sediment from being adsorbed and solidified on the inner wall surface of the cylinder 1 and being difficult to clean.

[0032] The utility model removes the cover 2, puts the net bag 8 into the filter box block 7 from the top, fixes the bottom block 801 at the bottom end of the filter box block 7, and fixes the top block 802 at the top end of the filter box block 7, rotates to open the rotating seal block 803, puts the sample solution to be centrifuged into the net bag 8, and then rotates to close the rotating seal block 803, reinstalls the cover 2, inserts the limit block 401 into the limit groove at the top end of the rotating shaft 5, starts the rotating motor 4, drives the rotating shaft 5 to rotate, thereby drives the filter box block 7 to rotate at a high speed in the cylinder 1, so that the supernatant in the sample solution to be centrifuged is separated from the bottom sediment solid, and the supernatant falls into the collecting spherical shell 3. After the centrifugal rotation is completed, the collecting spherical shell 3 is threadedly removed, the supernatant in the collecting spherical shell 3 is collected and then tested, and the cover 2 is removed, the net bag 8 is drawn out from the filter box block 7, and the bottom sediment remaining in the net bag 8 after centrifugation is collected and tested.

[0033] The above description is only a preferred embodiment of the utility model, and does not constitute any form of limitation to the utility model. Although the utility model has been disclosed as a preferred embodiment as above, it is not used to limit the utility model. Any technician familiar with this patent can make equivalent replacements or changes based on the technical solution and utility model concept of the utility model without departing from the scope of the technical solution of the utility model, and all of them should be covered by the protection scope of the utility model.

Claims

1. A centrifugal filter device for donkey-hide gelatin detection, characterized in that: The invention comprises a cylinder (1), the top end cover of the cylinder (1) is provided with a sealing cover (2), the bottom of the cylinder (1) is threadedly provided with a collecting spherical shell (3), the top end of the sealing cover (2) is fixedly provided with a rotating motor (4), a rotating shaft (5) is rotatably provided in the cylinder (1), an arc plate (6) is fixedly provided on the rotating shaft (5), a filter box block (7) is fixedly provided at the rear end of the arc plate (6), and a net bag (8) is slidably provided in the filter box block (7).

2. The centrifugal filter device for detecting donkey-hide gelatin according to claim 1, characterized in that: A limit block (401) is fixedly mounted on the driving end of the rotating motor (4), and a limit groove matching the limit block (401) is provided on the rotating shaft (5).

3. The centrifugal filter device for detecting donkey-hide gelatin according to claim 1, characterized in that: A bottom clamping block (801) is fixedly mounted on the bottom end of the net bag (8), a top clamping block (802) is fixedly mounted on the top end of the net bag (8), and a rotating sealing block (803) is rotatably mounted on the top clamping block (802).

4. The centrifugal filter device for detecting donkey-hide gelatin according to claim 3, characterized in that: The bottom clamping block (801) is a magnet, and a magnetic block matching the bottom clamping block (801) is provided at the bottom end of the filter box block (7).

5. The centrifugal filter device for detecting donkey-hide gelatin according to claim 1, characterized in that: A blocking sealing plate (701) is fixedly mounted on the rear end of the filter box block (7).

6. The centrifugal filter device for detecting donkey-hide gelatin according to claim 5, characterized in that: A scraper (702) is fixedly mounted on the side end of the filter box block (7), and the scraper (702) abuts against the inner wall surface of the cylinder (1).

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