Rapid resuscitator for low-temperature sample

Abstract

The utility model discloses a quick resuscitator for a low-temperature sample, which comprises a water tank, a base, a shell and a keel, the shell covers the outer part of the keel, the keel is fixed with the base, the water tank is fixed in the shell, the water tank is fixed with the base, a horizontal displacement mechanism is arranged on the keel, a first-ladder vertical displacement mechanism and a second-ladder vertical displacement mechanism are arranged in the water tank, and the first-ladder vertical displacement mechanism and the second-ladder vertical displacement mechanism are arranged on the base. The second-ladder vertical displacement mechanism is arranged on the first-ladder vertical displacement mechanism, the first-ladder vertical displacement mechanism is fixedly connected with the horizontal displacement mechanism, a pressing plate and a bearing plate are arranged in the water tank, the pressing plate is installed on the second-ladder vertical displacement mechanism, the bearing plate is installed on the first-ladder vertical displacement mechanism, and a heating pipe is laid in the water tank. According to the rapid recovery device, the effect of rapidly recovering a sample to 37 DEG C can be achieved in a water bath heating and circulating activity mode, so that the survival rate of the sample is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of eyebrow pencils, in particular to a low-temperature sample rapid resuscitation device. Background Art

[0002] In the field of cell biology, an important way to preserve cell biological resources is liquid nitrogen freezing, that is, freezing cells in a liquid nitrogen tank at -130℃ to -196℃, and then taking out the frozen cells for revival when the experiment requires it. When reviving cells, the best operation is to quickly heat the cell cryopreservation tube just taken out of the liquid nitrogen tank to 37℃, so that it quickly passes through the -5~0℃ where cells are most vulnerable, to avoid ice crystals recrystallizing and causing damage to cells.

[0003] Chinese patent application number CN202222322739.7 discloses a biological sample water bath oscillating recovery device, including a sample loading mechanism, a lifting drive mechanism, an oscillating drive mechanism and a heating water tank mechanism; the sample loading mechanism is used to place and fix multiple cryopreservation tubes, and the sample loading mechanism can enter and exit the heating water tank mechanism and oscillate in the heating water tank mechanism under the control of the lifting drive mechanism and the oscillating drive mechanism. The utility model can perform oscillating recovery operations, realize batch cell recovery, improve the efficiency of cell recovery, and better fix the cryopreservation tubes to enhance the stability of cryopreservation tubes in liquid recovery.

[0004] The above patent adopts a water bath resuscitation method, but because the heating mechanism and the fixing mechanism are at a certain distance, and the temperature sensor is arranged near the sample, and the method adopted is a cyclic eccentric oscillation method, it is easy to cause the samples to still not be able to maintain the effect of synchronous resuscitation in the batch concentrated resuscitation link, and the water temperature near the resuscitation cannot actually be accurately maintained at around 37 degrees, which can easily lead to the problem of excessive sample loss rate.

[0005] Therefore, we propose a low-temperature sample rapid resuscitation device to solve the above problems. Utility Model Content

[0006] The utility model aims to solve the shortcomings in the prior art and proposes a low-temperature sample rapid resuscitation device.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] A low-temperature sample rapid resuscitation device comprises a water tank, a base, an outer shell, and a keel. The outer shell cover is arranged outside the keel, and the keel is fixed to the base. The water tank is fixed in the outer shell, and the water tank is fixed to the base. A horizontal displacement mechanism is installed on the keel. A first-ladder vertical displacement mechanism and a second-ladder vertical displacement mechanism are provided in the water tank. The second-ladder vertical displacement mechanism is arranged on the first-ladder vertical displacement mechanism. The first-ladder vertical displacement mechanism is fixedly connected to the horizontal displacement mechanism. A pressing plate and a receiving plate are provided in the water tank. The pressing plate is installed on the second-ladder vertical displacement mechanism, and the receiving plate is installed on the first-ladder vertical displacement mechanism. A heating pipe is laid in the water tank.

[0009] Preferably, a notch is provided at the upper end of the shell, and the notch is arranged directly above the water tank.

[0010] Preferably, the horizontal displacement mechanism includes a first electric cylinder, a first track frame, and a first movable block. The first track frame is fixed on the keel and arranged horizontally, the first electric cylinder is fixed on one side of the first track frame, and the first movable block is slidably mounted on the first track frame and fixedly connected to one end of the first electric cylinder.

[0011] Preferably, the travel distance of the first electric cylinder is not less than 40 centimeters.

[0012] Preferably, a slit is provided at the lower end of the first track frame, and the bottom of the first movable block is inserted into the slit.

[0013] Preferably, the vertical displacement mechanism of the ladder includes a second electric cylinder, a second track frame, and a second movable block. The second track frame is fixedly connected to the first movable block, the second electric cylinder is fixed to one end of the second track frame, the second track frame is arranged perpendicular to the first track frame, and the second movable block is slidably mounted on the second track frame and fixedly connected to one end of the second electric cylinder.

[0014] Preferably, the travel distance of the second electric cylinder is not less than 40 centimeters.

[0015] Preferably, the two-ladder vertical displacement mechanism includes a fixed frame and a third electric cylinder, one end of the fixed frame is fixedly connected to the second movable block, the other end of the fixed frame is fixedly connected to the receiving plate, the third electric cylinder is fixed on the fixed frame, and the end of the third electric cylinder is fixedly connected to the pressing plate.

[0016] Preferably, a limit plate is fixedly connected to the interior of the water tank.

[0017] Preferably, a plurality of liquid through holes are provided on the limiting plate, and the diameter of the liquid through holes is not less than 4 cm.

[0018] Preferably, a temperature measuring rod is provided in the water tank, and the temperature measuring rod is fixedly connected to the limiting plate.

[0019] Preferably, the temperature measuring rod is arranged near the receiving plate.

[0020] Preferably, the pressing plate is provided with a hole, and the diameter of the hole is no greater than 1 cm.

[0021] Preferably, the receiving plate is provided with a plurality of sockets, and sample tubes are inserted into the sockets.

[0022] Preferably, the pressing plate is arranged directly above the receiving plate.

[0023] Compared with the prior art, the beneficial effects of the utility model are:

[0024] By cooperating with the first electric cylinder, the second electric cylinder, the pressing plate and the receiving plate, the pressing plate and the receiving plate can be moved horizontally and vertically, and the water flow during water bath heating can be circulated and stirred, so that the concentrated resuscitation samples can be evenly contacted with the water flow, always maintain contact with the accurate 37-degree water flow, and quickly pass through the 0-5-degree easily damaged area, thereby improving the sample survival rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the external structure of a low-temperature sample rapid resuscitation device proposed by the utility model.

[0026] Figure 2 for Figure 1 Schematic diagram of the structure of the first-ladder vertical displacement mechanism and the second-ladder vertical displacement mechanism.

[0027] Figure 3 for Figure 1 Schematic diagram of the internal structure of the middle water tank.

[0028] In the figure: 1 water tank, 2 base, 3 shell, 4 keel, 5 pressing plate, 6 receiving plate, 7 heating tube, 8 notch, 9 first electric cylinder, 10 first track frame, 11 first movable block, 12 second electric cylinder, 13 second track frame, 14 second movable block, 15 fixed frame, 16 third electric cylinder, 17 limit plate, 18 liquid hole, 19 temperature measuring rod, 20 sample tube. Implementation

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Example

[0030] In the field of cell biology, an important way to preserve cell biological resources is liquid nitrogen freezing, that is, freezing cells in a liquid nitrogen tank at -130℃ to -196℃. When the experiment requires it, the frozen cells are taken out for revival. When reviving cells, the best operation is to quickly heat the cell cryopreservation tube just taken out of the liquid nitrogen tank to 37℃, so that it quickly passes through the -5~0℃ where cells are most vulnerable, to avoid ice crystals recrystallizing and causing damage to cells. Therefore, refer to Figure 1 This scheme designs a low-temperature sample rapid resuscitation device, including a water tank 1, a base 2, a shell 3, and a keel 4, wherein the keel 4 is used to support the main component structure, the base 2 is used for the overall load-bearing operation, and the shell 3 is used to protect the overall structure. The specific structure is: the shell 3 is covered on the outside of the keel 4, and the keel 4 is fixed to the base 2, the water tank 1 is fixed in the shell 3, and the water tank 1 is fixed to the base 2.

[0031] Reference Figure 2-3 In order to facilitate manual or automatic placement of the sample tube 20, a slot 8 is provided at the upper end of the housing 3, and the slot 8 is arranged directly above the water tank 1. A horizontal displacement mechanism is installed on the keel 4 for controlling the horizontal displacement operation of the sample tube 20.

[0032] Specifically: the horizontal displacement mechanism includes a first electric cylinder 9, a first track frame 10, and a first movable block 11. The first track frame 10 is fixed on the keel 4 and is horizontally arranged. The first electric cylinder 9 is fixed on one side of the first track frame 10. Under the control of the first electric cylinder 9, the first movable block 11 is freely moved in the horizontal direction on the first track frame 10, thereby adjusting the horizontal position of the sample tube 20.

[0033] Since the keel 4 is in a vertical state, in order to ensure stability during horizontal movement, the first movable block is slidably mounted on the first track frame 10. A slit is provided at the lower end of the first track frame 10. The bottom of the first movable block 11 is inserted into the slit and fixedly connected to one end of the first electric cylinder 9. The first movable block 11 maintains a good operating stability under the joint action of the first track frame 10 and the slit.

[0034] In order to ensure that the first electric cylinder 9 can always maintain contact with a sufficient area of ​​37-degree warm water during the water bath heating process, the travel distance of the first electric cylinder 9 is not less than 40 cm, so that during the heating process, it can be exposed to sufficient water flow through horizontal movement of sufficient length, thereby facilitating accurate control of the actual contact temperature.

[0035] See also Figure 3A ladder vertical displacement mechanism is provided in the water tank 1 for vertical control. The ladder vertical displacement mechanism is fixedly connected to the horizontal displacement mechanism to form a coordinated movement in the up, down, left and right directions to complete the sample tube 20 and water bath heating actions. Specifically: the ladder vertical displacement mechanism includes a second electric cylinder 12, a second track frame 13, and a second movable block 14. The second track frame 13 is fixedly connected to the first movable block 11. The second electric cylinder 12 is fixed to one end of the second track frame 13. The second track frame 13 is arranged vertically to the first track frame 10. The second movable block 14 is slidably sleeved on the second track frame 13 to slide freely, and is fixedly connected to one end of the second electric cylinder 12 for control. The second movable block 14 completes the movement under the control of the second electric cylinder 12, and is synchronously restricted by the movement of the first electric cylinder 9, thereby completing the movement in a superimposed manner.

[0036] A receiving plate 6 is provided in the water tank 1, and a plurality of sockets are provided on the receiving plate 6, and sample tubes 20 are inserted in the sockets. The receiving plate 6 is installed on a ladder vertical displacement mechanism. A heating tube 7 is laid in the water tank 1, and the water tank 1 is filled with water. The heating is achieved by the heating tube 7 and is kept constant at 37 degrees. A limiting plate 17 is fixedly connected inside the water tank 1 for limiting. A plurality of liquid holes 18 are opened on the limiting plate 17. The diameter of the liquid holes 18 is not less than 4 cm to facilitate the free flow of water. A temperature measuring rod 19 is provided in the water tank 1 for measuring the water temperature. The temperature measuring rod 19 is fixedly connected to the limiting plate 17 and remains fixed. The temperature measuring rod 19 is arranged near the receiving plate 6 for accurately measuring the actual water temperature near the sample tube 20.

[0037] During water bath heating, the placed sample tube 20 can maintain circular movement up, down, left and right in the water tank 1 under the joint control of the first electric cylinder 9 and the second electric cylinder 12, and the amplitude of movement is not less than 40 cm in any dimension, so that the sample tube 20 can always keep in contact with stable 37-degree water during water bath heating. The centralized processed sample tubes 20 can still evenly maintain the set water bath heating, achieving active cyclic heating, and the movement amplitude is large and the movement dimension is wide, which is more conducive to stable contact with the water body than the traditional eccentric small-range movement, thereby improving the sample survival rate. Example

[0038] Since in the first embodiment, frequent synchronous movements up, down, left and right are required, the sample tube 20 disposed on the receiving plate 6 is prone to shake or become loose and fall off during the following movement.

[0039] Therefore, refer to Figure 3A two-ladder vertical displacement mechanism is also provided in the water tank 1. The two-ladder vertical displacement mechanism is arranged on the one-ladder vertical displacement mechanism and moves with the one-ladder vertical displacement mechanism. The two-ladder vertical displacement mechanism includes a fixed frame 15 and a third electric cylinder 16. One end of the fixed frame 15 is fixedly connected to the second movable block 14, and the other end of the fixed frame 15 is fixedly connected to the receiving plate 6. A pressing plate 5 is provided in the water tank 1. The pressing plate 5 is arranged directly above the receiving plate 6. The third electric cylinder 16 is fixed on the fixed frame 15, and the end of the third electric cylinder 16 is fixedly connected to the pressing plate 5. A hole is opened on the pressing plate 5, and the diameter of the hole is not more than 1 cm. Therefore, in the process of multi-directional movement, the pressing plate 5 controlled by the third electric cylinder 16 presses multiple sample tubes 20, so that sufficient stability can always be maintained.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A low-temperature sample rapid resuscitation device, characterized in that: The utility model comprises a water tank (1), a base (2), an outer shell (3), and a keel (4), wherein the outer shell (3) is arranged outside the keel (4), and the keel (4) is fixed to the base (2), the water tank (1) is fixed in the outer shell (3), and the water tank (1) is fixed to the base (2), a horizontal displacement mechanism is installed on the keel (4), the water tank (1) is provided with a first-ladder vertical displacement mechanism and a second-ladder vertical displacement mechanism, the second-ladder vertical displacement mechanism is arranged on the first-ladder vertical displacement mechanism, the first-ladder vertical displacement mechanism is fixedly connected to the horizontal displacement mechanism, the water tank (1) is provided with a pressing plate (5) and a receiving plate (6), the pressing plate (5) is installed on the second-ladder vertical displacement mechanism, and the receiving plate (6) is installed on the first-ladder vertical displacement mechanism, and a heating pipe (7) is laid in the water tank (1).

2. A low-temperature sample rapid resuscitation device according to claim 1, characterized in that: A notch (8) is provided at the upper end of the housing (3), and the notch (8) is arranged directly above the water tank (1).

3. A low-temperature sample rapid resuscitation device according to claim 1, characterized in that: The horizontal displacement mechanism comprises a first electric cylinder (9), a first track frame (10), and a first movable block (11); the first track frame (10) is fixed on the keel (4) and arranged horizontally; the first electric cylinder (9) is fixed on one side of the first track frame (10); and the first movable block is slidably sleeved on the first track frame (10) and fixedly connected to one end of the first electric cylinder (9).

4. A low-temperature sample rapid resuscitation device according to claim 3, characterized in that: The travel distance of the first electric cylinder (9) is not less than 40 centimeters.

5. A low-temperature sample rapid resuscitation device according to claim 3, characterized in that: The lower end of the first track frame (10) is provided with a slit, and the bottom of the first movable block (11) is inserted into the slit.

6. A low-temperature sample rapid resuscitation device according to claim 3, characterized in that: The ladder vertical displacement mechanism comprises a second electric cylinder (12), a second track frame (13), and a second movable block (14); the second track frame (13) is fixedly connected to the first movable block (11); the second electric cylinder (12) is fixed to one end of the second track frame (13); the second track frame (13) is arranged perpendicular to the first track frame (10); the second movable block (14) is slidably sleeved on the second track frame (13) and fixedly connected to one end of the second electric cylinder (12).

7. A low-temperature sample rapid resuscitation device according to claim 6, characterized in that: The travel distance of the second electric cylinder (12) is not less than 40 centimeters.

8. A low-temperature sample rapid resuscitation device according to claim 6, characterized in that: The two-ladder vertical displacement mechanism comprises a fixed frame (15) and a third electric cylinder (16); one end of the fixed frame (15) is fixedly connected to the second movable block (14); the other end of the fixed frame (15) is fixedly connected to the receiving plate (6); the third electric cylinder (16) is fixed on the fixed frame (15); and the end of the third electric cylinder (16) is fixedly connected to the pressing plate (5).

9. A low-temperature sample rapid resuscitation device according to claim 1, characterized in that: A limiting plate (17) is fixedly connected to the interior of the water tank (1).

10. A low-temperature sample rapid resuscitation device according to claim 9, characterized in that: The limiting plate (17) is provided with a plurality of liquid passage holes (18), and the diameter of the liquid passage holes (18) is not less than 4 centimeters.

11. A low-temperature sample rapid resuscitation device according to claim 9, characterized in that: A temperature measuring rod (19) is provided in the water tank (1), and the temperature measuring rod (19) is fixedly connected to the limiting plate (17).

12. A low-temperature sample rapid resuscitation device according to claim 11, characterized in that: The temperature measuring rod (19) is arranged near the receiving plate (6).

13. A low-temperature sample rapid resuscitation device according to claim 1, characterized in that: The pressing plate (5) is provided with a hole, and the diameter of the hole is no greater than 1 centimeter.

14. A low-temperature sample rapid resuscitation device according to claim 1, characterized in that: The receiving plate (6) is provided with a plurality of insertion holes, and sample tubes (20) are inserted into the insertion holes.

15. The low-temperature sample rapid resuscitation device according to claim 1, characterized in that: The pressing plate (5) is arranged directly above the receiving plate (6).

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