Sample bin and sample bin detection platform

Abstract

The utility model discloses a sample bin and sample bin detection platform, wherein the sample bin comprises a fixed part and a sample bin body used for accommodating tissue samples, the sample bin body is arranged on the fixed part, the cross section of the sample bin body tends to decrease from top to bottom along the vertical direction, and the sample bin body is arranged on the fixed part. Light-transmitting structures are arranged on the two opposite sides of the sample bin body, and the sample bin is applied to the sample bin detection platform. The device has the advantage of being capable of detecting a thicker tissue sample.

Description

Technical Field

[0001] The utility model relates to the field of optical technology, in particular to a sample bin and a sample bin detection platform. Background Art

[0002] Light sheet or selective plane illumination microscopy is a type of fluorescence microscopy that examines tissue samples by placing them at the intersection of the illumination beam path and the detection beam path. The tissue sample is often placed in a sample chamber, which is then placed in a detection device for examination.

[0003] Most existing sample chambers have a flat bottom structure, and the thickness of the tissue samples that can be detected is very limited. In addition, the tissue samples need to be continuously moved by a translation stage to be accurately located at the intersection of the light beam path for detection. The tissue sample positioning process is time-consuming and inefficient, and this type of sample chamber is not suitable for slender tissues.

[0004] In view of this, the purpose of the utility model is to provide a new technical solution to solve the existing technical problems. Utility Model Content

[0005] In order to overcome the deficiencies of the prior art, the utility model provides a sample bin and a sample bin detection platform, which solves the problem that the thickness of tissue samples that can be detected by the prior sample bin is limited.

[0006] The technical solution adopted by the utility model to solve its technical problems is:

[0007] A sample bin comprises a fixing portion and a sample bin body for accommodating tissue samples. The sample bin body is arranged at the fixing portion. The cross section of the sample bin body decreases from top to bottom along the vertical direction. Opposite sides of the sample bin body are arranged as light-transmitting structures.

[0008] In the above structure, the sample chamber body is in a "V" shape.

[0009] In the above structure, the light-transmitting structure is light-transmitting thin glass.

[0010] In the above structure, the thickness of the light-transmitting thin glass is 0.05 mm-0.5 mm.

[0011] In the above structure, the fixing portion and the sample chamber body are integrally formed.

[0012] A sample chamber detection platform comprises a sample chamber with the above structure, and also comprises a liquid carrier, an illumination objective lens for emitting a light beam to illuminate a tissue sample, and an imaging objective lens for receiving detection light. The sample chamber can be raised and lowered on the liquid carrier, the illumination objective lens and the imaging objective lens are symmetrically arranged on opposite sides of the liquid carrier, and the illumination objective lens emits light toward the light-transmitting structure on one side, and the imaging objective lens receives light toward the light-transmitting structure on the other side.

[0013] In the above structure, a translation stage is further included. The translation stage is liftably arranged on the side of the liquid carrier stage facing away from the illumination objective lens and the imaging objective lens. The fixing part is detachably arranged on the translation stage.

[0014] In the above structure, the fixing portion is fixed to the translation stage by magnetic attraction.

[0015] The beneficial effects of the utility model are as follows: the sample bin is in a "V"-shaped structure, and can detect thicker tissue samples compared to a sample bin with a flat bottom structure; and the sample bin in the "V"-shaped structure is more conducive to the centering of the tissue sample, which is conducive to improving the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0017] Figure 1 This is a schematic diagram of the sample bin structure in the utility model;

[0018] Figure 2 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 3 This is a schematic diagram of the connection structure between the sample bin and the displacement stage in the utility model;

[0020] Figure 4 It is a schematic diagram of the cross-sectional structure of the utility model.

[0021] Figure numerals: 1. sample chamber; 11. sample chamber body; 111. light-transmitting structure; 12. fixing part; 121. magnet; 2. liquid carrier stage; 21. displacement stage; 3. illumination objective lens; 4. imaging objective lens. DETAILED DESCRIPTION

[0022] The following is combined with Figure 1-4 The utility model is further described.

[0023] The following will clearly and completely describe the concept, specific structure and technical effects of the utility model in combination with the embodiments and drawings, so as to fully understand the purpose, characteristics and effects of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the utility model. In addition, all the connection / connection relationships involved in the patent do not refer to the direct connection of components, but refer to the formation of a better connection structure by adding or reducing connection accessories according to the specific implementation situation. The various technical features in the creation of the utility model can be combined interchangeably without conflicting with each other.

[0024] Reference Figure 1 The utility model provides a sample bin 1, which includes a fixing portion 12 and a sample bin body 11, wherein the sample bin body 11 is used to accommodate tissue samples for testing of the tissue samples; the fixing portion 12 is used to install and connect the sample bin body 11 with other testing platforms. The fixing portion 12 is fixedly connected to opposite sides of the sample bin body 11, the sample bin body 11 is a structure with a cross section gradually decreasing from top to bottom along the vertical direction, and the side wall of the sample bin body 11 is set as a light-transmitting structure 111, and the light-transmitting structure 111 is used to transmit light for the testing platform to perform imaging testing on the tissue sample. Specifically, the side walls on opposite sides of the sample bin body 11 are set as the light-transmitting structure 111.

[0025] In this embodiment, the vertical section of the sample bin body 11 is a "V"-shaped structure, that is, the sample bin body 11 is an isosceles triangular prism structure with the bottom facing upward, and the two opposite rectangular sides of the isosceles triangular prism structure of the sample bin body 11 are set as a light-transmitting structure 111, and the light-transmitting structure 111 is a light-transmitting thin glass. Furthermore, the thickness of the light-transmitting glass is 0.05mm-0.5mm, and the light-transmitting glass can be installed on the sample bin body 11 by fixing methods such as bonding. The light-transmitting structure 111 is a very thin light-transmitting glass, which is conducive to reducing the light loss such as reflection and refraction during the detection of the detection platform, which is not only conducive to light transmission, but also conducive to clearer imaging of tissue samples. The sample bin body 11 and the fixing part 12 are integrally formed, which effectively ensures the reliability of the overall connection of the sample bin 1, and the integral molding setting is conducive to reducing the difficulty of processing.

[0026] Reference Figures 1 to 4The utility model also provides a sample bin detection platform, which is suitable for slender tissue samples, and includes a sample bin 1 of the structure described above, and the sample bin detection platform also includes a liquid carrier 2, an illumination objective lens 3 and an imaging objective lens 4. Among them, the illumination objective lens 3 is used to emit a light beam to illuminate the sample. The light path of the illumination light beam can be a straight line, or the direction of the light path can be changed by an optical device. In this embodiment, the illumination light beam is in the form of a light sheet. In addition, the wavelength of the illumination light beam emitted by the illumination objective lens 3 is set according to the actual needs of the tissue sample, so as to excite the tissue sample to excite fluorescence imaging. The imaging objective lens 4 is used to receive the detection light. After the laser light beam emitted from the illumination objective lens 3 excites the fluorescence of the tissue sample, the fluorescence light of the tissue sample or the reflected illumination light beam is received by the imaging objective lens 4.

[0027] Reference Figures 2 to 3 The sample chamber 1 can be raised and lowered on the liquid carrier 2. Specifically, a displacement table 21 can be raised and lowered on the liquid carrier 2. The displacement table 21 can be installed on the liquid carrier 2 by means of a cylinder, a linear slide rail or other structures. The sample chamber 1 can be detachably arranged on the displacement table 21. More specifically, a plurality of magnets 121 are evenly arranged on the fixing portion 12, and a plurality of magnets 121 are correspondingly arranged on the displacement table 21. The sample chamber 1 is positioned and fixed on the displacement table 21 by the magnets 121. The sample chamber 1 is detachably arranged on the displacement table 21 by means of magnetic attraction, which is convenient for disassembly and is conducive to the placement and replacement of tissue samples. In other embodiments, the sample chamber 1 and the displacement table 21 can also be connected and fixed by means of snap-fitting or other means that are easy to disassemble.

[0028] Reference Figure 2 and Figure 4 The illumination objective lens 3 and the imaging objective lens 4 are symmetrically arranged on the side of the liquid stage 2 facing away from the sample chamber 1, and the light emitting surface of the illumination objective lens 3 faces the light-transmitting structure 111 on one side of the sample chamber body 11 (at Figure 1 The light receiving surface of the imaging objective lens 4 faces the light-transmitting structure 111 on the other side of the sample chamber body 11. In this embodiment, the illumination light beam emitted by the illumination objective lens 3 and the light beam received by the imaging objective lens 4 are perpendicular to each other.

[0029] Since the sample bin body 11 is in a "V" shape, when the tissue sample is placed in the sample bin body 11, the narrow bottom of the sample bin body 11 positions the tissue, and there is no need to manually adjust the tissue sample to center it, which is conducive to improving work efficiency. In addition, the light-transmitting structure 111 surfaces on both sides of the sample bin body 11 can play a role in pressing the tissue sample, and there is no need to set up additional pressure blocks to fix the tissue sample, which effectively simplifies the detection structure. In addition, since the illumination objective lens 3 and the imaging objective lens 4 are also symmetrically arranged, the intersection of the optical paths of the illumination objective lens 3 and the imaging objective lens 4 and the tissue sample are located on the same vertical line, and the lifting and displacement stage 21 can scan and detect the sample in the thickness direction for imaging.

[0030] The implementation process of the utility model is:

[0031] During detection, the light beam emitted by the illumination objective lens 3 passes through the light-transmitting structure 111 and irradiates the tissue sample located in the sample chamber body 11. The fluorescence excited by the irradiation of the tissue sample is received by the imaging objective lens 4 via the light-transmitting structure 111 on the other side. An image of the tissue sample is formed on the imaging objective lens 4. By raising and lowering the displacement stage 21, scanning imaging in the thickness direction of the tissue sample is realized, which is beneficial for detecting relatively thick tissues.

[0032] The above is a specific description of the preferred implementation of the utility model, but the invention of the utility model is not limited to the described embodiments. Technical personnel familiar with the field can also make various equivalent deformations or substitutions without violating the spirit of the utility model. These equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A sample bin, characterized in that: It comprises a fixing part and a sample chamber body for accommodating tissue samples. The sample chamber body is arranged on the fixing part. The cross section of the sample chamber body decreases from top to bottom along the vertical direction. Two opposite sides of the sample chamber body are arranged as light-transmitting structures.

2. A sample chamber according to claim 1, characterized in that: The sample chamber body is in a "V"-shaped structure.

3. A sample chamber according to claim 1, characterized in that: The light-transmitting structure is light-transmitting thin glass.

4. A sample chamber according to claim 3, characterized in that: The thickness of the light-transmitting thin glass is 0.05 mm-0.5 mm.

5. The sample chamber according to claim 1, characterized in that: The fixing portion is integrally formed with the sample chamber body.

6. A sample chamber detection platform, characterized in that: The invention comprises the sample chamber as described in any one of claims 1 to 5, and further comprises a liquid carrier, an illumination objective lens for emitting a light beam to illuminate the tissue sample, and an imaging objective lens for receiving detection light, wherein the sample chamber can be raised and lowered on the liquid carrier, the illumination objective lens and the imaging objective lens are symmetrically arranged on opposite sides of the liquid carrier, and the illumination objective lens emits light toward one side of the light-transmitting structure, and the imaging objective lens receives light toward the other side of the light-transmitting structure.

7. The sample chamber detection platform according to claim 6, characterized in that: It also includes a displacement stage, which can be raised and lowered and arranged on the side of the liquid carrier stage facing away from the illumination objective lens and the imaging objective lens, and the fixing part can be detachably arranged on the displacement stage.

8. The sample chamber detection platform according to claim 7, characterized in that: The fixing portion is fixed to the translation stage by magnetic attraction.

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