Condensed cell sap bead forming device

Abstract

The utility model discloses a condensed cell sap bead forming device which comprises an open tubular outer shell and an inner shell, and the inner shell is arranged in an inner cavity of the outer shell in a sleeved mode. A liquid nitrogen pool is arranged in an inner cavity of the outer shell and located below the inner shell, and a top cover is arranged at an opening of the outer shell. The inner shell and the liquid nitrogen pool are respectively connected with a first liquid nitrogen pipe and a second liquid nitrogen pipe; the outer shell is concave inwards to form a drawer cavity, and the drawer cavity is located between the inner shell and the liquid nitrogen pool; the inner shell is provided with an upper cavity and a lower cavity, the upper cavity is provided with a liquid injection pipe and a hollow pipe arranged with the lower cavity, the liquid nitrogen pool is provided with a liquid nitrogen variable control valve, the drawer cavity is provided with a connecting pipe, and the liquid injection pipe, the hollow pipe, the connecting pipe, the liquid nitrogen variable control valve and the liquid nitrogen pool form pipeline conduction connection; and the liquid nitrogen variable control valve is electrically connected with a control system. According to the device, micron-sized cell liquid beads can be formed and can be directly stored in a liquid nitrogen tank during cryopreservation, programmed cooling is not needed, the cell cryopreservation time is greatly shortened, and cell damage caused by cryopreservation is prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of cell cryopreservation, in particular to a cell liquid bead cryopreservation device. Background Art

[0002] The current form of cell cryopreservation is mainly a method of gradually freezing a mixture of cells and cryopreservation solution from the outside to the inside. This method has high requirements for cryopreservation solution and may have excessive residues, which will cause great damage to the cells being revived and cultured. In contrast, micro-cryopreservation requires a lower content of cryopreservation solution, and the extremely rapid cooling method can avoid excessive cell damage. Utility Model Content

[0003] Based on the above problems, the problem to be solved by the present invention is to provide a cell liquid bead freezing device

[0004] The technical solution of the utility model is as follows:

[0005] A condensed cell droplet forming device comprises an outer shell and an inner shell, a liquid nitrogen pool, a liquid injection pipe, a first liquid nitrogen pipe, a second liquid nitrogen pipe, a liquid nitrogen variable control valve, a connecting pipe, a control system and a top cover; wherein:

[0006] The shell is a tubular structure with an open top, a horizontally concave drawer cavity is provided on the outer wall of the lower section of the shell, and a cryotube rack is provided in the drawer cavity; the top cover is adapted to cover the open end of the shell;

[0007] The inner shell is placed in the inner cavity of the outer shell and is located above the drawer cavity. The inner shell is a tubular structure with an open top. A hollow tube communicating with the drawer cavity is provided in the inner cavity of the inner shell. A partition is transversely provided in the inner cavity of the inner shell. The partition and the hollow tube divide the inner cavity of the inner shell into an upper chamber and a lower chamber.

[0008] The lower end of the first liquid nitrogen tube is connected to the lower chamber, and the upper end of the first liquid nitrogen tube is fixed on the top cover;

[0009] The liquid nitrogen pool is arranged at the bottom of the inner cavity of the shell and is located below the drawer cavity. The lower end of the second liquid nitrogen tube is connected to the liquid nitrogen pool, and the upper end of the second liquid nitrogen tube is fixed on the top cover.

[0010] The connecting tube is placed on the cryopreservation tube rack, and the two ends of the connecting tube are respectively connected to the hollow tube and the liquid nitrogen pool to form a sealed connection or a loose state;

[0011] The liquid injection pipe is fixedly arranged in the upper chamber of the inner shell, and a liquid path is formed between the liquid injection pipe, the hollow pipe, the connecting pipe and the liquid nitrogen pool;

[0012] The liquid nitrogen variable control valve is arranged on the pipeline between the liquid nitrogen pool and the connecting pipe, and is controlled by the control system, and is used to form cell liquid beads in the injection pipe in the liquid nitrogen pool and to draw the formed cell liquid beads back into the cryopreservation tube placed on the cryopreservation tube rack;

[0013] The control system is arranged on the top cover or the outer shell.

[0014] In one embodiment, in the condensed cell liquid bead forming device, the injection tube is fixed to the partition through a bracket; the number of the injection tubes is more than two, all the injection tubes are connected through a connecting drip nozzle, and the liquid outlet end of the connecting drip nozzle is connected to the hollow tube.

[0015] In one embodiment, in the condensed cell droplet forming device, a drip control valve is provided on each of the injection tubes.

[0016] In one embodiment, in the condensed cell liquid bead forming device, the device also includes a driving cylinder, an air pipe and a liquid pushing piston. The driving cylinder is arranged on the outer wall of the outer shell and is controlled by the control system. The liquid pushing piston can be arranged back and forth in the liquid injection container; the liquid pushing piston forms an air path connection with the driving cylinder through the air pipe; and an air path connection is formed between the liquid outlet end of the liquid injection container and the liquid injection pipe.

[0017] In one embodiment, in the condensed cell liquid bead forming device, a heat-insulating filling layer is provided in the upper chamber, and the material of the heat-insulating filling layer is polypropylene.

[0018] In one embodiment, in the condensed cell droplet forming device, a liquid nitrogen adsorption layer is provided in the lower chamber; the material of the liquid nitrogen adsorption layer is liquid nitrogen adsorption material.

[0019] In one embodiment, in the condensed cell droplet forming device, a liquid nitrogen control valve is provided in the second liquid nitrogen pipe, and the liquid nitrogen variable control valve is controlled by the control system.

[0020] In one embodiment, in the condensed cell liquid bead forming device, sealing covers are respectively provided at the upper ends of the first liquid nitrogen tube and the second liquid nitrogen tube.

[0021] The utility model provides a condensed cell liquid bead forming device. After cells pass through the device, they can form cell liquid beads with a particle size of 200-300 μm. When frozen, they can be directly placed in a liquid nitrogen tank for storage without programmed cooling, which greatly shortens the cell freezing time and prevents cell damage caused by freezing, which is beneficial to the cultivation of cells after cryopreservation and recovery. At the same time, cells are stored in the form of frozen liquid beads, the cell state is visualized, and whether there is freeze-thaw damage can be determined from the integrity of the cell liquid beads. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1A schematic diagram of the structure of the condensed cell liquid bead forming device for preparing cell liquid beads;

[0023] Figure 2 A schematic diagram of the structure of a condensed cell droplet forming device for recovering cell droplets;

[0024] Figure 3 A schematic diagram of the structure for cleaning the condensed cell liquid bead forming device;

[0025] Figure 4 for Figure 1 Sectional view of the AA part;

[0026] Figure 5 This is a schematic diagram of the top cover structure of the condensed cell liquid bead forming device;

[0027] Figure 6 Schematic diagram of the structure of the cryopreservation tube;

[0028] Figure 7 Schematic diagram of the structure of the cryopreservation tube rack;

[0029] Figure 8 This is a schematic diagram of the liquid nitrogen variable control valve structure. DETAILED DESCRIPTION

[0030] The preferred embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings.

[0031] like Figures 1 to 6 As shown, a condensed cell droplet forming device 100 provided by the utility model comprises an outer shell 1 and an inner shell 2, a liquid nitrogen pool 3, a liquid injection tube 4, a first liquid nitrogen tube 6, a second liquid nitrogen tube 7, a liquid nitrogen variable control valve 31, a connecting tube 8, a control system 14 and a top cover 13. The outer shell 1 is a tubular structure made of medical grade stainless steel, which can be a square tube, a round tube or an elliptical tubular structure. A round tubular structure is preferred in this embodiment. The top of the outer shell 1 is open and the bottom is closed, and the open end of the outer shell 1 is sealed and covered by the top cover 13. The diameter of the outer shell 1 is 40cm and the length is 100cm. A horizontally concave drawer cavity 102 is provided on the outer wall of the lower section of the outer shell 1 and 20cm from the bottom of the outer shell 1. The height of the drawer cavity 102 is 20m, the depth is about 30cm, and the width is 30cm.

[0032] like Figure 7As shown, a cryotube rack 9 is adapted to be provided in the drawer cavity 102, and the cryotube rack 9 includes a disc 901, a support leg 902, and a bottom plate 903; the disc 901 and the bottom plate 903 are fixedly connected by the support leg 903, and the longitudinal cross section forms an "I"-shaped structure; the bottom plate 903 plays a role in balancing the disc 901, and the disc 901 can rotate horizontally relative to the support leg 902. The disc 901 is provided with a plurality of through holes 911 for placing cryotubes 10 or connecting tubes 8 distributed along concentric circles with equal circumferential angles. The outer diameter of the cryotube rack 9 is slightly smaller than the depth and width of the drawer cavity, which is about 28 cm; the total height of the cryotube rack 9 after placing the cryotubes 10 or connecting tubes 8 is 18 cm.

[0033] The inner shell 2 is also a circular tubular structure made of medical grade stainless steel, with an open top and a closed bottom. The inner shell 1 has a diameter of 30 cm and a length of 50 cm. The inner shell 2 is placed in the inner cavity 101 of the outer shell 1 and is located above the drawer cavity 102. In the center of the inner cavity of the inner shell 2, a hollow tube 5 with a length of 30 cm and a diameter of 200-300 μm is vertically arranged. The lower end of the hollow tube 5 penetrates the bottom of the outer shell 2 and the upper wall 212 of the drawer cavity 102, and then forms a pipeline connection with the drawer cavity 102; the outer wall of the lower end of the hollow tube 5 forms a sealed connection with the bottom of the outer shell 2. A partition 211 is arranged transversely in the inner cavity of the inner shell 2, and the partition 211 is 20 cm away from the open end of the inner shell 2. A center hole 212 is arranged at the center of the partition 211. After the partition 211 is fixedly connected to the upper end of the hollow tube 5, the center hole 212 is connected to the hollow tube 5 pipeline. The partition plate 211 and the hollow tube 2 divide the inner cavity of the inner shell 2 into an upper chamber 201 and a lower chamber 202 .

[0034] A liquid nitrogen adsorption layer 11 is provided in the lower chamber 202 of the inner shell 2, and the material is a liquid nitrogen adsorption material, such as molecular sieve, activated carbon, porous polymer, etc. The liquid nitrogen adsorption layer 11 is filled and coated around the hollow tube 5. The lower end of the lower chamber 202 is connected to the lower end of the first liquid nitrogen tube 6 through a pipe mouth, and the upper end of the first liquid nitrogen tube 6 extends along the gap space between the inner shell 2 and the outer shell 1 to the open end of the outer shell 1 and is adapted to be fixed on the opening 132 of the top cover 13. When in use, a certain amount of liquid nitrogen is added to the lower chamber 202 of the inner shell 2 through the first liquid nitrogen tube 6, and is adsorbed by the liquid nitrogen adsorption layer 11. At this time, the function of the liquid nitrogen adsorption layer 11 is to ensure that the cell fluid flowing down the hollow tube 5 is rapidly programmed to cool down in the form of droplets, so as to achieve low-loss cryopreservation of cells.

[0035] The above-mentioned injection tube 4 is fixedly arranged in the upper chamber 201 of the inner shell 2 through a bracket 401. An injection container 20 is fixedly arranged on the injection tube 4. The volume of the injection container 20 is a circular tubular structure with a capacity of 5 to 50 ml. The injection container 20 has a certain hardness and can be a disposable injection container 20 made of PA material, or it can be made of medical unmodified material and can be reused. The lower end of the injection container 20 is a liquid outlet (not marked in the figure), which is sealed and fixedly connected to the injection tube 4. A pipeline conduction connection is formed between the injection container 20, the injection tube 4, and the hollow tube 5. The upper chamber 201 of the inner shell 2 is provided with a heat-insulating filling layer (not marked in the figure), which is made of polypropylene, and is used to ensure that the cell fluid 21 in the injection tube 4 and the injection container 20 maintains a relatively constant temperature. The upper end of the injection container 20 is exposed outside the top cover 13 through the fixing hole 131 provided on the top cover 13.

[0036] Preferably, in one embodiment, the number of the above-mentioned injection tubes 4 is three, and the liquid outlets between the three injection tubes 4 are connected by a connecting nozzle 402, and the connecting nozzle 402 is provided with three liquid inlets and one liquid outlet; the three liquid inlets of the connecting nozzle 402 are respectively connected to the sealed pipelines of the three injection tubes 4, and the liquid outlet of the connecting nozzle 402 is connected to the hollow tube 5 to form a pipeline conduction connection. In other embodiments, different numbers of injection tubes 4 and connecting nozzles 402 can also be provided as needed. The coordinated use of multiple injection containers 20, injection tubes 4 and connecting nozzles 402 can add cell fluid, cryopreservation protection liquid, cryopreservation wrapping liquid, etc. to different injection containers 20 as needed. Such coordinated use can protect the cells to be frozen and avoid freezing of the cells.

[0037] In another embodiment, in order to control the outflow of the cell solution from the injection tube 4, for example, the outflow of the cell solution 21 is 100 μl / min; a drip control valve 403 is provided on the pipeline between the injection tube 4 and the drip nozzle 402, and the drip control valve 403 is a solenoid valve controlled by the control system 14. The size of the cell solution beads 110 is prepared as needed, and the control system 14 controls the opening and closing amount of the drip control valve 403, thereby controlling the outflow of the cell solution 21.

[0038] The liquid nitrogen pool 3 is used to form cell liquid beads 110. The liquid nitrogen pool 3 is arranged at the bottom of the inner cavity 101 of the outer shell 1 and is located below the drawer cavity 102. The liquid nitrogen pool 3 is also a circular ring-shaped structure made of medical stainless steel. The lower end of the second liquid nitrogen pipe 7 is connected to the liquid nitrogen pool 3 pipeline, and the second liquid nitrogen pipe 7 extends along the gap space between the inner shell 2 and the outer shell 1 to the open end of the outer shell 1 and is adapted and fixed on the opening 132 of the top cover 13; the first liquid nitrogen pipe 6 and the second liquid nitrogen pipe 7 are arranged side by side; the upper ends of the first liquid nitrogen pipe 6 and the second liquid nitrogen pipe 7, that is, the injection ends are respectively provided with sealing covers 701 for sealing the first liquid nitrogen pipe 6 and the second liquid nitrogen pipe 7 to prevent liquid nitrogen from volatilizing or dust pollution. When in use, a certain amount of liquid nitrogen 301 is added to the liquid nitrogen pool 3 through the second liquid nitrogen pipe 7. A liquid receiving pipe 32 is provided on the upper wall of the liquid nitrogen pool 3 and directly opposite to the hollow tube 5 . The liquid receiving pipe 32 forms a pipeline sealing connection with the connecting pipe 8 or the freezing tube 10 in the drawer cavity 102 .

[0039] Preferably, in order to prevent the liquid nitrogen 301 in the liquid nitrogen pool 3 from volatilizing through the second liquid nitrogen pipe 7, a liquid nitrogen control valve 601 controlled by the control system 14 is provided in the pipeline of the second liquid nitrogen pipe 7. Under normal conditions, the liquid nitrogen control valve 601 is in a closed state; if the liquid nitrogen 301 is first added to the liquid nitrogen pool 3, the operator operates the control system 14 to control the liquid nitrogen control valve 601 to open, and then opens the cover 701 at the upper end of the second liquid nitrogen pipe 7 to add the liquid nitrogen 301 to the liquid nitrogen pool 3 through the second liquid nitrogen pipe 7; after that, the operator operates the control system 14 to control the liquid nitrogen control valve 601 to close.

[0040] The two ends of the connecting tube 8 are respectively set to form a pipeline conductive sealed connection or a loose state with the hollow tube 5 and the liquid nitrogen pool 3, so as to facilitate the formation and recovery of the cell liquid beads 110.

[0041] When preparing the cell liquid beads 110, the cell liquid 21 in the injection container 20 flows into the hollow tube 5 through the injection tube 4, and the cell liquid 21 is rapidly cooled through the hollow tube 5 to form a line-like cell liquid, and then drips into the surface of the liquid nitrogen 301 in the liquid nitrogen pool 3 through the connecting tube 8, the liquid receiving tube 32 and the liquid nitrogen variable control valve 31 placed in the drawer cavity 102, and forms a layer of cell liquid beads on the surface of the liquid nitrogen 301. The liquid nitrogen variable control valve 31 is arranged at the lower end of the liquid receiving tube 32, and the liquid nitrogen variable control valve 31 is arranged on the pipeline between the liquid nitrogen pool 3 and the cryopreservation tube 10, so as to form the cell liquid beads 110 in the liquid nitrogen pool 3 with the cell liquid 21 in the injection tube 4 and to draw the formed cell liquid beads back into the cryopreservation tube 10 placed on the cryopreservation tube rack 9.

[0042] The liquid nitrogen variable control valve 31 is an inverted "U"-shaped tubular structure, including a first branch pipe 301 and a second branch pipe 303; an electric control valve 303 is arranged on the first branch pipe 301, and a liquid pump 304 is arranged on the second branch pipe; a connection port 300 is arranged at the connection between the first branch pipe 301 and the second branch pipe 303, and the connection port 300 is connected to the liquid receiving pipe 32; the outer wall of the port of the first branch pipe 301 is sealedly connected to the liquid nitrogen pool 3 and then extends into the inner cavity of the liquid nitrogen pool 3 and is located above the liquid nitrogen 301, and the outer wall of the port of the second branch pipe 303 is also sealedly connected to the liquid nitrogen pool 3 and then extends into the liquid nitrogen 301 in the liquid nitrogen pool 3. The liquid nitrogen variable control valve 31 is controlled by the control system 14, mainly the electric control valve 303 and the liquid pump 304 are controlled by the control system 14; that is, the electric control valve 303 and the liquid pump 304 are opened or closed respectively under the action of the control system 14. When the cell fluid 21 in the connecting tube 8 flows downward through the liquid receiving tube 32, the electric control valve 303 in the liquid nitrogen variable control valve 31 is opened and the liquid pump 304 is closed, and the cell fluid 21 drips into the liquid nitrogen pool 3 through the first branch pipe 301, forming cell liquid beads on the surface of the liquid nitrogen 301; when the cell liquid beads in the liquid nitrogen pool 3 need to flow back to the cryopreservation tube 10 in the drawer cavity 102, the electric control valve 303 in the liquid nitrogen variable control valve 31 is closed and the liquid pump 304 is opened, and the liquid pump 304 draws the cell liquid beads 110 in the liquid nitrogen pool 3 back to the cryopreservation tube 10 through the second branch pipe 302; therefore, the length of the first branch pipe 301 should be less than the length of the second branch pipe 303, and the specific size depends on the depth of the liquid nitrogen pool 3.

[0043] like Figure 7 As shown, accordingly, in order to recover the cell droplets 110, the cryopreservation tube 10 is adaptively designed. The cryopreservation tube 10 is open at both ends, and an upper tube cover 10a and a lower tube cover 10b are respectively arranged at the two open ends, and a filter 10c is arranged at the lower end of the tube cavity of the cryopreservation tube 10 to prevent the leakage of the cell droplets. When it is necessary to recover the cell beads 110, the lower tube cover 10b of the cryopreservation tube 10 is opened, and the cryopreservation tube 10 is placed on the cryopreservation tube rack 9, and the disc 901 is rotated to make the lower port of the cryopreservation tube 10 form a pipeline conductive sealing connection with the liquid receiving pipe 32 of the liquid nitrogen pool 3; then, the control system 14 controls the liquid nitrogen variable control valve 31 to start, and the cell beads 110 on the surface of the liquid nitrogen 301 enter the cryopreservation tube 10 through the liquid nitrogen variable control valve 31 and the liquid receiving pipe 32, and the cryopreservation tube 10 filled with cell beads 110 and liquid nitrogen 301 is taken out from the drawer cavity 102, inverted and then left to stand, and the liquid nitrogen in the cryopreservation tube 10 is volatilized, leaving the cell beads 110; finally, inverted again, unscrewed the upper tube cover 10a, and transferred the cell beads 110 to a new conventional cryopreservation tube.

[0044] In one embodiment, preferably, in order to effectively fix the cryotube 10 or the connecting tube 8 placed on the cryotube rack 9 and avoid swinging during operation, which may cause cell liquid beads to form pipelines or recovery pipelines to deviate, a thermal insulation cotton clip 904 is also provided on the cryotube rack 9. The thermal insulation cotton clip 904 is fixed on the support leg 902 and is used to clamp and position the cryotube 10 or the connecting tube 8.

[0045] like Figures 1 to 3 As shown, the condensed cell liquid bead forming device 100 also includes a driving mechanism for driving the cell liquid to flow downward, and the driving mechanism includes a driving cylinder 12, an air pipe 121 and a liquid pushing piston 122; the driving cylinder 12 is fixedly arranged on the outer wall of the housing 1; the piston 122 is arranged on the liquid injection container 20, and the liquid pushing piston 122 can move up and down in the liquid injection container 20; the liquid pushing piston 122 forms an air path conductive connection with the driving cylinder 12 through the air pipe 121, and the liquid outlet end of the liquid injection container 20 forms an air path conductive connection with the liquid injection pipe 4. When in use, the operation control system 14 controls the driving cylinder 12 to start, and the high air pressure is pushed out through the air pipe 121 to drive the liquid pushing piston 122 to move toward the lower end of the liquid injection container 20, thereby driving the cell liquid 21 in the liquid injection container 20 to flow faster, providing a rapid formation of the cell liquid bead 110.

[0046] In order to facilitate the operator to intuitively observe the real-time working state of the condensed cell liquid bead forming device 100, a display screen 15 electrically connected to the control system 14 is provided on the top cover 13. All working condition information of the condensed cell liquid bead forming device 100 can be displayed through the display screen 15.

[0047] like Figure 3 As shown, after the above-mentioned condensed cell liquid beads 100 are prepared and recovered, the condensed cell liquid bead forming device 100 needs to be cleaned in preparation for storage for the next use. At this time, the cryopreservation tube rack 9 placed in the drawer cavity 102 is removed together with the cryopreservation tube and / or the connecting tube 8, and then the waste liquid collection dish 17 is adapted to be placed in the drawer cavity 102; at the same time, the washing liquid 23 is added to the liquid injection container 20. During cleaning, the operation control system 14 controls the driving cylinder 12 to start, and the high-pressure driving liquid pushing piston 122 is pushed out through the air pipe 121 to move toward the lower end of the liquid injection container 20, thereby driving the washing liquid 23 in the liquid injection container 20 to accelerate the flow, and quickly pass through the liquid injection tube 4 and the hollow tube 5 in turn, so that the washing liquid flows into the waste liquid collection dish 17; the cleaning operation is repeated many times.

[0048] In the present invention, the technical contents of the control system 14 in terms of electrical control of the above-mentioned drip control valve 403, electric control valve 303, liquid pump 304, driving cylinder 12 and display screen 15 are prior art and will not be described in detail here.

[0049] It should be understood that the above description of the preferred embodiments of the utility model is relatively detailed, and it cannot be regarded as a limitation on the scope of patent protection of the utility model. The scope of patent protection of the utility model shall be based on the attached claims.

Claims

1. A condensed cell liquid bead forming device, characterized in that: The device comprises an outer shell and an inner shell, a liquid nitrogen pool, a liquid injection pipe, a first liquid nitrogen pipe, a second liquid nitrogen pipe, a liquid nitrogen variable control valve, a connecting pipe, a control system and a top cover; wherein: The shell is a tubular structure with an open top, a horizontally concave drawer cavity is provided on the outer wall of the lower section of the shell, and a cryotube rack is provided in the drawer cavity; the top cover is adapted to cover the open end of the shell; The inner shell is placed in the inner cavity of the outer shell and is located above the drawer cavity. The inner shell is a tubular structure with an open top. A hollow tube communicating with the drawer cavity is provided in the inner cavity of the inner shell. A partition is transversely provided in the inner cavity of the inner shell. The partition and the hollow tube divide the inner cavity of the inner shell into an upper chamber and a lower chamber. The lower end of the first liquid nitrogen tube is connected to the lower chamber, and the upper end of the first liquid nitrogen tube is fixed on the top cover; The liquid nitrogen pool is arranged at the bottom of the inner cavity of the shell and is located below the drawer cavity. The lower end of the second liquid nitrogen tube is connected to the liquid nitrogen pool, and the upper end of the second liquid nitrogen tube is fixed on the top cover. The connecting tube is placed on the cryopreservation tube rack, and the two ends of the connecting tube are respectively connected to the hollow tube and the liquid nitrogen pool to form a sealed connection or a loose state; The liquid injection pipe is fixedly arranged in the upper chamber of the inner shell, and a liquid path is formed between the liquid injection pipe, the hollow pipe, the connecting pipe and the liquid nitrogen pool; The liquid nitrogen variable control valve is arranged on the pipeline between the liquid nitrogen pool and the connecting pipe, and is controlled by the control system, and is used to form cell liquid beads in the injection pipe in the liquid nitrogen pool and to draw the formed cell liquid beads back into the cryopreservation tube placed on the cryopreservation tube rack; The control system is arranged on the top cover or the outer shell.

2. The condensed cell liquid bead forming device according to claim 1, characterized in that: The injection tube is fixed on the partition through a bracket; the number of the injection tubes is more than two, all of the injection tubes are connected through a connecting drip nozzle, and the liquid outlet end of the connecting drip nozzle is connected to the hollow tube.

3. The condensed cell droplet forming device according to claim 2, characterized in that: A drip control valve controlled by the control system is arranged on each of the injection pipes.

4. The condensed cell liquid bead forming device according to claim 1, characterized in that: The device also includes a driving mechanism, which includes a driving cylinder, an air pipe and a liquid pushing piston. The driving cylinder is arranged on the outer wall of the shell and is controlled by the control system. The liquid pushing piston can be arranged in the liquid injection container in a reciprocating manner. The liquid pushing piston is connected to the driving cylinder through an air pipe to form an air path conduction connection; and an air path conduction connection is formed between the liquid outlet end of the liquid injection container and the liquid injection pipe.

5. The condensed cell liquid bead forming device according to claim 1, characterized in that: A heat-insulating filling layer is arranged in the upper chamber.

6. The condensed cell droplet forming device according to claim 1, characterized in that: A liquid nitrogen adsorption layer is arranged in the lower chamber.

7. The condensed cell droplet forming device according to claim 1, characterized in that: A liquid nitrogen control valve is provided in the second liquid nitrogen pipe, and the liquid nitrogen variable control valve is controlled by the control system.

8. The condensed cell droplet forming device according to claim 1, characterized in that: Sealing covers are respectively provided at the upper ends of the first liquid nitrogen tube and the second liquid nitrogen tube.

9. The condensed cell droplet forming device according to claim 1, characterized in that: The liquid nitrogen variable control valve is an inverted "U"-shaped tubular structure, including a first branch pipe and a second branch pipe; an electric control valve is arranged on the first branch pipe, and a liquid pump is arranged on the second branch pipe; a connecting port is arranged at the connection between the first branch pipe and the second branch pipe, and the connecting port is conductively connected with the connecting pipe; the electric control valve and the liquid pump are respectively controlled by the control system.

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