Hydrogel batch forming device for cell 3D culture

Abstract

The utility model relates to the technical field of forming equipment, and discloses a hydrogel batch forming device for cell 3D culture, which comprises a fixing frame and an adjusting rod, a rubber layer is fixedly sleeved in the fixing frame, the top of the rubber layer is provided with a forming cavity, a forming bulge is arranged in the forming cavity, and the adjusting rod is arranged in the forming cavity. The top of the fixing frame is movably sleeved with a sealing cover frame, a batch discharging assembly is arranged at the bottom of the fixing frame, and an adaptive hole is formed in the bottom face of the rubber layer. By opening the sealing cover frame and the turnover device, the batch discharging assembly faces upwards, and the bottom rod is pressed, so that the movable plate moves, the spring is stretched, the push rod moves along the adaptive hole, the rubber layer is pushed to deform, cured hydrogel is separated from the forming cavity, batch separation is completed, and during batch forming preparation, the production efficiency is improved. The hydrogel culture containers formed in batches are rapidly separated from the rubber layers, the separation efficiency is greatly improved, time is saved, the efficiency is improved, and the use effect is good.

Description

Technical Field

[0001] The utility model relates to the technical field of molding equipment, and more specifically, to a batch molding device of hydrogel for 3D cell culture. Background Art

[0002] Hydrogel is a type of extremely hydrophilic three-dimensional network structure gel. It swells rapidly in water and can hold a large volume of water in this swollen state without dissolving. Due to the existence of a cross-linked network, hydrogel can swell and hold a large amount of water. The amount of water absorbed is closely related to the degree of cross-linking. The higher the degree of cross-linking, the lower the water absorption. This property is very similar to a soft tissue. The water content in the hydrogel can be as low as a few percent or as high as 99%. The aggregation state of the gel is neither completely solid nor completely liquid. The behavior of a solid is that it can maintain a certain shape and volume under certain conditions, and the behavior of a liquid is that solutes can diffuse or penetrate from the hydrogel.

[0003] In the prior art, the batch molding device for cell 3D culture hydrogel, during use, for batch culture hydrogel molding, adopts the method of injecting liquid hydrogel into the culture mold to complete the molding preparation. As the preparation solidification is completed, the molded culture hydrogel is firmly sleeved in the molding cavity, making the actual detachment difficult. During batch molding, the detachment operation needs to be performed one by one, which greatly increases the difficulty of separation, and the batch separation efficiency is low, and the use effect for batch molding is not good. Utility Model Content

[0004] In order to overcome the deficiencies of the prior art, the utility model provides a batch molding device of hydrogel for cell 3D culture, which has the advantages of rapid batch demoulding and controlled molding accuracy.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a device for batch molding of hydrogel for cell 3D culture, comprising a fixed frame and an adjusting rod, a rubber layer is fixedly sleeved inside the fixed frame, a molding cavity is provided on the top of the rubber layer, a molding protrusion is provided inside the molding cavity, a sealing cover frame is movably sleeved on the top of the fixed frame, a batch discharge assembly is provided at the bottom of the fixed frame, an adaptation hole is provided on the bottom surface of the rubber layer, a storage cavity and a No. 1 crack are respectively provided inside the rubber layer, and the two ends of the No. 1 crack are respectively connected to the storage cavity and the molding cavity.

[0006] Preferably, a reserved plate is provided inside the molding cavity, the reserved plate is located on the top of the molding protrusion, and an adapting hole is opened at the bottom of the rubber layer, and the adapting hole corresponds to the molding cavity one by one.

[0007] Preferably, an internal cavity is provided inside the sealing cover frame, a heating tube is fixedly provided inside the sealing cover frame, the heating tube is located in the internal cavity, and a side plate is fixedly connected to a side of the sealing cover frame.

[0008] Preferably, a fixing rod is fixedly connected to the top of the fixing frame, the fixing rod is movably sleeved with the side plate, and a locking cap is threadedly sleeved on the outer surface of the fixing rod.

[0009] Preferably, the batch discharging assembly includes a bottom plate, a movable plate, a push rod, a spring, a bottom rod and a bottom opening, the bottom plate is fixedly sleeved on the bottom of the fixed frame, the movable plate is located above the bottom plate, the spring is fixedly connected between the movable plate and the bottom plate, the push rod is fixedly connected between the movable plates, the bottom rod is fixedly connected to the bottom of the movable plate, the bottom opening is opened at the bottom of the bottom plate, and the upper end of the push rod is movably sleeved in the adapter hole.

[0010] Preferably, a top cover is provided on the top of the rubber layer, the top cover is fixedly sleeved in the storage cavity, and a No. 2 crack is opened on the top surface of the top cover.

[0011] Preferably, a bottom cavity is opened on the bottom surface of the fixed frame, and a suction cup is fixedly connected to the bottom surface of the fixed frame, and the suction cup is communicated with the bottom cavity. The adjusting rod is threadedly sleeved on the top surface of the fixed frame and extends into the bottom cavity. A movable plug is movably sleeved inside the bottom cavity, and the top surface of the movable plug is fixedly connected to the adjusting rod.

[0012] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0013] 1. The utility model opens the sealing cover frame, flips the device, makes the batch discharge assembly face upward, presses the bottom rod, moves the movable plate, stretches the spring, and moves the push rod along the adapter hole to push the rubber layer to deform, so that the solidified hydrogel escapes from the molding cavity, and the batch separation is completed. When the batch molding preparation is realized, the batch-molded hydrogel culture container can be quickly separated from the rubber layer, which greatly improves the separation efficiency, saves time, improves efficiency, and has a good use effect.

[0014] 2. The utility model puts the liquid hydrogel to be formed into the top of the rubber layer, most of the liquid hydrogel is evenly distributed inside each molding cavity, a small amount remains on the top surface of the rubber layer, and a sealing cover frame is covered. When the amount of hydrogel entering the molding cavity is too much, when the sealing cover frame is pressed in, the inside of the molding cavity and the top of the rubber layer are hydraulically raised, so that the No. 1 crack and the No. 2 crack are opened, and the excess hydrogel is squeezed into the storage cavity. The elastic effect of the rubber layer maintains the self-sealing of the crack, and when there is excess hydrogel liquid, the crack is automatically opened by hydraulic pressure to complete the bypass storage of excess hydrogel, thereby ensuring the molding quality, reducing the trouble of cleaning the excess hydrogel, and avoiding the influence of the excess hydrogel on the molding quality, and the use effect is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of the utility model;

[0016] Figure 2 It is a cross-sectional schematic diagram of the utility model;

[0017] Figure 3 It is a top schematic diagram of the rubber layer of the utility model;

[0018] Figure 4 It is a cross-sectional schematic diagram of the rubber layer of the utility model;

[0019] Figure 5 for Figure 4 A schematic diagram of the structure enlargement at the center A;

[0020] Figure 6 It is an exploded schematic diagram of the batch discharging component of the utility model;

[0021] Figure 7 It is a partial cross-sectional view of the utility model.

[0022] In the figure: 1. fixed frame; 2. rubber layer; 3. molding cavity; 4. reserved plate; 5. sealing cover frame; 6. side plate; 7. fixed rod; 8. locking cap; 9. batch discharge assembly; 91. bottom plate; 92. movable plate; 93. push rod; 94. spring; 95. bottom rod; 96. bottom mouth; 10. adapter hole; 11. storage cavity; 12. crack No. 1; 13. bottom cavity; 14. adjusting rod; 15. movable plug; 16. suction cup; 17. top cover; 18. crack No. 2; 19. heating tube. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] like Figures 1 to 7 As shown, the utility model provides a batch molding device for hydrogel for cell 3D culture, comprising a fixed frame 1 and an adjusting rod 14, a rubber layer 2 is fixedly sleeved inside the fixed frame 1, a molding cavity 3 is provided on the top of the rubber layer 2, a molding protrusion is provided inside the molding cavity 3, a sealing cover frame 5 is movably sleeved on the top of the fixed frame 1, a batch discharge component 9 is provided at the bottom of the fixed frame 1, an adapting hole 10 is provided on the bottom surface of the rubber layer 2, a storage cavity 11 and a No. 1 crack 12 are respectively provided inside the rubber layer 2, and the two ends of the No. 1 crack 12 are respectively connected to the storage cavity 11 and the molding cavity 3.

[0025] A reserved plate 4 is provided inside the molding cavity 3 , and the reserved plate 4 is located on the top of the molding protrusion. An adapting hole 10 is opened at the bottom of the rubber layer 2 , and the adapting hole 10 corresponds to the molding cavity 3 one by one.

[0026] An internal cavity is provided inside the sealing cover frame 5 , a heating tube 19 is fixedly provided inside the sealing cover frame 5 , and the heating tube 19 is located in the internal cavity. A side plate 6 is fixedly connected to a side of the sealing cover frame 5 .

[0027] The top of the fixing frame 1 is fixedly connected with a fixing rod 7 , the fixing rod 7 is movably sleeved with the side plate 6 , and a locking cap 8 is threadedly sleeved on the outer surface of the fixing rod 7 .

[0028] Among them, the batch discharge component 9 includes a bottom plate 91, a movable plate 92, a push rod 93, a spring 94, a bottom rod 95 and a bottom opening 96. The bottom plate 91 is fixedly sleeved on the bottom of the fixed frame 1, the movable plate 92 is located above the bottom plate 91, the spring 94 is fixedly connected between the movable plate 92 and the bottom plate 91, the push rod 93 is fixedly connected between the movable plates 92, the bottom rod 95 is fixedly connected to the bottom of the movable plate 92, the bottom opening 96 is opened at the bottom of the bottom plate 91, and the upper end of the push rod 93 is movably sleeved in the adapter hole 10.

[0029] A top cover 17 is provided on the top of the rubber layer 2 . The top cover 17 is fixedly sleeved in the storage cavity 11 . A second crack 18 is formed on the top surface of the top cover 17 .

[0030] Among them, a bottom cavity 13 is opened on the bottom surface of the fixed frame 1, and a suction cup 16 is fixedly connected to the bottom surface of the fixed frame 1, and the suction cup 16 is communicated with the bottom cavity 13. The adjusting rod 14 is threadedly sleeved on the top surface of the fixed frame 1 and extends into the bottom cavity 13. A movable plug 15 is movably sleeved inside the bottom cavity 13, and the top surface of the movable plug 15 is fixedly connected to the adjusting rod 14.

[0031] The working principle and use process of the utility model are as follows: the liquid hydrogel to be formed is put into the top of the rubber layer 2, most of the liquid hydrogel is evenly distributed inside each molding cavity 3, a small amount remains on the top surface of the rubber layer 2, and the sealing cover frame 5 is covered. When the amount of hydrogel entering the molding cavity 3 is too much, when the sealing cover frame 5 is pressed in, the hydraulic pressure inside the molding cavity 3 and the top of the rubber layer 2 is increased, so that the No. 1 crack 12 and the No. 2 crack 18 are both opened, and the excess hydrogel is squeezed into the storage cavity 11, and the heating tube 19 in the sealing cover frame 5 is started, and the hydrogel is rapidly solidified; when the solidification molding is completed, the sealing cover frame 5 is opened, the device is flipped, so that the batch discharge component 9 faces upward, the bottom rod 95 is pressed, so that the movable plate 92 moves, the spring 94 is stretched, and the push rod 93 moves along the adapter hole 10, pushing the rubber layer 2 to deform, and the solidified hydrogel escapes from the molding cavity 3, completing the batch separation.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A batch forming device for hydrogels for 3D cell culture, comprising a fixing frame (1) and an adjusting rod (14), characterized in that: The fixing frame (1) is fixedly sleeved with a rubber layer (2) inside, a molding cavity (3) is provided on the top of the rubber layer (2), a molding protrusion is provided inside the molding cavity (3), a sealing cover frame (5) is movably sleeved on the top of the fixing frame (1), a batch discharge assembly (9) is provided at the bottom of the fixing frame (1), an adapting hole (10) is provided on the bottom surface of the rubber layer (2), a storage cavity (11) and a first crack (12) are respectively provided inside the rubber layer (2), and two ends of the first crack (12) are respectively connected to the storage cavity (11) and the molding cavity (3).

2. The device for batch forming hydrogels for 3D cell culture according to claim 1, characterized in that: A reserved plate (4) is provided inside the molding cavity (3), and the reserved plate (4) is located on the top of the molding protrusion. An adapting hole (10) is opened at the bottom of the rubber layer (2), and the adapting hole (10) corresponds one-to-one with the molding cavity (3).

3. The device for batch forming hydrogels for 3D cell culture according to claim 2, characterized in that: An internal cavity is provided inside the sealing cover frame (5), a heating tube (19) is fixedly provided inside the sealing cover frame (5), and the heating tube (19) is located in the internal cavity. A side plate (6) is fixedly connected to the side of the sealing cover frame (5).

4. The device for batch forming hydrogels for 3D cell culture according to claim 3, characterized in that: A fixing rod (7) is fixedly connected to the top of the fixing frame (1), the fixing rod (7) is movably sleeved with the side plate (6), and a locking cap (8) is threadedly sleeved on the outer surface of the fixing rod (7).

5. The device for batch forming hydrogels for 3D cell culture according to claim 4, characterized in that: The batch discharge assembly (9) comprises a bottom plate (91), a movable plate (92), a push rod (93), a spring (94), a bottom rod (95) and a bottom opening (96); the bottom plate (91) is fixedly sleeved on the bottom of the fixed frame (1); the movable plate (92) is located above the bottom plate (91); the spring (94) is fixedly connected between the movable plate (92) and the bottom plate (91); the push rod (93) is fixedly connected between the movable plates (92); the bottom rod (95) is fixedly connected to the bottom of the movable plate (92); the bottom opening (96) is opened at the bottom of the bottom plate (91); and the upper end of the push rod (93) is movably sleeved in the adapter hole (10).

6. The device for batch forming hydrogels for 3D cell culture according to claim 5, characterized in that: A top cover (17) is provided on the top of the rubber layer (2), and the top cover (17) is fixedly sleeved in the storage cavity (11), and a second crack (18) is provided on the top surface of the top cover (17).

7. The device for batch forming hydrogels for 3D cell culture according to claim 6, characterized in that: The bottom surface of the fixed frame (1) is provided with a bottom cavity (13), the bottom surface of the fixed frame (1) is fixedly connected with a suction cup (16), the suction cup (16) is communicated with the bottom cavity (13), the adjustment rod (14) is threadedly sleeved on the top surface of the fixed frame (1) and extends into the bottom cavity (13), the bottom cavity (13) is movably sleeved with a movable plug (15), the top surface of the movable plug (15) is fixedly connected with the adjustment rod (14).

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