Kit for detecting zearalenone

Abstract

The utility model relates to the technical field of biological detection, in particular to a kit for detecting zearalenone, which comprises a kit body, a detection test strip is arranged in the kit body, a kit cover is matched at the upper end of the kit body, and a sample adding hole and an observation hole are arranged on the kit cover. A water cavity is formed in the box body, and a water injection hole penetrating to the water cavity is formed in the upper end of the box body; an elastic hole plug is matched in the sample adding hole, and a dropper insertion opening is formed in the hole plug; the observation hole is matched with a transparent cover plate; the device is reasonable in design and simple in structure, a proper environment temperature can be created by injecting water with different temperatures through the arrangement of the water cavity, and the influence of the environment temperature on detection is reduced; through the design of the elastic hole plug and the transparent cover plate, the kit can be sealed, the detection test strip is prevented from being affected with damp, sample volatilization during detection is avoided, and the use effect of the kit and the detection safety are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a kit for detecting zearalenone. Background Art

[0002] Zearalenone is mainly produced by Fusarium graminearum. Pink Fusarium, Fusarium moniliformis, Fusarium trilineum and other Fusarium can also produce this toxin. Li Jilun found in 1980 that zearalenone is also present in many crops such as wheat and soybeans. Zearalenone mainly contaminates grains such as corn, wheat, rice, barley, millet and oats. Among them, the positive detection rate of corn is 45%, and the highest toxic content can reach 2909 mg / kg; the detection rate of wheat is 20%, and the toxic content is 0.364-11.05 mg / kg. Zearalenone has strong heat resistance and is completely destroyed only after being treated at 110°C for 1 hour. Zearalenone has estrogen-like effects, which can cause acute and chronic poisoning in animals, abnormal reproductive function or even death of animals, and can cause huge economic losses to livestock farms. Therefore, it is very necessary to detect zearalenone.

[0003] The kit used in the prior art has the following deficiencies during use:

[0004] 1. The detection of zearalenone should be carried out at an ambient temperature of 22-27°C, which is generally easy to achieve in spring and autumn. The kit used in the prior art does not have the function of adjusting the ambient temperature. The use of the kit in summer and winter will affect the use effect of the kit.

[0005] 2. The sample addition hole and observation hole of the kit used in the prior art are both in an open state. The zearalenone test strip in the kit in an open state is very susceptible to moisture, and the zearalenone sample extract is a volatile liquid, so the use effect and safety of the kit are poor. Utility Model Content

[0006] In view of the above-mentioned shortcomings of the prior art, the purpose of the present utility model is to provide a kit for detecting zearalenone, which is used to solve the problems that the kit used in the prior art does not have the function of adjusting the ambient temperature and the kit does not have a sealing function.

[0007] In order to achieve the above-mentioned purpose and other related purposes, the utility model provides a kit for detecting zearalenone, comprising a box body, a test strip is arranged in the box body, a box cover is matched at the upper end of the box body, and a sample addition hole and an observation hole are opened on the box cover;

[0008] The box body is provided with a water cavity, the upper end of the box body is provided with a water injection hole penetrating to the water cavity, and the water injection hole is matched with a detachable hole cover;

[0009] The sample adding hole is equipped with an elastic hole plug, and the hole plug is provided with a dropper insertion port;

[0010] The observation hole is matched with a transparent cover plate.

[0011] In an embodiment of the present invention, at least two water injection holes are provided, and the plurality of water injection holes are distributed at the upper end of the same side of the box body.

[0012] In an embodiment of the present invention, the hole plug is made of rubber material.

[0013] In one embodiment of the present invention, the dropper insertion opening is configured as a tapered opening structure that is narrow at the top and wide at the bottom.

[0014] In one embodiment of the present invention, a partition is provided at the lower end of the test strip.

[0015] In one embodiment of the utility model, the test strip comprises a substrate and a sample pad, a binding pad, a nitrocellulose membrane, and a water-absorbing pad bonded to the substrate and overlapped in sequence;

[0016] The nitrocellulose membrane is provided with a T line and a C line, wherein the T line is arranged close to the binding pad side, and the C line is arranged close to the water absorption pad side;

[0017] The sample addition hole is located above the sample pad, and the observation hole is located above the T-line and C-line areas of the nitrocellulose membrane.

[0018] In one embodiment of the present invention, the conjugate pad is provided with a zearalenone antibody labeled with fluorescent microspheres, the zearalenone antigen is embedded in the T line, and the goat anti-rabbit secondary antibody is embedded in the C line.

[0019] As described above, the kit for detecting zearalenone of the utility model has the following beneficial effects:

[0020] 1. By setting a water cavity and a water injection hole on the box body, when the test kit is used to detect zearalenone in summer or winter, the test kit can be tested at an ambient temperature within a specific range by injecting warm water or cold water, thereby reducing usage restrictions and improving usage effects;

[0021] 2. By providing an elastic hole plug and a transparent cover plate, the elastic hole plug can cooperate to close the sample addition hole, and because the elastic hole plug has shrinkage elasticity, the dropper insertion port provided on the elastic hole plug is closed in a free state, which can prevent water vapor from invading the reagent kit from the sample addition hole before sample addition or the sample from volatilizing from the sample addition hole after sample addition without affecting the drop addition of the sample; the transparent cover plate can close the observation hole without affecting the observation effect of the CT line display sample in the reagent kit, which can solve the problem that the zearalenone test strip is extremely susceptible to moisture and the problem that the sample extract containing zearalenone is easy to volatilize, further improve the use effect of the reagent kit, and improve the safety of the use of the reagent kit;

[0022] 3. The kit for detecting zearalenone provided by the utility model has a reasonable design and a simple structure. The water chamber can create a suitable reagent detection environment temperature by injecting water of different temperatures, thereby reducing the influence of the ambient temperature on the detection. The design of the elastic hole plug and the transparent cover can seal the kit to prevent the test strip from getting damp and the sample from volatilizing during the detection, thereby improving the use effect of the kit and the safety of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Shown is a schematic diagram of the main structure of the utility model.

[0024] Figure 2 Shown is a schematic cross-sectional view of the structure of the utility model.

[0025] Component number description

[0026] Box body 1; water cavity 11; water injection hole 12; box cover 2; sample addition hole 21; observation hole 22; substrate 3; sample pad 31; binding pad 32; nitrocellulose membrane 33; T line 331; C line 332; water absorption pad 34; hole plug 4; dropper insertion port 41; transparent cover plate 5; partition plate 6; hole cover 7. DETAILED DESCRIPTION

[0027] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0028] See also Figure 1 to Figure 2It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the utility model, so they have no substantial technical significance. Any modification of the structure, change in the proportion relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the utility model without affecting the effects and purposes that can be achieved by the utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the utility model. The change or adjustment of their relative relationship should also be regarded as the scope of the implementation of the utility model without substantially changing the technical content.

[0029] See also Figure 1-2 The utility model provides a kit for detecting zearalenone, comprising a box body 1, a test strip is arranged in the box body 1, a box cover 2 is matched at the upper end of the box body 1, and a sample loading hole 21 and an observation hole 22 are opened on the box cover 2; the test strip comprises a substrate 3 and a sample pad 31, a conjugation pad 32, a nitrocellulose membrane 33, and a water absorption pad 34 which are bonded to the substrate 3 and overlapped in sequence; a T line 331 and a C line 332 are arranged on the nitrocellulose membrane 33, the T line 331 is arranged close to the conjugation pad 32, and the C line 332 is arranged close to the water absorption pad 34; the sample loading hole 21 is located above the sample pad 31, and the observation hole 22 is located above the area where the T line 331 and the C line 332 are arranged on the nitrocellulose membrane 33. The conjugation pad 32 is provided with a zearalenone antibody labeled with a fluorescent microsphere, the T line 331 is embedded with a zearalenone antigen, and the C line 332 is embedded with a goat anti-rabbit secondary antibody. When used specifically, the sample is dripped onto the test paper strip through the sample addition hole 21, and the zearalenone in the sample solution to be tested is combined with the zearalenone antibody labeled with the fluorescent microspheres in the conjugate pad 32 and chromatographed forward by capillary action. After reaching the detection area, the zearalenone antibody antigen fixed on the T line 331 is combined with the remaining unbound zearalenone antibody labeled with the fluorescent microspheres; the concentration of the yellow zearalenone antibody labeled with the fluorescent microspheres bound on the T line 331 is inversely proportional to the concentration of zearalenone in the sample, and the concentration of the fluorescent marker bound to the C line 332 is independent of the concentration of zearalenone in the sample.

[0030] The box body 1 is provided with a water cavity 11, and the upper end of the box body 1 is provided with a water injection hole 12 that passes through the water cavity 11. There are at least two water injection holes 12, and the multiple water injection holes 12 are distributed at the upper end of the same side of the box body 1; the water injection holes 12 are equipped with a detachable hole cover 7; by arranging the water cavity 11 and the water injection holes 12 on the box body 1, when the test kit is used to detect zearalenone in summer or winter, the test kit can be tested at an ambient temperature within a specific range by injecting warm water or cold water, thereby reducing usage restrictions and improving usage effects.

[0031] The sample adding hole 21 is equipped with an elastic hole plug 4, specifically, the hole plug 4 is made of rubber material; the hole plug 4 is provided with a dropper insertion port 41 with a tapered mouth structure that is narrow at the top and wide at the bottom; the rubber hole plug 4 can cooperate to close the sample adding hole 21, and because the elastic hole plug 4 has shrinkage elasticity, the dropper insertion port 41 opened on the elastic hole plug 4 is closed in a free state, which can prevent water vapor from invading the reagent kit from the sample adding hole 21 before sample addition or the sample from volatilizing from the sample adding hole 21 after sample addition without affecting the drop addition of the sample; the observation hole 22 is equipped with a transparent cover plate 5, which can close the observation hole 22 without affecting the observation effect of the CT line display in the reagent kit, and can solve the problem that the zearalenone test strip is extremely susceptible to moisture and the problem that the sample extract containing zearalenone is easy to volatilize. The elastic hole plug 4 and the transparent cover plate 5 can be used in combination to further improve the use effect of the reagent kit and improve the safety of the use of the reagent kit.

[0032] A partition 6 is provided at the lower end of the test paper strip. In actual use, due to the problem of heat loss, the water injection temperature is often slightly higher than the required ambient temperature. The partition 6 can prevent the test paper strip from directly contacting the water temperature and avoid the influence of overheating.

[0033] In summary, the kit for detecting zearalenone provided by the utility model has a reasonable design and a simple structure. The water chamber 11 is set to create a suitable reagent detection environment temperature by injecting water of different temperatures, thereby reducing the influence of the environment temperature on the detection. The design of the elastic hole plug 4 and the transparent cover plate 5 can seal the kit, prevent the test strip from getting damp, and prevent the sample from volatilizing during the detection, thereby improving the use effect of the kit and the safety of the detection. Therefore, the utility model effectively overcomes various shortcomings in the prior art and has a high industrial utilization value.

[0034] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed in the present invention shall still be covered by the claims of the present invention.

Claims

1. A kit for detecting zearalenone, comprising a box body (1), a test strip is arranged in the box body (1), a box cover (2) is matched at the upper end of the box body (1), and a sample addition hole (21) and an observation hole (22) are opened on the box cover (2); It is characterized in that The box body (1) is provided with a water cavity (11), the upper end of the box body (1) is provided with a water injection hole (12) penetrating into the water cavity (11), and a detachable hole cover (7) is provided in the water injection hole (12); The sample adding hole (21) is matched with an elastic hole plug (4), and the hole plug (4) is provided with a dropper insertion port (41); The observation hole (22) is matched with a transparent cover plate (5).

2. The kit for detecting zearalenone according to claim 1, characterized in that: At least two water injection holes (12) are provided, and the plurality of water injection holes (12) are distributed at the upper end of the same side of the box body (1).

3. The kit for detecting zearalenone according to claim 1, characterized in that: The hole plug (4) is made of rubber material.

4. The kit for detecting zearalenone according to claim 3, characterized in that: The dropper insertion opening (41) is configured as a tapered opening structure that is narrow at the top and wide at the bottom.

5. The kit for detecting zearalenone according to claim 1, characterized in that: A partition (6) is provided at the lower end of the test strip.

6. The kit for detecting zearalenone according to claim 1, characterized in that: The test paper strip comprises a substrate (3) and a sample pad (31), a binding pad (32), a nitrocellulose membrane (33), and a water-absorbing pad (34) which are bonded to the substrate (3) and overlapped in sequence; The nitrocellulose membrane (33) is provided with a T line (331) and a C line (332), wherein the T line (331) is arranged close to the binding pad (32), and the C line (332) is arranged close to the water absorption pad (34); The sample addition hole (21) is located above the sample pad (31), and the observation hole (22) is located above the area where the T line (331) and the C line (332) are set on the nitrocellulose membrane (33).

7. The kit for detecting zearalenone according to claim 6, characterized in that: The conjugate pad (32) is provided with a zearalenone antibody labeled with fluorescent microspheres, the T line (331) is embedded with a zearalenone antigen, and the C line (332) is embedded with a goat anti-rabbit secondary antibody.

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