Mother strain mycelium culture tube

Abstract

The utility model provides a mother strain hypha culture tube, which relates to the technical field of strain culture devices, and comprises a culture tank, a water vapor collecting tube and a top plug, the upper section and the lower section of the culture tank are respectively a tank-shaped evaporation part and a tubular culture part, the culture part is communicated with the evaporation part, and the bottom of the culture part is sealed; the culture groove is placed in the water vapor collecting pipe and is matched with the inner diameter width of the water vapor collecting pipe, a spaced backflow area is formed between the top wall of the culture part and the top wall of the water vapor collecting pipe, the bottom end of the water vapor collecting pipe is closed, and the lower portion of the outer wall of the water vapor collecting pipe is provided with an inclined supporting piece; the mother strain hypha culture device can solve the problems that a traditional test tube is not prone to being obliquely placed and prone to toppling and rotating when used for culture in the existing mother strain hypha culture process, water drops evaporated to the wall of the test tube can submerge hyphae, and the culture test tube is abandoned when the whole culture medium is infected after infectious microbes exist in the mother strain hypha culture process.

Description

Technical Field

[0001] The utility model relates to the technical field of bacterial strain culture devices, in particular to a mother strain mycelium culture tube. Background Art

[0002] In the cultivation of edible fungi, the number of mother strains obtained by the strain separation method is limited and it is difficult to meet the production needs. Therefore, after the mother strain mycelium is cultured in a test tube, it is transplanted to a new slant culture medium for cultivation and growth, which can expand the reproduction. The cultivation process of the mother strain mycelium is as follows: according to the needs of the strain, a suitable culture medium is selected to make a mixed liquid, a clean test tube is taken and the mixed liquid is dripped into the test tube with a dropper and plugged tightly with a cotton ball, and then the test tube filled with the liquid is placed in a sterilization box for high-temperature sterilization, the tube is tilted to make the liquid in the test tube into an inclined surface, the strain stored in the bottle is taken out, a small amount is taken into the culture medium in the test tube with a sterile pipette, and then the sealing plug is refilled, and after completion, it is placed in an incubator for cultivation, and the mother strain mycelium is obtained after the cultivation time is 2 to 3 weeks under suitable temperature and humidity conditions.

[0003] At present, the mother strain mycelium culture process uses traditional test tubes for culture, and there are the following problems in the culture: First, in order to obtain a larger area and facilitate operation, in order to make the water vapor generated during the culture process flow back to the bottom and reduce the impact on the upper mycelium, the test tube needs to be tilted for mother strain mycelium culture, but traditional test tubes are not easy to be tilted and are prone to tilting and rotating; second, the water droplets that evaporate to the wall of the test tube and flow back will flood the mycelium. If the residual bacteria on the wall of the test tube encounter the water droplets flowing back, it will cause the entire culture medium to be infected, making the culture tube invalid. Therefore, this application proposes a mother strain mycelium culture tube. Utility Model Content

[0004] In order to overcome the problems in the background technology, the utility model provides a mother culture mycelium culture tube, which solves the problems that the current mother culture mycelium culture process using traditional test tubes is difficult to tilt and place, prone to tipping and rotation, and water droplets that evaporate and flow back to the test tube wall will flood the mycelium. If miscellaneous bacteria are encountered, the miscellaneous bacteria will infect the entire culture medium and make the culture tube invalid.

[0005] A mother culture mycelium culture tube comprises a culture tank, a water vapor collection tube and a top plug, wherein the upper section and the lower section of the culture tank are respectively a tank-shaped evaporation part and a tubular culture part, the culture part is connected with the evaporation part and the bottom is sealed, the culture tank is placed in the water vapor collection tube and matches the inner diameter width of the water vapor collection tube, a reflux zone is spaced between the top wall of the culture part and the top wall of the steam collection tube, the bottom end of the water vapor collection tube is closed, an oblique support sheet is provided at the lower part of the outer wall of the water vapor collection tube, and the top plug is installed in the top opening of the water vapor collection tube and contacts with the top of the culture tank.

[0006] Furthermore, a liquid collection area is provided between the bottom of the water vapor collection tube and the bottom of the culture tank, and the liquid collection area is filled with filter cotton.

[0007] Furthermore, the bottom of the water vapor collection pipe is open and sealed by a bottom plug.

[0008] Furthermore, rubber limiting strips are arranged on both sides of the water vapor collection tube to interfere with the culture tank.

[0009] Compared with the prior art, the beneficial effects of the utility model are:

[0010] The present application is provided with a culture trough and a water vapor collection tube. The culture trough is installed in the water vapor collection tube. The lower part of the outer wall of the water vapor collection tube is provided with an inclined support piece to support the culture trough to be placed at an angle, so as to avoid the problem that the traditional test tube culture is easily tilted and rotated when placed at an angle. The culture trough of the present application includes a trough-shaped evaporation part and a tubular culture part. The culture part can add culture medium to culture mycelium. The evaporation part can make the water vapor generated during the culture process evaporate to the top of the water vapor collection tube and flow back to the bottom of the water vapor collection tube to be separated from the culture medium, which solves the problem that the water droplets evaporated to the test tube wall and flowed back to the mycelium in the existing test tube culture, and if miscellaneous bacteria are encountered, the miscellaneous bacteria will infect the entire culture medium and make the culture tube invalid. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to clearly illustrate the technical solutions in the embodiments of the present utility model, the drawings required for use in describing the embodiments are described.

[0012] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0013] Figure 2 It is a schematic diagram of the internal structure of the utility model;

[0014] Figure 3 This is a schematic diagram of the culture tank structure of the utility model.

[0015] 1-culture tank, 11-culture part, 12-evaporation part, 2-water vapor collection tube, 21-inclined support plate, 22-limiting strip, 3-top plug, 4-reflux area, 5-liquid collection area, 6-filter cotton, 7-bottom plug. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and beneficial effects of the present invention clearer, the preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings to facilitate understanding by technicians.

[0017] The utility model discloses a mother seed mycelium culture tube, referring to Figure 1-3A mother culture mycelium culture tube comprises a culture tank 1, a water vapor collection tube 2 and a top plug 3, wherein the upper section and the lower section of the culture tank 1 are respectively a groove-shaped evaporation part 12 and a tubular culture part 11, the culture part 11 is connected with the evaporation part 12 and the bottom is sealed, the culture tank 1 is placed in the water vapor collection tube 2 and matches the inner diameter width of the water vapor collection tube 2, a reflux zone 4 is spaced between the top wall of the culture part 11 and the top wall of the steam collection tube 2, the bottom end of the water vapor collection tube 2 is closed, a diagonal support piece 21 is provided at the lower part of the outer wall of the water vapor collection tube 2, and the top plug 3 is installed in the top opening of the water vapor collection tube 2 and contacts the top of the culture tank 1.

[0018] The mixed liquid and strain can be added to the culture part 11 of the culture tank 1, the culture tank 1 can be placed in the water vapor collection tube 2, the top plug 3 can close the top of the water vapor collection tube 2, and the evaporated water vapor generated during the culture process can reach the top of the water vapor collection tube 2 through the evaporation part 12 of the culture tank 1, and condense and flow back to the bottom of the water vapor collection tube 2 along the reflux area 4, thereby preventing the returned water droplets from flooding the mycelium and other bacteria and then infecting the entire culture medium. The inclined support piece 21 at the bottom of the culture tank 1 can support the culture tank 1 to tilt and culture the mother culture mycelium, thereby avoiding the problem of tilting and rotating easily during tilted placement during traditional test tube culture.

[0019] See also Figure 1-3 There is a liquid collection area 5 between the bottom of the water vapor collection tube 2 and the bottom of the culture tank 1. The liquid collection area 5 is filled with filter cotton 6. The water collected in the liquid collection area 5 can be absorbed by the filter cotton 6, which reduces secondary evaporation and facilitates subsequent cleaning.

[0020] See also Figure 1-3 The bottom of the water vapor collection tube 2 is open and sealed by a bottom plug 7, which facilitates cleaning and disinfection of the bottom of the water vapor collection tube 2.

[0021] See also Figure 1-3 The water vapor collection tube 2 is provided with rubber limiting strips 22 on both sides thereof, which are in contact with the culture tank 1 and can prevent the culture tank 1 from rotating in the water vapor collection tube 2.

[0022] Working process:

[0023] According to the needs of the strain, select a suitable culture medium to make a mixed liquid, take a clean culture tank 1 and use a dropper to absorb the mixed liquid and drip it into the culture part 11, place the filter cotton 6 at the bottom of the water vapor collection tube 2, put the culture tank 1 into the water vapor collection tube 2, and close the water vapor collection tube 2 with the top plug 3 and the bottom plug 7. Put the water vapor collection tube 2 into the sterilization box for high-temperature sterilization, support the culture tank 1 with the inclined support piece 21 to place it at an angle so that the liquid in the culture part 11 is inclined. After the disinfection is completed, take out the strain stored in the bottle, and use a sterile pipette to take a small amount of it into the culture part. 11 and then backfill the top plug 3. After completion, put it into the incubator and support it with inclined culture by the diagonal support piece 21. Under suitable temperature and humidity conditions, the culture time is 2 to 3 weeks to obtain the mother culture mycelium. The water vapor generated during the culture process flows back to the evaporation part 12 and reaches the top of the water vapor collection tube 2. The condensed water droplets flow to the liquid collection area 5 through the reflux area 4 between the culture tank 1 and the water vapor collection tube 2 and are absorbed by the filter cotton 6. After the culture is completed, take out the culture tank 1 to take out the mother culture mycelium, remove the bottom plug 7 and take out the filter cotton 6, and then disinfect the culture tank 1 and the water vapor collection tube 2.

[0024] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A mother seed mycelium culture tube, characterized in that: The invention comprises a culture tank (1), a water vapor collection tube (2) and a top plug (3); the upper section and the lower section of the culture tank (1) are respectively a trough-shaped evaporation section (12) and a tubular culture section (11); the culture section (11) is connected to the evaporation section (12) and the bottom is sealed; the culture tank (1) is placed in the water vapor collection tube (2) and matches the inner diameter width of the water vapor collection tube (2); a reflux zone (4) is provided between the top wall of the culture section (11) and the top wall of the steam collection tube (2); the bottom end of the water vapor collection tube (2) is closed; a diagonal support sheet (21) is provided at the lower part of the outer wall of the water vapor collection tube (2); and the top plug (3) is installed in the top opening of the water vapor collection tube (2) and contacts the top of the culture tank (1).

2. The mother seed mycelium culture tube according to claim 1, characterized in that: A liquid collection area (5) is provided between the bottom of the water vapor collection tube (2) and the bottom of the culture tank (1), and the liquid collection area (5) is filled with filter cotton (6).

3. The mother seed mycelium culture tube according to claim 1, characterized in that: The bottom of the water vapor collection pipe (2) is open and sealed by a bottom plug (7).

4. The mother seed mycelium culture tube according to claim 1, characterized in that: Rubber limiting strips (22) are provided on both sides of the water vapor collection tube (2) and are in contact with the culture tank (1).

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