High-flux micro-fluidic chip

Abstract

The utility model discloses a high-flux micro-fluidic chip which comprises a chip substrate, a liquid distribution flow channel provided with a first distribution flow channel and a second distribution flow channel which are isolated from each other, a microsphere preparation structure provided with a plurality of microsphere preparation modules, and a collection flow channel selectively isolated from the first distribution flow channel and / or the second distribution flow channel, the first distribution flow channel and the second distribution flow channel are correspondingly provided with a plurality of first branch flow channels and second branch flow channels respectively; the microsphere preparation module is provided with a first runner, two second runners and a microsphere output runner; the first runner is communicated with the first branch runner through a first resistance runner; the two second runners are symmetrically communicated with two sides of an output port of the first runner and are respectively communicated with the second branch runner through second resistance runners; the microsphere output runner is communicated with the output ports of the first runner and the second runner; and the collection flow channel is provided with a plurality of third branch flow channels which are respectively communicated between the microsphere output flow channel and the external collection container. According to the high-flux micro-fluidic chip, microsphere production with high monodispersity, high production speed and high efficiency can be achieved, and the production requirement of mass production of microspheres is met.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatographic filler preparation, in particular to a high-throughput microfluidic chip for producing monodisperse droplets. Background Art

[0002] Traditional microspheres are usually prepared by emulsification, which is based on the principle of mixing raw materials with incompatible solutions and additives, and then emulsifying droplets (or microspheres) by mechanical stirring or ultrasonic vibration. However, this processing method not only has low production efficiency, but also has poor monodispersity and low quality of the droplets obtained, which cannot meet the needs of mass production.

[0003] Therefore, there is an urgent need to provide a microsphere production structure that can greatly improve the production efficiency while meeting the high monodispersity of the microspheres.

[0004] In view of this, the present utility model is proposed. Summary of the invention

[0005] In order to overcome the above-mentioned defects, the utility model provides a high-throughput microfluidic chip with a simple, reasonable, novel structure and high integration. It can achieve microsphere production with high monodispersity, high production speed and efficiency, and well meet the production needs of large-scale production of microspheres.

[0006] The technical solution adopted by the utility model to solve the technical problem is: a high-throughput microfluidic chip, comprising:

[0007] Chip substrate;

[0008] The liquid distribution channel is provided with a first distribution channel and a second distribution channel which are isolated from each other and arranged on the chip substrate; the first distribution channel is provided with a plurality of first branch channels which are arranged at intervals and can be respectively communicated with an external dispersed phase liquid supply device, and the second distribution channel is provided with a plurality of second branch channels which are arranged at intervals and can be respectively communicated with an external continuous phase liquid supply device;

[0009] A microsphere preparation structure, which is provided with a plurality of microsphere preparation modules also provided on the chip substrate; each of the microsphere preparation modules is provided with a first flow channel connected with the first branch flow channel through a first resistance flow channel, two second flow channels symmetrically connected at both sides of an output port of the first flow channel and respectively connected with the second branch flow channel through a second resistance flow channel, and a microsphere output flow channel respectively connected with the output ports of the first flow channel and the second flow channel, the first resistance flow channel being able to adjust the flow rate of the dispersed phase liquid flowing into the first flow channel, the second resistance flow channel being able to adjust the flow rate of the continuous phase liquid flowing into the second flow channel, so that the continuous phase liquid in the two second flow channels can symmetrically cut the dispersed phase liquid flowing out of the first flow channel to obtain microspheres, and the obtained microspheres are output via the microsphere output flow channel;

[0010] The converging channel is also arranged on the chip substrate and is selectively isolated from the first distribution channel and / or the second distribution channel; and the converging channel is provided with a plurality of third branch channels which are arranged at intervals and respectively connected to the microsphere output channel and the external collection container.

[0011] As a further improvement of the utility model, the chip substrate comprises a first layer, a second layer and a third layer stacked in sequence;

[0012] The second distribution channel is disposed on any one of the first layer, the second layer and the third layer, and the microsphere preparation structure and the second distribution channel are located on the same layer;

[0013] The first distribution channel is also arranged on any one of the first layer, the second layer and the third layer. At the same time, the first distribution channel and the second distribution channel are not located on the same layer and are not arranged opposite to each other. In addition, the converging channel and the first distribution channel are located on the same layer.

[0014] As a further improvement of the present invention, the second branch flow channel is a U-shaped pipeline structure consisting of two straight flow channel sections extending along a first direction and arranged side by side along a second direction perpendicular to the first direction, and a connecting section connected between one ends of the two straight flow channel sections, and the other ends of the two straight flow channel sections are closed, and the connecting section is connected to an external continuous phase liquid supply device;

[0015] In addition, several of the second branch flow channels in the second distribution flow channel are arranged side by side along the second direction, and several of the microsphere preparation modules arranged at intervals along the first direction are also provided between the two straight flow channel sections in each of the second branch flow channels, that is, several of the microsphere preparation modules in the microsphere preparation structure form an array distribution in rows in the second direction and in columns in the first direction.

[0016] As a further improvement of the present invention, the plurality of first branch flow channels are straight tubular structures extending along the second direction and are arranged in parallel along the first direction to match the arrangement of the plurality of microsphere preparation modules; and, one end of each of the first branch flow channels is closed and the other end is connected to an external dispersed phase liquid supply device;

[0017] Several of the third branch flow channels are also straight tubular structures extending along the second direction and are arranged in parallel along the first direction to match the arrangement of several of the microsphere preparation modules; moreover, one end of each of the third branch flow channels is closed and the other end is connected to an external collection container.

[0018] As a further improvement of the present invention, the first distribution flow channel is further provided with a first main flow channel, and the first main flow channel can connect a plurality of the first branch flow channels to an external dispersed phase liquid supply device;

[0019] The second distribution channel is further provided with a second main channel, and the second main channel can connect the connecting sections in a plurality of the second branch channels to an external continuous phase liquid supply device;

[0020] The converging flow channel is also provided with a third main flow channel, and the third main flow channel can connect a plurality of the third branch flow channels to an external collecting container.

[0021] As a further improvement of the present invention, a plurality of the first branch flow channels and a plurality of the third branch flow channels are alternately arranged in sequence along the first direction.

[0022] As a further improvement of the present invention, the first layer, the second layer and the third layer have the same shape, and all adopt a square, circular or elliptical plate structure;

[0023] In addition, connecting holes are provided on at least two of the first layer, the second layer and the third layer for interlayer conduction and for connection with an external dispersed phase liquid supply device, an external continuous phase liquid supply device and an external collection container.

[0024] As a further improvement of the present invention, in each of the microsphere preparation modules, the inner cross-sectional area of ​​the first resistance flow channel is much smaller than the inner cross-sectional area of ​​the first flow channel, and the inner cross-sectional areas of the two second resistance flow channels are the same and much smaller than the inner cross-sectional area of ​​the second flow channel;

[0025] In the microsphere preparation structure, the inner cross-sectional areas of a plurality of the first resistance flow channels are all the same, and the inner cross-sectional areas of a plurality of the second resistance flow channels are also all the same.

[0026] As a further improvement of the present invention, in each of the microsphere preparation modules, the first flow channel, the two second flow channels and the microsphere output flow channel together form a cross channel structure; the two second resistance flow channels are arranged in mirror symmetry.

[0027] As a further improvement of the present invention, among the plurality of microsphere preparation modules connected to the same second branch flow channel, every two adjacent microsphere preparation modules are arranged in mirror symmetry, that is, every two adjacent microsphere preparation modules share a first through hole for communicating with the first branch flow channel, or share a second through hole for communicating with the third branch flow channel.

[0028] The beneficial effects of the utility model are as follows: compared with the prior art, the utility model innovatively provides a microfluidic chip, which can achieve microsphere production with high monodispersity (i.e. high consistency), high production speed and high efficiency, such as: the microsphere production can reach an output of 1 to 5 L / h, which fully reaches the industrial production level and can well meet the production needs of mass production of microspheres. In addition, the microfluidic chip provided by the utility model has a simple, reasonable, novel structure, high integration, and is easy to process and manufacture. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the exploded structure of the high-throughput microfluidic chip of the utility model;

[0030] Figure 2 This is a schematic diagram of the structure in which the second distribution channel and the microsphere preparation structure are arranged on the second layer of the utility model;

[0031] Figure 3 for Figure 2 The enlarged structural diagram of the A part is shown in FIG.

[0032] Figure 4 for Figure 3 Schematic diagram of the enlarged structure of the microsphere preparation module shown in;

[0033] Figure 5 This is a schematic diagram of the structure in which the first distribution channel and the converging channel are arranged on the third layer of the utility model.

[0034] Combined with the accompanying drawings, the following description is given:

[0035] 1. Chip substrate; 10. First layer; 100. Connecting hole A; 11. Second layer;

[0036] 110. Connecting hole B; 12. Third layer; 2. First distribution channel; 20. First branch channel; 21. First main channel; 3. Second distribution channel; 30. Second branch channel; 300. Direct flow section; 301. Connecting section; 31. Second main channel; 4. Microsphere preparation module; 40. First resistance channel; 41. First channel; 42. Second resistance channel; 43. Second channel; 44. Microsphere output channel; 45. First through hole; 46. Second through hole; 5. Converging channel; 50. Third branch channel; 51. Third main channel. DETAILED DESCRIPTION

[0037] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0038] Embodiment 1:

[0039] Please refer to the attached Figure 1 To Attachment Figure 5 As shown, this embodiment 1 provides a high-throughput microfluidic chip, which includes a chip substrate 1, a liquid distribution channel, a microsphere preparation structure and a converging channel 5, wherein the liquid distribution channel is provided with a first distribution channel 2 and a second distribution channel 3 which are isolated from each other and are arranged on the chip substrate 1, the first distribution channel 2 is provided with a plurality of first branch channels 20 which are arranged at intervals and can be respectively connected to an external dispersed phase liquid supply device, and the second distribution channel 3 is provided with a plurality of second branch channels 30 which are arranged at intervals and can be respectively connected to an external continuous phase liquid supply device; the microsphere preparation structure is provided with a plurality of microsphere preparation modules 4 which are also provided on the chip substrate 1, each of the microsphere preparation modules 4 is provided with a first channel 41 which is connected to the first branch channel 20 through a first resistance channel 40, two second channels 43 which are symmetrically connected to both sides of the output port of the first channel 41 and are respectively connected to the second branch channel 30 through a second resistance channel 42, and The output ports of the first flow channel 41 and the second flow channel 43 are connected to the microsphere output flow channel 44. The first resistance flow channel 40 can adjust the flow rate of the dispersed phase liquid flowing into the first flow channel 41, and the second resistance flow channel 42 can adjust the flow rate of the continuous phase liquid flowing into the second flow channel 43. By means of the regulating effect of the first resistance flow channel 40 and the second resistance flow channel 42, it can be achieved that the continuous phase liquid in the two second flow channels 43 can efficiently and symmetrically cut the dispersed phase liquid flowing out of the first flow channel 41 to obtain a large number of microspheres with regular shapes and high monodispersity. The obtained microspheres are output through the microsphere output flow channel 44; the converging flow channel 5 is also arranged on the chip substrate 1, and is selectively isolated from the first distribution flow channel 2 and / or the second distribution flow channel 3, and the converging flow channel 5 is provided with a plurality of third branch flow channels 50 arranged at intervals and respectively connected between the microsphere output flow channel 44 and the external collection container.

[0040] It can be understood that the working principle of the high-throughput microfluidic chip described in this embodiment 1 is: S1) the dispersed phase liquid (such as water phase) supplied by the external dispersed phase liquid supply device flows through the first distribution channel 2 and the first resistance channel 40 in sequence, and then flows into the first channel 41; the continuous phase liquid (such as oil phase) supplied by the external continuous phase liquid supply device flows through the second distribution channel 3 and the two second resistance channels 42 in sequence, and then flows into the two second channels 43 at a constant flow rate; S2) the continuous phase liquid in the two second channels 43 efficiently and symmetrically cuts the dispersed phase liquid flowing out of the first channel 41 to obtain a large number of microspheres with regular shapes and high monodispersity, and the obtained microspheres flow through the microsphere output channel 44 and the converging channel 5 in sequence, and then merge into the external collection container.

[0041] The specific structure of the high-throughput microfluidic chip provided in this embodiment is described in detail below.

[0042] First, the layout design of the liquid distribution channel, the microsphere preparation structure and the collection channel 5 on the chip substrate 1 is described.

[0043] Please continue to refer to the attached Figure 1 As shown, the chip substrate 1 in this embodiment preferably adopts a three-layer plate structure, that is, the chip substrate 1 includes a first layer 10, a second layer 11 and a third layer 12 stacked in sequence; wherein the materials of the first layer 10, the second layer 11 and the third layer 12 are designed to be the same material, such as: glass material can be preferably used; and the shapes of the first layer 10, the second layer 11 and the third layer 12 are also designed to be the same shape, such as: square, circular or elliptical plate structure can be preferably used. Of course, the present application is not limited to the above materials and shapes, which are determined according to the product design requirements.

[0044] Based on the three-layer plate structure of the chip substrate 1, ① the second distribution channel 3 can be arranged on any one of the first layer 10, the second layer 11 and the third layer 12, Figure 1 and attached Figure 2 ② The first distribution channel 2 can also be arranged on any one of the first layer 10, the second layer 11 and the third layer 12, but at the same time, it is required that the first distribution channel 2 and the second distribution channel 3 are not located on the same layer and are not arranged opposite to each other; Figure 13 shows the situation where the first distribution channel 2 is arranged on the front of the third layer 12; it is understandable that when the layout of the second distribution channel 3 changes, the layout of the first distribution channel 2 needs to be changed accordingly. ③ The microsphere preparation structure is designed to be located on the same layer as the second distribution channel 3, that is, the microsphere preparation structure is also preferably arranged on the front of the second layer 11. ④ The converging channel 5 is designed to be located on the same layer as the first distribution channel 2, that is, the converging channel 5 is also preferably arranged on the front of the third layer 12.

[0045] This embodiment optimizes the layout of the liquid distribution channel, the microsphere preparation structure and the collection channel 5, so that the limited area of ​​the chip substrate can be fully utilized, thereby achieving the purpose of improving the chip structure integration.

[0046] In addition, additional explanations: ① In actual application, in addition to the above-mentioned three-layer plate structure, the chip substrate 1 can also adopt a four-layer plate structure. Specifically, based on the three-layer plate structure of the above-mentioned chip substrate 1, the second layer 11 is configured as two layers. At that time, the first resistance flow channel 40 and the second resistance flow channel 42 in the microsphere preparation module 4 will be respectively arranged on different second layers 11. Such a design can further reduce the footprint of the microsphere preparation structure, thereby further improving the overall integration of the chip structure. ② Based on the layout of the above-mentioned liquid distribution flow channel, microsphere preparation structure and converging flow channel 5, the second layer 11 can also be defined as a "microsphere preparation layer" and the third layer 12 can be defined as a "liquid separation and infusion layer".

[0047] Next, the structures of the liquid distributing channel and the collecting channel 5 will be described.

[0048] Based on the above description of the structure and shape of the chip substrate 1, the preferred implementation structure of the second distribution channel 3 in this embodiment is: Figure 1 To Attachment Figure 3 As shown, the second distribution channel 3 is provided with a plurality of second branch channels 30 and a second main channel 31, wherein each of the second branch channels 30 is a U-shaped pipeline structure consisting of two straight channel sections 300 extending along the first direction D1 and arranged side by side along the second direction D2 perpendicular to the first direction D1, and a connecting section 301 connected between one ends of the two straight channel sections 300, and the other ends of the two straight channel sections 300 are closed, and the connecting section 301 is connected to an external continuous phase liquid supply device through the second main channel 31; and the plurality of second branch channels 30 in the second distribution channel 3 are also arranged side by side along the second direction D2. It can be understood that when the second layer 11 adopts a square plate structure, the structural design of the second distribution channel 3 can achieve the maximum area utilization.

[0049] In addition, the above-mentioned “the connecting section 301 is connected to the external continuous phase liquid supply device through the second main channel 31” can be understood as: the second main channel 31 can connect the connecting sections 301 in several of the second branch channels 30 to the external continuous phase liquid supply device; the specific connection structure is: the second main channel 31 is connected to the external continuous phase liquid supply device, and the connecting sections 301 in several of the second branch channels 30 are connected to the second main channel 31.

[0050] In addition, since the microsphere preparation structure and the second distribution channel 3 are located in the same layer, based on the above-mentioned implementation structure of the second distribution channel 3, the layout of the microsphere preparation structure can be further optimized as follows: Figure 2 As shown, between the two straight channel sections 300 in each of the second branch channels 30, there are provided a plurality of microsphere preparation modules 4 arranged at intervals along the first direction D1, that is, the plurality of microsphere preparation modules 4 in the microsphere preparation structure can form an array distribution in which the second direction D2 is arranged in rows and the first direction D1 is arranged in columns. It can be understood that, by coordinating and optimizing the shape and structure of the chip substrate 1, the structure of the second distribution channel 3, and the layout setting of the microsphere preparation structure, a large number of microsphere preparation modules 4 can be integrated on the limited area of ​​the chip substrate 1, thereby greatly improving the microsphere production speed in the field of microfluidic technology, that is, improving the production efficiency of the microfluidic chip. In this embodiment, the microspheres produced by the microfluidic chip can reach a production of 1 to 5 L / h, which fully reaches the industrial-grade production level and well meets the production needs of mass production of microspheres.

[0051] Based on the implementation structure of the second distribution channel 3 and the layout of the microsphere preparation structure, the preferred implementation structure of the first distribution channel 2 in this embodiment is: Figure 1 and attached Figure 5 As shown, the first distribution channel 2 includes a plurality of first branch channels 20 and a first main channel 21, wherein the plurality of first branch channels 20 are straight tubular structures extending along the second direction D2, and are arranged in parallel along the first direction D1 at the same time, so as to match the arrangement of the plurality of microsphere preparation modules 4; moreover, one end of each of the first branch channels 20 is closed, and the other end is connected to an external dispersed phase liquid supply device through the first main channel 21; that is, the first main channel 21 can connect the plurality of first branch channels 20 to an external dispersed phase liquid supply device.

[0052] The preferred implementation structure of the converging flow channel 5 in this embodiment is: Figure 1 and attached Figure 5 As shown, the converging channel 5 includes a plurality of third branch channels 50 and a third main channel 51, wherein the plurality of third branch channels 50 are also straight tubular structures extending along the second direction D2, and are simultaneously arranged in parallel along the first direction D1 to match the arrangement of the plurality of microsphere preparation modules 4; moreover, one end of each of the third branch channels 50 is closed, and the other end is connected to the external collection container through the third main channel 51, that is, the third main channel 51 can connect the plurality of third branch channels 50 to the external collection container.

[0053] In summary, the implementation structures of the first distribution channel 2 and the collection channel 5 are designed based on the layout of the microsphere preparation structure to better supply dispersed phase liquid to the microsphere preparation module 4 and collect microspheres.

[0054] In addition, since the first distribution channel 2 and the converging channel 5 are arranged on the same layer (i.e., the third layer 12), in order to avoid mutual interference between the first distribution channel 2 and the converging channel 5, and to improve space utilization and reduce chip volume, the present embodiment further performs the following structural optimization on the first distribution channel 2 and the converging channel 5: Figure 5 As shown, the plurality of first branch flow channels 20 in the first distribution flow channel 2 and the plurality of third branch flow channels 50 in the converging flow channel 5 are alternately arranged in sequence and at intervals along the first direction D1.

[0055] In addition, based on the above description of the structure of the liquid distribution channel and the collection channel 5, this embodiment also provides connecting holes on at least two of the first layer 10, the second layer 11 and the third layer 12 of the chip substrate 1 for inter-layer conduction and for connection with an external dispersed phase liquid supply device, an external continuous phase liquid supply device and an external collection container. For details, please refer to the attached Figure 1 As shown, the first layer 10 is provided with three connecting holes A100 for connecting with an external dispersed phase liquid supply device, an external collecting container, and an external continuous phase liquid supply device respectively; the second layer 11 is provided with a connecting hole B110 for inter-layer conduction, and so on.

[0056] Next, the structure of the microsphere preparation structure is described.

[0057] Please continue to refer to the attached Figure 2 To Attachment Figure 4As shown, in this embodiment, the inner cross-sectional area of ​​the first resistance flow channel 40 in each of the microsphere preparation modules 4 is much smaller than the inner cross-sectional area of ​​the first flow channel 41, and the inner cross-sectional areas of the two second resistance flow channels 42 are the same and much smaller than the inner cross-sectional area of ​​the second flow channel 43; at the same time, the inner cross-sectional areas of several first resistance flow channels 40 in the microsphere preparation structure are the same, and the inner cross-sectional areas of several second resistance flow channels 42 are also the same. That is, in this embodiment, the first resistance flow channel 40 and the second resistance flow channel 42 are both formed by the circuitous microflow channel to ensure that the liquid / fluid transported through the first distribution flow channel 2 and the second distribution flow channel 3 is evenly distributed in different microsphere preparation modules 4, so as to complete the cutting action with high consistency and high symmetry, thereby greatly improving the monodispersity of microsphere preparation. In addition, by setting the first resistance flow channel 40 and the second resistance flow channel 42, the influence of the blockage of a single flow channel on the flow of other flow channels can also be reduced, thereby improving the service life and utilization rate of the chip.

[0058] It is understandable that the adjustment of the liquid flow rate by the first resistance flow channel 40 and the second resistance flow channel 42 is specifically manifested in: the magnitude of the resistance generated by the first resistance flow channel 40 and the second resistance flow channel 42 to the liquid. Therefore, in order to achieve the monodispersity requirements of the microspheres required for production, that is, the resistance / liquid flow rate requirements, this can be achieved by designing and adjusting the inner cross-sectional area, length, and circuitous shape of the first resistance flow channel 40 and the second resistance flow channel 42 (technical personnel in this field can easily obtain the technical parameters of the first resistance flow channel 40 and the second resistance flow channel 42 through knowledge of fluid mechanics, so they will not be described in detail here). In addition, supplementary explanation: In the chip structure described in this embodiment, all positions where turns appear on all flow channels are designed to be arc-shaped to reduce the generation of vortices, turbulence, etc.

[0059] Please continue to refer to the attached Figure 2 To Attachment Figure 4 As shown, in this embodiment, the first flow channel 41, the two second flow channels 43 and the microsphere output flow channel 44 in each of the microsphere preparation modules 4 together form a cross channel structure, and the two second resistance flow channels 42 are arranged in mirror symmetry. Moreover, in the plurality of microsphere preparation modules 4 connected to the same second branch flow channel 30, each two adjacent microsphere preparation modules 4 are arranged in mirror symmetry, that is: each two adjacent microsphere preparation modules 4 share a first through hole 45 for communicating with the first branch flow channel 20, or share a second through hole 46 for communicating with the third branch flow channel 50. It can be understood that based on the above-mentioned optimization of the structure and connection relationship of the microsphere preparation module 4, it is convenient to achieve symmetrical cutting to obtain microspheres, and it also saves space well, which is conducive to the integrated layout of the product structure.

[0060] In summary, compared with the prior art, the utility model innovatively provides a microfluidic chip, which can achieve microsphere production with high monodispersity (i.e. high consistency), high production speed and high efficiency, such as: the microsphere production can reach an output of 1 to 5 L / h, which fully reaches the industrial production level and can well meet the production needs of mass production of microspheres. In addition, the microfluidic chip provided by the utility model has a simple, reasonable, novel structure, high integration, and is easy to process and manufacture.

[0061] In the above description, many specific details are described to facilitate a full understanding of the present invention. However, the above description is only a preferred embodiment of the present invention. The present invention can be implemented in many other ways different from those described herein, so the present invention is not limited by the specific implementation disclosed above. At the same time, any person familiar with the technical field can make many possible changes and modifications to the technical solution of the present invention by using the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the scope of protection of the technical solution of the present invention.

Claims

1. A high-throughput microfluidic chip, characterized in that: include: Chip substrate (1); A liquid distribution channel, comprising a first distribution channel (2) and a second distribution channel (3) which are isolated from each other and arranged on the chip substrate (1); the first distribution channel (2) is provided with a plurality of first branch channels (20) which are arranged at intervals and can be respectively communicated with an external dispersed phase liquid supply device, and the second distribution channel (3) is provided with a plurality of second branch channels (30) which are arranged at intervals and can be respectively communicated with an external continuous phase liquid supply device; A microsphere preparation structure, comprising a plurality of microsphere preparation modules (4) also arranged on the chip substrate (1); each of the microsphere preparation modules (4) is provided with a first flow channel (41) connected to the first branch flow channel (20) via a first resistance flow channel (40), two second flow channels (43) symmetrically connected to both sides of an output port of the first flow channel (41) and respectively connected to the second branch flow channel (30) via second resistance flow channels (42), and two second flow channels (43) respectively connected to the first flow channel (41) and the second flow channel (43). The first resistance flow channel (40) is capable of adjusting the flow rate of the dispersed phase liquid flowing into the first flow channel (41), and the second resistance flow channel (42) is capable of adjusting the flow rate of the continuous phase liquid flowing into the second flow channel (43), so that the continuous phase liquid in the two second flow channels (43) can symmetrically cut the dispersed phase liquid flowing out of the first flow channel (41) to obtain microspheres, and the obtained microspheres are output through the microsphere output flow channel (44); A converging flow channel (5) is also provided on the chip substrate (1) and is selectively isolated from the first distribution flow channel (2) and / or the second distribution flow channel (3); and the converging flow channel (5) is provided with a plurality of third branch flow channels (50) arranged at intervals and respectively connected between the microsphere output flow channel (44) and the external collection container.

2. The high-throughput microfluidic chip according to claim 1, characterized in that: The chip substrate (1) comprises a first layer (10), a second layer (11) and a third layer (12) which are stacked in sequence; The second distribution channel (3) is arranged on any one of the first layer (10), the second layer (11) and the third layer (12), and the microsphere preparation structure and the second distribution channel (3) are located on the same layer; The first distribution channel (2) is also arranged on any one of the first layer (10), the second layer (11) and the third layer (12), and the first distribution channel (2) and the second distribution channel (3) are not located on the same layer and are not arranged opposite to each other; in addition, the converging channel (5) and the first distribution channel (2) are located on the same layer.

3. The high-throughput microfluidic chip according to claim 2, characterized in that: The second branch flow channel (30) is a U-shaped pipeline structure consisting of two straight flow channel sections (300) extending along a first direction and arranged side by side along a second direction perpendicular to the first direction, and a connecting section (301) connected between one ends of the two straight flow channel sections (300), and the other ends of the two straight flow channel sections (300) are closed, and the connecting section (301) is connected to an external continuous phase liquid supply device; In addition, a plurality of the second branch flow channels (30) in the second distribution flow channel (3) are arranged side by side along the second direction, and a plurality of the microsphere preparation modules (4) arranged at intervals along the first direction are provided between the two straight flow channel sections (300) in each of the second branch flow channels (30), that is, the plurality of the microsphere preparation modules (4) in the microsphere preparation structure form an array distribution in rows in the second direction and in columns in the first direction.

4. The high-throughput microfluidic chip according to claim 3, characterized in that: The plurality of first branch flow channels (20) are all straight tubular structures extending along the second direction and are arranged in parallel along the first direction to match the arrangement of the plurality of microsphere preparation modules (4); and one end of each of the first branch flow channels (20) is closed and the other end is connected to an external dispersed phase liquid supply device; The plurality of third branch flow channels (50) are also straight tubular structures extending along the second direction and are arranged in parallel along the first direction to match the arrangement of the plurality of microsphere preparation modules (4); moreover, one end of each of the third branch flow channels (50) is closed and the other end is connected to an external collection container.

5. The high-throughput microfluidic chip according to claim 4, characterized in that: The first distribution flow channel (2) is further provided with a first main flow channel (21), and the first main flow channel (21) is capable of connecting a plurality of the first branch flow channels (20) to an external dispersed phase liquid supply device; The second distribution channel (3) is further provided with a second main channel (31), and the second main channel (31) is capable of connecting the connecting sections (301) in a plurality of the second branch channels (30) to an external continuous phase liquid supply device; The converging flow channel (5) is further provided with a third main flow channel (51), and the third main flow channel (51) is capable of connecting a plurality of the third branch flow channels (50) to an external collection container.

6. The high-throughput microfluidic chip according to claim 4, characterized in that: A plurality of the first branch flow channels (20) and a plurality of the third branch flow channels (50) are alternately arranged in sequence along the first direction.

7. The high-throughput microfluidic chip according to claim 4, characterized in that: The first layer (10), the second layer (11) and the third layer (12) have the same shape, and all adopt a square, circular or elliptical plate structure; In addition, connecting holes are provided on at least two of the first layer (10), the second layer (11) and the third layer (12) for inter-layer conduction and for connection with an external dispersed phase liquid supply device, an external continuous phase liquid supply device and an external collection container.

8. The high-throughput microfluidic chip according to claim 1, characterized in that: In each of the microsphere preparation modules (4), the inner cross-sectional area of ​​the first resistance flow channel (40) is much smaller than the inner cross-sectional area of ​​the first flow channel (41), and the inner cross-sectional areas of the two second resistance flow channels (42) are the same and are both much smaller than the inner cross-sectional area of ​​the second flow channel (43); In the microsphere preparation structure, the inner cross-sectional areas of a plurality of the first resistance flow channels (40) are all the same, and the inner cross-sectional areas of a plurality of the second resistance flow channels (42) are also all the same.

9. The high-throughput microfluidic chip according to claim 1, characterized in that: In each of the microsphere preparation modules (4), the first flow channel (41), the two second flow channels (43) and the microsphere output flow channel (44) together form a cross channel structure; the two second resistance flow channels (42) are arranged in a mirror-symmetrical manner.

10. The high-throughput microfluidic chip according to claim 4, characterized in that: Among the plurality of microsphere preparation modules (4) connected to the same second branch flow channel (30), every two adjacent microsphere preparation modules (4) are arranged in a mirror-symmetrical manner, that is, every two adjacent microsphere preparation modules (4) share a first through hole (45) for communicating with the first branch flow channel (20), or share a second through hole (46) for communicating with the third branch flow channel (50).

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