1536-hole DNA automatic liquid separation synthesis plate
By designing a 1536-well DNA automatic liquid separation synthetic plate, using slope structure and multiple liquid addition methods, the problem that existing DNA synthetic plates cannot meet the massive DNA needs, and efficient DNA synthesis and efficiency improvement are achieved.
Patent Information
- Application Number
- CN202323610976.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-28
- Publication Date
- 2025-01-07
- Estimated Expiration
- 2033-12-28
AI Technical Summary
The existing DNA synthetic plate specifications (such as single columns, 24, 48, 96, 384) cannot meet the market's demand for massive DNA synthesis, especially as the synthesis scale continues to expand, it cannot effectively improve the synthesis efficiency and unit area. Synthesis ability.
A 1536-hole DNA automatic liquid separation synthetic plate was designed. By setting a slope at the mouth of the synthesis chamber, its diameter was increased so that the liquid could slide into the synthesis column, and a slope was formed at the connection between the hole distribution area and the non-hole distribution area. Concentrate excess liquid to avoid contamination, and is compatible with spray or immersion liquid addition.
It significantly improves the synthesis capacity and synthesis efficiency per unit area, ensures that the liquid enters the synthesis column smoothly, avoids residual surface pollution, is compatible with a variety of liquid addition methods, and improves the efficiency of industrial synthesis.
Smart Images
Figure CN222313131U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to an ultra-trace DNA synthesis plate, in particular to a 1536-well DNA automatic liquid separation synthesis plate. Background Art
[0002] With the rapid development of synthetic biology, artificial life, functional genomics, proteomics, target drug synthesis, nucleotide interference drugs, DNA-encoded compound libraries, and clinical diagnostic research, the industrial synthesis of massive DNA is needed, and the rapid and industrial synthesis of targeted drugs, nucleotide interference drugs, and DNA-encoded compound libraries has gradually become a hot topic in the development and screening of new drugs. It will inevitably put forward higher functional requirements for the synthesis column plate of the CPG body of DNA synthesis.
[0003] At present, the common synthesis scales range from single column, 24, 48, 96, 384 scale synthesis plates, where the CPG body is loaded into the appropriate assembly position in the empty column tube of the synthesis column and placed in the matching DNA synthesizer for DNA synthesis. As the demand for synthesis scale increases, these specifications of synthesis plates are gradually unable to meet market demand. Utility Model Content
[0004] In view of the problem that the current specifications of synthesis plates cannot meet the market demand, the utility model provides a 1536-well DNA automatic liquid separation synthesis plate, which greatly improves the synthesis capacity per unit area and improves the synthesis efficiency.
[0005] In order to achieve the above object, the utility model provides a 1536-well DNA automatic liquid separation synthesis plate, which comprises:
[0006] ontology;
[0007] 1536 synthesis chambers are arranged in a matrix on the body, and the mouths are formed with slopes so that the area between adjacent synthesis chambers tends to zero;
[0008] A synthesis column, disposed in the synthesis chamber, comprises:
[0009] The inlet is connected to the bottom of the synthesis chamber, and the diameter gradually decreases from the connection point to the end.
[0010] An assembly unit, used for assembling a CPG carrier;
[0011] Export department.
[0012] Preferably, a slope is formed at the connection between the hole-distributing area of the body and the non-hole-distributing area surrounding it, so that the top surface of the hole-distributing area is higher than the top surface of the non-hole-distributing area.
[0013] Preferably, the synthesis chamber is square or circular.
[0014] Preferably, an arc connection is formed at a connection between the synthesis chamber and the inlet portion.
[0015] Preferably, the outlet portion forms a closed end, a straight end or a flared end from the connection with the assembly portion to the end.
[0016] Preferably, a positioning edge is formed on the body.
[0017] Through the above technical solution, the utility model achieves the following beneficial effects:
[0018] 1. The 1536-well DNA automatic liquid separation synthesis plate provided by the utility model greatly improves the synthesis capacity per unit area. By setting a slope at the mouth of the synthesis chamber and increasing its mouth diameter, the liquid can slide into the synthesis chamber after falling on the body, and then smoothly enter the synthesis column, so that the upper and lower parts can be separated; it is compatible with spraying or immersion liquid addition methods, which greatly improves the synthesis efficiency.
[0019] 2. In a preferred technical solution of the present invention, a slope is formed at the connection between the non-porous area and the porous area of the main body so that the top surface of the porous area is higher than the top surface of the non-porous area, so that excess liquid is concentrated in the non-porous area and then overflows through the drainage port of the synthesis equipment to avoid remaining on the synthesis plate and affecting the next step of synthesis. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 This is a schematic diagram of the structure of the 1536-well DNA automatic liquid separation synthesis plate of the utility model;
[0021] Figure 2 yes Figure 1 Bottom view of
[0022] Figure 3 yes Figure 1 AA section view;
[0023] Figure 4 yes Figure 3 A magnified schematic diagram of point B in the middle.
[0024] Description of Reference Numerals
[0025] 1 main body, 11 positioning edge, 2 synthesis chamber, 3 synthesis column, 31 entrance part, 32 assembly part, 33 exit part. DETAILED DESCRIPTION
[0026] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.
[0027] First of all, it should be noted that in the following description, some directional words, such as "external" and "internal", etc., are used to clearly explain the technical solution of the utility model. They are all analogous to the normal directions of the parts in the 1536-well DNA automatic liquid separation synthesis plate. For example, the part where the liquid passes is the internal part, and the part opposite to it is the external part. It is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation of the utility model.
[0028] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0029] like Figure 1-Figure 4 As shown, the 1536-well DNA automatic liquid separation synthesis plate described in the utility model includes:
[0030] Ontology 1;
[0031] 1536 synthesis chambers 2 are arranged in a matrix on the body 1, and the mouths are formed with slopes so that the area between adjacent synthesis chambers 2 tends to 0;
[0032] The synthesis column 3 is arranged in the synthesis chamber 2 and comprises:
[0033] The inlet 31 is connected to the bottom of the synthesis chamber 2, and the diameter gradually decreases from the connection point to the end.
[0034] An assembly unit 32, used for assembling a CPG carrier;
[0035] Exit portion 33.
[0036] Small-sized synthetic plates, such as single-column, 24, 48, 96, and 384-scale synthetic plates, usually use V-shaped needles to add liquid. Since the number of synthetic chambers 2 in the present invention reaches 1536 and the size is large, the density of the synthetic chambers 2 is also large, and the efficiency of adding liquid with V-shaped needles is low. In order to solve this problem, the pharmaceutical liquid is slowly sprayed or flooded onto the synthetic plate by spraying or flooding. If the spray or liquid falls on a plane, it is not easy to fall into the synthetic chamber 2 and remain on the surface of the main body 1 to cause pollution. Therefore, the inner wall of the synthetic chamber 2 is set to a slope, and the diameter of its mouth is increased, so that after the liquid falls on the main body 1, it can slide into the synthetic chamber 2 and then smoothly enter the synthetic column 3 to achieve liquid separation. In combination with continuous liquid supply equipment, efficient synthesis is achieved.
[0037] The 1536-well DNA automatic liquid separation synthesis plate provided by the utility model greatly improves the synthesis capacity per unit area. By arranging a slope at the mouth of the synthesis chamber 2 to increase its mouth diameter, the liquid can slide into the synthesis chamber 2 after falling on the main body 1, and then smoothly enter the synthesis column 3, so that the liquid on the synthesis plate main body 1 can be separated, so that the liquid sprayed or flooded on the surface of the main body 1 falls into the synthesis chamber 2, and will not remain on the surface to cause pollution. It is compatible with spraying or immersion liquid adding methods, and greatly improves the synthesis efficiency.
[0038] In order to avoid contamination caused by the pharmaceutical liquid sprayed or overflowed on the surface of the main body 1, a slope is formed at the connection between the perforated area of the main body 1 and the non-perforated area around it, so that the top surface of the perforated area is higher than the top surface of the non-perforated area, so that excess liquid can be concentrated in the non-perforated area and then overflow through the drainage port of the synthesis equipment to avoid remaining on the synthesis plate and affecting the next step of synthesis.
[0039] As some preferred embodiments, the synthesis chamber 2 is square or circular.
[0040] The connection between the synthesis chamber 2 and the inlet portion 31 forms an arc connection, so that the liquid can fall freely into the hole.
[0041] The outlet 33 is usually in a closed form, that is, the diameter of the outlet 33 gradually decreases from the connection with the assembly part 32 to the end, which is conducive to controlling the change of the liquid level. Because the utility model is a 1536 specification, the aperture is already small, and the outlet 33 can also be in a straight or expanded form (that is, the diameter gradually increases from the connection with the assembly part 32 to the end). A positioning edge 11 is formed on the body 1 to facilitate the rapid positioning of the synthetic board on the synthetic equipment.
[0042] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, and these simple modifications all belong to the protection scope of the present invention.
[0043] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present utility model will not further describe various possible combinations.
[0044] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.
Claims
1. A 1536-well DNA automatic liquid separation synthesis plate, characterized in that: include: Ontology(1); 1536 synthesis chambers (2) are arranged in a matrix on the body (1), and the mouths are formed with a slope so that the area of the region between adjacent synthesis chambers (2) tends to zero; The synthesis column (3) is arranged in the synthesis chamber (2), and comprises: The inlet portion (31) is connected to the bottom of the synthesis chamber (2), and the diameter gradually decreases from the connection point to the end. An assembly unit (32), used for assembling a CPG carrier; Exit portion (33).
2. The 1536-well DNA automatic liquid separation synthesis plate according to claim 1, characterized in that: A slope is formed at the connection between the hole-distributing area of the body (1) and the surrounding non-hole-distributing area, so that the top surface of the hole-distributing area is higher than the top surface of the non-hole-distributing area.
3. The 1536-well DNA automatic liquid separation synthesis plate according to claim 1, characterized in that: The synthesis chamber (2) is square or circular.
4. The 1536-well DNA automatic liquid separation synthesis plate according to claim 1, characterized in that: An arc-shaped connection is formed at the connection between the synthesis chamber (2) and the inlet portion (31).
5. The 1536-well DNA automatic liquid separation synthesis plate according to claim 1, characterized in that: The outlet portion (33) forms a closed end, a straight end or a flared end from the connection with the assembly portion (32) to the end.
6. The 1536-well DNA automatic liquid separation synthesis plate according to any one of claims 1 to 5, characterized in that: A positioning edge (11) is formed on the main body (1).